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P47029

- ALY2_YEAST

UniProt

P47029 - ALY2_YEAST

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Protein
Arrestin-related trafficking adapter 3
Gene
ALY2, ART3, YJL084C, J0934
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

May regulate endocytosis by recruiting RSP5 ubiquitin ligase activity to specific plasma membrane proteins in response to extracellular stimuli.1 Publication

GO - Molecular functioni

  1. protein binding Source: IntAct
  2. protein binding, bridging Source: SGD
  3. ubiquitin protein ligase binding Source: SGD

GO - Biological processi

  1. positive regulation of ubiquitin-dependent endocytosis Source: SGD
  2. regulation of intracellular transport Source: SGD
  3. ubiquitin-dependent endocytosis Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-31541-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Arrestin-related trafficking adapter 3
Alternative name(s):
Arrestin-like protein 2
Gene namesi
Name:ALY2
Synonyms:ART3
Ordered Locus Names:YJL084C
ORF Names:J0934
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

CYGDiYJL084c.
SGDiS000003620. ALY2.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. early endosome Source: SGD
  2. late endosome Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10461046Arrestin-related trafficking adapter 3
PRO_0000203049Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei155 – 1551Phosphoserine3 Publications
Modified residuei162 – 1621Phosphoserine1 Publication
Modified residuei213 – 2131Phosphoserine1 Publication
Modified residuei586 – 5861Phosphoserine1 Publication
Modified residuei826 – 8261Phosphoserine1 Publication
Modified residuei838 – 8381Phosphoserine3 Publications
Modified residuei900 – 9001Phosphoserine2 Publications
Modified residuei1022 – 10221Phosphoserine1 Publication
Modified residuei1023 – 10231Phosphoserine1 Publication

Post-translational modificationi

Ubiquitinated by RSP5.1 Publication
Phosphorylated by the cyclin-CDKs PCL6-PHO85 and PCL7-PHO85.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP47029.
PaxDbiP47029.
PeptideAtlasiP47029.

Expressioni

Gene expression databases

GenevestigatoriP47029.

Interactioni

Subunit structurei

Interacts with PCL6, PCL7 and RSP5.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RSP5P399405EBI-25974,EBI-16219

Protein-protein interaction databases

BioGridi33672. 62 interactions.
DIPiDIP-1497N.
IntActiP47029. 9 interactions.
MINTiMINT-394519.
STRINGi4932.YJL084C.

Structurei

3D structure databases

ProteinModelPortaliP47029.

Family & Domainsi

Sequence similaritiesi

Belongs to the ALY1 family.

Phylogenomic databases

eggNOGiNOG292343.
GeneTreeiENSGT00730000113838.
OMAiHRIRIYV.
OrthoDBiEOG7P2Z1W.

Family and domain databases

InterProiIPR011021. Arrestin-like_N.
IPR011022. Arrestin_C-like.
IPR014756. Ig_E-set.
[Graphical view]
PfamiPF02752. Arrestin_C. 1 hit.
PF00339. Arrestin_N. 1 hit.
[Graphical view]
SMARTiSM01017. Arrestin_C. 1 hit.
[Graphical view]
SUPFAMiSSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

P47029-1 [UniParc]FASTAAdd to Basket

« Hide

MPMDQSISSP LFPMEKDIDI PLDATPLAQS SSLQLFIHLA EPVVFLQGFD     50
PQKTEYPSVV LRGCLVVRIL KPTKLKSISL SFKGYSRTEW PEGIPPKRQE 100
FVEIKDIVDH TWALYPPTEQ KSKKKMDASA PNESNNAANN FLTKESGASL 150
YRTLSDNETI TSRKNSISGL SSLNLSPLGA PGNSSVNVKD RESRQRSRSS 200
SVTSSNGPSR NLSPINLLKR ATSPSVSHHN YKPTTTSIFS DLLNNTFTHN 250
DAASHHGHHI PTSSNHLAMT SNNFTSGSGG EFFVFQPGDY IYAFEELIPQ 300
AYPESIKADF GFVEYFLFAS IERPGAFKSN ISARQVVNIV RTQAHNSVEE 350
SEPIIISRDW ENQLYYDIVI ASKDIILDAF LPITFKFAPL DKVTLHRIRI 400
YVTETMEYYC REKKVHRMEP TKKFLLTEQK GPKLPNLPND ANLSKAKNMG 450
NLLQDPKNGD LVNKEYEYQI FIPSRFNNHQ QLHPDTSYEN IKANHWIKIC 500
LRLSRVVDNK RKHYEISIDS PIHVLHRLCS HANTLLPSYD GHPASFPKET 550
DSSISSILES SDDNINLYHN SNIFFPKEVL SSPVLSPNVQ PLDILIPHLP 600
STSLTRNSRQ FNRNSKSHPS DNTIFNSAKL KSNIYQPESL QRELASPQAI 650
PLSPITSPMS NMEVPPPDFD FSSDFISDAA SGTTTTEVSS SESSILPRDP 700
PSYKDTVLHD NNQKRRPNSK HPTPPSLKAS HPNKNSDKNS SETLNKKESM 750
SKIEENKHKR ETTPKKRENR DVKSLSTPQR EESKDSTSTG NQSNEKNRKR 800
VLSLSSSLHS SPNNSGFAHS ALGNLSNESL RSLNRRESVQ DNLPSTIRHD 850
NPFFTDLNQV LIEDELKNHD KNELNRHSTN TSSTPASARS SFDYSGINIS 900
KDKLNMEPLL SKTETLTNKV NEDSFLRPND SYVDLLEPSV DTTIDITAPY 950
ARNSSAWHPL QNDNDNNQFS PLLGSNENFL NAANAQNSAE SDHNNDIFTQ 1000
GSGLTESSKN SDSEERFISR LSSPEKVLIN TLDNESGLQS INESTL 1046
Length:1,046
Mass (Da):117,216
Last modified:November 1, 1997 - v2
Checksum:iEAC5711EE1AB1C66
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti852 – 8521P → G in CAA58486. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X83502 Genomic DNA. Translation: CAA58486.1.
Z49359 Genomic DNA. Translation: CAA89377.1.
X88851 Genomic DNA. Translation: CAA61319.1.
BK006943 Genomic DNA. Translation: DAA08715.1.
PIRiS56026.
RefSeqiNP_012451.1. NM_001181517.1.

