P47104 (RAV1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Regulator of V-ATPase in vacuolar membrane protein 1

Alternative name(s):
Suppression of the onset of impotence protein 3

Gene names

Name:	RAV1
Synonyms:	SOI3
Ordered Locus Names:	YJR033C
ORF Names:	J1590

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	1357 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the RAVE complex, which is required for stable assembly of the vacuolar APTase complex V-ATPase under many conditions. Required for transport between the early endosome and the late endosome/prevacuolar compartment (PVC), suggesting that assembly of vacuolar ATPase at the early endosome is required for transport from the early endosome to the PVC. Ref.5 Ref.6 Ref.7
Subunit structure	Component of the RAVE complex composed of RAV1, RAV2 and CBF3D/SKP1. Within the complex, it interacts directly with RAV2 and CBF3D. Interacts with the V-ATPase V1 subunits VMA1, VMA2 and VMA8. Ref.5
Subcellular location	Endomembrane system. Note: Probably membrane-associated, associates with high density membrane compartment like early endosomes. Ref.7
Miscellaneous	Present with 149 molecules/cell in log phase SD medium.
Sequence similarities	Contains 8 WD repeats.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Membrane
Domain	Repeat WD repeat
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	early endosome to late endosome transport Inferred from mutant phenotype Ref.7. Source: SGD protein transport Inferred from electronic annotation. Source: UniProtKB-KW regulation of protein complex assembly Inferred from mutant phenotype Ref.5Ref.6. Source: SGD vacuolar acidification Inferred from mutant phenotype Ref.5Ref.6. Source: SGD
Cellular_component	RAVE complex Inferred from physical interaction Ref.5Ref.6. Source: SGD endomembrane system Inferred from electronic annotation. Source: UniProtKB-SubCell extrinsic to membrane Inferred from direct assay Ref.7. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SKP1	P52286	5	EBI-25471,EBI-4090
VMA1	P17255	2	EBI-25471,EBI-20245
VMA2	P16140	4	EBI-25471,EBI-20254
VMA4	P22203	3	EBI-25471,EBI-20268
VMA8	P32610	2	EBI-25471,EBI-20264

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1357	1357	Regulator of V-ATPase in vacuolar membrane protein 1		PRO_0000051183
Regions
	Repeat	98 – 134	37	WD 1
	Repeat	142 – 182	41	WD 2
	Repeat	190 – 239	50	WD 3
	Repeat	384 – 423	40	WD 4
	Repeat	431 – 470	40	WD 5
	Repeat	595 – 636	42	WD 6
	Repeat	638 – 679	42	WD 7
	Repeat	898 – 939	42	WD 8
Amino acid modifications
	Modified residue	1219	1	Phosphoserine Ref.10
	Modified residue	1244	1	Phosphoserine Ref.9 Ref.10
	Modified residue	1248	1	Phosphoserine Ref.10

Sequences

	Sequence		Length	Mass (Da)
	P47104 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 6B7270B6901274CF	FASTA	1,357	154,933
	10 20 30 40 50 60 MSLNFLPGRP NATPQTACQA TWQNHTIFAY CSGNNLIILT NKFTRLQTIY TQSDCTAVDI 70 80 90 100 110 120 NSQNGFIALS FHNRVLIYKP IHQIMQNPKW TQCCQLFHDD TPVNCLRWSS DNELAIGSDF 130 140 150 160 170 180 LSFWKIKDNF GVYQPILQWN QKQPKPVYNV IISQDSQLIV SIGKYDCNAK LWKRVSIVGE 190 200 210 220 230 240 QAIFNLTMLP HPKPITAMRW KKEPDQVSKN NTASHALYTL CEDKVLRIWS CFEMEKNHTV 250 260 270 280 290 300 QIWGEVPLSP TQKFCVIIDN WIIRQTLSVK DSEIFDISDS DIVILGSMTG EMEVLALNNL 310 320 330 340 350 360 SQDPPKPMTK KTISHKKVKK ATMLNDTRYL YLPEIQPYDN VKGKLSFLVH DLQGVIRHLL 370 380 390 400 410 420 IDILQLINNK TEDLSAALEH KFTGHNKSVQ KLVRSSDGEA LLTTSRFSEN GVWYPQKLNH 430 440 450 460 470 480 GVSLRLQNTI QTESPIKFAV VHELGKQVIC LLENGALQAW ECPTNRKEDS EQKQSYLRVE 490 500 510 520 530 540 TRLKEEKKIH PIVMLNTPEP KHSHERHFTA LIFSDGSIKA FEVSLTRGIF EVKSDSLDID 550 560 570 580 590 600 GDDIYKISII DPVHQTFVSN RPLISLITKK GLTRTYKAIV NYNDRHVQWI KACEINTGIM 610 620 630 640 650 660 NCTCIRGSST GKLCIVNSTG KVMSLWDLNR GVLEYEETFH NPIEDIDWTS TEYGQSIVSI 670 680 690 700 710 720 GFTGYALLYT QLRYDYTNNT PSYLPIEKID ITAHTAHNIG DSVWMKNGTF VVASGNQFYI 730 740 750 760 770 780 KDKSLDLTDP FTYQSIGSRK ILSNDILHLS SVLNGPLPVY HPQFLIQAIY ANKLQLVKEL 790 800 810 820 830 840 LLRLFLALRK LDFESQDVSN LDSNLGMDPL KYFIAKDRDY PVESFPDPYP CFNKTVSLAL 850 860 870 880 890 900 TEQLTKTTLP YLTRHQQITL ITVIEAVDEV TKNENIVDYN GVRFLLGVKL FLSHKNIQKS 910 920 930 940 950 960 ILMRDVSWAL HSDNKEILLS SIDRHITSWN RAREYRIAYW IKEQDLVKKF EDIAKYEFSK 970 980 990 1000 1010 1020 DDKRDPSRCA IFYLALKKKQ ILLSLWKMAI GHPEQQKMVR FISNDFTVPR WRTAALKNAF 1030 1040 1050 1060 1070 1080 VLLSKHRYMD AAVFFLLTDS LKDCVNVLCK QVHDMDLAIG VCRVYEGDNG PVLGELLTAQ 1090 1100 1110 1120 1130 1140 MLPETIKEND RWKASFIYWK LRKQEVAIKA LLTAPIDLEN NSSIVDKEVC VNRSFLVEDP 1150 1160 1170 1180 1190 1200 ALLYLYNHLR NRNLKYFIGS LNVEAKIECT LILRVTDILC RMGCNYLAVS LVKNWKFIER 1210 1220 1230 1240 1250 1260 NSIPVQKLLK SPTKDRAYSA IGAMASEPIS TARMRPSLFD KFGSPSASDI ESPNPKLPNS 1270 1280 1290 1300 1310 1320 LLDDFLQPPP NSTSSNSLAQ SSSSAPRSIL DEFVSPSYSQ HKENLTPKAP NDSVGETDNS 1330 1340 1350 ENRKDKLSKD ILDDLSSQKP QKPKKSAITK NLLDDFV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Analysis of a 42.5 kb DNA sequence of chromosome X reveals three tRNA genes and 14 new open reading frames including a gene most probably belonging to the family of ubiquitin-protein ligases." Huang M.-E., Chuat J.-C., Galibert F. Yeast 11:775-781(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-1016. Strain: ATCC 204508 / S288c.
[4]	"The sequence of 24.3 kb from chromosome X reveals five complete open reading frames, all of which correspond to new genes, and a tandem insertion of a Ty1 transposon." Zagulski M., Babinska B., Gromadka R., Migdalski A., Rytka J., Sulicka J., Herbert C.J. Yeast 11:1179-1186(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 728-1357.
[5]	"Skp1 forms multiple protein complexes, including RAVE, a regulator of V-ATPase assembly." Seol J.H., Shevchenko A., Shevchenko A., Deshaies R.J. Nat. Cell Biol. 3:384-391(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN THE RAVE COMPLEX WITH RAV2 AND CBF3D, INTERACTION WITH RAV2; CBF3D; VMA1; VMA2; VMA4 AND VMA8.
[6]	"The RAVE complex is essential for stable assembly of the yeast V-ATPase." Smardon A.M., Tarsio M., Kane P.M. J. Biol. Chem. 277:13831-13839(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION OF THE RAVE COMPLEX.
[7]	"Soi3p/Rav1p functions at the early endosome to regulate endocytic trafficking to the vacuole and localization of trans-Golgi network transmembrane proteins." Sipos G., Brickner J.H., Brace E.J., Chen L., Rambourg A., Kepes F., Fuller R.S. Mol. Biol. Cell 15:3196-3209(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1244, MASS SPECTROMETRY. Strain: YAL6B.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1219; SER-1244 AND SER-1248, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z49533 Genomic DNA. Translation: CAA89560.1. L36344 Genomic DNA. Translation: AAA88735.1. X87297 Genomic DNA. Translation: CAA60726.1. BK006943 Genomic DNA. Translation: DAA08822.1.
PIR	S57052.
RefSeq	NP_012567.3. NM_001181691.3.
3D structure databases
ProteinModelPortal	P47104.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-916N.
IntAct	P47104. 6 interactions.
MINT	MINT-668932.
STRING	4932.YJR033C.
Proteomic databases
PaxDb	P47104.
PeptideAtlas	P47104.
PRIDE	P47104.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJR033C; YJR033C; YJR033C.
GeneID	853490.
KEGG	sce:YJR033C.
Organism-specific databases
CYGD	YJR033c.
SGD	S000003794. RAV1.
Phylogenomic databases
eggNOG	NOG314136.
GeneTree	ENSGT00390000000096.
HOGENOM	HOG000093808.
OMA	TQLRYDY.
OrthoDB	EOG4JDMGD.
Enzyme and pathway databases
BioCyc	YEAST:G3O-31670-MONOMER.
Gene expression databases
Genevestigator	P47104.
GermOnline	YJR033C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	2.130.10.10. 4 hits.
InterPro	IPR022033. Rav1p_C. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR017986. WD40_repeat_dom. [Graphical view]
Pfam	PF12234. Rav1p_C. 1 hit. [Graphical view]
SMART	SM00320. WD40. 5 hits. [Graphical view]
SUPFAM	SSF50978. WD40_like. 2 hits.
PROSITE	PS00678. WD_REPEATS_1. False negative. PS50082. WD_REPEATS_2. False negative. PS50294. WD_REPEATS_REGION. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	974119.

Entry information

Entry name

RAV1_YEAST

Accession

Primary (citable) accession number: P47104
Secondary accession number(s): D6VWK6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	May 29, 2013
This is version 110 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program