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P47035 (NET1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nucleolar protein NET1
Gene names
Name:NET1
Synonyms:CFI1, ESC5, SRM8
Ordered Locus Names:YJL076W
ORF Names:J1038
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1189 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Has a role in chromosome maintenance and is involved in mitotic exit. Inhibits the action of CDC14 by sequestering it in the nucleolus. Also binds to RNA polymerase I and stimulates rRNA synthesis. Influences RDNA chromatin by tethering SIR2 to rDNA in the nucleolus. Ref.3 Ref.4

Subunit structure

Component of the RENT complex which is composed of at least NET1, CDC14 and SIR2. Ref.3 Ref.4

Subcellular location

Nucleusnucleolus Ref.3.

Post-translational modification

Phosphorylated by CDC5. Ref.3

Miscellaneous

Present with 1590 molecules/cell in log phase SD medium.

Sequence similarities

To yeast YKR010c.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CDC14Q0068413EBI-25953,EBI-4192
CDC8P005722EBI-25953,EBI-9957

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11891189Nucleolar protein NET1
PRO_0000096784

Regions

Compositional bias192 – 1998Poly-Thr
Compositional bias328 – 3358Poly-Ser
Compositional bias415 – 4184Poly-Ser
Compositional bias982 – 9887Poly-Ser
Compositional bias993 – 10008Poly-Ser
Compositional bias1171 – 11744Poly-Lys

Amino acid modifications

Modified residue601Phosphoserine Ref.9
Modified residue1661Phosphoserine Ref.9
Modified residue2311Phosphoserine Ref.6 Ref.8 Ref.9
Modified residue2521Phosphoserine Ref.8
Modified residue4371Phosphoserine Ref.9
Modified residue4391Phosphoserine Ref.9
Modified residue4471Phosphoserine Ref.9
Modified residue4521Phosphoserine Ref.9
Modified residue4971Phosphoserine Ref.6 Ref.9
Modified residue6761Phosphothreonine Ref.6
Modified residue8301Phosphoserine Ref.8
Modified residue10421Phosphothreonine Ref.8 Ref.9
Modified residue10561Phosphoserine Ref.9
Modified residue10591Phosphoserine Ref.9

Sequences

Sequence LengthMass (Da)Tools
P47035 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: 80EDF9A8065EB5A2

FASTA1,189128,531
        10         20         30         40         50         60 
MYKLQVVLVP PSLQATMPIQ FGYGPTIAES SQLLPNRTNM AQSAGDASLQ YANLRSANVS 

        70         80         90        100        110        120 
FTPSYFNQSR FRKFLLFTKP TNTLLNLSDE IIDKCEKMYP SLQEDIEILS LQDNSGCDLD 

       130        140        150        160        170        180 
PDFLVKDVFN VNNIVRVILK NEIDLDDSAP VSLYKSVKRS KLNNGSPQSV QPQQQIPSSS 

       190        200        210        220        230        240 
GVLRIAKKRP PTGTTTTTTI RSATNGSMRV STPLARQIYP PPSSKIVSNN SDDEDEDIGE 

       250        260        270        280        290        300 
RSFLPPPTQP QSPPIRISSG IDAGKKIKSS IVEEDIVSRS ATVDPDKTKQ QRLLSGTPIM 

       310        320        330        340        350        360 
STMTPNRVTL TGQRVVSEHA HKNELVFSAS ASSSSFANGG TAAVTAQDIN RKPPVTTPRI 

       370        380        390        400        410        420 
TSGMLKIPEP RISEIEKELK EGPSSPASIL PAKAAKIPMK KPYLENGENY ESDDSSSSEN 

       430        440        450        460        470        480 
QETPETEPHS KASLQRSQSS IADNNGSPVK NSPLGDAMPH NVHLAELPKA SNTSITKSSN 

       490        500        510        520        530        540 
GESWGKQQEH QPPRKSSLET IVEKKSQAEP SGIVEPKRMT NFLDDNQVRE KEDTNDKLLE 

       550        560        570        580        590        600 
KEILPTIPHN DQPILASSDK SNGTLKSLAG KVSSNNNASK EDGTIINGTI EDDGNDNDEV 

       610        620        630        640        650        660 
DTTVRIVPQD SDSSSFPKSD LFKMIEGDDT DLPQWFKGKN SRTSGNSKNS KPYTTVLNKD 

       670        680        690        700        710        720 
IDNSKPDPRN ILPQRTPRSA AKRAAQLLAG AKKNEVPQKS TEDSSSAAST DDESESGIET 

       730        740        750        760        770        780 
DFSSDDDFKR KNMSVPNNGP KDISLHSLKG SVVPVKDSKI INKEVDEERN DKRDSQKKSA 

       790        800        810        820        830        840 
VSESSVTNSK ISEQMAKSFY PNSNKKQNEA TKVETKPATQ ASSFPVVGGS PSVATKGTTS 

       850        860        870        880        890        900 
FNEEGNRKNV KTKAKNESAQ IDRQQKETTS RVADLKSANI GGEDLNKKAE GSKEPEKASA 

       910        920        930        940        950        960 
NIQDANDKNN SKEKEDSKSK QVSQKKLKMT DHLKEGNVQL PKPSANDKLK DLKAKFTNSK 

       970        980        990       1000       1010       1020 
TLVPPGIISN EKNNSSANDD DSSSSGSSTE DESSSSSSSS DEETSTSRKA RRVVVNTPRE 

      1030       1040       1050       1060       1070       1080 
PVRSSSKIEA PSPSVNKKIN ATPDKIPVTQ LMDMSSPPSV KSKTTSNPSS ILHDLPRKVR 

      1090       1100       1110       1120       1130       1140 
PSLSSLSDLV SRGIPDVKEK TSKSNEKSQT KAPSSSDDES SSDSDSNSSS DSVSDSSSDS 

      1150       1160       1170       1180 
KSESDSDDSG DSSDDGKSFI SAKSASAALG KKKKPSGGFA SLIKDFKKK 

« Hide

References

« Hide 'large scale' references
[1]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Budding yeast Cdc5 phosphorylates Net1 and assists Cdc14 release from the nucleolus."
Yoshida S., Toh-e A.
Biochem. Biophys. Res. Commun. 294:687-691(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, PHOSPHORYLATION.
[4]"Net1 stimulates RNA polymerase I transcription and regulates nucleolar structure independently of controlling mitotic exit."
Shou W., Sakamoto K.M., Keener J., Morimoto K.W., Traverso E.E., Azzam R., Hoppe G.J., Feldman R.M.R., DeModena J., Moazed D., Charbonneau H., Nomura M., Deshaies R.J.
Mol. Cell 8:45-55(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-231; SER-497 AND THR-676, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[7]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-231; SER-252; SER-830 AND THR-1042, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-60; SER-166; SER-231; SER-437; SER-439; SER-447; SER-452; SER-497; THR-1042; SER-1056 AND SER-1059, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z49351 Genomic DNA. Translation: CAA89367.1.
BK006943 Genomic DNA. Translation: DAA08724.1.
PIRS56852.
RefSeqNP_012459.1. NM_001181509.1.

3D structure databases

ProteinModelPortalP47035.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33680. 85 interactions.
DIPDIP-5153N.
IntActP47035. 95 interactions.
MINTMINT-500408.
STRING4932.YJL076W.

Proteomic databases

MaxQBP47035.
PaxDbP47035.
PeptideAtlasP47035.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJL076W; YJL076W; YJL076W.
GeneID853369.
KEGGsce:YJL076W.

Organism-specific databases

CYGDYJL076w.
SGDS000003612. NET1.

Phylogenomic databases

eggNOGNOG12793.
GeneTreeENSGT00620000088873.
KOK06687.
OMAQSPPIRI.
OrthoDBEOG7DC2DV.

Enzyme and pathway databases

BioCycYEAST:G3O-31533-MONOMER.

Gene expression databases

GenevestigatorP47035.

Family and domain databases

InterProIPR018844. Dnt1-like_N.
[Graphical view]
PfamPF10407. Cytokin_check_N. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio973807.

Entry information

Entry nameNET1_YEAST
AccessionPrimary (citable) accession number: P47035
Secondary accession number(s): D6VWA8
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: June 11, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD