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Protein

Vacuolar amino acid transporter 1

Gene

AVT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for the vacuolar uptake of large neutral amino acids including tyrosine, glutamine, asparagine, isoleucine and leucine. Requires ATP for function.1 Publication

GO - Molecular functioni

  • L-glutamine transmembrane transporter activity Source: SGD
  • L-isoleucine transmembrane transporter activity Source: SGD
  • L-tyrosine transmembrane transporter activity Source: SGD

GO - Biological processi

  • amino acid transmembrane transport Source: SGD
  • amino acid transport Source: SGD
  • proline transport Source: SGD
  • vacuolar transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31647-MONOMER.
ReactomeiR-SCE-425393. Transport of inorganic cations/anions and amino acids/oligopeptides.
R-SCE-888590. GABA synthesis, release, reuptake and degradation.

Protein family/group databases

TCDBi2.A.18.5.2. the amino acid/auxin permease (aaap) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar amino acid transporter 1
Gene namesi
Name:AVT1
Ordered Locus Names:YJR001W
ORF Names:J1409, YJR83.4
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJR001W.
SGDiS000003761. AVT1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 208CytoplasmicSequence analysisAdd BLAST208
Transmembranei209 – 231HelicalSequence analysisAdd BLAST23
Topological domaini232 – 240VacuolarSequence analysis9
Transmembranei241 – 261HelicalSequence analysisAdd BLAST21
Topological domaini262 – 286CytoplasmicSequence analysisAdd BLAST25
Transmembranei287 – 307HelicalSequence analysisAdd BLAST21
Topological domaini308 – 321VacuolarSequence analysisAdd BLAST14
Transmembranei322 – 342HelicalSequence analysisAdd BLAST21
Topological domaini343 – 344CytoplasmicSequence analysis2
Transmembranei345 – 365HelicalSequence analysisAdd BLAST21
Topological domaini366 – 389VacuolarSequence analysisAdd BLAST24
Transmembranei390 – 410HelicalSequence analysisAdd BLAST21
Topological domaini411 – 429CytoplasmicSequence analysisAdd BLAST19
Transmembranei430 – 450HelicalSequence analysisAdd BLAST21
Topological domaini451 – 466VacuolarSequence analysisAdd BLAST16
Transmembranei467 – 487HelicalSequence analysisAdd BLAST21
Topological domaini488 – 517CytoplasmicSequence analysisAdd BLAST30
Transmembranei518 – 538HelicalSequence analysisAdd BLAST21
Topological domaini539 – 543VacuolarSequence analysis5
Transmembranei544 – 564HelicalSequence analysisAdd BLAST21
Topological domaini565 – 580CytoplasmicSequence analysisAdd BLAST16
Transmembranei581 – 601HelicalSequence analysisAdd BLAST21
Topological domaini602VacuolarSequence analysis1

GO - Cellular componenti

  • fungal-type vacuole Source: SGD
  • fungal-type vacuole membrane Source: SGD
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000938341 – 602Vacuolar amino acid transporter 1Add BLAST602

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei8PhosphoserineCombined sources1
Modified residuei15PhosphoserineCombined sources1
Modified residuei35PhosphoserineCombined sources1
Modified residuei181PhosphothreonineCombined sources1
Modified residuei187PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP47082.
PRIDEiP47082.

PTM databases

iPTMnetiP47082.

Interactioni

Protein-protein interaction databases

BioGridi33757. 27 interactors.
DIPiDIP-5709N.
IntActiP47082. 2 interactors.
MINTiMINT-504618.

Structurei

3D structure databases

ProteinModelPortaliP47082.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00490000043380.
HOGENOMiHOG000235065.
InParanoidiP47082.
KOiK15015.
OMAiMICLILP.
OrthoDBiEOG092C28BC.

Family and domain databases

InterProiIPR013057. AA_transpt_TM.
[Graphical view]
PfamiPF01490. Aa_trans. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47082-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPEQEPLSPN GRKRSEVHYI SIPLNRGSAF SPDDSVSQFQ SDGFMTRRQS
60 70 80 90 100
ILDHPVGSFK GVNSLSRFAT SLRRANSFRN IELNADNERS FFKESNDETY
110 120 130 140 150
DPDTLAPALD GRRLSVTLNN AGRPRITNLA NNDRVSTASM AIHDDDYGSI
160 170 180 190 200
QNSTIGDSGS ILRPTASLTE MMSGGAGRRF TNNDMDSIVV KRVEGVDGKV
210 220 230 240 250
VTLLAGQSTA PQTIFNSINV LIGIGLLALP LGLKYAGWVI GLTMLAIFAL
260 270 280 290 300
ATFCTAELLS RCLDTDPTLI SYADLGYAAF GTKGRALISA LFTLDLLGSG
310 320 330 340 350
VSLVILFGDS LNALFPQYST TFFKIVSFFI VTPPVFIPLS VLSNISLLGI
360 370 380 390 400
LSTTGTVLVI CCCGLYKSSS PGSLVNPMET SMWPIDLKHL CLSIGLLSAC
410 420 430 440 450
WGGHAVFPNL KTDMRHPDKF KDCLKTTYKI TSVTDIGTAV IGFLMFGNLV
460 470 480 490 500
KDEITKNVLL TEGYPKFVYG LISALMTIIP IAKTPLNARP IVSVLDVLMN
510 520 530 540 550
VQHIDEAASA IKRRAAKGLQ VFNRIFINVV FVLIAINFPE FDKIIAFLGA
560 570 580 590 600
GLCFTICLIL PCWFYLRLCK TTIKPWERVA CHVTICISVV LSTLGVGAAI

IS
Length:602
Mass (Da):65,346
Last modified:February 1, 1996 - v1
Checksum:iC2F321E76C21C2B4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87611 Genomic DNA. Translation: CAA60922.1.
Z49501 Genomic DNA. Translation: CAA89523.1.
BK006943 Genomic DNA. Translation: DAA08791.1.
PIRiS55188.
RefSeqiNP_012534.1. NM_001181658.1.

Genome annotation databases

EnsemblFungiiYJR001W; YJR001W; YJR001W.
GeneIDi853457.
KEGGisce:YJR001W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87611 Genomic DNA. Translation: CAA60922.1.
Z49501 Genomic DNA. Translation: CAA89523.1.
BK006943 Genomic DNA. Translation: DAA08791.1.
PIRiS55188.
RefSeqiNP_012534.1. NM_001181658.1.

3D structure databases

ProteinModelPortaliP47082.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33757. 27 interactors.
DIPiDIP-5709N.
IntActiP47082. 2 interactors.
MINTiMINT-504618.

Protein family/group databases

TCDBi2.A.18.5.2. the amino acid/auxin permease (aaap) family.

PTM databases

iPTMnetiP47082.

Proteomic databases

MaxQBiP47082.
PRIDEiP47082.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJR001W; YJR001W; YJR001W.
GeneIDi853457.
KEGGisce:YJR001W.

Organism-specific databases

EuPathDBiFungiDB:YJR001W.
SGDiS000003761. AVT1.

Phylogenomic databases

GeneTreeiENSGT00490000043380.
HOGENOMiHOG000235065.
InParanoidiP47082.
KOiK15015.
OMAiMICLILP.
OrthoDBiEOG092C28BC.

Enzyme and pathway databases

BioCyciYEAST:G3O-31647-MONOMER.
ReactomeiR-SCE-425393. Transport of inorganic cations/anions and amino acids/oligopeptides.
R-SCE-888590. GABA synthesis, release, reuptake and degradation.

Miscellaneous databases

PROiP47082.

Family and domain databases

InterProiIPR013057. AA_transpt_TM.
[Graphical view]
PfamiPF01490. Aa_trans. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAVT1_YEAST
AccessioniPrimary (citable) accession number: P47082
Secondary accession number(s): D6VWH5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 2, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1820 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.