Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

tRNA-specific adenosine deaminase subunit TAD2

Gene

TAD2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Deaminates adenosine-34 to inosine in many tRNAs.

Catalytic activityi

Adenine(34) in tRNA + H2O = hypoxanthine(34) in tRNA + NH3.

Cofactori

Zn2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi54 – 541Zinc; catalyticBy similarity
Active sitei56 – 561Proton donorBy similarity
Metal bindingi88 – 881Zinc; catalyticBy similarity
Metal bindingi91 – 911Zinc; catalyticBy similarity

GO - Molecular functioni

GO - Biological processi

  • tRNA modification Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:YJL035C-MONOMER.
BRENDAi3.5.4.33. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-specific adenosine deaminase subunit TAD2 (EC:3.5.4.33)
Alternative name(s):
tRNA-specific adenosine-34 deaminase subunit TAD2
Gene namesi
Name:TAD2
Ordered Locus Names:YJL035C
ORF Names:J1246
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJL035C.
SGDiS000003572. TAD2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 250250tRNA-specific adenosine deaminase subunit TAD2PRO_0000171740Add
BLAST

Interactioni

Subunit structurei

Heterodimer with TAD3.

Binary interactionsi

WithEntry#Exp.IntActNotes
TAD3Q9URQ32EBI-18939,EBI-2094330

Protein-protein interaction databases

BioGridi33725. 21 interactions.
DIPiDIP-5546N.
IntActiP47058. 1 interaction.
MINTiMINT-488686.

Structurei

3D structure databases

ProteinModelPortaliP47058.
SMRiP47058. Positions 5-157.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 119119CMP/dCMP-type deaminasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 CMP/dCMP-type deaminase domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00390000000280.
HOGENOMiHOG000085051.
InParanoidiP47058.
KOiK15441.
OMAiNERAPKP.
OrthoDBiEOG74FFC7.

Family and domain databases

InterProiIPR016192. APOBEC/CMP_deaminase_Zn-bd.
IPR002125. CMP_dCMP_Zn-bd.
IPR016193. Cytidine_deaminase-like.
[Graphical view]
PfamiPF00383. dCMP_cyt_deam_1. 1 hit.
[Graphical view]
SUPFAMiSSF53927. SSF53927. 1 hit.
PROSITEiPS00903. CYT_DCMP_DEAMINASES_1. 1 hit.
PS51747. CYT_DCMP_DEAMINASES_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47058-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQHIKHMRTA VRLARYALDH DETPVACIFV HTPTGQVMAY GMNDTNKSLT
60 70 80 90 100
GVAHAEFMGI DQIKAMLGSR GVVDVFKDIT LYVTVEPCIM CASALKQLDI
110 120 130 140 150
GKVVFGCGNE RFGGNGTVLS VNHDTCTLVP KNNSAAGYES IPGILRKEAI
160 170 180 190 200
MLLRYFYVRQ NERAPKPRSK SDRVLDKNTF PPMEWSKYLN EEAFIETFGD
210 220 230 240 250
DYRTCFANKV DLSSNSVDWD LIDSHQDNII QELEEQCKMF KFNVHKKSKV
Length:250
Mass (Da):28,252
Last modified:February 1, 1996 - v1
Checksum:iC774A1E50AA68106
GO

Sequence cautioni

The sequence CAA88261.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ242667 Genomic DNA. Translation: CAB60629.1.
Z49310 Genomic DNA. Translation: CAA89326.1.
AY693172 Genomic DNA. Translation: AAT93191.1.
Z48229 Genomic DNA. Translation: CAA88261.1. Different initiation.
BK006943 Genomic DNA. Translation: DAA08764.1.
PIRiS56807.
RefSeqiNP_012499.1. NM_001181469.1.

Genome annotation databases

EnsemblFungiiYJL035C; YJL035C; YJL035C.
GeneIDi853417.
KEGGisce:YJL035C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ242667 Genomic DNA. Translation: CAB60629.1.
Z49310 Genomic DNA. Translation: CAA89326.1.
AY693172 Genomic DNA. Translation: AAT93191.1.
Z48229 Genomic DNA. Translation: CAA88261.1. Different initiation.
BK006943 Genomic DNA. Translation: DAA08764.1.
PIRiS56807.
RefSeqiNP_012499.1. NM_001181469.1.

3D structure databases

ProteinModelPortaliP47058.
SMRiP47058. Positions 5-157.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33725. 21 interactions.
DIPiDIP-5546N.
IntActiP47058. 1 interaction.
MINTiMINT-488686.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJL035C; YJL035C; YJL035C.
GeneIDi853417.
KEGGisce:YJL035C.

Organism-specific databases

EuPathDBiFungiDB:YJL035C.
SGDiS000003572. TAD2.

Phylogenomic databases

GeneTreeiENSGT00390000000280.
HOGENOMiHOG000085051.
InParanoidiP47058.
KOiK15441.
OMAiNERAPKP.
OrthoDBiEOG74FFC7.

Enzyme and pathway databases

BioCyciYEAST:YJL035C-MONOMER.
BRENDAi3.5.4.33. 984.

Miscellaneous databases

PROiP47058.

Family and domain databases

InterProiIPR016192. APOBEC/CMP_deaminase_Zn-bd.
IPR002125. CMP_dCMP_Zn-bd.
IPR016193. Cytidine_deaminase-like.
[Graphical view]
PfamiPF00383. dCMP_cyt_deam_1. 1 hit.
[Graphical view]
SUPFAMiSSF53927. SSF53927. 1 hit.
PROSITEiPS00903. CYT_DCMP_DEAMINASES_1. 1 hit.
PS51747. CYT_DCMP_DEAMINASES_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "An adenosine deaminase that generates inosine at the wobble position of transfer RNAs."
    Gerber A.P., Keller W.
    Science 286:1146-1149(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
    Strain: BMA41.
  2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Sora S., Tiboni O., Sanangelantoni A.M.
    Submitted (FEB-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 12-250.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTAD2_YEAST
AccessioniPrimary (citable) accession number: P47058
Secondary accession number(s): D6VWE8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: July 6, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 830 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.