Genome annotation databases

EnsemblFungiiYJL084C; YJL084C; YJL084C.
GeneIDi853361.
KEGGisce:YJL084C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X83502 Genomic DNA. Translation: CAA58486.1 .
Z49359 Genomic DNA. Translation: CAA89377.1 .
X88851 Genomic DNA. Translation: CAA61319.1 .
BK006943 Genomic DNA. Translation: DAA08715.1 .
PIRi S56026.
RefSeqi NP_012451.1. NM_001181517.1.

3D structure databases

ProteinModelPortali P47029.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33672. 62 interactions.
DIPi DIP-1497N.
IntActi P47029. 9 interactions.
MINTi MINT-394519.
STRINGi 4932.YJL084C.

Proteomic databases

MaxQBi P47029.
PaxDbi P47029.
PeptideAtlasi P47029.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YJL084C ; YJL084C ; YJL084C .
GeneIDi 853361.
KEGGi sce:YJL084C.

Organism-specific databases

CYGDi YJL084c.
SGDi S000003620. ALY2.

Phylogenomic databases

eggNOGi NOG292343.
GeneTreei ENSGT00730000113838.
OMAi HRIRIYV.
OrthoDBi EOG7P2Z1W.

Enzyme and pathway databases

BioCyci YEAST:G3O-31541-MONOMER.

Miscellaneous databases

NextBioi 973783.

Gene expression databases

Genevestigatori P47029.

Family and domain databases

InterProi IPR011021. Arrestin-like_N.
IPR011022. Arrestin_C-like.
IPR014756. Ig_E-set.
[Graphical view ]
Pfami PF02752. Arrestin_C. 1 hit.
PF00339. Arrestin_N. 1 hit.
[Graphical view ]
SMARTi SM01017. Arrestin_C. 1 hit.
[Graphical view ]
SUPFAMi SSF81296. SSF81296. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of a 33.1 kb fragment from the left arm of Saccharomyces cerevisiae chromosome X, including putative proteins with leucine zippers, a fungal Zn(II)2-Cys6 binuclear cluster domain and a putative alpha 2-SCB-alpha 2 binding site."
    Miosga T., Schaaff-Gerstenschlaeger I., Chalwatzis N., Baur A., Boles E., Fournier C., Schmitt S., Velten C., Wilhelm N., Zimmermann F.K.
    Yeast 11:681-689(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Analysis of phosphorylation of YJL084c, a yeast protein."
    Shi X.Z., Ao S.Z.
    Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao 34:433-438(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION, INTERACTION WITH PCL6 AND PCL7.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
    Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
    Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-213, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: YAL6B.
  8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-155; SER-162 AND SER-838, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  9. "Ubiquitination screen using protein microarrays for comprehensive identification of Rsp5 substrates in yeast."
    Gupta R., Kus B., Fladd C., Wasmuth J., Tonikian R., Sidhu S., Krogan N.J., Parkinson J., Rotin D.
    Mol. Syst. Biol. 3:116-116(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RSP5, UBIQUITINATION BY RSP5.
  10. "Arrestin-related ubiquitin-ligase adaptors regulate endocytosis and protein turnover at the cell surface."
    Lin C.H., MacGurn J.A., Chu T., Stefan C.J., Emr S.D.
    Cell 135:714-725(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RPS5, FUNCTION.
  11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-155; SER-826; SER-838 AND SER-900, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-155; SER-586; SER-838; SER-900; SER-1022 AND SER-1023, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiALY2_YEAST
AccessioniPrimary (citable) accession number: P47029
Secondary accession number(s): D6VW99, Q05742
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: November 1, 1997
Last modified: June 11, 2014
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 49 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3

Similar proteinsi