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P47041

- BIT61_YEAST

UniProt

P47041 - BIT61_YEAST

Protein

Target of rapamycin complex 2 subunit BIT61

Gene

BIT61

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Component of TORC2, which regulates cell cycle-dependent polarization of the actin-cytoskeleton and cell wall integrity. TORC2 controls polarity of the actin cytoskeleton, which is required for orienting the secretory pathway toward discrete growth sites, via the RHO1/PKC1/MAPK cell integrity pathway.1 Publication

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. establishment or maintenance of actin cytoskeleton polarity Source: SGD
    2. fungal-type cell wall organization Source: SGD
    3. regulation of cell growth Source: SGD
    4. TOR signaling Source: SGD

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31521-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Target of rapamycin complex 2 subunit BIT61
    Short name:
    TORC2 subunit BIT61
    Alternative name(s):
    61 kDa binding partner of TOR2 protein
    Gene namesi
    Name:BIT61
    Ordered Locus Names:YJL058C
    ORF Names:J1141
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome X

    Organism-specific databases

    CYGDiYJL058c.
    SGDiS000003594. BIT61.

    Subcellular locationi

    GO - Cellular componenti

    1. plasma membrane Source: SGD
    2. TORC2 complex Source: SGD
    3. vacuolar membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane, Vacuole

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 543543Target of rapamycin complex 2 subunit BIT61PRO_0000203060Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei58 – 581Phosphotyrosine1 Publication
    Modified residuei59 – 591Phosphoserine1 Publication
    Modified residuei139 – 1391Phosphoserine1 Publication
    Modified residuei144 – 1441Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP47041.
    PaxDbiP47041.

    Expressioni

    Gene expression databases

    GenevestigatoriP47041.

    Interactioni

    Subunit structurei

    The target of rapamycin complex 2 (TORC2) is composed of at least AVO1, AVO2, BIT61, LST8, TOR2 and TSC11. TORC2 likely forms a homodimer. Contrary to TORC1, TORC2 does not bind to and is not sensitive to FKBP-rapamycin.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    TOR2P326002EBI-25889,EBI-19385

    Protein-protein interaction databases

    BioGridi33696. 50 interactions.
    DIPiDIP-1444N.
    IntActiP47041. 18 interactions.
    MINTiMINT-388996.
    STRINGi4932.YJL058C.

    Structurei

    3D structure databases

    ProteinModelPortaliP47041.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the BIT61 family.Curated

    Phylogenomic databases

    eggNOGiNOG44790.
    GeneTreeiENSGT00390000008461.
    HOGENOMiHOG000095231.
    OMAiNESRTHI.
    OrthoDBiEOG7BCNMJ.

    Family and domain databases

    InterProiIPR013745. HbrB.
    [Graphical view]
    PfamiPF08539. HbrB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P47041-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTAEDILLRE RTSTTTQRPV NSEQYLNVQL ATAPVKNFQT TSEISRQTLV    50
    DTSNDDVYSI KNLKGSRNPI SPSVSNVGFQ SIFHTVDHPR SKVSVASNHS 100
    LRSNDNASAA TSKSGSSQIG ESHSVDTVEC SNNLSKKLSS DAISITQKSL 150
    HSTPSGRYMK GKASGFFNRR NRAHTTISSD PASFLTDSST LHNSSHSFRN 200
    VIKNFFQNKS HRHIGQDAIE PAIPNSLSKF LHSSYGRHKS PSQFIHTNAG 250
    QLVDSGTSVY SLNVNPSGVN PNTIVEDPLS GTDPASPNPV SMLHDLLRNL 300
    PSLEANYKHF NSQELTTLTN NIWNIFCSNV AELFRTQRIW KLRAKIENFN 350
    EVLEFYCILK TDPRVTHSGM NRIISDLKEF LVSSLYNLEN QIVFNYSNED 400
    TINNALKRLG VIWRIFYQEV YYDLAAVLLP LDQSIREDGN STVLKSGNES 450
    RTHINGNYSI GFLLLMCFRD SIVLPCYENF VNSNDGISKS FQLYIFNQEE 500
    ESNVTETDKL TLLQCFGILS TIQSNDRNQR IIEELLAGIR MSI 543
    Length:543
    Mass (Da):60,841
    Last modified:February 1, 1996 - v1
    Checksum:i99F0F96840311F4B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49333 Genomic DNA. Translation: CAA89349.1.
    AY693068 Genomic DNA. Translation: AAT93087.1.
    BK006943 Genomic DNA. Translation: DAA08740.1.
    PIRiS56830.
    RefSeqiNP_012477.1. NM_001181491.1.

    Genome annotation databases

    EnsemblFungiiYJL058C; YJL058C; YJL058C.
    GeneIDi853388.
    KEGGisce:YJL058C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49333 Genomic DNA. Translation: CAA89349.1 .
    AY693068 Genomic DNA. Translation: AAT93087.1 .
    BK006943 Genomic DNA. Translation: DAA08740.1 .
    PIRi S56830.
    RefSeqi NP_012477.1. NM_001181491.1.

    3D structure databases

    ProteinModelPortali P47041.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33696. 50 interactions.
    DIPi DIP-1444N.
    IntActi P47041. 18 interactions.
    MINTi MINT-388996.
    STRINGi 4932.YJL058C.

    Proteomic databases

    MaxQBi P47041.
    PaxDbi P47041.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YJL058C ; YJL058C ; YJL058C .
    GeneIDi 853388.
    KEGGi sce:YJL058C.

    Organism-specific databases

    CYGDi YJL058c.
    SGDi S000003594. BIT61.

    Phylogenomic databases

    eggNOGi NOG44790.
    GeneTreei ENSGT00390000008461.
    HOGENOMi HOG000095231.
    OMAi NESRTHI.
    OrthoDBi EOG7BCNMJ.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31521-MONOMER.

    Miscellaneous databases

    NextBioi 973855.

    Gene expression databases

    Genevestigatori P47041.

    Family and domain databases

    InterProi IPR013745. HbrB.
    [Graphical view ]
    Pfami PF08539. HbrB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. "TOR complex 1 includes a novel component, Tco89p (YPL180w), and cooperates with Ssd1p to maintain cellular integrity in Saccharomyces cerevisiae."
      Reinke A., Anderson S., McCaffery J.M., Yates J.R. III, Aronova S., Chu S., Fairclough S., Iverson C., Wedaman K.P., Powers T.
      J. Biol. Chem. 279:14752-14762(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION IN TORC2, IDENTIFICATION BY MASS SPECTROMETRY.
    6. "The pleckstrin homology domain proteins Slm1 and Slm2 are required for actin cytoskeleton organization in yeast and bind phosphatidylinositol-4,5-bisphosphate and TORC2."
      Fadri M., Daquinag A., Wang S., Xue T., Kunz J.
      Mol. Biol. Cell 16:1883-1900(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SLM1 AND SLM2.
    7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-144, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-58; SER-59; SER-139 AND SER-144, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiBIT61_YEAST
    AccessioniPrimary (citable) accession number: P47041
    Secondary accession number(s): D6VWC4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 104 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1960 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3