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P47088 (GPI14_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
GPI mannosyltransferase 1

EC=2.4.1.-
Alternative name(s):
GPI mannosyltransferase I
Short name=GPI-MT-I
Glycosylphosphatidylinositol-anchor biosynthesis protein 14
Gene names
Name:GPI14
Synonyms:PMH1
Ordered Locus Names:YJR013W
ORF Names:J1444, YJR83.11
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length403 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers the first alpha-1,4-mannose to GlcN-acyl-PI during GPI precursor assembly. Required for cell wall integrity. Ref.6

Pathway

Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.5.

Sequence similarities

Belongs to the PIGM family.

Sequence caution

The sequence CAA60935.1 differs from that shown. Reason: Frameshift at position 40.

The sequence CAA89537.1 differs from that shown. Reason: Frameshift at position 40.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 403403GPI mannosyltransferase 1
PRO_0000203084

Regions

Topological domain1 – 44Cytoplasmic Potential
Transmembrane5 – 2521Helical; Potential
Topological domain26 – 7853Lumenal Potential
Transmembrane79 – 9921Helical; Potential
Topological domain100 – 11011Cytoplasmic Potential
Transmembrane111 – 13121Helical; Potential
Topological domain1321Lumenal Potential
Transmembrane133 – 14917Helical; Potential
Topological domain150 – 16011Cytoplasmic Potential
Transmembrane161 – 18121Helical; Potential
Topological domain182 – 19312Lumenal Potential
Transmembrane194 – 21421Helical; Potential
Topological domain215 – 26652Cytoplasmic Potential
Transmembrane267 – 28721Helical; Potential
Topological domain288 – 31023Lumenal Potential
Transmembrane311 – 33121Helical; Potential
Topological domain332 – 3343Cytoplasmic Potential
Transmembrane335 – 35521Helical; Potential
Topological domain356 – 3616Lumenal Potential
Transmembrane362 – 38221Helical; Potential
Topological domain383 – 40321Cytoplasmic Potential

Sequences

Sequence LengthMass (Da)Tools
P47088 [UniParc].

Last modified July 25, 2006. Version 2.
Checksum: D1017701557FDB0F

FASTA40347,166
        10         20         30         40         50         60 
MTGEEWGLTV LSFLVRVGFF LFGIYQDANF KVRYTDIDYF VFHDAAKYVY EGKSPYARDT 

        70         80         90        100        110        120 
YRYTPLLSWL LVPNHYFGWF HLGKVIFVIF DLVTGLIIMK LLNQAISRKR ALILESIWLL 

       130        140        150        160        170        180 
NPMVITISTR GNAESVLCCL IMFTLFFLQK SRYTLAGILY GLSIHFKIYP IIYCIPIAIF 

       190        200        210        220        230        240 
IYYNKRNQGP RTQLTSLLNI GLSTLTTLLG CGWAMYKIYG YEFLDQAYLY HLYRTDHRHN 

       250        260        270        280        290        300 
FSVWNMLLYL DSANKENGES NLSRYAFVPQ LLLVLVTGCL EWWNPTFDNL LRVLFVQTFA 

       310        320        330        340        350        360 
FVTYNKVCTS QYFVWYLIFL PFYLSRTHIG WKKGLLMATL WVGTQGIWLS QGYYLEFEGK 

       370        380        390        400 
NVFYPGLFIA SVLFFVTNVW LLGQFITDIK IPTQPTVSNK KNN 

« Hide

References

« Hide 'large scale' references
[1]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Reinvestigation of the Saccharomyces cerevisiae genome annotation by comparison to the genome of a related fungus: Ashbya gossypii."
Brachat S., Dietrich F.S., Voegeli S., Zhang Z., Stuart L., Lerch A., Gates K., Gaffney T.D., Philippsen P.
Genome Biol. 4:R45.1-R45.13(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 14-73, IDENTIFICATION OF FRAMESHIFT.
Strain: ATCC 204511 / S288c / AB972.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 99-403.
Strain: ATCC 204508 / S288c.
[5]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]"Characterization of GPI14/YJR013w mutation that induces the cell wall integrity signalling pathway and results in increased protein production in Saccharomyces cerevisiae."
Davydenko S.G., Feng D., Jaentti J., Keraenen S.
Yeast 22:993-1009(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X87611 Genomic DNA. Translation: CAA60935.1. Frameshift.
Z49513 Genomic DNA. Translation: CAA89537.1. Frameshift.
AY260895 Genomic DNA. Translation: AAP21763.1.
AY557910 Genomic DNA. Translation: AAS56236.1.
BK006943 Genomic DNA. Translation: DAA08805.1.
PIRS55201.
RefSeqNP_012547.2. NM_001181671.1.

3D structure databases

ProteinModelPortalP47088.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33769. 10 interactions.
IntActP47088. 54 interactions.
MINTMINT-4491536.
STRING4932.YJR013W.

Protein family/group databases

CAZyGT50. Glycosyltransferase Family 50.

Proteomic databases

PaxDbP47088.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJR013W; YJR013W; YJR013W.
GeneID853470.
KEGGsce:YJR013W.

Organism-specific databases

CYGDYJR013w.
SGDS000003774. GPI14.

Phylogenomic databases

eggNOGNOG268708.
GeneTreeENSGT00390000017728.
HOGENOMHOG000186884.
KOK05284.
OMAGWEFLEH.
OrthoDBEOG77DJGT.

Enzyme and pathway databases

BioCycYEAST:G3O-31658-MONOMER.
UniPathwayUPA00196.

Gene expression databases

GenevestigatorP47088.

Family and domain databases

InterProIPR007704. Mannosyltransferase_DXD.
[Graphical view]
PANTHERPTHR12886. PTHR12886. 1 hit.
PfamPF05007. Mannosyl_trans. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio974068.
PROP47088.

Entry information

Entry nameGPI14_YEAST
AccessionPrimary (citable) accession number: P47088
Secondary accession number(s): D6VWI9, E9P8R4
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: July 25, 2006
Last modified: April 16, 2014
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways