P47030 (TAX4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein TAX4

Gene names

Name:	TAX4
Ordered Locus Names:	YJL083W
ORF Names:	J1002

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	604 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	With IRS4, acts as a positive regulator of INP51 activity and phosphatidylinositol 4,5-bisphosphate turnover. Negatively regulates signaling through the cell integrity pathway, including the MAP kinase SLT2. Ref.6
Subunit structure	Interacts with INP51. Ref.6
Miscellaneous	Present with 396 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the IRS4 family. Contains 1 EH domain.

Ontologies

Keywords
Biological process	Lipid metabolism
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	autophagy Inferred from genetic interaction. Source: SGD cellular response to starvation Inferred from genetic interaction. Source: SGD fungal-type cell wall organization Inferred from genetic interaction Ref.6. Source: SGD inositol lipid-mediated signaling Inferred from genetic interaction Ref.6. Source: SGD lipid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	pre-autophagosomal structure Inferred from direct assay. Source: SGD
Molecular function	calcium ion binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction Ref.6. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
INP51	P40559	3	EBI-25970,EBI-24915

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 604	604	Protein TAX4		PRO_0000203050
Regions
	Domain	469 – 559	91	EH
	Compositional bias	182 – 239	58	Ser-rich
	Compositional bias	397 – 422	26	His-rich

Sequences

	Sequence		Length	Mass (Da)
	P47030 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: E17C6C71D9FBFBD6	FASTA	604	68,768
	10 20 30 40 50 60 MHFPKKKHSG NLSVVELPKE ALQDSLTAAQ ITFKRYAHPN GNAGSAERPR HLKVESAPVV 70 80 90 100 110 120 KSEPSLPRMR QPEPRSINHQ YSRETLPGHS EAFSVPTTPL QTIHYDVRNK ASNSPSSIAA 130 140 150 160 170 180 AETAAYLAHT NSFSNRSSGV GSRDPVMDTE TKPPRAPSAL KNELQLNRMR IPPPSYDNNV 190 200 210 220 230 240 RSRSISPQVS YSTSLSSSCS ISSDGEETSY REKSTDEAFP PEPSMSSYSL ASKASAKASL 250 260 270 280 290 300 TDPSQRQQES DYTAMNKLNG GNIIYKGTLP DLIPRSQRKT SKPRFKHRLL RSPEQQQENL 310 320 330 340 350 360 SRVYSDQTQN GRAIINTQQN VKLKTTMRRG KYAITDNDET FPYDRKSVSS DSDTDEDSNV 370 380 390 400 410 420 MEIKDKKKKS RRSKIKKGLK TTAAVVGSST SVLPFPHHHH HHHQLHNPNS HHLHTHHHTS 430 440 450 460 470 480 SHKFNEDKPW KSHRDLGFIT EQERKRYESM WVSNRYSYLR LLPWWPSLAN EDDESHLQPL 490 500 510 520 530 540 NLPQDGLMLN LVVKDIWYRS NLPRDLLVQI YNMVDTRKDG TLDRKSFIVG MWLVDQCLYG 550 560 570 580 590 600 RKLTNELDQR VWNSVDGYVL GTINVKPATS DHYHNANNPL DKPSKLSVRQ ELKNIKRDLR NVRI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of a 33.1 kb fragment from the left arm of Saccharomyces cerevisiae chromosome X, including putative proteins with leucine zippers, a fungal Zn(II)2-Cys6 binuclear cluster domain and a putative alpha 2-SCB-alpha 2 binding site." Miosga T., Schaaff-Gerstenschlaeger I., Chalwatzis N., Baur A., Boles E., Fournier C., Schmitt S., Velten C., Wilhelm N., Zimmermann F.K. Yeast 11:681-689(1995) [PubMed: 7483841] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Recognition specificity of individual EH domains of mammals and yeast." Paoluzi S., Castagnoli L., Lauro I., Salcini A.E., Coda L., Fre' S., Confalonieri S., Pelicci P.G., Di Fiore P.P., Cesareni G. EMBO J. 17:6541-6550(1998) [PubMed: 9822599] [Abstract] Cited for: DOMAIN.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Negative regulation of phosphatidylinositol 4,5-bisphosphate levels by the INP51-associated proteins TAX4 and IRS4." Morales-Johansson H., Jenoe P., Cooke F.T., Hall M.N. J. Biol. Chem. 279:39604-39610(2004) [PubMed: 15265867] [Abstract] Cited for: FUNCTION, INTERACTION WITH INP51.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z49358 Genomic DNA. Translation: CAA89376.1. X83502 Genomic DNA. Translation: CAA58487.1. X88851 Genomic DNA. Translation: CAA61318.1. BK006943 Genomic DNA. Translation: DAA08716.1.
PIR	S56027.
RefSeq	NP_012452.1. NM_001181516.1.
3D structure databases
ProteinModelPortal	P47030.
SMR	P47030. Positions 435-554.
ModBase	Search...
Protein-protein interaction databases
IntAct	P47030. 5 interactions.
MINT	MINT-598564.
STRING	P47030.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJL083W; YJL083W; YJL083W.
GeneID	853362.
KEGG	sce:YJL083W.
NMPDR	fig|4932.3.peg.3421.
Organism-specific databases
CYGD	YJL083w.
SGD	S000003619. TAX4.
Phylogenomic databases
eggNOG	fuNOG12147.
GeneTree	EFGT00050000001551.
OrthoDB	EOG4X3M8X.
Gene expression databases
ArrayExpress	P47030.
Genevestigator	P47030.
GermOnline	YJL083W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011992. EF-hand-like_dom. IPR000261. EPS15_homology. [Graphical view]
Gene3D	G3DSA:1.10.238.10. EF-Hand_type. 1 hit.
SMART	SM00027. EH. 1 hit. [Graphical view]
PROSITE	PS50031. EH. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	973786.

Entry information

Entry name

TAX4_YEAST

Accession

Primary (citable) accession number: P47030
Secondary accession number(s): D6VWA0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	December 14, 2011
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program