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Protein

Nucleolar protein NET1

Gene

NET1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has a role in chromosome maintenance and is involved in mitotic exit. Inhibits the action of CDC14 by sequestering it in the nucleolus. Also binds to RNA polymerase I and stimulates rRNA synthesis. Influences RDNA chromatin by tethering SIR2 to rDNA in the nucleolus.2 Publications

GO - Molecular functioni

  1. rDNA binding Source: SGD

GO - Biological processi

  1. cell division Source: UniProtKB-KW
  2. chromatin silencing at rDNA Source: SGD
  3. mitotic nuclear division Source: UniProtKB-KW
  4. nucleolus organization Source: SGD
  5. regulation of exit from mitosis Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-31533-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleolar protein NET1
Gene namesi
Name:NET1
Synonyms:CFI1, ESC5, SRM8
Ordered Locus Names:YJL076W
ORF Names:J1038
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome X

Organism-specific databases

CYGDiYJL076w.
SGDiS000003612. NET1.

Subcellular locationi

Nucleusnucleolus 1 Publication

GO - Cellular componenti

  1. nucleolus Source: SGD
  2. RENT complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11891189Nucleolar protein NET1PRO_0000096784Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei60 – 601Phosphoserine1 Publication
Modified residuei166 – 1661Phosphoserine1 Publication
Modified residuei231 – 2311Phosphoserine3 Publications
Modified residuei252 – 2521Phosphoserine1 Publication
Modified residuei437 – 4371Phosphoserine1 Publication
Modified residuei439 – 4391Phosphoserine1 Publication
Modified residuei447 – 4471Phosphoserine1 Publication
Modified residuei452 – 4521Phosphoserine1 Publication
Modified residuei497 – 4971Phosphoserine2 Publications
Modified residuei676 – 6761Phosphothreonine1 Publication
Modified residuei830 – 8301Phosphoserine1 Publication
Modified residuei1042 – 10421Phosphothreonine2 Publications
Modified residuei1056 – 10561Phosphoserine1 Publication
Modified residuei1059 – 10591Phosphoserine1 Publication

Post-translational modificationi

Phosphorylated by CDC5.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP47035.
PaxDbiP47035.
PeptideAtlasiP47035.

Expressioni

Gene expression databases

GenevestigatoriP47035.

Interactioni

Subunit structurei

Component of the RENT complex which is composed of at least NET1, CDC14 and SIR2 (PubMed:12056824, PubMed:11511359). Interacts with NSI1 (PubMed:22362748).3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CDC14Q0068413EBI-25953,EBI-4192
CDC8P005722EBI-25953,EBI-9957

Protein-protein interaction databases

BioGridi33680. 87 interactions.
DIPiDIP-5153N.
IntActiP47035. 95 interactions.
MINTiMINT-500408.
STRINGi4932.YJL076W.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi192 – 1998Poly-Thr
Compositional biasi328 – 3358Poly-Ser
Compositional biasi415 – 4184Poly-Ser
Compositional biasi982 – 9887Poly-Ser
Compositional biasi993 – 10008Poly-Ser
Compositional biasi1171 – 11744Poly-Lys

Sequence similaritiesi

To yeast YKR010c.Curated

Phylogenomic databases

eggNOGiNOG12793.
GeneTreeiENSGT00620000088873.
InParanoidiP47035.
KOiK06687.
OMAiFQKSELL.
OrthoDBiEOG7DC2DV.

Family and domain databases

InterProiIPR018844. Dnt1-like_N.
[Graphical view]
PfamiPF10407. Cytokin_check_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47035-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYKLQVVLVP PSLQATMPIQ FGYGPTIAES SQLLPNRTNM AQSAGDASLQ
60 70 80 90 100
YANLRSANVS FTPSYFNQSR FRKFLLFTKP TNTLLNLSDE IIDKCEKMYP
110 120 130 140 150
SLQEDIEILS LQDNSGCDLD PDFLVKDVFN VNNIVRVILK NEIDLDDSAP
160 170 180 190 200
VSLYKSVKRS KLNNGSPQSV QPQQQIPSSS GVLRIAKKRP PTGTTTTTTI
210 220 230 240 250
RSATNGSMRV STPLARQIYP PPSSKIVSNN SDDEDEDIGE RSFLPPPTQP
260 270 280 290 300
QSPPIRISSG IDAGKKIKSS IVEEDIVSRS ATVDPDKTKQ QRLLSGTPIM
310 320 330 340 350
STMTPNRVTL TGQRVVSEHA HKNELVFSAS ASSSSFANGG TAAVTAQDIN
360 370 380 390 400
RKPPVTTPRI TSGMLKIPEP RISEIEKELK EGPSSPASIL PAKAAKIPMK
410 420 430 440 450
KPYLENGENY ESDDSSSSEN QETPETEPHS KASLQRSQSS IADNNGSPVK
460 470 480 490 500
NSPLGDAMPH NVHLAELPKA SNTSITKSSN GESWGKQQEH QPPRKSSLET
510 520 530 540 550
IVEKKSQAEP SGIVEPKRMT NFLDDNQVRE KEDTNDKLLE KEILPTIPHN
560 570 580 590 600
DQPILASSDK SNGTLKSLAG KVSSNNNASK EDGTIINGTI EDDGNDNDEV
610 620 630 640 650
DTTVRIVPQD SDSSSFPKSD LFKMIEGDDT DLPQWFKGKN SRTSGNSKNS
660 670 680 690 700
KPYTTVLNKD IDNSKPDPRN ILPQRTPRSA AKRAAQLLAG AKKNEVPQKS
710 720 730 740 750
TEDSSSAAST DDESESGIET DFSSDDDFKR KNMSVPNNGP KDISLHSLKG
760 770 780 790 800
SVVPVKDSKI INKEVDEERN DKRDSQKKSA VSESSVTNSK ISEQMAKSFY
810 820 830 840 850
PNSNKKQNEA TKVETKPATQ ASSFPVVGGS PSVATKGTTS FNEEGNRKNV
860 870 880 890 900
KTKAKNESAQ IDRQQKETTS RVADLKSANI GGEDLNKKAE GSKEPEKASA
910 920 930 940 950
NIQDANDKNN SKEKEDSKSK QVSQKKLKMT DHLKEGNVQL PKPSANDKLK
960 970 980 990 1000
DLKAKFTNSK TLVPPGIISN EKNNSSANDD DSSSSGSSTE DESSSSSSSS
1010 1020 1030 1040 1050
DEETSTSRKA RRVVVNTPRE PVRSSSKIEA PSPSVNKKIN ATPDKIPVTQ
1060 1070 1080 1090 1100
LMDMSSPPSV KSKTTSNPSS ILHDLPRKVR PSLSSLSDLV SRGIPDVKEK
1110 1120 1130 1140 1150
TSKSNEKSQT KAPSSSDDES SSDSDSNSSS DSVSDSSSDS KSESDSDDSG
1160 1170 1180
DSSDDGKSFI SAKSASAALG KKKKPSGGFA SLIKDFKKK
Length:1,189
Mass (Da):128,531
Last modified:January 31, 1996 - v1
Checksum:i80EDF9A8065EB5A2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49351 Genomic DNA. Translation: CAA89367.1.
BK006943 Genomic DNA. Translation: DAA08724.1.
PIRiS56852.
RefSeqiNP_012459.1. NM_001181509.1.

Genome annotation databases

EnsemblFungiiYJL076W; YJL076W; YJL076W.
GeneIDi853369.
KEGGisce:YJL076W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49351 Genomic DNA. Translation: CAA89367.1.
BK006943 Genomic DNA. Translation: DAA08724.1.
PIRiS56852.
RefSeqiNP_012459.1. NM_001181509.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33680. 87 interactions.
DIPiDIP-5153N.
IntActiP47035. 95 interactions.
MINTiMINT-500408.
STRINGi4932.YJL076W.

Proteomic databases

MaxQBiP47035.
PaxDbiP47035.
PeptideAtlasiP47035.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJL076W; YJL076W; YJL076W.
GeneIDi853369.
KEGGisce:YJL076W.

Organism-specific databases

CYGDiYJL076w.
SGDiS000003612. NET1.

Phylogenomic databases

eggNOGiNOG12793.
GeneTreeiENSGT00620000088873.
InParanoidiP47035.
KOiK06687.
OMAiFQKSELL.
OrthoDBiEOG7DC2DV.

Enzyme and pathway databases

BioCyciYEAST:G3O-31533-MONOMER.

Miscellaneous databases

NextBioi973807.

Gene expression databases

GenevestigatoriP47035.

Family and domain databases

InterProiIPR018844. Dnt1-like_N.
[Graphical view]
PfamiPF10407. Cytokin_check_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Budding yeast Cdc5 phosphorylates Net1 and assists Cdc14 release from the nucleolus."
    Yoshida S., Toh-e A.
    Biochem. Biophys. Res. Commun. 294:687-691(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, PHOSPHORYLATION.
  4. "Net1 stimulates RNA polymerase I transcription and regulates nucleolar structure independently of controlling mitotic exit."
    Shou W., Sakamoto K.M., Keener J., Morimoto K.W., Traverso E.E., Azzam R., Hoppe G.J., Feldman R.M.R., DeModena J., Moazed D., Charbonneau H., Nomura M., Deshaies R.J.
    Mol. Cell 8:45-55(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-231; SER-497 AND THR-676, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  7. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-231; SER-252; SER-830 AND THR-1042, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-60; SER-166; SER-231; SER-437; SER-439; SER-447; SER-452; SER-497; THR-1042; SER-1056 AND SER-1059, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Nsi1 plays a significant role in the silencing of ribosomal DNA in Saccharomyces cerevisiae."
    Ha C.W., Sung M.K., Huh W.K.
    Nucleic Acids Res. 40:4892-4903(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NSI1.

Entry informationi

Entry nameiNET1_YEAST
AccessioniPrimary (citable) accession number: P47035
Secondary accession number(s): D6VWA8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 31, 1996
Last sequence update: January 31, 1996
Last modified: March 31, 2015
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1590 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.