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Protein

Nucleolar protein NET1

Gene

NET1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has a role in chromosome maintenance and is involved in mitotic exit. Inhibits the action of CDC14 by sequestering it in the nucleolus. Also binds to RNA polymerase I and stimulates rRNA synthesis. Influences RDNA chromatin by tethering SIR2 to rDNA in the nucleolus.2 Publications

Miscellaneous

Present with 1590 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • phosphatase activator activity Source: GO_Central
  • rDNA binding Source: SGD

GO - Biological processi

  • chromatin silencing at rDNA Source: SGD
  • negative regulation of septation initiation signaling Source: GO_Central
  • nucleolus organization Source: SGD
  • regulation of exit from mitosis Source: SGD

Keywordsi

Biological processCell cycle, Cell division, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-31533-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleolar protein NET1
Gene namesi
Name:NET1
Synonyms:CFI1, ESC5, SRM8
Ordered Locus Names:YJL076W
ORF Names:J1038
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJL076W.
SGDiS000003612. NET1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000967841 – 1189Nucleolar protein NET1Add BLAST1189

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei60PhosphoserineCombined sources1
Modified residuei166PhosphoserineCombined sources1
Modified residuei231PhosphoserineCombined sources1
Modified residuei252PhosphoserineCombined sources1
Modified residuei437PhosphoserineCombined sources1
Modified residuei439PhosphoserineCombined sources1
Modified residuei447PhosphoserineCombined sources1
Modified residuei452PhosphoserineCombined sources1
Modified residuei497PhosphoserineCombined sources1
Modified residuei676PhosphothreonineCombined sources1
Modified residuei830PhosphoserineCombined sources1
Modified residuei1042PhosphothreonineCombined sources1
Modified residuei1056PhosphoserineCombined sources1
Modified residuei1059PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by CDC5.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP47035.
PRIDEiP47035.

PTM databases

iPTMnetiP47035.

Interactioni

Subunit structurei

Component of the RENT complex which is composed of at least NET1, CDC14 and SIR2 (PubMed:12056824, PubMed:11511359). Interacts with NSI1 (PubMed:22362748).3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi33680. 93 interactors.
DIPiDIP-5153N.
IntActiP47035. 112 interactors.
MINTiMINT-500408.
STRINGi4932.YJL076W.

Structurei

3D structure databases

ProteinModelPortaliP47035.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi192 – 199Poly-Thr8
Compositional biasi328 – 335Poly-Ser8
Compositional biasi415 – 418Poly-Ser4
Compositional biasi982 – 988Poly-Ser7
Compositional biasi993 – 1000Poly-Ser8
Compositional biasi1171 – 1174Poly-Lys4

Sequence similaritiesi

To yeast YKR010c.Curated

Phylogenomic databases

GeneTreeiENSGT00620000088873.
InParanoidiP47035.
KOiK06687.
OMAiFQKSELL.
OrthoDBiEOG092C2FCX.

Family and domain databases

InterProiView protein in InterPro
IPR018844. Dnt1-like_N.
PfamiView protein in Pfam
PF10407. Cytokin_check_N. 1 hit.

Sequencei

Sequence statusi: Complete.

P47035-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYKLQVVLVP PSLQATMPIQ FGYGPTIAES SQLLPNRTNM AQSAGDASLQ
60 70 80 90 100
YANLRSANVS FTPSYFNQSR FRKFLLFTKP TNTLLNLSDE IIDKCEKMYP
110 120 130 140 150
SLQEDIEILS LQDNSGCDLD PDFLVKDVFN VNNIVRVILK NEIDLDDSAP
160 170 180 190 200
VSLYKSVKRS KLNNGSPQSV QPQQQIPSSS GVLRIAKKRP PTGTTTTTTI
210 220 230 240 250
RSATNGSMRV STPLARQIYP PPSSKIVSNN SDDEDEDIGE RSFLPPPTQP
260 270 280 290 300
QSPPIRISSG IDAGKKIKSS IVEEDIVSRS ATVDPDKTKQ QRLLSGTPIM
310 320 330 340 350
STMTPNRVTL TGQRVVSEHA HKNELVFSAS ASSSSFANGG TAAVTAQDIN
360 370 380 390 400
RKPPVTTPRI TSGMLKIPEP RISEIEKELK EGPSSPASIL PAKAAKIPMK
410 420 430 440 450
KPYLENGENY ESDDSSSSEN QETPETEPHS KASLQRSQSS IADNNGSPVK
460 470 480 490 500
NSPLGDAMPH NVHLAELPKA SNTSITKSSN GESWGKQQEH QPPRKSSLET
510 520 530 540 550
IVEKKSQAEP SGIVEPKRMT NFLDDNQVRE KEDTNDKLLE KEILPTIPHN
560 570 580 590 600
DQPILASSDK SNGTLKSLAG KVSSNNNASK EDGTIINGTI EDDGNDNDEV
610 620 630 640 650
DTTVRIVPQD SDSSSFPKSD LFKMIEGDDT DLPQWFKGKN SRTSGNSKNS
660 670 680 690 700
KPYTTVLNKD IDNSKPDPRN ILPQRTPRSA AKRAAQLLAG AKKNEVPQKS
710 720 730 740 750
TEDSSSAAST DDESESGIET DFSSDDDFKR KNMSVPNNGP KDISLHSLKG
760 770 780 790 800
SVVPVKDSKI INKEVDEERN DKRDSQKKSA VSESSVTNSK ISEQMAKSFY
810 820 830 840 850
PNSNKKQNEA TKVETKPATQ ASSFPVVGGS PSVATKGTTS FNEEGNRKNV
860 870 880 890 900
KTKAKNESAQ IDRQQKETTS RVADLKSANI GGEDLNKKAE GSKEPEKASA
910 920 930 940 950
NIQDANDKNN SKEKEDSKSK QVSQKKLKMT DHLKEGNVQL PKPSANDKLK
960 970 980 990 1000
DLKAKFTNSK TLVPPGIISN EKNNSSANDD DSSSSGSSTE DESSSSSSSS
1010 1020 1030 1040 1050
DEETSTSRKA RRVVVNTPRE PVRSSSKIEA PSPSVNKKIN ATPDKIPVTQ
1060 1070 1080 1090 1100
LMDMSSPPSV KSKTTSNPSS ILHDLPRKVR PSLSSLSDLV SRGIPDVKEK
1110 1120 1130 1140 1150
TSKSNEKSQT KAPSSSDDES SSDSDSNSSS DSVSDSSSDS KSESDSDDSG
1160 1170 1180
DSSDDGKSFI SAKSASAALG KKKKPSGGFA SLIKDFKKK
Length:1,189
Mass (Da):128,531
Last modified:February 1, 1996 - v1
Checksum:i80EDF9A8065EB5A2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49351 Genomic DNA. Translation: CAA89367.1.
BK006943 Genomic DNA. Translation: DAA08724.1.
PIRiS56852.
RefSeqiNP_012459.1. NM_001181509.1.

Genome annotation databases

EnsemblFungiiYJL076W; YJL076W; YJL076W.
GeneIDi853369.
KEGGisce:YJL076W.

Similar proteinsi

Entry informationi

Entry nameiNET1_YEAST
AccessioniPrimary (citable) accession number: P47035
Secondary accession number(s): D6VWA8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: September 27, 2017
This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names