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P47104

- RAV1_YEAST

UniProt

P47104 - RAV1_YEAST

Protein

Regulator of V-ATPase in vacuolar membrane protein 1

Gene

RAV1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Component of the RAVE complex, which is required for stable assembly of the vacuolar ATPase complex V-ATPase under many conditions. Required for transport between the early endosome and the late endosome/prevacuolar compartment (PVC), suggesting that assembly of vacuolar ATPase at the early endosome is required for transport from the early endosome to the PVC.3 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. early endosome to late endosome transport Source: SGD
    2. protein transport Source: UniProtKB-KW
    3. regulation of protein complex assembly Source: SGD
    4. vacuolar acidification Source: SGD

    Keywords - Biological processi

    Protein transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31670-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Regulator of V-ATPase in vacuolar membrane protein 1
    Alternative name(s):
    Suppression of the onset of impotence protein 3
    Gene namesi
    Name:RAV1
    Synonyms:SOI3
    Ordered Locus Names:YJR033C
    ORF Names:J1590
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome X

    Organism-specific databases

    CYGDiYJR033c.
    SGDiS000003794. RAV1.

    Subcellular locationi

    Endomembrane system 1 Publication
    Note: Probably membrane-associated, associates with high density membrane compartment like early endosomes.

    GO - Cellular componenti

    1. cytoplasm Source: SGD
    2. endomembrane system Source: UniProtKB-SubCell
    3. extrinsic component of membrane Source: SGD
    4. RAVE complex Source: SGD

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 13571357Regulator of V-ATPase in vacuolar membrane protein 1PRO_0000051183Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1244 – 12441Phosphoserine3 Publications
    Modified residuei1248 – 12481Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP47104.
    PaxDbiP47104.
    PeptideAtlasiP47104.
    PRIDEiP47104.

    Expressioni

    Gene expression databases

    GenevestigatoriP47104.

    Interactioni

    Subunit structurei

    Component of the RAVE complex composed of RAV1, RAV2 and CBF3D/SKP1. Within the complex, it interacts directly with RAV2 and CBF3D. Interacts with the V-ATPase V1 subunits VMA1, VMA2 and VMA8.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SKP1P522865EBI-25471,EBI-4090
    VMA1P172552EBI-25471,EBI-20245
    VMA2P161404EBI-25471,EBI-20254
    VMA4P222033EBI-25471,EBI-20268
    VMA8P326102EBI-25471,EBI-20264

    Protein-protein interaction databases

    BioGridi33786. 69 interactions.
    DIPiDIP-916N.
    IntActiP47104. 6 interactions.
    MINTiMINT-668932.
    STRINGi4932.YJR033C.

    Structurei

    3D structure databases

    ProteinModelPortaliP47104.
    SMRiP47104. Positions 143-173.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati98 – 13437WD 1Add
    BLAST
    Repeati142 – 18241WD 2Add
    BLAST
    Repeati190 – 23950WD 3Add
    BLAST
    Repeati384 – 42340WD 4Add
    BLAST
    Repeati431 – 47040WD 5Add
    BLAST
    Repeati595 – 63642WD 6Add
    BLAST
    Repeati638 – 67942WD 7Add
    BLAST
    Repeati898 – 93942WD 8Add
    BLAST

    Sequence similaritiesi

    Contains 8 WD repeats.Curated

    Keywords - Domaini

    Repeat, WD repeat

    Phylogenomic databases

    eggNOGiNOG314136.
    GeneTreeiENSGT00390000000096.
    HOGENOMiHOG000093808.
    OMAiNEYTKTE.
    OrthoDBiEOG7JHMDP.

    Family and domain databases

    Gene3Di2.130.10.10. 4 hits.
    InterProiIPR022033. Rav1p_C.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR017986. WD40_repeat_dom.
    [Graphical view]
    PfamiPF12234. Rav1p_C. 1 hit.
    [Graphical view]
    SMARTiSM00320. WD40. 5 hits.
    [Graphical view]
    SUPFAMiSSF50978. SSF50978. 4 hits.

    Sequencei

    Sequence statusi: Complete.

    P47104-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSLNFLPGRP NATPQTACQA TWQNHTIFAY CSGNNLIILT NKFTRLQTIY     50
    TQSDCTAVDI NSQNGFIALS FHNRVLIYKP IHQIMQNPKW TQCCQLFHDD 100
    TPVNCLRWSS DNELAIGSDF LSFWKIKDNF GVYQPILQWN QKQPKPVYNV 150
    IISQDSQLIV SIGKYDCNAK LWKRVSIVGE QAIFNLTMLP HPKPITAMRW 200
    KKEPDQVSKN NTASHALYTL CEDKVLRIWS CFEMEKNHTV QIWGEVPLSP 250
    TQKFCVIIDN WIIRQTLSVK DSEIFDISDS DIVILGSMTG EMEVLALNNL 300
    SQDPPKPMTK KTISHKKVKK ATMLNDTRYL YLPEIQPYDN VKGKLSFLVH 350
    DLQGVIRHLL IDILQLINNK TEDLSAALEH KFTGHNKSVQ KLVRSSDGEA 400
    LLTTSRFSEN GVWYPQKLNH GVSLRLQNTI QTESPIKFAV VHELGKQVIC 450
    LLENGALQAW ECPTNRKEDS EQKQSYLRVE TRLKEEKKIH PIVMLNTPEP 500
    KHSHERHFTA LIFSDGSIKA FEVSLTRGIF EVKSDSLDID GDDIYKISII 550
    DPVHQTFVSN RPLISLITKK GLTRTYKAIV NYNDRHVQWI KACEINTGIM 600
    NCTCIRGSST GKLCIVNSTG KVMSLWDLNR GVLEYEETFH NPIEDIDWTS 650
    TEYGQSIVSI GFTGYALLYT QLRYDYTNNT PSYLPIEKID ITAHTAHNIG 700
    DSVWMKNGTF VVASGNQFYI KDKSLDLTDP FTYQSIGSRK ILSNDILHLS 750
    SVLNGPLPVY HPQFLIQAIY ANKLQLVKEL LLRLFLALRK LDFESQDVSN 800
    LDSNLGMDPL KYFIAKDRDY PVESFPDPYP CFNKTVSLAL TEQLTKTTLP 850
    YLTRHQQITL ITVIEAVDEV TKNENIVDYN GVRFLLGVKL FLSHKNIQKS 900
    ILMRDVSWAL HSDNKEILLS SIDRHITSWN RAREYRIAYW IKEQDLVKKF 950
    EDIAKYEFSK DDKRDPSRCA IFYLALKKKQ ILLSLWKMAI GHPEQQKMVR 1000
    FISNDFTVPR WRTAALKNAF VLLSKHRYMD AAVFFLLTDS LKDCVNVLCK 1050
    QVHDMDLAIG VCRVYEGDNG PVLGELLTAQ MLPETIKEND RWKASFIYWK 1100
    LRKQEVAIKA LLTAPIDLEN NSSIVDKEVC VNRSFLVEDP ALLYLYNHLR 1150
    NRNLKYFIGS LNVEAKIECT LILRVTDILC RMGCNYLAVS LVKNWKFIER 1200
    NSIPVQKLLK SPTKDRAYSA IGAMASEPIS TARMRPSLFD KFGSPSASDI 1250
    ESPNPKLPNS LLDDFLQPPP NSTSSNSLAQ SSSSAPRSIL DEFVSPSYSQ 1300
    HKENLTPKAP NDSVGETDNS ENRKDKLSKD ILDDLSSQKP QKPKKSAITK 1350
    NLLDDFV 1357
    Length:1,357
    Mass (Da):154,933
    Last modified:February 1, 1996 - v1
    Checksum:i6B7270B6901274CF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49533 Genomic DNA. Translation: CAA89560.1.
    L36344 Genomic DNA. Translation: AAA88735.1.
    X87297 Genomic DNA. Translation: CAA60726.1.
    BK006943 Genomic DNA. Translation: DAA08822.1.
    PIRiS57052.
    RefSeqiNP_012567.3. NM_001181691.3.

    Genome annotation databases

    EnsemblFungiiYJR033C; YJR033C; YJR033C.
    GeneIDi853490.
    KEGGisce:YJR033C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49533 Genomic DNA. Translation: CAA89560.1 .
    L36344 Genomic DNA. Translation: AAA88735.1 .
    X87297 Genomic DNA. Translation: CAA60726.1 .
    BK006943 Genomic DNA. Translation: DAA08822.1 .
    PIRi S57052.
    RefSeqi NP_012567.3. NM_001181691.3.

    3D structure databases

    ProteinModelPortali P47104.
    SMRi P47104. Positions 143-173.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33786. 69 interactions.
    DIPi DIP-916N.
    IntActi P47104. 6 interactions.
    MINTi MINT-668932.
    STRINGi 4932.YJR033C.

    Proteomic databases

    MaxQBi P47104.
    PaxDbi P47104.
    PeptideAtlasi P47104.
    PRIDEi P47104.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YJR033C ; YJR033C ; YJR033C .
    GeneIDi 853490.
    KEGGi sce:YJR033C.

    Organism-specific databases

    CYGDi YJR033c.
    SGDi S000003794. RAV1.

    Phylogenomic databases

    eggNOGi NOG314136.
    GeneTreei ENSGT00390000000096.
    HOGENOMi HOG000093808.
    OMAi NEYTKTE.
    OrthoDBi EOG7JHMDP.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31670-MONOMER.

    Miscellaneous databases

    NextBioi 974119.

    Gene expression databases

    Genevestigatori P47104.

    Family and domain databases

    Gene3Di 2.130.10.10. 4 hits.
    InterProi IPR022033. Rav1p_C.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR017986. WD40_repeat_dom.
    [Graphical view ]
    Pfami PF12234. Rav1p_C. 1 hit.
    [Graphical view ]
    SMARTi SM00320. WD40. 5 hits.
    [Graphical view ]
    SUPFAMi SSF50978. SSF50978. 4 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Analysis of a 42.5 kb DNA sequence of chromosome X reveals three tRNA genes and 14 new open reading frames including a gene most probably belonging to the family of ubiquitin-protein ligases."
      Huang M.-E., Chuat J.-C., Galibert F.
      Yeast 11:775-781(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-1016.
      Strain: ATCC 204508 / S288c.
    4. "The sequence of 24.3 kb from chromosome X reveals five complete open reading frames, all of which correspond to new genes, and a tandem insertion of a Ty1 transposon."
      Zagulski M., Babinska B., Gromadka R., Migdalski A., Rytka J., Sulicka J., Herbert C.J.
      Yeast 11:1179-1186(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 728-1357.
    5. "Skp1 forms multiple protein complexes, including RAVE, a regulator of V-ATPase assembly."
      Seol J.H., Shevchenko A., Shevchenko A., Deshaies R.J.
      Nat. Cell Biol. 3:384-391(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION IN THE RAVE COMPLEX WITH RAV2 AND CBF3D, INTERACTION WITH RAV2; CBF3D; VMA1; VMA2; VMA4 AND VMA8.
    6. "The RAVE complex is essential for stable assembly of the yeast V-ATPase."
      Smardon A.M., Tarsio M., Kane P.M.
      J. Biol. Chem. 277:13831-13839(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE RAVE COMPLEX.
    7. "Soi3p/Rav1p functions at the early endosome to regulate endocytic trafficking to the vacuole and localization of trans-Golgi network transmembrane proteins."
      Sipos G., Brickner J.H., Brace E.J., Chen L., Rambourg A., Kepes F., Fuller R.S.
      Mol. Biol. Cell 15:3196-3209(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
      Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
      Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1244, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: YAL6B.
    10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1244 AND SER-1248, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1244 AND SER-1248, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiRAV1_YEAST
    AccessioniPrimary (citable) accession number: P47104
    Secondary accession number(s): D6VWK6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 121 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 149 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3