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P47082

- AVT1_YEAST

UniProt

P47082 - AVT1_YEAST

Protein

Vacuolar amino acid transporter 1

Gene

AVT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Required for the vacuolar uptake of large neutral amino acids including tyrosine, glutamine, asparagine, isoleucine and leucine. Requires ATP for function.1 Publication

    GO - Molecular functioni

    1. L-glutamine transmembrane transporter activity Source: SGD
    2. L-isoleucine transmembrane transporter activity Source: SGD
    3. L-tyrosine transmembrane transporter activity Source: SGD
    4. transporter activity Source: SGD

    GO - Biological processi

    1. amino acid transport Source: SGD
    2. branched-chain amino acid transport Source: GOC
    3. cation transport Source: GOC
    4. glutamine transport Source: GOC
    5. L-alpha-amino acid transmembrane transport Source: GOC
    6. L-amino acid transport Source: GOC
    7. neutral amino acid transport Source: GOC
    8. tyrosine transport Source: GOC
    9. vacuolar transport Source: SGD

    Keywords - Biological processi

    Amino-acid transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31647-MONOMER.

    Protein family/group databases

    TCDBi2.A.18.5.2. the amino acid/auxin permease (aaap) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Vacuolar amino acid transporter 1
    Gene namesi
    Name:AVT1
    Ordered Locus Names:YJR001W
    ORF Names:J1409, YJR83.4
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome X

    Organism-specific databases

    CYGDiYJR001w.
    SGDiS000003761. AVT1.

    Subcellular locationi

    Vacuole membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. fungal-type vacuole Source: SGD
    2. fungal-type vacuole membrane Source: SGD
    3. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane, Vacuole

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 602602Vacuolar amino acid transporter 1PRO_0000093834Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei8 – 81Phosphoserine1 Publication
    Modified residuei15 – 151Phosphoserine1 Publication
    Modified residuei35 – 351Phosphoserine1 Publication
    Modified residuei181 – 1811Phosphothreonine2 Publications
    Modified residuei187 – 1871Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP47082.
    PaxDbiP47082.
    PRIDEiP47082.

    Expressioni

    Gene expression databases

    GenevestigatoriP47082.

    Interactioni

    Protein-protein interaction databases

    BioGridi33757. 26 interactions.
    DIPiDIP-5709N.
    IntActiP47082. 2 interactions.
    MINTiMINT-504618.
    STRINGi4932.YJR001W.

    Structurei

    3D structure databases

    ProteinModelPortaliP47082.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 208208CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini232 – 2409VacuolarSequence Analysis
    Topological domaini262 – 28625CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini308 – 32114VacuolarSequence AnalysisAdd
    BLAST
    Topological domaini343 – 3442CytoplasmicSequence Analysis
    Topological domaini366 – 38924VacuolarSequence AnalysisAdd
    BLAST
    Topological domaini411 – 42919CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini451 – 46616VacuolarSequence AnalysisAdd
    BLAST
    Topological domaini488 – 51730CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini539 – 5435VacuolarSequence Analysis
    Topological domaini565 – 58016CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini602 – 6021VacuolarSequence Analysis

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei209 – 23123HelicalSequence AnalysisAdd
    BLAST
    Transmembranei241 – 26121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei287 – 30721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei322 – 34221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei345 – 36521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei390 – 41021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei430 – 45021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei467 – 48721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei518 – 53821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei544 – 56421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei581 – 60121HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0814.
    GeneTreeiENSGT00490000043380.
    HOGENOMiHOG000235065.
    KOiK15015.
    OMAiKTDMRHP.
    OrthoDBiEOG7MD4ZV.

    Family and domain databases

    InterProiIPR013057. AA_transpt_TM.
    [Graphical view]
    PfamiPF01490. Aa_trans. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P47082-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPEQEPLSPN GRKRSEVHYI SIPLNRGSAF SPDDSVSQFQ SDGFMTRRQS    50
    ILDHPVGSFK GVNSLSRFAT SLRRANSFRN IELNADNERS FFKESNDETY 100
    DPDTLAPALD GRRLSVTLNN AGRPRITNLA NNDRVSTASM AIHDDDYGSI 150
    QNSTIGDSGS ILRPTASLTE MMSGGAGRRF TNNDMDSIVV KRVEGVDGKV 200
    VTLLAGQSTA PQTIFNSINV LIGIGLLALP LGLKYAGWVI GLTMLAIFAL 250
    ATFCTAELLS RCLDTDPTLI SYADLGYAAF GTKGRALISA LFTLDLLGSG 300
    VSLVILFGDS LNALFPQYST TFFKIVSFFI VTPPVFIPLS VLSNISLLGI 350
    LSTTGTVLVI CCCGLYKSSS PGSLVNPMET SMWPIDLKHL CLSIGLLSAC 400
    WGGHAVFPNL KTDMRHPDKF KDCLKTTYKI TSVTDIGTAV IGFLMFGNLV 450
    KDEITKNVLL TEGYPKFVYG LISALMTIIP IAKTPLNARP IVSVLDVLMN 500
    VQHIDEAASA IKRRAAKGLQ VFNRIFINVV FVLIAINFPE FDKIIAFLGA 550
    GLCFTICLIL PCWFYLRLCK TTIKPWERVA CHVTICISVV LSTLGVGAAI 600
    IS 602
    Length:602
    Mass (Da):65,346
    Last modified:February 1, 1996 - v1
    Checksum:iC2F321E76C21C2B4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X87611 Genomic DNA. Translation: CAA60922.1.
    Z49501 Genomic DNA. Translation: CAA89523.1.
    BK006943 Genomic DNA. Translation: DAA08791.1.
    PIRiS55188.
    RefSeqiNP_012534.1. NM_001181658.1.

    Genome annotation databases

    EnsemblFungiiYJR001W; YJR001W; YJR001W.
    GeneIDi853457.
    KEGGisce:YJR001W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X87611 Genomic DNA. Translation: CAA60922.1 .
    Z49501 Genomic DNA. Translation: CAA89523.1 .
    BK006943 Genomic DNA. Translation: DAA08791.1 .
    PIRi S55188.
    RefSeqi NP_012534.1. NM_001181658.1.

    3D structure databases

    ProteinModelPortali P47082.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33757. 26 interactions.
    DIPi DIP-5709N.
    IntActi P47082. 2 interactions.
    MINTi MINT-504618.
    STRINGi 4932.YJR001W.

    Protein family/group databases

    TCDBi 2.A.18.5.2. the amino acid/auxin permease (aaap) family.

    Proteomic databases

    MaxQBi P47082.
    PaxDbi P47082.
    PRIDEi P47082.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YJR001W ; YJR001W ; YJR001W .
    GeneIDi 853457.
    KEGGi sce:YJR001W.

    Organism-specific databases

    CYGDi YJR001w.
    SGDi S000003761. AVT1.

    Phylogenomic databases

    eggNOGi COG0814.
    GeneTreei ENSGT00490000043380.
    HOGENOMi HOG000235065.
    KOi K15015.
    OMAi KTDMRHP.
    OrthoDBi EOG7MD4ZV.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31647-MONOMER.

    Miscellaneous databases

    NextBioi 974029.

    Gene expression databases

    Genevestigatori P47082.

    Family and domain databases

    InterProi IPR013057. AA_transpt_TM.
    [Graphical view ]
    Pfami PF01490. Aa_trans. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "A family of yeast proteins mediating bidirectional vacuolar amino acid transport."
      Russnak R., Konczal D., McIntire S.L.
      J. Biol. Chem. 276:23849-23857(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
      Kim H., Melen K., Oesterberg M., von Heijne G.
      Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: ATCC 208353 / W303-1A.
    6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-181 AND SER-187, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-8 AND SER-35, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-15; THR-181 AND SER-187, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiAVT1_YEAST
    AccessioniPrimary (citable) accession number: P47082
    Secondary accession number(s): D6VWH5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 109 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1820 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3