Myosin tail region-interacting protein MTI1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Contribute

Send feedback

Read comments (?) or add your own

P47068 (BBC1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 113. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Myosin tail region-interacting protein MTI1

Alternative name(s):
Protein BBC1

Gene names

Name:	BBC1
Synonyms:	MTI1
Ordered Locus Names:	YJL020C
ORF Names:	J1286, J1305, YJL021C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	1157 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the regulation of actin cytoskeleton. Ref.3
Subunit structure	Binds to the SH3 domains of the type I myosins MYO3 and MYO5. Ref.3
Subcellular location	Cytoplasm › cytoskeleton › actin patch. Note: Colocalizes with cortical actin patches. Ref.3
Sequence similarities	Contains 1 SH3 domain.
Sequence caution	The sequence CAA89311.1 differs from that shown. Reason: Frameshift at positions 729 and 732. The sequence CAA89312.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Domain	Coiled coil SH3 domain
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	actin cytoskeleton organization Inferred from physical interaction Ref.3. Source: SGD
Cellular_component	actin cortical patch Inferred from direct assay PubMed 11743162 Ref.3. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
LAS17	Q12446	8	EBI-3437,EBI-10022
MYO3	P36006	3	EBI-3437,EBI-11670
MYO5	Q04439	3	EBI-3437,EBI-11687

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1157

1157

Myosin tail region-interacting protein MTI1

PRO_0000064839

Regions

Domain

5 – 69

SH3

Coiled coil

234 – 301

Potential

Coiled coil

356 – 430

Potential

Compositional bias

278 – 412

135

Glu-rich

Compositional bias

674 – 830

157

Pro-rich

Amino acid modifications

Modified residue

Phosphothreonine Ref.8 Ref.9

Modified residue

103

Phosphoserine Ref.6 Ref.7 Ref.8 Ref.9

Modified residue

106

Phosphothreonine Ref.9

Modified residue

158

Phosphoserine Ref.6 Ref.7 Ref.8 Ref.9

Modified residue

166

Phosphoserine Ref.5 Ref.6 Ref.8 Ref.9

Modified residue

221

Phosphoserine Ref.7

Modified residue

565

Phosphoserine Ref.6 Ref.9

Modified residue

602

Phosphoserine Ref.9

Modified residue

620

Phosphoserine Ref.8

Modified residue

621

Phosphoserine Ref.5 Ref.6 Ref.9

Modified residue

624

Phosphothreonine Ref.9

Modified residue

631

Phosphoserine Ref.5 Ref.6 Ref.9

Modified residue

634

Phosphoserine Ref.6 Ref.9

Modified residue

638

Phosphoserine Ref.5 Ref.6

Modified residue

647

Phosphoserine Ref.9

Modified residue

651

Phosphotyrosine Ref.9

Modified residue

654

Phosphothreonine Ref.9

Modified residue

850

Phosphothreonine Ref.9

Modified residue

854

Phosphoserine Ref.9

Modified residue

889

Phosphoserine Ref.7

Modified residue

893

Phosphoserine Ref.8 Ref.9

Modified residue

894

Phosphothreonine Ref.6 Ref.7 Ref.8 Ref.9

Modified residue

895

Phosphothreonine Ref.5 Ref.6 Ref.7 Ref.8 Ref.9

Secondary structure

1157

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P47068 [UniParc].

Last modified January 17, 2003. Version 2.
Checksum: 88A5899B89CCE8ED
Show »

FASTA

1,157

128,297

        10         20         30         40         50         60 
MSEPEVPFKV VAQFPYKSDY EDDLNFEKDQ EIIVTSVEDA EWYFGEYQDS NGDVIEGIFP 

        70         80         90        100        110        120 
KSFVAVQGSE VGKEAESSPN TGSTEQRTIQ PEVEQKDLPE PISPETKKET LSGPVPVPAA 

       130        140        150        160        170        180 
TVPVPAATVP VPAATAVSAQ VQHDSSSGNG ERKVPMDSPK LKARLSMFNQ DITEQVPLPK 

       190        200        210        220        230        240 
STHLDLENIP VKKTIVADAP KYYVPPGIPT NDTSNLERKK SLKENEKKIV PEPINRAQVE 

       250        260        270        280        290        300 
SGRIETENDQ LKKDLPQMSL KERIALLQEQ QRLQAAREEE LLRKKAKLEQ EHERSAVNKN 

       310        320        330        340        350        360 
EPYTETEEAE ENEKTEPKPE FTPETEHNEE PQMELLAHKE ITKTSREADE GTNDIEKEQF 

       370        380        390        400        410        420 
LDEYTKENQK VEESQADEAR GENVAEESEI GYGHEDREGD NDEEKEEEDS EENRRAALRE 

       430        440        450        460        470        480 
RMAKLSGASR FGAPVGFNPF GMASGVGNKP SEEPKKKQHK EKEEEEPEQL QELPRAIPVM 

       490        500        510        520        530        540 
PFVDPSSNPF FRKSNLSEKN QPTETKTLDP HATTEHEQKQ EHGTHAYHNL AAVDNAHPEY 

       550        560        570        580        590        600 
SDHDSDEDTD DHEFEDANDG LRKHSMVEQA FQIGNNESEN VNSGEKIYPQ EPPISHRTAE 

       610        620        630        640        650        660 
VSHDIENSSQ NTTGNVLPVS SPQTRVARNG SINSLTKSIS GENRRKSINE YHDTVSTNSS 

       670        680        690        700        710        720 
ALTETAQDIS MAAPAAPVLS KVSHPEDKVP PHPVPSAPSA PPVPSAPSVP SAPPVPPAPP 

       730        740        750        760        770        780 
ALSAPSVPPV PPVPPVSSAP PALSAPSIPP VPPTPPAPPA PPAPLALPKH NEVEEHVKSS 

       790        800        810        820        830        840 
APLPPVSEEY HPMPNTAPPL PRAPPVPPAT FEFDSEPTAT HSHTAPSPPP HQNVTASTPS 

       850        860        870        880        890        900 
MMSTQQRVPT SVLSGAEKES RTLPPHVPSL TNRPVDSFHE SDTTPKVASI RRSTTHDVGE 

       910        920        930        940        950        960 
ISNNVKIEFN AQERWWINKS APPAISNLKL NFLMEIDDHF ISKRLHQKWV VRDFYFLFEN 

       970        980        990       1000       1010       1020 
YSQLRFSLTF NSTSPEKTVT TLQERFPSPV ETQSARILDE YAQRFNAKVV EKSHSLINSH 

      1030       1040       1050       1060       1070       1080 
IGAKNFVSQI VSEFKDEVIQ PIGARTFGAT ILSYKPEEGI EQLMKSLQKI KPGDILVIRK 

      1090       1100       1110       1120       1130       1140 
AKFEAHKKIG KNEIINVGMD SAAPYSSVVT DYDFTKNKFR VIENHEGKII QNSYKLSHMK 

      1150 
SGKLKVFRIV ARGYVGW

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"The novel adaptor protein, Mti1p, and Vrp1p, a homolog of Wiskott-Aldrich syndrome protein-interacting protein (WIP), may antagonistically regulate type I myosins in Saccharomyces cerevisiae." Mochida J., Yamamoto T., Fujimura-Kamada K., Tanaka K. Genetics 160:923-934(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 699-765, FUNCTION, SUBUNIT, SUBCELLULAR LOCATION.
[4]	Pohl T.M., Aljinovic G. Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 775-1157.
[5]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-166; SER-621; SER-631; SER-638 AND THR-895, MASS SPECTROMETRY. Strain: YAL6B.
[6]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-103; SER-158; SER-166; SER-565; SER-621; SER-631; SER-634; SER-638; THR-894 AND THR-895, MASS SPECTROMETRY. Strain: ADR376.
[7]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-103; SER-158; SER-221; SER-889; THR-894 AND THR-895, MASS SPECTROMETRY.
[8]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-81; SER-103; SER-158; SER-166; SER-620; SER-893; THR-894 AND THR-895, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-81; SER-103; THR-106; SER-158; SER-166; SER-565; SER-602; SER-621; THR-624; SER-631; SER-634; SER-647; TYR-651; THR-654; THR-850; SER-854; SER-893; THR-894 AND THR-895, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z49295 Genomic DNA. Translation: CAA89311.1. Frameshift.
Z49296 Genomic DNA. Translation: CAA89312.1. Different initiation.
AF373805 Genomic DNA. Translation: AAL57239.1.
BK006943 Genomic DNA. Translation: DAA08776.1.

PIR

S56791.
S56792.

RefSeq

NP_012514.2. NM_001181454.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1TG0	X-ray	0.97	A	1-68	[»]
1WDX	X-ray	2.50	A/B/C/D	1-68	[»]
1ZUK	X-ray	1.90	A/B	1-68	[»]
2DYF	NMR	-	B	796-802	[»]

ProteinModelPortal

P47068.

SMR

P47068. Positions 3-68.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-2614N.
DIP-6279N.

IntAct

P47068. 27 interactions.

MINT

MINT-553243.

Proteomic databases

PaxDb

P47068.

PeptideAtlas

P47068.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YJL020C; YJL020C; YJL020C.

GeneID

853433.

KEGG

sce:YJL020C.

Organism-specific databases

CYGD

YJL020c.

SGD

S000003557. BBC1.

Phylogenomic databases

eggNOG

NOG12793.

GeneTree

ENSGT00700000105185.

OMA

NARFQGH.

OrthoDB

EOG4M952C.

Gene expression databases

Genevestigator

P47068.

GermOnline

YJL020C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR001452. SH3_domain.
[Graphical view]

Pfam

PF00018. SH3_1. 1 hit.
[Graphical view]

SMART

SM00326. SH3. 1 hit.
[Graphical view]

SUPFAM

SSF50044. SH3. 1 hit.

PROSITE

PS50002. SH3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P47068.

NextBio

973976.

Entry information

Entry name

BBC1_YEAST

Accession

Primary (citable) accession number: P47068
Secondary accession number(s): D6VWG0, P47067, Q8X1F4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	January 17, 2003
Last modified:	April 3, 2013
This is version 113 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents