Succinate dehydrogenase [ubiquinone] flavoprotein subunit 2, mitochondrial precursor - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P47052 (DHSX_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 115. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Succinate dehydrogenase [ubiquinone] flavoprotein subunit 2, mitochondrial
EC=1.3.5.1

Alternative name(s):
Flavoprotein subunit of complex II
Short name=FP
SDH1b

Gene names

Ordered Locus Names:	YJL045W
ORF Names:	J1194

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	634 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable minor catalytic subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Probably forms a catalytic dimer with SDH2. Electrons flow from succinate to the FAD bound to the catalytic subunit, and sequentially through the iron-sulfur clusters bound to SDH2 and enter the membrane dimer formed by SDH3 and SDH4. Ref.3
Catalytic activity	Succinate + ubiquinone = fumarate + ubiquinol.
Cofactor	FAD.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (eukaryal route): step 1/1.
Subunit structure	Component of complex II composed of four subunits: a flavoprotein (FP), an iron-sulfur protein (IP), and a cytochrome b composed of a large and a small subunit By similarity.
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.
Miscellaneous	In vitro, can complement a SDH1 disruption and leads to less than 15% of wild-type SDH reductase activity probably due to its lower expression level (compared to SDH1).
Sequence similarities	Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.

Ontologies

Keywords
Biological process	Electron transport Transport Tricarboxylic acid cycle
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular respiration Inferred from mutant phenotype PubMed 16232921. Source: SGD electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrion Inferred from direct assay PubMed 14576278 PubMed 16823961. Source: SGD
Molecular_function	flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro succinate dehydrogenase (ubiquinone) activity Inferred from mutant phenotype PubMed 16232921. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 25

Mitochondrion Potential

Chain

26 – 634

609

Succinate dehydrogenase [ubiquinone] flavoprotein subunit 2, mitochondrial

PRO_0000010343

Regions

Nucleotide binding

53 – 58

FAD By similarity

Nucleotide binding

76 – 91

FAD By similarity

Nucleotide binding

443 – 444

FAD By similarity

Sites

Active site

325

Proton acceptor By similarity

Binding site

260

FAD By similarity

Binding site

281

Substrate By similarity

Binding site

293

Substrate By similarity

Binding site

392

Substrate By similarity

Binding site

427

FAD By similarity

Binding site

438

Substrate By similarity

Amino acid modifications

Modified residue

Tele-8alpha-FAD histidine By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P47052 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: F00CD157AB9BF9C5
Show »

FASTA

634

69,382

        10         20         30         40         50         60 
MLSLKKGITK SYILQRTFTS SSVVRQIGEV KSESKPPAKY HIIDHEYDCV VVGAGGAGLR 

        70         80         90        100        110        120 
AAFGLAEAGY KTACLSKLFP TRSHTVAAQG GINAALGNMH PDDWKSHMYD TVKGSDWLGD 

       130        140        150        160        170        180 
QDAIHYMTRE APKSVIELEH YGMPFSRTED GRIYQRAFGG QSKDFGKGGQ AYRTCAVADR 

       190        200        210        220        230        240 
TGHAMLHTLY GQALKNNTHF FIEYFAMDLL THNGEVVGVI AYNQEDGTIH RFRAHKTVIA 

       250        260        270        280        290        300 
TGGYGRAYFS CTSAHTCTGD GNAMVSRAGF PLEDLEFVQF HPSGIYGSGC LITEGARGEG 

       310        320        330        340        350        360 
GFLLNSEGER FMERYAPTAK DLASRDVVSR AITMEIRAGR GVGKNKDHIL LQLSHLPPEV 

       370        380        390        400        410        420 
LKERLPGISE TAAVFAGVDV TQEPIPVLPT VHYNMGGIPT KWTGEALTID EETGEDKVIP 

       430        440        450        460        470        480 
GLMACGEAAC VSVHGANRLG ANSLLDLVVF GRAVANTIAD TLQPGLPHKP LASNIGHESI 

       490        500        510        520        530        540 
ANLDKVRNAR GSLKTSQIRL NMQRTMQKDV SVFRTQDTLD EGVRNITEVD KTFEDVHVSD 

       550        560        570        580        590        600 
KSMIWNSDLV ETLELQNLLT CATQTAVSAS KRKESRGAHA REDYAKRDDV NWRKHTLSWQ 

       610        620        630 
KGTSTPVKIK YRNVIAHTLD ENECAPVPPA VRSY

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Suppression of sdh1 mutations by the SDH1b gene of Saccharomyces cerevisiae." Colby G., Ishii Y., Tzagoloff A. Yeast 14:1001-1006(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z49320 Genomic DNA. Translation: CAA89336.1. BK006943 Genomic DNA. Translation: DAA08755.1.
PIR	S56817.
RefSeq	NP_012490.1. NM_001181478.1.
3D structure databases
ProteinModelPortal	P47052.
SMR	P47052. Positions 39-634.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4262N.
IntAct	P47052. 1 interaction.
MINT	MINT-501076.
STRING	4932.YJL045W.
Proteomic databases
PaxDb	P47052.
PeptideAtlas	P47052.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJL045W; YJL045W; YJL045W.
GeneID	853405.
KEGG	sce:YJL045W.
Organism-specific databases
CYGD	YJL045w.
SGD	S000003581. YJL045W.
Phylogenomic databases
eggNOG	COG1053.
GeneTree	ENSGT00390000010046.
HOGENOM	HOG000160475.
KO	K00234.
OMA	HAMRSHS.
OrthoDB	EOG41G6C9.
Enzyme and pathway databases
UniPathway	UPA00223; UER01006.
Gene expression databases
Genevestigator	P47052.
GermOnline	YJL045W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.20.58.100. 1 hit.
InterPro	IPR003953. FAD_bind_dom. IPR003952. FRD_SDH_FAD_BS. IPR015939. Fum_Rdtase/Succ_DH_flav-like_C. IPR011281. Succ_DH_flav_su_fwd. IPR014006. Succ_Dhase_FrdA_Gneg. [Graphical view]
PANTHER	PTHR11632:SF5. PTHR11632:SF5. 1 hit.
Pfam	PF00890. FAD_binding_2. 1 hit. PF02910. Succ_DH_flav_C. 1 hit. [Graphical view]
SUPFAM	SSF46977. Succ_DH_flav_C. 1 hit.
TIGRFAMs	TIGR01816. sdhA_forward. 1 hit. TIGR01812. sdhA_frdA_Gneg. 1 hit.
PROSITE	PS00504. FRD_SDH_FAD_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	973903.

Entry information

Entry name

DHSX_YEAST

Accession

Primary (citable) accession number: P47052
Secondary accession number(s): D6VWD9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	April 3, 2013
This is version 115 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents