Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P47029

- ALY2_YEAST

UniProt

P47029 - ALY2_YEAST

Protein

Arrestin-related trafficking adapter 3

Gene

ALY2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 2 (01 Nov 1997)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    May regulate endocytosis by recruiting RSP5 ubiquitin ligase activity to specific plasma membrane proteins in response to extracellular stimuli.1 Publication

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. protein binding, bridging Source: SGD
    3. ubiquitin protein ligase binding Source: SGD

    GO - Biological processi

    1. positive regulation of ubiquitin-dependent endocytosis Source: SGD
    2. regulation of intracellular transport Source: SGD
    3. ubiquitin-dependent endocytosis Source: SGD

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31541-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Arrestin-related trafficking adapter 3
    Alternative name(s):
    Arrestin-like protein 2
    Gene namesi
    Name:ALY2
    Synonyms:ART3
    Ordered Locus Names:YJL084C
    ORF Names:J0934
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome X

    Organism-specific databases

    CYGDiYJL084c.
    SGDiS000003620. ALY2.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. early endosome Source: SGD
    2. late endosome Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10461046Arrestin-related trafficking adapter 3PRO_0000203049Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei155 – 1551Phosphoserine4 Publications
    Modified residuei162 – 1621Phosphoserine2 Publications
    Modified residuei213 – 2131Phosphoserine2 Publications
    Modified residuei586 – 5861Phosphoserine2 Publications
    Modified residuei826 – 8261Phosphoserine2 Publications
    Modified residuei838 – 8381Phosphoserine4 Publications
    Modified residuei900 – 9001Phosphoserine3 Publications
    Modified residuei1022 – 10221Phosphoserine2 Publications
    Modified residuei1023 – 10231Phosphoserine2 Publications

    Post-translational modificationi

    Ubiquitinated by RSP5.1 Publication
    Phosphorylated by the cyclin-CDKs PCL6-PHO85 and PCL7-PHO85.5 Publications

    Keywords - PTMi

    Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiP47029.
    PaxDbiP47029.
    PeptideAtlasiP47029.

    Expressioni

    Gene expression databases

    GenevestigatoriP47029.

    Interactioni

    Subunit structurei

    Interacts with PCL6, PCL7 and RSP5.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    RSP5P399405EBI-25974,EBI-16219

    Protein-protein interaction databases

    BioGridi33672. 62 interactions.
    DIPiDIP-1497N.
    IntActiP47029. 9 interactions.
    MINTiMINT-394519.
    STRINGi4932.YJL084C.

    Structurei

    3D structure databases

    ProteinModelPortaliP47029.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ALY1 family.Curated

    Phylogenomic databases

    eggNOGiNOG292343.
    GeneTreeiENSGT00730000113838.
    OMAiHRIRIYV.
    OrthoDBiEOG7P2Z1W.

    Family and domain databases

    InterProiIPR011021. Arrestin-like_N.
    IPR011022. Arrestin_C-like.
    IPR014756. Ig_E-set.
    [Graphical view]
    PfamiPF02752. Arrestin_C. 1 hit.
    PF00339. Arrestin_N. 1 hit.
    [Graphical view]
    SMARTiSM01017. Arrestin_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF81296. SSF81296. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P47029-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPMDQSISSP LFPMEKDIDI PLDATPLAQS SSLQLFIHLA EPVVFLQGFD     50
    PQKTEYPSVV LRGCLVVRIL KPTKLKSISL SFKGYSRTEW PEGIPPKRQE 100
    FVEIKDIVDH TWALYPPTEQ KSKKKMDASA PNESNNAANN FLTKESGASL 150
    YRTLSDNETI TSRKNSISGL SSLNLSPLGA PGNSSVNVKD RESRQRSRSS 200
    SVTSSNGPSR NLSPINLLKR ATSPSVSHHN YKPTTTSIFS DLLNNTFTHN 250
    DAASHHGHHI PTSSNHLAMT SNNFTSGSGG EFFVFQPGDY IYAFEELIPQ 300
    AYPESIKADF GFVEYFLFAS IERPGAFKSN ISARQVVNIV RTQAHNSVEE 350
    SEPIIISRDW ENQLYYDIVI ASKDIILDAF LPITFKFAPL DKVTLHRIRI 400
    YVTETMEYYC REKKVHRMEP TKKFLLTEQK GPKLPNLPND ANLSKAKNMG 450
    NLLQDPKNGD LVNKEYEYQI FIPSRFNNHQ QLHPDTSYEN IKANHWIKIC 500
    LRLSRVVDNK RKHYEISIDS PIHVLHRLCS HANTLLPSYD GHPASFPKET 550
    DSSISSILES SDDNINLYHN SNIFFPKEVL SSPVLSPNVQ PLDILIPHLP 600
    STSLTRNSRQ FNRNSKSHPS DNTIFNSAKL KSNIYQPESL QRELASPQAI 650
    PLSPITSPMS NMEVPPPDFD FSSDFISDAA SGTTTTEVSS SESSILPRDP 700
    PSYKDTVLHD NNQKRRPNSK HPTPPSLKAS HPNKNSDKNS SETLNKKESM 750
    SKIEENKHKR ETTPKKRENR DVKSLSTPQR EESKDSTSTG NQSNEKNRKR 800
    VLSLSSSLHS SPNNSGFAHS ALGNLSNESL RSLNRRESVQ DNLPSTIRHD 850
    NPFFTDLNQV LIEDELKNHD KNELNRHSTN TSSTPASARS SFDYSGINIS 900
    KDKLNMEPLL SKTETLTNKV NEDSFLRPND SYVDLLEPSV DTTIDITAPY 950
    ARNSSAWHPL QNDNDNNQFS PLLGSNENFL NAANAQNSAE SDHNNDIFTQ 1000
    GSGLTESSKN SDSEERFISR LSSPEKVLIN TLDNESGLQS INESTL 1046
    Length:1,046
    Mass (Da):117,216
    Last modified:November 1, 1997 - v2
    Checksum:iEAC5711EE1AB1C66
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti852 – 8521P → G in CAA58486. (PubMed:7483841)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X83502 Genomic DNA. Translation: CAA58486.1.
    Z49359 Genomic DNA. Translation: CAA89377.1.
    X88851 Genomic DNA. Translation: CAA61319.1.
    BK006943 Genomic DNA. Translation: DAA08715.1.
    PIRiS56026.
    RefSeqiNP_012451.1. NM_001181517.1.

    Genome annotation databases

    EnsemblFungiiYJL084C; YJL084C; YJL084C.
    GeneIDi853361.
    KEGGisce:YJL084C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X83502 Genomic DNA. Translation: CAA58486.1 .
    Z49359 Genomic DNA. Translation: CAA89377.1 .
    X88851 Genomic DNA. Translation: CAA61319.1 .
    BK006943 Genomic DNA. Translation: DAA08715.1 .
    PIRi S56026.
    RefSeqi NP_012451.1. NM_001181517.1.

    3D structure databases

    ProteinModelPortali P47029.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33672. 62 interactions.
    DIPi DIP-1497N.
    IntActi P47029. 9 interactions.
    MINTi MINT-394519.
    STRINGi 4932.YJL084C.

    Proteomic databases

    MaxQBi P47029.
    PaxDbi P47029.
    PeptideAtlasi P47029.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YJL084C ; YJL084C ; YJL084C .
    GeneIDi 853361.
    KEGGi sce:YJL084C.

    Organism-specific databases

    CYGDi YJL084c.
    SGDi S000003620. ALY2.

    Phylogenomic databases

    eggNOGi NOG292343.
    GeneTreei ENSGT00730000113838.
    OMAi HRIRIYV.
    OrthoDBi EOG7P2Z1W.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31541-MONOMER.

    Miscellaneous databases

    NextBioi 973783.

    Gene expression databases

    Genevestigatori P47029.

    Family and domain databases

    InterProi IPR011021. Arrestin-like_N.
    IPR011022. Arrestin_C-like.
    IPR014756. Ig_E-set.
    [Graphical view ]
    Pfami PF02752. Arrestin_C. 1 hit.
    PF00339. Arrestin_N. 1 hit.
    [Graphical view ]
    SMARTi SM01017. Arrestin_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF81296. SSF81296. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of a 33.1 kb fragment from the left arm of Saccharomyces cerevisiae chromosome X, including putative proteins with leucine zippers, a fungal Zn(II)2-Cys6 binuclear cluster domain and a putative alpha 2-SCB-alpha 2 binding site."
      Miosga T., Schaaff-Gerstenschlaeger I., Chalwatzis N., Baur A., Boles E., Fournier C., Schmitt S., Velten C., Wilhelm N., Zimmermann F.K.
      Yeast 11:681-689(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Analysis of phosphorylation of YJL084c, a yeast protein."
      Shi X.Z., Ao S.Z.
      Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao 34:433-438(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION, INTERACTION WITH PCL6 AND PCL7.
    5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
      Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
      Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-213, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: YAL6B.
    8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-155; SER-162 AND SER-838, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    9. "Ubiquitination screen using protein microarrays for comprehensive identification of Rsp5 substrates in yeast."
      Gupta R., Kus B., Fladd C., Wasmuth J., Tonikian R., Sidhu S., Krogan N.J., Parkinson J., Rotin D.
      Mol. Syst. Biol. 3:116-116(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RSP5, UBIQUITINATION BY RSP5.
    10. "Arrestin-related ubiquitin-ligase adaptors regulate endocytosis and protein turnover at the cell surface."
      Lin C.H., MacGurn J.A., Chu T., Stefan C.J., Emr S.D.
      Cell 135:714-725(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RPS5, FUNCTION.
    11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-155; SER-826; SER-838 AND SER-900, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-155; SER-586; SER-838; SER-900; SER-1022 AND SER-1023, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiALY2_YEAST
    AccessioniPrimary (citable) accession number: P47029
    Secondary accession number(s): D6VW99, Q05742
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 103 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 49 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3