Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P47082 (AVT1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Vacuolar amino acid transporter 1
Gene names
Name:AVT1
Ordered Locus Names:YJR001W
ORF Names:J1409, YJR83.4
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length602 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for the vacuolar uptake of large neutral amino acids including tyrosine, glutamine, asparagine, isoleucine and leucine. Requires ATP for function. Ref.3

Subcellular location

Vacuole membrane; Multi-pass membrane protein Ref.3.

Miscellaneous

Present with 1820 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the amino acid/polyamine transporter 2 family.

Ontologies

Keywords
   Biological processAmino-acid transport
Transport
   Cellular componentMembrane
Vacuole
   DomainTransmembrane
Transmembrane helix
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processL-alpha-amino acid transmembrane transport

Inferred from mutant phenotype Ref.3. Source: GOC

L-amino acid transport

Inferred from mutant phenotype Ref.3. Source: GOC

amino acid transport

Inferred from mutant phenotype Ref.3. Source: SGD

branched-chain amino acid transport

Inferred from mutant phenotype Ref.3. Source: GOC

cation transport

Inferred from mutant phenotype Ref.3. Source: GOC

glutamine transport

Inferred from mutant phenotype Ref.3. Source: GOC

neutral amino acid transport

Inferred from mutant phenotype Ref.3. Source: GOC

tyrosine transport

Inferred from mutant phenotype Ref.3. Source: GOC

vacuolar transport

Inferred from mutant phenotype Ref.3. Source: SGD

   Cellular_componentfungal-type vacuole

Inferred from direct assay Ref.3. Source: SGD

fungal-type vacuole membrane

Inferred from mutant phenotype Ref.3. Source: SGD

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionL-glutamine transmembrane transporter activity

Inferred from mutant phenotype Ref.3. Source: SGD

L-isoleucine transmembrane transporter activity

Inferred from mutant phenotype Ref.3. Source: SGD

L-tyrosine transmembrane transporter activity

Inferred from mutant phenotype Ref.3. Source: SGD

transporter activity

Inferred from sequence or structural similarity Ref.3. Source: SGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 602602Vacuolar amino acid transporter 1
PRO_0000093834

Regions

Topological domain1 – 208208Cytoplasmic Potential
Transmembrane209 – 23123Helical; Potential
Topological domain232 – 2409Vacuolar Potential
Transmembrane241 – 26121Helical; Potential
Topological domain262 – 28625Cytoplasmic Potential
Transmembrane287 – 30721Helical; Potential
Topological domain308 – 32114Vacuolar Potential
Transmembrane322 – 34221Helical; Potential
Topological domain343 – 3442Cytoplasmic Potential
Transmembrane345 – 36521Helical; Potential
Topological domain366 – 38924Vacuolar Potential
Transmembrane390 – 41021Helical; Potential
Topological domain411 – 42919Cytoplasmic Potential
Transmembrane430 – 45021Helical; Potential
Topological domain451 – 46616Vacuolar Potential
Transmembrane467 – 48721Helical; Potential
Topological domain488 – 51730Cytoplasmic Potential
Transmembrane518 – 53821Helical; Potential
Topological domain539 – 5435Vacuolar Potential
Transmembrane544 – 56421Helical; Potential
Topological domain565 – 58016Cytoplasmic Potential
Transmembrane581 – 60121Helical; Potential
Topological domain6021Vacuolar Potential

Amino acid modifications

Modified residue81Phosphoserine Ref.7
Modified residue151Phosphoserine Ref.8
Modified residue351Phosphoserine Ref.7
Modified residue1811Phosphothreonine Ref.6 Ref.8
Modified residue1871Phosphoserine Ref.6 Ref.8

Sequences

Sequence LengthMass (Da)Tools
P47082 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: C2F321E76C21C2B4

FASTA60265,346
        10         20         30         40         50         60 
MPEQEPLSPN GRKRSEVHYI SIPLNRGSAF SPDDSVSQFQ SDGFMTRRQS ILDHPVGSFK 

        70         80         90        100        110        120 
GVNSLSRFAT SLRRANSFRN IELNADNERS FFKESNDETY DPDTLAPALD GRRLSVTLNN 

       130        140        150        160        170        180 
AGRPRITNLA NNDRVSTASM AIHDDDYGSI QNSTIGDSGS ILRPTASLTE MMSGGAGRRF 

       190        200        210        220        230        240 
TNNDMDSIVV KRVEGVDGKV VTLLAGQSTA PQTIFNSINV LIGIGLLALP LGLKYAGWVI 

       250        260        270        280        290        300 
GLTMLAIFAL ATFCTAELLS RCLDTDPTLI SYADLGYAAF GTKGRALISA LFTLDLLGSG 

       310        320        330        340        350        360 
VSLVILFGDS LNALFPQYST TFFKIVSFFI VTPPVFIPLS VLSNISLLGI LSTTGTVLVI 

       370        380        390        400        410        420 
CCCGLYKSSS PGSLVNPMET SMWPIDLKHL CLSIGLLSAC WGGHAVFPNL KTDMRHPDKF 

       430        440        450        460        470        480 
KDCLKTTYKI TSVTDIGTAV IGFLMFGNLV KDEITKNVLL TEGYPKFVYG LISALMTIIP 

       490        500        510        520        530        540 
IAKTPLNARP IVSVLDVLMN VQHIDEAASA IKRRAAKGLQ VFNRIFINVV FVLIAINFPE 

       550        560        570        580        590        600 
FDKIIAFLGA GLCFTICLIL PCWFYLRLCK TTIKPWERVA CHVTICISVV LSTLGVGAAI 


IS 

« Hide

References

« Hide 'large scale' references
[1]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"A family of yeast proteins mediating bidirectional vacuolar amino acid transport."
Russnak R., Konczal D., McIntire S.L.
J. Biol. Chem. 276:23849-23857(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
[6]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-181 AND SER-187, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-8 AND SER-35, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-15; THR-181 AND SER-187, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X87611 Genomic DNA. Translation: CAA60922.1.
Z49501 Genomic DNA. Translation: CAA89523.1.
BK006943 Genomic DNA. Translation: DAA08791.1.
PIRS55188.
RefSeqNP_012534.1. NM_001181658.1.

3D structure databases

ProteinModelPortalP47082.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33757. 26 interactions.
DIPDIP-5709N.
IntActP47082. 2 interactions.
MINTMINT-504618.
STRING4932.YJR001W.

Protein family/group databases

TCDB2.A.18.5.2. the amino acid/auxin permease (aaap) family.

Proteomic databases

MaxQBP47082.
PaxDbP47082.
PRIDEP47082.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJR001W; YJR001W; YJR001W.
GeneID853457.
KEGGsce:YJR001W.

Organism-specific databases

CYGDYJR001w.
SGDS000003761. AVT1.

Phylogenomic databases

eggNOGCOG0814.
GeneTreeENSGT00490000043380.
HOGENOMHOG000235065.
KOK15015.
OMAKTDMRHP.
OrthoDBEOG7MD4ZV.

Enzyme and pathway databases

BioCycYEAST:G3O-31647-MONOMER.

Gene expression databases

GenevestigatorP47082.

Family and domain databases

InterProIPR013057. AA_transpt_TM.
[Graphical view]
PfamPF01490. Aa_trans. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio974029.

Entry information

Entry nameAVT1_YEAST
AccessionPrimary (citable) accession number: P47082
Secondary accession number(s): D6VWH5
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: May 14, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families