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P47041 (BIT61_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Target of rapamycin complex 2 subunit BIT61

Short name=TORC2 subunit BIT61
Alternative name(s):
61 kDa binding partner of TOR2 protein
Gene names
Name:BIT61
Ordered Locus Names:YJL058C
ORF Names:J1141
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length543 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of TORC2, which regulates cell cycle-dependent polarization of the actin-cytoskeleton and cell wall integrity. TORC2 controls polarity of the actin cytoskeleton, which is required for orienting the secretory pathway toward discrete growth sites, via the RHO1/PKC1/MAPK cell integrity pathway. Ref.5

Subunit structure

The target of rapamycin complex 2 (TORC2) is composed of at least AVO1, AVO2, BIT61, LST8, TOR2 and TSC11. TORC2 likely forms a homodimer. Contrary to TORC1, TORC2 does not bind to and is not sensitive to FKBP-rapamycin. Ref.5

Subcellular location

Cell membrane; Peripheral membrane protein; Cytoplasmic side. Vacuole membrane; Peripheral membrane protein; Cytoplasmic side Probable.

Miscellaneous

Present with 1960 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the BIT61 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

TOR2P326002EBI-25889,EBI-19385

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 543543Target of rapamycin complex 2 subunit BIT61
PRO_0000203060

Amino acid modifications

Modified residue581Phosphotyrosine Ref.9
Modified residue591Phosphoserine Ref.9
Modified residue1391Phosphoserine Ref.9
Modified residue1441Phosphoserine Ref.7 Ref.9

Sequences

Sequence LengthMass (Da)Tools
P47041 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: 99F0F96840311F4B

FASTA54360,841
        10         20         30         40         50         60 
MTAEDILLRE RTSTTTQRPV NSEQYLNVQL ATAPVKNFQT TSEISRQTLV DTSNDDVYSI 

        70         80         90        100        110        120 
KNLKGSRNPI SPSVSNVGFQ SIFHTVDHPR SKVSVASNHS LRSNDNASAA TSKSGSSQIG 

       130        140        150        160        170        180 
ESHSVDTVEC SNNLSKKLSS DAISITQKSL HSTPSGRYMK GKASGFFNRR NRAHTTISSD 

       190        200        210        220        230        240 
PASFLTDSST LHNSSHSFRN VIKNFFQNKS HRHIGQDAIE PAIPNSLSKF LHSSYGRHKS 

       250        260        270        280        290        300 
PSQFIHTNAG QLVDSGTSVY SLNVNPSGVN PNTIVEDPLS GTDPASPNPV SMLHDLLRNL 

       310        320        330        340        350        360 
PSLEANYKHF NSQELTTLTN NIWNIFCSNV AELFRTQRIW KLRAKIENFN EVLEFYCILK 

       370        380        390        400        410        420 
TDPRVTHSGM NRIISDLKEF LVSSLYNLEN QIVFNYSNED TINNALKRLG VIWRIFYQEV 

       430        440        450        460        470        480 
YYDLAAVLLP LDQSIREDGN STVLKSGNES RTHINGNYSI GFLLLMCFRD SIVLPCYENF 

       490        500        510        520        530        540 
VNSNDGISKS FQLYIFNQEE ESNVTETDKL TLLQCFGILS TIQSNDRNQR IIEELLAGIR 


MSI 

« Hide

References

« Hide 'large scale' references
[1]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]"TOR complex 1 includes a novel component, Tco89p (YPL180w), and cooperates with Ssd1p to maintain cellular integrity in Saccharomyces cerevisiae."
Reinke A., Anderson S., McCaffery J.M., Yates J.R. III, Aronova S., Chu S., Fairclough S., Iverson C., Wedaman K.P., Powers T.
J. Biol. Chem. 279:14752-14762(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, IDENTIFICATION IN TORC2, IDENTIFICATION BY MASS SPECTROMETRY.
[6]"The pleckstrin homology domain proteins Slm1 and Slm2 are required for actin cytoskeleton organization in yeast and bind phosphatidylinositol-4,5-bisphosphate and TORC2."
Fadri M., Daquinag A., Wang S., Xue T., Kunz J.
Mol. Biol. Cell 16:1883-1900(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SLM1 AND SLM2.
[7]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-144, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[8]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-58; SER-59; SER-139 AND SER-144, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z49333 Genomic DNA. Translation: CAA89349.1.
AY693068 Genomic DNA. Translation: AAT93087.1.
BK006943 Genomic DNA. Translation: DAA08740.1.
PIRS56830.
RefSeqNP_012477.1. NM_001181491.1.

3D structure databases

ProteinModelPortalP47041.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33696. 50 interactions.
DIPDIP-1444N.
IntActP47041. 18 interactions.
MINTMINT-388996.
STRING4932.YJL058C.

Proteomic databases

PaxDbP47041.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJL058C; YJL058C; YJL058C.
GeneID853388.
KEGGsce:YJL058C.

Organism-specific databases

CYGDYJL058c.
SGDS000003594. BIT61.

Phylogenomic databases

eggNOGNOG44790.
GeneTreeENSGT00390000008461.
HOGENOMHOG000095231.
OrthoDBEOG7BCNMJ.

Enzyme and pathway databases

BioCycYEAST:G3O-31521-MONOMER.

Gene expression databases

GenevestigatorP47041.

Family and domain databases

InterProIPR013745. HbrB.
[Graphical view]
PfamPF08539. HbrB. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio973855.

Entry information

Entry nameBIT61_YEAST
AccessionPrimary (citable) accession number: P47041
Secondary accession number(s): D6VWC4
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: April 16, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families