Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Dihydrosphingosine 1-phosphate phosphatase LCB3

Gene

LCB3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Dihydrosphingosine 1-phosphate phosphatase required for efficient ceramide synthesis from exogenous sphingoid bases. Involved in endocytosis and calcium-mediated signaling.11 Publications

GO - Molecular functioni

  • sphingosine-1-phosphate phosphatase activity Source: SGD

GO - Biological processi

  • calcium-mediated signaling Source: SGD
  • sphingolipid biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid metabolism, Sphingolipid metabolism

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER3O-419.
YEAST:MONOMER3O-419.
ReactomeiR-SCE-1660661. Sphingolipid de novo biosynthesis.

Chemistry databases

SwissLipidsiSLP:000000932.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrosphingosine 1-phosphate phosphatase LCB3 (EC:3.1.3.-)
Alternative name(s):
Long-chain base protein 3
Sphingolipid resistance protein 2
Gene namesi
Name:LCB3
Synonyms:LBP1, YSR2
Ordered Locus Names:YJL134W
ORF Names:J0671
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJL134W.
SGDiS000003670. LCB3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 86LumenalAdd BLAST86
Transmembranei87 – 107Helical; Name=1Add BLAST21
Topological domaini108 – 112Cytoplasmic5
Transmembranei113 – 133Helical; Name=2Add BLAST21
Topological domaini134 – 182LumenalAdd BLAST49
Transmembranei183 – 203Helical; Name=3Add BLAST21
Topological domaini204 – 207Cytoplasmic4
Transmembranei208 – 228Helical; Name=4Add BLAST21
Topological domaini229 – 245LumenalAdd BLAST17
Transmembranei246 – 266Helical; Name=5Add BLAST21
Topological domaini267 – 276Cytoplasmic10
Transmembranei277 – 297Helical; Name=6Add BLAST21
Topological domaini298 – 315LumenalAdd BLAST18
Transmembranei316 – 336Helical; Name=7Add BLAST21
Topological domaini337 – 384CytoplasmicAdd BLAST48
Transmembranei385 – 405Helical; Name=8Add BLAST21
Topological domaini406 – 409Lumenal4

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi128K → A: Impairs dihydrosphingosine 1-phosphate phosphatase activity. 1 Publication1
Mutagenesisi160H → A: Impairs dihydrosphingosine 1-phosphate phosphatase activity. 1 Publication1
Mutagenesisi210H → A: Impairs dihydrosphingosine 1-phosphate phosphatase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002030371 – 409Dihydrosphingosine 1-phosphate phosphatase LCB3Add BLAST409

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei16PhosphothreonineCombined sources1
Modified residuei18PhosphoserineCombined sources1

Post-translational modificationi

Glycosylated.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP47013.
PRIDEiP47013.

PTM databases

iPTMnetiP47013.

Interactioni

Protein-protein interaction databases

BioGridi33622. 77 interactors.
DIPiDIP-5685N.
IntActiP47013. 4 interactors.
MINTiMINT-572017.

Structurei

3D structure databases

ProteinModelPortaliP47013.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000017322.
HOGENOMiHOG000066080.
InParanoidiP47013.
KOiK04716.
OMAiLMGSHTF.
OrthoDBiEOG092C3MDD.

Family and domain databases

Gene3Di1.20.144.10. 1 hit.
InterProiIPR000326. P_Acid_Pase_2/haloperoxidase.
[Graphical view]
PfamiPF01569. PAP2. 1 hit.
[Graphical view]
SMARTiSM00014. acidPPc. 1 hit.
[Graphical view]
SUPFAMiSSF48317. SSF48317. 1 hit.

Sequencei

Sequence statusi: Complete.

P47013-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDGLNTSNI RKRARTLSNP NDFQEPNYLL DPGNHPSDHF RTRMSKFRFN
60 70 80 90 100
IREKLLVFTN NQSFTLSRWQ KKYRSAFNDL YFTYTSLMGS HTFYVLCLPM
110 120 130 140 150
PVWFGYFETT KDMVYILGYS IYLSGFFKDY WCLPRPRAPP LHRITLSEYT
160 170 180 190 200
TKEYGAPSSH TANATGVSLL FLYNIWRMQE SSVMVQLLLS CVVLFYYMTL
210 220 230 240 250
VFGRIYCGMH GILDLVSGGL IGIVCFIVRM YFKYRFPGLR IEEHWWFPLF
260 270 280 290 300
SVGWGLLLLF KHVKPVDECP CFQDSVAFMG VVSGIECCDW LGKVFGVTLV
310 320 330 340 350
YNLEPNCGWR LTLARLLVGL PCVVIWKYVI SKPMIYTLLI KVFHLKDDRN
360 370 380 390 400
VAARKRLEAT HKEGASKYEC PLYIGEPKID ILGRFIIYAG VPFTVVMCSP

VLFSLLNIA
Length:409
Mass (Da):47,372
Last modified:February 1, 1996 - v1
Checksum:iE0CD7D0F169447C3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87371 Genomic DNA. Translation: CAA60821.1.
Z49410 Genomic DNA. Translation: CAA89430.1.
BK006943 Genomic DNA. Translation: DAA08666.1.
PIRiS55178.
RefSeqiNP_012401.1. NM_001181567.1.

Genome annotation databases

EnsemblFungiiYJL134W; YJL134W; YJL134W.
GeneIDi853307.
KEGGisce:YJL134W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87371 Genomic DNA. Translation: CAA60821.1.
Z49410 Genomic DNA. Translation: CAA89430.1.
BK006943 Genomic DNA. Translation: DAA08666.1.
PIRiS55178.
RefSeqiNP_012401.1. NM_001181567.1.

3D structure databases

ProteinModelPortaliP47013.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33622. 77 interactors.
DIPiDIP-5685N.
IntActiP47013. 4 interactors.
MINTiMINT-572017.

Chemistry databases

SwissLipidsiSLP:000000932.

PTM databases

iPTMnetiP47013.

Proteomic databases

MaxQBiP47013.
PRIDEiP47013.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJL134W; YJL134W; YJL134W.
GeneIDi853307.
KEGGisce:YJL134W.

Organism-specific databases

EuPathDBiFungiDB:YJL134W.
SGDiS000003670. LCB3.

Phylogenomic databases

GeneTreeiENSGT00390000017322.
HOGENOMiHOG000066080.
InParanoidiP47013.
KOiK04716.
OMAiLMGSHTF.
OrthoDBiEOG092C3MDD.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER3O-419.
YEAST:MONOMER3O-419.
ReactomeiR-SCE-1660661. Sphingolipid de novo biosynthesis.

Miscellaneous databases

PROiP47013.

Family and domain databases

Gene3Di1.20.144.10. 1 hit.
InterProiIPR000326. P_Acid_Pase_2/haloperoxidase.
[Graphical view]
PfamiPF01569. PAP2. 1 hit.
[Graphical view]
SMARTiSM00014. acidPPc. 1 hit.
[Graphical view]
SUPFAMiSSF48317. SSF48317. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDS1P1_YEAST
AccessioniPrimary (citable) accession number: P47013
Secondary accession number(s): D6VW50
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 2, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.