MAD3

Functionⁱ

Component of the spindle assembly checkpoint which is a feedback control that prevents cells with incompletely assembled spindles from leaving mitosis. Component of the mitotic checkpoint complex (MCC) which inhibits the ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) by preventing its activation by CDC20.1 Publication

Miscellaneous

Present with 3170 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functionⁱ

protein kinase activity Source: GO_Central

Complete GO annotation...

GO - Biological processⁱ

cell division Source: UniProtKB-KW
distributive segregation
Source: SGD
Inferred from mutant phenotypeⁱ
- 15951820
meiotic sister chromatid cohesion, centromeric Source: GO_Central
mitotic DNA integrity checkpoint
Source: SGD
Inferred from genetic interactionⁱ
- 16651657
mitotic spindle assembly checkpoint
Source: SGD
Inferred from mutant phenotypeⁱ
- 10704439

Complete GO annotation...

Keywordsⁱ

Biological process	Cell cycle, Cell division, Mitosis

Biological process

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

BioCycⁱ	YEAST:G3O-31488-MONOMER.
Reactomeⁱ	R-SCE-141430. Inactivation of APC/C via direct inhibition of the APC/C complex.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Spindle assembly checkpoint component MAD3 Alternative name(s): Mitotic MAD3 protein
Gene namesⁱ	Name:MAD3 Ordered Locus Names:YJL013C ORF Names:J1341
Organismⁱ	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifierⁱ	559292 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae
Proteomesⁱ	UP000002311 Componentⁱ: Chromosome X

Organism-specific databases

EuPathDBⁱ	FungiDB:YJL013C.
Saccharomyces Genome Database More...SGDⁱ	S000003550. MAD3.

Subcellular locationⁱ

Nucleus

GO - Cellular componentⁱ

condensed nuclear chromosome kinetochore Source: GO_Central
mitotic checkpoint complex
Source: SGD
Inferred from direct assayⁱ
- 11726501

Complete GO annotation...

Keywords - Cellular componentⁱ

Nucleus

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Mutagenesisⁱ	156 – 159	GIGS → AAAA: Abolishes interaction with CDC20. Benomyl-sensitive phenotype. 1 Publication		4
Mutagenesisⁱ	382	E → K: Abolishes interaction with BUB3. Benomyl-sensitive phenotype. 1 Publication		1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000084549	1 – 515	Spindle assembly checkpoint component MAD3Add BLAST		515

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	268	PhosphoserineCombined sources		1

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P47074.
PRIDEⁱ	P47074.

PTM databases

iPTMnetⁱ	P47074.

iPTMnetⁱ

P47074.

Interactionⁱ

Subunit structureⁱ

Component of the mitotic checkpoint complex (MCC) which consists of MAD2, MAD3, BUB3 and CDC20. Interacts with CDC20 and BUB3.3 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct
BUB3	P26449	9	EBI-10369,EBI-3830
CDC20	P26309	8	EBI-10369,EBI-4212
MAD2	P40958	3	EBI-10369,EBI-10362

Show more details

Protein-protein interaction databases

BioGridⁱ	33742. 140 interactors.
Database of interacting proteins More...DIPⁱ	DIP-1268N.
IntActⁱ	P47074. 9 interactors.
Molecular INTeraction database More...MINTⁱ	MINT-396546.
STRINGⁱ	4932.YJL013C.

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Show more details

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	356 – 360	Combined sources	5
Helixⁱ	362 – 365	Combined sources	4
Helixⁱ	381 – 387	Combined sources	7
Turnⁱ	388 – 390	Combined sources	3

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	2I3T	X-ray	2.80	B/D/F/H	354-400	[»]
ProteinModelPortalⁱ	P47074.
SMRⁱ	P47074.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ

P47074.

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	67 – 228	BUB1 N-terminalPROSITE-ProRule annotationAdd BLAST		162

Sequence similaritiesⁱ

To yeast protein kinase BUB1 in its non-catalytic N-terminal domain.Curated

Phylogenomic databases

Ensembl GeneTree More...GeneTreeⁱ	ENSGT00520000055622.
InParanoidⁱ	P47074.
KEGG Orthology (KO) More...KOⁱ	K06680.
Database of Orthologous Groups More...OrthoDBⁱ	EOG092C0DAL.

Family and domain databases

Gene3Dⁱ	1.25.40.10. 1 hit.
InterProⁱ	View protein in InterPro IPR015661. Bub1/Mad3. IPR013212. Mad3/Bub1_I. IPR012572. Mad3/Bub1_II. IPR011990. TPR-like_helical_dom.
PANTHERⁱ	PTHR14030. PTHR14030. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF08311. Mad3_BUB1_I. 1 hit. PF08171. Mad3_BUB1_II. 1 hit.
SMARTⁱ	View protein in SMART SM00777. Mad3_BUB1_I. 1 hit.
PROSITEⁱ	View protein in PROSITE PS51489. BUB1_N. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

