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Protein

Protein BTN1

Gene

YHC3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Plays a role in vacuolar arginine transport. Involved in pH homeostasis. May be involved in ion homeostasis together with IST2. Not necessary for mitochondrial function or ATP synthase degradation.5 Publications

GO - Biological processi

  • arginine transport Source: SGD
  • lysine transport Source: SGD
  • regulation of intracellular pH Source: SGD
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31522-MONOMER.

Protein family/group databases

TCDBi2.A.57.5.2. the equilibrative nucleoside transporter (ent) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein BTN1
Gene namesi
Name:YHC3
Synonyms:BTN1
Ordered Locus Names:YJL059W
ORF Names:J1139
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJL059W.
SGDiS000003595. YHC3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei42 – 6221HelicalSequence analysisAdd
BLAST
Transmembranei80 – 10021HelicalSequence analysisAdd
BLAST
Transmembranei128 – 14821HelicalSequence analysisAdd
BLAST
Transmembranei150 – 17021HelicalSequence analysisAdd
BLAST
Transmembranei238 – 25821HelicalSequence analysisAdd
BLAST
Transmembranei323 – 34321HelicalSequence analysisAdd
BLAST
Transmembranei369 – 38921HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • fungal-type vacuole Source: SGD
  • integral component of membrane Source: UniProtKB-KW
  • vacuolar membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3030Sequence analysisAdd
BLAST
Chaini31 – 408378Protein BTN1PRO_0000020836Add
BLAST

Interactioni

Protein-protein interaction databases

BioGridi33695. 22 interactions.
DIPiDIP-4761N.
IntActiP47040. 1 interaction.
MINTiMINT-567364.

Structurei

3D structure databases

ProteinModelPortaliP47040.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the battenin family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000003249.
HOGENOMiHOG000164765.
InParanoidiP47040.
KOiK12389.
OMAiKHIEKPC.
OrthoDBiEOG7J44H9.

Family and domain databases

InterProiIPR003492. Battenin_disease_Cln3.
IPR018460. Battenin_disease_Cln3_subgr.
IPR020846. MFS_dom.
[Graphical view]
PANTHERiPTHR10981. PTHR10981. 1 hit.
PfamiPF02487. CLN3. 1 hit.
[Graphical view]
PIRSFiPIRSF015974. CLN3_BTN1. 1 hit.
PRINTSiPR01315. BATTENIN.
SUPFAMiSSF103473. SSF103473. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P47040-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDKSHQIYC YFWLFGLINN VLYVVILSAA VDIVGPTLPK SLVLLADIFP
60 70 80 90 100
SLAIKLCSPF FIDRIKYSYR IWSLITMSCL GMFLVSFKNL FVCLLGISFA
110 120 130 140 150
SISSGFGEVT FLQLTHYYKQ ISLNGWSSGT GGAGIIGGAS YMFLTSIFKV
160 170 180 190 200
PVKLTLLVFS LLPFAFLFYF KLESNDTNLT YQSLQQIDEA EDDQLVPFPV
210 220 230 240 250
AFTHTNASQS LYSTRQHILQ TVKRLRRLVF PYMVPLTTVY LFEYLINQAV
260 270 280 290 300
APTLLFPING DERSKSMPFF FHKYRDIYVT YGTLYQLGVF ISRSFGHLMR
310 320 330 340 350
MRSLYILAFL QGVNLCITVL QSWFYVTHSP WAVMILIFYE GFLGGASYVN
360 370 380 390 400
TFLNILEQED PDETEFAMGA VSIADSFGVF LAALLGLGLE PKLCRHQIAD

DRPWCRME
Length:408
Mass (Da):46,383
Last modified:February 1, 1996 - v1
Checksum:iBAB6891F676E80EE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti162 – 1621L → P in AAT93158 (PubMed:17322287).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti181 – 1811Y → F in strain: K289-3A. 1 Publication
Natural varianti328 – 3281H → R in strain: K289-3A. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF058447 Genomic DNA. Translation: AAC61258.1.
Z49334 Genomic DNA. Translation: CAA89350.1.
AY693139 Genomic DNA. Translation: AAT93158.1.
BK006943 Genomic DNA. Translation: DAA08739.1.
PIRiS56831.
RefSeqiNP_012476.1. NM_001181492.1.

