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Protein

Vacuolar amino acid transporter 1

Gene

AVT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for the vacuolar uptake of large neutral amino acids including tyrosine, glutamine, asparagine, isoleucine and leucine. Requires ATP for function.1 Publication

GO - Molecular functioni

  • L-glutamine transmembrane transporter activity Source: SGD
  • L-isoleucine transmembrane transporter activity Source: SGD
  • L-tyrosine transmembrane transporter activity Source: SGD

GO - Biological processi

  • amino acid transmembrane transport Source: SGD
  • amino acid transport Source: SGD
  • branched-chain amino acid transport Source: GOC
  • cation transmembrane transport Source: GOC
  • cation transport Source: GOC
  • glutamine transport Source: GOC
  • L-alpha-amino acid transmembrane transport Source: GOC
  • L-amino acid transport Source: GOC
  • neutral amino acid transport Source: GOC
  • tyrosine transport Source: GOC
  • vacuolar transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31647-MONOMER.
ReactomeiREACT_316139. Transport of inorganic cations/anions and amino acids/oligopeptides.
REACT_323419. GABA synthesis, release, reuptake and degradation.

Protein family/group databases

TCDBi2.A.18.5.2. the amino acid/auxin permease (aaap) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar amino acid transporter 1
Gene namesi
Name:AVT1
Ordered Locus Names:YJR001W
ORF Names:J1409, YJR83.4
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome X

Organism-specific databases

CYGDiYJR001w.
EuPathDBiFungiDB:YJR001W.
SGDiS000003761. AVT1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 208208CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei209 – 23123HelicalSequence AnalysisAdd
BLAST
Topological domaini232 – 2409VacuolarSequence Analysis
Transmembranei241 – 26121HelicalSequence AnalysisAdd
BLAST
Topological domaini262 – 28625CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei287 – 30721HelicalSequence AnalysisAdd
BLAST
Topological domaini308 – 32114VacuolarSequence AnalysisAdd
BLAST
Transmembranei322 – 34221HelicalSequence AnalysisAdd
BLAST
Topological domaini343 – 3442CytoplasmicSequence Analysis
Transmembranei345 – 36521HelicalSequence AnalysisAdd
BLAST
Topological domaini366 – 38924VacuolarSequence AnalysisAdd
BLAST
Transmembranei390 – 41021HelicalSequence AnalysisAdd
BLAST
Topological domaini411 – 42919CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei430 – 45021HelicalSequence AnalysisAdd
BLAST
Topological domaini451 – 46616VacuolarSequence AnalysisAdd
BLAST
Transmembranei467 – 48721HelicalSequence AnalysisAdd
BLAST
Topological domaini488 – 51730CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei518 – 53821HelicalSequence AnalysisAdd
BLAST
Topological domaini539 – 5435VacuolarSequence Analysis
Transmembranei544 – 56421HelicalSequence AnalysisAdd
BLAST
Topological domaini565 – 58016CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei581 – 60121HelicalSequence AnalysisAdd
BLAST
Topological domaini602 – 6021VacuolarSequence Analysis

GO - Cellular componenti

  • fungal-type vacuole Source: SGD
  • fungal-type vacuole membrane Source: SGD
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 602602Vacuolar amino acid transporter 1PRO_0000093834Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei8 – 81Phosphoserine1 Publication
Modified residuei15 – 151Phosphoserine1 Publication
Modified residuei35 – 351Phosphoserine1 Publication
Modified residuei181 – 1811Phosphothreonine2 Publications
Modified residuei187 – 1871Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP47082.
PaxDbiP47082.
PRIDEiP47082.

Interactioni

Protein-protein interaction databases

BioGridi33757. 25 interactions.
DIPiDIP-5709N.
IntActiP47082. 2 interactions.
MINTiMINT-504618.

Structurei

3D structure databases

ProteinModelPortaliP47082.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0814.
GeneTreeiENSGT00490000043380.
HOGENOMiHOG000235065.
InParanoidiP47082.
KOiK15015.
OMAiNALFPQY.
OrthoDBiEOG7MD4ZV.

Family and domain databases

InterProiIPR013057. AA_transpt_TM.
[Graphical view]
PfamiPF01490. Aa_trans. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47082-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPEQEPLSPN GRKRSEVHYI SIPLNRGSAF SPDDSVSQFQ SDGFMTRRQS
60 70 80 90 100
ILDHPVGSFK GVNSLSRFAT SLRRANSFRN IELNADNERS FFKESNDETY
110 120 130 140 150
DPDTLAPALD GRRLSVTLNN AGRPRITNLA NNDRVSTASM AIHDDDYGSI
160 170 180 190 200
QNSTIGDSGS ILRPTASLTE MMSGGAGRRF TNNDMDSIVV KRVEGVDGKV
210 220 230 240 250
VTLLAGQSTA PQTIFNSINV LIGIGLLALP LGLKYAGWVI GLTMLAIFAL
260 270 280 290 300
ATFCTAELLS RCLDTDPTLI SYADLGYAAF GTKGRALISA LFTLDLLGSG
310 320 330 340 350
VSLVILFGDS LNALFPQYST TFFKIVSFFI VTPPVFIPLS VLSNISLLGI
360 370 380 390 400
LSTTGTVLVI CCCGLYKSSS PGSLVNPMET SMWPIDLKHL CLSIGLLSAC
410 420 430 440 450
WGGHAVFPNL KTDMRHPDKF KDCLKTTYKI TSVTDIGTAV IGFLMFGNLV
460 470 480 490 500
KDEITKNVLL TEGYPKFVYG LISALMTIIP IAKTPLNARP IVSVLDVLMN
510 520 530 540 550
VQHIDEAASA IKRRAAKGLQ VFNRIFINVV FVLIAINFPE FDKIIAFLGA
560 570 580 590 600
GLCFTICLIL PCWFYLRLCK TTIKPWERVA CHVTICISVV LSTLGVGAAI

IS
Length:602
Mass (Da):65,346
Last modified:February 1, 1996 - v1
Checksum:iC2F321E76C21C2B4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87611 Genomic DNA. Translation: CAA60922.1.
Z49501 Genomic DNA. Translation: CAA89523.1.
BK006943 Genomic DNA. Translation: DAA08791.1.
PIRiS55188.
RefSeqiNP_012534.1. NM_001181658.1.

Genome annotation databases

EnsemblFungiiYJR001W; YJR001W; YJR001W.
GeneIDi853457.
KEGGisce:YJR001W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87611 Genomic DNA. Translation: CAA60922.1.
Z49501 Genomic DNA. Translation: CAA89523.1.
BK006943 Genomic DNA. Translation: DAA08791.1.
PIRiS55188.
RefSeqiNP_012534.1. NM_001181658.1.

3D structure databases

ProteinModelPortaliP47082.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33757. 25 interactions.
DIPiDIP-5709N.
IntActiP47082. 2 interactions.
MINTiMINT-504618.

Protein family/group databases

TCDBi2.A.18.5.2. the amino acid/auxin permease (aaap) family.

Proteomic databases

MaxQBiP47082.
PaxDbiP47082.
PRIDEiP47082.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJR001W; YJR001W; YJR001W.
GeneIDi853457.
KEGGisce:YJR001W.

Organism-specific databases

CYGDiYJR001w.
EuPathDBiFungiDB:YJR001W.
SGDiS000003761. AVT1.

Phylogenomic databases

eggNOGiCOG0814.
GeneTreeiENSGT00490000043380.
HOGENOMiHOG000235065.
InParanoidiP47082.
KOiK15015.
OMAiNALFPQY.
OrthoDBiEOG7MD4ZV.

Enzyme and pathway databases

BioCyciYEAST:G3O-31647-MONOMER.
ReactomeiREACT_316139. Transport of inorganic cations/anions and amino acids/oligopeptides.
REACT_323419. GABA synthesis, release, reuptake and degradation.

Miscellaneous databases

NextBioi974029.
PROiP47082.

Family and domain databases

InterProiIPR013057. AA_transpt_TM.
[Graphical view]
PfamiPF01490. Aa_trans. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "A family of yeast proteins mediating bidirectional vacuolar amino acid transport."
    Russnak R., Konczal D., McIntire S.L.
    J. Biol. Chem. 276:23849-23857(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-181 AND SER-187, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-8 AND SER-35, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-15; THR-181 AND SER-187, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiAVT1_YEAST
AccessioniPrimary (citable) accession number: P47082
Secondary accession number(s): D6VWH5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: July 22, 2015
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1820 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.