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P47052

- SDHX_YEAST

UniProt

P47052 - SDHX_YEAST

Protein

Succinate dehydrogenase [ubiquinone] flavoprotein subunit 2, mitochondrial

Gene

YJL045W

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 126 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Probable minor catalytic subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Probably forms a catalytic dimer with SDH2. Electrons flow from succinate to the FAD bound to the catalytic subunit, and sequentially through the iron-sulfur clusters bound to SDH2 and enter the membrane dimer formed by SDH3 and SDH4.1 Publication

    Catalytic activityi

    Succinate + a quinone = fumarate + a quinol.

    Cofactori

    FAD.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei260 – 2601FADBy similarity
    Binding sitei281 – 2811SubstrateBy similarity
    Binding sitei293 – 2931SubstrateBy similarity
    Active sitei325 – 3251Proton acceptorBy similarity
    Binding sitei392 – 3921SubstrateBy similarity
    Binding sitei427 – 4271FADBy similarity
    Binding sitei438 – 4381SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi53 – 586FADBy similarity
    Nucleotide bindingi76 – 9116FADBy similarityAdd
    BLAST
    Nucleotide bindingi443 – 4442FADBy similarity

    GO - Molecular functioni

    1. flavin adenine dinucleotide binding Source: InterPro
    2. succinate dehydrogenase (ubiquinone) activity Source: SGD

    GO - Biological processi

    1. cellular respiration Source: SGD
    2. electron transport chain Source: InterPro
    3. tricarboxylic acid cycle Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Transport, Tricarboxylic acid cycle

    Keywords - Ligandi

    FAD, Flavoprotein

    Enzyme and pathway databases

    UniPathwayiUPA00223; UER01006.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Succinate dehydrogenase [ubiquinone] flavoprotein subunit 2, mitochondrial (EC:1.3.5.1)
    Alternative name(s):
    Flavoprotein subunit of complex II
    Short name:
    FP
    SDH1b
    Gene namesi
    Ordered Locus Names:YJL045W
    ORF Names:J1194
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome X

    Organism-specific databases

    CYGDiYJL045w.
    SGDiS000003581. YJL045W.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial inner membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2525MitochondrionSequence AnalysisAdd
    BLAST
    Chaini26 – 634609Succinate dehydrogenase [ubiquinone] flavoprotein subunit 2, mitochondrialPRO_0000010343Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei84 – 841Tele-8alpha-FAD histidineBy similarity

    Proteomic databases

    PaxDbiP47052.
    PeptideAtlasiP47052.
    PRIDEiP47052.

    Expressioni

    Gene expression databases

    GenevestigatoriP47052.

    Interactioni

    Subunit structurei

    Component of complex II composed of four subunits: a flavoprotein (FP), an iron-sulfur protein (IP), and a cytochrome b composed of a large and a small subunit.By similarity

    Protein-protein interaction databases

    BioGridi33713. 88 interactions.
    DIPiDIP-4262N.
    IntActiP47052. 1 interaction.
    MINTiMINT-501076.
    STRINGi4932.YJL045W.

    Structurei

    3D structure databases

    ProteinModelPortaliP47052.
    SMRiP47052. Positions 39-634.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG1053.
    GeneTreeiENSGT00390000010046.
    HOGENOMiHOG000160475.
    KOiK00234.
    OMAiETIRWAE.
    OrthoDBiEOG7V76G3.

    Family and domain databases

    Gene3Di1.20.58.100. 1 hit.
    3.90.700.10. 1 hit.
    InterProiIPR003953. FAD_bind_dom.
    IPR003952. FRD_SDH_FAD_BS.
    IPR015939. Fum_Rdtase/Succ_DH_flav-like_C.
    IPR027477. Succ_DH/fumarate_Rdtase_cat.
    IPR011281. Succ_DH_flav_su_fwd.
    IPR014006. Succ_Dhase_FrdA_Gneg.
    [Graphical view]
    PfamiPF00890. FAD_binding_2. 1 hit.
    PF02910. Succ_DH_flav_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF46977. SSF46977. 1 hit.
    SSF56425. SSF56425. 1 hit.
    TIGRFAMsiTIGR01816. sdhA_forward. 1 hit.
    TIGR01812. sdhA_frdA_Gneg. 1 hit.
    PROSITEiPS00504. FRD_SDH_FAD_BINDING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P47052-1 [UniParc]FASTAAdd to Basket

