Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Mitochondrial division protein 1

Gene

MDV1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in mitochondrial fission. Has a partially redundant function to CAF4 in acting as an adapter protein, binding to FIS1 on the mitochondrial outer membrane and recruiting the dynamin-like GTPase DNM1 to form mitochondrial fission complexes. Formation of these complexes is required to promote constriction and fission of the mitochondrial compartment at a late step in mitochondrial division.8 Publications

GO - Molecular functioni

  • ubiquitin binding Source: SGD

GO - Biological processi

  • mitochondrial fission Source: SGD
  • mitochondrial genome maintenance Source: SGD
  • peroxisome fission Source: SGD
  • positive regulation of mitochondrial fission Source: UniProtKB
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-31566-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial division protein 1
Alternative name(s):
Mitochondria fission 2 protein
Gene namesi
Name:MDV1
Synonyms:FIS2, GAG3, NET2
Ordered Locus Names:YJL112W
ORF Names:J0802
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJL112W.
SGDiS000003648. MDV1.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial outer membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi148 – 1481L → P: Abolishes interaction with FIS1. 1 Publication
Mutagenesisi250 – 2501E → G: Suppresses the mitochondrial fission defect of a FIS1-3 mutant by affecting interaction with FIS1. 1 Publication
Mutagenesisi400 – 4001F → A: No interaction with FIS1; slight decrease in interaction with DNM1. 1 Publication
Mutagenesisi440 – 4401H → E or N: Abolishes interaction with DNM1. 1 Publication
Mutagenesisi461 – 4611R → A: Slight decrease in interaction with FIS1; 20-fold decrease in interaction with DNM1; even distribution along mitochondria not punctate. 1 Publication
Mutagenesisi544 – 5441N → R: Results in mitrochondrial division defect, but has no effect on the interaction with DNM1. 1 Publication
Mutagenesisi605 – 6051H → E: Abolishes interaction with DNM1. 1 Publication
Mutagenesisi664 – 6641D → A: Slight decrease in interaction with DNM1. 1 Publication
Mutagenesisi689 – 6891S → A: Slight decrease in interaction with DNM1. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 714714Mitochondrial division protein 1PRO_0000051477Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei376 – 3761PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP47025.

PTM databases

iPTMnetiP47025.

Interactioni

Subunit structurei

Interacts with CAF4, DNM1 and FIS1, components of the mitochondrial fission machinery. Interacts via its N-terminal, coiled-coil extension (NTE) with FIS1, and via its WD repeats with DNM1.8 Publications

GO - Molecular functioni

  • ubiquitin binding Source: SGD

Protein-protein interaction databases

BioGridi33644. 59 interactions.
DIPiDIP-1907N.
IntActiP47025. 12 interactions.
MINTiMINT-389558.

Structurei

Secondary structure

1
714
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi148 – 16114Combined sources
Helixi231 – 28959Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2PQNX-ray2.15B122-171[»]
2XU6X-ray2.70A/B231-300[»]
3UUXX-ray3.90B/D94-314[»]
ProteinModelPortaliP47025.
SMRiP47025. Positions 94-301, 342-714.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP47025.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati396 – 43641WD 1Add
BLAST
Repeati439 – 47840WD 2Add
BLAST
Repeati500 – 53940WD 3Add
BLAST
Repeati561 – 60343WD 4Add
BLAST
Repeati604 – 64239WD 5Add
BLAST
Repeati644 – 68138WD 6Add
BLAST
Repeati685 – 71430WD 7Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili240 – 29859Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the WD repeat MDV1/CAF4 family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat, WD repeat

Phylogenomic databases

GeneTreeiENSGT00820000127095.
HOGENOMiHOG000048466.
InParanoidiP47025.
KOiK17970.
OMAiIRERIDM.
OrthoDBiEOG092C172M.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR021061. Mt_division_protein_1.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF11542. Mdv1. 1 hit.
PF00400. WD40. 4 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 4 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47025-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVNDQITHI GKTLSTTASA FLNYQKSNSN TQDVLTNNGP YKNLLSNTVN
60 70 80 90 100
NASSTSYFYK RTEHGRFVKN ASNTFEDIYS KTRRGDVFRN KFTDNKTCFR
110 120 130 140 150
MLTYISDDLL NEIPTKEGLK SDADGKLLTE GGENENLRKN ASKKETSLFQ
160 170 180 190 200
GFKSYLPIAE LAIENTERLN YDTNGTSGTV GAKDVMSKTN ERDEIHTELP
210 220 230 240 250
NFQDSFLIPP GVETKKISSS YSPSALKSFS QTLVNSLEFL NIQKNSTLSE
260 270 280 290 300
IRDIEVEVEN LRQKKEKLLG KIANIEQNQL LLEDNLKQID DRLDFLEEYG
310 320 330 340 350
LEVIEANSDE NAEDDGMSER KALKNDAIRN EGVTTESISS EASNLPPRRR
360 370 380 390 400
QQLRDDNSLN RLGAFYSKSK KRHRKSFPTF QQLYEPGTKI GSIMSTHDDF
410 420 430 440 450
LTCLDFDAPF GTLCTAGYLD HTVKIWDLSK QNKIGELAGH LATINCMQIN
460 470 480 490 500
RDYGTLVTGG RDAALKLWNL NLAQQLYQET QNLTSPTNHI DSPCVHTFEA
510 520 530 540 550
HTDEVTALSL DPSFLVSGSQ DRTIRQWDLR SGKCLQTIDL SFANVLTTST
560 570 580 590 600
NVDLSKSTLL TQRNERPSIG ALQSFDAALA TGTKDGVVRL WDLRSGKVIR
610 620 630 640 650
TLKGHTDAIT SLKFDSACLV TGSYDRTVRI WDLRTGLLNK FHAYSAPVLS
660 670 680 690 700
LDLFQENAAV VVADEPSVQI YDSEKDESWS CVEQGNETSV STVKYKENYM
710
VEGRENGDVN IWAV
Length:714
Mass (Da):80,032
Last modified:February 1, 1996 - v1
Checksum:iCA8A23DBB06193A6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49387 Genomic DNA. Translation: CAA89407.1.
BK006943 Genomic DNA. Translation: DAA08688.1.
PIRiS56893.
RefSeqiNP_012423.1. NM_001181545.2.

Genome annotation databases

EnsemblFungiiYJL112W; YJL112W; YJL112W.
GeneIDi853332.
KEGGisce:YJL112W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49387 Genomic DNA. Translation: CAA89407.1.
BK006943 Genomic DNA. Translation: DAA08688.1.
PIRiS56893.
RefSeqiNP_012423.1. NM_001181545.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2PQNX-ray2.15B122-171[»]
2XU6X-ray2.70A/B231-300[»]
3UUXX-ray3.90B/D94-314[»]
ProteinModelPortaliP47025.
SMRiP47025. Positions 94-301, 342-714.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33644. 59 interactions.
DIPiDIP-1907N.
IntActiP47025. 12 interactions.
MINTiMINT-389558.

PTM databases

iPTMnetiP47025.

Proteomic databases

MaxQBiP47025.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJL112W; YJL112W; YJL112W.
GeneIDi853332.
KEGGisce:YJL112W.

Organism-specific databases

EuPathDBiFungiDB:YJL112W.
SGDiS000003648. MDV1.

Phylogenomic databases

GeneTreeiENSGT00820000127095.
HOGENOMiHOG000048466.
InParanoidiP47025.
KOiK17970.
OMAiIRERIDM.
OrthoDBiEOG092C172M.

Enzyme and pathway databases

BioCyciYEAST:G3O-31566-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP47025.
PROiP47025.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR021061. Mt_division_protein_1.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF11542. Mdv1. 1 hit.
PF00400. WD40. 4 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 4 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMDV1_YEAST
AccessioniPrimary (citable) accession number: P47025
Secondary accession number(s): D6VW72
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: September 7, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3730 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.