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Protein

Arrestin-related trafficking adapter 3

Gene

ALY2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May regulate endocytosis by recruiting RSP5 ubiquitin ligase activity to specific plasma membrane proteins in response to extracellular stimuli.1 Publication

GO - Molecular functioni

  1. protein binding, bridging Source: SGD
  2. ubiquitin protein ligase binding Source: SGD

GO - Biological processi

  1. positive regulation of ubiquitin-dependent endocytosis Source: SGD
  2. regulation of intracellular transport Source: SGD
  3. ubiquitin-dependent endocytosis Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-31541-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Arrestin-related trafficking adapter 3
Alternative name(s):
Arrestin-like protein 2
Gene namesi
Name:ALY2
Synonyms:ART3
Ordered Locus Names:YJL084C
ORF Names:J0934
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

CYGDiYJL084c.
SGDiS000003620. ALY2.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. early endosome Source: SGD
  2. late endosome Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10461046Arrestin-related trafficking adapter 3PRO_0000203049Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei155 – 1551Phosphoserine3 Publications
Modified residuei162 – 1621Phosphoserine1 Publication
Modified residuei213 – 2131Phosphoserine1 Publication
Modified residuei586 – 5861Phosphoserine1 Publication
Modified residuei826 – 8261Phosphoserine1 Publication
Modified residuei838 – 8381Phosphoserine3 Publications
Modified residuei900 – 9001Phosphoserine2 Publications
Modified residuei1022 – 10221Phosphoserine1 Publication
Modified residuei1023 – 10231Phosphoserine1 Publication

Post-translational modificationi

Ubiquitinated by RSP5.1 Publication
Phosphorylated by the cyclin-CDKs PCL6-PHO85 and PCL7-PHO85.5 Publications

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP47029.
PaxDbiP47029.
PeptideAtlasiP47029.

Expressioni

Gene expression databases

GenevestigatoriP47029.

Interactioni

Subunit structurei

Interacts with PCL6, PCL7 and RSP5.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RSP5P399405EBI-25974,EBI-16219

Protein-protein interaction databases

BioGridi33672. 62 interactions.
DIPiDIP-1497N.
IntActiP47029. 9 interactions.
MINTiMINT-394519.
STRINGi4932.YJL084C.

Structurei

3D structure databases

ProteinModelPortaliP47029.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ALY1 family.Curated

Phylogenomic databases

eggNOGiNOG292343.
GeneTreeiENSGT00730000113838.
InParanoidiP47029.
OMAiHRIRIYV.
OrthoDBiEOG7P2Z1W.

Family and domain databases

InterProiIPR011021. Arrestin-like_N.
IPR011022. Arrestin_C-like.
IPR014756. Ig_E-set.
[Graphical view]
PfamiPF02752. Arrestin_C. 1 hit.
PF00339. Arrestin_N. 1 hit.
[Graphical view]
SMARTiSM01017. Arrestin_C. 1 hit.
[Graphical view]
SUPFAMiSSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

P47029-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPMDQSISSP LFPMEKDIDI PLDATPLAQS SSLQLFIHLA EPVVFLQGFD
60 70 80 90 100
PQKTEYPSVV LRGCLVVRIL KPTKLKSISL SFKGYSRTEW PEGIPPKRQE
110 120 130 140 150
FVEIKDIVDH TWALYPPTEQ KSKKKMDASA PNESNNAANN FLTKESGASL
160 170 180 190 200
YRTLSDNETI TSRKNSISGL SSLNLSPLGA PGNSSVNVKD RESRQRSRSS
210 220 230 240 250
SVTSSNGPSR NLSPINLLKR ATSPSVSHHN YKPTTTSIFS DLLNNTFTHN
260 270 280 290 300
DAASHHGHHI PTSSNHLAMT SNNFTSGSGG EFFVFQPGDY IYAFEELIPQ
310 320 330 340 350
AYPESIKADF GFVEYFLFAS IERPGAFKSN ISARQVVNIV RTQAHNSVEE
360 370 380 390 400
SEPIIISRDW ENQLYYDIVI ASKDIILDAF LPITFKFAPL DKVTLHRIRI
410 420 430 440 450
YVTETMEYYC REKKVHRMEP TKKFLLTEQK GPKLPNLPND ANLSKAKNMG
460 470 480 490 500
NLLQDPKNGD LVNKEYEYQI FIPSRFNNHQ QLHPDTSYEN IKANHWIKIC
510 520 530 540 550
LRLSRVVDNK RKHYEISIDS PIHVLHRLCS HANTLLPSYD GHPASFPKET
560 570 580 590 600
DSSISSILES SDDNINLYHN SNIFFPKEVL SSPVLSPNVQ PLDILIPHLP
610 620 630 640 650
STSLTRNSRQ FNRNSKSHPS DNTIFNSAKL KSNIYQPESL QRELASPQAI
660 670 680 690 700
PLSPITSPMS NMEVPPPDFD FSSDFISDAA SGTTTTEVSS SESSILPRDP
710 720 730 740 750
PSYKDTVLHD NNQKRRPNSK HPTPPSLKAS HPNKNSDKNS SETLNKKESM
760 770 780 790 800
SKIEENKHKR ETTPKKRENR DVKSLSTPQR EESKDSTSTG NQSNEKNRKR
810 820 830 840 850
VLSLSSSLHS SPNNSGFAHS ALGNLSNESL RSLNRRESVQ DNLPSTIRHD
860 870 880 890 900
NPFFTDLNQV LIEDELKNHD KNELNRHSTN TSSTPASARS SFDYSGINIS
910 920 930 940 950
KDKLNMEPLL SKTETLTNKV NEDSFLRPND SYVDLLEPSV DTTIDITAPY
960 970 980 990 1000
ARNSSAWHPL QNDNDNNQFS PLLGSNENFL NAANAQNSAE SDHNNDIFTQ
1010 1020 1030 1040
GSGLTESSKN SDSEERFISR LSSPEKVLIN TLDNESGLQS INESTL
Length:1,046
Mass (Da):117,216
Last modified:November 1, 1997 - v2
Checksum:iEAC5711EE1AB1C66
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti852 – 8521P → G in CAA58486. (PubMed:7483841)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83502 Genomic DNA. Translation: CAA58486.1.
Z49359 Genomic DNA. Translation: CAA89377.1.
X88851 Genomic DNA. Translation: CAA61319.1.
BK006943 Genomic DNA. Translation: DAA08715.1.
PIRiS56026.
RefSeqiNP_012451.1. NM_001181517.1.

Genome annotation databases

EnsemblFungiiYJL084C; YJL084C; YJL084C.
GeneIDi853361.
KEGGisce:YJL084C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83502 Genomic DNA. Translation: CAA58486.1.
Z49359 Genomic DNA. Translation: CAA89377.1.
X88851 Genomic DNA. Translation: CAA61319.1.
BK006943 Genomic DNA. Translation: DAA08715.1.
PIRiS56026.
RefSeqiNP_012451.1. NM_001181517.1.

3D structure databases

ProteinModelPortaliP47029.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33672. 62 interactions.
DIPiDIP-1497N.
IntActiP47029. 9 interactions.
MINTiMINT-394519.
STRINGi4932.YJL084C.

Proteomic databases

MaxQBiP47029.
PaxDbiP47029.
PeptideAtlasiP47029.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJL084C; YJL084C; YJL084C.
GeneIDi853361.
KEGGisce:YJL084C.

Organism-specific databases

CYGDiYJL084c.
SGDiS000003620. ALY2.

Phylogenomic databases

eggNOGiNOG292343.
GeneTreeiENSGT00730000113838.
InParanoidiP47029.
OMAiHRIRIYV.
OrthoDBiEOG7P2Z1W.

Enzyme and pathway databases

BioCyciYEAST:G3O-31541-MONOMER.

Miscellaneous databases

NextBioi973783.

Gene expression databases

GenevestigatoriP47029.

Family and domain databases

InterProiIPR011021. Arrestin-like_N.
IPR011022. Arrestin_C-like.
IPR014756. Ig_E-set.
[Graphical view]
PfamiPF02752. Arrestin_C. 1 hit.
PF00339. Arrestin_N. 1 hit.
[Graphical view]
SMARTiSM01017. Arrestin_C. 1 hit.
[Graphical view]
SUPFAMiSSF81296. SSF81296. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of a 33.1 kb fragment from the left arm of Saccharomyces cerevisiae chromosome X, including putative proteins with leucine zippers, a fungal Zn(II)2-Cys6 binuclear cluster domain and a putative alpha 2-SCB-alpha 2 binding site."
    Miosga T., Schaaff-Gerstenschlaeger I., Chalwatzis N., Baur A., Boles E., Fournier C., Schmitt S., Velten C., Wilhelm N., Zimmermann F.K.
    Yeast 11:681-689(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Analysis of phosphorylation of YJL084c, a yeast protein."
    Shi X.Z., Ao S.Z.
    Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao 34:433-438(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION, INTERACTION WITH PCL6 AND PCL7.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
    Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
    Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-213, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: YAL6B.
  8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-155; SER-162 AND SER-838, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  9. "Ubiquitination screen using protein microarrays for comprehensive identification of Rsp5 substrates in yeast."
    Gupta R., Kus B., Fladd C., Wasmuth J., Tonikian R., Sidhu S., Krogan N.J., Parkinson J., Rotin D.
    Mol. Syst. Biol. 3:116-116(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RSP5, UBIQUITINATION BY RSP5.
  10. "Arrestin-related ubiquitin-ligase adaptors regulate endocytosis and protein turnover at the cell surface."
    Lin C.H., MacGurn J.A., Chu T., Stefan C.J., Emr S.D.
    Cell 135:714-725(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RPS5, FUNCTION.
  11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-155; SER-826; SER-838 AND SER-900, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-155; SER-586; SER-838; SER-900; SER-1022 AND SER-1023, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiALY2_YEAST
AccessioniPrimary (citable) accession number: P47029
Secondary accession number(s): D6VW99, Q05742
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: November 1, 1997
Last modified: January 7, 2015
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 49 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.