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P47037

- SMC3_YEAST

UniProt

P47037 - SMC3_YEAST

Protein

Structural maintenance of chromosomes protein 3

Gene

SMC3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 136 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi32 – 398ATPSequence Analysis

    GO - Molecular functioni

    1. ATPase activity Source: SGD
    2. ATP binding Source: UniProtKB-KW
    3. identical protein binding Source: IntAct
    4. protein binding Source: IntAct

    GO - Biological processi

    1. ascospore formation Source: SGD
    2. ATP catabolic process Source: GOC
    3. meiotic sister chromatid cohesion Source: SGD
    4. mitotic sister chromatid cohesion Source: SGD
    5. reciprocal meiotic recombination Source: SGD
    6. synaptonemal complex assembly Source: SGD

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31532-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Structural maintenance of chromosomes protein 3
    Alternative name(s):
    DA-box protein SMC3
    Gene namesi
    Name:SMC3
    Ordered Locus Names:YJL074C
    ORF Names:J1049
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome X

    Organism-specific databases

    CYGDiYJL074c.
    SGDiS000003610. SMC3.

    Subcellular locationi

    Nucleus. Chromosome
    Note: Associates with chromatin. Before prophase it is scattered along chromosome arms. At anaphase, the MCD1 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation.

    GO - Cellular componenti

    1. nuclear mitotic cohesin complex Source: SGD
    2. nucleus Source: SGD

    Keywords - Cellular componenti

    Chromosome, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12301230Structural maintenance of chromosomes protein 3PRO_0000119015Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei112 – 1121N6-acetyllysine1 Publication
    Modified residuei113 – 1131N6-acetyllysine1 Publication

    Post-translational modificationi

    Acetylation at Lys-112 and Lys-113 by ECO1 is important for genome stability and S phase sister chromatid cohesion.1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP47037.
    PaxDbiP47037.
    PeptideAtlasiP47037.

    Expressioni

    Gene expression databases

    GenevestigatoriP47037.

    Interactioni

    Subunit structurei

    Cohesin complexes are composed of the SMC1 and SMC3 heterodimer attached via their hinge domain, MCD1/SCC1 which link them, and IRR1/SCC3, which interacts with MCD1. The cohesin complex also interacts with SCC2, which is required for its association with chromosomes.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself5EBI-17423,EBI-17423
    IRR1P405412EBI-17423,EBI-16667
    MCD1Q1215813EBI-17423,EBI-16655
    RAD61Q993599EBI-17423,EBI-2082336
    SMC1P3290820EBI-17423,EBI-17402

    Protein-protein interaction databases

    BioGridi33681. 275 interactions.
    DIPiDIP-2991N.
    IntActiP47037. 55 interactions.
    MINTiMINT-435389.
    STRINGi4932.YJL074C.

    Structurei

    3D structure databases

    ProteinModelPortaliP47037.
    SMRiP47037. Positions 1-256, 504-694, 999-1218.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni483 – 684202Flexible hingeAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili172 – 482311Sequence AnalysisAdd
    BLAST
    Coiled coili685 – 1041357Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi1126 – 116136Ala/Asp-rich (DA-box)Add
    BLAST

    Domaini

    The flexible hinge domain, which separates the large intramolecular coiled coil regions, allows the heterotypic interaction with the corresponding domain of SMC1, forming a V-shaped heterodimer. The two heads of the heterodimer are then connected by different ends of the cleavable MCD1 protein, forming a ring structure By similarity.By similarity

    Sequence similaritiesi

    Belongs to the SMC family. SMC3 subfamily.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiCOG1196.
    GeneTreeiENSGT00580000081628.
    HOGENOMiHOG000166512.
    KOiK06669.
    OMAiKKERDEW.
    OrthoDBiEOG7M0P0T.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR027417. P-loop_NTPase.
    IPR003395. RecF/RecN/SMC_N.
    IPR010935. SMC_hinge.
    [Graphical view]
    PfamiPF06470. SMC_hinge. 1 hit.
    PF02463. SMC_N. 1 hit.
    [Graphical view]
    SMARTiSM00968. SMC_hinge. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 2 hits.
    SSF75553. SSF75553. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P47037-1 [UniParc]FASTAAdd to Basket

