GPI mannosyltransferase 1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P47088 (GPI14_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 95. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
GPI mannosyltransferase 1
EC=2.4.1.-

Alternative name(s):
GPI mannosyltransferase I
Short name=GPI-MT-I
Glycosylphosphatidylinositol-anchor biosynthesis protein 14

Gene names

Name:	GPI14
Synonyms:	PMH1
Ordered Locus Names:	YJR013W
ORF Names:	J1444, YJR83.11

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	403 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers the first alpha-1,4-mannose to GlcN-acyl-PI during GPI precursor assembly. Required for cell wall integrity. Ref.6
Pathway	Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.5.
Sequence similarities	Belongs to the PIGM family.
Sequence caution	The sequence CAA60935.1 differs from that shown. Reason: Frameshift at position 40. The sequence CAA89537.1 differs from that shown. Reason: Frameshift at position 40.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation GPI-anchor biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	GPI anchor biosynthetic process Inferred from mutant phenotype Ref.6. Source: SGD fungal-type cell wall organization Inferred from mutant phenotype Ref.6. Source: SGD
Cellular_component	endoplasmic reticulum membrane Inferred from sequence or structural similarity PubMed 11226175. Source: SGD integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD
Molecular_function	mannosyltransferase activity Inferred from sequence or structural similarity PubMed 11226175. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 403

403

GPI mannosyltransferase 1

PRO_0000203084

Regions

Topological domain

1 – 4

Cytoplasmic Potential

Transmembrane

5 – 25

Helical; Potential

Topological domain

26 – 78

Lumenal Potential

Transmembrane

79 – 99

Helical; Potential

Topological domain

100 – 110

Cytoplasmic Potential

Transmembrane

111 – 131

Helical; Potential

Topological domain

132

Lumenal Potential

Transmembrane

133 – 149

Helical; Potential

Topological domain

150 – 160

Cytoplasmic Potential

Transmembrane

161 – 181

Helical; Potential

Topological domain

182 – 193

Lumenal Potential

Transmembrane

194 – 214

Helical; Potential

Topological domain

215 – 266

Cytoplasmic Potential

Transmembrane

267 – 287

Helical; Potential

Topological domain

288 – 310

Lumenal Potential

Transmembrane

311 – 331

Helical; Potential

Topological domain

332 – 334

Cytoplasmic Potential

Transmembrane

335 – 355

Helical; Potential

Topological domain

356 – 361

Lumenal Potential

Transmembrane

362 – 382

Helical; Potential

Topological domain

383 – 403

Cytoplasmic Potential

Sequences

Sequence

Length

Mass (Da)

Tools

P47088 [UniParc].

Last modified July 25, 2006. Version 2.
Checksum: D1017701557FDB0F
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FASTA

403

47,166

        10         20         30         40         50         60 
MTGEEWGLTV LSFLVRVGFF LFGIYQDANF KVRYTDIDYF VFHDAAKYVY EGKSPYARDT 

        70         80         90        100        110        120 
YRYTPLLSWL LVPNHYFGWF HLGKVIFVIF DLVTGLIIMK LLNQAISRKR ALILESIWLL 

       130        140        150        160        170        180 
NPMVITISTR GNAESVLCCL IMFTLFFLQK SRYTLAGILY GLSIHFKIYP IIYCIPIAIF 

       190        200        210        220        230        240 
IYYNKRNQGP RTQLTSLLNI GLSTLTTLLG CGWAMYKIYG YEFLDQAYLY HLYRTDHRHN 

       250        260        270        280        290        300 
FSVWNMLLYL DSANKENGES NLSRYAFVPQ LLLVLVTGCL EWWNPTFDNL LRVLFVQTFA 

       310        320        330        340        350        360 
FVTYNKVCTS QYFVWYLIFL PFYLSRTHIG WKKGLLMATL WVGTQGIWLS QGYYLEFEGK 

       370        380        390        400 
NVFYPGLFIA SVLFFVTNVW LLGQFITDIK IPTQPTVSNK KNN

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Reinvestigation of the Saccharomyces cerevisiae genome annotation by comparison to the genome of a related fungus: Ashbya gossypii." Brachat S., Dietrich F.S., Voegeli S., Zhang Z., Stuart L., Lerch A., Gates K., Gaffney T.D., Philippsen P. Genome Biol. 4:R45.1-R45.13(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 14-73, IDENTIFICATION OF FRAMESHIFT. Strain: ATCC 204511 / S288c / AB972.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 99-403. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Characterization of GPI14/YJR013w mutation that induces the cell wall integrity signalling pathway and results in increased protein production in Saccharomyces cerevisiae." Davydenko S.G., Feng D., Jaentti J., Keraenen S. Yeast 22:993-1009(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X87611 Genomic DNA. Translation: CAA60935.1. Frameshift. Z49513 Genomic DNA. Translation: CAA89537.1. Frameshift. AY260895 Genomic DNA. Translation: AAP21763.1. AY557910 Genomic DNA. Translation: AAS56236.1. BK006943 Genomic DNA. Translation: DAA08805.1.
PIR	S55201.
RefSeq	NP_012547.2. NM_001181671.1.
3D structure databases
ProteinModelPortal	P47088.
ModBase	Search...
Protein-protein interaction databases
IntAct	P47088. 54 interactions.
STRING	4932.YJR013W.
Protein family/group databases
CAZy	GT50. Glycosyltransferase Family 50.
Proteomic databases
PaxDb	P47088.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJR013W; YJR013W; YJR013W.
GeneID	853470.
KEGG	sce:YJR013W.
Organism-specific databases
CYGD	YJR013w.
SGD	S000003774. GPI14.
Phylogenomic databases
eggNOG	NOG268708.
GeneTree	ENSGT00390000017728.
HOGENOM	HOG000186884.
KO	K05284.
OMA	EFLEHTY.
OrthoDB	EOG4643N0.
Enzyme and pathway databases
UniPathway	UPA00196.
Gene expression databases
Genevestigator	P47088.
GermOnline	YJR013W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007704. Mannosyltransferase_DXD. [Graphical view]
PANTHER	PTHR12886. PTHR12886. 1 hit.
Pfam	PF05007. Mannosyl_trans. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	974068.

Entry information

Entry name

GPI14_YEAST

Accession

Primary (citable) accession number: P47088
Secondary accession number(s): D6VWI9, E9P8R4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	July 25, 2006
Last modified:	April 3, 2013
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents