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P47035

- NET1_YEAST

UniProt

P47035 - NET1_YEAST

Protein

Nucleolar protein NET1

Gene

NET1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Has a role in chromosome maintenance and is involved in mitotic exit. Inhibits the action of CDC14 by sequestering it in the nucleolus. Also binds to RNA polymerase I and stimulates rRNA synthesis. Influences RDNA chromatin by tethering SIR2 to rDNA in the nucleolus.2 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. rDNA binding Source: SGD

    GO - Biological processi

    1. chromatin silencing at rDNA Source: SGD
    2. mitotic nuclear division Source: UniProtKB-KW
    3. nucleolus organization Source: SGD
    4. regulation of exit from mitosis Source: SGD

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31533-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nucleolar protein NET1
    Gene namesi
    Name:NET1
    Synonyms:CFI1, ESC5, SRM8
    Ordered Locus Names:YJL076W
    ORF Names:J1038
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome X

    Organism-specific databases

    CYGDiYJL076w.
    SGDiS000003612. NET1.

    Subcellular locationi

    Nucleusnucleolus 1 Publication

    GO - Cellular componenti

    1. nucleolus Source: SGD
    2. RENT complex Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11891189Nucleolar protein NET1PRO_0000096784Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei60 – 601Phosphoserine2 Publications
    Modified residuei166 – 1661Phosphoserine2 Publications
    Modified residuei231 – 2311Phosphoserine4 Publications
    Modified residuei252 – 2521Phosphoserine2 Publications
    Modified residuei437 – 4371Phosphoserine2 Publications
    Modified residuei439 – 4391Phosphoserine2 Publications
    Modified residuei447 – 4471Phosphoserine2 Publications
    Modified residuei452 – 4521Phosphoserine2 Publications
    Modified residuei497 – 4971Phosphoserine3 Publications
    Modified residuei676 – 6761Phosphothreonine2 Publications
    Modified residuei830 – 8301Phosphoserine2 Publications
    Modified residuei1042 – 10421Phosphothreonine3 Publications
    Modified residuei1056 – 10561Phosphoserine2 Publications
    Modified residuei1059 – 10591Phosphoserine2 Publications

    Post-translational modificationi

    Phosphorylated by CDC5.4 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP47035.
    PaxDbiP47035.
    PeptideAtlasiP47035.

    Expressioni

    Gene expression databases

    GenevestigatoriP47035.

    Interactioni

    Subunit structurei

    Component of the RENT complex which is composed of at least NET1, CDC14 and SIR2.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CDC14Q0068413EBI-25953,EBI-4192
    CDC8P005722EBI-25953,EBI-9957

    Protein-protein interaction databases

    BioGridi33680. 85 interactions.
    DIPiDIP-5153N.
    IntActiP47035. 95 interactions.
    MINTiMINT-500408.
    STRINGi4932.YJL076W.

    Structurei

    3D structure databases

    ProteinModelPortaliP47035.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi192 – 1998Poly-Thr
    Compositional biasi328 – 3358Poly-Ser
    Compositional biasi415 – 4184Poly-Ser
    Compositional biasi982 – 9887Poly-Ser
    Compositional biasi993 – 10008Poly-Ser
    Compositional biasi1171 – 11744Poly-Lys

    Sequence similaritiesi

    To yeast YKR010c.Curated

    Phylogenomic databases

    eggNOGiNOG12793.
    GeneTreeiENSGT00620000088873.
    KOiK06687.
    OMAiQSPPIRI.
    OrthoDBiEOG7DC2DV.

    Family and domain databases

    InterProiIPR018844. Dnt1-like_N.
    [Graphical view]
    PfamiPF10407. Cytokin_check_N. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P47035-1 [UniParc]FASTAAdd to Basket

    « Hide

    MYKLQVVLVP PSLQATMPIQ FGYGPTIAES SQLLPNRTNM AQSAGDASLQ     50
    YANLRSANVS FTPSYFNQSR FRKFLLFTKP TNTLLNLSDE IIDKCEKMYP 100
    SLQEDIEILS LQDNSGCDLD PDFLVKDVFN VNNIVRVILK NEIDLDDSAP 150
    VSLYKSVKRS KLNNGSPQSV QPQQQIPSSS GVLRIAKKRP PTGTTTTTTI 200
    RSATNGSMRV STPLARQIYP PPSSKIVSNN SDDEDEDIGE RSFLPPPTQP 250
    QSPPIRISSG IDAGKKIKSS IVEEDIVSRS ATVDPDKTKQ QRLLSGTPIM 300
    STMTPNRVTL TGQRVVSEHA HKNELVFSAS ASSSSFANGG TAAVTAQDIN 350
    RKPPVTTPRI TSGMLKIPEP RISEIEKELK EGPSSPASIL PAKAAKIPMK 400
    KPYLENGENY ESDDSSSSEN QETPETEPHS KASLQRSQSS IADNNGSPVK 450
    NSPLGDAMPH NVHLAELPKA SNTSITKSSN GESWGKQQEH QPPRKSSLET 500
    IVEKKSQAEP SGIVEPKRMT NFLDDNQVRE KEDTNDKLLE KEILPTIPHN 550
    DQPILASSDK SNGTLKSLAG KVSSNNNASK EDGTIINGTI EDDGNDNDEV 600
    DTTVRIVPQD SDSSSFPKSD LFKMIEGDDT DLPQWFKGKN SRTSGNSKNS 650
    KPYTTVLNKD IDNSKPDPRN ILPQRTPRSA AKRAAQLLAG AKKNEVPQKS 700
    TEDSSSAAST DDESESGIET DFSSDDDFKR KNMSVPNNGP KDISLHSLKG 750
    SVVPVKDSKI INKEVDEERN DKRDSQKKSA VSESSVTNSK ISEQMAKSFY 800
    PNSNKKQNEA TKVETKPATQ ASSFPVVGGS PSVATKGTTS FNEEGNRKNV 850
    KTKAKNESAQ IDRQQKETTS RVADLKSANI GGEDLNKKAE GSKEPEKASA 900
    NIQDANDKNN SKEKEDSKSK QVSQKKLKMT DHLKEGNVQL PKPSANDKLK 950
    DLKAKFTNSK TLVPPGIISN EKNNSSANDD DSSSSGSSTE DESSSSSSSS 1000
    DEETSTSRKA RRVVVNTPRE PVRSSSKIEA PSPSVNKKIN ATPDKIPVTQ 1050
    LMDMSSPPSV KSKTTSNPSS ILHDLPRKVR PSLSSLSDLV SRGIPDVKEK 1100
    TSKSNEKSQT KAPSSSDDES SSDSDSNSSS DSVSDSSSDS KSESDSDDSG 1150
    DSSDDGKSFI SAKSASAALG KKKKPSGGFA SLIKDFKKK 1189
    Length:1,189
    Mass (Da):128,531
    Last modified:February 1, 1996 - v1
    Checksum:i80EDF9A8065EB5A2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49351 Genomic DNA. Translation: CAA89367.1.
    BK006943 Genomic DNA. Translation: DAA08724.1.
    PIRiS56852.
    RefSeqiNP_012459.1. NM_001181509.1.

    Genome annotation databases

    EnsemblFungiiYJL076W; YJL076W; YJL076W.
    GeneIDi853369.
    KEGGisce:YJL076W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49351 Genomic DNA. Translation: CAA89367.1 .
    BK006943 Genomic DNA. Translation: DAA08724.1 .
    PIRi S56852.
    RefSeqi NP_012459.1. NM_001181509.1.

    3D structure databases

    ProteinModelPortali P47035.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33680. 85 interactions.
    DIPi DIP-5153N.
    IntActi P47035. 95 interactions.
    MINTi MINT-500408.
    STRINGi 4932.YJL076W.

    Proteomic databases

    MaxQBi P47035.
    PaxDbi P47035.
    PeptideAtlasi P47035.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YJL076W ; YJL076W ; YJL076W .
    GeneIDi 853369.
    KEGGi sce:YJL076W.

    Organism-specific databases

    CYGDi YJL076w.
    SGDi S000003612. NET1.

    Phylogenomic databases

    eggNOGi NOG12793.
    GeneTreei ENSGT00620000088873.
    KOi K06687.
    OMAi QSPPIRI.
    OrthoDBi EOG7DC2DV.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31533-MONOMER.

    Miscellaneous databases

    NextBioi 973807.

    Gene expression databases

    Genevestigatori P47035.

    Family and domain databases

    InterProi IPR018844. Dnt1-like_N.
    [Graphical view ]
    Pfami PF10407. Cytokin_check_N. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Budding yeast Cdc5 phosphorylates Net1 and assists Cdc14 release from the nucleolus."
      Yoshida S., Toh-e A.
      Biochem. Biophys. Res. Commun. 294:687-691(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, PHOSPHORYLATION.
    4. "Net1 stimulates RNA polymerase I transcription and regulates nucleolar structure independently of controlling mitotic exit."
      Shou W., Sakamoto K.M., Keener J., Morimoto K.W., Traverso E.E., Azzam R., Hoppe G.J., Feldman R.M.R., DeModena J., Moazed D., Charbonneau H., Nomura M., Deshaies R.J.
      Mol. Cell 8:45-55(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-231; SER-497 AND THR-676, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    7. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-231; SER-252; SER-830 AND THR-1042, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-60; SER-166; SER-231; SER-437; SER-439; SER-447; SER-452; SER-497; THR-1042; SER-1056 AND SER-1059, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiNET1_YEAST
    AccessioniPrimary (citable) accession number: P47035
    Secondary accession number(s): D6VWA8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1590 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    2. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3