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Protein

ARF guanine-nucleotide exchange factor 1

Gene

GEA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Activates the ARF proteins by exchanging bound GDP for free GTP.

GO - Molecular functioni

  • ARF guanyl-nucleotide exchange factor activity Source: SGD

GO - Biological processi

  • actin cytoskeleton organization Source: SGD
  • ER to Golgi vesicle-mediated transport Source: SGD
  • intra-Golgi vesicle-mediated transport Source: SGD
  • macroautophagy Source: SGD
  • regulation of ARF protein signal transduction Source: InterPro
  • retrograde vesicle-mediated transport, Golgi to ER Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Enzyme and pathway databases

BioCyciYEAST:G3O-31669-MONOMER.
ReactomeiR-SCE-421837. Clathrin derived vesicle budding.
R-SCE-5620916. VxPx cargo-targeting to cilium.
R-SCE-6807878. COPI-mediated anterograde transport.
R-SCE-6811434. COPI-dependent Golgi-to-ER retrograde traffic.

Names & Taxonomyi

Protein namesi
Recommended name:
ARF guanine-nucleotide exchange factor 1
Gene namesi
Name:GEA1
Ordered Locus Names:YJR031C
ORF Names:J1580
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJR031C.
SGDiS000003792. GEA1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB-SubCell
  • extrinsic component of membrane Source: SGD
  • Golgi-associated vesicle Source: SGD
  • Golgi cis cisterna Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi862L → S: Abolishes interaction with GMH1. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001202121 – 1408ARF guanine-nucleotide exchange factor 1Add BLAST1408

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei49PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP47102.
PRIDEiP47102.

Interactioni

Subunit structurei

Interacts with GMH1. Interacts (via N-terminal region) with SEC21 (via C-terminus).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SEC21P320746EBI-7539,EBI-4891

Protein-protein interaction databases

BioGridi33784. 31 interactors.
DIPiDIP-5697N.
IntActiP47102. 7 interactors.
MINTiMINT-532353.

Structurei

Secondary structure

11408
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi540 – 554Combined sources15
Helixi556 – 565Combined sources10
Beta strandi568 – 570Combined sources3
Helixi574 – 584Combined sources11
Helixi585 – 587Combined sources3
Helixi590 – 597Combined sources8
Helixi600 – 602Combined sources3
Helixi603 – 610Combined sources8
Helixi620 – 627Combined sources8
Helixi637 – 653Combined sources17
Helixi687 – 705Combined sources19
Helixi715 – 721Combined sources7
Turni722 – 725Combined sources4
Helixi733 – 745Combined sources13
Helixi751 – 753Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1RE0X-ray2.40B540-754[»]
ProteinModelPortaliP47102.
SMRiP47102.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP47102.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini552 – 706SEC7PROSITE-ProRule annotationAdd BLAST155

Domaini

The SEC7 domain is sufficient for stimulation of nucleotide exchange on myristoylated yeast ARF2.

Sequence similaritiesi

Contains 1 SEC7 domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00860000133718.
HOGENOMiHOG000248822.
InParanoidiP47102.
KOiK18443.
OMAiITVKLFT.
OrthoDBiEOG092C066Z.

Family and domain databases

Gene3Di1.10.1000.11. 1 hit.
InterProiIPR023394. Sec7_alpha_orthog.
IPR000904. Sec7_dom.
IPR032691. Sec7_N.
[Graphical view]
PfamiPF01369. Sec7. 1 hit.
PF12783. Sec7_N. 1 hit.
[Graphical view]
SMARTiSM00222. Sec7. 1 hit.
[Graphical view]
SUPFAMiSSF48425. SSF48425. 1 hit.
PROSITEiPS50190. SEC7. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47102-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHDVPMETVL AVNPATMIVK ECINLCSAMN KQSRDKSQTS VAALLGGGSD
60 70 80 90 100
IFLSQSDSFV DSFHNLPTSS YHDPLISGLV QLRLKINDLK GLDSLNALEL
110 120 130 140 150
LKPFLEIVSA SSVSGYTTSL ALDSLQKVFT LKIINKTFND IQIAVRETVV
160 170 180 190 200
ALTHCRFEAS KQISDDSVLL KVVTLLRDII TSSFGDYLSD TIIYDVLQTT
210 220 230 240 250
LSLACNTQRS EVLRKTAEVT IAGITVKLFT KLKLLDPPTK TEKYINDESY
260 270 280 290 300
TDNNLKDDII GTTTSDNDLS STDDDSAVAD DNKNEKPVQQ VIREQENDEE
310 320 330 340 350
TAEKAENVEP NYGITVIKDY LGLLLSLVMP ENRMKHTTSA MKLSLQLINA
360 370 380 390 400
AIEISGDKFP LYPRLFSLIS DPIFKSVLFI IQSSTQYSLL QATLQLFTSL
410 420 430 440 450
VVILGDYLPM QIELTLRRIF EILEDTTISG DVSKQKPPAI RELIIEQLSI
460 470 480 490 500
LWIHSPAFFL QLFVNFDCNL DRSDLSIDFI KELTKFSLPA AAVNTSNNIP
510 520 530 540 550
PICLEGVLSL IENIYNDLQR FDRAEFVKNQ KEIDILKQRD RKTEFILCVE
560 570 580 590 600
TFNEKAKKGI QMLIEKGFID SDSNRDIASF LFLNNGRLNK KTIGLLLCDP
610 620 630 640 650
KKTSLLKEFI DLFDFKGLRV DEAIRILLTK FRLPGESQQI ERIVEAFSSK
660 670 680 690 700
YSADQSNDKV ELEDKKAGKN GSESMTEDDI IHVQPDADSV FVLSYSIIML
710 720 730 740 750
NTDSHNPQVK DHMTFDDYSN NLRGCYNGKD FPRWYLHKIY TSIKVKEIVM
760 770 780 790 800
PEEHHGNERW FEDAWNNLIS STSVMTEMQR DFTNPISKLA QIDILQYEKA
810 820 830 840 850
IFSNVRDIIL KTLFKIFTVA SSDQISLRIL DAISKCTFIN YYFSFDQSYN
860 870 880 890 900
DTVLHLGEMT TLAQSSAKAV ELDVDSIPLV EIFVEDTGSK ISVSNQSIRL
910 920 930 940 950
GQNFKAQLCT VLYFQIIKEI SDPSIVSTRL WNQIVQLILK LFENLLMEPN
960 970 980 990 1000
LPFFTNFHSL LKLPELPLPD PDISIRKAKM SRSLLSTFAS YLKGDEEPSE
1010 1020 1030 1040 1050
EDIDFSIKAF ECVKASHPLS SVFENNQLVS PKMIETLLSS LVIEKTSENS
1060 1070 1080 1090 1100
PYFEQELLFL LEISIILISE ASYGQEFGAL IADHMINISN LDGLSKEAIA
1110 1120 1130 1140 1150
RLASYKMFLV SRFDNPRDIL SDLIEHDFLV KNEIFNTKYY ESEWGKQVIN
1160 1170 1180 1190 1200
DLFTHLNDVK YNERALKNVK FWNFLRILIS AKDRQFAVYT FLEKYIQNGD
1210 1220 1230 1240 1250
IFVDDGNFMN ILSLLDEMSC AGAVGTKWEQ NYENSVEDGC EAPESNPYRS
1260 1270 1280 1290 1300
IIDLSSRSIN ITADLLSTVG RSNSALNKNE IIAAIQGLAH QCLNPCDELG
1310 1320 1330 1340 1350
MQALQALENI LLSRASQLRT EKVAVDNLLE TGLLPIFELD EIQDVKMKRI
1360 1370 1380 1390 1400
TSILSVLSKI FLGQLVEGVT SNETFLRVLN VFNKYVDDPT VERQLQELII

SKREIEKE
Length:1,408
Mass (Da):159,437
Last modified:February 1, 1996 - v1
Checksum:i3385C8FA4252EBBE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49531 Genomic DNA. Translation: CAA89558.1.
X87297 Genomic DNA. Translation: CAA60724.1.
BK006943 Genomic DNA. Translation: DAA08820.1.
PIRiS57049.
RefSeqiNP_012565.1. NM_001181689.1.

Genome annotation databases

EnsemblFungiiYJR031C; YJR031C; YJR031C.
GeneIDi853488.
KEGGisce:YJR031C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49531 Genomic DNA. Translation: CAA89558.1.
X87297 Genomic DNA. Translation: CAA60724.1.
BK006943 Genomic DNA. Translation: DAA08820.1.
PIRiS57049.
RefSeqiNP_012565.1. NM_001181689.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1RE0X-ray2.40B540-754[»]
ProteinModelPortaliP47102.
SMRiP47102.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33784. 31 interactors.
DIPiDIP-5697N.
IntActiP47102. 7 interactors.
MINTiMINT-532353.

Proteomic databases

MaxQBiP47102.
PRIDEiP47102.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJR031C; YJR031C; YJR031C.
GeneIDi853488.
KEGGisce:YJR031C.

Organism-specific databases

EuPathDBiFungiDB:YJR031C.
SGDiS000003792. GEA1.

Phylogenomic databases

GeneTreeiENSGT00860000133718.
HOGENOMiHOG000248822.
InParanoidiP47102.
KOiK18443.
OMAiITVKLFT.
OrthoDBiEOG092C066Z.

Enzyme and pathway databases

BioCyciYEAST:G3O-31669-MONOMER.
ReactomeiR-SCE-421837. Clathrin derived vesicle budding.
R-SCE-5620916. VxPx cargo-targeting to cilium.
R-SCE-6807878. COPI-mediated anterograde transport.
R-SCE-6811434. COPI-dependent Golgi-to-ER retrograde traffic.

Miscellaneous databases

EvolutionaryTraceiP47102.
PROiP47102.

Family and domain databases

Gene3Di1.10.1000.11. 1 hit.
InterProiIPR023394. Sec7_alpha_orthog.
IPR000904. Sec7_dom.
IPR032691. Sec7_N.
[Graphical view]
PfamiPF01369. Sec7. 1 hit.
PF12783. Sec7_N. 1 hit.
[Graphical view]
SMARTiSM00222. Sec7. 1 hit.
[Graphical view]
SUPFAMiSSF48425. SSF48425. 1 hit.
PROSITEiPS50190. SEC7. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGEA1_YEAST
AccessioniPrimary (citable) accession number: P47102
Secondary accession number(s): D6VWK4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 30, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2940 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.