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P47064

- AP3S_YEAST

UniProt

P47064 - AP3S_YEAST

Protein

AP-3 complex subunit sigma

Gene

APS3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to the vacuole. Required for the transport via the ALP pathway, which directs the transport of the cargo proteins PHO8 and VAM3 to the vacuole.3 Publications

    GO - Molecular functioni

    1. protein transporter activity Source: InterPro

    GO - Biological processi

    1. Golgi to vacuole transport Source: SGD
    2. protein targeting to vacuole Source: SGD

    Keywords - Biological processi

    Protein transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31494-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    AP-3 complex subunit sigma
    Alternative name(s):
    AP-3 complex sigma3A subunit
    Adaptor-related protein complex 3 subunit sigma
    Clathrin-associated/assembly/adaptor protein, small 3
    Sigma-adaptin 3A
    Sigma3-adaptin
    Gene namesi
    Name:APS3
    Synonyms:YKS7
    Ordered Locus Names:YJL024C
    ORF Names:J1274
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome X

    Organism-specific databases

    CYGDiYJL024c.
    SGDiS000003561. APS3.

    Subcellular locationi

    Golgi apparatus. Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side
    Note: Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.

    GO - Cellular componenti

    1. AP-3 adaptor complex Source: SGD
    2. cytoplasmic vesicle membrane Source: UniProtKB-SubCell
    3. Golgi apparatus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasmic vesicle, Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 194194AP-3 complex subunit sigmaPRO_0000193822Add
    BLAST

    Proteomic databases

    MaxQBiP47064.
    PaxDbiP47064.

    Expressioni

    Gene expression databases

    GenevestigatoriP47064.

    Interactioni

    Subunit structurei

    Adaptor protein complex 3 (AP-3) is a heterotetramer composed of 2 large adaptins (APL5 and APL6), a medium adaptin (APM3) and a small adaptin (APS3).2 Publications

    Protein-protein interaction databases

    BioGridi33735. 67 interactions.
    DIPiDIP-3978N.
    IntActiP47064. 3 interactions.
    MINTiMINT-565998.
    STRINGi4932.YJL024C.

    Structurei

    3D structure databases

    ProteinModelPortaliP47064.
    SMRiP47064. Positions 1-150.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG5030.
    GeneTreeiENSGT00550000074761.
    HOGENOMiHOG000185228.
    KOiK12399.
    OMAiLSEMIIG.
    OrthoDBiEOG79KPTF.

    Family and domain databases

    InterProiIPR016635. AP_complex_ssu.
    IPR022775. AP_mu_sigma_su.
    IPR027155. APS3.
    IPR000804. Clathrin_sm-chain_CS.
    IPR011012. Longin-like_dom.
    [Graphical view]
    PANTHERiPTHR11753. PTHR11753. 1 hit.
    PTHR11753:SF2. PTHR11753:SF2. 1 hit.
    PfamiPF01217. Clat_adaptor_s. 1 hit.
    [Graphical view]
    PIRSFiPIRSF015588. AP_complex_sigma. 1 hit.
    SUPFAMiSSF64356. SSF64356. 1 hit.
    PROSITEiPS00989. CLAT_ADAPTOR_S. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P47064-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIHAVLIFNK KCQPRLVKFY TPVDLPKQKL LLEQVYELIS QRNSDFQSSF    50
    LVTPPSLLLS NENNNDEVNN EDIQIIYKNY ATLYFTFIVD DQESELAILD 100
    LIQTFVESLD RCFTEVNELD LIFNWQTLES VLEEIVQGGM VIETNVNRIV 150
    ASVDELNKAA ESTDSKIGRL TSTGFGSALQ AFAQGGFAQW ATGQ 194
    Length:194
    Mass (Da):21,938
    Last modified:November 1, 1995 - v1
    Checksum:i688D81AEA77B7555
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U31448 Genomic DNA. Translation: AAA92051.1.
    Z49299 Genomic DNA. Translation: CAA89315.1.
    BK006943 Genomic DNA. Translation: DAA08774.1.
    PIRiS56796.
    RefSeqiNP_012510.3. NM_001181458.3.

    Genome annotation databases

    EnsemblFungiiYJL024C; YJL024C; YJL024C.
    GeneIDi853429.
    KEGGisce:YJL024C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U31448 Genomic DNA. Translation: AAA92051.1 .
    Z49299 Genomic DNA. Translation: CAA89315.1 .
    BK006943 Genomic DNA. Translation: DAA08774.1 .
    PIRi S56796.
    RefSeqi NP_012510.3. NM_001181458.3.

    3D structure databases

    ProteinModelPortali P47064.
    SMRi P47064. Positions 1-150.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33735. 67 interactions.
    DIPi DIP-3978N.
    IntActi P47064. 3 interactions.
    MINTi MINT-565998.
    STRINGi 4932.YJL024C.

    Proteomic databases

    MaxQBi P47064.
    PaxDbi P47064.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YJL024C ; YJL024C ; YJL024C .
    GeneIDi 853429.
    KEGGi sce:YJL024C.

    Organism-specific databases

    CYGDi YJL024c.
    SGDi S000003561. APS3.

    Phylogenomic databases

    eggNOGi COG5030.
    GeneTreei ENSGT00550000074761.
    HOGENOMi HOG000185228.
    KOi K12399.
    OMAi LSEMIIG.
    OrthoDBi EOG79KPTF.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31494-MONOMER.

    Miscellaneous databases

    NextBioi 973968.

    Gene expression databases

    Genevestigatori P47064.

    Family and domain databases

    InterProi IPR016635. AP_complex_ssu.
    IPR022775. AP_mu_sigma_su.
    IPR027155. APS3.
    IPR000804. Clathrin_sm-chain_CS.
    IPR011012. Longin-like_dom.
    [Graphical view ]
    PANTHERi PTHR11753. PTHR11753. 1 hit.
    PTHR11753:SF2. PTHR11753:SF2. 1 hit.
    Pfami PF01217. Clat_adaptor_s. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF015588. AP_complex_sigma. 1 hit.
    SUPFAMi SSF64356. SSF64356. 1 hit.
    PROSITEi PS00989. CLAT_ADAPTOR_S. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Suppressors of YCK-encoded yeast casein kinase 1 deficiency define the four subunits of a novel clathrin AP-like complex."
      Panek H.R., Stepp J.D., Engle H.M., Marks K.M., Tan P.K., Lemmon S.K., Robinson L.C.
      EMBO J. 16:4194-4204(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], IDENTIFICATION IN THE AP-3 COMPLEX, FUNCTION OF THE AP-3 COMPLEX.
    2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "The AP-3 adaptor complex is essential for cargo-selective transport to the yeast vacuole."
      Cowles C.R., Odorizzi G., Payne G.S., Emr S.D.
      Cell 91:109-118(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE AP-3 COMPLEX, FUNCTION OF THE AP-3 COMPLEX.
    5. "Formation of AP-3 transport intermediates requires Vps41 function."
      Rehling P., Darsow T., Katzmann D.J., Emr S.D.
      Nat. Cell Biol. 1:346-353(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, FUNCTION OF THE AP-3 COMPLEX.
    6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiAP3S_YEAST
    AccessioniPrimary (citable) accession number: P47064
    Secondary accession number(s): D6VWF8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 114 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1240 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3