Nucleolar protein NET1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P47035 (NET1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 106. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Nucleolar protein NET1

Gene names

Name:	NET1
Synonyms:	CFI1, ESC5, SRM8
Ordered Locus Names:	YJL076W
ORF Names:	J1038

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	1189 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has a role in chromosome maintenance and is involved in mitotic exit. Inhibits the action of CDC14 by sequestering it in the nucleolus. Also binds to RNA polymerase I and stimulates rRNA synthesis. Influences RDNA chromatin by tethering SIR2 to rDNA in the nucleolus. Ref.3 Ref.4
Subunit structure	Component of the RENT complex which is composed of at least NET1, CDC14 and SIR2. Ref.3 Ref.4
Subcellular location	Nucleus › nucleolus Ref.3.
Post-translational modification	Phosphorylated by CDC5. Ref.3
Miscellaneous	Present with 1590 molecules/cell in log phase SD medium.
Sequence similarities	To yeast YKR010c.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell division Inferred from electronic annotation. Source: UniProtKB-KW chromatin silencing at rDNA Inferred from physical interaction PubMed 10219245. Source: SGD mitosis Inferred from electronic annotation. Source: UniProtKB-KW nucleolus organization Inferred from mutant phenotype PubMed 10219245. Source: SGD regulation of exit from mitosis Inferred from mutant phenotype PubMed 10219244. Source: SGD
Cellular_component	RENT complex Inferred from direct assay PubMed 10219244. Source: SGD
Molecular_function	rDNA binding Inferred from direct assay PubMed 10219245. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CDC14	Q00684	13	EBI-25953,EBI-4192
CDC8	P00572	2	EBI-25953,EBI-9957

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1189

1189

Nucleolar protein NET1

PRO_0000096784

Regions

Compositional bias

192 – 199

Poly-Thr

Compositional bias

328 – 335

Poly-Ser

Compositional bias

415 – 418

Poly-Ser

Compositional bias

982 – 988

Poly-Ser

Compositional bias

993 – 1000

Poly-Ser

Compositional bias

1171 – 1174

Poly-Lys

Amino acid modifications

Modified residue

228

Phosphoserine Ref.10

Modified residue

231

Phosphoserine Ref.7 Ref.10

Modified residue

252

Phosphoserine Ref.6 Ref.10

Modified residue

437

Phosphoserine Ref.10

Modified residue

439

Phosphoserine Ref.10

Modified residue

497

Phosphoserine Ref.6 Ref.7 Ref.8 Ref.9 Ref.10

Modified residue

561

Phosphoserine Ref.9

Modified residue

613

Phosphoserine Ref.10

Modified residue

615

Phosphoserine Ref.10

Modified residue

676

Phosphothreonine Ref.7 Ref.8

Modified residue

747

Phosphoserine Ref.10

Modified residue

823

Phosphoserine Ref.10

Modified residue

830

Phosphoserine Ref.9 Ref.10

Modified residue

840

Phosphoserine Ref.8 Ref.9 Ref.10

Modified residue

1026

Phosphoserine Ref.10

Modified residue

1032

Phosphoserine Ref.10

Modified residue

1042

Phosphothreonine Ref.9 Ref.10

Modified residue

1049

Phosphothreonine Ref.10

Modified residue

1066

Phosphoserine Ref.10

Modified residue

1082

Phosphoserine Ref.6 Ref.9 Ref.10

Modified residue

1084

Phosphoserine Ref.9 Ref.10

Modified residue

1085

Phosphoserine Ref.9 Ref.10

Sequences

Sequence

Length

Mass (Da)

Tools

P47035 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: 80EDF9A8065EB5A2
Show »

FASTA

1,189

128,531

        10         20         30         40         50         60 
MYKLQVVLVP PSLQATMPIQ FGYGPTIAES SQLLPNRTNM AQSAGDASLQ YANLRSANVS 

        70         80         90        100        110        120 
FTPSYFNQSR FRKFLLFTKP TNTLLNLSDE IIDKCEKMYP SLQEDIEILS LQDNSGCDLD 

       130        140        150        160        170        180 
PDFLVKDVFN VNNIVRVILK NEIDLDDSAP VSLYKSVKRS KLNNGSPQSV QPQQQIPSSS 

       190        200        210        220        230        240 
GVLRIAKKRP PTGTTTTTTI RSATNGSMRV STPLARQIYP PPSSKIVSNN SDDEDEDIGE 

       250        260        270        280        290        300 
RSFLPPPTQP QSPPIRISSG IDAGKKIKSS IVEEDIVSRS ATVDPDKTKQ QRLLSGTPIM 

       310        320        330        340        350        360 
STMTPNRVTL TGQRVVSEHA HKNELVFSAS ASSSSFANGG TAAVTAQDIN RKPPVTTPRI 

       370        380        390        400        410        420 
TSGMLKIPEP RISEIEKELK EGPSSPASIL PAKAAKIPMK KPYLENGENY ESDDSSSSEN 

