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Protein

Nucleolar protein 9

Gene

NOP9

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

RNA-binding nucleolar protein required for pre-rRNA processing. Component of the 90S pre-ribosome involved in production of 18S rRNA and assembly of small ribosomal subunit. Component of the pre-40S ribosome required for release from the nucleolus.2 Publications

Miscellaneous

Present with 2930 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • RNA binding Source: SGD

GO - Biological processi

  • endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • ribosomal small subunit export from nucleus Source: SGD

Keywordsi

Biological processRibosome biogenesis, rRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-31486-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleolar protein 9
Alternative name(s):
Pumilio domain-containing protein NOP9
Gene namesi
Name:NOP9
Ordered Locus Names:YJL010C
ORF Names:J1357
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJL010C.
SGDiS000003547. NOP9.

Subcellular locationi

GO - Cellular componenti

  • 90S preribosome Source: SGD
  • nucleolus Source: SGD
  • preribosome, small subunit precursor Source: SGD

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000759391 – 666Nucleolar protein 9Add BLAST666

Proteomic databases

MaxQBiP47077.
PRIDEiP47077.

Interactioni

Subunit structurei

Component of the 90S pre-ribosome. Component of the pre-40S ribosome.1 Publication

Protein-protein interaction databases

BioGridi33747. 228 interactors.
DIPiDIP-5668N.
IntActiP47077. 9 interactors.
MINTiMINT-498143.
STRINGi4932.YJL010C.

Structurei

Secondary structure

1666
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi58 – 73Combined sources16
Helixi79 – 93Combined sources15
Turni94 – 96Combined sources3
Helixi97 – 102Combined sources6
Turni104 – 106Combined sources3
Helixi107 – 116Combined sources10
Helixi119 – 127Combined sources9
Turni128 – 131Combined sources4
Helixi133 – 137Combined sources5
Helixi142 – 159Combined sources18
Helixi178 – 189Combined sources12
Helixi190 – 192Combined sources3
Helixi193 – 197Combined sources5
Helixi202 – 213Combined sources12
Helixi256 – 273Combined sources18
Beta strandi278 – 282Combined sources5
Helixi287 – 295Combined sources9
Turni298 – 300Combined sources3
Helixi301 – 310Combined sources10
Helixi311 – 313Combined sources3
Helixi319 – 324Combined sources6
Beta strandi328 – 330Combined sources3
Helixi333 – 342Combined sources10
Helixi346 – 356Combined sources11
Helixi361 – 371Combined sources11
Helixi373 – 375Combined sources3
Helixi376 – 380Combined sources5
Turni383 – 385Combined sources3
Helixi386 – 396Combined sources11
Helixi399 – 409Combined sources11
Helixi410 – 412Combined sources3
Helixi413 – 419Combined sources7
Helixi421 – 433Combined sources13
Turni434 – 438Combined sources5
Helixi439 – 450Combined sources12
Beta strandi455 – 457Combined sources3
Helixi460 – 465Combined sources6
Helixi467 – 469Combined sources3
Helixi482 – 497Combined sources16
Helixi499 – 511Combined sources13
Helixi514 – 521Combined sources8
Helixi526 – 533Combined sources8
Helixi536 – 538Combined sources3
Helixi541 – 551Combined sources11
Helixi552 – 554Combined sources3
Helixi555 – 559Combined sources5
Helixi564 – 574Combined sources11
Turni575 – 577Combined sources3
Helixi579 – 591Combined sources13
Helixi593 – 597Combined sources5
Helixi600 – 608Combined sources9
Helixi611 – 616Combined sources6
Helixi618 – 632Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5SVDX-ray2.10A/B46-645[»]
5WTXX-ray3.07A1-666[»]
5WTYX-ray2.79A/B53-634[»]
ProteinModelPortaliP47077.
SMRiP47077.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 435PUM-HDAdd BLAST413
Repeati92 – 127Pumilio 1Add BLAST36
Repeati128 – 163Pumilio 2Add BLAST36
Repeati188 – 223Pumilio 3Add BLAST36
Repeati286 – 326Pumilio 4Add BLAST41
Repeati334 – 368Pumilio 5Add BLAST35
Repeati369 – 407Pumilio 6Add BLAST39
Repeati511 – 548Pumilio 7Add BLAST38
Repeati549 – 587Pumilio 8Add BLAST39

Sequence similaritiesi

Belongs to the NOP9 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00390000004964.
InParanoidiP47077.
KOiK14790.
OMAiFLRDQTS.
OrthoDBiEOG092C1FH2.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiView protein in InterPro
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR001313. Pumilio_RNA-bd_rpt.
PfamiView protein in Pfam
PF00806. PUF. 4 hits.
SMARTiView protein in SMART
SM00025. Pumilio. 8 hits.
SUPFAMiSSF48371. SSF48371. 3 hits.

Sequencei

Sequence statusi: Complete.

P47077-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKTKTRGRR HQDKQRKDEF EPSSNSAKEH IQQEESTYND EAEIKETQPQ
60 70 80 90 100
MFFGVLDREE LEYFKQAEST LQLDAFEAPE EKFQFVTSII EEAKGKELKL
110 120 130 140 150
VTSQITSKLM ERVILECDET QLKDIFQSFN GVFFGLSCHK YASHVLETLF
160 170 180 190 200
VRSAALVERE LLTPSFDNNE KEGPYVTMEN MFLFMLNELK PHLKTMMNHQ
210 220 230 240 250
YASHVLRLLI LILSSKTLPN STKANSTLRS KKSKIARKMI DIKDNDDFNK
260 270 280 290 300
VYQTPESFKS ELRDIITTLY KGFTNGAESR SDISQSTITK FREYSVDKVA
310 320 330 340 350
SPVIQLIIQV EGIFDRDRSF WRLVFNTADE KDPKEESFLE YLLSDPVGSH
360 370 380 390 400
FLENVIGSAR LKYVERLYRL YMKDRIVKLA KRDTTGAFVV RALLEHLKEK
410 420 430 440 450
DVKQILDAVV PELSMLLNSN MDFGTAIINA SNKQGGYLRD DVIAQLIQKY
460 470 480 490 500
YPEKSDAKNI LESCLLLSAS TLGNTRDDWP TAEERRRSVF LEQLIDYDDK
510 520 530 540 550
FLNITIDSML ALPEERLIQM CYHGVFSHVV EHVLQTTRVD IIKRKMLLNI
560 570 580 590 600
LSKESVNLAC NVYGSHIMDK LWEFTAKLTL YKERIARALV LETEKVKNSI
610 620 630 640 650
YGRQVWKNWK LELYVRKMWD WKKLIKEQEF EIFPNSKPLQ PKPEKHSRER
660
NNSKEGSAFK KQKHYR
Length:666
Mass (Da):77,722
Last modified:February 1, 1996 - v1
Checksum:iF6F8B3CD74DB2AB3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49285 Genomic DNA. Translation: CAA89301.1.
BK006943 Genomic DNA. Translation: DAA08783.1.
PIRiS56781.
RefSeqiNP_012524.3. NM_001181444.3.

Genome annotation databases

EnsemblFungiiYJL010C; YJL010C; YJL010C.
GeneIDi853445.
KEGGisce:YJL010C.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiNOP9_YEAST
AccessioniPrimary (citable) accession number: P47077
Secondary accession number(s): D6VWG7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: July 5, 2017
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names