Reviewed, UniProtKB/Swiss-Prot P47035 (NET1_YEAST)

Names and origin

Protein names

Recommended name:
    Nucleolar protein NET1

Gene names

Name:	NET1
Synonyms:	CFI1, ESC5, SRM8
Ordered Locus Names:	YJL076W
ORF Names:	J1038

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1189 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Has a role in chromosome maintenance and is involved in mitotic exit. Inhibits the action of CDC14 by sequestering it in the nucleolus. Also binds to RNA polymerase I and stimulates rRNA synthesis. Influences RDNA chromatin by tethering SIR2 to rDNA in the nucleolus. Ref.2 Ref.3
Subunit structure	Component of the RENT complex which is composed of at least NET1, CDC14 and SIR2. Ref.2 Ref.3
Subcellular location	Nucleus › nucleolus.
Post-translational modification	Phosphorylated by CDC5. Ref.2 Ref.5 Ref.6 Ref.7 Ref.8 Ref.9
Miscellaneous	Present with 1590 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	To yeast YKR010c.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell division Inferred from electronic annotation. Source: UniProtKB-KW chromatin silencing at rDNA Inferred from mutant phenotype. Source: SGD mitosis Inferred from electronic annotation. Source: UniProtKB-KW nucleolus organization Inferred from mutant phenotype. Source: SGD regulation of exit from mitosis Inferred from mutant phenotype. Source: SGD
Cellular component	RENT complex Inferred from direct assay. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct rDNA binding Inferred from direct assay. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CDC14	Q00684	8	EBI-25953,EBI-4192
CLB2	P24869	1	EBI-25953,EBI-4515
RPC40	P07703	1	EBI-25953,EBI-15831
RPO26	P20435	1	EBI-25953,EBI-15786
UTP7	P40055	1	EBI-25953,EBI-22597

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1189	1189	Nucleolar protein NET1		PRO_0000096784
Regions
	Compositional bias	192 – 199	8	Poly-Thr
	Compositional bias	328 – 335	8	Poly-Ser
	Compositional bias	415 – 418	4	Poly-Ser
	Compositional bias	982 – 988	7	Poly-Ser
	Compositional bias	993 – 1000	8	Poly-Ser
	Compositional bias	1171 – 1174	4	Poly-Lys
Amino acid modifications
	Modified residue	228	1	Phosphoserine Ref.9
	Modified residue	231	1	Phosphoserine Ref.6 Ref.9
	Modified residue	252	1	Phosphoserine Ref.5 Ref.9
	Modified residue	437	1	Phosphoserine Ref.9
	Modified residue	439	1	Phosphoserine Ref.9
	Modified residue	497	1	Phosphoserine Ref.5 Ref.6 Ref.7 Ref.8 Ref.9
	Modified residue	561	1	Phosphoserine Ref.8
	Modified residue	613	1	Phosphoserine Ref.9
	Modified residue	615	1	Phosphoserine Ref.9
	Modified residue	676	1	Phosphothreonine Ref.6 Ref.7
	Modified residue	747	1	Phosphoserine Ref.9
	Modified residue	823	1	Phosphoserine Ref.9
	Modified residue	830	1	Phosphoserine Ref.8 Ref.9
	Modified residue	840	1	Phosphoserine Ref.7 Ref.8 Ref.9
	Modified residue	1026	1	Phosphoserine Ref.9
	Modified residue	1032	1	Phosphoserine Ref.9
	Modified residue	1042	1	Phosphothreonine Ref.8 Ref.9
	Modified residue	1049	1	Phosphothreonine Ref.9
	Modified residue	1066	1	Phosphoserine Ref.9
	Modified residue	1082	1	Phosphoserine Ref.5 Ref.8 Ref.9
	Modified residue	1084	1	Phosphoserine Ref.8 Ref.9
	Modified residue	1085	1	Phosphoserine Ref.8 Ref.9

Sequences

	Sequence		Length	Mass (Da)
	P47035-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 80EDF9A8065EB5A2	FASTA	1,189	128,531
	10 20 30 40 50 60 MYKLQVVLVP PSLQATMPIQ FGYGPTIAES SQLLPNRTNM AQSAGDASLQ YANLRSANVS 70 80 90 100 110 120 FTPSYFNQSR FRKFLLFTKP TNTLLNLSDE IIDKCEKMYP SLQEDIEILS LQDNSGCDLD 130 140 150 160 170 180 PDFLVKDVFN VNNIVRVILK NEIDLDDSAP VSLYKSVKRS KLNNGSPQSV QPQQQIPSSS 190 200 210 220 230 240 GVLRIAKKRP PTGTTTTTTI RSATNGSMRV STPLARQIYP PPSSKIVSNN SDDEDEDIGE 250 260 270 280 290 300 RSFLPPPTQP QSPPIRISSG IDAGKKIKSS IVEEDIVSRS ATVDPDKTKQ QRLLSGTPIM 310 320 330 340 350 360 STMTPNRVTL TGQRVVSEHA HKNELVFSAS ASSSSFANGG TAAVTAQDIN RKPPVTTPRI 370 380 390 400 410 420 TSGMLKIPEP RISEIEKELK EGPSSPASIL PAKAAKIPMK KPYLENGENY ESDDSSSSEN 430 440 450 460 470 480 QETPETEPHS KASLQRSQSS IADNNGSPVK NSPLGDAMPH NVHLAELPKA SNTSITKSSN 490 500 510 520 530 540 GESWGKQQEH QPPRKSSLET IVEKKSQAEP SGIVEPKRMT NFLDDNQVRE KEDTNDKLLE 550 560 570 580 590 600 KEILPTIPHN DQPILASSDK SNGTLKSLAG KVSSNNNASK EDGTIINGTI EDDGNDNDEV 610 620 630 640 650 660 DTTVRIVPQD SDSSSFPKSD LFKMIEGDDT DLPQWFKGKN SRTSGNSKNS KPYTTVLNKD 670 680 690 700 710 720 IDNSKPDPRN ILPQRTPRSA AKRAAQLLAG AKKNEVPQKS TEDSSSAAST DDESESGIET 730 740 750 760 770 780 DFSSDDDFKR KNMSVPNNGP KDISLHSLKG SVVPVKDSKI INKEVDEERN DKRDSQKKSA 790 800 810 820 830 840 VSESSVTNSK ISEQMAKSFY PNSNKKQNEA TKVETKPATQ ASSFPVVGGS PSVATKGTTS 850 860 870 880 890 900 FNEEGNRKNV KTKAKNESAQ IDRQQKETTS RVADLKSANI GGEDLNKKAE GSKEPEKASA 910 920 930 940 950 960 NIQDANDKNN SKEKEDSKSK QVSQKKLKMT DHLKEGNVQL PKPSANDKLK DLKAKFTNSK 970 980 990 1000 1010 1020 TLVPPGIISN EKNNSSANDD DSSSSGSSTE DESSSSSSSS DEETSTSRKA RRVVVNTPRE 1030 1040 1050 1060 1070 1080 PVRSSSKIEA PSPSVNKKIN ATPDKIPVTQ LMDMSSPPSV KSKTTSNPSS ILHDLPRKVR 1090 1100 1110 1120 1130 1140 PSLSSLSDLV SRGIPDVKEK TSKSNEKSQT KAPSSSDDES SSDSDSNSSS DSVSDSSSDS 1150 1160 1170 1180 KSESDSDDSG DSSDDGKSFI SAKSASAALG KKKKPSGGFA SLIKDFKKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"Budding yeast Cdc5 phosphorylates Net1 and assists Cdc14 release from the nucleolus." Yoshida S., Toh-e A. Biochem. Biophys. Res. Commun. 294:687-691(2002) [PubMed: 12056824] [Abstract] Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, PHOSPHORYLATION.
[3]	"Net1 stimulates RNA polymerase I transcription and regulates nucleolar structure independently of controlling mitotic exit." Shou W., Sakamoto K.M., Keener J., Morimoto K.W., Traverso E.E., Azzam R., Hoppe G.J., Feldman R.M.R., DeModena J., Moazed D., Charbonneau H., Nomura M., Deshaies R.J. Mol. Cell 8:45-55(2001) [PubMed: 11511359] [Abstract] Cited for: FUNCTION, SUBUNIT.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-252; SER-497 AND SER-1082, MASS SPECTROMETRY.
[6]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-231; SER-497 AND THR-676, MASS SPECTROMETRY.
[7]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-497; THR-676 AND SER-840, MASS SPECTROMETRY.
[8]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-497; SER-561; SER-830; SER-840; THR-1042; SER-1082; SER-1084 AND SER-1085, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-228; SER-231; SER-252; SER-437; SER-439; SER-497; SER-613; SER-615; SER-747; SER-823; SER-830; SER-840; SER-1026; SER-1032; THR-1042; THR-1049; SER-1066; SER-1082; SER-1084 AND SER-1085, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z49351 Genomic DNA. Translation: CAA89367.1.
PIR	S56852.
RefSeq	NP_012459.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:5153N.
IntAct	P47035. 18 interactions.
STRING	P47035.
Proteomic databases
PeptideAtlas	P47035.
Genome annotation databases
Ensembl	YJL076W; YJL076W; YJL076W; Saccharomyces cerevisiae. [Genome view]
GeneID	853369.
KEGG	sce:YJL076W.
NMPDR	fig|4932.3.peg.3430.
Organism-specific databases
CYGD	YJL076w.
SGD	S000003612. NET1.
Phylogenomic databases
HOGENOM	P47035.
OMA	STEPKST
OrthoDB	EOG9PCC8Q
Gene expression databases
ArrayExpress	P47035.
Genevestigator	P47035.
GermOnline	YJL076W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR018844. SIN_reg_Dnt1_N. [Graphical view]
Pfam	PF10407. Cytokin_check_N. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	973807.

Entry information

Entry name

NET1_YEAST

Accession

Primary (citable) accession number: P47035

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	November 24, 2009
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)