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Protein

3-hydroxyanthranilate 3,4-dioxygenase

Gene

BNA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the oxidative ring opening of 3-hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde, which spontaneously cyclizes to quinolinate.

Catalytic activityi

3-hydroxyanthranilate + O2 = 2-amino-3-carboxymuconate semialdehyde.UniRule annotation1 Publication

Cofactori

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes quinolinate from L-kynurenine.UniRule annotation1 Publication
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Kynurenine 3-monooxygenase (BNA4)
  2. Kynureninase (BNA5)
  3. 3-hydroxyanthranilate 3,4-dioxygenase (BNA1)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes quinolinate from L-kynurenine, the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei45DioxygenUniRule annotation1
Metal bindingi49Iron; catalytic1
Metal bindingi55Iron; catalytic1
Binding sitei55SubstrateUniRule annotation1
Metal bindingi97Iron; catalytic1
Binding sitei101SubstrateUniRule annotation1
Binding sitei111SubstrateUniRule annotation1
Metal bindingi126Divalent metal cation1
Metal bindingi129Divalent metal cation1
Metal bindingi163Divalent metal cation1
Metal bindingi166Divalent metal cation1

GO - Molecular functioni

  • 3-hydroxyanthranilate 3,4-dioxygenase activity Source: SGD
  • ferrous iron binding Source: UniProtKB-HAMAP

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Pyridine nucleotide biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:YJR025C-MONOMER.
YEAST:YJR025C-MONOMER.
BRENDAi1.13.11.6. 984.
ReactomeiR-SCE-71240. Tryptophan catabolism.
UniPathwayiUPA00253; UER00330.

Names & Taxonomyi

Protein namesi
Recommended name:
3-hydroxyanthranilate 3,4-dioxygenaseUniRule annotation (EC:1.13.11.6UniRule annotation)
Alternative name(s):
3-hydroxyanthranilate oxygenaseUniRule annotation
Short name:
3-HAOUniRule annotation
3-hydroxyanthranilic acid dioxygenaseUniRule annotation
Short name:
HADUniRule annotation
Biosynthesis of nicotinic acid protein 1UniRule annotation
Gene namesi
Name:BNA1UniRule annotation
Synonyms:HAD1
Ordered Locus Names:YJR025C
ORF Names:J1550
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJR025C.
SGDiS000003786. BNA1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000643751 – 1773-hydroxyanthranilate 3,4-dioxygenaseAdd BLAST177

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei176PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP47096.
PRIDEiP47096.

PTM databases

iPTMnetiP47096.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi33778. 48 interactors.
DIPiDIP-4759N.
IntActiP47096. 1 interactor.
MINTiMINT-529506.

Structurei

Secondary structure

1177
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi9 – 16Combined sources8
Helixi17 – 20Combined sources4
Beta strandi21 – 24Combined sources4
Beta strandi26 – 30Combined sources5
Beta strandi32 – 39Combined sources8
Beta strandi41 – 43Combined sources3
Beta strandi48 – 50Combined sources3
Beta strandi55 – 62Combined sources8
Beta strandi64 – 70Combined sources7
Beta strandi72 – 75Combined sources4
Beta strandi77 – 83Combined sources7
Beta strandi87 – 91Combined sources5
Beta strandi97 – 101Combined sources5
Beta strandi106 – 112Combined sources7
Beta strandi116 – 118Combined sources3
Beta strandi121 – 125Combined sources5
Turni127 – 129Combined sources3
Beta strandi132 – 137Combined sources6
Beta strandi140 – 142Combined sources3
Helixi145 – 155Combined sources11
Helixi158 – 161Combined sources4
Turni164 – 166Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZVFX-ray2.41A/B1-175[»]
ProteinModelPortaliP47096.
SMRiP47096.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP47096.

Family & Domainsi

Sequence similaritiesi

Belongs to the 3-HAO family.UniRule annotation

Phylogenomic databases

GeneTreeiENSGT00390000013008.
HOGENOMiHOG000218448.
InParanoidiP47096.
KOiK00452.
OMAiKPPVGNQ.
OrthoDBiEOG092C5CUF.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
HAMAPiMF_00825. 3_HAO. 1 hit.
InterProiIPR010329. 3hydroanth_dOase.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR15497. PTHR15497. 1 hit.
PfamiPF06052. 3-HAO. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.
TIGRFAMsiTIGR03037. anthran_nbaC. 1 hit.

Sequencei

Sequence statusi: Complete.

P47096-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFNTTPINID KWLKENEGLL KPPVNNYCLH KGGFTVMIVG GPNERTGYHI
60 70 80 90 100
NPTPEWFYQK KGSMLLKVVD ETDAEPKFID IIINEGDSYL LPGNVPHSPV
110 120 130 140 150
RFADTVGIVV EQDRPGGEND KIRWYCSHCR QVVHESELQM LDLGTQVKEA
160 170
ILDFENDVEK RTCFHCKTLN YARPQSN
Length:177
Mass (Da):20,235
Last modified:February 1, 1996 - v1
Checksum:i930D69F486632417
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49525 Genomic DNA. Translation: CAA89550.1.
X87297 Genomic DNA. Translation: CAA60720.1.
AY558309 Genomic DNA. Translation: AAS56635.1.
BK006943 Genomic DNA. Translation: DAA08814.1.
PIRiS57043.
RefSeqiNP_012559.1. NM_001181683.1.

Genome annotation databases

EnsemblFungiiYJR025C; YJR025C; YJR025C.
GeneIDi853482.
KEGGisce:YJR025C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49525 Genomic DNA. Translation: CAA89550.1.
X87297 Genomic DNA. Translation: CAA60720.1.
AY558309 Genomic DNA. Translation: AAS56635.1.
BK006943 Genomic DNA. Translation: DAA08814.1.
PIRiS57043.
RefSeqiNP_012559.1. NM_001181683.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZVFX-ray2.41A/B1-175[»]
ProteinModelPortaliP47096.
SMRiP47096.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33778. 48 interactors.
DIPiDIP-4759N.
IntActiP47096. 1 interactor.
MINTiMINT-529506.

PTM databases

iPTMnetiP47096.

Proteomic databases

MaxQBiP47096.
PRIDEiP47096.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJR025C; YJR025C; YJR025C.
GeneIDi853482.
KEGGisce:YJR025C.

Organism-specific databases

EuPathDBiFungiDB:YJR025C.
SGDiS000003786. BNA1.

Phylogenomic databases

GeneTreeiENSGT00390000013008.
HOGENOMiHOG000218448.
InParanoidiP47096.
KOiK00452.
OMAiKPPVGNQ.
OrthoDBiEOG092C5CUF.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00330.
BioCyciMetaCyc:YJR025C-MONOMER.
YEAST:YJR025C-MONOMER.
BRENDAi1.13.11.6. 984.
ReactomeiR-SCE-71240. Tryptophan catabolism.

Miscellaneous databases

EvolutionaryTraceiP47096.
PROiP47096.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
HAMAPiMF_00825. 3_HAO. 1 hit.
InterProiIPR010329. 3hydroanth_dOase.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR15497. PTHR15497. 1 hit.
PfamiPF06052. 3-HAO. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.
TIGRFAMsiTIGR03037. anthran_nbaC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry namei3HAO_YEAST
AccessioniPrimary (citable) accession number: P47096
Secondary accession number(s): D6VWJ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 2, 2016
This is version 145 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2330 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.