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P47082

- AVT1_YEAST

UniProt

P47082 - AVT1_YEAST

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Protein
Vacuolar amino acid transporter 1
Gene
AVT1, YJR001W, J1409, YJR83.4
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Required for the vacuolar uptake of large neutral amino acids including tyrosine, glutamine, asparagine, isoleucine and leucine. Requires ATP for function.1 Publication

GO - Molecular functioni

  1. L-glutamine transmembrane transporter activity Source: SGD
  2. L-isoleucine transmembrane transporter activity Source: SGD
  3. L-tyrosine transmembrane transporter activity Source: SGD
  4. transporter activity Source: SGD

GO - Biological processi

  1. L-alpha-amino acid transmembrane transport Source: GOC
  2. L-amino acid transport Source: GOC
  3. amino acid transport Source: SGD
  4. branched-chain amino acid transport Source: GOC
  5. cation transport Source: GOC
  6. glutamine transport Source: GOC
  7. neutral amino acid transport Source: GOC
  8. tyrosine transport Source: GOC
  9. vacuolar transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31647-MONOMER.

Protein family/group databases

TCDBi2.A.18.5.2. the amino acid/auxin permease (aaap) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar amino acid transporter 1
Gene namesi
Name:AVT1
Ordered Locus Names:YJR001W
ORF Names:J1409, YJR83.4
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

CYGDiYJR001w.
SGDiS000003761. AVT1.

Subcellular locationi

Vacuole membrane; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 208208Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei209 – 23123Helical; Reviewed prediction
Add
BLAST
Topological domaini232 – 2409Vacuolar Reviewed prediction
Transmembranei241 – 26121Helical; Reviewed prediction
Add
BLAST
Topological domaini262 – 28625Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei287 – 30721Helical; Reviewed prediction
Add
BLAST
Topological domaini308 – 32114Vacuolar Reviewed prediction
Add
BLAST
Transmembranei322 – 34221Helical; Reviewed prediction
Add
BLAST
Topological domaini343 – 3442Cytoplasmic Reviewed prediction
Transmembranei345 – 36521Helical; Reviewed prediction
Add
BLAST
Topological domaini366 – 38924Vacuolar Reviewed prediction
Add
BLAST
Transmembranei390 – 41021Helical; Reviewed prediction
Add
BLAST
Topological domaini411 – 42919Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei430 – 45021Helical; Reviewed prediction
Add
BLAST
Topological domaini451 – 46616Vacuolar Reviewed prediction
Add
BLAST
Transmembranei467 – 48721Helical; Reviewed prediction
Add
BLAST
Topological domaini488 – 51730Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei518 – 53821Helical; Reviewed prediction
Add
BLAST
Topological domaini539 – 5435Vacuolar Reviewed prediction
Transmembranei544 – 56421Helical; Reviewed prediction
Add
BLAST
Topological domaini565 – 58016Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei581 – 60121Helical; Reviewed prediction
Add
BLAST
Topological domaini602 – 6021Vacuolar Reviewed prediction

GO - Cellular componenti

  1. fungal-type vacuole Source: SGD
  2. fungal-type vacuole membrane Source: SGD
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 602602Vacuolar amino acid transporter 1
PRO_0000093834Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei8 – 81Phosphoserine1 Publication
Modified residuei15 – 151Phosphoserine1 Publication
Modified residuei35 – 351Phosphoserine1 Publication
Modified residuei181 – 1811Phosphothreonine2 Publications
Modified residuei187 – 1871Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP47082.
PaxDbiP47082.
PRIDEiP47082.

Expressioni

Gene expression databases

GenevestigatoriP47082.

Interactioni

Protein-protein interaction databases

BioGridi33757. 26 interactions.
DIPiDIP-5709N.
IntActiP47082. 2 interactions.
MINTiMINT-504618.
STRINGi4932.YJR001W.

Structurei

3D structure databases

ProteinModelPortaliP47082.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0814.
GeneTreeiENSGT00490000043380.
HOGENOMiHOG000235065.
KOiK15015.
OMAiKTDMRHP.
OrthoDBiEOG7MD4ZV.

Family and domain databases

InterProiIPR013057. AA_transpt_TM.
[Graphical view]
PfamiPF01490. Aa_trans. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47082-1 [UniParc]FASTAAdd to Basket

« Hide

MPEQEPLSPN GRKRSEVHYI SIPLNRGSAF SPDDSVSQFQ SDGFMTRRQS    50
ILDHPVGSFK GVNSLSRFAT SLRRANSFRN IELNADNERS FFKESNDETY 100
DPDTLAPALD GRRLSVTLNN AGRPRITNLA NNDRVSTASM AIHDDDYGSI 150
QNSTIGDSGS ILRPTASLTE MMSGGAGRRF TNNDMDSIVV KRVEGVDGKV 200
VTLLAGQSTA PQTIFNSINV LIGIGLLALP LGLKYAGWVI GLTMLAIFAL 250
ATFCTAELLS RCLDTDPTLI SYADLGYAAF GTKGRALISA LFTLDLLGSG 300
VSLVILFGDS LNALFPQYST TFFKIVSFFI VTPPVFIPLS VLSNISLLGI 350
LSTTGTVLVI CCCGLYKSSS PGSLVNPMET SMWPIDLKHL CLSIGLLSAC 400
WGGHAVFPNL KTDMRHPDKF KDCLKTTYKI TSVTDIGTAV IGFLMFGNLV 450
KDEITKNVLL TEGYPKFVYG LISALMTIIP IAKTPLNARP IVSVLDVLMN 500
VQHIDEAASA IKRRAAKGLQ VFNRIFINVV FVLIAINFPE FDKIIAFLGA 550
GLCFTICLIL PCWFYLRLCK TTIKPWERVA CHVTICISVV LSTLGVGAAI 600
IS 602
Length:602
Mass (Da):65,346
Last modified:February 1, 1996 - v1
Checksum:iC2F321E76C21C2B4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X87611 Genomic DNA. Translation: CAA60922.1.
Z49501 Genomic DNA. Translation: CAA89523.1.
BK006943 Genomic DNA. Translation: DAA08791.1.
PIRiS55188.
RefSeqiNP_012534.1. NM_001181658.1.

Genome annotation databases

EnsemblFungiiYJR001W; YJR001W; YJR001W.
GeneIDi853457.
KEGGisce:YJR001W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X87611 Genomic DNA. Translation: CAA60922.1 .
Z49501 Genomic DNA. Translation: CAA89523.1 .
BK006943 Genomic DNA. Translation: DAA08791.1 .
PIRi S55188.
RefSeqi NP_012534.1. NM_001181658.1.

3D structure databases

ProteinModelPortali P47082.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33757. 26 interactions.
DIPi DIP-5709N.
IntActi P47082. 2 interactions.
MINTi MINT-504618.
STRINGi 4932.YJR001W.

Protein family/group databases

TCDBi 2.A.18.5.2. the amino acid/auxin permease (aaap) family.

Proteomic databases

MaxQBi P47082.
PaxDbi P47082.
PRIDEi P47082.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YJR001W ; YJR001W ; YJR001W .
GeneIDi 853457.
KEGGi sce:YJR001W.

Organism-specific databases

CYGDi YJR001w.
SGDi S000003761. AVT1.

Phylogenomic databases

eggNOGi COG0814.
GeneTreei ENSGT00490000043380.
HOGENOMi HOG000235065.
KOi K15015.
OMAi KTDMRHP.
OrthoDBi EOG7MD4ZV.

Enzyme and pathway databases

BioCyci YEAST:G3O-31647-MONOMER.

Miscellaneous databases

NextBioi 974029.

Gene expression databases

Genevestigatori P47082.

Family and domain databases

InterProi IPR013057. AA_transpt_TM.
[Graphical view ]
Pfami PF01490. Aa_trans. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "A family of yeast proteins mediating bidirectional vacuolar amino acid transport."
    Russnak R., Konczal D., McIntire S.L.
    J. Biol. Chem. 276:23849-23857(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-181 AND SER-187, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-8 AND SER-35, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-15; THR-181 AND SER-187, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiAVT1_YEAST
AccessioniPrimary (citable) accession number: P47082
Secondary accession number(s): D6VWH5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: May 14, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1820 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3

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