ID   RAV1_YEAST              Reviewed;        1357 AA.
AC   P47104; D6VWK6;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   27-MAR-2024, entry version 185.
DE   RecName: Full=Regulator of V-ATPase in vacuolar membrane protein 1;
DE   AltName: Full=Suppression of the onset of impotence protein 3;
GN   Name=RAV1; Synonyms=SOI3; OrderedLocusNames=YJR033C; ORFNames=J1590;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=8641269; DOI=10.1002/j.1460-2075.1996.tb00557.x;
RA   Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C.,
RA   Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D.,
RA   Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J.,
RA   Heumann K., Hilger F., Hollenberg C.P., Huang M.-E., Jacq C.,
RA   Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E.,
RA   Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T.,
RA   Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B.,
RA   Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R.,
RA   Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N.,
RA   To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H.,
RA   von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.;
RT   "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.";
RL   EMBO J. 15:2031-2049(1996).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-1016.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=7668047; DOI=10.1002/yea.320110809;
RA   Huang M.-E., Chuat J.-C., Galibert F.;
RT   "Analysis of a 42.5 kb DNA sequence of chromosome X reveals three tRNA
RT   genes and 14 new open reading frames including a gene most probably
RT   belonging to the family of ubiquitin-protein ligases.";
RL   Yeast 11:775-781(1995).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 728-1357.
RX   PubMed=8619316; DOI=10.1002/yea.320111208;
RA   Zagulski M., Babinska B., Gromadka R., Migdalski A., Rytka J., Sulicka J.,
RA   Herbert C.J.;
RT   "The sequence of 24.3 kb from chromosome X reveals five complete open
RT   reading frames, all of which correspond to new genes, and a tandem
RT   insertion of a Ty1 transposon.";
RL   Yeast 11:1179-1186(1995).
RN   [5]
RP   FUNCTION, IDENTIFICATION IN THE RAVE COMPLEX WITH RAV2 AND CBF3D, AND
RP   INTERACTION WITH RAV2; CBF3D; VMA1; VMA2; VMA4 AND VMA8.
RX   PubMed=11283612; DOI=10.1038/35070067;
RA   Seol J.H., Shevchenko A., Shevchenko A., Deshaies R.J.;
RT   "Skp1 forms multiple protein complexes, including RAVE, a regulator of V-
RT   ATPase assembly.";
RL   Nat. Cell Biol. 3:384-391(2001).
RN   [6]
RP   FUNCTION OF THE RAVE COMPLEX.
RX   PubMed=11844802; DOI=10.1074/jbc.m200682200;
RA   Smardon A.M., Tarsio M., Kane P.M.;
RT   "The RAVE complex is essential for stable assembly of the yeast V-ATPase.";
RL   J. Biol. Chem. 277:13831-13839(2002).
RN   [7]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=15090613; DOI=10.1091/mbc.e03-10-0755;
RA   Sipos G., Brickner J.H., Brace E.J., Chen L., Rambourg A., Kepes F.,
RA   Fuller R.S.;
RT   "Soi3p/Rav1p functions at the early endosome to regulate endocytic
RT   trafficking to the vacuole and localization of trans-Golgi network
RT   transmembrane proteins.";
RL   Mol. Biol. Cell 15:3196-3209(2004).
RN   [8]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [9]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1244, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=YAL6B;
RX   PubMed=15665377; DOI=10.1074/mcp.m400219-mcp200;
RA   Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M.,
RA   Jensen O.N.;
RT   "Quantitative phosphoproteomics applied to the yeast pheromone signaling
RT   pathway.";
RL   Mol. Cell. Proteomics 4:310-327(2005).
RN   [10]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1244 AND SER-1248, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA   Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT   "A multidimensional chromatography technology for in-depth phosphoproteome
RT   analysis.";
RL   Mol. Cell. Proteomics 7:1389-1396(2008).
RN   [11]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1244 AND SER-1248, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=19779198; DOI=10.1126/science.1172867;
RA   Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT   "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT   into evolution.";
RL   Science 325:1682-1686(2009).
CC   -!- FUNCTION: Component of the RAVE complex, which is required for stable
CC       assembly of the vacuolar ATPase complex V-ATPase under many conditions.
CC       Required for transport between the early endosome and the late
CC       endosome/prevacuolar compartment (PVC), suggesting that assembly of
CC       vacuolar ATPase at the early endosome is required for transport from
CC       the early endosome to the PVC. {ECO:0000269|PubMed:11283612,
CC       ECO:0000269|PubMed:11844802, ECO:0000269|PubMed:15090613}.
