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P47027 (DPB11_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA replication regulator DPB11
Gene names
Name:DPB11
Ordered Locus Names:YJL090C
ORF Names:J0918
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length764 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Has a role in the initiation of DNA replication. Required at S-phase checkpoint. Required for the association of PSF1 with origins. Also required for the proper activation of RAD53 in response to DNA damage and replication blocks. Multicopy suppressor of DPB2 mutation. Overexpression restores the growth defect conferred by POL2 mutation. Ref.1 Ref.5 Ref.6

Subunit structure

Interacts with SLD2. Ref.5 Ref.7

Subcellular location

Nucleus Ref.6.

Miscellaneous

Present with 540 molecules/cell in log phase SD medium.

Sequence similarities

Contains 3 BRCT domains.

Ontologies

Keywords
   Biological processCell cycle
DNA replication
   Cellular componentNucleus
   DomainRepeat
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA replication checkpoint

Inferred from mutant phenotype Ref.5. Source: SGD

DNA replication initiation

Traceable author statement Ref.6. Source: SGD

double-strand break repair via break-induced replication

Inferred from mutant phenotype PubMed 20516198. Source: SGD

lagging strand elongation

Traceable author statement PubMed 9745046. Source: SGD

leading strand elongation

Traceable author statement PubMed 9745046. Source: SGD

mating type switching

Inferred from mutant phenotype PubMed 21130053. Source: SGD

mismatch repair

Non-traceable author statement PubMed 10072354. Source: SGD

mitotic G2 DNA damage checkpoint

Inferred from genetic interaction PubMed 18541674. Source: SGD

nucleotide-excision repair

Traceable author statement PubMed 9745046. Source: SGD

positive regulation of protein kinase activity

Inferred from direct assay PubMed 18922789. Source: GOC

positive regulation of protein phosphorylation

Inferred from direct assay PubMed 18842580PubMed 18922789. Source: SGD

recombinational repair

Inferred from direct assay PubMed 16840526. Source: SGD

   Cellular_componentDNA replication preinitiation complex

Inferred from mutant phenotype PubMed 17167415. Source: SGD

replication fork

Inferred from direct assay PubMed 10733584. Source: SGD

   Molecular_functionprotein kinase activator activity

Inferred from direct assay PubMed 18922789. Source: SGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 764764DNA replication regulator DPB11
PRO_0000079989

Regions

Domain1 – 9999BRCT 1
Domain129 – 22092BRCT 2
Domain322 – 41897BRCT 3

Experimental info

Sequence conflict721Q → K in BAA07725. Ref.1
Sequence conflict295 – 2962QQ → HR in BAA07725. Ref.1
Sequence conflict5151C → Y in BAA07725. Ref.1
Sequence conflict6031Y → C in BAA07725. Ref.1
Sequence conflict6561R → K in BAA07725. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P47027 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: 79B3A9BC7815E440

FASTA76487,241
        10         20         30         40         50         60 
MKPFQGITFC PTAINNEILA KKISKKIIKL GGIFSKDLTR QVNVLVVGST TNTNKFKFAV 

        70         80         90        100        110        120 
KHRFDIIFID IQAIDDIYQL WLSGENILPD SNTATMTGST YEMLKILYRR FSFKYLHNFN 

       130        140        150        160        170        180 
IFIGRITDTN ITSIDSLVRS IKKLGCSSYN YQNFVIKDTS SHNDDDDQGQ NGQISIFVTD 

       190        200        210        220        230        240 
TLLGARVNAA IEQNIPIVHF KWILDCQKRS ALLPYDPYYL LPNIKDLPYD SIGSNSCDCW 

       250        260        270        280        290        300 
DKINTTFPTN IDAQSSLQRQ QSSSTLTPSL PKTSSLLNKF KPKGEKIWDK AMSLQQHSKT 

       310        320        330        340        350        360 
NFSVLGQSPL SINNKQEDLS DNSTLIFKNC AFIIHHIFPG NHRSILTKIV VQNGGKIETS 

       370        380        390        400        410        420 
YLSGIYDHSY YIIPSNKALD SFNDLPEIID DNDGIVTEFF IERCLYYQKL LHPIDLWSKP 

       430        440        450        460        470        480 
FLSTIEFQVS SSSKLLHHEF SSSPFLNVTI TGFSGVELLH LTKVLNLLKP MGINYVEYLN 

       490        500        510        520        530        540 
KSTDILLINL AALPSIPKTH PLWSNEFSDL FTQFCINNNN DDPGDNNRKD FQNNSILRNS 

       550        560        570        580        590        600 
MKRKIEYIKK FHSIPVVTPA FIFKLLSAAS GENNEIFLNN IKWCIICPRG HKDDFKCKIK 

       610        620        630        640        650        660 
KPYYTSISSE KKYQNNDPKI DKTILLKRNN SSLSEHSMKD TKNELLQKIR ETDSGRKKRS 

       670        680        690        700        710        720 
VSSSIMDVSS ERQMPDTKRI KLESLPKNFV PKQIKRTTSW GTIMSENVPT EQPTAISNPE 

       730        740        750        760 
EIPRTEEVSH TQVTYGSIQD KKRTASLEKP MRRQTRNQTK ELDS 

« Hide

References

« Hide 'large scale' references
[1]"Dpb11, which interacts with DNA polymerase II(epsilon) in Saccharomyces cerevisiae, has a dual role in S-phase progression and at a cell cycle checkpoint."
Araki H., Leem S.-H., Phongdara A., Sugino A.
Proc. Natl. Acad. Sci. U.S.A. 92:11791-11795(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
Strain: SK1.
[2]"Sequence analysis of a 33.1 kb fragment from the left arm of Saccharomyces cerevisiae chromosome X, including putative proteins with leucine zippers, a fungal Zn(II)2-Cys6 binuclear cluster domain and a putative alpha 2-SCB-alpha 2 binding site."
Miosga T., Schaaff-Gerstenschlaeger I., Chalwatzis N., Baur A., Boles E., Fournier C., Schmitt S., Velten C., Wilhelm N., Zimmermann F.K.
Yeast 11:681-689(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"DRC1, DNA replication and checkpoint protein 1, functions with DPB11 to control DNA replication and the S-phase checkpoint in Saccharomyces cerevisiae."
Wang H., Elledge S.J.
Proc. Natl. Acad. Sci. U.S.A. 96:3824-3829(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH SLD2.
[6]"GINS, a novel multiprotein complex required for chromosomal DNA replication in budding yeast."
Takayama Y., Kamimura Y., Okawa M., Muramatsu S., Sugino A., Araki H.
Genes Dev. 17:1153-1165(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[7]"Sld2, which interacts with Dpb11 in Saccharomyces cerevisiae, is required for chromosomal DNA replication."
Kamimura Y., Masumoto H., Sugino A., Araki H.
Mol. Cell. Biol. 18:6102-6109(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SLD2.
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D42168 Genomic DNA. Translation: BAA07725.2.
X83502 Genomic DNA. Translation: CAA58477.1.
Z49365 Genomic DNA. Translation: CAA89383.1.
BK006943 Genomic DNA. Translation: DAA08710.1.
PIRS56017.
RefSeqNP_012445.1. NM_001181523.1.

3D structure databases

ProteinModelPortalP47027.
SMRP47027. Positions 2-223, 326-580.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33667. 95 interactions.
DIPDIP-5037N.
IntActP47027. 9 interactions.
MINTMINT-570502.
STRING4932.YJL090C.

Proteomic databases

PaxDbP47027.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJL090C; YJL090C; YJL090C.
GeneID853355.
KEGGsce:YJL090C.

Organism-specific databases

CYGDYJL090c.
SGDS000003626. DPB11.

Phylogenomic databases

eggNOGNOG318746.
HOGENOMHOG000112252.
KOK03507.
OMAGIELLHI.
OrthoDBEOG7HB5JW.

Enzyme and pathway databases

BioCycYEAST:G3O-31545-MONOMER.

Gene expression databases

GenevestigatorP47027.

Family and domain databases

Gene3D3.40.50.10190. 4 hits.
InterProIPR001357. BRCT_dom.
[Graphical view]
PfamPF00533. BRCT. 3 hits.
[Graphical view]
SMARTSM00292. BRCT. 3 hits.
[Graphical view]
SUPFAMSSF52113. SSF52113. 5 hits.
PROSITEPS50172. BRCT. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio973768.

Entry information

Entry nameDPB11_YEAST
AccessionPrimary (citable) accession number: P47027
Secondary accession number(s): D6VW94, Q05712
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: April 16, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families