P47108 (URB2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nucleolar pre-ribosomal-associated protein 2

Alternative name(s):
Unhealthy ribosome biogenesis protein 2

Gene names

Name:	URB2
Synonyms:	NPA2
Ordered Locus Names:	YJR041C
ORF Names:	J1622

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	1174 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for 27S pre-rRNA processing. Has an early role during 60S ribosomal subunit assembly. Ref.6 Ref.8
Subunit structure	Component of very early pre-60S ribosomal particles containing 27SA2 pre-rRNA. Interacts with URB1. Together with DBP6, NOP8, URB1 and RSA3, forms an RNA-independent complex, which is required during early maturation of nascent 60S ribosomal subunits. Ref.6 Ref.7 Ref.8
Subcellular location	Nucleus › nucleolus Ref.4 Ref.7 Ref.8.
Miscellaneous	Present with 1660 molecules/cell in log phase SD medium.

Ontologies

Keywords
Biological process	Ribosome biogenesis rRNA processing
Cellular component	Nucleus
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	rRNA metabolic process Inferred from mutant phenotype Ref.6. Source: SGD rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW ribosome biogenesis Inferred from physical interaction Ref.7. Source: SGD
Cellular_component	nucleolus Inferred from direct assay Ref.4Ref.7. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1174	1174	Nucleolar pre-ribosomal-associated protein 2		PRO_0000203094

Sequences

	Sequence		Length	Mass (Da)
	P47108 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 7647D48DA557D4D4	FASTA	1,174	135,118
	10 20 30 40 50 60 MGDLTEELSI PDNAQDLSKL LRSTSTKPHQ IAEIVSKFDK LETYFPKKEI FVLDLLIDRL 70 80 90 100 110 120 NNGNLDDFKT SEHTWIIFTR LLDAINDPIS IKKLLKKLKT VPVMIRTFFL WPKDKLLTRS 130 140 150 160 170 180 VSFIKAFFAI NDYLIVNFSV EESFQLLEHA INGLSSCPTT DFALSYLQDA CNLTHVDNIT 190 200 210 220 230 240 TTDNKIATCY CKHMLLPSLR YFAQTKNSAS SNQSFIRLSH FMGKFLLQPR IDYMKLNKKF 250 260 270 280 290 300 VQENASEITD DMAYYYFATF VTFLSKDNFA QLEVIFTILG AKKPSLECRF LNLLSESKKT 310 320 330 340 350 360 VSQEFLEALL LEMLASTDES GVLSLIPIIL KLDIEVAIKH IFRLLELIQL ENLNDPLFSS 370 380 390 400 410 420 HIWDLIIQSH ANARELSDFF AKINEYCSRK GPDSYFLINH PAYVKSITKQ LFTLSSLQWK 430 440 450 460 470 480 NLLQALLDQV NHDSTNRVPL YLIRICLEGL SEGASRATLD EVKPILSQVF TLESFNNSLQ 490 500 510 520 530 540 WDLKYHIMEV YDDIVPAEEL EKIDYVLSSN IFDTTSADVE ELFFYCFKLR EYISFDLSDA 550 560 570 580 590 600 KKKFMRHFEI LDEERKSNLS YSVVSKFATL VNNNFTREQI SSLIDSLLLN STNLSSLLKN 610 620 630 640 650 660 DDIFEETNIT YALINKLASS YHQTFALEAL IQIPIQCINK NVRVALINNL TCESFCLDSA 670 680 690 700 710 720 TRECLLHLLS SPTFKSNIET NFYELCEKTI MSPEMAISET GDEKKEIEDK ISIFEKVWTN 730 740 750 760 770 780 HLSQAKEPVS EKFLESGYDI VKQSMSLSNG DSKLIIAGFT IAKFLKPDNK HRDIQGMAIS 790 800 810 820 830 840 YAVKILENYS ENFESETIPL FRISMSTLYK IITTGQGDIS KHKSRILDIF SKIMLRYHSK 850 860 870 880 890 900 KVYHAPEEQE MFLVHSLLTE NKLEYIFAEY LNIEHTDKCD SALGFCLEES LKQGPDAFNR 910 920 930 940 950 960 LLWNSAKSFS TISQPCAEKF VRVFIIMSKR IARDNNLGHH LFVIALLEAY TYCDIEKFGY 970 980 990 1000 1010 1020 KSYLLLFNAI KEFLVSKPWL FSQYCIEMLL PFCLKTLAFI VNHESTDEIN EGFINIIEVI 1030 1040 1050 1060 1070 1080 DHMLLVHRFK FSNRHHLFNS VLCQILEIIA IHDGTLCANS ADAVARLITN YCEPYNVSNA 1090 1100 1110 1120 1130 1140 QNGQKNNLSS KISLIKQSIR KNVLVVLTKY IQLSITTQFS LNIKKSLQPG IHAIFDILSQ 1150 1160 1170 NELNQLNAFL DTPGKQYFKA LYLQYKKVGK WRED

