Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P47032 (PRY1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein PRY1
Alternative name(s):
Pathogenesis-related protein 1
Gene names
Name:PRY1
Ordered Locus Names:YJL079C
ORF Names:J1022
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length299 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Secreted protein required for efficient export of lipids such as acetylated sterols. Acts in detoxification of hydrophobic compounds. Ref.7

Subcellular location

Secreted Ref.5 Ref.7.

Domain

The SCP domain is necessary and sufficient for lipid export and sterol-binding (Ref.7).

Post-translational modification

O-glycosylated. Ref.7

Miscellaneous

Present with 556 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the CRISP family.

Contains 1 SCP domain.

Ontologies

Keywords
   Biological processLipid transport
Transport
   Cellular componentSecreted
   DomainSignal
   LigandLipid-binding
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processsterol transport

Inferred from mutant phenotype Ref.7. Source: SGD

   Cellular_componentextracellular region

Inferred from direct assay Ref.5. Source: SGD

   Molecular_functionsterol binding

Inferred from direct assay Ref.7. Source: SGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Chain20 – 299280Protein PRY1
PRO_0000006317

Regions

Domain167 – 281115SCP
Compositional bias102 – 16564Ala/Ser/Thr-rich

Sequences

Sequence LengthMass (Da)Tools
P47032 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: A316769CA87C5679

FASTA29930,634
        10         20         30         40         50         60 
MKLSKLSILT SALATSALAA PAVVTVTEHA HEAAVVTVQG VVYVENGQTR TTYETLAPAS 

        70         80         90        100        110        120 
TATPTSTATA LVAPPVAPSS ASSNSDVVLS ALKNLASVWG KTTDSTTTLT SSESTSQSLA 

       130        140        150        160        170        180 
QATTTSTPAA ASTTSTPAAT TTTSQAAATS SASSSDSDLS DFASSVLAEH NKKRALHKDT 

       190        200        210        220        230        240 
PALSWSDTLA SYAQDYADNY DCSGTLTHSG GPYGENLALG YDGPAAVDAW YNEISNYDFS 

       250        260        270        280        290 
NPGFSSNTGH FTQVVWKSTT QVGCGIKTCG GAWGDYVICS YDPAGNYEGE YADNVEPLA 

« Hide

References

« Hide 'large scale' references
[1]"Sequence analysis of a 33.1 kb fragment from the left arm of Saccharomyces cerevisiae chromosome X, including putative proteins with leucine zippers, a fungal Zn(II)2-Cys6 binuclear cluster domain and a putative alpha 2-SCB-alpha 2 binding site."
Miosga T., Schaaff-Gerstenschlaeger I., Chalwatzis N., Baur A., Boles E., Fournier C., Schmitt S., Velten C., Wilhelm N., Zimmermann F.K.
Yeast 11:681-689(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Sequence-based approach for identification of cell wall proteins in Saccharomyces cerevisiae."
Terashima H., Fukuchi S., Nakai K., Arisawa M., Hamada K., Yabuki N., Kitada K.
Curr. Genet. 40:311-316(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Pathogen-Related Yeast (PRY) proteins and members of the CAP superfamily are secreted sterol-binding proteins."
Choudhary V., Schneiter R.
Proc. Natl. Acad. Sci. U.S.A. 109:16882-16887(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, STEROL-BINDING, SUBCELLULAR LOCATION, GLYCOSYLATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z49354 Genomic DNA. Translation: CAA89372.1.
X83502 Genomic DNA. Translation: CAA58491.1.
X88851 Genomic DNA. Translation: CAA61315.1.
AY558306 Genomic DNA. Translation: AAS56632.1.
BK006943 Genomic DNA. Translation: DAA08720.1.
PIRS56031.
RefSeqNP_012456.1. NM_001181512.1.

3D structure databases

ProteinModelPortalP47032.
SMRP47032. Positions 162-297.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33677. 24 interactions.
DIPDIP-3857N.
IntActP47032. 1 interaction.
MINTMINT-557532.
STRING4932.YJL079C.

Proteomic databases

MaxQBP47032.
PaxDbP47032.
PeptideAtlasP47032.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJL079C; YJL079C; YJL079C.
GeneID853366.
KEGGsce:YJL079C.

Organism-specific databases

CYGDYJL079c.
SGDS000003615. PRY1.

Phylogenomic databases

eggNOGCOG2340.
GeneTreeENSGT00390000020276.
OMAYNHNIHR.
OrthoDBEOG7HHX5C.

Enzyme and pathway databases

BioCycYEAST:G3O-31536-MONOMER.

Gene expression databases

GenevestigatorP47032.

Family and domain databases

Gene3D3.40.33.10. 1 hit.
InterProIPR001283. Allrgn_V5/Tpx1.
IPR018244. Allrgn_V5/Tpx1_CS.
IPR014044. CAP_domain.
[Graphical view]
PANTHERPTHR10334. PTHR10334. 1 hit.
PfamPF00188. CAP. 1 hit.
[Graphical view]
PRINTSPR00837. V5TPXLIKE.
SMARTSM00198. SCP. 1 hit.
[Graphical view]
SUPFAMSSF55797. SSF55797. 1 hit.
PROSITEPS01009. CRISP_1. 1 hit.
PS01010. CRISP_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio973798.
PMAP-CutDBP47032.

Entry information

Entry namePRY1_YEAST
AccessionPrimary (citable) accession number: P47032
Secondary accession number(s): D6VWA4
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: May 14, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families