GPI mannosyltransferase 1 - Saccharomyces cerevisiae (Baker's yeast)

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Reviewed, UniProtKB/Swiss-Prot P47088 (GPI14_YEAST)

Last modified June 16, 2009. Version 62. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    GPI mannosyltransferase 1
    EC=2.4.1.-
Alternative name(s):
    GPI mannosyltransferase I
      Short name=GPI-MT-I
    Glycosylphosphatidylinositol-anchor biosynthesis protein 14

Gene names

Name:	GPI14
Synonyms:	PMH1
Ordered Locus Names:	YJR013W
ORF Names:	J1444, YJR83.11

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length	403 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers the first alpha-1,4-mannose to GlcN-acyl-PI during GPI precursor assembly. Required for cell wall integrity. Ref.4
Pathway	Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein. Ref.3
Sequence similarities	Belongs to the PIGM family.
Sequence caution	The sequence CAA60935.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAA89537.1 differs from that shown. Reason: Erroneous gene model prediction.

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Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation GPI-anchor biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GPI anchor biosynthetic process Ref.4 Inferred from mutant phenotype. Source: SGD fungal-type cell wall organization Ref.4 Inferred from mutant phenotype. Source: SGD
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring hexosyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 403

403

GPI mannosyltransferase 1

PRO_0000203084

Regions

Topological domain

1 – 4

Cytoplasmic Potential

Transmembrane

5 – 25

Potential

Topological domain

26 – 78

Lumenal Potential

Transmembrane

79 – 99

Potential

Topological domain

100 – 110

Cytoplasmic Potential

Transmembrane

111 – 131

Potential

Topological domain

132

Lumenal Potential

Transmembrane

133 – 149

Potential

Topological domain

150 – 160

Cytoplasmic Potential

Transmembrane

161 – 181

Potential

Topological domain

182 – 193

Lumenal Potential

Transmembrane

194 – 214

Potential

Topological domain

215 – 266

Cytoplasmic Potential

Transmembrane

267 – 287

Potential

Topological domain

288 – 310

Lumenal Potential

Transmembrane

311 – 331

Potential

Topological domain

332 – 334

Cytoplasmic Potential

Transmembrane

335 – 355

Potential

Topological domain

356 – 361

Lumenal Potential

Transmembrane

362 – 382

Potential

Topological domain

383 – 403

Cytoplasmic Potential

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Sequences

Sequence

Length

Mass (Da)

Tools

P47088-1 [UniParc].

Last modified July 25, 2006. Version 2.
Checksum: D1017701557FDB0F
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FASTA

403

47,166

        10         20         30         40         50         60 
MTGEEWGLTV LSFLVRVGFF LFGIYQDANF KVRYTDIDYF VFHDAAKYVY EGKSPYARDT 

        70         80         90        100        110        120 
YRYTPLLSWL LVPNHYFGWF HLGKVIFVIF DLVTGLIIMK LLNQAISRKR ALILESIWLL 

       130        140        150        160        170        180 
NPMVITISTR GNAESVLCCL IMFTLFFLQK SRYTLAGILY GLSIHFKIYP IIYCIPIAIF 

       190        200        210        220        230        240 
IYYNKRNQGP RTQLTSLLNI GLSTLTTLLG CGWAMYKIYG YEFLDQAYLY HLYRTDHRHN 

       250        260        270        280        290        300 
FSVWNMLLYL DSANKENGES NLSRYAFVPQ LLLVLVTGCL EWWNPTFDNL LRVLFVQTFA 

       310        320        330        340        350        360 
FVTYNKVCTS QYFVWYLIFL PFYLSRTHIG WKKGLLMATL WVGTQGIWLS QGYYLEFEGK 

       370        380        390        400 
NVFYPGLFIA SVLFFVTNVW LLGQFITDIK IPTQPTVSNK KNN

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 99-403. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Characterization of GPI14/YJR013w mutation that induces the cell wall integrity signalling pathway and results in increased protein production in Saccharomyces cerevisiae." Davydenko S.G., Feng D., Jaentti J., Keraenen S. Yeast 22:993-1009(2005) [PubMed: 16134120] [Abstract] Cited for: FUNCTION.
[5]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	X87611 Genomic DNA. Translation: CAA60935.1. Sequence problems. Z49513 Genomic DNA. Translation: CAA89537.1. Sequence problems. AY557910 Genomic DNA. Translation: AAS56236.1.
PIR	S55201.
RefSeq	NP_012547.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P47088. 53 interactions.
Protein family/group databases
CAZy	GT50. Glycosyltransferase Family 50.
Genome annotation databases
Ensembl	YJR013W. Saccharomyces cerevisiae. [Contig view]
GeneID	853470.
GenomeReviews	Gene locus YJR013W in contig Y13136_GR.
KEGG	sce:YJR013W.
NMPDR	fig\|4932.3.peg.3521.
Organism-specific databases
CYGD	YJR013w.
SGD	S000003774. GPI14.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P47088.
OMA	P47088. NFSPYFY.
Gene expression databases
ArrayExpress	P47088.
GermOnline	YJR013W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007704. Mannosyltransferase_DXD. [Graphical view]
PANTHER	PTHR12886. Mannos_trans_DXD. 1 hit.
Pfam	PF05007. Mannosyl_trans. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	974068.

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Entry information

Entry name

GPI14_YEAST

Accession

Primary (citable) accession number: P47088

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	July 25, 2006
Last modified:	June 16, 2009
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents