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P47035

- NET1_YEAST

UniProt

P47035 - NET1_YEAST

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Protein
Nucleolar protein NET1
Gene
NET1, CFI1, ESC5, SRM8, YJL076W, J1038
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Has a role in chromosome maintenance and is involved in mitotic exit. Inhibits the action of CDC14 by sequestering it in the nucleolus. Also binds to RNA polymerase I and stimulates rRNA synthesis. Influences RDNA chromatin by tethering SIR2 to rDNA in the nucleolus.2 Publications

GO - Molecular functioni

  1. protein binding Source: IntAct
  2. rDNA binding Source: SGD

GO - Biological processi

  1. chromatin silencing at rDNA Source: SGD
  2. mitotic nuclear division Source: UniProtKB-KW
  3. nucleolus organization Source: SGD
  4. regulation of exit from mitosis Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-31533-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleolar protein NET1
Gene namesi
Name:NET1
Synonyms:CFI1, ESC5, SRM8
Ordered Locus Names:YJL076W
ORF Names:J1038
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

CYGDiYJL076w.
SGDiS000003612. NET1.

Subcellular locationi

Nucleusnucleolus 1 Publication

GO - Cellular componenti

  1. RENT complex Source: SGD
  2. nucleolus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11891189Nucleolar protein NET1
PRO_0000096784Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei60 – 601Phosphoserine1 Publication
Modified residuei166 – 1661Phosphoserine1 Publication
Modified residuei231 – 2311Phosphoserine3 Publications
Modified residuei252 – 2521Phosphoserine1 Publication
Modified residuei437 – 4371Phosphoserine1 Publication
Modified residuei439 – 4391Phosphoserine1 Publication
Modified residuei447 – 4471Phosphoserine1 Publication
Modified residuei452 – 4521Phosphoserine1 Publication
Modified residuei497 – 4971Phosphoserine2 Publications
Modified residuei676 – 6761Phosphothreonine1 Publication
Modified residuei830 – 8301Phosphoserine1 Publication
Modified residuei1042 – 10421Phosphothreonine2 Publications
Modified residuei1056 – 10561Phosphoserine1 Publication
Modified residuei1059 – 10591Phosphoserine1 Publication

Post-translational modificationi

Phosphorylated by CDC5.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP47035.
PaxDbiP47035.
PeptideAtlasiP47035.

Expressioni

Gene expression databases

GenevestigatoriP47035.

Interactioni

Subunit structurei

Component of the RENT complex which is composed of at least NET1, CDC14 and SIR2.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CDC14Q0068413EBI-25953,EBI-4192
CDC8P005722EBI-25953,EBI-9957

Protein-protein interaction databases

BioGridi33680. 85 interactions.
DIPiDIP-5153N.
IntActiP47035. 95 interactions.
MINTiMINT-500408.
STRINGi4932.YJL076W.

Structurei

3D structure databases

ProteinModelPortaliP47035.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi192 – 1998Poly-Thr
Compositional biasi328 – 3358Poly-Ser
Compositional biasi415 – 4184Poly-Ser
Compositional biasi982 – 9887Poly-Ser
Compositional biasi993 – 10008Poly-Ser
Compositional biasi1171 – 11744Poly-Lys

Sequence similaritiesi

To yeast YKR010c.

Phylogenomic databases

eggNOGiNOG12793.
GeneTreeiENSGT00620000088873.
KOiK06687.
OMAiQSPPIRI.
OrthoDBiEOG7DC2DV.

Family and domain databases

InterProiIPR018844. Dnt1-like_N.
[Graphical view]
PfamiPF10407. Cytokin_check_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47035-1 [UniParc]FASTAAdd to Basket

« Hide

MYKLQVVLVP PSLQATMPIQ FGYGPTIAES SQLLPNRTNM AQSAGDASLQ     50
YANLRSANVS FTPSYFNQSR FRKFLLFTKP TNTLLNLSDE IIDKCEKMYP 100
SLQEDIEILS LQDNSGCDLD PDFLVKDVFN VNNIVRVILK NEIDLDDSAP 150
VSLYKSVKRS KLNNGSPQSV QPQQQIPSSS GVLRIAKKRP PTGTTTTTTI 200
RSATNGSMRV STPLARQIYP PPSSKIVSNN SDDEDEDIGE RSFLPPPTQP 250
QSPPIRISSG IDAGKKIKSS IVEEDIVSRS ATVDPDKTKQ QRLLSGTPIM 300
STMTPNRVTL TGQRVVSEHA HKNELVFSAS ASSSSFANGG TAAVTAQDIN 350
RKPPVTTPRI TSGMLKIPEP RISEIEKELK EGPSSPASIL PAKAAKIPMK 400
KPYLENGENY ESDDSSSSEN QETPETEPHS KASLQRSQSS IADNNGSPVK 450
NSPLGDAMPH NVHLAELPKA SNTSITKSSN GESWGKQQEH QPPRKSSLET 500
IVEKKSQAEP SGIVEPKRMT NFLDDNQVRE KEDTNDKLLE KEILPTIPHN 550
DQPILASSDK SNGTLKSLAG KVSSNNNASK EDGTIINGTI EDDGNDNDEV 600
DTTVRIVPQD SDSSSFPKSD LFKMIEGDDT DLPQWFKGKN SRTSGNSKNS 650
KPYTTVLNKD IDNSKPDPRN ILPQRTPRSA AKRAAQLLAG AKKNEVPQKS 700
TEDSSSAAST DDESESGIET DFSSDDDFKR KNMSVPNNGP KDISLHSLKG 750
SVVPVKDSKI INKEVDEERN DKRDSQKKSA VSESSVTNSK ISEQMAKSFY 800
PNSNKKQNEA TKVETKPATQ ASSFPVVGGS PSVATKGTTS FNEEGNRKNV 850
KTKAKNESAQ IDRQQKETTS RVADLKSANI GGEDLNKKAE GSKEPEKASA 900
NIQDANDKNN SKEKEDSKSK QVSQKKLKMT DHLKEGNVQL PKPSANDKLK 950
DLKAKFTNSK TLVPPGIISN EKNNSSANDD DSSSSGSSTE DESSSSSSSS 1000
DEETSTSRKA RRVVVNTPRE PVRSSSKIEA PSPSVNKKIN ATPDKIPVTQ 1050
LMDMSSPPSV KSKTTSNPSS ILHDLPRKVR PSLSSLSDLV SRGIPDVKEK 1100
TSKSNEKSQT KAPSSSDDES SSDSDSNSSS DSVSDSSSDS KSESDSDDSG 1150
DSSDDGKSFI SAKSASAALG KKKKPSGGFA SLIKDFKKK 1189
Length:1,189
Mass (Da):128,531
Last modified:February 1, 1996 - v1
Checksum:i80EDF9A8065EB5A2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z49351 Genomic DNA. Translation: CAA89367.1.
BK006943 Genomic DNA. Translation: DAA08724.1.
PIRiS56852.
RefSeqiNP_012459.1. NM_001181509.1.

Genome annotation databases

EnsemblFungiiYJL076W; YJL076W; YJL076W.
GeneIDi853369.
KEGGisce:YJL076W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z49351 Genomic DNA. Translation: CAA89367.1 .
BK006943 Genomic DNA. Translation: DAA08724.1 .
PIRi S56852.
RefSeqi NP_012459.1. NM_001181509.1.

3D structure databases

ProteinModelPortali P47035.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33680. 85 interactions.
DIPi DIP-5153N.
IntActi P47035. 95 interactions.
MINTi MINT-500408.
STRINGi 4932.YJL076W.

Proteomic databases

MaxQBi P47035.
PaxDbi P47035.
PeptideAtlasi P47035.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YJL076W ; YJL076W ; YJL076W .
GeneIDi 853369.
KEGGi sce:YJL076W.

Organism-specific databases

CYGDi YJL076w.
SGDi S000003612. NET1.

Phylogenomic databases

eggNOGi NOG12793.
GeneTreei ENSGT00620000088873.
KOi K06687.
OMAi QSPPIRI.
OrthoDBi EOG7DC2DV.

Enzyme and pathway databases

BioCyci YEAST:G3O-31533-MONOMER.

Miscellaneous databases

NextBioi 973807.

Gene expression databases

Genevestigatori P47035.

Family and domain databases

InterProi IPR018844. Dnt1-like_N.
[Graphical view ]
Pfami PF10407. Cytokin_check_N. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Budding yeast Cdc5 phosphorylates Net1 and assists Cdc14 release from the nucleolus."
    Yoshida S., Toh-e A.
    Biochem. Biophys. Res. Commun. 294:687-691(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, PHOSPHORYLATION.
  4. "Net1 stimulates RNA polymerase I transcription and regulates nucleolar structure independently of controlling mitotic exit."
    Shou W., Sakamoto K.M., Keener J., Morimoto K.W., Traverso E.E., Azzam R., Hoppe G.J., Feldman R.M.R., DeModena J., Moazed D., Charbonneau H., Nomura M., Deshaies R.J.
    Mol. Cell 8:45-55(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-231; SER-497 AND THR-676, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  7. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-231; SER-252; SER-830 AND THR-1042, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-60; SER-166; SER-231; SER-437; SER-439; SER-447; SER-452; SER-497; THR-1042; SER-1056 AND SER-1059, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNET1_YEAST
AccessioniPrimary (citable) accession number: P47035
Secondary accession number(s): D6VWA8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: September 3, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1590 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3

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