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P47068

- BBC1_YEAST

UniProt

P47068 - BBC1_YEAST

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Protein

Myosin tail region-interacting protein MTI1

Gene

BBC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in the regulation of actin cytoskeleton.1 Publication

GO - Molecular functioni

  1. myosin I binding Source: UniProtKB

GO - Biological processi

  1. actin cytoskeleton organization Source: SGD
  2. cytoskeleton organization Source: UniProtKB
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-31492-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin tail region-interacting protein MTI1
Alternative name(s):
Protein BBC1
Gene namesi
Name:BBC1
Synonyms:MTI1
Ordered Locus Names:YJL020C
ORF Names:J1286, J1305, YJL021C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

CYGDiYJL020c.
SGDiS000003557. BBC1.

Subcellular locationi

Cytoplasmcytoskeletonactin patch 1 Publication
Note: Colocalizes with cortical actin patches.

GO - Cellular componenti

  1. actin cortical patch Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11571157Myosin tail region-interacting protein MTI1PRO_0000064839Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei103 – 1031Phosphoserine4 Publications
Modified residuei158 – 1581Phosphoserine3 Publications
Modified residuei166 – 1661Phosphoserine3 Publications
Modified residuei565 – 5651Phosphoserine3 Publications
Modified residuei621 – 6211Phosphoserine2 Publications
Modified residuei631 – 6311Phosphoserine3 Publications
Modified residuei634 – 6341Phosphoserine2 Publications
Modified residuei636 – 6361Phosphothreonine1 Publication
Modified residuei638 – 6381Phosphoserine3 Publications
Modified residuei647 – 6471Phosphoserine2 Publications
Modified residuei850 – 8501Phosphothreonine1 Publication
Modified residuei889 – 8891Phosphoserine1 Publication
Modified residuei894 – 8941Phosphothreonine2 Publications
Modified residuei895 – 8951Phosphothreonine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP47068.
PaxDbiP47068.
PeptideAtlasiP47068.

Expressioni

Gene expression databases

GenevestigatoriP47068.

Interactioni

Subunit structurei

Binds to the SH3 domains of the type I myosins MYO3 and MYO5.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
AIM21P405638EBI-3437,EBI-25376
APP1P539333EBI-3437,EBI-28798
MYO3P360065EBI-3437,EBI-11670
MYO5Q044396EBI-3437,EBI-11687
YPR091CQ068332EBI-3437,EBI-37290

Protein-protein interaction databases

BioGridi33738. 128 interactions.
DIPiDIP-2614N.
DIP-6279N.
IntActiP47068. 71 interactions.
MINTiMINT-553243.

Structurei

Secondary structure

1
1157
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi6 – 149Combined sources
Beta strandi21 – 233Combined sources
Beta strandi31 – 377Combined sources
Beta strandi39 – 4810Combined sources
Beta strandi54 – 607Combined sources
Helixi61 – 633Combined sources
Beta strandi64 – 663Combined sources

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1TG0X-ray0.97A1-68[»]
1WDXX-ray2.50A/B/C/D1-68[»]
1ZUKX-ray1.90A/B1-68[»]
2DYFNMR-B796-802[»]
ProteinModelPortaliP47068.
SMRiP47068. Positions 3-68.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP47068.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 6965SH3PROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili234 – 30168Sequence AnalysisAdd
BLAST
Coiled coili356 – 43075Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi278 – 412135Glu-richAdd
BLAST
Compositional biasi674 – 830157Pro-richAdd
BLAST

Sequence similaritiesi

Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, SH3 domain

Phylogenomic databases

eggNOGiNOG12793.
InParanoidiP47068.
OMAiRTESHEE.
OrthoDBiEOG7FBRS6.

Family and domain databases

InterProiIPR001452. SH3_domain.
[Graphical view]
PfamiPF00018. SH3_1. 1 hit.
[Graphical view]
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47068-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSEPEVPFKV VAQFPYKSDY EDDLNFEKDQ EIIVTSVEDA EWYFGEYQDS
60 70 80 90 100
NGDVIEGIFP KSFVAVQGSE VGKEAESSPN TGSTEQRTIQ PEVEQKDLPE
110 120 130 140 150
PISPETKKET LSGPVPVPAA TVPVPAATVP VPAATAVSAQ VQHDSSSGNG
160 170 180 190 200
ERKVPMDSPK LKARLSMFNQ DITEQVPLPK STHLDLENIP VKKTIVADAP
210 220 230 240 250
KYYVPPGIPT NDTSNLERKK SLKENEKKIV PEPINRAQVE SGRIETENDQ
260 270 280 290 300
LKKDLPQMSL KERIALLQEQ QRLQAAREEE LLRKKAKLEQ EHERSAVNKN
310 320 330 340 350
EPYTETEEAE ENEKTEPKPE FTPETEHNEE PQMELLAHKE ITKTSREADE
360 370 380 390 400
GTNDIEKEQF LDEYTKENQK VEESQADEAR GENVAEESEI GYGHEDREGD
410 420 430 440 450
NDEEKEEEDS EENRRAALRE RMAKLSGASR FGAPVGFNPF GMASGVGNKP
460 470 480 490 500
SEEPKKKQHK EKEEEEPEQL QELPRAIPVM PFVDPSSNPF FRKSNLSEKN
510 520 530 540 550
QPTETKTLDP HATTEHEQKQ EHGTHAYHNL AAVDNAHPEY SDHDSDEDTD
560 570 580 590 600
DHEFEDANDG LRKHSMVEQA FQIGNNESEN VNSGEKIYPQ EPPISHRTAE
610 620 630 640 650
VSHDIENSSQ NTTGNVLPVS SPQTRVARNG SINSLTKSIS GENRRKSINE
660 670 680 690 700
YHDTVSTNSS ALTETAQDIS MAAPAAPVLS KVSHPEDKVP PHPVPSAPSA
710 720 730 740 750
PPVPSAPSVP SAPPVPPAPP ALSAPSVPPV PPVPPVSSAP PALSAPSIPP
760 770 780 790 800
VPPTPPAPPA PPAPLALPKH NEVEEHVKSS APLPPVSEEY HPMPNTAPPL
810 820 830 840 850
PRAPPVPPAT FEFDSEPTAT HSHTAPSPPP HQNVTASTPS MMSTQQRVPT
860 870 880 890 900
SVLSGAEKES RTLPPHVPSL TNRPVDSFHE SDTTPKVASI RRSTTHDVGE
910 920 930 940 950
ISNNVKIEFN AQERWWINKS APPAISNLKL NFLMEIDDHF ISKRLHQKWV
960 970 980 990 1000
VRDFYFLFEN YSQLRFSLTF NSTSPEKTVT TLQERFPSPV ETQSARILDE
1010 1020 1030 1040 1050
YAQRFNAKVV EKSHSLINSH IGAKNFVSQI VSEFKDEVIQ PIGARTFGAT
1060 1070 1080 1090 1100
ILSYKPEEGI EQLMKSLQKI KPGDILVIRK AKFEAHKKIG KNEIINVGMD
1110 1120 1130 1140 1150
SAAPYSSVVT DYDFTKNKFR VIENHEGKII QNSYKLSHMK SGKLKVFRIV

