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Protein

Succinate dehydrogenase [ubiquinone] flavoprotein subunit 2, mitochondrial

Gene

YJL045W

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Probable minor catalytic subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Probably forms a catalytic dimer with SDH2. Electrons flow from succinate to the FAD bound to the catalytic subunit, and sequentially through the iron-sulfur clusters bound to SDH2 and enter the membrane dimer formed by SDH3 and SDH4.1 Publication

Miscellaneous

In vitro, can complement a SDH1 disruption and leads to less than 15% of wild-type SDH reductase activity probably due to its lower expression level (compared to SDH1).

Catalytic activityi

Succinate + a quinone = fumarate + a quinol.By similarity

Cofactori

FADBy similarity

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes fumarate from succinate (eukaryal route).By similarity
Proteins known to be involved in this subpathway in this organism are:
  1. Succinate dehydrogenase [ubiquinone] flavoprotein subunit 2, mitochondrial (YJL045W), Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (SDH2), Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial (SDH1)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes fumarate from succinate (eukaryal route), the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei260FADBy similarity1
Binding sitei281SubstrateBy similarity1
Binding sitei293SubstrateBy similarity1
Active sitei325Proton acceptorBy similarity1
Binding sitei392SubstrateBy similarity1
Binding sitei427FADBy similarity1
Binding sitei438SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi53 – 58FADBy similarity6
Nucleotide bindingi76 – 91FADBy similarityAdd BLAST16
Nucleotide bindingi443 – 444FADBy similarity2

GO - Molecular functioni

  • electron transfer activity Source: GO_Central
  • flavin adenine dinucleotide binding Source: InterPro
  • succinate dehydrogenase (ubiquinone) activity Source: SGD

GO - Biological processi

  • anaerobic respiration Source: GO_Central
  • cellular respiration Source: SGD
  • mitochondrial electron transport, succinate to ubiquinone Source: GO_Central
  • tricarboxylic acid cycle Source: GO_Central

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Transport, Tricarboxylic acid cycle
LigandFAD, Flavoprotein

Enzyme and pathway databases

BioCyciYEAST:YJL045W-MONOMER
UniPathwayiUPA00223; UER01006

Names & Taxonomyi

Protein namesi
Recommended name:
Succinate dehydrogenase [ubiquinone] flavoprotein subunit 2, mitochondrial (EC:1.3.5.1By similarity)
Alternative name(s):
Flavoprotein subunit of complex II
Short name:
FP
SDH1b
Gene namesi
Ordered Locus Names:YJL045W
ORF Names:J1194
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJL045W
SGDiS000003581 YJL045W

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 25MitochondrionSequence analysisAdd BLAST25
ChainiPRO_000001034326 – 634Succinate dehydrogenase [ubiquinone] flavoprotein subunit 2, mitochondrialAdd BLAST609

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei84Tele-8alpha-FAD histidineBy similarity1

Proteomic databases

MaxQBiP47052
PaxDbiP47052
PRIDEiP47052

PTM databases

iPTMnetiP47052

Interactioni

Subunit structurei

Component of complex II composed of four subunits: a flavoprotein (FP), an iron-sulfur protein (IP), and a cytochrome b composed of a large and a small subunit.By similarity

Protein-protein interaction databases

BioGridi33713, 259 interactors
DIPiDIP-4262N
IntActiP47052, 3 interactors
MINTiP47052
STRINGi4932.YJL045W

Structurei

3D structure databases

ProteinModelPortaliP47052
SMRiP47052
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00910000144277
HOGENOMiHOG000160475
InParanoidiP47052
KOiK00234
OMAiCANNILE
OrthoDBiEOG092C17LB

Family and domain databases

Gene3Di3.50.50.60, 2 hits
3.90.700.10, 1 hit
InterProiView protein in InterPro
IPR003953 FAD-binding_2
IPR036188 FAD/NAD-bd_sf
IPR003952 FRD_SDH_FAD_BS
IPR037099 Fum_R/Succ_DH_flav-like_C_sf
IPR015939 Fum_Rdtase/Succ_DH_flav-like_C
IPR027477 Succ_DH/fumarate_Rdtase_cat_sf
IPR011281 Succ_DH_flav_su_fwd
IPR014006 Succ_Dhase_FrdA_Gneg
PfamiView protein in Pfam
PF00890 FAD_binding_2, 1 hit
PF02910 Succ_DH_flav_C, 1 hit
SUPFAMiSSF46977 SSF46977, 1 hit
SSF51905 SSF51905, 2 hits
SSF56425 SSF56425, 1 hit
TIGRFAMsiTIGR01816 sdhA_forward, 1 hit
TIGR01812 sdhA_frdA_Gneg, 1 hit
PROSITEiView protein in PROSITE
PS00504 FRD_SDH_FAD_BINDING, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P47052-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSLKKGITK SYILQRTFTS SSVVRQIGEV KSESKPPAKY HIIDHEYDCV
60 70 80 90 100
VVGAGGAGLR AAFGLAEAGY KTACLSKLFP TRSHTVAAQG GINAALGNMH
110 120 130 140 150
PDDWKSHMYD TVKGSDWLGD QDAIHYMTRE APKSVIELEH YGMPFSRTED
160 170 180 190 200
GRIYQRAFGG QSKDFGKGGQ AYRTCAVADR TGHAMLHTLY GQALKNNTHF
210 220 230 240 250
FIEYFAMDLL THNGEVVGVI AYNQEDGTIH RFRAHKTVIA TGGYGRAYFS
260 270 280 290 300
CTSAHTCTGD GNAMVSRAGF PLEDLEFVQF HPSGIYGSGC LITEGARGEG
310 320 330 340 350
GFLLNSEGER FMERYAPTAK DLASRDVVSR AITMEIRAGR GVGKNKDHIL
360 370 380 390 400
LQLSHLPPEV LKERLPGISE TAAVFAGVDV TQEPIPVLPT VHYNMGGIPT
410 420 430 440 450
KWTGEALTID EETGEDKVIP GLMACGEAAC VSVHGANRLG ANSLLDLVVF
460 470 480 490 500
GRAVANTIAD TLQPGLPHKP LASNIGHESI ANLDKVRNAR GSLKTSQIRL
510 520 530 540 550
NMQRTMQKDV SVFRTQDTLD EGVRNITEVD KTFEDVHVSD KSMIWNSDLV
560 570 580 590 600
ETLELQNLLT CATQTAVSAS KRKESRGAHA REDYAKRDDV NWRKHTLSWQ
610 620 630
KGTSTPVKIK YRNVIAHTLD ENECAPVPPA VRSY
Length:634
Mass (Da):69,382
Last modified:February 1, 1996 - v1
Checksum:iF00CD157AB9BF9C5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49320 Genomic DNA Translation: CAA89336.1
BK006943 Genomic DNA Translation: DAA08755.1
PIRiS56817
RefSeqiNP_012490.1, NM_001181478.1

Genome annotation databases

EnsemblFungiiYJL045W; YJL045W; YJL045W
GeneIDi853405
KEGGisce:YJL045W

Similar proteinsi

Entry informationi

Entry nameiSDHX_YEAST
AccessioniPrimary (citable) accession number: P47052
Secondary accession number(s): D6VWD9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: May 23, 2018
This is version 163 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

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