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P47029 (ALY2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Arrestin-related trafficking adapter 3
Alternative name(s):
Arrestin-like protein 2
Gene names
Name:ALY2
Synonyms:ART3
Ordered Locus Names:YJL084C
ORF Names:J0934
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1046 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May regulate endocytosis by recruiting RSP5 ubiquitin ligase activity to specific plasma membrane proteins in response to extracellular stimuli. Ref.10

Subunit structure

Interacts with PCL6, PCL7 and RSP5. Ref.4 Ref.9 Ref.10

Subcellular location

Cytoplasm Ref.5.

Post-translational modification

Ubiquitinated by RSP5. Ref.9

Phosphorylated by the cyclin-CDKs PCL6-PHO85 and PCL7-PHO85. Ref.4

Miscellaneous

Present with 49 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the ALY1 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

RSP5P399405EBI-25974,EBI-16219

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10461046Arrestin-related trafficking adapter 3
PRO_0000203049

Amino acid modifications

Modified residue1551Phosphoserine Ref.8 Ref.11 Ref.12
Modified residue1621Phosphoserine Ref.8
Modified residue2131Phosphoserine Ref.7
Modified residue5861Phosphoserine Ref.12
Modified residue8261Phosphoserine Ref.11
Modified residue8381Phosphoserine Ref.8 Ref.11 Ref.12
Modified residue9001Phosphoserine Ref.11 Ref.12
Modified residue10221Phosphoserine Ref.12
Modified residue10231Phosphoserine Ref.12

Experimental info

Sequence conflict8521P → G in CAA58486. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P47029 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: EAC5711EE1AB1C66

FASTA1,046117,216
        10         20         30         40         50         60 
MPMDQSISSP LFPMEKDIDI PLDATPLAQS SSLQLFIHLA EPVVFLQGFD PQKTEYPSVV 

        70         80         90        100        110        120 
LRGCLVVRIL KPTKLKSISL SFKGYSRTEW PEGIPPKRQE FVEIKDIVDH TWALYPPTEQ 

       130        140        150        160        170        180 
KSKKKMDASA PNESNNAANN FLTKESGASL YRTLSDNETI TSRKNSISGL SSLNLSPLGA 

       190        200        210        220        230        240 
PGNSSVNVKD RESRQRSRSS SVTSSNGPSR NLSPINLLKR ATSPSVSHHN YKPTTTSIFS 

       250        260        270        280        290        300 
DLLNNTFTHN DAASHHGHHI PTSSNHLAMT SNNFTSGSGG EFFVFQPGDY IYAFEELIPQ 

       310        320        330        340        350        360 
AYPESIKADF GFVEYFLFAS IERPGAFKSN ISARQVVNIV RTQAHNSVEE SEPIIISRDW 

       370        380        390        400        410        420 
ENQLYYDIVI ASKDIILDAF LPITFKFAPL DKVTLHRIRI YVTETMEYYC REKKVHRMEP 

       430        440        450        460        470        480 
TKKFLLTEQK GPKLPNLPND ANLSKAKNMG NLLQDPKNGD LVNKEYEYQI FIPSRFNNHQ 

       490        500        510        520        530        540 
QLHPDTSYEN IKANHWIKIC LRLSRVVDNK RKHYEISIDS PIHVLHRLCS HANTLLPSYD 

       550        560        570        580        590        600 
GHPASFPKET DSSISSILES SDDNINLYHN SNIFFPKEVL SSPVLSPNVQ PLDILIPHLP 

       610        620        630        640        650        660 
STSLTRNSRQ FNRNSKSHPS DNTIFNSAKL KSNIYQPESL QRELASPQAI PLSPITSPMS 

       670        680        690        700        710        720 
NMEVPPPDFD FSSDFISDAA SGTTTTEVSS SESSILPRDP PSYKDTVLHD NNQKRRPNSK 

       730        740        750        760        770        780 
HPTPPSLKAS HPNKNSDKNS SETLNKKESM SKIEENKHKR ETTPKKRENR DVKSLSTPQR 

       790        800        810        820        830        840 
EESKDSTSTG NQSNEKNRKR VLSLSSSLHS SPNNSGFAHS ALGNLSNESL RSLNRRESVQ 

       850        860        870        880        890        900 
DNLPSTIRHD NPFFTDLNQV LIEDELKNHD KNELNRHSTN TSSTPASARS SFDYSGINIS 

       910        920        930        940        950        960 
KDKLNMEPLL SKTETLTNKV NEDSFLRPND SYVDLLEPSV DTTIDITAPY ARNSSAWHPL 

       970        980        990       1000       1010       1020 
QNDNDNNQFS PLLGSNENFL NAANAQNSAE SDHNNDIFTQ GSGLTESSKN SDSEERFISR 

      1030       1040 
LSSPEKVLIN TLDNESGLQS INESTL 

« Hide

References

« Hide 'large scale' references
[1]"Sequence analysis of a 33.1 kb fragment from the left arm of Saccharomyces cerevisiae chromosome X, including putative proteins with leucine zippers, a fungal Zn(II)2-Cys6 binuclear cluster domain and a putative alpha 2-SCB-alpha 2 binding site."
Miosga T., Schaaff-Gerstenschlaeger I., Chalwatzis N., Baur A., Boles E., Fournier C., Schmitt S., Velten C., Wilhelm N., Zimmermann F.K.
Yeast 11:681-689(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Analysis of phosphorylation of YJL084c, a yeast protein."
Shi X.Z., Ao S.Z.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao 34:433-438(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION, INTERACTION WITH PCL6 AND PCL7.
[5]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-213, MASS SPECTROMETRY, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: YAL6B.
[8]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-155; SER-162 AND SER-838, MASS SPECTROMETRY, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[9]"Ubiquitination screen using protein microarrays for comprehensive identification of Rsp5 substrates in yeast."
Gupta R., Kus B., Fladd C., Wasmuth J., Tonikian R., Sidhu S., Krogan N.J., Parkinson J., Rotin D.
Mol. Syst. Biol. 3:116-116(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RSP5, UBIQUITINATION BY RSP5.
[10]"Arrestin-related ubiquitin-ligase adaptors regulate endocytosis and protein turnover at the cell surface."
Lin C.H., MacGurn J.A., Chu T., Stefan C.J., Emr S.D.
Cell 135:714-725(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RPS5, FUNCTION.
[11]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-155; SER-826; SER-838 AND SER-900, MASS SPECTROMETRY, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-155; SER-586; SER-838; SER-900; SER-1022 AND SER-1023, MASS SPECTROMETRY, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X83502 Genomic DNA. Translation: CAA58486.1.
Z49359 Genomic DNA. Translation: CAA89377.1.
X88851 Genomic DNA. Translation: CAA61319.1.
BK006943 Genomic DNA. Translation: DAA08715.1.
PIRS56026.
RefSeqNP_012451.1. NM_001181517.1.

3D structure databases

ProteinModelPortalP47029.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33672. 62 interactions.
DIPDIP-1497N.
IntActP47029. 9 interactions.
MINTMINT-394519.
STRING4932.YJL084C.

Proteomic databases

PaxDbP47029.
PeptideAtlasP47029.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJL084C; YJL084C; YJL084C.
GeneID853361.
KEGGsce:YJL084C.

Organism-specific databases

CYGDYJL084c.
SGDS000003620. ALY2.

Phylogenomic databases

eggNOGNOG292343.
GeneTreeENSGT00730000113838.
OMAHRIRIYV.
OrthoDBEOG7P2Z1W.

Enzyme and pathway databases

BioCycYEAST:G3O-31541-MONOMER.

Gene expression databases

GenevestigatorP47029.

Family and domain databases

InterProIPR011021. Arrestin-like_N.
IPR011022. Arrestin_C-like.
IPR014756. Ig_E-set.
[Graphical view]
PfamPF02752. Arrestin_C. 1 hit.
PF00339. Arrestin_N. 1 hit.
[Graphical view]
SMARTSM01017. Arrestin_C. 1 hit.
[Graphical view]
SUPFAMSSF81296. SSF81296. 1 hit.
ProtoNetSearch...

Other

NextBio973783.

Entry information

Entry nameALY2_YEAST
AccessionPrimary (citable) accession number: P47029
Secondary accession number(s): D6VW99, Q05742
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: November 1, 1997
Last modified: March 19, 2014
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families