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P47041

- BIT61_YEAST

UniProt

P47041 - BIT61_YEAST

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Protein

Target of rapamycin complex 2 subunit BIT61

Gene

BIT61

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Component of TORC2, which regulates cell cycle-dependent polarization of the actin-cytoskeleton and cell wall integrity. TORC2 controls polarity of the actin cytoskeleton, which is required for orienting the secretory pathway toward discrete growth sites, via the RHO1/PKC1/MAPK cell integrity pathway.1 Publication

GO - Biological processi

  1. establishment or maintenance of actin cytoskeleton polarity Source: SGD
  2. fungal-type cell wall organization Source: SGD
  3. regulation of cell growth Source: SGD
  4. TOR signaling Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-31521-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Target of rapamycin complex 2 subunit BIT61
Short name:
TORC2 subunit BIT61
Alternative name(s):
61 kDa binding partner of TOR2 protein
Gene namesi
Name:BIT61
Ordered Locus Names:YJL058C
ORF Names:J1141
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

CYGDiYJL058c.
SGDiS000003594. BIT61.

Subcellular locationi

GO - Cellular componenti

  1. plasma membrane Source: SGD
  2. TORC2 complex Source: SGD
  3. vacuole Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 543543Target of rapamycin complex 2 subunit BIT61PRO_0000203060Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei58 – 581Phosphotyrosine1 Publication
Modified residuei59 – 591Phosphoserine1 Publication
Modified residuei139 – 1391Phosphoserine1 Publication
Modified residuei144 – 1441Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP47041.
PaxDbiP47041.

Expressioni

Gene expression databases

GenevestigatoriP47041.

Interactioni

Subunit structurei

The target of rapamycin complex 2 (TORC2) is composed of at least AVO1, AVO2, BIT61, LST8, TOR2 and TSC11. TORC2 likely forms a homodimer. Contrary to TORC1, TORC2 does not bind to and is not sensitive to FKBP-rapamycin.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
TOR2P326002EBI-25889,EBI-19385

Protein-protein interaction databases

BioGridi33696. 50 interactions.
DIPiDIP-1444N.
IntActiP47041. 18 interactions.
MINTiMINT-388996.
STRINGi4932.YJL058C.

Structurei

3D structure databases

ProteinModelPortaliP47041.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the BIT61 family.Curated

Phylogenomic databases

eggNOGiNOG44790.
GeneTreeiENSGT00390000008461.
HOGENOMiHOG000095231.
InParanoidiP47041.
OMAiNESRTHI.
OrthoDBiEOG7BCNMJ.

Family and domain databases

InterProiIPR013745. HbrB.
[Graphical view]
PfamiPF08539. HbrB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47041-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTAEDILLRE RTSTTTQRPV NSEQYLNVQL ATAPVKNFQT TSEISRQTLV
60 70 80 90 100
DTSNDDVYSI KNLKGSRNPI SPSVSNVGFQ SIFHTVDHPR SKVSVASNHS
110 120 130 140 150
LRSNDNASAA TSKSGSSQIG ESHSVDTVEC SNNLSKKLSS DAISITQKSL
160 170 180 190 200
HSTPSGRYMK GKASGFFNRR NRAHTTISSD PASFLTDSST LHNSSHSFRN
210 220 230 240 250
VIKNFFQNKS HRHIGQDAIE PAIPNSLSKF LHSSYGRHKS PSQFIHTNAG
260 270 280 290 300
QLVDSGTSVY SLNVNPSGVN PNTIVEDPLS GTDPASPNPV SMLHDLLRNL
310 320 330 340 350
PSLEANYKHF NSQELTTLTN NIWNIFCSNV AELFRTQRIW KLRAKIENFN
360 370 380 390 400
EVLEFYCILK TDPRVTHSGM NRIISDLKEF LVSSLYNLEN QIVFNYSNED
410 420 430 440 450
TINNALKRLG VIWRIFYQEV YYDLAAVLLP LDQSIREDGN STVLKSGNES
460 470 480 490 500
RTHINGNYSI GFLLLMCFRD SIVLPCYENF VNSNDGISKS FQLYIFNQEE
510 520 530 540
ESNVTETDKL TLLQCFGILS TIQSNDRNQR IIEELLAGIR MSI
Length:543
Mass (Da):60,841
Last modified:February 1, 1996 - v1
Checksum:i99F0F96840311F4B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49333 Genomic DNA. Translation: CAA89349.1.
AY693068 Genomic DNA. Translation: AAT93087.1.
BK006943 Genomic DNA. Translation: DAA08740.1.
PIRiS56830.
RefSeqiNP_012477.1. NM_001181491.1.

Genome annotation databases

EnsemblFungiiYJL058C; YJL058C; YJL058C.
GeneIDi853388.
KEGGisce:YJL058C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49333 Genomic DNA. Translation: CAA89349.1 .
AY693068 Genomic DNA. Translation: AAT93087.1 .
BK006943 Genomic DNA. Translation: DAA08740.1 .
PIRi S56830.
RefSeqi NP_012477.1. NM_001181491.1.

3D structure databases

ProteinModelPortali P47041.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33696. 50 interactions.
DIPi DIP-1444N.
IntActi P47041. 18 interactions.
MINTi MINT-388996.
STRINGi 4932.YJL058C.

Proteomic databases

MaxQBi P47041.
PaxDbi P47041.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YJL058C ; YJL058C ; YJL058C .
GeneIDi 853388.
KEGGi sce:YJL058C.

Organism-specific databases

CYGDi YJL058c.
SGDi S000003594. BIT61.

Phylogenomic databases

eggNOGi NOG44790.
GeneTreei ENSGT00390000008461.
HOGENOMi HOG000095231.
InParanoidi P47041.
OMAi NESRTHI.
OrthoDBi EOG7BCNMJ.

Enzyme and pathway databases

BioCyci YEAST:G3O-31521-MONOMER.

Miscellaneous databases

NextBioi 973855.

Gene expression databases

Genevestigatori P47041.

Family and domain databases

InterProi IPR013745. HbrB.
[Graphical view ]
Pfami PF08539. HbrB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "TOR complex 1 includes a novel component, Tco89p (YPL180w), and cooperates with Ssd1p to maintain cellular integrity in Saccharomyces cerevisiae."
    Reinke A., Anderson S., McCaffery J.M., Yates J.R. III, Aronova S., Chu S., Fairclough S., Iverson C., Wedaman K.P., Powers T.
    J. Biol. Chem. 279:14752-14762(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN TORC2, IDENTIFICATION BY MASS SPECTROMETRY.
  6. "The pleckstrin homology domain proteins Slm1 and Slm2 are required for actin cytoskeleton organization in yeast and bind phosphatidylinositol-4,5-bisphosphate and TORC2."
    Fadri M., Daquinag A., Wang S., Xue T., Kunz J.
    Mol. Biol. Cell 16:1883-1900(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SLM1 AND SLM2.
  7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-144, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-58; SER-59; SER-139 AND SER-144, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiBIT61_YEAST
AccessioniPrimary (citable) accession number: P47041
Secondary accession number(s): D6VWC4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: October 29, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1960 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3