Phosphatidylinositol transfer protein SFH5 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P47008 (SFH5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 88. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Phosphatidylinositol transfer protein SFH5
Short name=PITP SFH5

Alternative name(s):
SEC14 homolog 5

Gene names

Name:	SFH5
Ordered Locus Names:	YJL145W
ORF Names:	J0644

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	294 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Non-classical phosphatidylinositol (PtdIns) transfer protein (PITP), which exhibits PtdIns-binding/transfer activity in the absence of detectable PtdCho-binding/transfer activity. Regulates PtdIns(4,5)P2 homeostasis at the plasma membrane. Ref.5 Ref.6 Ref.9
Subcellular location	Cytoplasm. Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein Ref.5 Ref.6 Ref.7 Ref.9.
Miscellaneous	Present with 6540 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the SFH5 family. Contains 1 CRAL-TRIO domain.

Ontologies

Keywords
Biological process	Lipid transport Transport
Cellular component	Cytoplasm Endoplasmic reticulum Membrane Microsome
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Golgi to plasma membrane protein transport Inferred from genetic interaction Ref.5. Source: SGD phosphatidylinositol metabolic process Inferred from genetic interaction PubMed 16262726. Source: SGD regulation of establishment of cell polarity Inferred from genetic interaction PubMed 19477927. Source: SGD regulation of exocytosis Inferred from genetic interaction PubMed 19477927. Source: SGD
Cellular_component	cortical endoplasmic reticulum Inferred from direct assay PubMed 16262726. Source: SGD cytosol Inferred from direct assay Ref.6 PubMed 19477927. Source: SGD endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from direct assay PubMed 22842922. Source: SGD plasma membrane Inferred from direct assay PubMed 19477927. Source: SGD
Molecular_function	phosphatidylinositol transporter activity Inferred from direct assay Ref.5. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 294

294

Phosphatidylinositol transfer protein SFH5

PRO_0000203033

Regions

Domain

100 – 266

167

CRAL-TRIO

Experimental info

Sequence conflict

F → S in AAS56909. Ref.4

Sequences

Sequence

Length

Mass (Da)

Tools

P47008 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: E75B80F3AB56EF70
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FASTA

294

34,388

        10         20         30         40         50         60 
MKFDNDSEKQ VFDKLKKAIP GIIKEKCAGY DELYGYKLNP EGLTQEEVDK YYDEKIADRL 

        70         80         90        100        110        120 
TYKLCKAYQF EYSTIVQNLI DILNWRREFN PLSCAYKEVH NTELQNVGIL TFDANGDANK 

       130        140        150        160        170        180 
KAVTWNLYGQ LVKKKELFQN VDKFVRYRIG LMEKGLSLLD FTSSDNNYMT QVHDYKGVSV 

       190        200        210        220        230        240 
WRMDSDIKNC SKTVIGIFQK YYPELLYAKY FVNVPTVFGW VYDLIKKFVD ETTRKKFVVL 

       250        260        270        280        290 
TDGSKLGQYL KDCPYEGYGG KDKKNNLTKQ NVTNVHPTEY GLYILQKQII EDVE

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of a 40.7 kb segment from the left arm of yeast chromosome X reveals 14 known genes and 13 new open reading frames including homologues of genes clustered on the right arm of chromosome XI." Katsoulou C., Tzermia M., Tavernarakis N., Alexandraki D. Yeast 12:787-797(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Identification of a novel family of nonclassic yeast phosphatidylinositol transfer proteins whose function modulates phospholipase D activity and Sec14p-independent cell growth." Li X., Routt S.M., Xie Z., Cui X., Fang M., Kearns M.A., Bard M., Kirsch D.R., Bankaitis V.A. Mol. Biol. Cell 11:1989-2005(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, PHOSPHATIDYLINOSITOL-BINDING, SUBCELLULAR LOCATION.
[6]	"Subcellular localization of yeast Sec14 homologues and their involvement in regulation of phospholipid turnover." Schnabl M., Oskolkova O.V., Holic R., Brezna B., Pichler H., Zagorsek M., Kohlwein S.D., Paltauf F., Daum G., Griac P. Eur. J. Biochem. 270:3133-3145(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"Sec14p-like proteins regulate phosphoinositide homoeostasis and intracellular protein and lipid trafficking in yeast." Mousley C.J., Tyeryar K.R., Ryan M.M., Bankaitis V.A. Biochem. Soc. Trans. 34:346-350(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X87371 Genomic DNA. Translation: CAA60810.1. Z49420 Genomic DNA. Translation: CAA89440.1. AY558583 Genomic DNA. Translation: AAS56909.1. BK006943 Genomic DNA. Translation: DAA08656.1.
PIR	S55168.
RefSeq	NP_012390.1. NM_001181578.1.
3D structure databases
ProteinModelPortal	P47008.
ModBase	Search...
Protein-protein interaction databases
IntAct	P47008. 1 interaction.
MINT	MINT-4491120.
STRING	4932.YJL145W.
Proteomic databases
PaxDb	P47008.
PeptideAtlas	P47008.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJL145W; YJL145W; YJL145W.
GeneID	853296.
KEGG	sce:YJL145W.
Organism-specific databases
CYGD	YJL145w.
SGD	S000003681. SFH5.
Phylogenomic databases
eggNOG	NOG270325.
HOGENOM	HOG000048479.
OMA	RIGLMEK.
OrthoDB	EOG41ZJKJ.
Gene expression databases
Genevestigator	P47008.
GermOnline	YJL145W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.40.525.10. 1 hit.
InterPro	IPR001251. CRAL-TRIO_dom. IPR011074. CRAL/TRIO_N_dom. [Graphical view]
Pfam	PF00650. CRAL_TRIO. 1 hit. PF03765. CRAL_TRIO_N. 1 hit. [Graphical view]
SMART	SM00516. SEC14. 1 hit. [Graphical view]
SUPFAM	SSF52087. CRAL_TRIO_C. 1 hit.
PROSITE	PS50191. CRAL_TRIO. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	973609.

Entry information

Entry name

SFH5_YEAST

Accession

Primary (citable) accession number: P47008
Secondary accession number(s): D6VW40, Q6Q535

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	April 3, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents