ID   IRC18_YEAST             Reviewed;         224 AA.
AC   P47056; D6VWE6;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   27-MAR-2024, entry version 131.
DE   RecName: Full=Outer spore wall protein 6 {ECO:0000303|PubMed:23966878};
DE   AltName: Full=Increased recombination centers protein 18 {ECO:0000303|PubMed:18085829};
DE   Flags: Precursor;
GN   Name=IRC18 {ECO:0000303|PubMed:18085829};
GN   Synonyms=OSW6 {ECO:0000303|PubMed:23966878};
GN   OrderedLocusNames=YJL037W {ECO:0000312|SGD:S000003574};
GN   ORFNames=J1234;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=8641269; DOI=10.1002/j.1460-2075.1996.tb00557.x;
RA   Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C.,
RA   Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D.,
RA   Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J.,
RA   Heumann K., Hilger F., Hollenberg C.P., Huang M.-E., Jacq C.,
RA   Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E.,
RA   Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T.,
RA   Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B.,
RA   Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R.,
RA   Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N.,
RA   To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H.,
RA   von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.;
RT   "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.";
RL   EMBO J. 15:2031-2049(1996).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [4]
RP   INDUCTION.
RX   PubMed=11179416; DOI=10.1091/mbc.12.2.297;
RA   Epstein C.B., Waddle J.A., Hale W. IV, Dave V., Thornton J., Macatee T.L.,
RA   Garner H.R., Butow R.A.;
RT   "Genome-wide responses to mitochondrial dysfunction.";
RL   Mol. Biol. Cell 12:297-308(2001).
RN   [5]
RP   INDUCTION.
RX   PubMed=14561723; DOI=10.1093/jb/mvg155;
RA   Sakaki K., Tashiro K., Kuhara S., Mihara K.;
RT   "Response of genes associated with mitochondrial function to mild heat
RT   stress in yeast Saccharomyces cerevisiae.";
RL   J. Biochem. 134:373-384(2003).
RN   [6]
RP   DISRUPTION PHENOTYPE.
RX   PubMed=18085829; DOI=10.1371/journal.pgen.0030228;
RA   Alvaro D., Lisby M., Rothstein R.;
RT   "Genome-wide analysis of Rad52 foci reveals diverse mechanisms impacting
RT   recombination.";
RL   PLoS Genet. 3:E228-E228(2007).
RN   [7]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=23966878; DOI=10.1371/journal.pgen.1003700;
RA   Lin C.P., Kim C., Smith S.O., Neiman A.M.;
RT   "A highly redundant gene network controls assembly of the outer spore wall
RT   in S. cerevisiae.";
RL   PLoS Genet. 9:E1003700-E1003700(2013).
RN   [8]
RP   IDENTIFICATION BY MASS SPECTROMETRY.
RX   PubMed=29897761; DOI=10.1021/acs.jproteome.8b00032;
RA   He C., Jia C., Zhang Y., Xu P.;
RT   "Enrichment-based proteogenomics identifies microproteins, missing
RT   proteins, and novel smORFs in Saccharomyces cerevisiae.";
RL   J. Proteome Res. 17:2335-2344(2018).
CC   -!- FUNCTION: Involved in spore wall assembly.
CC       {ECO:0000269|PubMed:23966878}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- INDUCTION: In respiratory-deficient cells and by heat.
CC       {ECO:0000269|PubMed:11179416, ECO:0000269|PubMed:14561723}.
CC   -!- DISRUPTION PHENOTYPE: Displays increased levels of spontaneous RAD52
CC       foci in proliferating diploid cells (PubMed:18085829). A combined
CC       deletion of the LOH1/OSW4 and IRC18/OSW6 has reduced dityrosine
CC       incorporation in the outer spore wall (PubMed:23966878).
CC       {ECO:0000269|PubMed:18085829, ECO:0000269|PubMed:23966878}.
CC   -!- SIMILARITY: Belongs to the OSW4/6 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; Z49312; CAA89328.1; -; Genomic_DNA.
DR   EMBL; AY558244; AAS56570.1; -; Genomic_DNA.
DR   EMBL; BK006943; DAA08762.1; -; Genomic_DNA.
DR   PIR; S56809; S56809.
DR   RefSeq; NP_012497.3; NM_001181471.3.
DR   AlphaFoldDB; P47056; -.
DR   SMR; P47056; -.
DR   BioGRID; 33722; 21.
DR   STRING; 4932.YJL037W; -.
DR   GlyCosmos; P47056; 2 sites, No reported glycans.
DR   GlyGen; P47056; 2 sites.
DR   iPTMnet; P47056; -.
DR   PaxDb; 4932-YJL037W; -.
DR   EnsemblFungi; YJL037W_mRNA; YJL037W; YJL037W.
DR   GeneID; 853414; -.
DR   KEGG; sce:YJL037W; -.
DR   AGR; SGD:S000003574; -.
DR   SGD; S000003574; IRC18.
DR   VEuPathDB; FungiDB:YJL037W; -.
DR   GeneTree; ENSGT00940000182578; -.
DR   HOGENOM; CLU_107727_0_0_1; -.
DR   InParanoid; P47056; -.
DR   OMA; GEXTEEQ; -.
DR   OrthoDB; 2023500at2759; -.
DR   BioCyc; YEAST:G3O-31503-MONOMER; -.
DR   BioGRID-ORCS; 853414; 0 hits in 10 CRISPR screens.
DR   PRO; PR:P47056; -.
DR   Proteomes; UP000002311; Chromosome X.
DR   RNAct; P47056; Protein.
DR   GO; GO:0000324; C:fungal-type vacuole; HDA:SGD.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0030476; P:ascospore wall assembly; IGI:SGD.
PE   1: Evidence at protein level;
KW   Glycoprotein; Membrane; Reference proteome; Signal; Sporulation;
KW   Transmembrane; Transmembrane helix.
FT   SIGNAL          1..26
FT                   /evidence="ECO:0000255"
FT   CHAIN           27..224
FT                   /note="Outer spore wall protein 6"
FT                   /id="PRO_0000203069"
FT   TOPO_DOM        27..174
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000250|UniProtKB:P47055"
FT   TRANSMEM        175..195
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        196..199
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:P47055"
FT   TRANSMEM        200..220
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        221..224
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000250|UniProtKB:P47055"
FT   CARBOHYD        74
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        104
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ   SEQUENCE   224 AA;  24746 MW;  6C17841176EADC1F CRC64;
     MKVQMIERIF LIQLCLLTVV LASSRAVVEF ESTGTKLVNS LRVLAAYSQS SVCVDEKISG
     IERQIEEVKD MYGNHSFILK GLNGILNNKV NMLTREIQME TVGNNTFETE TGKLTKGLNR
     AVNISPFKYI KKFKTVSTKK FESLLNKYDL VAKKGGELTE EQKKKKEVLS RISRVVAATT
     IEAGLAQGVV DLCITVTTSL CLVSASIGGV GFLIWLTIIY QALT
//