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P47096

- 3HAO_YEAST

UniProt

P47096 - 3HAO_YEAST

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Protein
3-hydroxyanthranilate 3,4-dioxygenase
Gene
BNA1, HAD1, YJR025C, J1550
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the oxidative ring opening of 3-hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde, which spontaneously cyclizes to quinolinate.UniRule annotation

Catalytic activityi

3-hydroxyanthranilate + O2 = 2-amino-3-carboxymuconate semialdehyde.1 Publication

Cofactori

Fe2+ ion.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei45 – 451Dioxygen By similarity
Metal bindingi49 – 491Iron; catalytic
Metal bindingi55 – 551Iron; catalytic
Binding sitei55 – 551Substrate By similarity
Metal bindingi97 – 971Iron; catalytic
Binding sitei101 – 1011Substrate By similarity
Binding sitei111 – 1111Substrate By similarity
Metal bindingi126 – 1261Divalent metal cation
Metal bindingi129 – 1291Divalent metal cation
Metal bindingi163 – 1631Divalent metal cation
Metal bindingi166 – 1661Divalent metal cation

GO - Molecular functioni

  1. 3-hydroxyanthranilate 3,4-dioxygenase activity Source: SGD
  2. ferrous iron binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. 'de novo' NAD biosynthetic process from tryptophan Source: SGD
  2. anthranilate metabolic process Source: UniProtKB-HAMAP
  3. quinolinate biosynthetic process Source: UniProtKB-HAMAP
  4. tryptophan catabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Pyridine nucleotide biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-8161.
YEAST:YJR025C-MONOMER.
BRENDAi1.13.11.6. 984.
UniPathwayiUPA00253; UER00330.

Names & Taxonomyi

Protein namesi
Recommended name:
3-hydroxyanthranilate 3,4-dioxygenase (EC:1.13.11.6)
Alternative name(s):
3-hydroxyanthranilate oxygenase
Short name:
3-HAO
3-hydroxyanthranilic acid dioxygenase
Short name:
HAD
Biosynthesis of nicotinic acid protein 1
Gene namesi
Name:BNA1
Synonyms:HAD1
Ordered Locus Names:YJR025C
ORF Names:J1550
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

CYGDiYJR025c.
SGDiS000003786. BNA1.

Subcellular locationi

Cytoplasm. Nucleus 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: SGD
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 1771773-hydroxyanthranilate 3,4-dioxygenaseUniRule annotation
PRO_0000064375Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei176 – 1761Phosphoserine3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP47096.
PaxDbiP47096.
PeptideAtlasiP47096.

Expressioni

Gene expression databases

GenevestigatoriP47096.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi33778. 47 interactions.
DIPiDIP-4759N.
IntActiP47096. 1 interaction.
MINTiMINT-529506.
STRINGi4932.YJR025C.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi9 – 168
Helixi17 – 204
Beta strandi21 – 244
Beta strandi26 – 305
Beta strandi32 – 398
Beta strandi41 – 433
Beta strandi48 – 503
Beta strandi55 – 628
Beta strandi64 – 707
Beta strandi72 – 754
Beta strandi77 – 837
Beta strandi87 – 915
Beta strandi97 – 1015
Beta strandi106 – 1127
Beta strandi116 – 1183
Beta strandi121 – 1255
Turni127 – 1293
Beta strandi132 – 1376
Beta strandi140 – 1423
Helixi145 – 15511
Helixi158 – 1614
Turni164 – 1663

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZVFX-ray2.41A/B1-175[»]
ProteinModelPortaliP47096.
SMRiP47096. Positions 1-175.

Miscellaneous databases

EvolutionaryTraceiP47096.

Family & Domainsi

Sequence similaritiesi

Belongs to the 3-HAO family.

Phylogenomic databases

eggNOGiNOG77058.
GeneTreeiENSGT00390000013008.
HOGENOMiHOG000218448.
KOiK00452.
OMAiHINQTPE.
OrthoDBiEOG7QK0Q0.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
HAMAPiMF_00825. 3_HAO.
InterProiIPR010329. 3hydroanth_dOase.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR15497. PTHR15497. 1 hit.
PfamiPF06052. 3-HAO. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.
TIGRFAMsiTIGR03037. anthran_nbaC. 1 hit.

Sequencei

Sequence statusi: Complete.

