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P47088

- GPI14_YEAST

UniProt

P47088 - GPI14_YEAST

Protein

GPI mannosyltransferase 1

Gene

GPI14

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 2 (25 Jul 2006)
      Previous versions | rss
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    Functioni

    Mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers the first alpha-1,4-mannose to GlcN-acyl-PI during GPI precursor assembly. Required for cell wall integrity.1 Publication

    Pathwayi

    GO - Molecular functioni

    1. mannosyltransferase activity Source: SGD

    GO - Biological processi

    1. fungal-type cell wall organization Source: SGD
    2. GPI anchor biosynthetic process Source: SGD
    3. mannosylation Source: GOC

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Cell wall biogenesis/degradation, GPI-anchor biosynthesis

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31658-MONOMER.
    UniPathwayiUPA00196.

    Protein family/group databases

    CAZyiGT50. Glycosyltransferase Family 50.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GPI mannosyltransferase 1 (EC:2.4.1.-)
    Alternative name(s):
    GPI mannosyltransferase I
    Short name:
    GPI-MT-I
    Glycosylphosphatidylinositol-anchor biosynthesis protein 14
    Gene namesi
    Name:GPI14
    Synonyms:PMH1
    Ordered Locus Names:YJR013W
    ORF Names:J1444, YJR83.11
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome X

    Organism-specific databases

    CYGDiYJR013w.
    SGDiS000003774. GPI14.

    Subcellular locationi

    Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: SGD
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 403403GPI mannosyltransferase 1PRO_0000203084Add
    BLAST

    Proteomic databases

    MaxQBiP47088.
    PaxDbiP47088.

    Expressioni

    Gene expression databases

    GenevestigatoriP47088.

    Interactioni

    Protein-protein interaction databases

    BioGridi33769. 11 interactions.
    IntActiP47088. 54 interactions.
    MINTiMINT-4491536.
    STRINGi4932.YJR013W.

    Structurei

    3D structure databases

    ProteinModelPortaliP47088.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 44CytoplasmicSequence Analysis
    Topological domaini26 – 7853LumenalSequence AnalysisAdd
    BLAST
    Topological domaini100 – 11011CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini132 – 1321LumenalSequence Analysis
    Topological domaini150 – 16011CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini182 – 19312LumenalSequence AnalysisAdd
    BLAST
    Topological domaini215 – 26652CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini288 – 31023LumenalSequence AnalysisAdd
    BLAST
    Topological domaini332 – 3343CytoplasmicSequence Analysis
    Topological domaini356 – 3616LumenalSequence Analysis
    Topological domaini383 – 40321CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei5 – 2521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei79 – 9921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei111 – 13121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei133 – 14917HelicalSequence AnalysisAdd
    BLAST
    Transmembranei161 – 18121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei194 – 21421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei267 – 28721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei311 – 33121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei335 – 35521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei362 – 38221HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PIGM family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG268708.
    GeneTreeiENSGT00390000017728.
    HOGENOMiHOG000186884.
    KOiK05284.
    OMAiEFLEHTY.
    OrthoDBiEOG77DJGT.

    Family and domain databases

    InterProiIPR007704. Mannosyltransferase_DXD.
    [Graphical view]
    PANTHERiPTHR12886. PTHR12886. 1 hit.
    PfamiPF05007. Mannosyl_trans. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P47088-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTGEEWGLTV LSFLVRVGFF LFGIYQDANF KVRYTDIDYF VFHDAAKYVY    50
    EGKSPYARDT YRYTPLLSWL LVPNHYFGWF HLGKVIFVIF DLVTGLIIMK 100
    LLNQAISRKR ALILESIWLL NPMVITISTR GNAESVLCCL IMFTLFFLQK 150
    SRYTLAGILY GLSIHFKIYP IIYCIPIAIF IYYNKRNQGP RTQLTSLLNI 200
    GLSTLTTLLG CGWAMYKIYG YEFLDQAYLY HLYRTDHRHN FSVWNMLLYL 250
    DSANKENGES NLSRYAFVPQ LLLVLVTGCL EWWNPTFDNL LRVLFVQTFA 300
    FVTYNKVCTS QYFVWYLIFL PFYLSRTHIG WKKGLLMATL WVGTQGIWLS 350
    QGYYLEFEGK NVFYPGLFIA SVLFFVTNVW LLGQFITDIK IPTQPTVSNK 400
    KNN 403
    Length:403
    Mass (Da):47,166
    Last modified:July 25, 2006 - v2
    Checksum:iD1017701557FDB0F
    GO

    Sequence cautioni

    The sequence CAA60935.1 differs from that shown. Reason: Frameshift at position 40.
    The sequence CAA89537.1 differs from that shown. Reason: Frameshift at position 40.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X87611 Genomic DNA. Translation: CAA60935.1. Frameshift.
    Z49513 Genomic DNA. Translation: CAA89537.1. Frameshift.
    AY260895 Genomic DNA. Translation: AAP21763.1.
    AY557910 Genomic DNA. Translation: AAS56236.1.
    BK006943 Genomic DNA. Translation: DAA08805.1.
    PIRiS55201.
    RefSeqiNP_012547.2. NM_001181671.1.

    Genome annotation databases

    EnsemblFungiiYJR013W; YJR013W; YJR013W.
    GeneIDi853470.
    KEGGisce:YJR013W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X87611 Genomic DNA. Translation: CAA60935.1 . Frameshift.
    Z49513 Genomic DNA. Translation: CAA89537.1 . Frameshift.
    AY260895 Genomic DNA. Translation: AAP21763.1 .
    AY557910 Genomic DNA. Translation: AAS56236.1 .
    BK006943 Genomic DNA. Translation: DAA08805.1 .
    PIRi S55201.
    RefSeqi NP_012547.2. NM_001181671.1.

    3D structure databases

    ProteinModelPortali P47088.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33769. 11 interactions.
    IntActi P47088. 54 interactions.
    MINTi MINT-4491536.
    STRINGi 4932.YJR013W.

    Protein family/group databases

    CAZyi GT50. Glycosyltransferase Family 50.

    Proteomic databases

    MaxQBi P47088.
    PaxDbi P47088.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YJR013W ; YJR013W ; YJR013W .
    GeneIDi 853470.
    KEGGi sce:YJR013W.

    Organism-specific databases

    CYGDi YJR013w.
    SGDi S000003774. GPI14.

    Phylogenomic databases

    eggNOGi NOG268708.
    GeneTreei ENSGT00390000017728.
    HOGENOMi HOG000186884.
    KOi K05284.
    OMAi EFLEHTY.
    OrthoDBi EOG77DJGT.

    Enzyme and pathway databases

    UniPathwayi UPA00196 .
    BioCyci YEAST:G3O-31658-MONOMER.

    Miscellaneous databases

    NextBioi 974068.
    PROi P47088.

    Gene expression databases

    Genevestigatori P47088.

    Family and domain databases

    InterProi IPR007704. Mannosyltransferase_DXD.
    [Graphical view ]
    PANTHERi PTHR12886. PTHR12886. 1 hit.
    Pfami PF05007. Mannosyl_trans. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Reinvestigation of the Saccharomyces cerevisiae genome annotation by comparison to the genome of a related fungus: Ashbya gossypii."
      Brachat S., Dietrich F.S., Voegeli S., Zhang Z., Stuart L., Lerch A., Gates K., Gaffney T.D., Philippsen P.
      Genome Biol. 4:R45.1-R45.13(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 14-73, IDENTIFICATION OF FRAMESHIFT.
      Strain: ATCC 204511 / S288c / AB972.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 99-403.
      Strain: ATCC 204508 / S288c.
    5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    6. "Characterization of GPI14/YJR013w mutation that induces the cell wall integrity signalling pathway and results in increased protein production in Saccharomyces cerevisiae."
      Davydenko S.G., Feng D., Jaentti J., Keraenen S.
      Yeast 22:993-1009(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
      Kim H., Melen K., Oesterberg M., von Heijne G.
      Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: ATCC 208353 / W303-1A.

    Entry informationi

    Entry nameiGPI14_YEAST
    AccessioniPrimary (citable) accession number: P47088
    Secondary accession number(s): D6VWI9, E9P8R4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: July 25, 2006
    Last modified: October 1, 2014
    This is version 105 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3