CC   -!- SUBUNIT: Component of the RAVE complex composed of RAV1, RAV2 and
CC       CBF3D/SKP1. Within the complex, it interacts directly with RAV2 and
CC       CBF3D. Interacts with the V-ATPase V1 subunits VMA1, VMA2 and VMA8.
CC       {ECO:0000269|PubMed:11283612}.
CC   -!- INTERACTION:
CC       P47104; P52286: SKP1; NbExp=5; IntAct=EBI-25471, EBI-4090;
CC       P47104; P17255: VMA1; NbExp=2; IntAct=EBI-25471, EBI-20245;
CC       P47104; P16140: VMA2; NbExp=3; IntAct=EBI-25471, EBI-20254;
CC       P47104; P22203: VMA4; NbExp=3; IntAct=EBI-25471, EBI-20268;
CC       P47104; P32610: VMA8; NbExp=2; IntAct=EBI-25471, EBI-20264;
CC   -!- SUBCELLULAR LOCATION: Endomembrane system
CC       {ECO:0000269|PubMed:15090613}. Note=Probably membrane-associated,
CC       associates with high density membrane compartment like early endosomes.
CC   -!- MISCELLANEOUS: Present with 149 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
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DR   EMBL; Z49533; CAA89560.1; -; Genomic_DNA.
DR   EMBL; L36344; AAA88735.1; -; Genomic_DNA.
DR   EMBL; X87297; CAA60726.1; -; Genomic_DNA.
DR   EMBL; BK006943; DAA08822.1; -; Genomic_DNA.
DR   PIR; S57052; S57052.
DR   RefSeq; NP_012567.3; NM_001181691.3.
DR   AlphaFoldDB; P47104; -.
DR   BioGRID; 33786; 279.
DR   ComplexPortal; CPX-1627; RAVE complex.
DR   DIP; DIP-916N; -.
DR   IntAct; P47104; 6.
DR   MINT; P47104; -.
DR   STRING; 4932.YJR033C; -.
DR   CarbonylDB; P47104; -.
DR   iPTMnet; P47104; -.
DR   MaxQB; P47104; -.
DR   PaxDb; 4932-YJR033C; -.
DR   PeptideAtlas; P47104; -.
DR   TopDownProteomics; P47104; -.
DR   EnsemblFungi; YJR033C_mRNA; YJR033C; YJR033C.
DR   GeneID; 853490; -.
DR   KEGG; sce:YJR033C; -.
DR   AGR; SGD:S000003794; -.
DR   SGD; S000003794; RAV1.
DR   VEuPathDB; FungiDB:YJR033C; -.
DR   eggNOG; KOG1064; Eukaryota.
DR   GeneTree; ENSGT00390000000096; -.
DR   HOGENOM; CLU_000310_0_1_1; -.
DR   InParanoid; P47104; -.
DR   OMA; NSHLTLW; -.
DR   OrthoDB; 5480252at2759; -.
DR   BioCyc; YEAST:G3O-31670-MONOMER; -.
DR   BioGRID-ORCS; 853490; 6 hits in 10 CRISPR screens.
DR   PRO; PR:P47104; -.
DR   Proteomes; UP000002311; Chromosome X.
DR   RNAct; P47104; Protein.
DR   GO; GO:0005737; C:cytoplasm; IDA:SGD.
DR   GO; GO:0005829; C:cytosol; IEA:GOC.
DR   GO; GO:0012505; C:endomembrane system; NAS:ComplexPortal.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0043291; C:RAVE complex; IPI:SGD.
DR   GO; GO:0045022; P:early endosome to late endosome transport; IMP:SGD.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   GO; GO:0043254; P:regulation of protein-containing complex assembly; IMP:SGD.
DR   GO; GO:0007035; P:vacuolar acidification; IMP:SGD.
DR   GO; GO:0070072; P:vacuolar proton-transporting V-type ATPase complex assembly; IDA:ComplexPortal.
DR   Gene3D; 2.130.10.10; YVTN repeat-like/Quinoprotein amine dehydrogenase; 2.
DR   InterPro; IPR022033; Rav1p_C.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   InterPro; IPR001680; WD40_rpt.
DR   PANTHER; PTHR13950:SF9; RABCONNECTIN-3A; 1.
DR   PANTHER; PTHR13950; RABCONNECTIN-RELATED; 1.
DR   Pfam; PF12234; Rav1p_C; 1.
DR   SMART; SM00320; WD40; 5.
DR   SUPFAM; SSF50978; WD40 repeat-like; 2.
PE   1: Evidence at protein level;
KW   Membrane; Phosphoprotein; Protein transport; Reference proteome; Repeat;
KW   Transport; WD repeat.
