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P47061

- VPS53_YEAST

UniProt

P47061 - VPS53_YEAST

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Protein
Vacuolar protein sorting-associated protein 53
Gene
VPS53, YJL029C, J1258
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in retrograde transport from early and late endosomes to late Golgi by linking the vesicle through the t-SNARE TGL1 to the Golgi, leading to the membrane fusion between late Golgi and endosomal vesicles.5 Publications

GO - Molecular functioni

  1. protein binding Source: IntAct
Complete GO annotation...

GO - Biological processi

  1. Golgi to vacuole transport Source: SGD
  2. protein transport Source: UniProtKB-KW
  3. retrograde transport, endosome to Golgi Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31498-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein sorting-associated protein 53
Gene namesi
Name:VPS53
Ordered Locus Names:YJL029C
ORF Names:J1258
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

CYGDiYJL029c.
SGDiS000003566. VPS53.

Subcellular locationi

Golgi apparatustrans-Golgi network membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein 2 Publications

GO - Cellular componenti

  1. GARP complex Source: SGD
  2. Golgi apparatus Source: SGD
  3. endosome membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 822822Vacuolar protein sorting-associated protein 53
PRO_0000215193Add
BLAST

Proteomic databases

MaxQBiP47061.
PaxDbiP47061.

Expressioni

Gene expression databases

GenevestigatoriP47061.

Interactioni

Subunit structurei

Component of the Golgi-associated retrograde protein (GARP) complex, also called VFT (VPS fifty-three) complex, composed of VPS51, VPS52, VPS53 and VPS54. Interacts also with TLG1, YPT6 and ARL1.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ARL1P381163EBI-25828,EBI-2869
VPS52P399048EBI-25828,EBI-16418
VPS54Q1207110EBI-25828,EBI-36751

Protein-protein interaction databases

BioGridi33730. 81 interactions.
DIPiDIP-5902N.
IntActiP47061. 8 interactions.
MINTiMINT-619612.
STRINGi4932.YJL029C.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi567 – 5693
Helixi571 – 59828
Beta strandi605 – 6084
Helixi615 – 63824
Helixi639 – 6435
Helixi653 – 67624
Helixi683 – 69311
Helixi700 – 71011
Helixi716 – 72914
Helixi746 – 7505
Helixi752 – 76312
Helixi768 – 7769

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3NS4X-ray2.90A554-822[»]
ProteinModelPortaliP47061.
SMRiP47061. Positions 564-779.

Miscellaneous databases

EvolutionaryTraceiP47061.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili34 – 5825 Reviewed prediction
Add
BLAST
Coiled coili460 – 48425 Reviewed prediction
Add
BLAST

Sequence similaritiesi

Belongs to the VPS53 family.

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG327715.
GeneTreeiENSGT00390000015165.
HOGENOMiHOG000000812.
OMAiVEDYSRY.
OrthoDBiEOG7NKKTQ.

Family and domain databases

InterProiIPR007234. Vps53_N.
[Graphical view]
PfamiPF04100. Vps53_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47061-1 [UniParc]FASTAAdd to Basket

« Hide

MLEGTVDYDP LEDITNILFS KESLNNIDEL ISITRSYKKQ LQEDILKEEN    50
ELKEHPKNSA EIEASLRKVF QDFKETQDVS ASTELTISNL TEGISYLDIA 100
KKNLTHSLTL FQNLKILTDS YIQCNELLSQ GSFKKMVSPY KIMCSLAENT 150
FISYKSLDEI NYLLSSISRL KGDTLSKIKQ NYNALFSGGN ISEHDTALTM 200
ELREGACELL DCDTSTRAQM IDWCLDKLLF EMKEIFRVDD EAGSLENLSR 250
RYIYFKKILN NFNSKFADYF LKDWEMAVRL TTTFYHITHK DLQTLLKREF 300
KDKNPSIDLF MTALQSTLDF EKYIDVRFSK KIKEPKLSSC FEPYLTLWVS 350
HQNQMMEKKF LSYMSEPKYP SNETESLVLP SSADLFRTYR SVLTQTLELI 400
DNNANDSILT SLANFFSRWL QTYSQKILLP LLLPDNIEVQ DKLEAAKYTV 450
LLINTADYCA TTIDQLEDKL SEFSGNREKL ANSFTKTKNI YDDLLAKGTS 500
FLLNRVIPLD LNFVWREFIN NDWSNAAIED YSRYMVTLKS VLKMPALTDA 550
SIKQQQEQPS TLAFILSQFN RDVYKWNFLD KVIDIITTNF VSNTIRLLQP 600
VPPFSLAGSK RKFETRTVVN IGEQLLLDLE LLKEIFHTLP ESVSNDSDLR 650
ENTSYKRVKR HADNNIDQLL KFIKLLMAPL DSADDYYETY SKLTNNNPDS 700
AVWSFVLALK GIPWDLALWK KLWSAYNLET DDTDEGSRPD SNRDLFIFKW 750
DKVLLGQFEN NLARMQDPNW SKFVRQDLKI SPPVMKRIVS TPQIQQQKEE 800
QKKQSLSVKD FVSHSRFFNR GT 822
Length:822
Mass (Da):95,449
Last modified:February 1, 1996 - v1
Checksum:iC75F19FA57157481
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z49304 Genomic DNA. Translation: CAA89320.1.
BK006943 Genomic DNA. Translation: DAA08769.1.
PIRiS56801.
RefSeqiNP_012505.1. NM_001181463.1.

