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Protein

tRNA-specific adenosine deaminase subunit TAD2

Gene

TAD2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Deaminates adenosine-34 to inosine in many tRNAs.

Miscellaneous

Present with 830 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Adenine(34) in tRNA + H2O = hypoxanthine(34) in tRNA + NH3.

Cofactori

Zn2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi54Zinc; catalyticBy similarity1
Active sitei56Proton donorBy similarity1
Metal bindingi88Zinc; catalyticBy similarity1
Metal bindingi91Zinc; catalyticBy similarity1

GO - Molecular functioni

GO - Biological processi

  • tRNA modification Source: SGD

Keywordsi

Molecular functionHydrolase
Biological processtRNA processing
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:YJL035C-MONOMER
BRENDAi3.5.4.33 984

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-specific adenosine deaminase subunit TAD2 (EC:3.5.4.33)
Alternative name(s):
tRNA-specific adenosine-34 deaminase subunit TAD2
Gene namesi
Name:TAD2
Ordered Locus Names:YJL035C
ORF Names:J1246
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJL035C
SGDiS000003572 TAD2

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001717401 – 250tRNA-specific adenosine deaminase subunit TAD2Add BLAST250

Proteomic databases

PaxDbiP47058
PRIDEiP47058

Interactioni

Subunit structurei

Heterodimer with TAD3.

Binary interactionsi

WithEntry#Exp.IntActNotes
TAD3Q9URQ32EBI-18939,EBI-2094330

Protein-protein interaction databases

BioGridi33725, 90 interactors
DIPiDIP-5546N
IntActiP47058, 3 interactors
MINTiP47058
STRINGi4932.YJL035C

Structurei

3D structure databases

ProteinModelPortaliP47058
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 119CMP/dCMP-type deaminasePROSITE-ProRule annotationAdd BLAST119

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000000280
HOGENOMiHOG000085051
InParanoidiP47058
KOiK15441
OMAiGMNDTNR
OrthoDBiEOG092C3SV3

Family and domain databases

InterProiView protein in InterPro
IPR016192 APOBEC/CMP_deaminase_Zn-bd
IPR002125 CMP_dCMP_dom
IPR016193 Cytidine_deaminase-like
PfamiView protein in Pfam
PF00383 dCMP_cyt_deam_1, 1 hit
SUPFAMiSSF53927 SSF53927, 1 hit
PROSITEiView protein in PROSITE
PS00903 CYT_DCMP_DEAMINASES_1, 1 hit
PS51747 CYT_DCMP_DEAMINASES_2, 1 hit

Sequencei

Sequence statusi: Complete.

P47058-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQHIKHMRTA VRLARYALDH DETPVACIFV HTPTGQVMAY GMNDTNKSLT
60 70 80 90 100
GVAHAEFMGI DQIKAMLGSR GVVDVFKDIT LYVTVEPCIM CASALKQLDI
110 120 130 140 150
GKVVFGCGNE RFGGNGTVLS VNHDTCTLVP KNNSAAGYES IPGILRKEAI
160 170 180 190 200
MLLRYFYVRQ NERAPKPRSK SDRVLDKNTF PPMEWSKYLN EEAFIETFGD
210 220 230 240 250
DYRTCFANKV DLSSNSVDWD LIDSHQDNII QELEEQCKMF KFNVHKKSKV
Length:250
Mass (Da):28,252
Last modified:February 1, 1996 - v1
Checksum:iC774A1E50AA68106
GO

Sequence cautioni

The sequence CAA88261 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ242667 Genomic DNA Translation: CAB60629.1
Z49310 Genomic DNA Translation: CAA89326.1
AY693172 Genomic DNA Translation: AAT93191.1
Z48229 Genomic DNA Translation: CAA88261.1 Different initiation.
BK006943 Genomic DNA Translation: DAA08764.1
PIRiS56807
RefSeqiNP_012499.1, NM_001181469.1

Genome annotation databases

EnsemblFungiiYJL035C; YJL035C; YJL035C
GeneIDi853417
KEGGisce:YJL035C

Similar proteinsi

Entry informationi

Entry nameiTAD2_YEAST
AccessioniPrimary (citable) accession number: P47058
Secondary accession number(s): D6VWE8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: March 28, 2018
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health