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Protein

Mitochondrial division protein 1

Gene

MDV1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in mitochondrial fission. Has a partially redundant function to CAF4 in acting as an adapter protein, binding to FIS1 on the mitochondrial outer membrane and recruiting the dynamin-like GTPase DNM1 to form mitochondrial fission complexes. Formation of these complexes is required to promote constriction and fission of the mitochondrial compartment at a late step in mitochondrial division.8 Publications

Miscellaneous

Present with 3730 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • ubiquitin binding Source: SGD

GO - Biological processi

  • mitochondrial fission Source: SGD
  • mitochondrial genome maintenance Source: SGD
  • peroxisome fission Source: SGD
  • positive regulation of mitochondrial fission Source: UniProtKB

Enzyme and pathway databases

BioCyciYEAST:G3O-31566-MONOMER
ReactomeiR-SCE-1266695 Interleukin-7 signaling
R-SCE-3247509 Chromatin modifying enzymes

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial division protein 1
Alternative name(s):
Mitochondria fission 2 protein
Gene namesi
Name:MDV1
Synonyms:FIS2, GAG3, NET2
Ordered Locus Names:YJL112W
ORF Names:J0802
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJL112W
SGDiS000003648 MDV1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi148L → P: Abolishes interaction with FIS1. 1 Publication1
Mutagenesisi250E → G: Suppresses the mitochondrial fission defect of a FIS1-3 mutant by affecting interaction with FIS1. 1 Publication1
Mutagenesisi400F → A: No interaction with FIS1; slight decrease in interaction with DNM1. 1 Publication1
Mutagenesisi440H → E or N: Abolishes interaction with DNM1. 1 Publication1
Mutagenesisi461R → A: Slight decrease in interaction with FIS1; 20-fold decrease in interaction with DNM1; even distribution along mitochondria not punctate. 1 Publication1
Mutagenesisi544N → R: Results in mitochondrial division defect, but has no effect on the interaction with DNM1. 1 Publication1
Mutagenesisi605H → E: Abolishes interaction with DNM1. 1 Publication1
Mutagenesisi664D → A: Slight decrease in interaction with DNM1. 1 Publication1
Mutagenesisi689S → A: Slight decrease in interaction with DNM1. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000514771 – 714Mitochondrial division protein 1Add BLAST714

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei376PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP47025
PaxDbiP47025
PRIDEiP47025

PTM databases

iPTMnetiP47025

Interactioni

Subunit structurei

Interacts with CAF4, DNM1 and FIS1, components of the mitochondrial fission machinery. Interacts via its N-terminal, coiled-coil extension (NTE) with FIS1, and via its WD repeats with DNM1.8 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
FIS1P405155EBI-26032,EBI-25059

GO - Molecular functioni

  • ubiquitin binding Source: SGD

Protein-protein interaction databases

BioGridi33644, 114 interactors
DIPiDIP-1907N
IntActiP47025, 12 interactors
MINTiP47025
STRINGi4932.YJL112W

Structurei

Secondary structure

1714
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi148 – 161Combined sources14
Helixi231 – 289Combined sources59

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2PQNX-ray2.15B122-171[»]
2XU6X-ray2.70A/B231-300[»]
3UUXX-ray3.90B/D94-314[»]
5JSTX-ray2.20A/B230-300[»]
ProteinModelPortaliP47025
SMRiP47025
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP47025

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati396 – 436WD 1Add BLAST41
Repeati439 – 478WD 2Add BLAST40
Repeati500 – 539WD 3Add BLAST40
Repeati561 – 603WD 4Add BLAST43
Repeati604 – 642WD 5Add BLAST39
Repeati644 – 681WD 6Add BLAST38
Repeati685 – 714WD 7Add BLAST30

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili240 – 298Sequence analysisAdd BLAST59

Sequence similaritiesi

Belongs to the WD repeat MDV1/CAF4 family.Curated

Keywords - Domaini

Coiled coil, Repeat, WD repeat

Phylogenomic databases

GeneTreeiENSGT00920000149157
HOGENOMiHOG000048466
InParanoidiP47025
KOiK17970
OMAiNAGRCIG
OrthoDBiEOG092C172M

Family and domain databases

Gene3Di2.130.10.10, 2 hits
InterProiView protein in InterPro
IPR020472 G-protein_beta_WD-40_rep
IPR021061 Mt_division_protein_1
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF11542 Mdv1, 1 hit
PF00400 WD40, 4 hits
PRINTSiPR00320 GPROTEINBRPT
SMARTiView protein in SMART
SM00320 WD40, 6 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 4 hits
PS50082 WD_REPEATS_2, 6 hits
PS50294 WD_REPEATS_REGION, 1 hit

Sequencei

Sequence statusi: Complete.

P47025-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVNDQITHI GKTLSTTASA FLNYQKSNSN TQDVLTNNGP YKNLLSNTVN
60 70 80 90 100
NASSTSYFYK RTEHGRFVKN ASNTFEDIYS KTRRGDVFRN KFTDNKTCFR
110 120 130 140 150
MLTYISDDLL NEIPTKEGLK SDADGKLLTE GGENENLRKN ASKKETSLFQ
160 170 180 190 200
GFKSYLPIAE LAIENTERLN YDTNGTSGTV GAKDVMSKTN ERDEIHTELP
210 220 230 240 250
NFQDSFLIPP GVETKKISSS YSPSALKSFS QTLVNSLEFL NIQKNSTLSE
260 270 280 290 300
IRDIEVEVEN LRQKKEKLLG KIANIEQNQL LLEDNLKQID DRLDFLEEYG
310 320 330 340 350
LEVIEANSDE NAEDDGMSER KALKNDAIRN EGVTTESISS EASNLPPRRR
360 370 380 390 400
QQLRDDNSLN RLGAFYSKSK KRHRKSFPTF QQLYEPGTKI GSIMSTHDDF
410 420 430 440 450
LTCLDFDAPF GTLCTAGYLD HTVKIWDLSK QNKIGELAGH LATINCMQIN
460 470 480 490 500
RDYGTLVTGG RDAALKLWNL NLAQQLYQET QNLTSPTNHI DSPCVHTFEA
510 520 530 540 550
HTDEVTALSL DPSFLVSGSQ DRTIRQWDLR SGKCLQTIDL SFANVLTTST
560 570 580 590 600
NVDLSKSTLL TQRNERPSIG ALQSFDAALA TGTKDGVVRL WDLRSGKVIR
610 620 630 640 650
TLKGHTDAIT SLKFDSACLV TGSYDRTVRI WDLRTGLLNK FHAYSAPVLS
660 670 680 690 700
LDLFQENAAV VVADEPSVQI YDSEKDESWS CVEQGNETSV STVKYKENYM
710
VEGRENGDVN IWAV
Length:714
Mass (Da):80,032
Last modified:February 1, 1996 - v1
Checksum:iCA8A23DBB06193A6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49387 Genomic DNA Translation: CAA89407.1
BK006943 Genomic DNA Translation: DAA08688.1
PIRiS56893
RefSeqiNP_012423.1, NM_001181545.2

Genome annotation databases

EnsemblFungiiYJL112W; YJL112W; YJL112W
GeneIDi853332
KEGGisce:YJL112W

Similar proteinsi

Entry informationi

Entry nameiMDV1_YEAST
AccessioniPrimary (citable) accession number: P47025
Secondary accession number(s): D6VW72
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: June 20, 2018
This is version 159 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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