P47064 (AP3S_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
AP-3 complex subunit sigma

Alternative name(s):
AP-3 complex sigma3A subunit
Adapter-related protein complex 3 sigma subunit
Clathrin-associated/assembly/adapter protein, small 3
Sigma-adaptin 3A
Sigma3-adaptin

Gene names

Name:	APS3
Synonyms:	YKS7
Ordered Locus Names:	YJL024C
ORF Names:	J1274

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	194 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Part of the AP-3 complex, an adapter-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to the vacuole. Required for the transport via the ALP pathway, which directs the transport of the cargo proteins PHO8 and VAM3 to the vacuole. Ref.1 Ref.4 Ref.5
Subunit structure	Adapter protein complex 3 (AP-3) is a heterotetramer composed of 2 large adaptins (APL5 and APL6), a medium adaptin (APM3) and a small adaptin (APS3). Ref.1 Ref.4
Subcellular location	Golgi apparatus. Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Note: Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex. Ref.5 Ref.6
Miscellaneous	Present with 1240 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the adaptor complexes small subunit family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasmic vesicle Golgi apparatus Membrane
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Golgi to vacuole transport Inferred from mutant phenotype Ref.4. Source: SGD protein targeting to vacuole Inferred from mutant phenotype PubMed 17895371. Source: SGD
Cellular_component	AP-3 adaptor complex Inferred from mutant phenotype Ref.1. Source: SGD Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasmic vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	protein transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 194	194	AP-3 complex subunit sigma		PRO_0000193822

Sequences

	Sequence		Length	Mass (Da)
	P47064 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 688D81AEA77B7555	FASTA	194	21,938
	10 20 30 40 50 60 MIHAVLIFNK KCQPRLVKFY TPVDLPKQKL LLEQVYELIS QRNSDFQSSF LVTPPSLLLS 70 80 90 100 110 120 NENNNDEVNN EDIQIIYKNY ATLYFTFIVD DQESELAILD LIQTFVESLD RCFTEVNELD 130 140 150 160 170 180 LIFNWQTLES VLEEIVQGGM VIETNVNRIV ASVDELNKAA ESTDSKIGRL TSTGFGSALQ 190 AFAQGGFAQW ATGQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Suppressors of YCK-encoded yeast casein kinase 1 deficiency define the four subunits of a novel clathrin AP-like complex." Panek H.R., Stepp J.D., Engle H.M., Marks K.M., Tan P.K., Lemmon S.K., Robinson L.C. EMBO J. 16:4194-4204(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], IDENTIFICATION IN THE AP-3 COMPLEX, FUNCTION OF THE AP-3 COMPLEX.
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"The AP-3 adaptor complex is essential for cargo-selective transport to the yeast vacuole." Cowles C.R., Odorizzi G., Payne G.S., Emr S.D. Cell 91:109-118(1997) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE AP-3 COMPLEX, FUNCTION OF THE AP-3 COMPLEX.
[5]	"Formation of AP-3 transport intermediates requires Vps41 function." Rehling P., Darsow T., Katzmann D.J., Emr S.D. Nat. Cell Biol. 1:346-353(1999) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, FUNCTION OF THE AP-3 COMPLEX.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U31448 Genomic DNA. Translation: AAA92051.1. Z49299 Genomic DNA. Translation: CAA89315.1. BK006943 Genomic DNA. Translation: DAA08774.1.
PIR	S56796.
RefSeq	NP_012510.3. NM_001181458.3.
3D structure databases
ProteinModelPortal	P47064.
SMR	P47064. Positions 1-150.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3978N.
IntAct	P47064. 2 interactions.
MINT	MINT-565998.
STRING	4932.YJL024C.
Proteomic databases
PaxDb	P47064.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJL024C; YJL024C; YJL024C.
GeneID	853429.
KEGG	sce:YJL019W. sce:YJL024C.
Organism-specific databases
CYGD	YJL024c.
SGD	S000003561. APS3.
Phylogenomic databases
eggNOG	COG5030.
GeneTree	ENSGT00550000074761.
HOGENOM	HOG000185228.
OMA	IFHADAV.
OrthoDB	EOG4M3DJV.
Gene expression databases
Genevestigator	P47064.
GermOnline	YJL024C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR016635. AP_complex_ssu. IPR022775. AP_mu_sigma_su. IPR027155. APS3. IPR000804. Clathrin_sm-chain_CS. IPR011012. Longin-like_dom. [Graphical view]
PANTHER	PTHR11753. PTHR11753. 1 hit. PTHR11753:SF2. PTHR11753:SF2. 1 hit.
Pfam	PF01217. Clat_adaptor_s. 1 hit. [Graphical view]
PIRSF	PIRSF015588. AP_complex_sigma. 1 hit.
SUPFAM	SSF64356. Longin_like. 1 hit.
PROSITE	PS00989. CLAT_ADAPTOR_S. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	973968.

Entry information

Entry name

AP3S_YEAST

Accession

Primary (citable) accession number: P47064
Secondary accession number(s): D6VWF8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program