P47074-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MKAYAKKRIS YMPSSPSQNV INFEEIETQK ENILPLKEGR SAAALSKAIH 
        60         70         80         90        100
QPLVEINQVK SSFEQRLIDE LPALSDPITL YLEYIKWLNN AYPQGGNSKQ 
       110        120        130        140        150
SGMLTLLERC LSHLKDLERY RNDVRFLKIW FWYIELFTRN SFMESRDIFM 
       160        170        180        190        200
YMLRNGIGSE LASFYEEFTN LLIQKEKFQY AVKILQLGIK NKARPNKVLE 
       210        220        230        240        250
DRLNHLLREL GENNIQLGNE ISMDSLESTV LGKTRSEFVN RLELANQNGT 
       260        270        280        290        300
SSDVNLTKNN VFVDGEESDV ELFETPNRGV YRDGWENFDL KAERNKENNL 
       310        320        330        340        350
RISLLEANTN LGELKQHEML SQKKRPYDEK LPIFRDSIGR SDPVYQMINT 
       360        370        380        390        400
KDQKPEKIDC NFKLIYCEDE ESKGGRLEFS LEEVLAISRN VYKRVRTNRK 
       410        420        430        440        450
HPREANLGQE ESANQKEAEA QSKRPKISRK ALVSKSLTPS NQGRMFSGEE 
       460        470        480        490        500
YINCPMTPKG RSTETSDIIS AVKPRQLTPI LEMRESNSFS QSKNSEIISD 
       510 
DDKSSSSFIS YPPQR

Length:515

Mass (Da):59,521

Last modified:February 1, 1996 - v1

Checksum:ⁱ36550249D81BB6D1

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	Z49288 Genomic DNA. Translation: CAA89304.1. BK006943 Genomic DNA. Translation: DAA08780.1.
PIRⁱ	S56784.
NCBI Reference Sequences More...RefSeqⁱ	NP_012521.3. NM_001181447.3.

Genome annotation databases

EnsemblFungiⁱ	YJL013C; YJL013C; YJL013C.
GeneIDⁱ	853439.
KEGGⁱ	sce:YJL013C.

Similar proteinsⁱ

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:

100%	UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%	UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%	UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	Z49288 Genomic DNA. Translation: CAA89304.1. BK006943 Genomic DNA. Translation: DAA08780.1.
PIRⁱ	S56784.
NCBI Reference Sequences More...RefSeqⁱ	NP_012521.3. NM_001181447.3.

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	2I3T	X-ray	2.80	B/D/F/H	354-400	[»]
ProteinModelPortalⁱ	P47074.
SMRⁱ	P47074.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	33742. 140 interactors.
Database of interacting proteins More...DIPⁱ	DIP-1268N.
IntActⁱ	P47074. 9 interactors.
Molecular INTeraction database More...MINTⁱ	MINT-396546.
STRINGⁱ	4932.YJL013C.

PTM databases

iPTMnetⁱ	P47074.

iPTMnetⁱ

P47074.

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P47074.
PRIDEⁱ	P47074.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblFungiⁱ	YJL013C; YJL013C; YJL013C.
GeneIDⁱ	853439.
KEGGⁱ	sce:YJL013C.

Organism-specific databases

EuPathDBⁱ	FungiDB:YJL013C.
Saccharomyces Genome Database More...SGDⁱ	S000003550. MAD3.

Phylogenomic databases

Ensembl GeneTree More...GeneTreeⁱ	ENSGT00520000055622.
InParanoidⁱ	P47074.
KEGG Orthology (KO) More...KOⁱ	K06680.
Database of Orthologous Groups More...OrthoDBⁱ	EOG092C0DAL.

Enzyme and pathway databases

BioCycⁱ	YEAST:G3O-31488-MONOMER.
Reactomeⁱ	R-SCE-141430. Inactivation of APC/C via direct inhibition of the APC/C complex.

Miscellaneous databases

EvolutionaryTraceⁱ	P47074.
Protein Ontology More...PROⁱ	PR:P47074.

Family and domain databases

Gene3Dⁱ	1.25.40.10. 1 hit.
InterProⁱ	View protein in InterPro IPR015661. Bub1/Mad3. IPR013212. Mad3/Bub1_I. IPR012572. Mad3/Bub1_II. IPR011990. TPR-like_helical_dom.
PANTHERⁱ	PTHR14030. PTHR14030. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF08311. Mad3_BUB1_I. 1 hit. PF08171. Mad3_BUB1_II. 1 hit.
SMARTⁱ	View protein in SMART SM00777. Mad3_BUB1_I. 1 hit.
PROSITEⁱ	View protein in PROSITE PS51489. BUB1_N. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	MAD3_YEAST
Accessionⁱ	P47074Primary (citable) accession number: P47074 Secondary accession number(s): D6VWG4
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
	Last sequence update:	February 1, 1996
	Last modified:	July 5, 2017
	This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Fungal Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

PDB cross-references
Index of Protein Data Bank (PDB) cross-references
Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
Yeast chromosome X
Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P47074 (MAD3_YEAST)

Your basket is currently empty.

Spindle assembly checkpoint component MAD3

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Proteomic databases

PTM databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequenceⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