       430        440        450        460        470        480 
QETPETEPHS KASLQRSQSS IADNNGSPVK NSPLGDAMPH NVHLAELPKA SNTSITKSSN 

       490        500        510        520        530        540 
GESWGKQQEH QPPRKSSLET IVEKKSQAEP SGIVEPKRMT NFLDDNQVRE KEDTNDKLLE 

       550        560        570        580        590        600 
KEILPTIPHN DQPILASSDK SNGTLKSLAG KVSSNNNASK EDGTIINGTI EDDGNDNDEV 

       610        620        630        640        650        660 
DTTVRIVPQD SDSSSFPKSD LFKMIEGDDT DLPQWFKGKN SRTSGNSKNS KPYTTVLNKD 

       670        680        690        700        710        720 
IDNSKPDPRN ILPQRTPRSA AKRAAQLLAG AKKNEVPQKS TEDSSSAAST DDESESGIET 

       730        740        750        760        770        780 
DFSSDDDFKR KNMSVPNNGP KDISLHSLKG SVVPVKDSKI INKEVDEERN DKRDSQKKSA 

       790        800        810        820        830        840 
VSESSVTNSK ISEQMAKSFY PNSNKKQNEA TKVETKPATQ ASSFPVVGGS PSVATKGTTS 

       850        860        870        880        890        900 
FNEEGNRKNV KTKAKNESAQ IDRQQKETTS RVADLKSANI GGEDLNKKAE GSKEPEKASA 

       910        920        930        940        950        960 
NIQDANDKNN SKEKEDSKSK QVSQKKLKMT DHLKEGNVQL PKPSANDKLK DLKAKFTNSK 

       970        980        990       1000       1010       1020 
TLVPPGIISN EKNNSSANDD DSSSSGSSTE DESSSSSSSS DEETSTSRKA RRVVVNTPRE 

      1030       1040       1050       1060       1070       1080 
PVRSSSKIEA PSPSVNKKIN ATPDKIPVTQ LMDMSSPPSV KSKTTSNPSS ILHDLPRKVR 

      1090       1100       1110       1120       1130       1140 
PSLSSLSDLV SRGIPDVKEK TSKSNEKSQT KAPSSSDDES SSDSDSNSSS DSVSDSSSDS 

      1150       1160       1170       1180 
KSESDSDDSG DSSDDGKSFI SAKSASAALG KKKKPSGGFA SLIKDFKKK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Budding yeast Cdc5 phosphorylates Net1 and assists Cdc14 release from the nucleolus." Yoshida S., Toh-e A. Biochem. Biophys. Res. Commun. 294:687-691(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, PHOSPHORYLATION.
[4]	"Net1 stimulates RNA polymerase I transcription and regulates nucleolar structure independently of controlling mitotic exit." Shou W., Sakamoto K.M., Keener J., Morimoto K.W., Traverso E.E., Azzam R., Hoppe G.J., Feldman R.M.R., DeModena J., Moazed D., Charbonneau H., Nomura M., Deshaies R.J. Mol. Cell 8:45-55(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBUNIT.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-252; SER-497 AND SER-1082, MASS SPECTROMETRY. Strain: YAL6B.
[7]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-231; SER-497 AND THR-676, MASS SPECTROMETRY. Strain: ADR376.
[8]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-497; THR-676 AND SER-840, MASS SPECTROMETRY.
[9]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-497; SER-561; SER-830; SER-840; THR-1042; SER-1082; SER-1084 AND SER-1085, MASS SPECTROMETRY.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-228; SER-231; SER-252; SER-437; SER-439; SER-497; SER-613; SER-615; SER-747; SER-823; SER-830; SER-840; SER-1026; SER-1032; THR-1042; THR-1049; SER-1066; SER-1082; SER-1084 AND SER-1085, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z49351 Genomic DNA. Translation: CAA89367.1. BK006943 Genomic DNA. Translation: DAA08724.1.
PIR	S56852.
RefSeq	NP_012459.1. NM_001181509.1.
3D structure databases
ProteinModelPortal	P47035.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5153N.
IntAct	P47035. 96 interactions.
MINT	MINT-500408.
STRING	4932.YJL076W.
Proteomic databases
PaxDb	P47035.
PeptideAtlas	P47035.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJL076W; YJL076W; YJL076W.
GeneID	853369.
KEGG	sce:YJL076W.
Organism-specific databases
CYGD	YJL076w.
SGD	S000003612. NET1.
Phylogenomic databases
eggNOG	NOG12793.
GeneTree	ENSGT00620000088873.
KO	K06687.
OMA	SEPKNSE.
OrthoDB	EOG4G7G7P.
Enzyme and pathway databases
BioCyc	YEAST:G3O-31533-MONOMER.
Gene expression databases
Genevestigator	P47035.
GermOnline	YJL076W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR018844. Dnt1-like_N. [Graphical view]
Pfam	PF10407. Cytokin_check_N. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	973807.

Entry information

Entry name

NET1_YEAST

Accession

Primary (citable) accession number: P47035
Secondary accession number(s): D6VWA8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	May 29, 2013
This is version 106 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents