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Protein

Spindle assembly checkpoint component MAD3

Gene

MAD3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the spindle assembly checkpoint which is a feedback control that prevents cells with incompletely assembled spindles from leaving mitosis. Component of the mitotic checkpoint complex (MCC) which inhibits the ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) by preventing its activation by CDC20.1 Publication

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • distributive segregation Source: SGD
  • mitotic DNA integrity checkpoint Source: SGD
  • mitotic nuclear division Source: UniProtKB-KW
  • mitotic spindle assembly checkpoint Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-31488-MONOMER.
ReactomeiR-SCE-141430. Inactivation of APC/C via direct inhibition of the APC/C complex.

Names & Taxonomyi

Protein namesi
Recommended name:
Spindle assembly checkpoint component MAD3
Alternative name(s):
Mitotic MAD3 protein
Gene namesi
Name:MAD3
Ordered Locus Names:YJL013C
ORF Names:J1341
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJL013C.
SGDiS000003550. MAD3.

Subcellular locationi

GO - Cellular componenti

  • mitotic checkpoint complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi156 – 1594GIGS → AAAA: Abolishes interaction with CDC20. Benomyl-sensitive phenotype. 1 Publication
Mutagenesisi382 – 3821E → K: Abolishes interaction with BUB3. Benomyl-sensitive phenotype. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 515515Spindle assembly checkpoint component MAD3PRO_0000084549Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei268 – 2681PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP47074.

PTM databases

iPTMnetiP47074.

Interactioni

Subunit structurei

Component of the mitotic checkpoint complex (MCC) which consists of MAD2, MAD3, BUB3 and CDC20. Interacts with CDC20 and BUB3.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BUB3P264499EBI-10369,EBI-3830
CDC20P263098EBI-10369,EBI-4212
MAD2P409583EBI-10369,EBI-10362

Protein-protein interaction databases

BioGridi33742. 94 interactions.
DIPiDIP-1268N.
IntActiP47074. 9 interactions.
MINTiMINT-396546.

Structurei

Secondary structure

1
515
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi356 – 3605Combined sources
Helixi362 – 3654Combined sources
Helixi381 – 3877Combined sources
Turni388 – 3903Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2I3TX-ray2.80B/D/F/H354-400[»]
ProteinModelPortaliP47074.
SMRiP47074. Positions 19-227, 354-395.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP47074.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini67 – 228162BUB1 N-terminalPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 BUB1 N-terminal domain.PROSITE-ProRule annotation
To yeast protein kinase BUB1 in its non-catalytic N-terminal domain.Curated

Phylogenomic databases

GeneTreeiENSGT00520000055622.
InParanoidiP47074.
KOiK06680.
OMAiFEHAKEN.
OrthoDBiEOG092C0DAL.

Family and domain databases

InterProiIPR015661. Bub1/Mad3.
IPR013212. Mad3/Bub1_I.
IPR012572. Mad3/Bub1_II.
[Graphical view]
PANTHERiPTHR14030. PTHR14030. 1 hit.
PfamiPF08311. Mad3_BUB1_I. 1 hit.
PF08171. Mad3_BUB1_II. 1 hit.
[Graphical view]
SMARTiSM00777. Mad3_BUB1_I. 1 hit.
[Graphical view]
PROSITEiPS51489. BUB1_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47074-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAYAKKRIS YMPSSPSQNV INFEEIETQK ENILPLKEGR SAAALSKAIH
60 70 80 90 100
QPLVEINQVK SSFEQRLIDE LPALSDPITL YLEYIKWLNN AYPQGGNSKQ
110 120 130 140 150
SGMLTLLERC LSHLKDLERY RNDVRFLKIW FWYIELFTRN SFMESRDIFM
160 170 180 190 200
YMLRNGIGSE LASFYEEFTN LLIQKEKFQY AVKILQLGIK NKARPNKVLE
210 220 230 240 250
DRLNHLLREL GENNIQLGNE ISMDSLESTV LGKTRSEFVN RLELANQNGT
260 270 280 290 300
SSDVNLTKNN VFVDGEESDV ELFETPNRGV YRDGWENFDL KAERNKENNL
310 320 330 340 350
RISLLEANTN LGELKQHEML SQKKRPYDEK LPIFRDSIGR SDPVYQMINT
360 370 380 390 400
KDQKPEKIDC NFKLIYCEDE ESKGGRLEFS LEEVLAISRN VYKRVRTNRK
410 420 430 440 450
HPREANLGQE ESANQKEAEA QSKRPKISRK ALVSKSLTPS NQGRMFSGEE
460 470 480 490 500
YINCPMTPKG RSTETSDIIS AVKPRQLTPI LEMRESNSFS QSKNSEIISD
510
DDKSSSSFIS YPPQR
Length:515
Mass (Da):59,521
Last modified:February 1, 1996 - v1
Checksum:i36550249D81BB6D1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49288 Genomic DNA. Translation: CAA89304.1.
BK006943 Genomic DNA. Translation: DAA08780.1.
PIRiS56784.
RefSeqiNP_012521.3. NM_001181447.3.

Genome annotation databases

EnsemblFungiiYJL013C; YJL013C; YJL013C.
GeneIDi853439.
KEGGisce:YJL013C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49288 Genomic DNA. Translation: CAA89304.1.
BK006943 Genomic DNA. Translation: DAA08780.1.
PIRiS56784.
RefSeqiNP_012521.3. NM_001181447.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2I3TX-ray2.80B/D/F/H354-400[»]
ProteinModelPortaliP47074.
SMRiP47074. Positions 19-227, 354-395.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33742. 94 interactions.
DIPiDIP-1268N.
IntActiP47074. 9 interactions.
MINTiMINT-396546.

PTM databases

iPTMnetiP47074.

Proteomic databases

MaxQBiP47074.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJL013C; YJL013C; YJL013C.
GeneIDi853439.
KEGGisce:YJL013C.

Organism-specific databases

EuPathDBiFungiDB:YJL013C.
SGDiS000003550. MAD3.

Phylogenomic databases

GeneTreeiENSGT00520000055622.
InParanoidiP47074.
KOiK06680.
OMAiFEHAKEN.
OrthoDBiEOG092C0DAL.

Enzyme and pathway databases

BioCyciYEAST:G3O-31488-MONOMER.
ReactomeiR-SCE-141430. Inactivation of APC/C via direct inhibition of the APC/C complex.

Miscellaneous databases

EvolutionaryTraceiP47074.
PROiP47074.

Family and domain databases

InterProiIPR015661. Bub1/Mad3.
IPR013212. Mad3/Bub1_I.
IPR012572. Mad3/Bub1_II.
[Graphical view]
PANTHERiPTHR14030. PTHR14030. 1 hit.
PfamiPF08311. Mad3_BUB1_I. 1 hit.
PF08171. Mad3_BUB1_II. 1 hit.
[Graphical view]
SMARTiSM00777. Mad3_BUB1_I. 1 hit.
[Graphical view]
PROSITEiPS51489. BUB1_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMAD3_YEAST
AccessioniPrimary (citable) accession number: P47074
Secondary accession number(s): D6VWG4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: September 7, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3170 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.