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P47061

- VPS53_YEAST

UniProt

P47061 - VPS53_YEAST

Protein

Vacuolar protein sorting-associated protein 53

Gene

VPS53

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Involved in retrograde transport from early and late endosomes to late Golgi by linking the vesicle through the t-SNARE TGL1 to the Golgi, leading to the membrane fusion between late Golgi and endosomal vesicles.5 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. Golgi to vacuole transport Source: SGD
    2. protein transport Source: UniProtKB-KW
    3. retrograde transport, endosome to Golgi Source: SGD

    Keywords - Biological processi

    Protein transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31498-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Vacuolar protein sorting-associated protein 53
    Gene namesi
    Name:VPS53
    Ordered Locus Names:YJL029C
    ORF Names:J1258
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome X

    Organism-specific databases

    CYGDiYJL029c.
    SGDiS000003566. VPS53.

    Subcellular locationi

    GO - Cellular componenti

    1. endosome membrane Source: UniProtKB-SubCell
    2. GARP complex Source: SGD
    3. Golgi apparatus Source: SGD

    Keywords - Cellular componenti

    Endosome, Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 822822Vacuolar protein sorting-associated protein 53PRO_0000215193Add
    BLAST

    Proteomic databases

    MaxQBiP47061.
    PaxDbiP47061.

    Expressioni

    Gene expression databases

    GenevestigatoriP47061.

    Interactioni

    Subunit structurei

    Component of the Golgi-associated retrograde protein (GARP) complex, also called VFT (VPS fifty-three) complex, composed of VPS51, VPS52, VPS53 and VPS54. Interacts also with TLG1, YPT6 and ARL1.5 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ARL1P381163EBI-25828,EBI-2869
    VPS52P399048EBI-25828,EBI-16418
    VPS54Q1207110EBI-25828,EBI-36751

    Protein-protein interaction databases

    BioGridi33730. 81 interactions.
    DIPiDIP-5902N.
    IntActiP47061. 8 interactions.
    MINTiMINT-619612.
    STRINGi4932.YJL029C.

    Structurei

    Secondary structure

    1
    822
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi567 – 5693
    Helixi571 – 59828
    Beta strandi605 – 6084
    Helixi615 – 63824
    Helixi639 – 6435
    Helixi653 – 67624
    Helixi683 – 69311
    Helixi700 – 71011
    Helixi716 – 72914
    Helixi746 – 7505
    Helixi752 – 76312
    Helixi768 – 7769

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3NS4X-ray2.90A554-822[»]
    ProteinModelPortaliP47061.
    SMRiP47061. Positions 564-779.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP47061.

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili34 – 5825Sequence AnalysisAdd
    BLAST
    Coiled coili460 – 48425Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the VPS53 family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG327715.
    GeneTreeiENSGT00390000015165.
    HOGENOMiHOG000000812.
    OMAiVEDYSRY.
    OrthoDBiEOG7NKKTQ.

    Family and domain databases

    InterProiIPR007234. Vps53_N.
    [Graphical view]
    PfamiPF04100. Vps53_N. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P47061-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLEGTVDYDP LEDITNILFS KESLNNIDEL ISITRSYKKQ LQEDILKEEN    50
    ELKEHPKNSA EIEASLRKVF QDFKETQDVS ASTELTISNL TEGISYLDIA 100
    KKNLTHSLTL FQNLKILTDS YIQCNELLSQ GSFKKMVSPY KIMCSLAENT 150
    FISYKSLDEI NYLLSSISRL KGDTLSKIKQ NYNALFSGGN ISEHDTALTM 200
    ELREGACELL DCDTSTRAQM IDWCLDKLLF EMKEIFRVDD EAGSLENLSR 250
    RYIYFKKILN NFNSKFADYF LKDWEMAVRL TTTFYHITHK DLQTLLKREF 300
    KDKNPSIDLF MTALQSTLDF EKYIDVRFSK KIKEPKLSSC FEPYLTLWVS 350
    HQNQMMEKKF LSYMSEPKYP SNETESLVLP SSADLFRTYR SVLTQTLELI 400
    DNNANDSILT SLANFFSRWL QTYSQKILLP LLLPDNIEVQ DKLEAAKYTV 450
    LLINTADYCA TTIDQLEDKL SEFSGNREKL ANSFTKTKNI YDDLLAKGTS 500
    FLLNRVIPLD LNFVWREFIN NDWSNAAIED YSRYMVTLKS VLKMPALTDA 550
    SIKQQQEQPS TLAFILSQFN RDVYKWNFLD KVIDIITTNF VSNTIRLLQP 600
    VPPFSLAGSK RKFETRTVVN IGEQLLLDLE LLKEIFHTLP ESVSNDSDLR 650
    ENTSYKRVKR HADNNIDQLL KFIKLLMAPL DSADDYYETY SKLTNNNPDS 700
    AVWSFVLALK GIPWDLALWK KLWSAYNLET DDTDEGSRPD SNRDLFIFKW 750
    DKVLLGQFEN NLARMQDPNW SKFVRQDLKI SPPVMKRIVS TPQIQQQKEE 800
    QKKQSLSVKD FVSHSRFFNR GT 822
    Length:822
    Mass (Da):95,449
    Last modified:February 1, 1996 - v1
    Checksum:iC75F19FA57157481
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49304 Genomic DNA. Translation: CAA89320.1.
    BK006943 Genomic DNA. Translation: DAA08769.1.
    PIRiS56801.
    RefSeqiNP_012505.1. NM_001181463.1.

    Genome annotation databases

    EnsemblFungiiYJL029C; YJL029C; YJL029C.
    GeneIDi853423.
    KEGGisce:YJL029C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49304 Genomic DNA. Translation: CAA89320.1 .
    BK006943 Genomic DNA. Translation: DAA08769.1 .
    PIRi S56801.
    RefSeqi NP_012505.1. NM_001181463.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3NS4 X-ray 2.90 A 554-822 [» ]
    ProteinModelPortali P47061.
    SMRi P47061. Positions 564-779.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33730. 81 interactions.
    DIPi DIP-5902N.
    IntActi P47061. 8 interactions.
    MINTi MINT-619612.
    STRINGi 4932.YJL029C.

    Proteomic databases

    MaxQBi P47061.
    PaxDbi P47061.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YJL029C ; YJL029C ; YJL029C .
    GeneIDi 853423.
    KEGGi sce:YJL029C.

    Organism-specific databases

    CYGDi YJL029c.
    SGDi S000003566. VPS53.

    Phylogenomic databases

    eggNOGi NOG327715.
    GeneTreei ENSGT00390000015165.
    HOGENOMi HOG000000812.
    OMAi VEDYSRY.
    OrthoDBi EOG7NKKTQ.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31498-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P47061.
    NextBioi 973951.
    PROi P47061.

    Gene expression databases

    Genevestigatori P47061.

    Family and domain databases

    InterProi IPR007234. Vps53_N.
    [Graphical view ]
    Pfami PF04100. Vps53_N. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Vps52p, Vps53p, and Vps54p form a novel multisubunit complex required for protein sorting at the yeast late Golgi."
      Conibear E., Stevens T.H.
      Mol. Biol. Cell 11:305-323(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH VPS52 AND VPS54.
    4. "An effector of Ypt6p binds the SNARE Tlg1p and mediates selective fusion of vesicles with late Golgi membranes."
      Siniossoglou S., Pelham H.R.B.
      EMBO J. 20:5991-5998(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH TLG1 AND YPT6.
    5. "Vps51p links the VFT complex to the SNARE Tlg1p."
      Siniossoglou S., Pelham H.R.B.
      J. Biol. Chem. 277:48318-48324(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    6. "The ARF-like GTPases Arl1p and Arl3p act in a pathway that interacts with vesicle-tethering factors at the Golgi apparatus."
      Panic B., Whyte J.R.C., Munro S.
      Curr. Biol. 13:405-410(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ARL1.
    7. "Vps51 is part of the yeast Vps fifty-three tethering complex essential for retrograde traffic from the early endosome and Cvt vesicle completion."
      Reggiori F., Wang C.-W., Stromhaug P.E., Shintani T., Klionsky D.J.
      J. Biol. Chem. 278:5009-5020(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH VPS51; VPS52 AND VPS54.
    8. "Vps51p mediates the association of the GARP (Vps52/53/54) complex with the late Golgi t-SNARE Tlg1p."
      Conibear E., Cleck J.N., Stevens T.H.
      Mol. Biol. Cell 14:1610-1623(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH VPS51; VPS52; VPS54 AND TLG1.
    9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    10. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiVPS53_YEAST
    AccessioniPrimary (citable) accession number: P47061
    Secondary accession number(s): D6VWF3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3