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P47104 (RAV1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Regulator of V-ATPase in vacuolar membrane protein 1
Alternative name(s):
Suppression of the onset of impotence protein 3
Gene names
Name:RAV1
Synonyms:SOI3
Ordered Locus Names:YJR033C
ORF Names:J1590
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1357 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the RAVE complex, which is required for stable assembly of the vacuolar ATPase complex V-ATPase under many conditions. Required for transport between the early endosome and the late endosome/prevacuolar compartment (PVC), suggesting that assembly of vacuolar ATPase at the early endosome is required for transport from the early endosome to the PVC. Ref.5 Ref.6 Ref.7

Subunit structure

Component of the RAVE complex composed of RAV1, RAV2 and CBF3D/SKP1. Within the complex, it interacts directly with RAV2 and CBF3D. Interacts with the V-ATPase V1 subunits VMA1, VMA2 and VMA8. Ref.5

Subcellular location

Endomembrane system. Note: Probably membrane-associated, associates with high density membrane compartment like early endosomes. Ref.7

Miscellaneous

Present with 149 molecules/cell in log phase SD medium.

Sequence similarities

Contains 8 WD repeats.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 13571357Regulator of V-ATPase in vacuolar membrane protein 1
PRO_0000051183

Regions

Repeat98 – 13437WD 1
Repeat142 – 18241WD 2
Repeat190 – 23950WD 3
Repeat384 – 42340WD 4
Repeat431 – 47040WD 5
Repeat595 – 63642WD 6
Repeat638 – 67942WD 7
Repeat898 – 93942WD 8

Amino acid modifications

Modified residue12441Phosphoserine Ref.9 Ref.10 Ref.11
Modified residue12481Phosphoserine Ref.10 Ref.11

Sequences

Sequence LengthMass (Da)Tools
P47104 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: 6B7270B6901274CF

FASTA1,357154,933
        10         20         30         40         50         60 
MSLNFLPGRP NATPQTACQA TWQNHTIFAY CSGNNLIILT NKFTRLQTIY TQSDCTAVDI 

        70         80         90        100        110        120 
NSQNGFIALS FHNRVLIYKP IHQIMQNPKW TQCCQLFHDD TPVNCLRWSS DNELAIGSDF 

       130        140        150        160        170        180 
LSFWKIKDNF GVYQPILQWN QKQPKPVYNV IISQDSQLIV SIGKYDCNAK LWKRVSIVGE 

       190        200        210        220        230        240 
QAIFNLTMLP HPKPITAMRW KKEPDQVSKN NTASHALYTL CEDKVLRIWS CFEMEKNHTV 

       250        260        270        280        290        300 
QIWGEVPLSP TQKFCVIIDN WIIRQTLSVK DSEIFDISDS DIVILGSMTG EMEVLALNNL 

       310        320        330        340        350        360 
SQDPPKPMTK KTISHKKVKK ATMLNDTRYL YLPEIQPYDN VKGKLSFLVH DLQGVIRHLL 

       370        380        390        400        410        420 
IDILQLINNK TEDLSAALEH KFTGHNKSVQ KLVRSSDGEA LLTTSRFSEN GVWYPQKLNH 

       430        440        450        460        470        480 
GVSLRLQNTI QTESPIKFAV VHELGKQVIC LLENGALQAW ECPTNRKEDS EQKQSYLRVE 

       490        500        510        520        530        540 
TRLKEEKKIH PIVMLNTPEP KHSHERHFTA LIFSDGSIKA FEVSLTRGIF EVKSDSLDID 

       550        560        570        580        590        600 
GDDIYKISII DPVHQTFVSN RPLISLITKK GLTRTYKAIV NYNDRHVQWI KACEINTGIM 

       610        620        630        640        650        660 
NCTCIRGSST GKLCIVNSTG KVMSLWDLNR GVLEYEETFH NPIEDIDWTS TEYGQSIVSI 

       670        680        690        700        710        720 
GFTGYALLYT QLRYDYTNNT PSYLPIEKID ITAHTAHNIG DSVWMKNGTF VVASGNQFYI 

       730        740        750        760        770        780 
KDKSLDLTDP FTYQSIGSRK ILSNDILHLS SVLNGPLPVY HPQFLIQAIY ANKLQLVKEL 

       790        800        810        820        830        840 
LLRLFLALRK LDFESQDVSN LDSNLGMDPL KYFIAKDRDY PVESFPDPYP CFNKTVSLAL 

       850        860        870        880        890        900 
TEQLTKTTLP YLTRHQQITL ITVIEAVDEV TKNENIVDYN GVRFLLGVKL FLSHKNIQKS 

       910        920        930        940        950        960 
ILMRDVSWAL HSDNKEILLS SIDRHITSWN RAREYRIAYW IKEQDLVKKF EDIAKYEFSK 

       970        980        990       1000       1010       1020 
DDKRDPSRCA IFYLALKKKQ ILLSLWKMAI GHPEQQKMVR FISNDFTVPR WRTAALKNAF 

      1030       1040       1050       1060       1070       1080 
VLLSKHRYMD AAVFFLLTDS LKDCVNVLCK QVHDMDLAIG VCRVYEGDNG PVLGELLTAQ 

      1090       1100       1110       1120       1130       1140 
MLPETIKEND RWKASFIYWK LRKQEVAIKA LLTAPIDLEN NSSIVDKEVC VNRSFLVEDP 

      1150       1160       1170       1180       1190       1200 
ALLYLYNHLR NRNLKYFIGS LNVEAKIECT LILRVTDILC RMGCNYLAVS LVKNWKFIER 

      1210       1220       1230       1240       1250       1260 
NSIPVQKLLK SPTKDRAYSA IGAMASEPIS TARMRPSLFD KFGSPSASDI ESPNPKLPNS 

      1270       1280       1290       1300       1310       1320 
LLDDFLQPPP NSTSSNSLAQ SSSSAPRSIL DEFVSPSYSQ HKENLTPKAP NDSVGETDNS 

      1330       1340       1350 
ENRKDKLSKD ILDDLSSQKP QKPKKSAITK NLLDDFV 

« Hide

References

« Hide 'large scale' references
[1]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Analysis of a 42.5 kb DNA sequence of chromosome X reveals three tRNA genes and 14 new open reading frames including a gene most probably belonging to the family of ubiquitin-protein ligases."
Huang M.-E., Chuat J.-C., Galibert F.
Yeast 11:775-781(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-1016.
Strain: ATCC 204508 / S288c.
[4]"The sequence of 24.3 kb from chromosome X reveals five complete open reading frames, all of which correspond to new genes, and a tandem insertion of a Ty1 transposon."
Zagulski M., Babinska B., Gromadka R., Migdalski A., Rytka J., Sulicka J., Herbert C.J.
Yeast 11:1179-1186(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 728-1357.
[5]"Skp1 forms multiple protein complexes, including RAVE, a regulator of V-ATPase assembly."
Seol J.H., Shevchenko A., Shevchenko A., Deshaies R.J.
Nat. Cell Biol. 3:384-391(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, IDENTIFICATION IN THE RAVE COMPLEX WITH RAV2 AND CBF3D, INTERACTION WITH RAV2; CBF3D; VMA1; VMA2; VMA4 AND VMA8.
[6]"The RAVE complex is essential for stable assembly of the yeast V-ATPase."
Smardon A.M., Tarsio M., Kane P.M.
J. Biol. Chem. 277:13831-13839(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF THE RAVE COMPLEX.
[7]"Soi3p/Rav1p functions at the early endosome to regulate endocytic trafficking to the vacuole and localization of trans-Golgi network transmembrane proteins."
Sipos G., Brickner J.H., Brace E.J., Chen L., Rambourg A., Kepes F., Fuller R.S.
Mol. Biol. Cell 15:3196-3209(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1244, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: YAL6B.
[10]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1244 AND SER-1248, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1244 AND SER-1248, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z49533 Genomic DNA. Translation: CAA89560.1.
L36344 Genomic DNA. Translation: AAA88735.1.
X87297 Genomic DNA. Translation: CAA60726.1.
BK006943 Genomic DNA. Translation: DAA08822.1.
PIRS57052.
RefSeqNP_012567.3. NM_001181691.3.

3D structure databases

ProteinModelPortalP47104.
SMRP47104. Positions 143-173.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33786. 69 interactions.
DIPDIP-916N.
IntActP47104. 6 interactions.
MINTMINT-668932.
STRING4932.YJR033C.

Proteomic databases

MaxQBP47104.
PaxDbP47104.
PeptideAtlasP47104.
PRIDEP47104.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJR033C; YJR033C; YJR033C.
GeneID853490.
KEGGsce:YJR033C.

Organism-specific databases

CYGDYJR033c.
SGDS000003794. RAV1.

Phylogenomic databases

eggNOGNOG314136.
GeneTreeENSGT00390000000096.
HOGENOMHOG000093808.
OMANEYTKTE.
OrthoDBEOG7JHMDP.

Enzyme and pathway databases

BioCycYEAST:G3O-31670-MONOMER.

Gene expression databases

GenevestigatorP47104.

Family and domain databases

Gene3D2.130.10.10. 4 hits.
InterProIPR022033. Rav1p_C.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamPF12234. Rav1p_C. 1 hit.
[Graphical view]
SMARTSM00320. WD40. 5 hits.
[Graphical view]
SUPFAMSSF50978. SSF50978. 4 hits.
ProtoNetSearch...

Other

NextBio974119.

Entry information

Entry nameRAV1_YEAST
AccessionPrimary (citable) accession number: P47104
Secondary accession number(s): D6VWK6
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: June 11, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families