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Protein

Dihydrosphingosine 1-phosphate phosphatase LCB3

Gene

LCB3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Dihydrosphingosine 1-phosphate phosphatase required for efficient ceramide synthesis from exogenous sphingoid bases. Involved in endocytosis and calcium-mediated signaling.11 Publications

GO - Molecular functioni

  • sphingosine-1-phosphate phosphatase activity Source: SGD

GO - Biological processi

  • calcium-mediated signaling Source: SGD
  • sphingolipid biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid metabolism, Sphingolipid metabolism

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER3O-419.
YEAST:MONOMER3O-419.
ReactomeiR-SCE-1660661. Sphingolipid de novo biosynthesis.

Chemistry

SwissLipidsiSLP:000000932.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrosphingosine 1-phosphate phosphatase LCB3 (EC:3.1.3.-)
Alternative name(s):
Long-chain base protein 3
Sphingolipid resistance protein 2
Gene namesi
Name:LCB3
Synonyms:LBP1, YSR2
Ordered Locus Names:YJL134W
ORF Names:J0671
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJL134W.
SGDiS000003670. LCB3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 8686LumenalAdd
BLAST
Transmembranei87 – 10721Helical; Name=1Add
BLAST
Topological domaini108 – 1125Cytoplasmic
Transmembranei113 – 13321Helical; Name=2Add
BLAST
Topological domaini134 – 18249LumenalAdd
BLAST
Transmembranei183 – 20321Helical; Name=3Add
BLAST
Topological domaini204 – 2074Cytoplasmic
Transmembranei208 – 22821Helical; Name=4Add
BLAST
Topological domaini229 – 24517LumenalAdd
BLAST
Transmembranei246 – 26621Helical; Name=5Add
BLAST
Topological domaini267 – 27610Cytoplasmic
Transmembranei277 – 29721Helical; Name=6Add
BLAST
Topological domaini298 – 31518LumenalAdd
BLAST
Transmembranei316 – 33621Helical; Name=7Add
BLAST
Topological domaini337 – 38448CytoplasmicAdd
BLAST
Transmembranei385 – 40521Helical; Name=8Add
BLAST
Topological domaini406 – 4094Lumenal

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi128 – 1281K → A: Impairs dihydrosphingosine 1-phosphate phosphatase activity. 1 Publication
Mutagenesisi160 – 1601H → A: Impairs dihydrosphingosine 1-phosphate phosphatase activity. 1 Publication
Mutagenesisi210 – 2101H → A: Impairs dihydrosphingosine 1-phosphate phosphatase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 409409Dihydrosphingosine 1-phosphate phosphatase LCB3PRO_0000203037Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei16 – 161PhosphothreonineCombined sources
Modified residuei18 – 181PhosphoserineCombined sources

Post-translational modificationi

Glycosylated.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP47013.

PTM databases

iPTMnetiP47013.

Interactioni

Protein-protein interaction databases

BioGridi33622. 77 interactions.
DIPiDIP-5685N.
IntActiP47013. 4 interactions.
MINTiMINT-572017.

Structurei

3D structure databases

ProteinModelPortaliP47013.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000017322.
HOGENOMiHOG000066080.
InParanoidiP47013.
KOiK04716.
OMAiLMGSHTF.
OrthoDBiEOG092C3MDD.

Family and domain databases

Gene3Di1.20.144.10. 1 hit.
InterProiIPR000326. P_Acid_Pase_2/haloperoxidase.
[Graphical view]
PfamiPF01569. PAP2. 1 hit.
[Graphical view]
SMARTiSM00014. acidPPc. 1 hit.
[Graphical view]
SUPFAMiSSF48317. SSF48317. 1 hit.

Sequencei

Sequence statusi: Complete.

P47013-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDGLNTSNI RKRARTLSNP NDFQEPNYLL DPGNHPSDHF RTRMSKFRFN
60 70 80 90 100
IREKLLVFTN NQSFTLSRWQ KKYRSAFNDL YFTYTSLMGS HTFYVLCLPM
110 120 130 140 150
PVWFGYFETT KDMVYILGYS IYLSGFFKDY WCLPRPRAPP LHRITLSEYT
160 170 180 190 200
TKEYGAPSSH TANATGVSLL FLYNIWRMQE SSVMVQLLLS CVVLFYYMTL
210 220 230 240 250
VFGRIYCGMH GILDLVSGGL IGIVCFIVRM YFKYRFPGLR IEEHWWFPLF
260 270 280 290 300
SVGWGLLLLF KHVKPVDECP CFQDSVAFMG VVSGIECCDW LGKVFGVTLV
310 320 330 340 350
YNLEPNCGWR LTLARLLVGL PCVVIWKYVI SKPMIYTLLI KVFHLKDDRN
360 370 380 390 400
VAARKRLEAT HKEGASKYEC PLYIGEPKID ILGRFIIYAG VPFTVVMCSP

VLFSLLNIA
Length:409
Mass (Da):47,372
Last modified:February 1, 1996 - v1
Checksum:iE0CD7D0F169447C3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87371 Genomic DNA. Translation: CAA60821.1.
Z49410 Genomic DNA. Translation: CAA89430.1.
BK006943 Genomic DNA. Translation: DAA08666.1.
PIRiS55178.
RefSeqiNP_012401.1. NM_001181567.1.

Genome annotation databases

EnsemblFungiiYJL134W; YJL134W; YJL134W.
GeneIDi853307.
KEGGisce:YJL134W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87371 Genomic DNA. Translation: CAA60821.1.
Z49410 Genomic DNA. Translation: CAA89430.1.
BK006943 Genomic DNA. Translation: DAA08666.1.
PIRiS55178.
RefSeqiNP_012401.1. NM_001181567.1.

3D structure databases

ProteinModelPortaliP47013.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33622. 77 interactions.
DIPiDIP-5685N.
IntActiP47013. 4 interactions.
MINTiMINT-572017.

Chemistry

SwissLipidsiSLP:000000932.

PTM databases

iPTMnetiP47013.

Proteomic databases

MaxQBiP47013.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJL134W; YJL134W; YJL134W.
GeneIDi853307.
KEGGisce:YJL134W.

Organism-specific databases

EuPathDBiFungiDB:YJL134W.
SGDiS000003670. LCB3.

Phylogenomic databases

GeneTreeiENSGT00390000017322.
HOGENOMiHOG000066080.
InParanoidiP47013.
KOiK04716.
OMAiLMGSHTF.
OrthoDBiEOG092C3MDD.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER3O-419.
YEAST:MONOMER3O-419.
ReactomeiR-SCE-1660661. Sphingolipid de novo biosynthesis.

Miscellaneous databases

PROiP47013.

Family and domain databases

Gene3Di1.20.144.10. 1 hit.
InterProiIPR000326. P_Acid_Pase_2/haloperoxidase.
[Graphical view]
PfamiPF01569. PAP2. 1 hit.
[Graphical view]
SMARTiSM00014. acidPPc. 1 hit.
[Graphical view]
SUPFAMiSSF48317. SSF48317. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDS1P1_YEAST
AccessioniPrimary (citable) accession number: P47013
Secondary accession number(s): D6VW50
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: September 7, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.