Genome annotation databases

EnsemblFungiiYJL029C; YJL029C; YJL029C.
GeneIDi853423.
KEGGisce:YJL029C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z49304 Genomic DNA. Translation: CAA89320.1 .
BK006943 Genomic DNA. Translation: DAA08769.1 .
PIRi S56801.
RefSeqi NP_012505.1. NM_001181463.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3NS4 X-ray 2.90 A 554-822 [» ]
ProteinModelPortali P47061.
SMRi P47061. Positions 564-779.
ModBasei Search...

Protein-protein interaction databases

BioGridi 33730. 81 interactions.
DIPi DIP-5902N.
IntActi P47061. 8 interactions.
MINTi MINT-619612.
STRINGi 4932.YJL029C.

Proteomic databases

MaxQBi P47061.
PaxDbi P47061.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YJL029C ; YJL029C ; YJL029C .
GeneIDi 853423.
KEGGi sce:YJL029C.

Organism-specific databases

CYGDi YJL029c.
SGDi S000003566. VPS53.

Phylogenomic databases

eggNOGi NOG327715.
GeneTreei ENSGT00390000015165.
HOGENOMi HOG000000812.
OMAi VEDYSRY.
OrthoDBi EOG7NKKTQ.

Enzyme and pathway databases

BioCyci YEAST:G3O-31498-MONOMER.

Miscellaneous databases

EvolutionaryTracei P47061.
NextBioi 973951.
PROi P47061.

Gene expression databases

Genevestigatori P47061.

Family and domain databases

InterProi IPR007234. Vps53_N.
[Graphical view ]
Pfami PF04100. Vps53_N. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Vps52p, Vps53p, and Vps54p form a novel multisubunit complex required for protein sorting at the yeast late Golgi."
    Conibear E., Stevens T.H.
    Mol. Biol. Cell 11:305-323(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH VPS52 AND VPS54.
  4. "An effector of Ypt6p binds the SNARE Tlg1p and mediates selective fusion of vesicles with late Golgi membranes."
    Siniossoglou S., Pelham H.R.B.
    EMBO J. 20:5991-5998(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH TLG1 AND YPT6.
  5. "Vps51p links the VFT complex to the SNARE Tlg1p."
    Siniossoglou S., Pelham H.R.B.
    J. Biol. Chem. 277:48318-48324(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  6. "The ARF-like GTPases Arl1p and Arl3p act in a pathway that interacts with vesicle-tethering factors at the Golgi apparatus."
    Panic B., Whyte J.R.C., Munro S.
    Curr. Biol. 13:405-410(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ARL1.
  7. "Vps51 is part of the yeast Vps fifty-three tethering complex essential for retrograde traffic from the early endosome and Cvt vesicle completion."
    Reggiori F., Wang C.-W., Stromhaug P.E., Shintani T., Klionsky D.J.
    J. Biol. Chem. 278:5009-5020(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH VPS51; VPS52 AND VPS54.
  8. "Vps51p mediates the association of the GARP (Vps52/53/54) complex with the late Golgi t-SNARE Tlg1p."
    Conibear E., Cleck J.N., Stevens T.H.
    Mol. Biol. Cell 14:1610-1623(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH VPS51; VPS52; VPS54 AND TLG1.
  9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  10. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiVPS53_YEAST
AccessioniPrimary (citable) accession number: P47061
Secondary accession number(s): D6VWF3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: June 11, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3

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