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P47082

- AVT1_YEAST

UniProt

P47082 - AVT1_YEAST

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Protein

Vacuolar amino acid transporter 1

Gene

AVT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Required for the vacuolar uptake of large neutral amino acids including tyrosine, glutamine, asparagine, isoleucine and leucine. Requires ATP for function.1 Publication

GO - Molecular functioni

  1. L-glutamine transmembrane transporter activity Source: SGD
  2. L-isoleucine transmembrane transporter activity Source: SGD
  3. L-tyrosine transmembrane transporter activity Source: SGD
  4. transporter activity Source: SGD

GO - Biological processi

  1. amino acid transport Source: SGD
  2. branched-chain amino acid transport Source: GOC
  3. cation transmembrane transport Source: GOC
  4. cation transport Source: GOC
  5. glutamine transport Source: GOC
  6. L-alpha-amino acid transmembrane transport Source: GOC
  7. L-amino acid transport Source: GOC
  8. neutral amino acid transport Source: GOC
  9. tyrosine transport Source: GOC
  10. vacuolar transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31647-MONOMER.

Protein family/group databases

TCDBi2.A.18.5.2. the amino acid/auxin permease (aaap) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar amino acid transporter 1
Gene namesi
Name:AVT1
Ordered Locus Names:YJR001W
ORF Names:J1409, YJR83.4
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

CYGDiYJR001w.
SGDiS000003761. AVT1.

Subcellular locationi

Vacuole membrane 1 Publication; Multi-pass membrane protein 1 Publication

GO - Cellular componenti

  1. fungal-type vacuole Source: SGD
  2. fungal-type vacuole membrane Source: SGD
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 602602Vacuolar amino acid transporter 1PRO_0000093834Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei8 – 81Phosphoserine1 Publication
Modified residuei15 – 151Phosphoserine1 Publication
Modified residuei35 – 351Phosphoserine1 Publication
Modified residuei181 – 1811Phosphothreonine2 Publications
Modified residuei187 – 1871Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP47082.
PaxDbiP47082.
PRIDEiP47082.

Expressioni

Gene expression databases

GenevestigatoriP47082.

Interactioni

Protein-protein interaction databases

BioGridi33757. 26 interactions.
DIPiDIP-5709N.
IntActiP47082. 2 interactions.
MINTiMINT-504618.
STRINGi4932.YJR001W.

Structurei

3D structure databases

ProteinModelPortaliP47082.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 208208CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini232 – 2409VacuolarSequence Analysis
Topological domaini262 – 28625CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini308 – 32114VacuolarSequence AnalysisAdd
BLAST
Topological domaini343 – 3442CytoplasmicSequence Analysis
Topological domaini366 – 38924VacuolarSequence AnalysisAdd
BLAST
Topological domaini411 – 42919CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini451 – 46616VacuolarSequence AnalysisAdd
BLAST
Topological domaini488 – 51730CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini539 – 5435VacuolarSequence Analysis
Topological domaini565 – 58016CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini602 – 6021VacuolarSequence Analysis

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei209 – 23123HelicalSequence AnalysisAdd
BLAST
Transmembranei241 – 26121HelicalSequence AnalysisAdd
BLAST
Transmembranei287 – 30721HelicalSequence AnalysisAdd
BLAST
Transmembranei322 – 34221HelicalSequence AnalysisAdd
BLAST
Transmembranei345 – 36521HelicalSequence AnalysisAdd
BLAST
Transmembranei390 – 41021HelicalSequence AnalysisAdd
BLAST
Transmembranei430 – 45021HelicalSequence AnalysisAdd
BLAST
Transmembranei467 – 48721HelicalSequence AnalysisAdd
BLAST
Transmembranei518 – 53821HelicalSequence AnalysisAdd
BLAST
Transmembranei544 – 56421HelicalSequence AnalysisAdd
BLAST
Transmembranei581 – 60121HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0814.
GeneTreeiENSGT00490000043380.
HOGENOMiHOG000235065.
InParanoidiP47082.
KOiK15015.
OMAiKTDMRHP.
OrthoDBiEOG7MD4ZV.

Family and domain databases

InterProiIPR013057. AA_transpt_TM.
[Graphical view]
PfamiPF01490. Aa_trans. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47082-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPEQEPLSPN GRKRSEVHYI SIPLNRGSAF SPDDSVSQFQ SDGFMTRRQS
60 70 80 90 100
ILDHPVGSFK GVNSLSRFAT SLRRANSFRN IELNADNERS FFKESNDETY
110 120 130 140 150
DPDTLAPALD GRRLSVTLNN AGRPRITNLA NNDRVSTASM AIHDDDYGSI
160 170 180 190 200
QNSTIGDSGS ILRPTASLTE MMSGGAGRRF TNNDMDSIVV KRVEGVDGKV
210 220 230 240 250
VTLLAGQSTA PQTIFNSINV LIGIGLLALP LGLKYAGWVI GLTMLAIFAL
260 270 280 290 300
ATFCTAELLS RCLDTDPTLI SYADLGYAAF GTKGRALISA LFTLDLLGSG
310 320 330 340 350
VSLVILFGDS LNALFPQYST TFFKIVSFFI VTPPVFIPLS VLSNISLLGI
360 370 380 390 400
LSTTGTVLVI CCCGLYKSSS PGSLVNPMET SMWPIDLKHL CLSIGLLSAC
410 420 430 440 450
WGGHAVFPNL KTDMRHPDKF KDCLKTTYKI TSVTDIGTAV IGFLMFGNLV
460 470 480 490 500
KDEITKNVLL TEGYPKFVYG LISALMTIIP IAKTPLNARP IVSVLDVLMN
510 520 530 540 550
VQHIDEAASA IKRRAAKGLQ VFNRIFINVV FVLIAINFPE FDKIIAFLGA
560 570 580 590 600
GLCFTICLIL PCWFYLRLCK TTIKPWERVA CHVTICISVV LSTLGVGAAI

IS
Length:602
Mass (Da):65,346
Last modified:February 1, 1996 - v1
Checksum:iC2F321E76C21C2B4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X87611 Genomic DNA. Translation: CAA60922.1.
Z49501 Genomic DNA. Translation: CAA89523.1.
BK006943 Genomic DNA. Translation: DAA08791.1.
PIRiS55188.
RefSeqiNP_012534.1. NM_001181658.1.

Genome annotation databases

EnsemblFungiiYJR001W; YJR001W; YJR001W.
GeneIDi853457.
KEGGisce:YJR001W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X87611 Genomic DNA. Translation: CAA60922.1 .
Z49501 Genomic DNA. Translation: CAA89523.1 .
BK006943 Genomic DNA. Translation: DAA08791.1 .
PIRi S55188.
RefSeqi NP_012534.1. NM_001181658.1.

3D structure databases

ProteinModelPortali P47082.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33757. 26 interactions.
DIPi DIP-5709N.
IntActi P47082. 2 interactions.
MINTi MINT-504618.
STRINGi 4932.YJR001W.

Protein family/group databases

TCDBi 2.A.18.5.2. the amino acid/auxin permease (aaap) family.

Proteomic databases

MaxQBi P47082.
PaxDbi P47082.
PRIDEi P47082.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YJR001W ; YJR001W ; YJR001W .
GeneIDi 853457.
KEGGi sce:YJR001W.

Organism-specific databases

CYGDi YJR001w.
SGDi S000003761. AVT1.

Phylogenomic databases

eggNOGi COG0814.
GeneTreei ENSGT00490000043380.
HOGENOMi HOG000235065.
InParanoidi P47082.
KOi K15015.
OMAi KTDMRHP.
OrthoDBi EOG7MD4ZV.

Enzyme and pathway databases

BioCyci YEAST:G3O-31647-MONOMER.

Miscellaneous databases

NextBioi 974029.

Gene expression databases

Genevestigatori P47082.

Family and domain databases

InterProi IPR013057. AA_transpt_TM.
[Graphical view ]
Pfami PF01490. Aa_trans. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "A family of yeast proteins mediating bidirectional vacuolar amino acid transport."
    Russnak R., Konczal D., McIntire S.L.
    J. Biol. Chem. 276:23849-23857(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-181 AND SER-187, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-8 AND SER-35, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-15; THR-181 AND SER-187, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiAVT1_YEAST
AccessioniPrimary (citable) accession number: P47082
Secondary accession number(s): D6VWH5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: October 29, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1820 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3