P47096-1 [UniParc]FASTAAdd to Basket

« Hide

MFNTTPINID KWLKENEGLL KPPVNNYCLH KGGFTVMIVG GPNERTGYHI    50
NPTPEWFYQK KGSMLLKVVD ETDAEPKFID IIINEGDSYL LPGNVPHSPV 100
RFADTVGIVV EQDRPGGEND KIRWYCSHCR QVVHESELQM LDLGTQVKEA 150
ILDFENDVEK RTCFHCKTLN YARPQSN 177
Length:177
Mass (Da):20,235
Last modified:February 1, 1996 - v1
Checksum:i930D69F486632417
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z49525 Genomic DNA. Translation: CAA89550.1.
X87297 Genomic DNA. Translation: CAA60720.1.
AY558309 Genomic DNA. Translation: AAS56635.1.
BK006943 Genomic DNA. Translation: DAA08814.1.
PIRiS57043.
RefSeqiNP_012559.1. NM_001181683.1.

Genome annotation databases

EnsemblFungiiYJR025C; YJR025C; YJR025C.
GeneIDi853482.
KEGGisce:YJR025C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z49525 Genomic DNA. Translation: CAA89550.1 .
X87297 Genomic DNA. Translation: CAA60720.1 .
AY558309 Genomic DNA. Translation: AAS56635.1 .
BK006943 Genomic DNA. Translation: DAA08814.1 .
PIRi S57043.
RefSeqi NP_012559.1. NM_001181683.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1ZVF X-ray 2.41 A/B 1-175 [» ]
ProteinModelPortali P47096.
SMRi P47096. Positions 1-175.
ModBasei Search...

Protein-protein interaction databases

BioGridi 33778. 47 interactions.
DIPi DIP-4759N.
IntActi P47096. 1 interaction.
MINTi MINT-529506.
STRINGi 4932.YJR025C.

Proteomic databases

MaxQBi P47096.
PaxDbi P47096.
PeptideAtlasi P47096.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YJR025C ; YJR025C ; YJR025C .
GeneIDi 853482.
KEGGi sce:YJR025C.

Organism-specific databases

CYGDi YJR025c.
SGDi S000003786. BNA1.

Phylogenomic databases

eggNOGi NOG77058.
GeneTreei ENSGT00390000013008.
HOGENOMi HOG000218448.
KOi K00452.
OMAi HINQTPE.
OrthoDBi EOG7QK0Q0.

Enzyme and pathway databases

UniPathwayi UPA00253 ; UER00330 .
BioCyci MetaCyc:MONOMER-8161.
YEAST:YJR025C-MONOMER.
BRENDAi 1.13.11.6. 984.

Miscellaneous databases

EvolutionaryTracei P47096.
NextBioi 974095.
PROi P47096.

Gene expression databases

Genevestigatori P47096.

Family and domain databases

Gene3Di 2.60.120.10. 1 hit.
HAMAPi MF_00825. 3_HAO.
InterProi IPR010329. 3hydroanth_dOase.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view ]
PANTHERi PTHR15497. PTHR15497. 1 hit.
Pfami PF06052. 3-HAO. 1 hit.
[Graphical view ]
SUPFAMi SSF51182. SSF51182. 1 hit.
TIGRFAMsi TIGR03037. anthran_nbaC. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The sequence of 24.3 kb from chromosome X reveals five complete open reading frames, all of which correspond to new genes, and a tandem insertion of a Ty1 transposon."
    Zagulski M., Babinska B., Gromadka R., Migdalski A., Rytka J., Sulicka J., Herbert C.J.
    Yeast 11:1179-1186(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "The yeast gene YJR025c encodes a 3-hydroxyanthranilic acid dioxygenase and is involved in nicotinic acid biosynthesis."
    Kucharczyk R., Zagulski M., Rytka J., Herbert C.J.
    FEBS Lett. 424:127-130(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, PATHWAY.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-176, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-176, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-176, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Crystal structure of 3-hydroxyanthranilic acid 3,4-dioxygenase from Saccharomyces cerevisiae: a special subgroup of the type III extradiol dioxygenases."
    Li X., Guo M., Fan J., Tang W., Wang D., Ge H., Rong H., Teng M., Niu L., Liu Q., Hao Q.
    Protein Sci. 15:761-773(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS) OF 1-175 IN COMPLEX WITH DIVALENT METAL IONS, SUBUNIT.

Entry informationi

Entry namei3HAO_YEAST
AccessioniPrimary (citable) accession number: P47096
Secondary accession number(s): D6VWJ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: June 11, 2014
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2330 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3

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