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P47061 (VPS53_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Vacuolar protein sorting-associated protein 53
Gene names
Name:VPS53
Ordered Locus Names:YJL029C
ORF Names:J1258
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length822 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in retrograde transport from early and late endosomes to late Golgi by linking the vesicle through the t-SNARE TGL1 to the Golgi, leading to the membrane fusion between late Golgi and endosomal vesicles. Ref.3 Ref.4 Ref.5 Ref.7 Ref.8

Subunit structure

Component of the Golgi-associated retrograde protein (GARP) complex, also called VFT (VPS fifty-three) complex, composed of VPS51, VPS52, VPS53 and VPS54. Interacts also with TLG1, YPT6 and ARL1. Ref.3 Ref.4 Ref.6 Ref.7 Ref.8

Subcellular location

Golgi apparatustrans-Golgi network membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein Ref.5 Ref.9.

Sequence similarities

Belongs to the VPS53 family.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 822822Vacuolar protein sorting-associated protein 53
PRO_0000215193

Regions

Coiled coil34 – 5825 Potential
Coiled coil460 – 48425 Potential

Secondary structure

........................ 822
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P47061 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: C75F19FA57157481

FASTA82295,449
        10         20         30         40         50         60 
MLEGTVDYDP LEDITNILFS KESLNNIDEL ISITRSYKKQ LQEDILKEEN ELKEHPKNSA 

        70         80         90        100        110        120 
EIEASLRKVF QDFKETQDVS ASTELTISNL TEGISYLDIA KKNLTHSLTL FQNLKILTDS 

       130        140        150        160        170        180 
YIQCNELLSQ GSFKKMVSPY KIMCSLAENT FISYKSLDEI NYLLSSISRL KGDTLSKIKQ 

       190        200        210        220        230        240 
NYNALFSGGN ISEHDTALTM ELREGACELL DCDTSTRAQM IDWCLDKLLF EMKEIFRVDD 

       250        260        270        280        290        300 
EAGSLENLSR RYIYFKKILN NFNSKFADYF LKDWEMAVRL TTTFYHITHK DLQTLLKREF 

       310        320        330        340        350        360 
KDKNPSIDLF MTALQSTLDF EKYIDVRFSK KIKEPKLSSC FEPYLTLWVS HQNQMMEKKF 

       370        380        390        400        410        420 
LSYMSEPKYP SNETESLVLP SSADLFRTYR SVLTQTLELI DNNANDSILT SLANFFSRWL 

       430        440        450        460        470        480 
QTYSQKILLP LLLPDNIEVQ DKLEAAKYTV LLINTADYCA TTIDQLEDKL SEFSGNREKL 

       490        500        510        520        530        540 
ANSFTKTKNI YDDLLAKGTS FLLNRVIPLD LNFVWREFIN NDWSNAAIED YSRYMVTLKS 

       550        560        570        580        590        600 
VLKMPALTDA SIKQQQEQPS TLAFILSQFN RDVYKWNFLD KVIDIITTNF VSNTIRLLQP 

       610        620        630        640        650        660 
VPPFSLAGSK RKFETRTVVN IGEQLLLDLE LLKEIFHTLP ESVSNDSDLR ENTSYKRVKR 

       670        680        690        700        710        720 
HADNNIDQLL KFIKLLMAPL DSADDYYETY SKLTNNNPDS AVWSFVLALK GIPWDLALWK 

       730        740        750        760        770        780 
KLWSAYNLET DDTDEGSRPD SNRDLFIFKW DKVLLGQFEN NLARMQDPNW SKFVRQDLKI 

       790        800        810        820 
SPPVMKRIVS TPQIQQQKEE QKKQSLSVKD FVSHSRFFNR GT 

« Hide

References

« Hide 'large scale' references
[1]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Vps52p, Vps53p, and Vps54p form a novel multisubunit complex required for protein sorting at the yeast late Golgi."
Conibear E., Stevens T.H.
Mol. Biol. Cell 11:305-323(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH VPS52 AND VPS54.
[4]"An effector of Ypt6p binds the SNARE Tlg1p and mediates selective fusion of vesicles with late Golgi membranes."
Siniossoglou S., Pelham H.R.B.
EMBO J. 20:5991-5998(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH TLG1 AND YPT6.
[5]"Vps51p links the VFT complex to the SNARE Tlg1p."
Siniossoglou S., Pelham H.R.B.
J. Biol. Chem. 277:48318-48324(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]"The ARF-like GTPases Arl1p and Arl3p act in a pathway that interacts with vesicle-tethering factors at the Golgi apparatus."
Panic B., Whyte J.R.C., Munro S.
Curr. Biol. 13:405-410(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ARL1.
[7]"Vps51 is part of the yeast Vps fifty-three tethering complex essential for retrograde traffic from the early endosome and Cvt vesicle completion."
Reggiori F., Wang C.-W., Stromhaug P.E., Shintani T., Klionsky D.J.
J. Biol. Chem. 278:5009-5020(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH VPS51; VPS52 AND VPS54.
[8]"Vps51p mediates the association of the GARP (Vps52/53/54) complex with the late Golgi t-SNARE Tlg1p."
Conibear E., Cleck J.N., Stevens T.H.
Mol. Biol. Cell 14:1610-1623(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH VPS51; VPS52; VPS54 AND TLG1.
[9]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[10]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z49304 Genomic DNA. Translation: CAA89320.1.
BK006943 Genomic DNA. Translation: DAA08769.1.
PIRS56801.
RefSeqNP_012505.1. NM_001181463.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3NS4X-ray2.90A554-822[»]
ProteinModelPortalP47061.
SMRP47061. Positions 564-779.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33730. 81 interactions.
DIPDIP-5902N.
IntActP47061. 8 interactions.
MINTMINT-619612.
STRING4932.YJL029C.

Proteomic databases

MaxQBP47061.
PaxDbP47061.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJL029C; YJL029C; YJL029C.
GeneID853423.
KEGGsce:YJL029C.

Organism-specific databases

CYGDYJL029c.
SGDS000003566. VPS53.

Phylogenomic databases

eggNOGNOG327715.
GeneTreeENSGT00390000015165.
HOGENOMHOG000000812.
OMAVEDYSRY.
OrthoDBEOG7NKKTQ.

Enzyme and pathway databases

BioCycYEAST:G3O-31498-MONOMER.

Gene expression databases

GenevestigatorP47061.

Family and domain databases

InterProIPR007234. Vps53_N.
[Graphical view]
PfamPF04100. Vps53_N. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP47061.
NextBio973951.
PROP47061.

Entry information

Entry nameVPS53_YEAST
AccessionPrimary (citable) accession number: P47061
Secondary accession number(s): D6VWF3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: June 11, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references