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Protein

Glycogenin-2

Gene

GLG2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Self-glucosylating initiator of glycogen synthesis. Catalyzes the formation of a short alpha (1,4)-glucosyl chain covalently attached via a glucose 1-O-tyrosyl linkage to internal tyrosine residues. These chains act as primers for the elongation reaction catalyzed by glycogen synthase. Capable of transferring glucosyl residues to unbound acceptors such as free oligoglucans or oligoglucan derivatives.3 Publications

Catalytic activityi

UDP-alpha-D-glucose + glycogenin = UDP + alpha-D-glucosylglycogenin.

GO - Molecular functioni

  • glycogenin glucosyltransferase activity Source: SGD

GO - Biological processi

  • glycogen biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glycogen biosynthesis

Enzyme and pathway databases

BioCyciYEAST:MONOMER3O-4031.
ReactomeiR-SCE-3322077. Glycogen synthesis.
R-SCE-5357572. Lysosomal glycogen catabolism.
R-SCE-70221. Glycogen breakdown (glycogenolysis).

Protein family/group databases

CAZyiGT8. Glycosyltransferase Family 8.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycogenin-2 (EC:2.4.1.186)
Alternative name(s):
Glycogen synthesis initiator protein 2
Glycogenin glucosyltransferase 2
Gene namesi
Name:GLG2
Ordered Locus Names:YJL137C
ORF Names:J0663
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJL137C.
SGDiS000003673. GLG2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi230 – 2301Y → F: Eliminates glycogen accumulation; when associated with F-232 and F-367. 1 Publication
Mutagenesisi232 – 2321Y → F: Eliminates glycogen accumulation; when associated with F-230 and F-367. 1 Publication
Mutagenesisi367 – 3671Y → F: Eliminates glycogen accumulation; when associated with F-230 and F-232. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 380380Glycogenin-2PRO_0000215182Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi230 – 2301O-linked (Glc...)
Glycosylationi232 – 2321O-linked (Glc...)
Glycosylationi367 – 3671O-linked (Glc...)Curated

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP47011.

Interactioni

Subunit structurei

Interacts with glycogen synthase GSY2.1 Publication

Protein-protein interaction databases

BioGridi33620. 23 interactions.
DIPiDIP-1345N.
IntActiP47011. 3 interactions.
MINTiMINT-387993.

Structurei

3D structure databases

ProteinModelPortaliP47011.
SMRiP47011. Positions 6-258.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000004721.
InParanoidiP47011.
KOiK00750.
OMAiELYSECH.
OrthoDBiEOG092C395N.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR002495. Glyco_trans_8.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF01501. Glyco_transf_8. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

P47011-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKKVAICTL LYSRDYLPGA LTLAYQLQKL LKHAVVEDEI TLCLLIEKKL
60 70 80 90 100
FGDEFKPQEI ALIRSLFKEI IIIEPLKDQE KSIEKNKANL ELLKRPELSH
110 120 130 140 150
TLLKARLWEL VQFDQVLFLD ADTLPLNKEF FEILRLYPEQ TRFQIAAVPD
160 170 180 190 200
IGWPDMFNTG VLLLIPDLDM ATSLQDFLIK TVSIDGADQG IFNQFFNPIC
210 220 230 240 250
NYSKEVLHKV SPLMEWIRLP FTYNVTMPNY GYQSSPAMNF FQQHIRLIHF
260 270 280 290 300
IGTFKPWSRN TTDYDDHYYQ LWRSTQRELY SECHLSNYFT HLQLGNIETE
310 320 330 340 350
TNFYHEPPCL QDLLNHGKRE NQKHVDLDIT SVDRNASQKS TAEKHDIEKP
360 370 380
TSKPQSAFKF DWESTDYLDR VQRAFPKPDT
Length:380
Mass (Da):44,546
Last modified:February 1, 1996 - v1
Checksum:i36BDF556DEF397C0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti241 – 2411Missing AA sequence (PubMed:8900126).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U25436 Genomic DNA. Translation: AAA91644.1.
X87371 Genomic DNA. Translation: CAA60818.1.
Z49412 Genomic DNA. Translation: CAA89432.1.
BK006943 Genomic DNA. Translation: DAA08663.1.
PIRiS55176.
RefSeqiNP_012398.1. NM_001181570.1.

Genome annotation databases

EnsemblFungiiYJL137C; YJL137C; YJL137C.
GeneIDi853304.
KEGGisce:YJL137C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U25436 Genomic DNA. Translation: AAA91644.1.
X87371 Genomic DNA. Translation: CAA60818.1.
Z49412 Genomic DNA. Translation: CAA89432.1.
BK006943 Genomic DNA. Translation: DAA08663.1.
PIRiS55176.
RefSeqiNP_012398.1. NM_001181570.1.

3D structure databases

ProteinModelPortaliP47011.
SMRiP47011. Positions 6-258.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33620. 23 interactions.
DIPiDIP-1345N.
IntActiP47011. 3 interactions.
MINTiMINT-387993.

Protein family/group databases

CAZyiGT8. Glycosyltransferase Family 8.

Proteomic databases

MaxQBiP47011.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJL137C; YJL137C; YJL137C.
GeneIDi853304.
KEGGisce:YJL137C.

Organism-specific databases

EuPathDBiFungiDB:YJL137C.
SGDiS000003673. GLG2.

Phylogenomic databases

GeneTreeiENSGT00390000004721.
InParanoidiP47011.
KOiK00750.
OMAiELYSECH.
OrthoDBiEOG092C395N.

Enzyme and pathway databases

BioCyciYEAST:MONOMER3O-4031.
ReactomeiR-SCE-3322077. Glycogen synthesis.
R-SCE-5357572. Lysosomal glycogen catabolism.
R-SCE-70221. Glycogen breakdown (glycogenolysis).

Miscellaneous databases

PROiP47011.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR002495. Glyco_trans_8.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF01501. Glyco_transf_8. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGLG2_YEAST
AccessioniPrimary (citable) accession number: P47011
Secondary accession number(s): D6VW47
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: September 7, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 981 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.