Genome annotation databases

EnsemblFungiiYJL059W; YJL059W; YJL059W.
GeneIDi853387.
KEGGisce:YJL059W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF058447 Genomic DNA. Translation: AAC61258.1.
Z49334 Genomic DNA. Translation: CAA89350.1.
AY693139 Genomic DNA. Translation: AAT93158.1.
BK006943 Genomic DNA. Translation: DAA08739.1.
PIRiS56831.
RefSeqiNP_012476.1. NM_001181492.1.

3D structure databases

ProteinModelPortaliP47040.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33695. 22 interactions.
DIPiDIP-4761N.
IntActiP47040. 1 interaction.
MINTiMINT-567364.

Protein family/group databases

TCDBi2.A.57.5.2. the equilibrative nucleoside transporter (ent) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJL059W; YJL059W; YJL059W.
GeneIDi853387.
KEGGisce:YJL059W.

Organism-specific databases

EuPathDBiFungiDB:YJL059W.
SGDiS000003595. YHC3.

Phylogenomic databases

GeneTreeiENSGT00390000003249.
HOGENOMiHOG000164765.
InParanoidiP47040.
KOiK12389.
OMAiKHIEKPC.
OrthoDBiEOG7J44H9.

Enzyme and pathway databases

BioCyciYEAST:G3O-31522-MONOMER.

Miscellaneous databases

PROiP47040.

Family and domain databases

InterProiIPR003492. Battenin_disease_Cln3.
IPR018460. Battenin_disease_Cln3_subgr.
IPR020846. MFS_dom.
[Graphical view]
PANTHERiPTHR10981. PTHR10981. 1 hit.
PfamiPF02487. CLN3. 1 hit.
[Graphical view]
PIRSFiPIRSF015974. CLN3_BTN1. 1 hit.
PRINTSiPR01315. BATTENIN.
SUPFAMiSSF103473. SSF103473. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The subcellular location of the yeast Saccharomyces homologue of the protein defective in the juvenile form of Batten disease."
    Croopnick J.B., Choi H.C., Mueller D.M.
    Biochem. Biophys. Res. Commun. 250:335-341(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, VARIANTS PHE-181 AND ARG-328.
    Strain: K289-3A.
  2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "BTN1, a yeast gene corresponding to the human gene responsible for Batten's disease, is not essential for viability, mitochondrial function, or degradation of mitochondrial ATP synthase."
    Pearce D.A., Sherman F.
    Yeast 13:691-697(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Action of BTN1, the yeast orthologue of the gene mutated in Batten disease."
    Pearce D.A., Ferea T., Nosel S.A., Das B., Sherman F.
    Nat. Genet. 22:55-58(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  7. "The yeast model for Batten disease: mutations in BTN1, BTN2, and HSP30 alter pH homeostasis."
    Chattopadhyay S., Muzaffar N.E., Sherman F., Pearce D.A.
    J. Bacteriol. 182:6418-6423(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "A role in vacuolar arginine transport for yeast Btn1p and for human CLN3, the protein defective in Batten disease."
    Kim Y., Ramirez-Montealegre D., Pearce D.A.
    Proc. Natl. Acad. Sci. U.S.A. 100:15458-15462(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Interaction among Btn1p, Btn2p, and Ist2p reveals potential interplay among the vacuole, amino acid levels, and ion homeostasis in the yeast Saccharomyces cerevisiae."
    Kim Y., Chattopadhyay S., Locke S., Pearce D.A.
    Eukaryot. Cell 4:281-288(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiBTN1_YEAST
AccessioniPrimary (citable) accession number: P47040
Secondary accession number(s): D6VWC3, O60004, Q6B1E1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: June 8, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.