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P47108 (URB2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nucleolar pre-ribosomal-associated protein 2
Alternative name(s):
Unhealthy ribosome biogenesis protein 2
Gene names
Name:URB2
Synonyms:NPA2
Ordered Locus Names:YJR041C
ORF Names:J1622
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1174 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for 27S pre-rRNA processing. Has an early role during 60S ribosomal subunit assembly. Ref.6 Ref.8

Subunit structure

Component of very early pre-60S ribosomal particles containing 27SA2 pre-rRNA. Interacts with URB1. Together with DBP6, NOP8, URB1 and RSA3, forms an RNA-independent complex, which is required during early maturation of nascent 60S ribosomal subunits. Ref.6 Ref.7 Ref.8

Subcellular location

Nucleusnucleolus Ref.4 Ref.7 Ref.8.

Miscellaneous

Present with 1660 molecules/cell in log phase SD medium.

Ontologies

Keywords
   Biological processRibosome biogenesis
rRNA processing
   Cellular componentNucleus
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processrRNA metabolic process

Inferred from mutant phenotype Ref.6. Source: SGD

rRNA processing

Inferred from electronic annotation. Source: UniProtKB-KW

ribosome biogenesis

Inferred from physical interaction Ref.7. Source: SGD

   Cellular_componentnucleolus

Inferred from direct assay Ref.7. Source: SGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11741174Nucleolar pre-ribosomal-associated protein 2
PRO_0000203094

Sequences

Sequence LengthMass (Da)Tools
P47108 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: 7647D48DA557D4D4

FASTA1,174135,118
        10         20         30         40         50         60 
MGDLTEELSI PDNAQDLSKL LRSTSTKPHQ IAEIVSKFDK LETYFPKKEI FVLDLLIDRL 

        70         80         90        100        110        120 
NNGNLDDFKT SEHTWIIFTR LLDAINDPIS IKKLLKKLKT VPVMIRTFFL WPKDKLLTRS 

       130        140        150        160        170        180 
VSFIKAFFAI NDYLIVNFSV EESFQLLEHA INGLSSCPTT DFALSYLQDA CNLTHVDNIT 

       190        200        210        220        230        240 
TTDNKIATCY CKHMLLPSLR YFAQTKNSAS SNQSFIRLSH FMGKFLLQPR IDYMKLNKKF 

       250        260        270        280        290        300 
VQENASEITD DMAYYYFATF VTFLSKDNFA QLEVIFTILG AKKPSLECRF LNLLSESKKT 

       310        320        330        340        350        360 
VSQEFLEALL LEMLASTDES GVLSLIPIIL KLDIEVAIKH IFRLLELIQL ENLNDPLFSS 

       370        380        390        400        410        420 
HIWDLIIQSH ANARELSDFF AKINEYCSRK GPDSYFLINH PAYVKSITKQ LFTLSSLQWK 

       430        440        450        460        470        480 
NLLQALLDQV NHDSTNRVPL YLIRICLEGL SEGASRATLD EVKPILSQVF TLESFNNSLQ 

       490        500        510        520        530        540 
WDLKYHIMEV YDDIVPAEEL EKIDYVLSSN IFDTTSADVE ELFFYCFKLR EYISFDLSDA 

       550        560        570        580        590        600 
KKKFMRHFEI LDEERKSNLS YSVVSKFATL VNNNFTREQI SSLIDSLLLN STNLSSLLKN 

       610        620        630        640        650        660 
DDIFEETNIT YALINKLASS YHQTFALEAL IQIPIQCINK NVRVALINNL TCESFCLDSA 

       670        680        690        700        710        720 
TRECLLHLLS SPTFKSNIET NFYELCEKTI MSPEMAISET GDEKKEIEDK ISIFEKVWTN 

       730        740        750        760        770        780 
HLSQAKEPVS EKFLESGYDI VKQSMSLSNG DSKLIIAGFT IAKFLKPDNK HRDIQGMAIS 

       790        800        810        820        830        840 
YAVKILENYS ENFESETIPL FRISMSTLYK IITTGQGDIS KHKSRILDIF SKIMLRYHSK 

       850        860        870        880        890        900 
KVYHAPEEQE MFLVHSLLTE NKLEYIFAEY LNIEHTDKCD SALGFCLEES LKQGPDAFNR 

       910        920        930        940        950        960 
LLWNSAKSFS TISQPCAEKF VRVFIIMSKR IARDNNLGHH LFVIALLEAY TYCDIEKFGY 

       970        980        990       1000       1010       1020 
KSYLLLFNAI KEFLVSKPWL FSQYCIEMLL PFCLKTLAFI VNHESTDEIN EGFINIIEVI 

      1030       1040       1050       1060       1070       1080 
DHMLLVHRFK FSNRHHLFNS VLCQILEIIA IHDGTLCANS ADAVARLITN YCEPYNVSNA 

      1090       1100       1110       1120       1130       1140 
QNGQKNNLSS KISLIKQSIR KNVLVVLTKY IQLSITTQFS LNIKKSLQPG IHAIFDILSQ 

      1150       1160       1170 
NELNQLNAFL DTPGKQYFKA LYLQYKKVGK WRED 

« Hide

References

« Hide 'large scale' references
[1]"Analysis of a 42.5 kb DNA sequence of chromosome X reveals three tRNA genes and 14 new open reading frames including a gene most probably belonging to the family of ubiquitin-protein ligases."
Huang M.-E., Chuat J.-C., Galibert F.
Yeast 11:775-781(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"Exploration of essential gene functions via titratable promoter alleles."
Mnaimneh S., Davierwala A.P., Haynes J., Moffat J., Peng W.-T., Zhang W., Yang X., Pootoolal J., Chua G., Lopez A., Trochesset M., Morse D., Krogan N.J., Hiley S.L., Li Z., Morris Q., Grigull J., Mitsakakis N. expand/collapse author list , Roberts C.J., Greenblatt J.F., Boone C., Kaiser C.A., Andrews B.J., Hughes T.R.
Cell 118:31-44(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH URB1.
[7]"Npa1p, a component of very early pre-60S ribosomal particles, associates with a subset of small nucleolar RNPs required for peptidyl transferase center modification."
Dez C., Froment C., Noaillac-Depeyre J., Monsarrat B., Caizergues-Ferrer M., Henry Y.
Mol. Cell. Biol. 24:6324-6337(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH URB1, SUBCELLULAR LOCATION.
[8]"Characterization of Saccharomyces cerevisiae Npa2p (Urb2p) reveals a Low-molecular-mass complex containing Dbp6p, Npa1p (Urb1p), Nop8p, and Rsa3p involved in early steps of 60S ribosomal subunit biogenesis."
Rosado I.V., Dez C., Lebaron S., Caizergues-Ferrer M., Henry Y., de la Cruz J.
Mol. Cell. Biol. 27:1207-1221(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT, IDENTIFICATION IN A COMPLEX WITH DBP6; NOP8; URB1 AND RSA3, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L36344 Genomic DNA. Translation: AAA88743.1.
Z49541 Genomic DNA. Translation: CAA89568.1.
Z49542 Genomic DNA. Translation: CAA89570.1.
BK006943 Genomic DNA. Translation: DAA08828.1.
PIRS57060.
RefSeqNP_012575.3. NM_001181699.3.

3D structure databases

ProteinModelPortalP47108.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33792. 25 interactions.
DIPDIP-6348N.
IntActP47108. 4 interactions.
MINTMINT-2492459.
STRING4932.YJR041C.

Proteomic databases

PaxDbP47108.
PeptideAtlasP47108.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJR041C; YJR041C; YJR041C.
GeneID853498.
KEGGsce:YJR041C.

Organism-specific databases

CYGDYJR041c.
SGDS000003802. URB2.

Phylogenomic databases

eggNOGNOG67574.
KOK14862.
OMADIFSIIW.
OrthoDBEOG7RRFGB.

Enzyme and pathway databases

BioCycYEAST:G3O-31676-MONOMER.

Gene expression databases

GenevestigatorP47108.

Family and domain databases

InterProIPR018849. Urb2/Npa2_C.
[Graphical view]
PfamPF10441. Urb2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio974137.

Entry information

Entry nameURB2_YEAST
AccessionPrimary (citable) accession number: P47108
Secondary accession number(s): D6VWL2
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: February 19, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD