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Protein

Vacuolar amino acid transporter 1

Gene

AVT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for the vacuolar uptake of large neutral amino acids including tyrosine, glutamine, asparagine, isoleucine and leucine. Requires ATP for function.1 Publication

Miscellaneous

Present with 1820 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • L-glutamine transmembrane transporter activity Source: SGD
  • L-isoleucine transmembrane transporter activity Source: SGD
  • L-tyrosine transmembrane transporter activity Source: SGD

GO - Biological processi

  • amino acid transmembrane transport Source: SGD
  • amino acid transport Source: SGD
  • proline transport Source: SGD
  • vacuolar transport Source: SGD

Keywordsi

Biological processAmino-acid transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31647-MONOMER
ReactomeiR-SCE-425393 Transport of inorganic cations/anions and amino acids/oligopeptides
R-SCE-888590 GABA synthesis, release, reuptake and degradation

Protein family/group databases

TCDBi2.A.18.5.2 the amino acid/auxin permease (aaap) family

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar amino acid transporter 1
Gene namesi
Name:AVT1
Ordered Locus Names:YJR001W
ORF Names:J1409, YJR83.4
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJR001W
SGDiS000003761 AVT1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 208CytoplasmicSequence analysisAdd BLAST208
Transmembranei209 – 231HelicalSequence analysisAdd BLAST23
Topological domaini232 – 240VacuolarSequence analysis9
Transmembranei241 – 261HelicalSequence analysisAdd BLAST21
Topological domaini262 – 286CytoplasmicSequence analysisAdd BLAST25
Transmembranei287 – 307HelicalSequence analysisAdd BLAST21
Topological domaini308 – 321VacuolarSequence analysisAdd BLAST14
Transmembranei322 – 342HelicalSequence analysisAdd BLAST21
Topological domaini343 – 344CytoplasmicSequence analysis2
Transmembranei345 – 365HelicalSequence analysisAdd BLAST21
Topological domaini366 – 389VacuolarSequence analysisAdd BLAST24
Transmembranei390 – 410HelicalSequence analysisAdd BLAST21
Topological domaini411 – 429CytoplasmicSequence analysisAdd BLAST19
Transmembranei430 – 450HelicalSequence analysisAdd BLAST21
Topological domaini451 – 466VacuolarSequence analysisAdd BLAST16
Transmembranei467 – 487HelicalSequence analysisAdd BLAST21
Topological domaini488 – 517CytoplasmicSequence analysisAdd BLAST30
Transmembranei518 – 538HelicalSequence analysisAdd BLAST21
Topological domaini539 – 543VacuolarSequence analysis5
Transmembranei544 – 564HelicalSequence analysisAdd BLAST21
Topological domaini565 – 580CytoplasmicSequence analysisAdd BLAST16
Transmembranei581 – 601HelicalSequence analysisAdd BLAST21
Topological domaini602VacuolarSequence analysis1

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000938341 – 602Vacuolar amino acid transporter 1Add BLAST602

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei8PhosphoserineCombined sources1
Modified residuei15PhosphoserineCombined sources1
Modified residuei35PhosphoserineCombined sources1
Modified residuei181PhosphothreonineCombined sources1
Modified residuei187PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP47082
PaxDbiP47082
PRIDEiP47082

PTM databases

iPTMnetiP47082

Interactioni

Protein-protein interaction databases

BioGridi33757, 52 interactors
DIPiDIP-5709N
IntActiP47082, 2 interactors
MINTiP47082
STRINGi4932.YJR001W

Structurei

3D structure databases

ProteinModelPortaliP47082
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00490000043380
HOGENOMiHOG000235065
InParanoidiP47082
KOiK15015
OMAiKSDMRHP
OrthoDBiEOG092C28BC

Family and domain databases

InterProiView protein in InterPro
IPR013057 AA_transpt_TM
PfamiView protein in Pfam
PF01490 Aa_trans, 1 hit

Sequencei

Sequence statusi: Complete.

P47082-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPEQEPLSPN GRKRSEVHYI SIPLNRGSAF SPDDSVSQFQ SDGFMTRRQS
60 70 80 90 100
ILDHPVGSFK GVNSLSRFAT SLRRANSFRN IELNADNERS FFKESNDETY
110 120 130 140 150
DPDTLAPALD GRRLSVTLNN AGRPRITNLA NNDRVSTASM AIHDDDYGSI
160 170 180 190 200
QNSTIGDSGS ILRPTASLTE MMSGGAGRRF TNNDMDSIVV KRVEGVDGKV
210 220 230 240 250
VTLLAGQSTA PQTIFNSINV LIGIGLLALP LGLKYAGWVI GLTMLAIFAL
260 270 280 290 300
ATFCTAELLS RCLDTDPTLI SYADLGYAAF GTKGRALISA LFTLDLLGSG
310 320 330 340 350
VSLVILFGDS LNALFPQYST TFFKIVSFFI VTPPVFIPLS VLSNISLLGI
360 370 380 390 400
LSTTGTVLVI CCCGLYKSSS PGSLVNPMET SMWPIDLKHL CLSIGLLSAC
410 420 430 440 450
WGGHAVFPNL KTDMRHPDKF KDCLKTTYKI TSVTDIGTAV IGFLMFGNLV
460 470 480 490 500
KDEITKNVLL TEGYPKFVYG LISALMTIIP IAKTPLNARP IVSVLDVLMN
510 520 530 540 550
VQHIDEAASA IKRRAAKGLQ VFNRIFINVV FVLIAINFPE FDKIIAFLGA
560 570 580 590 600
GLCFTICLIL PCWFYLRLCK TTIKPWERVA CHVTICISVV LSTLGVGAAI

IS
Length:602
Mass (Da):65,346
Last modified:February 1, 1996 - v1
Checksum:iC2F321E76C21C2B4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87611 Genomic DNA Translation: CAA60922.1
Z49501 Genomic DNA Translation: CAA89523.1
BK006943 Genomic DNA Translation: DAA08791.1
PIRiS55188
RefSeqiNP_012534.1, NM_001181658.1

Genome annotation databases

EnsemblFungiiYJR001W; YJR001W; YJR001W
GeneIDi853457
KEGGisce:YJR001W

Similar proteinsi

Entry informationi

Entry nameiAVT1_YEAST
AccessioniPrimary (citable) accession number: P47082
Secondary accession number(s): D6VWH5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: June 20, 2018
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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