    « Hide

    MYIKRVIIKG FKTYRNETII DNFSPHQNVI IGSNGSGKSN FFAAIRFVLS     50
    DDYSNLKREE RQGLIHQGSG GSVMSASVEI VFHDPDHSMI LPSGVLSRGD 100
    DEVTIRRTVG LKKDDYQLND RNVTKGDIVR MLETAGFSMN NPYNIVPQGK 150
    IVALTNAKDK ERLQLLEDVV GAKSFEVKLK ASLKKMEETE QKKIQINKEM 200
    GELNSKLSEM EQERKELEKY NELERNRKIY QFTLYDRELN EVINQMERLD 250
    GDYNNTVYSS EQYIQELDKR EDMIDQVSKK LSSIEASLKI KNATDLQQAK 300
    LRESEISQKL TNVNVKIKDV QQQIESNEEQ RNLDSATLKE IKSIIEQRKQ 350
    KLSKILPRYQ ELTKEEAMYK LQLASLQQKQ RDLILKKGEY ARFKSKDERD 400
    TWIHSEIEEL KSSIQNLNEL ESQLQMDRTS LRKQYSAIDE EIEELIDSIN 450
    GPDTKGQLED FDSELIHLKQ KLSESLDTRK ELWRKEQKLQ TVLETLLSDV 500
    NQNQRNVNET MSRSLANGII NVKEITEKLK ISPESVFGTL GELIKVNDKY 550
    KTCAEVIGGN SLFHIVVDTE ETATLIMNEL YRMKGGRVTF IPLNRLSLDS 600
    DVKFPSNTTT QIQFTPLIKK IKYEPRFEKA VKHVFGKTIV VKDLGQGLKL 650
    AKKHKLNAIT LDGDRADKRG VLTGGYLDQH KRTRLESLKN LNESRSQHKK 700
    ILEELDFVRN ELNDIDTKID QVNGNIRKVS NDRESVLTNI EVYRTSLNTK 750
    KNEKLILEES LNAIILKLEK LNTNRTFAQE KLNTFENDLL QEFDSELSKE 800
    EKERLESLTK EISAAHNKLN ITSDALEGIT TTIDSLNAEL ESKLIPQEND 850
    LESKMSEVGD AFIFGLQDEL KELQLEKESV EKQHENAVLE LGTVQREIES 900
    LIAEETNNKK LLEKANNQQR LLLKKLDNFQ KSVEKTMIKK TTLVTRREEL 950
    QQRIREIGLL PEDALVNDFS DITSDQLLQR LNDMNTEISG LKNVNKRAFE 1000
    NFKKFNERRK DLAERASELD ESKDSIQDLI VKLKQQKVNA VDSTFQKVSE 1050
    NFEAVFERLV PRGTAKLIIH RKNDNANDHD ESIDVDMDAE SNESQNGKDS 1100
    EIMYTGVSIS VSFNSKQNEQ LHVEQLSGGQ KTVCAIALIL AIQMVDPASF 1150
    YLFDEIDAAL DKQYRTAVAT LLKELSKNAQ FICTTFRTDM LQVADKFFRV 1200
    KYENKISTVI EVNREEAIGF IRGSNKFAEV 1230
    Length:1,230
    Mass (Da):141,336
    Last modified:February 1, 1996 - v1
    Checksum:iB152D88F7780341F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y14278 Genomic DNA. Translation: CAA74655.1.
    Z49349 Genomic DNA. Translation: CAA89366.1.
    X88851 Genomic DNA. Translation: CAA61313.1.
    BK006943 Genomic DNA. Translation: DAA08725.1.
    PIRiS56850.
    RefSeqiNP_012461.1. NM_001181507.1.

    Genome annotation databases

    EnsemblFungiiYJL074C; YJL074C; YJL074C.
    GeneIDi853371.
    KEGGisce:YJL074C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y14278 Genomic DNA. Translation: CAA74655.1 .
    Z49349 Genomic DNA. Translation: CAA89366.1 .
    X88851 Genomic DNA. Translation: CAA61313.1 .
    BK006943 Genomic DNA. Translation: DAA08725.1 .
    PIRi S56850.
    RefSeqi NP_012461.1. NM_001181507.1.

    3D structure databases

    ProteinModelPortali P47037.
    SMRi P47037. Positions 1-256, 504-694, 999-1218.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33681. 275 interactions.
    DIPi DIP-2991N.
    IntActi P47037. 55 interactions.
    MINTi MINT-435389.
    STRINGi 4932.YJL074C.

    Proteomic databases

    MaxQBi P47037.
    PaxDbi P47037.
    PeptideAtlasi P47037.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YJL074C ; YJL074C ; YJL074C .
    GeneIDi 853371.
    KEGGi sce:YJL074C.

    Organism-specific databases

    CYGDi YJL074c.
    SGDi S000003610. SMC3.

    Phylogenomic databases

    eggNOGi COG1196.
    GeneTreei ENSGT00580000081628.
    HOGENOMi HOG000166512.
    KOi K06669.
    OMAi KKERDEW.
    OrthoDBi EOG7M0P0T.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31532-MONOMER.

    Miscellaneous databases

    NextBioi 973810.
    PROi P47037.

    Gene expression databases

    Genevestigatori P47037.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    InterProi IPR027417. P-loop_NTPase.
    IPR003395. RecF/RecN/SMC_N.
    IPR010935. SMC_hinge.
    [Graphical view ]
    Pfami PF06470. SMC_hinge. 1 hit.
    PF02463. SMC_N. 1 hit.
    [Graphical view ]
    SMARTi SM00968. SMC_hinge. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 2 hits.
    SSF75553. SSF75553. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cohesins: chromosomal proteins that prevent premature separation of sister chromatids."
      Michaelis C., Ciosk R., Nasmyth K.
      Cell 91:35-45(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 200060 / W303.
    2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Sor F.J.
      Submitted (JUN-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: S288c / FY1678.
    5. "Yeast cohesin complex requires a conserved protein, Eco1p(Ctf7), to establish cohesion between sister chromatids during DNA replication."
      Toth A., Ciosk R., Uhlmann F., Galova M., Schleiffer A., Nasmyth K.
      Genes Dev. 13:320-333(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN A COHESIN COMPLEX WITH SMC1; IRR1 AND MCD1, INTERACTION OF THE COHESIN COMPLEX WITH SCC2.
    6. "Molecular architecture of SMC proteins and the yeast cohesin complex."
      Haering C.H., Loewe J., Hochwagen A., Nasmyth K.
      Mol. Cell 9:773-788(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN A COHESIN COMPLEX WITH SMC3; MCD1 AND IRR1, STRUCTURE.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Acetylation of Smc3 by Eco1 is required for S phase sister chromatid cohesion in both human and yeast."
      Zhang J., Shi X., Li Y., Kim B.J., Jia J., Huang Z., Yang T., Fu X., Jung S.Y., Wang Y., Zhang P., Kim S.T., Pan X., Qin J.
      Mol. Cell 31:143-151(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION AT LYS-112 AND LYS-113.

    Entry informationi

    Entry nameiSMC3_YEAST
    AccessioniPrimary (citable) accession number: P47037
    Secondary accession number(s): D6VWA9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 136 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2660 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3