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P47088

- GPI14_YEAST

UniProt

P47088 - GPI14_YEAST

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Protein
GPI mannosyltransferase 1
Gene
GPI14, PMH1, YJR013W, J1444, YJR83.11
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers the first alpha-1,4-mannose to GlcN-acyl-PI during GPI precursor assembly. Required for cell wall integrity.1 Publication

Pathwayi

GO - Molecular functioni

  1. mannosyltransferase activity Source: SGD

GO - Biological processi

  1. GPI anchor biosynthetic process Source: SGD
  2. fungal-type cell wall organization Source: SGD
  3. mannosylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Cell wall biogenesis/degradation, GPI-anchor biosynthesis

Enzyme and pathway databases

BioCyciYEAST:G3O-31658-MONOMER.
UniPathwayiUPA00196.

Protein family/group databases

CAZyiGT50. Glycosyltransferase Family 50.

Names & Taxonomyi

Protein namesi
Recommended name:
GPI mannosyltransferase 1 (EC:2.4.1.-)
Alternative name(s):
GPI mannosyltransferase I
Short name:
GPI-MT-I
Glycosylphosphatidylinositol-anchor biosynthesis protein 14
Gene namesi
Name:GPI14
Synonyms:PMH1
Ordered Locus Names:YJR013W
ORF Names:J1444, YJR83.11
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

CYGDiYJR013w.
SGDiS000003774. GPI14.

Subcellular locationi

Endoplasmic reticulum membrane; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 44Cytoplasmic Reviewed prediction
Transmembranei5 – 2521Helical; Reviewed prediction
Add
BLAST
Topological domaini26 – 7853Lumenal Reviewed prediction
Add
BLAST
Transmembranei79 – 9921Helical; Reviewed prediction
Add
BLAST
Topological domaini100 – 11011Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei111 – 13121Helical; Reviewed prediction
Add
BLAST
Topological domaini132 – 1321Lumenal Reviewed prediction
Transmembranei133 – 14917Helical; Reviewed prediction
Add
BLAST
Topological domaini150 – 16011Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei161 – 18121Helical; Reviewed prediction
Add
BLAST
Topological domaini182 – 19312Lumenal Reviewed prediction
Add
BLAST
Transmembranei194 – 21421Helical; Reviewed prediction
Add
BLAST
Topological domaini215 – 26652Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei267 – 28721Helical; Reviewed prediction
Add
BLAST
Topological domaini288 – 31023Lumenal Reviewed prediction
Add
BLAST
Transmembranei311 – 33121Helical; Reviewed prediction
Add
BLAST
Topological domaini332 – 3343Cytoplasmic Reviewed prediction
Transmembranei335 – 35521Helical; Reviewed prediction
Add
BLAST
Topological domaini356 – 3616Lumenal Reviewed prediction
Transmembranei362 – 38221Helical; Reviewed prediction
Add
BLAST
Topological domaini383 – 40321Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: SGD
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 403403GPI mannosyltransferase 1
PRO_0000203084Add
BLAST

Proteomic databases

MaxQBiP47088.
PaxDbiP47088.

Expressioni

Gene expression databases

GenevestigatoriP47088.

Interactioni

Protein-protein interaction databases

BioGridi33769. 11 interactions.
IntActiP47088. 54 interactions.
MINTiMINT-4491536.
STRINGi4932.YJR013W.

Structurei

3D structure databases

ProteinModelPortaliP47088.

Family & Domainsi

Sequence similaritiesi

Belongs to the PIGM family.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG268708.
GeneTreeiENSGT00390000017728.
HOGENOMiHOG000186884.
KOiK05284.
OMAiEFLEHTY.
OrthoDBiEOG77DJGT.

Family and domain databases

InterProiIPR007704. Mannosyltransferase_DXD.
[Graphical view]
PANTHERiPTHR12886. PTHR12886. 1 hit.
PfamiPF05007. Mannosyl_trans. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47088-1 [UniParc]FASTAAdd to Basket

« Hide

MTGEEWGLTV LSFLVRVGFF LFGIYQDANF KVRYTDIDYF VFHDAAKYVY    50
EGKSPYARDT YRYTPLLSWL LVPNHYFGWF HLGKVIFVIF DLVTGLIIMK 100
LLNQAISRKR ALILESIWLL NPMVITISTR GNAESVLCCL IMFTLFFLQK 150
SRYTLAGILY GLSIHFKIYP IIYCIPIAIF IYYNKRNQGP RTQLTSLLNI 200
GLSTLTTLLG CGWAMYKIYG YEFLDQAYLY HLYRTDHRHN FSVWNMLLYL 250
DSANKENGES NLSRYAFVPQ LLLVLVTGCL EWWNPTFDNL LRVLFVQTFA 300
FVTYNKVCTS QYFVWYLIFL PFYLSRTHIG WKKGLLMATL WVGTQGIWLS 350
QGYYLEFEGK NVFYPGLFIA SVLFFVTNVW LLGQFITDIK IPTQPTVSNK 400
KNN 403
Length:403
Mass (Da):47,166
Last modified:July 25, 2006 - v2
Checksum:iD1017701557FDB0F
GO

Sequence cautioni

The sequence CAA60935.1 differs from that shown. Reason: Frameshift at position 40.
The sequence CAA89537.1 differs from that shown. Reason: Frameshift at position 40.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X87611 Genomic DNA. Translation: CAA60935.1. Frameshift.
Z49513 Genomic DNA. Translation: CAA89537.1. Frameshift.
AY260895 Genomic DNA. Translation: AAP21763.1.
AY557910 Genomic DNA. Translation: AAS56236.1.
BK006943 Genomic DNA. Translation: DAA08805.1.
PIRiS55201.
RefSeqiNP_012547.2. NM_001181671.1.

Genome annotation databases

EnsemblFungiiYJR013W; YJR013W; YJR013W.
GeneIDi853470.
KEGGisce:YJR013W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X87611 Genomic DNA. Translation: CAA60935.1 . Frameshift.
Z49513 Genomic DNA. Translation: CAA89537.1 . Frameshift.
AY260895 Genomic DNA. Translation: AAP21763.1 .
AY557910 Genomic DNA. Translation: AAS56236.1 .
BK006943 Genomic DNA. Translation: DAA08805.1 .
PIRi S55201.
RefSeqi NP_012547.2. NM_001181671.1.

3D structure databases

ProteinModelPortali P47088.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33769. 11 interactions.
IntActi P47088. 54 interactions.
MINTi MINT-4491536.
STRINGi 4932.YJR013W.

Protein family/group databases

CAZyi GT50. Glycosyltransferase Family 50.

Proteomic databases

MaxQBi P47088.
PaxDbi P47088.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YJR013W ; YJR013W ; YJR013W .
GeneIDi 853470.
KEGGi sce:YJR013W.

Organism-specific databases

CYGDi YJR013w.
SGDi S000003774. GPI14.

Phylogenomic databases

eggNOGi NOG268708.
GeneTreei ENSGT00390000017728.
HOGENOMi HOG000186884.
KOi K05284.
OMAi EFLEHTY.
OrthoDBi EOG77DJGT.

Enzyme and pathway databases

UniPathwayi UPA00196 .
BioCyci YEAST:G3O-31658-MONOMER.

Miscellaneous databases

NextBioi 974068.
PROi P47088.

Gene expression databases

Genevestigatori P47088.

Family and domain databases

InterProi IPR007704. Mannosyltransferase_DXD.
[Graphical view ]
PANTHERi PTHR12886. PTHR12886. 1 hit.
Pfami PF05007. Mannosyl_trans. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Reinvestigation of the Saccharomyces cerevisiae genome annotation by comparison to the genome of a related fungus: Ashbya gossypii."
    Brachat S., Dietrich F.S., Voegeli S., Zhang Z., Stuart L., Lerch A., Gates K., Gaffney T.D., Philippsen P.
    Genome Biol. 4:R45.1-R45.13(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 14-73, IDENTIFICATION OF FRAMESHIFT.
    Strain: ATCC 204511 / S288c / AB972.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 99-403.
    Strain: ATCC 204508 / S288c.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. "Characterization of GPI14/YJR013w mutation that induces the cell wall integrity signalling pathway and results in increased protein production in Saccharomyces cerevisiae."
    Davydenko S.G., Feng D., Jaentti J., Keraenen S.
    Yeast 22:993-1009(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.

Entry informationi

Entry nameiGPI14_YEAST
AccessioniPrimary (citable) accession number: P47088
Secondary accession number(s): D6VWI9, E9P8R4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: July 25, 2006
Last modified: June 11, 2014
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3

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