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Protein

GPI mannosyltransferase 1

Gene

GPI14

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers the first alpha-1,4-mannose to GlcN-acyl-PI during GPI precursor assembly. Required for cell wall integrity.1 Publication

Pathwayi

GO - Molecular functioni

  1. transferase activity, transferring hexosyl groups Source: InterPro

GO - Biological processi

  1. fungal-type cell wall organization Source: SGD
  2. GPI anchor biosynthetic process Source: SGD
  3. mannosylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Cell wall biogenesis/degradation, GPI-anchor biosynthesis

Enzyme and pathway databases

BioCyciYEAST:G3O-31658-MONOMER.
UniPathwayiUPA00196.

Protein family/group databases

CAZyiGT50. Glycosyltransferase Family 50.

Names & Taxonomyi

Protein namesi
Recommended name:
GPI mannosyltransferase 1 (EC:2.4.1.-)
Alternative name(s):
GPI mannosyltransferase I
Short name:
GPI-MT-I
Glycosylphosphatidylinositol-anchor biosynthesis protein 14
Gene namesi
Name:GPI14
Synonyms:PMH1
Ordered Locus Names:YJR013W
ORF Names:J1444, YJR83.11
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome X

Organism-specific databases

CYGDiYJR013w.
SGDiS000003774. GPI14.

Subcellular locationi

Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 44CytoplasmicSequence Analysis
Transmembranei5 – 2521HelicalSequence AnalysisAdd
BLAST
Topological domaini26 – 7853LumenalSequence AnalysisAdd
BLAST
Transmembranei79 – 9921HelicalSequence AnalysisAdd
BLAST
Topological domaini100 – 11011CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei111 – 13121HelicalSequence AnalysisAdd
BLAST
Topological domaini132 – 1321LumenalSequence Analysis
Transmembranei133 – 14917HelicalSequence AnalysisAdd
BLAST
Topological domaini150 – 16011CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei161 – 18121HelicalSequence AnalysisAdd
BLAST
Topological domaini182 – 19312LumenalSequence AnalysisAdd
BLAST
Transmembranei194 – 21421HelicalSequence AnalysisAdd
BLAST
Topological domaini215 – 26652CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei267 – 28721HelicalSequence AnalysisAdd
BLAST
Topological domaini288 – 31023LumenalSequence AnalysisAdd
BLAST
Transmembranei311 – 33121HelicalSequence AnalysisAdd
BLAST
Topological domaini332 – 3343CytoplasmicSequence Analysis
Transmembranei335 – 35521HelicalSequence AnalysisAdd
BLAST
Topological domaini356 – 3616LumenalSequence Analysis
Transmembranei362 – 38221HelicalSequence AnalysisAdd
BLAST
Topological domaini383 – 40321CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: SGD
  2. glycosylphosphatidylinositol-mannosyltransferase I complex Source: SGD
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 403403GPI mannosyltransferase 1PRO_0000203084Add
BLAST

Proteomic databases

MaxQBiP47088.
PaxDbiP47088.

Expressioni

Gene expression databases

GenevestigatoriP47088.

Interactioni

Protein-protein interaction databases

BioGridi33769. 11 interactions.
IntActiP47088. 54 interactions.
MINTiMINT-4491536.
STRINGi4932.YJR013W.

Family & Domainsi

Sequence similaritiesi

Belongs to the PIGM family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG268708.
GeneTreeiENSGT00390000017728.
HOGENOMiHOG000186884.
InParanoidiP47088.
KOiK05284.
OMAiEFLEHTY.
OrthoDBiEOG77DJGT.

Family and domain databases

InterProiIPR007704. Mannosyltransferase_DXD.
[Graphical view]
PANTHERiPTHR12886. PTHR12886. 1 hit.
PfamiPF05007. Mannosyl_trans. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47088-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTGEEWGLTV LSFLVRVGFF LFGIYQDANF KVRYTDIDYF VFHDAAKYVY
60 70 80 90 100
EGKSPYARDT YRYTPLLSWL LVPNHYFGWF HLGKVIFVIF DLVTGLIIMK
110 120 130 140 150
LLNQAISRKR ALILESIWLL NPMVITISTR GNAESVLCCL IMFTLFFLQK
160 170 180 190 200
SRYTLAGILY GLSIHFKIYP IIYCIPIAIF IYYNKRNQGP RTQLTSLLNI
210 220 230 240 250
GLSTLTTLLG CGWAMYKIYG YEFLDQAYLY HLYRTDHRHN FSVWNMLLYL
260 270 280 290 300
DSANKENGES NLSRYAFVPQ LLLVLVTGCL EWWNPTFDNL LRVLFVQTFA
310 320 330 340 350
FVTYNKVCTS QYFVWYLIFL PFYLSRTHIG WKKGLLMATL WVGTQGIWLS
360 370 380 390 400
QGYYLEFEGK NVFYPGLFIA SVLFFVTNVW LLGQFITDIK IPTQPTVSNK

