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P47027

- DPB11_YEAST

UniProt

P47027 - DPB11_YEAST

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Protein

DNA replication regulator DPB11

Gene

DPB11

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Has a role in the initiation of DNA replication. Required at S-phase checkpoint. Required for the association of PSF1 with origins. Also required for the proper activation of RAD53 in response to DNA damage and replication blocks. Multicopy suppressor of DPB2 mutation. Overexpression restores the growth defect conferred by POL2 mutation.3 Publications

GO - Molecular functioni

  1. protein kinase activator activity Source: SGD

GO - Biological processi

  1. DNA replication checkpoint Source: SGD
  2. DNA replication initiation Source: SGD
  3. double-strand break repair via break-induced replication Source: SGD
  4. lagging strand elongation Source: SGD
  5. leading strand elongation Source: SGD
  6. mating type switching Source: SGD
  7. mismatch repair Source: SGD
  8. mitotic G2 DNA damage checkpoint Source: SGD
  9. nucleotide-excision repair Source: SGD
  10. positive regulation of protein kinase activity Source: GOC
  11. positive regulation of protein phosphorylation Source: SGD
  12. recombinational repair Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, DNA replication

Enzyme and pathway databases

BioCyciYEAST:G3O-31545-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication regulator DPB11
Gene namesi
Name:DPB11
Ordered Locus Names:YJL090C
ORF Names:J0918
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

CYGDiYJL090c.
SGDiS000003626. DPB11.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. DNA replication preinitiation complex Source: SGD
  2. replication fork Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 764764DNA replication regulator DPB11PRO_0000079989Add
BLAST

Proteomic databases

MaxQBiP47027.
PaxDbiP47027.

Expressioni

Gene expression databases

GenevestigatoriP47027.

Interactioni

Subunit structurei

Interacts with SLD2.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
LCD1Q043776EBI-25984,EBI-35652
MEC1P381112EBI-25984,EBI-6668
RAD9P147379EBI-25984,EBI-14788
SLD2P342525EBI-25984,EBI-26824

Protein-protein interaction databases

BioGridi33667. 98 interactions.
DIPiDIP-5037N.
IntActiP47027. 9 interactions.
MINTiMINT-570502.
STRINGi4932.YJL090C.

Structurei

3D structure databases

ProteinModelPortaliP47027.
SMRiP47027. Positions 2-88, 326-489.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 9999BRCT 1PROSITE-ProRule annotationAdd
BLAST
Domaini129 – 22092BRCT 2PROSITE-ProRule annotationAdd
BLAST
Domaini322 – 41897BRCT 3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 3 BRCT domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG318746.
HOGENOMiHOG000112252.
InParanoidiP47027.
KOiK03507.
OMAiIWNSIMD.
OrthoDBiEOG7HB5JW.

Family and domain databases

Gene3Di3.40.50.10190. 4 hits.
InterProiIPR001357. BRCT_dom.
[Graphical view]
PfamiPF00533. BRCT. 3 hits.
[Graphical view]
SMARTiSM00292. BRCT. 3 hits.
[Graphical view]
SUPFAMiSSF52113. SSF52113. 5 hits.
PROSITEiPS50172. BRCT. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47027-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKPFQGITFC PTAINNEILA KKISKKIIKL GGIFSKDLTR QVNVLVVGST
60 70 80 90 100
TNTNKFKFAV KHRFDIIFID IQAIDDIYQL WLSGENILPD SNTATMTGST
110 120 130 140 150
YEMLKILYRR FSFKYLHNFN IFIGRITDTN ITSIDSLVRS IKKLGCSSYN
160 170 180 190 200
YQNFVIKDTS SHNDDDDQGQ NGQISIFVTD TLLGARVNAA IEQNIPIVHF
210 220 230 240 250
KWILDCQKRS ALLPYDPYYL LPNIKDLPYD SIGSNSCDCW DKINTTFPTN
260 270 280 290 300
IDAQSSLQRQ QSSSTLTPSL PKTSSLLNKF KPKGEKIWDK AMSLQQHSKT
310 320 330 340 350
NFSVLGQSPL SINNKQEDLS DNSTLIFKNC AFIIHHIFPG NHRSILTKIV
360 370 380 390 400
VQNGGKIETS YLSGIYDHSY YIIPSNKALD SFNDLPEIID DNDGIVTEFF
410 420 430 440 450
IERCLYYQKL LHPIDLWSKP FLSTIEFQVS SSSKLLHHEF SSSPFLNVTI
460 470 480 490 500
TGFSGVELLH LTKVLNLLKP MGINYVEYLN KSTDILLINL AALPSIPKTH
510 520 530 540 550
PLWSNEFSDL FTQFCINNNN DDPGDNNRKD FQNNSILRNS MKRKIEYIKK
560 570 580 590 600
FHSIPVVTPA FIFKLLSAAS GENNEIFLNN IKWCIICPRG HKDDFKCKIK
610 620 630 640 650
KPYYTSISSE KKYQNNDPKI DKTILLKRNN SSLSEHSMKD TKNELLQKIR
660 670 680 690 700
ETDSGRKKRS VSSSIMDVSS ERQMPDTKRI KLESLPKNFV PKQIKRTTSW
710 720 730 740 750
GTIMSENVPT EQPTAISNPE EIPRTEEVSH TQVTYGSIQD KKRTASLEKP
760
MRRQTRNQTK ELDS
Length:764
Mass (Da):87,241
Last modified:February 1, 1996 - v1
Checksum:i79B3A9BC7815E440
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti72 – 721Q → K in BAA07725. (PubMed:8524850)Curated
Sequence conflicti295 – 2962QQ → HR in BAA07725. (PubMed:8524850)Curated
Sequence conflicti515 – 5151C → Y in BAA07725. (PubMed:8524850)Curated
Sequence conflicti603 – 6031Y → C in BAA07725. (PubMed:8524850)Curated
Sequence conflicti656 – 6561R → K in BAA07725. (PubMed:8524850)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D42168 Genomic DNA. Translation: BAA07725.2.
X83502 Genomic DNA. Translation: CAA58477.1.
Z49365 Genomic DNA. Translation: CAA89383.1.
BK006943 Genomic DNA. Translation: DAA08710.1.
PIRiS56017.
RefSeqiNP_012445.1. NM_001181523.1.

Genome annotation databases

EnsemblFungiiYJL090C; YJL090C; YJL090C.
GeneIDi853355.
KEGGisce:YJL090C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D42168 Genomic DNA. Translation: BAA07725.2 .
X83502 Genomic DNA. Translation: CAA58477.1 .
Z49365 Genomic DNA. Translation: CAA89383.1 .
BK006943 Genomic DNA. Translation: DAA08710.1 .
PIRi S56017.
RefSeqi NP_012445.1. NM_001181523.1.

3D structure databases

ProteinModelPortali P47027.
SMRi P47027. Positions 2-88, 326-489.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33667. 98 interactions.
DIPi DIP-5037N.
IntActi P47027. 9 interactions.
MINTi MINT-570502.
STRINGi 4932.YJL090C.

Proteomic databases

MaxQBi P47027.
PaxDbi P47027.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YJL090C ; YJL090C ; YJL090C .
GeneIDi 853355.
KEGGi sce:YJL090C.

Organism-specific databases

CYGDi YJL090c.
SGDi S000003626. DPB11.

Phylogenomic databases

eggNOGi NOG318746.
HOGENOMi HOG000112252.
InParanoidi P47027.
KOi K03507.
OMAi IWNSIMD.
OrthoDBi EOG7HB5JW.

Enzyme and pathway databases

BioCyci YEAST:G3O-31545-MONOMER.

Miscellaneous databases

NextBioi 973768.

Gene expression databases

Genevestigatori P47027.

Family and domain databases

Gene3Di 3.40.50.10190. 4 hits.
InterProi IPR001357. BRCT_dom.
[Graphical view ]
Pfami PF00533. BRCT. 3 hits.
[Graphical view ]
SMARTi SM00292. BRCT. 3 hits.
[Graphical view ]
SUPFAMi SSF52113. SSF52113. 5 hits.
PROSITEi PS50172. BRCT. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Dpb11, which interacts with DNA polymerase II(epsilon) in Saccharomyces cerevisiae, has a dual role in S-phase progression and at a cell cycle checkpoint."
    Araki H., Leem S.-H., Phongdara A., Sugino A.
    Proc. Natl. Acad. Sci. U.S.A. 92:11791-11795(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    Strain: SK1.
  2. "Sequence analysis of a 33.1 kb fragment from the left arm of Saccharomyces cerevisiae chromosome X, including putative proteins with leucine zippers, a fungal Zn(II)2-Cys6 binuclear cluster domain and a putative alpha 2-SCB-alpha 2 binding site."
    Miosga T., Schaaff-Gerstenschlaeger I., Chalwatzis N., Baur A., Boles E., Fournier C., Schmitt S., Velten C., Wilhelm N., Zimmermann F.K.
    Yeast 11:681-689(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "DRC1, DNA replication and checkpoint protein 1, functions with DPB11 to control DNA replication and the S-phase checkpoint in Saccharomyces cerevisiae."
    Wang H., Elledge S.J.
    Proc. Natl. Acad. Sci. U.S.A. 96:3824-3829(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SLD2.
  6. "GINS, a novel multiprotein complex required for chromosomal DNA replication in budding yeast."
    Takayama Y., Kamimura Y., Okawa M., Muramatsu S., Sugino A., Araki H.
    Genes Dev. 17:1153-1165(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  7. "Sld2, which interacts with Dpb11 in Saccharomyces cerevisiae, is required for chromosomal DNA replication."
    Kamimura Y., Masumoto H., Sugino A., Araki H.
    Mol. Cell. Biol. 18:6102-6109(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SLD2.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiDPB11_YEAST
AccessioniPrimary (citable) accession number: P47027
Secondary accession number(s): D6VW94, Q05712
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: October 29, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 540 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3