Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Vacuolar protein sorting-associated protein 53

Gene

VPS53

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in retrograde transport from early and late endosomes to late Golgi by linking the vesicle through the t-SNARE TGL1 to the Golgi, leading to the membrane fusion between late Golgi and endosomal vesicles.5 Publications

GO - Biological processi

  • cellular sphingolipid homeostasis Source: SGD
  • Golgi to vacuole transport Source: SGD
  • protein transport Source: UniProtKB-KW
  • retrograde transport, endosome to Golgi Source: SGD

Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31498-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein sorting-associated protein 53
Gene namesi
Name:VPS53
Ordered Locus Names:YJL029C
ORF Names:J1258
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJL029C
SGDiS000003566 VPS53

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endosome, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002151931 – 822Vacuolar protein sorting-associated protein 53Add BLAST822

Proteomic databases

MaxQBiP47061
PaxDbiP47061
PRIDEiP47061

PTM databases

iPTMnetiP47061

Interactioni

Subunit structurei

Component of the Golgi-associated retrograde protein (GARP) complex, also called VFT (VPS fifty-three) complex, composed of VPS51, VPS52, VPS53 and VPS54. Interacts also with TLG1, YPT6 and ARL1.5 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi33730, 706 interactors
DIPiDIP-5902N
IntActiP47061, 8 interactors
MINTiP47061
STRINGi4932.YJL029C

Structurei

Secondary structure

1822
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi567 – 569Combined sources3
Helixi571 – 598Combined sources28
Beta strandi605 – 608Combined sources4
Helixi615 – 638Combined sources24
Helixi639 – 643Combined sources5
Helixi653 – 676Combined sources24
Helixi683 – 693Combined sources11
Helixi700 – 710Combined sources11
Helixi716 – 729Combined sources14
Helixi746 – 750Combined sources5
Helixi752 – 763Combined sources12
Helixi768 – 776Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3NS4X-ray2.90A554-822[»]
ProteinModelPortaliP47061
SMRiP47061
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP47061

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili34 – 58Sequence analysisAdd BLAST25
Coiled coili460 – 484Sequence analysisAdd BLAST25

Sequence similaritiesi

Belongs to the VPS53 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00390000015165
HOGENOMiHOG000000812
InParanoidiP47061
KOiK20299
OMAiNSSEYCH
OrthoDBiEOG092C12WO

Family and domain databases

Gene3Di1.10.357.110, 1 hit
InterProiView protein in InterPro
IPR031745 Vps53_C
IPR038260 Vps53_C_sf
IPR007234 Vps53_N
PfamiView protein in Pfam
PF16854 VPS53_C, 1 hit
PF04100 Vps53_N, 1 hit

Sequencei

Sequence statusi: Complete.

P47061-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLEGTVDYDP LEDITNILFS KESLNNIDEL ISITRSYKKQ LQEDILKEEN
60 70 80 90 100
ELKEHPKNSA EIEASLRKVF QDFKETQDVS ASTELTISNL TEGISYLDIA
110 120 130 140 150
KKNLTHSLTL FQNLKILTDS YIQCNELLSQ GSFKKMVSPY KIMCSLAENT
160 170 180 190 200
FISYKSLDEI NYLLSSISRL KGDTLSKIKQ NYNALFSGGN ISEHDTALTM
210 220 230 240 250
ELREGACELL DCDTSTRAQM IDWCLDKLLF EMKEIFRVDD EAGSLENLSR
260 270 280 290 300
RYIYFKKILN NFNSKFADYF LKDWEMAVRL TTTFYHITHK DLQTLLKREF
310 320 330 340 350
KDKNPSIDLF MTALQSTLDF EKYIDVRFSK KIKEPKLSSC FEPYLTLWVS
360 370 380 390 400
HQNQMMEKKF LSYMSEPKYP SNETESLVLP SSADLFRTYR SVLTQTLELI
410 420 430 440 450
DNNANDSILT SLANFFSRWL QTYSQKILLP LLLPDNIEVQ DKLEAAKYTV
460 470 480 490 500
LLINTADYCA TTIDQLEDKL SEFSGNREKL ANSFTKTKNI YDDLLAKGTS
510 520 530 540 550
FLLNRVIPLD LNFVWREFIN NDWSNAAIED YSRYMVTLKS VLKMPALTDA
560 570 580 590 600
SIKQQQEQPS TLAFILSQFN RDVYKWNFLD KVIDIITTNF VSNTIRLLQP
610 620 630 640 650
VPPFSLAGSK RKFETRTVVN IGEQLLLDLE LLKEIFHTLP ESVSNDSDLR
660 670 680 690 700
ENTSYKRVKR HADNNIDQLL KFIKLLMAPL DSADDYYETY SKLTNNNPDS
710 720 730 740 750
AVWSFVLALK GIPWDLALWK KLWSAYNLET DDTDEGSRPD SNRDLFIFKW
760 770 780 790 800
DKVLLGQFEN NLARMQDPNW SKFVRQDLKI SPPVMKRIVS TPQIQQQKEE
810 820
QKKQSLSVKD FVSHSRFFNR GT
Length:822
Mass (Da):95,449
Last modified:February 1, 1996 - v1
Checksum:iC75F19FA57157481
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49304 Genomic DNA Translation: CAA89320.1
BK006943 Genomic DNA Translation: DAA08769.1
PIRiS56801
RefSeqiNP_012505.1, NM_001181463.1

Genome annotation databases

EnsemblFungiiYJL029C; YJL029C; YJL029C
GeneIDi853423
KEGGisce:YJL029C

Similar proteinsi

Entry informationi

Entry nameiVPS53_YEAST
AccessioniPrimary (citable) accession number: P47061
Secondary accession number(s): D6VWF3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: May 23, 2018
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Protein nomenclature information
    List of publications dealing with protein nomenclature and links to specialized websites

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health