KNN
Length:403
Mass (Da):47,166
Last modified:July 24, 2006 - v2
Checksum:iD1017701557FDB0F
GO

Sequence cautioni

The sequence CAA60935.1 differs from that shown. Reason: Frameshift at position 40. Curated
The sequence CAA89537.1 differs from that shown. Reason: Frameshift at position 40. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87611 Genomic DNA. Translation: CAA60935.1. Frameshift.
Z49513 Genomic DNA. Translation: CAA89537.1. Frameshift.
AY260895 Genomic DNA. Translation: AAP21763.1.
AY557910 Genomic DNA. Translation: AAS56236.1.
BK006943 Genomic DNA. Translation: DAA08805.1.
PIRiS55201.
RefSeqiNP_012547.2. NM_001181671.1.

Genome annotation databases

EnsemblFungiiYJR013W; YJR013W; YJR013W.
GeneIDi853470.
KEGGisce:YJR013W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87611 Genomic DNA. Translation: CAA60935.1. Frameshift.
Z49513 Genomic DNA. Translation: CAA89537.1. Frameshift.
AY260895 Genomic DNA. Translation: AAP21763.1.
AY557910 Genomic DNA. Translation: AAS56236.1.
BK006943 Genomic DNA. Translation: DAA08805.1.
PIRiS55201.
RefSeqiNP_012547.2. NM_001181671.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33769. 11 interactions.
IntActiP47088. 54 interactions.
MINTiMINT-4491536.
STRINGi4932.YJR013W.

Protein family/group databases

CAZyiGT50. Glycosyltransferase Family 50.

Proteomic databases

MaxQBiP47088.
PaxDbiP47088.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJR013W; YJR013W; YJR013W.
GeneIDi853470.
KEGGisce:YJR013W.

Organism-specific databases

CYGDiYJR013w.
SGDiS000003774. GPI14.

Phylogenomic databases

eggNOGiNOG268708.
GeneTreeiENSGT00390000017728.
HOGENOMiHOG000186884.
InParanoidiP47088.
KOiK05284.
OMAiEFLEHTY.
OrthoDBiEOG77DJGT.

Enzyme and pathway databases

UniPathwayiUPA00196.
BioCyciYEAST:G3O-31658-MONOMER.

Miscellaneous databases

NextBioi974068.
PROiP47088.

Gene expression databases

GenevestigatoriP47088.

Family and domain databases

InterProiIPR007704. Mannosyltransferase_DXD.
[Graphical view]
PANTHERiPTHR12886. PTHR12886. 1 hit.
PfamiPF05007. Mannosyl_trans. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Reinvestigation of the Saccharomyces cerevisiae genome annotation by comparison to the genome of a related fungus: Ashbya gossypii."
    Brachat S., Dietrich F.S., Voegeli S., Zhang Z., Stuart L., Lerch A., Gates K., Gaffney T.D., Philippsen P.
    Genome Biol. 4:R45.1-R45.13(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 14-73, IDENTIFICATION OF FRAMESHIFT.
    Strain: ATCC 204511 / S288c / AB972.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 99-403.
    Strain: ATCC 204508 / S288c.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. "Characterization of GPI14/YJR013w mutation that induces the cell wall integrity signalling pathway and results in increased protein production in Saccharomyces cerevisiae."
    Davydenko S.G., Feng D., Jaentti J., Keraenen S.
    Yeast 22:993-1009(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.

Entry informationi

Entry nameiGPI14_YEAST
AccessioniPrimary (citable) accession number: P47088
Secondary accession number(s): D6VWI9, E9P8R4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 31, 1996
Last sequence update: July 24, 2006
Last modified: March 31, 2015
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.