Reviewed, UniProtKB/Swiss-Prot P47051 (LPLA_YEAST)

Names and origin

Protein names

Recommended name:
    Putative lipoate-protein ligase A
    EC=2.7.7.63
Alternative name(s):
    Altered inheritance rate of mitochondria protein 22

Gene names

Name:	AIM22
Ordered Locus Names:	YJL046W
ORF Names:	J1171

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	409 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes By similarity.
Catalytic activity	ATP + lipoate = diphosphate + lipoyl-AMP. Lipoyl-AMP + protein = protein N(6)-(lipoyl)lysine + AMP.
Pathway	Protein modification; protein lipoylation via exogenous pathway; protein N(6)-(lipoyl)lysine from lipoic acid: step 1/2. Protein modification; protein lipoylation via exogenous pathway; protein N(6)-(lipoyl)lysine from lipoic acid: step 2/2.
Subunit structure	Monomer By similarity.
Miscellaneous	In the transfer reaction, the free carboxyl group of lipoic acid is attached via an amide linkage to the epsilon-amino group of a specific lysine residue of lipoyl domains of lipoate-dependent enzymes By similarity.
Sequence similarities	Belongs to the lplA family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein lipoylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ligase activity Inferred from electronic annotation. Source: UniProtKB-KW transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 409	409	Putative lipoate-protein ligase A		PRO_0000203066
Regions
	Nucleotide binding	193 – 196	4	ATP By similarity
Sites
	Binding site	188	1	ATP By similarity
	Binding site	249	1	ATP By similarity
	Binding site	249	1	Lipoate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P47051-1 [UniParc]. Last modified June 16, 2009. Version 2. Checksum: 72E9B6D036CDB1E9	FASTA	409	47,003
	10 20 30 40 50 60 MSMMLSNWAL SPRYVGQRNL IHCTTLFHTL TRWAKDADDK YHDINSMYEN MFTPSNDNVS 70 80 90 100 110 120 ILQDEGKSDY DTTKASSMEE DISAFNKDLY NFYNIGYAKQ IMSASQLENI VKAKGRFVIQ 130 140 150 160 170 180 SLSTSPYYNL ALENYVFKNT PRAKRGPDNC RLLFYINDRC AVIGKNQNLW QEVDLAKLKS 190 200 210 220 230 240 KNFELLRRFS GGGTVLHDLG NVNYSYLTSR EKFETKFFNK MIIKWLNSLN PELRLDLNER 250 260 270 280 290 300 GDIIQDGFKI SGSAYKIAGG KAYHHATMLL NADLEQFSGL LEPSLPNNME WESSGVHSVK 310 320 330 340 350 360 SKIKNVGIIT PNQFIAVVSE RFQKTFKVDG EIPIYYCDEF KSINDEIKDA MNTLQSEQWK 370 380 390 400 YFSGPKFSVK IKDKGLTIKV EKGMIYDCDR NDLIGLEFKG FLENIDSYT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Mapping of transcription start sites in Saccharomyces cerevisiae using 5' SAGE." Zhang Z., Dietrich F.S. Nucleic Acids Res. 33:2838-2851(2005) [PubMed: 15905473] [Abstract] Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z49321 Genomic DNA. Translation: CAA89337.1. Different initiation. AY558080 Genomic DNA. Translation: AAS56406.1. Different initiation.
PIR	S56818.
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	YJL046W. Saccharomyces cerevisiae. [Contig view]
GenomeReviews	Gene locus YJL046W in contig Y13136_GR.
KEGG	sce:YJL046W.
NMPDR	fig|4932.3.peg.3463.
Organism-specific databases
CYGD	YJL046w.
SGD	S000003582. AIM22.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P47051.
OMA	P47051. NYSYLTS.
Enzyme and pathway databases
BRENDA	2.7.7.63. 250.
Gene expression databases
ArrayExpress	P47051.
GermOnline	YJL046W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004143. BPL_LipA_LipB. IPR004562. LipoylTrfase_LipoateP_Ligase. [Graphical view]
Pfam	PF03099. BPL_LipA_LipB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00545. lipoyltrans. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LPLA_YEAST

Accession

Primary (citable) accession number: P47051

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	June 16, 2009
Last modified:	June 16, 2009
This is version 56 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)