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Protein

AP-3 complex subunit sigma

Gene

APS3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to the vacuole. Required for the transport via the ALP pathway, which directs the transport of the cargo proteins PHO8 and VAM3 to the vacuole.3 Publications

GO - Molecular functioni

GO - Biological processi

  • Golgi to vacuole transport Source: SGD
  • protein targeting to vacuole Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31494-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
AP-3 complex subunit sigma
Alternative name(s):
AP-3 complex sigma3A subunit
Adaptor-related protein complex 3 subunit sigma
Clathrin-associated/assembly/adaptor protein, small 3
Sigma-adaptin 3A
Sigma3-adaptin
Gene namesi
Name:APS3
Synonyms:YKS7
Ordered Locus Names:YJL024C
ORF Names:J1274
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome X

Organism-specific databases

CYGDiYJL024c.
EuPathDBiFungiDB:YJL024C.
SGDiS000003561. APS3.

Subcellular locationi

GO - Cellular componenti

  • AP-3 adaptor complex Source: SGD
  • cytoplasmic vesicle membrane Source: UniProtKB-SubCell
  • Golgi apparatus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 194194AP-3 complex subunit sigmaPRO_0000193822Add
BLAST

Proteomic databases

MaxQBiP47064.
PaxDbiP47064.

Expressioni

Gene expression databases

GenevestigatoriP47064.

Interactioni

Subunit structurei

Adaptor protein complex 3 (AP-3) is a heterotetramer composed of 2 large adaptins (APL5 and APL6), a medium adaptin (APM3) and a small adaptin (APS3).2 Publications

Protein-protein interaction databases

BioGridi33735. 70 interactions.
DIPiDIP-3978N.
IntActiP47064. 3 interactions.
MINTiMINT-565998.
STRINGi4932.YJL024C.

Structurei

3D structure databases

ProteinModelPortaliP47064.
SMRiP47064. Positions 1-150.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG5030.
GeneTreeiENSGT00550000074761.
HOGENOMiHOG000185228.
InParanoidiP47064.
KOiK12399.
OMAiLSEMIIG.
OrthoDBiEOG79KPTF.

Family and domain databases

InterProiIPR016635. AP_complex_ssu.
IPR022775. AP_mu_sigma_su.
IPR027155. APS3.
IPR000804. Clathrin_sm-chain_CS.
IPR011012. Longin-like_dom.
[Graphical view]
PANTHERiPTHR11753. PTHR11753. 1 hit.
PTHR11753:SF2. PTHR11753:SF2. 1 hit.
PfamiPF01217. Clat_adaptor_s. 1 hit.
[Graphical view]
PIRSFiPIRSF015588. AP_complex_sigma. 1 hit.
SUPFAMiSSF64356. SSF64356. 1 hit.
PROSITEiPS00989. CLAT_ADAPTOR_S. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47064-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIHAVLIFNK KCQPRLVKFY TPVDLPKQKL LLEQVYELIS QRNSDFQSSF
60 70 80 90 100
LVTPPSLLLS NENNNDEVNN EDIQIIYKNY ATLYFTFIVD DQESELAILD
110 120 130 140 150
LIQTFVESLD RCFTEVNELD LIFNWQTLES VLEEIVQGGM VIETNVNRIV
160 170 180 190
ASVDELNKAA ESTDSKIGRL TSTGFGSALQ AFAQGGFAQW ATGQ
Length:194
Mass (Da):21,938
Last modified:November 1, 1995 - v1
Checksum:i688D81AEA77B7555
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U31448 Genomic DNA. Translation: AAA92051.1.
Z49299 Genomic DNA. Translation: CAA89315.1.
BK006943 Genomic DNA. Translation: DAA08774.1.
PIRiS56796.
RefSeqiNP_012510.3. NM_001181458.3.

Genome annotation databases

EnsemblFungiiYJL024C; YJL024C; YJL024C.
GeneIDi853429.
KEGGisce:YJL024C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U31448 Genomic DNA. Translation: AAA92051.1.
Z49299 Genomic DNA. Translation: CAA89315.1.
BK006943 Genomic DNA. Translation: DAA08774.1.
PIRiS56796.
RefSeqiNP_012510.3. NM_001181458.3.

3D structure databases

ProteinModelPortaliP47064.
SMRiP47064. Positions 1-150.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33735. 70 interactions.
DIPiDIP-3978N.
IntActiP47064. 3 interactions.
MINTiMINT-565998.
STRINGi4932.YJL024C.

Proteomic databases

MaxQBiP47064.
PaxDbiP47064.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJL024C; YJL024C; YJL024C.
GeneIDi853429.
KEGGisce:YJL024C.

Organism-specific databases

CYGDiYJL024c.
EuPathDBiFungiDB:YJL024C.
SGDiS000003561. APS3.

Phylogenomic databases

eggNOGiCOG5030.
GeneTreeiENSGT00550000074761.
HOGENOMiHOG000185228.
InParanoidiP47064.
KOiK12399.
OMAiLSEMIIG.
OrthoDBiEOG79KPTF.

Enzyme and pathway databases

BioCyciYEAST:G3O-31494-MONOMER.

Miscellaneous databases

NextBioi973968.
PROiP47064.

Gene expression databases

GenevestigatoriP47064.

Family and domain databases

InterProiIPR016635. AP_complex_ssu.
IPR022775. AP_mu_sigma_su.
IPR027155. APS3.
IPR000804. Clathrin_sm-chain_CS.
IPR011012. Longin-like_dom.
[Graphical view]
PANTHERiPTHR11753. PTHR11753. 1 hit.
PTHR11753:SF2. PTHR11753:SF2. 1 hit.
PfamiPF01217. Clat_adaptor_s. 1 hit.
[Graphical view]
PIRSFiPIRSF015588. AP_complex_sigma. 1 hit.
SUPFAMiSSF64356. SSF64356. 1 hit.
PROSITEiPS00989. CLAT_ADAPTOR_S. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Suppressors of YCK-encoded yeast casein kinase 1 deficiency define the four subunits of a novel clathrin AP-like complex."
    Panek H.R., Stepp J.D., Engle H.M., Marks K.M., Tan P.K., Lemmon S.K., Robinson L.C.
    EMBO J. 16:4194-4204(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], IDENTIFICATION IN THE AP-3 COMPLEX, FUNCTION OF THE AP-3 COMPLEX.
  2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The AP-3 adaptor complex is essential for cargo-selective transport to the yeast vacuole."
    Cowles C.R., Odorizzi G., Payne G.S., Emr S.D.
    Cell 91:109-118(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE AP-3 COMPLEX, FUNCTION OF THE AP-3 COMPLEX.
  5. "Formation of AP-3 transport intermediates requires Vps41 function."
    Rehling P., Darsow T., Katzmann D.J., Emr S.D.
    Nat. Cell Biol. 1:346-353(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, FUNCTION OF THE AP-3 COMPLEX.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiAP3S_YEAST
AccessioniPrimary (citable) accession number: P47064
Secondary accession number(s): D6VWF8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 27, 2015
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1240 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.