Vacuolar protein sorting-associated protein 53 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Contribute

Send feedback

Read comments (?) or add your own

P47061 (VPS53_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 83. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Vacuolar protein sorting-associated protein 53

Gene names

Name:	VPS53
Ordered Locus Names:	YJL029C
ORF Names:	J1258

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	822 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in retrograde transport from early and late endosomes to late Golgi by linking the vesicle through the t-SNARE TGL1 to the Golgi, leading to the membrane fusion between late Golgi and endosomal vesicles. Ref.3 Ref.4 Ref.5 Ref.7 Ref.8
Subunit structure	Component of the Golgi-associated retrograde protein (GARP) complex, also called VFT (VPS fifty-three) complex, composed of VPS51, VPS52, VPS53 and VPS54. Interacts also with TLG1, YPT6 and ARL1. Ref.3 Ref.4 Ref.6 Ref.7 Ref.8
Subcellular location	Golgi apparatus › trans-Golgi network membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein Ref.5 Ref.9.
Sequence similarities	Belongs to the VPS53 family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Endosome Golgi apparatus Membrane
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	Golgi to vacuole transport Inferred from mutant phenotype Ref.3. Source: SGD protein transport Inferred from electronic annotation. Source: UniProtKB-KW retrograde transport, endosome to Golgi Inferred from direct assay Ref.8. Source: SGD
Cellular component	GARP complex Inferred from physical interaction Ref.3. Source: SGD endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction Ref.6. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
ARL1	P38116	3	EBI-25828,EBI-2869
VPS52	P39904	8	EBI-25828,EBI-16418
VPS54	Q12071	10	EBI-25828,EBI-36751

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 822

822

Vacuolar protein sorting-associated protein 53

PRO_0000215193

Regions

Coiled coil

34 – 58

Potential

Coiled coil

460 – 484

Potential

Amino acid modifications

Modified residue

Phosphoserine Ref.11

Modified residue

790

Phosphoserine Ref.10 Ref.11

Modified residue

791

Phosphothreonine Ref.11

Modified residue

815

Phosphoserine Ref.10

Secondary structure

822

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P47061 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: C75F19FA57157481
Show »

FASTA

822

95,449

        10         20         30         40         50         60 
MLEGTVDYDP LEDITNILFS KESLNNIDEL ISITRSYKKQ LQEDILKEEN ELKEHPKNSA 

        70         80         90        100        110        120 
EIEASLRKVF QDFKETQDVS ASTELTISNL TEGISYLDIA KKNLTHSLTL FQNLKILTDS 

       130        140        150        160        170        180 
YIQCNELLSQ GSFKKMVSPY KIMCSLAENT FISYKSLDEI NYLLSSISRL KGDTLSKIKQ 

       190        200        210        220        230        240 
NYNALFSGGN ISEHDTALTM ELREGACELL DCDTSTRAQM IDWCLDKLLF EMKEIFRVDD 

       250        260        270        280        290        300 
EAGSLENLSR RYIYFKKILN NFNSKFADYF LKDWEMAVRL TTTFYHITHK DLQTLLKREF 

       310        320        330        340        350        360 
KDKNPSIDLF MTALQSTLDF EKYIDVRFSK KIKEPKLSSC FEPYLTLWVS HQNQMMEKKF 

       370        380        390        400        410        420 
LSYMSEPKYP SNETESLVLP SSADLFRTYR SVLTQTLELI DNNANDSILT SLANFFSRWL 

       430        440        450        460        470        480 
QTYSQKILLP LLLPDNIEVQ DKLEAAKYTV LLINTADYCA TTIDQLEDKL SEFSGNREKL 

       490        500        510        520        530        540 
ANSFTKTKNI YDDLLAKGTS FLLNRVIPLD LNFVWREFIN NDWSNAAIED YSRYMVTLKS 

       550        560        570        580        590        600 
VLKMPALTDA SIKQQQEQPS TLAFILSQFN RDVYKWNFLD KVIDIITTNF VSNTIRLLQP 

       610        620        630        640        650        660 
VPPFSLAGSK RKFETRTVVN IGEQLLLDLE LLKEIFHTLP ESVSNDSDLR ENTSYKRVKR 

