Nucleolar protein 9 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P47077 (NOP9_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 93. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Nucleolar protein 9

Alternative name(s):
Pumilio domain-containing protein NOP9

Gene names

Name:	NOP9
Ordered Locus Names:	YJL010C
ORF Names:	J1357

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	666 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	RNA-binding nucleolar protein required for pre-rRNA processing. Component of the 90S pre-ribosome involved in production of 18S rRNA and assembly of small ribosomal subunit. Component of the pre-40S ribosome required for release from the nucleolus. Ref.5 Ref.6
Subunit structure	Component of the 90S pre-ribosome. Component of the pre-40S ribosome. Ref.6
Subcellular location	Nucleus › nucleolus Ref.3 Ref.6.
Miscellaneous	Present with 2930 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the NOP9 family. Contains 1 PUM-HD domain. Contains 8 pumilio repeats.

Ontologies

Keywords
Biological process	Ribosome biogenesis rRNA processing
Cellular component	Nucleus
Domain	Repeat
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype Ref.6. Source: SGD endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype Ref.6. Source: SGD endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype Ref.6. Source: SGD ribosomal small subunit export from nucleus Inferred from mutant phenotype Ref.6. Source: SGD
Cellular_component	90S preribosome Inferred from direct assay Ref.6. Source: SGD nucleolus Inferred from direct assay Ref.3 PubMed 14690591 Ref.6. Source: SGD preribosome, small subunit precursor Inferred from direct assay Ref.6. Source: SGD
Molecular_function	RNA binding Inferred from direct assay Ref.6. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 666

666

Nucleolar protein 9

PRO_0000075939

Regions

Domain

23 – 435

413

PUM-HD

Repeat

92 – 127

Pumilio 1

Repeat

128 – 163

Pumilio 2

Repeat

188 – 223

Pumilio 3

Repeat

286 – 326

Pumilio 4

Repeat

334 – 368

Pumilio 5

Repeat

369 – 407

Pumilio 6

Repeat

511 – 548

Pumilio 7

Repeat

549 – 587

Pumilio 8

Sequences

Sequence

Length

Mass (Da)

Tools

P47077 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: F6F8B3CD74DB2AB3
Show »

FASTA

666

77,722

        10         20         30         40         50         60 
MGKTKTRGRR HQDKQRKDEF EPSSNSAKEH IQQEESTYND EAEIKETQPQ MFFGVLDREE 

        70         80         90        100        110        120 
LEYFKQAEST LQLDAFEAPE EKFQFVTSII EEAKGKELKL VTSQITSKLM ERVILECDET 

       130        140        150        160        170        180 
QLKDIFQSFN GVFFGLSCHK YASHVLETLF VRSAALVERE LLTPSFDNNE KEGPYVTMEN 

       190        200        210        220        230        240 
MFLFMLNELK PHLKTMMNHQ YASHVLRLLI LILSSKTLPN STKANSTLRS KKSKIARKMI 

       250        260        270        280        290        300 
DIKDNDDFNK VYQTPESFKS ELRDIITTLY KGFTNGAESR SDISQSTITK FREYSVDKVA 

       310        320        330        340        350        360 
SPVIQLIIQV EGIFDRDRSF WRLVFNTADE KDPKEESFLE YLLSDPVGSH FLENVIGSAR 

       370        380        390        400        410        420 
LKYVERLYRL YMKDRIVKLA KRDTTGAFVV RALLEHLKEK DVKQILDAVV PELSMLLNSN 

       430        440        450        460        470        480 
MDFGTAIINA SNKQGGYLRD DVIAQLIQKY YPEKSDAKNI LESCLLLSAS TLGNTRDDWP 

       490        500        510        520        530        540 
TAEERRRSVF LEQLIDYDDK FLNITIDSML ALPEERLIQM CYHGVFSHVV EHVLQTTRVD 

       550        560        570        580        590        600 
IIKRKMLLNI LSKESVNLAC NVYGSHIMDK LWEFTAKLTL YKERIARALV LETEKVKNSI 

       610        620        630        640        650        660 
YGRQVWKNWK LELYVRKMWD WKKLIKEQEF EIFPNSKPLQ PKPEKHSRER NNSKEGSAFK 


KQKHYR

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"The budding yeast rRNA and ribosome biosynthesis (RRB) regulon contains over 200 genes." Wade C.H., Umbarger M.A., McAlear M.A. Yeast 23:293-306(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Nop9 is an RNA binding protein present in pre-40S ribosomes and required for 18S rRNA synthesis in yeast." Thomson E., Rappsilber J., Tollervey D. RNA 13:2165-2174(2007) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE 90S PRE-RIBOSOME, IDENTIFICATION IN THE PRE-40S RIBOSOME, FUNCTION, RNA-BINDING, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z49285 Genomic DNA. Translation: CAA89301.1. BK006943 Genomic DNA. Translation: DAA08783.1.
PIR	S56781.
RefSeq	NP_012524.3. NM_001181444.3.
3D structure databases
ProteinModelPortal	P47077.
SMR	P47077. Positions 88-213, 278-311, 545-571.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5668N.
IntAct	P47077. 6 interactions.
MINT	MINT-498143.
STRING	4932.YJL010C.
Proteomic databases
PaxDb	P47077.
PeptideAtlas	P47077.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJL010C; YJL010C; YJL010C.
GeneID	853445.
KEGG	sce:YJL005W. sce:YJL010C.
Organism-specific databases
CYGD	YJL010c.
SGD	S000003547. NOP9.
Phylogenomic databases
eggNOG	NOG276912.
GeneTree	ENSGT00390000004964.
KO	K01768. K14790.
OMA	ERDLMVH.
OrthoDB	EOG4JQ75V.
Gene expression databases
Genevestigator	P47077.
GermOnline	YJL010C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.25.10.10. 1 hit.
InterPro	IPR011989. ARM-like. IPR016024. ARM-type_fold. IPR001313. Pumilio_RNA-bd_rpt. [Graphical view]
Pfam	PF00806. PUF. 4 hits. [Graphical view]
SMART	SM00025. Pumilio. 8 hits. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 1 hit.
PROSITE	PS50302. PUM. False negative. PS50303. PUM_HD. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	974001.

Entry information

Entry name

NOP9_YEAST

Accession

Primary (citable) accession number: P47077
Secondary accession number(s): D6VWG7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	May 1, 2013
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents