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P47027

- DPB11_YEAST

UniProt

P47027 - DPB11_YEAST

Protein

DNA replication regulator DPB11

Gene

DPB11

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Has a role in the initiation of DNA replication. Required at S-phase checkpoint. Required for the association of PSF1 with origins. Also required for the proper activation of RAD53 in response to DNA damage and replication blocks. Multicopy suppressor of DPB2 mutation. Overexpression restores the growth defect conferred by POL2 mutation.3 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. protein kinase activator activity Source: SGD

    GO - Biological processi

    1. DNA replication checkpoint Source: SGD
    2. DNA replication initiation Source: SGD
    3. double-strand break repair via break-induced replication Source: SGD
    4. lagging strand elongation Source: SGD
    5. leading strand elongation Source: SGD
    6. mating type switching Source: SGD
    7. mismatch repair Source: SGD
    8. mitotic G2 DNA damage checkpoint Source: SGD
    9. nucleotide-excision repair Source: SGD
    10. positive regulation of protein kinase activity Source: GOC
    11. positive regulation of protein phosphorylation Source: SGD
    12. recombinational repair Source: SGD

    Keywords - Biological processi

    Cell cycle, DNA replication

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31545-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA replication regulator DPB11
    Gene namesi
    Name:DPB11
    Ordered Locus Names:YJL090C
    ORF Names:J0918
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome X

    Organism-specific databases

    CYGDiYJL090c.
    SGDiS000003626. DPB11.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. DNA replication preinitiation complex Source: SGD
    2. replication fork Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 764764DNA replication regulator DPB11PRO_0000079989Add
    BLAST

    Proteomic databases

    MaxQBiP47027.
    PaxDbiP47027.

    Expressioni

    Gene expression databases

    GenevestigatoriP47027.

    Interactioni

    Subunit structurei

    Interacts with SLD2.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    LCD1Q043776EBI-25984,EBI-35652
    MEC1P381112EBI-25984,EBI-6668
    RAD9P147379EBI-25984,EBI-14788
    SLD2P342525EBI-25984,EBI-26824

    Protein-protein interaction databases

    BioGridi33667. 97 interactions.
    DIPiDIP-5037N.
    IntActiP47027. 9 interactions.
    MINTiMINT-570502.
    STRINGi4932.YJL090C.

    Structurei

    3D structure databases

    ProteinModelPortaliP47027.
    SMRiP47027. Positions 2-88, 326-489.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 9999BRCT 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini129 – 22092BRCT 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini322 – 41897BRCT 3PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 3 BRCT domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG318746.
    HOGENOMiHOG000112252.
    KOiK03507.
    OMAiIWNSIMD.
    OrthoDBiEOG7HB5JW.

    Family and domain databases

    Gene3Di3.40.50.10190. 4 hits.
    InterProiIPR001357. BRCT_dom.
    [Graphical view]
    PfamiPF00533. BRCT. 3 hits.
    [Graphical view]
    SMARTiSM00292. BRCT. 3 hits.
    [Graphical view]
    SUPFAMiSSF52113. SSF52113. 5 hits.
    PROSITEiPS50172. BRCT. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P47027-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKPFQGITFC PTAINNEILA KKISKKIIKL GGIFSKDLTR QVNVLVVGST    50
    TNTNKFKFAV KHRFDIIFID IQAIDDIYQL WLSGENILPD SNTATMTGST 100
    YEMLKILYRR FSFKYLHNFN IFIGRITDTN ITSIDSLVRS IKKLGCSSYN 150
    YQNFVIKDTS SHNDDDDQGQ NGQISIFVTD TLLGARVNAA IEQNIPIVHF 200
    KWILDCQKRS ALLPYDPYYL LPNIKDLPYD SIGSNSCDCW DKINTTFPTN 250
    IDAQSSLQRQ QSSSTLTPSL PKTSSLLNKF KPKGEKIWDK AMSLQQHSKT 300
    NFSVLGQSPL SINNKQEDLS DNSTLIFKNC AFIIHHIFPG NHRSILTKIV 350
    VQNGGKIETS YLSGIYDHSY YIIPSNKALD SFNDLPEIID DNDGIVTEFF 400
    IERCLYYQKL LHPIDLWSKP FLSTIEFQVS SSSKLLHHEF SSSPFLNVTI 450
    TGFSGVELLH LTKVLNLLKP MGINYVEYLN KSTDILLINL AALPSIPKTH 500
    PLWSNEFSDL FTQFCINNNN DDPGDNNRKD FQNNSILRNS MKRKIEYIKK 550
    FHSIPVVTPA FIFKLLSAAS GENNEIFLNN IKWCIICPRG HKDDFKCKIK 600
    KPYYTSISSE KKYQNNDPKI DKTILLKRNN SSLSEHSMKD TKNELLQKIR 650
    ETDSGRKKRS VSSSIMDVSS ERQMPDTKRI KLESLPKNFV PKQIKRTTSW 700
    GTIMSENVPT EQPTAISNPE EIPRTEEVSH TQVTYGSIQD KKRTASLEKP 750
    MRRQTRNQTK ELDS 764
    Length:764
    Mass (Da):87,241
    Last modified:February 1, 1996 - v1
    Checksum:i79B3A9BC7815E440
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti72 – 721Q → K in BAA07725. (PubMed:8524850)Curated
    Sequence conflicti295 – 2962QQ → HR in BAA07725. (PubMed:8524850)Curated
    Sequence conflicti515 – 5151C → Y in BAA07725. (PubMed:8524850)Curated
    Sequence conflicti603 – 6031Y → C in BAA07725. (PubMed:8524850)Curated
    Sequence conflicti656 – 6561R → K in BAA07725. (PubMed:8524850)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D42168 Genomic DNA. Translation: BAA07725.2.
    X83502 Genomic DNA. Translation: CAA58477.1.
    Z49365 Genomic DNA. Translation: CAA89383.1.
    BK006943 Genomic DNA. Translation: DAA08710.1.
    PIRiS56017.
    RefSeqiNP_012445.1. NM_001181523.1.

    Genome annotation databases

    EnsemblFungiiYJL090C; YJL090C; YJL090C.
    GeneIDi853355.
    KEGGisce:YJL090C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D42168 Genomic DNA. Translation: BAA07725.2 .
    X83502 Genomic DNA. Translation: CAA58477.1 .
    Z49365 Genomic DNA. Translation: CAA89383.1 .
    BK006943 Genomic DNA. Translation: DAA08710.1 .
    PIRi S56017.
    RefSeqi NP_012445.1. NM_001181523.1.

    3D structure databases

    ProteinModelPortali P47027.
    SMRi P47027. Positions 2-88, 326-489.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33667. 97 interactions.
    DIPi DIP-5037N.
    IntActi P47027. 9 interactions.
    MINTi MINT-570502.
    STRINGi 4932.YJL090C.

    Proteomic databases

    MaxQBi P47027.
    PaxDbi P47027.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YJL090C ; YJL090C ; YJL090C .
    GeneIDi 853355.
    KEGGi sce:YJL090C.

    Organism-specific databases

    CYGDi YJL090c.
    SGDi S000003626. DPB11.

    Phylogenomic databases

    eggNOGi NOG318746.
    HOGENOMi HOG000112252.
    KOi K03507.
    OMAi IWNSIMD.
    OrthoDBi EOG7HB5JW.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31545-MONOMER.

    Miscellaneous databases

    NextBioi 973768.

    Gene expression databases

    Genevestigatori P47027.

    Family and domain databases

    Gene3Di 3.40.50.10190. 4 hits.
    InterProi IPR001357. BRCT_dom.
    [Graphical view ]
    Pfami PF00533. BRCT. 3 hits.
    [Graphical view ]
    SMARTi SM00292. BRCT. 3 hits.
    [Graphical view ]
    SUPFAMi SSF52113. SSF52113. 5 hits.
    PROSITEi PS50172. BRCT. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Dpb11, which interacts with DNA polymerase II(epsilon) in Saccharomyces cerevisiae, has a dual role in S-phase progression and at a cell cycle checkpoint."
      Araki H., Leem S.-H., Phongdara A., Sugino A.
      Proc. Natl. Acad. Sci. U.S.A. 92:11791-11795(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
      Strain: SK1.
    2. "Sequence analysis of a 33.1 kb fragment from the left arm of Saccharomyces cerevisiae chromosome X, including putative proteins with leucine zippers, a fungal Zn(II)2-Cys6 binuclear cluster domain and a putative alpha 2-SCB-alpha 2 binding site."
      Miosga T., Schaaff-Gerstenschlaeger I., Chalwatzis N., Baur A., Boles E., Fournier C., Schmitt S., Velten C., Wilhelm N., Zimmermann F.K.
      Yeast 11:681-689(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "DRC1, DNA replication and checkpoint protein 1, functions with DPB11 to control DNA replication and the S-phase checkpoint in Saccharomyces cerevisiae."
      Wang H., Elledge S.J.
      Proc. Natl. Acad. Sci. U.S.A. 96:3824-3829(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH SLD2.
    6. "GINS, a novel multiprotein complex required for chromosomal DNA replication in budding yeast."
      Takayama Y., Kamimura Y., Okawa M., Muramatsu S., Sugino A., Araki H.
      Genes Dev. 17:1153-1165(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    7. "Sld2, which interacts with Dpb11 in Saccharomyces cerevisiae, is required for chromosomal DNA replication."
      Kamimura Y., Masumoto H., Sugino A., Araki H.
      Mol. Cell. Biol. 18:6102-6109(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SLD2.
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiDPB11_YEAST
    AccessioniPrimary (citable) accession number: P47027
    Secondary accession number(s): D6VW94, Q05712
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 110 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 540 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3