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    MLSLKKGITK SYILQRTFTS SSVVRQIGEV KSESKPPAKY HIIDHEYDCV    50
    VVGAGGAGLR AAFGLAEAGY KTACLSKLFP TRSHTVAAQG GINAALGNMH 100
    PDDWKSHMYD TVKGSDWLGD QDAIHYMTRE APKSVIELEH YGMPFSRTED 150
    GRIYQRAFGG QSKDFGKGGQ AYRTCAVADR TGHAMLHTLY GQALKNNTHF 200
    FIEYFAMDLL THNGEVVGVI AYNQEDGTIH RFRAHKTVIA TGGYGRAYFS 250
    CTSAHTCTGD GNAMVSRAGF PLEDLEFVQF HPSGIYGSGC LITEGARGEG 300
    GFLLNSEGER FMERYAPTAK DLASRDVVSR AITMEIRAGR GVGKNKDHIL 350
    LQLSHLPPEV LKERLPGISE TAAVFAGVDV TQEPIPVLPT VHYNMGGIPT 400
    KWTGEALTID EETGEDKVIP GLMACGEAAC VSVHGANRLG ANSLLDLVVF 450
    GRAVANTIAD TLQPGLPHKP LASNIGHESI ANLDKVRNAR GSLKTSQIRL 500
    NMQRTMQKDV SVFRTQDTLD EGVRNITEVD KTFEDVHVSD KSMIWNSDLV 550
    ETLELQNLLT CATQTAVSAS KRKESRGAHA REDYAKRDDV NWRKHTLSWQ 600
    KGTSTPVKIK YRNVIAHTLD ENECAPVPPA VRSY 634
    Length:634
    Mass (Da):69,382
    Last modified:February 1, 1996 - v1
    Checksum:iF00CD157AB9BF9C5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49320 Genomic DNA. Translation: CAA89336.1.
    BK006943 Genomic DNA. Translation: DAA08755.1.
    PIRiS56817.
    RefSeqiNP_012490.1. NM_001181478.1.

    Genome annotation databases

    EnsemblFungiiYJL045W; YJL045W; YJL045W.
    GeneIDi853405.
    KEGGisce:YJL045W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49320 Genomic DNA. Translation: CAA89336.1 .
    BK006943 Genomic DNA. Translation: DAA08755.1 .
    PIRi S56817.
    RefSeqi NP_012490.1. NM_001181478.1.

    3D structure databases

    ProteinModelPortali P47052.
    SMRi P47052. Positions 39-634.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33713. 88 interactions.
    DIPi DIP-4262N.
    IntActi P47052. 1 interaction.
    MINTi MINT-501076.
    STRINGi 4932.YJL045W.

    Proteomic databases

    PaxDbi P47052.
    PeptideAtlasi P47052.
    PRIDEi P47052.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YJL045W ; YJL045W ; YJL045W .
    GeneIDi 853405.
    KEGGi sce:YJL045W.

    Organism-specific databases

    CYGDi YJL045w.
    SGDi S000003581. YJL045W.

    Phylogenomic databases

    eggNOGi COG1053.
    GeneTreei ENSGT00390000010046.
    HOGENOMi HOG000160475.
    KOi K00234.
    OMAi ETIRWAE.
    OrthoDBi EOG7V76G3.

    Enzyme and pathway databases

    UniPathwayi UPA00223 ; UER01006 .

    Miscellaneous databases

    NextBioi 973903.

    Gene expression databases

    Genevestigatori P47052.

    Family and domain databases

    Gene3Di 1.20.58.100. 1 hit.
    3.90.700.10. 1 hit.
    InterProi IPR003953. FAD_bind_dom.
    IPR003952. FRD_SDH_FAD_BS.
    IPR015939. Fum_Rdtase/Succ_DH_flav-like_C.
    IPR027477. Succ_DH/fumarate_Rdtase_cat.
    IPR011281. Succ_DH_flav_su_fwd.
    IPR014006. Succ_Dhase_FrdA_Gneg.
    [Graphical view ]
    Pfami PF00890. FAD_binding_2. 1 hit.
    PF02910. Succ_DH_flav_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46977. SSF46977. 1 hit.
    SSF56425. SSF56425. 1 hit.
    TIGRFAMsi TIGR01816. sdhA_forward. 1 hit.
    TIGR01812. sdhA_frdA_Gneg. 1 hit.
    PROSITEi PS00504. FRD_SDH_FAD_BINDING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Suppression of sdh1 mutations by the SDH1b gene of Saccharomyces cerevisiae."
      Colby G., Ishii Y., Tzagoloff A.
      Yeast 14:1001-1006(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiSDHX_YEAST
    AccessioniPrimary (citable) accession number: P47052
    Secondary accession number(s): D6VWD9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 126 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    In vitro, can complement a SDH1 disruption and leads to less than 15% of wild-type SDH reductase activity probably due to its lower expression level (compared to SDH1).

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3