P47045 (TIM54_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitochondrial import inner membrane translocase subunit TIM54

Gene names

Name:	TIM54
Ordered Locus Names:	YJL054W
ORF Names:	J1150

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	478 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential component of the TIM22 complex, a complex that mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. The TIM22 complex forms a twin-pore translocase that uses the membrane potential as external driving force. Its precise function within the TIM22 complex is unclear. Ref.3 Ref.5 Ref.7
Subunit structure	Component of the TIM22 complex, whose core is composed of TIM18, TIM22 and TIM54, associated with the peripheral proteins MRS5/TIM12 and the 70 kDa heterohexamer composed of TIM9 and TIM10 (or TIM8 and TIM13).
Subcellular location	Mitochondrion inner membrane; Single-pass membrane protein. Note: Import into inner membrane protein requires TOM70 function. Ref.3 Ref.4 Ref.5 Ref.8
Sequence similarities	Belongs to the TIM54 family.

Ontologies

Keywords
Biological process	Protein transport Translocation Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein import into mitochondrial inner membrane Inferred from mutant phenotype Ref.3. Source: SGD
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial inner membrane protein insertion complex Inferred from direct assay Ref.5Ref.6. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 478	478	Mitochondrial import inner membrane translocase subunit TIM54		PRO_0000203062
Regions
	Topological domain	1 – 32	32	Mitochondrial matrix Potential
	Transmembrane	33 – 49	17	Helical; Potential
	Topological domain	50 – 478	429	Mitochondrial intermembrane Potential

Sequences

	Sequence		Length	Mass (Da)
	P47045 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 0E682BB4035E3A4C	FASTA	478	54,183
	10 20 30 40 50 60 MSSESGKPIA KPIRKPGYTN PALKALGIPA LRLPSRNWMI FWSVLTVSIG GIAYDKYKQR 70 80 90 100 110 120 QILSHATDLV KPLAEESMEV DKVPRKITVF IAPPPNDYLE SSLKVWRRYV KPVLYYAGLD 130 140 150 160 170 180 YELVQEDRQG IIRTNVANRI RELRKEILAS TDGQPVKEPN QTVAKPSGSS TSKISSLLPF 190 200 210 220 230 240 NKIIQDPAEE DDSFDPEIGK KFKENFDWRN VIGIFYTMPK PKHIISEDAL TKDPILSGGV 250 260 270 280 290 300 ICLGRGAYKE YIAGIHEGLL GPIEKTEKTG STEPKMTGVV EANQIESKVS ESGATELVDA 310 320 330 340 350 360 EKETALEEAK VQDDLKVDEE NSSEDSQKFL KPFISSDQYP DLQIASELQT PNGEFIRNPN 370 380 390 400 410 420 TNIPLLINQP LLVIPIPNLI GFTTIPRRIH RFYQKRFYVE DVCSSVVNCV RQTRIRPFDI 430 440 450 460 470 AKDIDLAKDE EKDWPQNWVK QGKEKNSEWT QELVCDPRIT KHMFVYEKPP KEEPESDI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"The Tim54p-Tim22p complex mediates insertion of proteins into the mitochondrial inner membrane." Kerscher O., Holder J., Srinivasan M., Leung R.S., Jensen R.E. J. Cell Biol. 139:1663-1675(1997) [PubMed: 9412462] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY, INTERACTION WITH TIM22.
[4]	"Biogenesis of Tim proteins of the mitochondrial carrier import pathway: differential targeting mechanisms and crossing over with the main import pathway." Kurz M., Martin H., Rassow J., Pfanner N., Ryan M.T. Mol. Biol. Cell 10:2461-2474(1999) [PubMed: 10397776] [Abstract] Cited for: SUBCELLULAR LOCATION.
[5]	"Tim18p is a new component of the Tim54p-Tim22p translocon in the mitochondrial inner membrane." Kerscher O., Sepuri N.B., Jensen R.E. Mol. Biol. Cell 11:103-116(2000) [PubMed: 10637294] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY, IDENTIFICATION IN THE TIM22 COMPLEX WITH TIM22 AND TIM18.
[6]	"Tim18p, a new subunit of the TIM22 complex that mediates insertion of imported proteins into the yeast mitochondrial inner membrane." Koehler C.M., Murphy M.P., Bally N.A., Leuenberger D., Oppliger W., Dolfini L., Junne T., Schatz G., Or E. Mol. Cell. Biol. 20:1187-1193(2000) [PubMed: 10648604] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE TIM22 COMPLEX WITH TIM12; TIM22 AND TIM18.
[7]	"Tim22, the essential core of the mitochondrial protein insertion complex, forms a voltage-activated and signal-gated channel." Kovermann P., Truscott K.N., Guiard B., Rehling P., Sepuri N.B., Mueller H., Jensen R.E., Wagner R., Pfanner N. Mol. Cell 9:363-373(2002) [PubMed: 11864609] [Abstract] Cited for: FUNCTION.
[8]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]	"Protein insertion into the mitochondrial inner membrane by a twin-pore translocase." Rehling P., Model K., Brandner K., Kovermann P., Sickmann A., Meyer H.E., Kuehlbrandt W., Wagner R., Truscott K.N., Pfanner N. Science 299:1747-1751(2003) [PubMed: 12637749] [Abstract] Cited for: IDENTIFICATION IN THE TIM22 COMPLEX WITH TIM10; TIM12; TIM22 AND TIM54.
[10]	Erratum Rehling P., Model K., Brandner K., Kovermann P., Sickmann A., Meyer H.E., Kuehlbrandt W., Wagner R., Truscott K.N., Pfanner N. Science 300:251-251(2003)
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z49329 Genomic DNA. Translation: CAA89345.1. BK006943 Genomic DNA. Translation: DAA08744.1.
PIR	S56826.
RefSeq	NP_012481.1. NM_001181487.1.
3D structure databases
ProteinModelPortal	P47045.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5898N.
IntAct	P47045. 4 interactions.
MINT	MINT-500264.
STRING	P47045.
Protein family/group databases
TCDB	3.A.8.1.1. mitochondrial protein translocase (MPT) family.
Proteomic databases
PeptideAtlas	P47045.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJL054W; YJL054W; YJL054W.
GeneID	853392.
KEGG	sce:YJL054W.
NMPDR	fig|4932.3.peg.3453.
Organism-specific databases
CYGD	YJL054w.
SGD	S000003590. TIM54.
Phylogenomic databases
eggNOG	fuNOG04778.
GeneTree	EFGT00050000006127.
HOGENOM	HBG397749.
OMA	VIGRHTW.
OrthoDB	EOG4KM2C5.
Gene expression databases
ArrayExpress	P47045.
Genevestigator	P47045.
GermOnline	YJL054W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR021056. Mt_import_IM_translocase_Tim54. [Graphical view]
Pfam	PF11711. Tim54. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	973867.

Entry information

Entry name

TIM54_YEAST

Accession

Primary (citable) accession number: P47045
Secondary accession number(s): D6VWC8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	December 14, 2011
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program