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of a 42.5 kb DNA sequence of chromosome X reveals three tRNA genes and 14 new open reading frames including a gene most probably belonging to the family of ubiquitin-protein ligases." Huang M.-E., Chuat J.-C., Galibert F. Yeast 11:775-781(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Exploration of essential gene functions via titratable promoter alleles." Mnaimneh S., Davierwala A.P., Haynes J., Moffat J., Peng W.-T., Zhang W., Yang X., Pootoolal J., Chua G., Lopez A., Trochesset M., Morse D., Krogan N.J., Hiley S.L., Li Z., Morris Q., Grigull J., Mitsakakis N. , Roberts C.J., Greenblatt J.F., Boone C., Kaiser C.A., Andrews B.J., Hughes T.R. Cell 118:31-44(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH URB1.
[7]	"Npa1p, a component of very early pre-60S ribosomal particles, associates with a subset of small nucleolar RNPs required for peptidyl transferase center modification." Dez C., Froment C., Noaillac-Depeyre J., Monsarrat B., Caizergues-Ferrer M., Henry Y. Mol. Cell. Biol. 24:6324-6337(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH URB1, SUBCELLULAR LOCATION.
[8]	"Characterization of Saccharomyces cerevisiae Npa2p (Urb2p) reveals a Low-molecular-mass complex containing Dbp6p, Npa1p (Urb1p), Nop8p, and Rsa3p involved in early steps of 60S ribosomal subunit biogenesis." Rosado I.V., Dez C., Lebaron S., Caizergues-Ferrer M., Henry Y., de la Cruz J. Mol. Cell. Biol. 27:1207-1221(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBUNIT, IDENTIFICATION IN A COMPLEX WITH DBP6; NOP8; URB1 AND RSA3, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L36344 Genomic DNA. Translation: AAA88743.1. Z49541 Genomic DNA. Translation: CAA89568.1. Z49542 Genomic DNA. Translation: CAA89570.1. BK006943 Genomic DNA. Translation: DAA08828.1.
PIR	S57060.
RefSeq	NP_012575.3. NM_001181699.3.
3D structure databases
ProteinModelPortal	P47108.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6348N.
IntAct	P47108. 5 interactions.
MINT	MINT-2492459.
STRING	4932.YJR041C.
Proteomic databases
PaxDb	P47108.
PeptideAtlas	P47108.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJR041C; YJR041C; YJR041C.
GeneID	853498.
KEGG	sce:YJR041C.
Organism-specific databases
CYGD	YJR041c.
SGD	S000003802. URB2.
Phylogenomic databases
eggNOG	NOG67574.
KO	K14862.
OMA	IIDRWND.
OrthoDB	EOG44N21P.
Enzyme and pathway databases
BioCyc	YEAST:G3O-31676-MONOMER.
Gene expression databases
Genevestigator	P47108.
GermOnline	YJR041C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR018849. Urb2/Npa2_C. [Graphical view]
Pfam	PF10441. Urb2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	974137.

Entry information

Entry name

URB2_YEAST

Accession

Primary (citable) accession number: P47108
Secondary accession number(s): D6VWL2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	May 29, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program