Reviewed, UniProtKB/Swiss-Prot P47103 (CYP7_YEAST)

Names and origin

Protein names

Recommended name:
    Peptidyl-prolyl cis-trans isomerase CYP7
      Short name=PPIase
      Short name=Rotamase
    EC=5.2.1.8

Gene names

Name:	CPR7
Ordered Locus Names:	YJR032W
ORF Names:	J1585

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	393 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Plays a major role in negative regulation of the heat shock transcription factor (HSF).
Catalytic activity	Peptidylproline (omega=180) = peptidylproline (omega=0).
Subunit structure	Interacts with RPD3 and CNS1.
Miscellaneous	Present with 3230 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Contains 1 PPIase cyclophilin-type domain. Contains 3 TPR repeats.

Ontologies

Keywords
Domain	Repeat TPR repeat
Molecular function	Isomerase Rotamase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein refolding Inferred from direct assay. Source: SGD response to stress Inferred from genetic interaction. Source: SGD
Cellular component	cytosol Inferred from physical interaction. Source: SGD
Molecular function	peptidyl-prolyl cis-trans isomerase activity Inferred from direct assay. Source: SGD unfolded protein binding Inferred from direct assay. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CNS1	P33313	2	EBI-5436,EBI-4806
HSC82	P15108	1	EBI-5436,EBI-8666
HSP82	P02829	1	EBI-5436,EBI-8659
RPD3	P32561	1	EBI-5436,EBI-15864

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 393	393	Peptidyl-prolyl cis-trans isomerase CYP7		PRO_0000064174
Regions
	Domain	8 – 196	189	PPIase cyclophilin-type
	Repeat	240 – 273	34	TPR 1
	Repeat	292 – 325	34	TPR 2
	Repeat	330 – 363	34	TPR 3
Experimental info
	Sequence conflict	237	1	L → F in AAC49415. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P47103-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 1532F255E8016F4F	FASTA	393	45,134
	10 20 30 40 50 60 MIQDPLVYLD ISIDKKPIGR IVCKLFREKA PKTTENFYKL CAGDVKSPLK DQQYLSYKGN 70 80 90 100 110 120 GFHRVVKNFM IQAGDIVFGT QKDSSSSSVG KGGCSIYADK EEVKTDDESF CYGNFEDENL 130 140 150 160 170 180 GEFVEPFTLG MANLGSPNTN NSQFFITTYA APHLNGKHSI FGQVVHGKSV VRTIENCRVD 190 200 210 220 230 240 SDGVPESDVR ISDCGVWEKT MGVPLYNASN DQIGGDVYEE YPDDDTHFGD DDFGKALEAA 250 260 270 280 290 300 NIIKESGTLL FKKKDYSNAF FKYRKSLNYI NEYMPEPDVD KERNIQFINL KMKIYLNLSL 310 320 330 340 350 360 VLFNLERYDD AIMYATYLLE MDNVPNRDQA KAYYRRGNSY LKKKRLDEAL QDYIFCKEKN 370 380 390 PDDEVIEQRI EYVNRLIEEN KEKTRKNISK FFS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of two CyP-40-like cyclophilins in Saccharomyces cerevisiae, one of which is required for normal growth." Duina A.A., Marsh J.A., Gaber R.F. Yeast 12:943-952(1996) [PubMed: 8873448] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The sequence of 24.3 kb from chromosome X reveals five complete open reading frames, all of which correspond to new genes, and a tandem insertion of a Ty1 transposon." Zagulski M., Babinska B., Gromadka R., Migdalski A., Rytka J., Sulicka J., Herbert C.J. Yeast 11:1179-1186(1995) [PubMed: 8619316] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U48868 Genomic DNA. Translation: AAC49415.1. Z49532 Genomic DNA. Translation: CAA89559.1. X87297 Genomic DNA. Translation: CAA60725.1. AY693103 Genomic DNA. Translation: AAT93122.1.
PIR	S57050.
RefSeq	NP_012566.1.
3D structure databases
SMR	P47103. Positions 3-386.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2312N.
IntAct	P47103. 10 interactions.
STRING	P47103.
Proteomic databases
PeptideAtlas	P47103.
Genome annotation databases
Ensembl	YJR032W; YJR032W; YJR032W; Saccharomyces cerevisiae. [Genome view]
GeneID	853489.
KEGG	sce:YJR032W.
NMPDR	fig|4932.3.peg.3540.
Organism-specific databases
CYGD	YJR032w.
SGD	S000003793. CPR7.
Phylogenomic databases
eggNOG	fuNOG05697.
HOGENOM	HBG610621.
OMA	QFFITTY.
OrthoDB	EOG941RVM.
PhylomeDB	P47103.
Enzyme and pathway databases
BRENDA	5.2.1.8. 250.
Gene expression databases
ArrayExpress	P47103.
Genevestigator	P47103.
GermOnline	YJR032W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR015891. Cyclophilin-like. IPR020892. Pep-Pro_Isoase_cyclophilin_CS. IPR002130. PPIase_cyclophilin. IPR013026. TPR-contain. IPR011990. TPR-like_helical. IPR019734. TPR_repeat. [Graphical view]
Gene3D	G3DSA:2.40.100.10. PPIase_cyclophilin. 1 hit. G3DSA:1.25.40.10. TPR-like_helical. 1 hit.
Pfam	PF00160. Pro_isomerase. 1 hit. [Graphical view]
PRINTS	PR00153. CSAPPISMRASE.
SMART	SM00028. TPR. 3 hits. [Graphical view]
PROSITE	PS00170. CSA_PPIASE_1. 1 hit. PS50072. CSA_PPIASE_2. 1 hit. PS50005. TPR. 3 hits. PS50293. TPR_REGION. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	974116.

Entry information

Entry name

CYP7_YEAST

Accession

Primary (citable) accession number: P47103
Secondary accession number(s): Q92323

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	February 9, 2010
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)