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Protein

Mitochondrial import inner membrane translocase subunit TIM54

Gene

TIM54

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Essential component of the TIM22 complex, a complex that mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. The TIM22 complex forms a twin-pore translocase that uses the membrane potential as external driving force. Its precise function within the TIM22 complex is unclear.3 Publications

GO - Biological processi

  • protein import into mitochondrial inner membrane Source: SGD
  • protein targeting to mitochondrion Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31517-MONOMER.
ReactomeiR-SCE-1268020. Mitochondrial protein import.

Protein family/group databases

TCDBi3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial import inner membrane translocase subunit TIM54
Gene namesi
Name:TIM54
Ordered Locus Names:YJL054W
ORF Names:J1150
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJL054W.
SGDiS000003590. TIM54.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 32Mitochondrial matrixSequence analysisAdd BLAST32
Transmembranei33 – 49HelicalSequence analysisAdd BLAST17
Topological domaini50 – 478Mitochondrial intermembraneSequence analysisAdd BLAST429

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002030621 – 478Mitochondrial import inner membrane translocase subunit TIM54Add BLAST478

Proteomic databases

MaxQBiP47045.
PRIDEiP47045.

PTM databases

iPTMnetiP47045.

Interactioni

Subunit structurei

Component of the TIM22 complex, whose core is composed of TIM18, TIM22 and TIM54, associated with the peripheral proteins MRS5/TIM12 and the 70 kDa heterohexamer composed of TIM9 and TIM10 (or TIM8 and TIM13).3 Publications

Protein-protein interaction databases

BioGridi33700. 13 interactors.
DIPiDIP-5898N.
IntActiP47045. 4 interactors.
MINTiMINT-500264.

Structurei

3D structure databases

ProteinModelPortaliP47045.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TIM54 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000248043.
InParanoidiP47045.
KOiK17792.
OMAiFVQGRQQ.
OrthoDBiEOG092C40WX.

Family and domain databases

InterProiIPR021056. Mt_import_IM_translocase_Tim54.
[Graphical view]
PfamiPF11711. Tim54. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47045-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSESGKPIA KPIRKPGYTN PALKALGIPA LRLPSRNWMI FWSVLTVSIG
60 70 80 90 100
GIAYDKYKQR QILSHATDLV KPLAEESMEV DKVPRKITVF IAPPPNDYLE
110 120 130 140 150
SSLKVWRRYV KPVLYYAGLD YELVQEDRQG IIRTNVANRI RELRKEILAS
160 170 180 190 200
TDGQPVKEPN QTVAKPSGSS TSKISSLLPF NKIIQDPAEE DDSFDPEIGK
210 220 230 240 250
KFKENFDWRN VIGIFYTMPK PKHIISEDAL TKDPILSGGV ICLGRGAYKE
260 270 280 290 300
YIAGIHEGLL GPIEKTEKTG STEPKMTGVV EANQIESKVS ESGATELVDA
310 320 330 340 350
EKETALEEAK VQDDLKVDEE NSSEDSQKFL KPFISSDQYP DLQIASELQT
360 370 380 390 400
PNGEFIRNPN TNIPLLINQP LLVIPIPNLI GFTTIPRRIH RFYQKRFYVE
410 420 430 440 450
DVCSSVVNCV RQTRIRPFDI AKDIDLAKDE EKDWPQNWVK QGKEKNSEWT
460 470
QELVCDPRIT KHMFVYEKPP KEEPESDI
Length:478
Mass (Da):54,183
Last modified:February 1, 1996 - v1
Checksum:i0E682BB4035E3A4C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49329 Genomic DNA. Translation: CAA89345.1.
BK006943 Genomic DNA. Translation: DAA08744.1.
PIRiS56826.
RefSeqiNP_012481.3. NM_001181487.3.

Genome annotation databases

EnsemblFungiiYJL054W; YJL054W; YJL054W.
GeneIDi853392.
KEGGisce:YJL054W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49329 Genomic DNA. Translation: CAA89345.1.
BK006943 Genomic DNA. Translation: DAA08744.1.
PIRiS56826.
RefSeqiNP_012481.3. NM_001181487.3.

3D structure databases

ProteinModelPortaliP47045.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33700. 13 interactors.
DIPiDIP-5898N.
IntActiP47045. 4 interactors.
MINTiMINT-500264.

Protein family/group databases

TCDBi3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

PTM databases

iPTMnetiP47045.

Proteomic databases

MaxQBiP47045.
PRIDEiP47045.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJL054W; YJL054W; YJL054W.
GeneIDi853392.
KEGGisce:YJL054W.

Organism-specific databases

EuPathDBiFungiDB:YJL054W.
SGDiS000003590. TIM54.

Phylogenomic databases

HOGENOMiHOG000248043.
InParanoidiP47045.
KOiK17792.
OMAiFVQGRQQ.
OrthoDBiEOG092C40WX.

Enzyme and pathway databases

BioCyciYEAST:G3O-31517-MONOMER.
ReactomeiR-SCE-1268020. Mitochondrial protein import.

Miscellaneous databases

PROiP47045.

Family and domain databases

InterProiIPR021056. Mt_import_IM_translocase_Tim54.
[Graphical view]
PfamiPF11711. Tim54. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTIM54_YEAST
AccessioniPrimary (citable) accession number: P47045
Secondary accession number(s): D6VWC8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 2, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.