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Protein

Nucleolar protein 9

Gene

NOP9

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

RNA-binding nucleolar protein required for pre-rRNA processing. Component of the 90S pre-ribosome involved in production of 18S rRNA and assembly of small ribosomal subunit. Component of the pre-40S ribosome required for release from the nucleolus.2 Publications

GO - Molecular functioni

  • RNA binding Source: SGD

GO - Biological processi

  • endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • ribosomal small subunit export from nucleus Source: SGD
Complete GO annotation...

Keywords - Biological processi

Ribosome biogenesis, rRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-31486-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleolar protein 9
Alternative name(s):
Pumilio domain-containing protein NOP9
Gene namesi
Name:NOP9
Ordered Locus Names:YJL010C
ORF Names:J1357
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJL010C.
SGDiS000003547. NOP9.

Subcellular locationi

GO - Cellular componenti

  • 90S preribosome Source: SGD
  • nucleolus Source: SGD
  • preribosome, small subunit precursor Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 666666Nucleolar protein 9PRO_0000075939Add
BLAST

Proteomic databases

MaxQBiP47077.

Interactioni

Subunit structurei

Component of the 90S pre-ribosome. Component of the pre-40S ribosome.1 Publication

Protein-protein interaction databases

BioGridi33747. 71 interactions.
DIPiDIP-5668N.
IntActiP47077. 9 interactions.
MINTiMINT-498143.

Structurei

3D structure databases

ProteinModelPortaliP47077.
SMRiP47077. Positions 278-310.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini23 – 435413PUM-HDAdd
BLAST
Repeati92 – 12736Pumilio 1Add
BLAST
Repeati128 – 16336Pumilio 2Add
BLAST
Repeati188 – 22336Pumilio 3Add
BLAST
Repeati286 – 32641Pumilio 4Add
BLAST
Repeati334 – 36835Pumilio 5Add
BLAST
Repeati369 – 40739Pumilio 6Add
BLAST
Repeati511 – 54838Pumilio 7Add
BLAST
Repeati549 – 58739Pumilio 8Add
BLAST

Sequence similaritiesi

Belongs to the NOP9 family.Curated
Contains 1 PUM-HD domain.Curated
Contains 8 pumilio repeats.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00390000004964.
InParanoidiP47077.
KOiK14790.
OMAiTVACHRC.
OrthoDBiEOG092C1FH2.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR001313. Pumilio_RNA-bd_rpt.
[Graphical view]
PfamiPF00806. PUF. 4 hits.
[Graphical view]
SMARTiSM00025. Pumilio. 8 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 3 hits.

Sequencei

Sequence statusi: Complete.

P47077-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKTKTRGRR HQDKQRKDEF EPSSNSAKEH IQQEESTYND EAEIKETQPQ
60 70 80 90 100
MFFGVLDREE LEYFKQAEST LQLDAFEAPE EKFQFVTSII EEAKGKELKL
110 120 130 140 150
VTSQITSKLM ERVILECDET QLKDIFQSFN GVFFGLSCHK YASHVLETLF
160 170 180 190 200
VRSAALVERE LLTPSFDNNE KEGPYVTMEN MFLFMLNELK PHLKTMMNHQ
210 220 230 240 250
YASHVLRLLI LILSSKTLPN STKANSTLRS KKSKIARKMI DIKDNDDFNK
260 270 280 290 300
VYQTPESFKS ELRDIITTLY KGFTNGAESR SDISQSTITK FREYSVDKVA
310 320 330 340 350
SPVIQLIIQV EGIFDRDRSF WRLVFNTADE KDPKEESFLE YLLSDPVGSH
360 370 380 390 400
FLENVIGSAR LKYVERLYRL YMKDRIVKLA KRDTTGAFVV RALLEHLKEK
410 420 430 440 450
DVKQILDAVV PELSMLLNSN MDFGTAIINA SNKQGGYLRD DVIAQLIQKY
460 470 480 490 500
YPEKSDAKNI LESCLLLSAS TLGNTRDDWP TAEERRRSVF LEQLIDYDDK
510 520 530 540 550
FLNITIDSML ALPEERLIQM CYHGVFSHVV EHVLQTTRVD IIKRKMLLNI
560 570 580 590 600
LSKESVNLAC NVYGSHIMDK LWEFTAKLTL YKERIARALV LETEKVKNSI
610 620 630 640 650
YGRQVWKNWK LELYVRKMWD WKKLIKEQEF EIFPNSKPLQ PKPEKHSRER
660
NNSKEGSAFK KQKHYR
Length:666
Mass (Da):77,722
Last modified:February 1, 1996 - v1
Checksum:iF6F8B3CD74DB2AB3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49285 Genomic DNA. Translation: CAA89301.1.
BK006943 Genomic DNA. Translation: DAA08783.1.
PIRiS56781.
RefSeqiNP_012524.3. NM_001181444.3.

Genome annotation databases

EnsemblFungiiYJL010C; YJL010C; YJL010C.
GeneIDi853445.
KEGGisce:YJL010C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49285 Genomic DNA. Translation: CAA89301.1.
BK006943 Genomic DNA. Translation: DAA08783.1.
PIRiS56781.
RefSeqiNP_012524.3. NM_001181444.3.

3D structure databases

ProteinModelPortaliP47077.
SMRiP47077. Positions 278-310.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33747. 71 interactions.
DIPiDIP-5668N.
IntActiP47077. 9 interactions.
MINTiMINT-498143.

Proteomic databases

MaxQBiP47077.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJL010C; YJL010C; YJL010C.
GeneIDi853445.
KEGGisce:YJL010C.

Organism-specific databases

EuPathDBiFungiDB:YJL010C.
SGDiS000003547. NOP9.

Phylogenomic databases

GeneTreeiENSGT00390000004964.
InParanoidiP47077.
KOiK14790.
OMAiTVACHRC.
OrthoDBiEOG092C1FH2.

Enzyme and pathway databases

BioCyciYEAST:G3O-31486-MONOMER.

Miscellaneous databases

PROiP47077.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR001313. Pumilio_RNA-bd_rpt.
[Graphical view]
PfamiPF00806. PUF. 4 hits.
[Graphical view]
SMARTiSM00025. Pumilio. 8 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 3 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiNOP9_YEAST
AccessioniPrimary (citable) accession number: P47077
Secondary accession number(s): D6VWG7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: September 7, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2930 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.