ARGYVGW
Length:1,157
Mass (Da):128,297
Last modified:January 17, 2003 - v2
Checksum:i88A5899B89CCE8ED
GO

Sequence cautioni

The sequence CAA89311.1 differs from that shown. Reason: Frameshift at positions 729 and 732.
The sequence CAA89312.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z49295 Genomic DNA. Translation: CAA89311.1. Frameshift.
Z49296 Genomic DNA. Translation: CAA89312.1. Different initiation.
AF373805 Genomic DNA. Translation: AAL57239.1.
BK006943 Genomic DNA. Translation: DAA08776.1.
PIRiS56791.
S56792.
RefSeqiNP_012514.2. NM_001181454.1.

Genome annotation databases

EnsemblFungiiYJL020C; YJL020C; YJL020C.
GeneIDi853433.
KEGGisce:YJL020C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z49295 Genomic DNA. Translation: CAA89311.1 . Frameshift.
Z49296 Genomic DNA. Translation: CAA89312.1 . Different initiation.
AF373805 Genomic DNA. Translation: AAL57239.1 .
BK006943 Genomic DNA. Translation: DAA08776.1 .
PIRi S56791.
S56792.
RefSeqi NP_012514.2. NM_001181454.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1TG0 X-ray 0.97 A 1-68 [» ]
1WDX X-ray 2.50 A/B/C/D 1-68 [» ]
1ZUK X-ray 1.90 A/B 1-68 [» ]
2DYF NMR - B 796-802 [» ]
ProteinModelPortali P47068.
SMRi P47068. Positions 3-68.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33738. 128 interactions.
DIPi DIP-2614N.
DIP-6279N.
IntActi P47068. 71 interactions.
MINTi MINT-553243.

Proteomic databases

MaxQBi P47068.
PaxDbi P47068.
PeptideAtlasi P47068.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YJL020C ; YJL020C ; YJL020C .
GeneIDi 853433.
KEGGi sce:YJL020C.

Organism-specific databases

CYGDi YJL020c.
SGDi S000003557. BBC1.

Phylogenomic databases

eggNOGi NOG12793.
InParanoidi P47068.
OMAi RTESHEE.
OrthoDBi EOG7FBRS6.

Enzyme and pathway databases

BioCyci YEAST:G3O-31492-MONOMER.

Miscellaneous databases

EvolutionaryTracei P47068.
NextBioi 973976.
PROi P47068.

Gene expression databases

Genevestigatori P47068.

Family and domain databases

InterProi IPR001452. SH3_domain.
[Graphical view ]
Pfami PF00018. SH3_1. 1 hit.
[Graphical view ]
SMARTi SM00326. SH3. 1 hit.
[Graphical view ]
SUPFAMi SSF50044. SSF50044. 1 hit.
PROSITEi PS50002. SH3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "The novel adaptor protein, Mti1p, and Vrp1p, a homolog of Wiskott-Aldrich syndrome protein-interacting protein (WIP), may antagonistically regulate type I myosins in Saccharomyces cerevisiae."
    Mochida J., Yamamoto T., Fujimura-Kamada K., Tanaka K.
    Genetics 160:923-934(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 699-765, FUNCTION, SUBUNIT, SUBCELLULAR LOCATION.
  4. Pohl T.M., Aljinovic G.
    Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 775-1157.
  5. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
    Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
    Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-631 AND SER-638, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: YAL6B.
  6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-103; SER-158; SER-166; SER-565; SER-621; SER-631; SER-634; SER-638; THR-894 AND THR-895, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  7. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-103; SER-158 AND SER-889, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-103; SER-166; SER-565 AND SER-647, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-103; SER-158; SER-166; SER-565; SER-621; SER-631; SER-634; THR-636; SER-638; SER-647; THR-850; THR-894 AND THR-895, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiBBC1_YEAST
AccessioniPrimary (citable) accession number: P47068
Secondary accession number(s): D6VWG0, P47067, Q8X1F4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: January 17, 2003
Last modified: October 29, 2014
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3