FT   CHAIN           1..1357
FT                   /note="Regulator of V-ATPase in vacuolar membrane protein
FT                   1"
FT                   /id="PRO_0000051183"
FT   REPEAT          98..134
FT                   /note="WD 1"
FT   REPEAT          142..182
FT                   /note="WD 2"
FT   REPEAT          190..239
FT                   /note="WD 3"
FT   REPEAT          384..423
FT                   /note="WD 4"
FT   REPEAT          431..470
FT                   /note="WD 5"
FT   REPEAT          595..636
FT                   /note="WD 6"
FT   REPEAT          638..679
FT                   /note="WD 7"
FT   REPEAT          898..939
FT                   /note="WD 8"
FT   REGION          1243..1357
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1265..1316
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1317..1340
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         1244
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:15665377,
FT                   ECO:0007744|PubMed:18407956, ECO:0007744|PubMed:19779198"
FT   MOD_RES         1248
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:18407956,
FT                   ECO:0007744|PubMed:19779198"
SQ   SEQUENCE   1357 AA;  154933 MW;  6B7270B6901274CF CRC64;
     MSLNFLPGRP NATPQTACQA TWQNHTIFAY CSGNNLIILT NKFTRLQTIY TQSDCTAVDI
     NSQNGFIALS FHNRVLIYKP IHQIMQNPKW TQCCQLFHDD TPVNCLRWSS DNELAIGSDF
     LSFWKIKDNF GVYQPILQWN QKQPKPVYNV IISQDSQLIV SIGKYDCNAK LWKRVSIVGE
     QAIFNLTMLP HPKPITAMRW KKEPDQVSKN NTASHALYTL CEDKVLRIWS CFEMEKNHTV
     QIWGEVPLSP TQKFCVIIDN WIIRQTLSVK DSEIFDISDS DIVILGSMTG EMEVLALNNL
     SQDPPKPMTK KTISHKKVKK ATMLNDTRYL YLPEIQPYDN VKGKLSFLVH DLQGVIRHLL
     IDILQLINNK TEDLSAALEH KFTGHNKSVQ KLVRSSDGEA LLTTSRFSEN GVWYPQKLNH
     GVSLRLQNTI QTESPIKFAV VHELGKQVIC LLENGALQAW ECPTNRKEDS EQKQSYLRVE
     TRLKEEKKIH PIVMLNTPEP KHSHERHFTA LIFSDGSIKA FEVSLTRGIF EVKSDSLDID
     GDDIYKISII DPVHQTFVSN RPLISLITKK GLTRTYKAIV NYNDRHVQWI KACEINTGIM
     NCTCIRGSST GKLCIVNSTG KVMSLWDLNR GVLEYEETFH NPIEDIDWTS TEYGQSIVSI
     GFTGYALLYT QLRYDYTNNT PSYLPIEKID ITAHTAHNIG DSVWMKNGTF VVASGNQFYI
     KDKSLDLTDP FTYQSIGSRK ILSNDILHLS SVLNGPLPVY HPQFLIQAIY ANKLQLVKEL
     LLRLFLALRK LDFESQDVSN LDSNLGMDPL KYFIAKDRDY PVESFPDPYP CFNKTVSLAL
     TEQLTKTTLP YLTRHQQITL ITVIEAVDEV TKNENIVDYN GVRFLLGVKL FLSHKNIQKS
     ILMRDVSWAL HSDNKEILLS SIDRHITSWN RAREYRIAYW IKEQDLVKKF EDIAKYEFSK
     DDKRDPSRCA IFYLALKKKQ ILLSLWKMAI GHPEQQKMVR FISNDFTVPR WRTAALKNAF
     VLLSKHRYMD AAVFFLLTDS LKDCVNVLCK QVHDMDLAIG VCRVYEGDNG PVLGELLTAQ
     MLPETIKEND RWKASFIYWK LRKQEVAIKA LLTAPIDLEN NSSIVDKEVC VNRSFLVEDP
     ALLYLYNHLR NRNLKYFIGS LNVEAKIECT LILRVTDILC RMGCNYLAVS LVKNWKFIER
     NSIPVQKLLK SPTKDRAYSA IGAMASEPIS TARMRPSLFD KFGSPSASDI ESPNPKLPNS
     LLDDFLQPPP NSTSSNSLAQ SSSSAPRSIL DEFVSPSYSQ HKENLTPKAP NDSVGETDNS
     ENRKDKLSKD ILDDLSSQKP QKPKKSAITK NLLDDFV
//