       670        680        690        700        710        720 
HADNNIDQLL KFIKLLMAPL DSADDYYETY SKLTNNNPDS AVWSFVLALK GIPWDLALWK 

       730        740        750        760        770        780 
KLWSAYNLET DDTDEGSRPD SNRDLFIFKW DKVLLGQFEN NLARMQDPNW SKFVRQDLKI 

       790        800        810        820 
SPPVMKRIVS TPQIQQQKEE QKKQSLSVKD FVSHSRFFNR GT

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Vps52p, Vps53p, and Vps54p form a novel multisubunit complex required for protein sorting at the yeast late Golgi." Conibear E., Stevens T.H. Mol. Biol. Cell 11:305-323(2000) [PubMed: 10637310] [Abstract] Cited for: FUNCTION, INTERACTION WITH VPS52 AND VPS54.
[4]	"An effector of Ypt6p binds the SNARE Tlg1p and mediates selective fusion of vesicles with late Golgi membranes." Siniossoglou S., Pelham H.R.B. EMBO J. 20:5991-5998(2001) [PubMed: 11689439] [Abstract] Cited for: FUNCTION, INTERACTION WITH TLG1 AND YPT6.
[5]	"Vps51p links the VFT complex to the SNARE Tlg1p." Siniossoglou S., Pelham H.R.B. J. Biol. Chem. 277:48318-48324(2002) [PubMed: 12377769] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]	"The ARF-like GTPases Arl1p and Arl3p act in a pathway that interacts with vesicle-tethering factors at the Golgi apparatus." Panic B., Whyte J.R.C., Munro S. Curr. Biol. 13:405-410(2003) [PubMed: 12620189] [Abstract] Cited for: INTERACTION WITH ARL1.
[7]	"Vps51 is part of the yeast Vps fifty-three tethering complex essential for retrograde traffic from the early endosome and Cvt vesicle completion." Reggiori F., Wang C.-W., Stromhaug P.E., Shintani T., Klionsky D.J. J. Biol. Chem. 278:5009-5020(2003) [PubMed: 12446664] [Abstract] Cited for: FUNCTION, INTERACTION WITH VPS51; VPS52 AND VPS54.
[8]	"Vps51p mediates the association of the GARP (Vps52/53/54) complex with the late Golgi t-SNARE Tlg1p." Conibear E., Cleck J.N., Stevens T.H. Mol. Biol. Cell 14:1610-1623(2003) [PubMed: 12686613] [Abstract] Cited for: FUNCTION, INTERACTION WITH VPS51; VPS52; VPS54 AND TLG1.
[9]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[10]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-790 AND SER-815, MASS SPECTROMETRY.
[11]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-32; SER-790 AND THR-791, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z49304 Genomic DNA. Translation: CAA89320.1.
BK006943 Genomic DNA. Translation: DAA08769.1.

PIR

S56801.

RefSeq

NP_012505.1. NM_001181463.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3NS4	X-ray	2.90	A	554-822	[»]

ProteinModelPortal

P47061.

SMR

P47061. Positions 564-779.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-5902N.

IntAct

P47061. 10 interactions.

MINT

MINT-619612.

STRING

P47061.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YJL029C; YJL029C; YJL029C.

GeneID

853423.

KEGG

sce:YJL029C.

NMPDR

fig|4932.3.peg.3479.

Organism-specific databases

CYGD

YJL029c.

SGD

S000003566. VPS53.

Phylogenomic databases

eggNOG

fuNOG06010.

GeneTree

EFGT00050000005533.

HOGENOM

HBG202214.

OMA

VEDYSRY.

OrthoDB

EOG42JS19.

Gene expression databases

ArrayExpress

P47061.

Genevestigator

P47061.

GermOnline

YJL029C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR007234. Vps53_N.
[Graphical view]

Pfam

PF04100. Vps53_N. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

973951.

Entry information

Entry name

VPS53_YEAST

Accession

Primary (citable) accession number: P47061
Secondary accession number(s): D6VWF3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	December 14, 2011
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents