P47090 (YJY5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uncharacterized endoplasmic reticulum membrane protein YJR015W

Gene names

Ordered Locus Names:	YJR015W
ORF Names:	J1448

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	510 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.4.
Miscellaneous	Present with 639 molecules/cell in log phase SD medium.
Sequence similarities	To yeast SNG1.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	endoplasmic reticulum Inferred from direct assay Ref.4. Source: SGD endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 510	510	Uncharacterized endoplasmic reticulum membrane protein YJR015W		PRO_0000203085
Regions
	Topological domain	1 – 89	89	Lumenal Potential
	Transmembrane	90 – 110	21	Helical; Potential
	Topological domain	111 – 123	13	Cytoplasmic Potential
	Transmembrane	124 – 144	21	Helical; Potential
	Topological domain	145 – 312	168	Lumenal Potential
	Transmembrane	313 – 333	21	Helical; Potential
	Topological domain	334 – 349	16	Cytoplasmic Potential
	Transmembrane	350 – 370	21	Helical; Potential
	Topological domain	371 – 381	11	Lumenal Potential
	Transmembrane	382 – 402	21	Helical; Potential
	Topological domain	403 – 416	14	Cytoplasmic Potential
	Transmembrane	417 – 437	21	Helical; Potential
	Topological domain	438 – 474	37	Lumenal Potential
	Transmembrane	475 – 495	21	Helical; Potential
	Topological domain	496 – 510	15	Cytoplasmic Potential
Experimental info
	Sequence conflict	324	1	F → S in AAU09753. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P47090 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: CD1AB973D0042CBF	FASTA	510	58,117
	10 20 30 40 50 60 MTSSLDDIEP TAYNNMEADE EYCRRNDIHD LSSVVGDAVS QGVPDMDGQT TDSSKDPEPN 70 80 90 100 110 120 SEDKKAFPPS SGSFFSPNLQ GQRKKVLLKF VFTNCLLAII CFTMFVLFWG ALYDTSKYLH 130 140 150 160 170 180 KVKLLVVIQE PPVVILDNNS SMVVPSISYA LPTFINKIPC DWDIYNSPTF QAKFDVNTPQ 190 200 210 220 230 240 QVNDKVVDLV YDEKYWFAIN IKPNATETLF ESLINDTAPL FNSTLFNQVV YETGRDPTNL 250 260 270 280 290 300 KSTILPVAQT IEEYYHTFYT LNYLPPLLTN ITQVYRYALT NNARYIAAAG KYNYEYYDHR 310 320 330 340 350 360 PFTDRILLAP TQIGVVYCLL LTFFQFLLYG PLHVEMAKVL RPANGLIYRI AMSWFTFFFA 370 380 390 400 410 420 SLFFCTTTAI FQVDFTKSFG RGGFVVYWMS TWLFMLAAGG ANENAVMLVI TLGPQYLGFW 430 440 450 460 470 480 ILSFVILNIA PSFFPLALNN NVYRYGYMMP VHNVIDIYRV IFFDVTRRKM GRNYGILVAL 490 500 510 IALNTALLPF VGKYASRKLK QKALVAAKQS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X87611 Genomic DNA. Translation: CAA60938.1. Z49515 Genomic DNA. Translation: CAA89539.1. AY723836 Genomic DNA. Translation: AAU09753.1. BK006943 Genomic DNA. Translation: DAA08807.1.
PIR	S55204.
RefSeq	NP_012549.3. NM_001181673.3.
3D structure databases
ProteinModelPortal	P47090.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1889N.
IntAct	P47090. 4 interactions.
MINT	MINT-402324.
STRING	4932.YJR015W.
Proteomic databases
PaxDb	P47090.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJR015W; YJR015W; YJR015W.
GeneID	853472.
KEGG	sce:YJR015W. sce:YJR019C.
Organism-specific databases
CYGD	YJR015w.
SGD	S000003776. YJR015W.
Phylogenomic databases
eggNOG	NOG42045.
GeneTree	ENSGT00390000015455.
HOGENOM	HOG000141871.
KO	K01068.
OMA	WAVQFRS.
OrthoDB	EOG4TF3V7.
Gene expression databases
Genevestigator	P47090.
GermOnline	YJR015W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR022703. DUF3533. [Graphical view]
Pfam	PF12051. DUF3533. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	974074.

Entry information

Entry name

YJY5_YEAST

Accession

Primary (citable) accession number: P47090
Secondary accession number(s): D6VWJ1, Q66R65

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	April 3, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program