Spindle assembly checkpoint component MAD3 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Contribute

Send feedback

Read comments (?) or add your own

P47074 (MAD3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 105. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Spindle assembly checkpoint component MAD3

Alternative name(s):
Mitotic MAD3 protein

Gene names

Name:	MAD3
Ordered Locus Names:	YJL013C
ORF Names:	J1341

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	515 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the spindle assembly checkpoint which is a feedback control that prevents cells with incompletely assembled spindles from leaving mitosis. Component of the mitotic checkpoint complex (MCC) which inhibits the ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) by preventing its activation by CDC20. Ref.3
Subunit structure	Component of the mitotic checkpoint complex (MCC) which consists of MAD2, MAD3, BUB3 and CDC20. Interacts with CDC20 and BUB3. Ref.3 Ref.4 Ref.6
Subcellular location	Nucleus.
Miscellaneous	Present with 3170 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 BUB1 N-terminal domain. To yeast protein kinase BUB1 in its non-catalytic N-terminal domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Nucleus
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell division Inferred from electronic annotation. Source: UniProtKB-KW distributive segregation Inferred from mutant phenotype PubMed 15951820. Source: SGD mitosis Inferred from electronic annotation. Source: UniProtKB-KW mitotic DNA integrity checkpoint Inferred from genetic interaction PubMed 16651657. Source: SGD mitotic spindle assembly checkpoint Inferred from genetic interaction Ref.3. Source: SGD
Cellular_component	mitotic checkpoint complex Inferred from direct assay Ref.4. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
BUB3	P26449	9	EBI-10369,EBI-3830
CDC20	P26309	9	EBI-10369,EBI-4212
MAD2	P40958	3	EBI-10369,EBI-10362

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 515

515

Spindle assembly checkpoint component MAD3

PRO_0000084549

Regions

Domain

67 – 228

162

BUB1 N-terminal

Amino acid modifications

Modified residue

Phosphoserine Ref.9

Modified residue

303

Phosphoserine Ref.8

Modified residue

478

Phosphothreonine Ref.7

Modified residue

488

Phosphoserine Ref.9

Experimental info

Mutagenesis

156 – 159

GIGS → AAAA: Abolishes interaction with CDC20. Benomyl-sensitive phenotype. Ref.3

Mutagenesis

382

E → K: Abolishes interaction with BUB3. Benomyl-sensitive phenotype. Ref.3

Secondary structure

515

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P47074 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: 36550249D81BB6D1
Show »

FASTA

515

59,521

        10         20         30         40         50         60 
MKAYAKKRIS YMPSSPSQNV INFEEIETQK ENILPLKEGR SAAALSKAIH QPLVEINQVK 

        70         80         90        100        110        120 
SSFEQRLIDE LPALSDPITL YLEYIKWLNN AYPQGGNSKQ SGMLTLLERC LSHLKDLERY 

       130        140        150        160        170        180 
RNDVRFLKIW FWYIELFTRN SFMESRDIFM YMLRNGIGSE LASFYEEFTN LLIQKEKFQY 

       190        200        210        220        230        240 
AVKILQLGIK NKARPNKVLE DRLNHLLREL GENNIQLGNE ISMDSLESTV LGKTRSEFVN 

       250        260        270        280        290        300 
RLELANQNGT SSDVNLTKNN VFVDGEESDV ELFETPNRGV YRDGWENFDL KAERNKENNL 

       310        320        330        340        350        360 
RISLLEANTN LGELKQHEML SQKKRPYDEK LPIFRDSIGR SDPVYQMINT KDQKPEKIDC 

       370        380        390        400        410        420 
NFKLIYCEDE ESKGGRLEFS LEEVLAISRN VYKRVRTNRK HPREANLGQE ESANQKEAEA 

       430        440        450        460        470        480 
QSKRPKISRK ALVSKSLTPS NQGRMFSGEE YINCPMTPKG RSTETSDIIS AVKPRQLTPI 

       490        500        510 
LEMRESNSFS QSKNSEIISD DDKSSSSFIS YPPQR

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"MAD3 encodes a novel component of the spindle checkpoint which interacts with Bub3p, Cdc20p, and Mad2p." Hardwick K.G., Johnston R.C., Smith D.L., Murray A.W. J. Cell Biol. 148:871-882(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH CDC20 AND BUB3, MUTAGENESIS OF 156-GLY--SER-159 AND GLU-382.
[4]	"Bub3 interaction with Mad2, Mad3 and Cdc20 is mediated by WD40 repeats and does not require intact kinetochores." Fraschini R., Beretta A., Sironi L., Musacchio A., Lucchini G., Piatti S. EMBO J. 20:6648-6659(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH BUB3, IDENTIFICATION IN THE MCC COMPLEX.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Two complexes of spindle checkpoint proteins containing Cdc20 and Mad2 assemble during mitosis independently of the kinetochore in Saccharomyces cerevisiae." Poddar A., Stukenberg P.T., Burke D.J. Eukaryot. Cell 4:867-878(2005) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE MCC COMPLEX.
[7]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-478, MASS SPECTROMETRY. Strain: YAL6B.
[8]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-303, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-15 AND SER-488, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z49288 Genomic DNA. Translation: CAA89304.1.
BK006943 Genomic DNA. Translation: DAA08780.1.

PIR

S56784.

RefSeq

NP_012521.3. NM_001181447.3.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2I3T	X-ray	2.80	B/D/F/H	354-400	[»]

ProteinModelPortal

P47074.

SMR

P47074. Positions 19-227, 354-395.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-1268N.

IntAct

P47074. 9 interactions.

MINT

MINT-396546.

STRING

4932.YJL013C.

Proteomic databases

PaxDb

P47074.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YJL013C; YJL013C; YJL013C.

GeneID

853439.

KEGG

sce:YJL010C.
sce:YJL013C.

Organism-specific databases

CYGD

YJL013c.

SGD

S000003550. MAD3.

Phylogenomic databases

eggNOG

NOG320292.

GeneTree

ENSGT00520000055622.

K06680.
K14790.

OrthoDB

EOG45QMPQ.

Gene expression databases

Genevestigator

P47074.

GermOnline

YJL013C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR015661. Bub1/Mad3.
IPR012572. Mad3-like.
IPR013212. Mad3_BUB1_I.
[Graphical view]

PANTHER

PTHR14030. PTHR14030. 1 hit.

Pfam

PF08311. Mad3_BUB1_I. 1 hit.
PF08171. Mad3_BUB1_II. 1 hit.
[Graphical view]

SMART

SM00777. Mad3_BUB1_I. 1 hit.
[Graphical view]

PROSITE

PS51489. BUB1_N. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P47074.

NextBio

973988.

Entry information

Entry name

MAD3_YEAST

Accession

Primary (citable) accession number: P47074
Secondary accession number(s): D6VWG4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	May 1, 2013
This is version 105 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents