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P47052

- SDHX_YEAST

UniProt

P47052 - SDHX_YEAST

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Protein

Succinate dehydrogenase [ubiquinone] flavoprotein subunit 2, mitochondrial

Gene

YJL045W

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Probable minor catalytic subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Probably forms a catalytic dimer with SDH2. Electrons flow from succinate to the FAD bound to the catalytic subunit, and sequentially through the iron-sulfur clusters bound to SDH2 and enter the membrane dimer formed by SDH3 and SDH4.1 Publication

Catalytic activityi

Succinate + a quinone = fumarate + a quinol.

Cofactori

FAD.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei260 – 2601FADBy similarity
Binding sitei281 – 2811SubstrateBy similarity
Binding sitei293 – 2931SubstrateBy similarity
Active sitei325 – 3251Proton acceptorBy similarity
Binding sitei392 – 3921SubstrateBy similarity
Binding sitei427 – 4271FADBy similarity
Binding sitei438 – 4381SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi53 – 586FADBy similarity
Nucleotide bindingi76 – 9116FADBy similarityAdd
BLAST
Nucleotide bindingi443 – 4442FADBy similarity

GO - Molecular functioni

  1. flavin adenine dinucleotide binding Source: InterPro
  2. succinate dehydrogenase (ubiquinone) activity Source: SGD

GO - Biological processi

  1. cellular respiration Source: SGD
  2. electron transport chain Source: InterPro
  3. tricarboxylic acid cycle Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Transport, Tricarboxylic acid cycle

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

UniPathwayiUPA00223; UER01006.

Names & Taxonomyi

Protein namesi
Recommended name:
Succinate dehydrogenase [ubiquinone] flavoprotein subunit 2, mitochondrial (EC:1.3.5.1)
Alternative name(s):
Flavoprotein subunit of complex II
Short name:
FP
SDH1b
Gene namesi
Ordered Locus Names:YJL045W
ORF Names:J1194
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

CYGDiYJL045w.
SGDiS000003581. YJL045W.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial inner membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2525MitochondrionSequence AnalysisAdd
BLAST
Chaini26 – 634609Succinate dehydrogenase [ubiquinone] flavoprotein subunit 2, mitochondrialPRO_0000010343Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei84 – 841Tele-8alpha-FAD histidineBy similarity

Proteomic databases

PaxDbiP47052.
PeptideAtlasiP47052.
PRIDEiP47052.

Expressioni

Gene expression databases

GenevestigatoriP47052.

Interactioni

Subunit structurei

Component of complex II composed of four subunits: a flavoprotein (FP), an iron-sulfur protein (IP), and a cytochrome b composed of a large and a small subunit.By similarity

Protein-protein interaction databases

BioGridi33713. 88 interactions.
DIPiDIP-4262N.
IntActiP47052. 1 interaction.
MINTiMINT-501076.
STRINGi4932.YJL045W.

Structurei

3D structure databases

ProteinModelPortaliP47052.
SMRiP47052. Positions 39-634.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG1053.
GeneTreeiENSGT00390000010046.
HOGENOMiHOG000160475.
InParanoidiP47052.
KOiK00234.
OMAiETIRWAE.
OrthoDBiEOG7V76G3.

Family and domain databases

Gene3Di1.20.58.100. 1 hit.
3.90.700.10. 1 hit.
InterProiIPR003953. FAD_bind_dom.
IPR003952. FRD_SDH_FAD_BS.
IPR015939. Fum_Rdtase/Succ_DH_flav-like_C.
IPR027477. Succ_DH/fumarate_Rdtase_cat.
IPR011281. Succ_DH_flav_su_fwd.
IPR014006. Succ_Dhase_FrdA_Gneg.
[Graphical view]
PfamiPF00890. FAD_binding_2. 1 hit.
PF02910. Succ_DH_flav_C. 1 hit.
[Graphical view]
SUPFAMiSSF46977. SSF46977. 1 hit.
SSF56425. SSF56425. 1 hit.
TIGRFAMsiTIGR01816. sdhA_forward. 1 hit.
TIGR01812. sdhA_frdA_Gneg. 1 hit.
PROSITEiPS00504. FRD_SDH_FAD_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P47052-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLSLKKGITK SYILQRTFTS SSVVRQIGEV KSESKPPAKY HIIDHEYDCV
60 70 80 90 100
VVGAGGAGLR AAFGLAEAGY KTACLSKLFP TRSHTVAAQG GINAALGNMH
110 120 130 140 150
PDDWKSHMYD TVKGSDWLGD QDAIHYMTRE APKSVIELEH YGMPFSRTED
160 170 180 190 200
GRIYQRAFGG QSKDFGKGGQ AYRTCAVADR TGHAMLHTLY GQALKNNTHF
210 220 230 240 250
FIEYFAMDLL THNGEVVGVI AYNQEDGTIH RFRAHKTVIA TGGYGRAYFS
260 270 280 290 300
CTSAHTCTGD GNAMVSRAGF PLEDLEFVQF HPSGIYGSGC LITEGARGEG
310 320 330 340 350
GFLLNSEGER FMERYAPTAK DLASRDVVSR AITMEIRAGR GVGKNKDHIL
360 370 380 390 400
LQLSHLPPEV LKERLPGISE TAAVFAGVDV TQEPIPVLPT VHYNMGGIPT
410 420 430 440 450
KWTGEALTID EETGEDKVIP GLMACGEAAC VSVHGANRLG ANSLLDLVVF
460 470 480 490 500
GRAVANTIAD TLQPGLPHKP LASNIGHESI ANLDKVRNAR GSLKTSQIRL
510 520 530 540 550
NMQRTMQKDV SVFRTQDTLD EGVRNITEVD KTFEDVHVSD KSMIWNSDLV
560 570 580 590 600
ETLELQNLLT CATQTAVSAS KRKESRGAHA REDYAKRDDV NWRKHTLSWQ
610 620 630
KGTSTPVKIK YRNVIAHTLD ENECAPVPPA VRSY
Length:634
Mass (Da):69,382
Last modified:February 1, 1996 - v1
Checksum:iF00CD157AB9BF9C5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z49320 Genomic DNA. Translation: CAA89336.1.
BK006943 Genomic DNA. Translation: DAA08755.1.
PIRiS56817.
RefSeqiNP_012490.1. NM_001181478.1.

Genome annotation databases

EnsemblFungiiYJL045W; YJL045W; YJL045W.
GeneIDi853405.
KEGGisce:YJL045W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z49320 Genomic DNA. Translation: CAA89336.1 .
BK006943 Genomic DNA. Translation: DAA08755.1 .
PIRi S56817.
RefSeqi NP_012490.1. NM_001181478.1.

3D structure databases

ProteinModelPortali P47052.
SMRi P47052. Positions 39-634.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33713. 88 interactions.
DIPi DIP-4262N.
IntActi P47052. 1 interaction.
MINTi MINT-501076.
STRINGi 4932.YJL045W.

Proteomic databases

PaxDbi P47052.
PeptideAtlasi P47052.
PRIDEi P47052.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YJL045W ; YJL045W ; YJL045W .
GeneIDi 853405.
KEGGi sce:YJL045W.

Organism-specific databases

CYGDi YJL045w.
SGDi S000003581. YJL045W.

Phylogenomic databases

eggNOGi COG1053.
GeneTreei ENSGT00390000010046.
HOGENOMi HOG000160475.
InParanoidi P47052.
KOi K00234.
OMAi ETIRWAE.
OrthoDBi EOG7V76G3.

Enzyme and pathway databases

UniPathwayi UPA00223 ; UER01006 .

Miscellaneous databases

NextBioi 973903.

Gene expression databases

Genevestigatori P47052.

Family and domain databases

Gene3Di 1.20.58.100. 1 hit.
3.90.700.10. 1 hit.
InterProi IPR003953. FAD_bind_dom.
IPR003952. FRD_SDH_FAD_BS.
IPR015939. Fum_Rdtase/Succ_DH_flav-like_C.
IPR027477. Succ_DH/fumarate_Rdtase_cat.
IPR011281. Succ_DH_flav_su_fwd.
IPR014006. Succ_Dhase_FrdA_Gneg.
[Graphical view ]
Pfami PF00890. FAD_binding_2. 1 hit.
PF02910. Succ_DH_flav_C. 1 hit.
[Graphical view ]
SUPFAMi SSF46977. SSF46977. 1 hit.
SSF56425. SSF56425. 1 hit.
TIGRFAMsi TIGR01816. sdhA_forward. 1 hit.
TIGR01812. sdhA_frdA_Gneg. 1 hit.
PROSITEi PS00504. FRD_SDH_FAD_BINDING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Suppression of sdh1 mutations by the SDH1b gene of Saccharomyces cerevisiae."
    Colby G., Ishii Y., Tzagoloff A.
    Yeast 14:1001-1006(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiSDHX_YEAST
AccessioniPrimary (citable) accession number: P47052
Secondary accession number(s): D6VWD9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: October 29, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

In vitro, can complement a SDH1 disruption and leads to less than 15% of wild-type SDH reductase activity probably due to its lower expression level (compared to SDH1).

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3