Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Phosphatidylinositol transfer protein SFH5

Gene

SFH5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Non-classical phosphatidylinositol (PtdIns) transfer protein (PITP), which exhibits PtdIns-binding/transfer activity in the absence of detectable PtdCho-binding/transfer activity. Regulates PtdIns(4,5)P2 homeostasis at the plasma membrane.3 Publications

GO - Molecular functioni

  • phosphatidylinositol transporter activity Source: SGD

GO - Biological processi

  • Golgi to plasma membrane protein transport Source: SGD
  • phosphatidylinositol metabolic process Source: SGD
  • phospholipid transport Source: SGD
  • regulation of establishment of cell polarity Source: SGD
  • regulation of exocytosis Source: SGD
Complete GO annotation...

Keywords - Biological processi

Lipid transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31589-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol transfer protein SFH5
Short name:
PITP SFH5
Alternative name(s):
SEC14 homolog 5
Gene namesi
Name:SFH5
Ordered Locus Names:YJL145W
ORF Names:J0644
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome X

Organism-specific databases

CYGDiYJL145w.
EuPathDBiFungiDB:YJL145W.
SGDiS000003681. SFH5.

Subcellular locationi

GO - Cellular componenti

  • cell periphery Source: SGD
  • cortical endoplasmic reticulum Source: SGD
  • cytosol Source: SGD
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 294294Phosphatidylinositol transfer protein SFH5PRO_0000203033Add
BLAST

Proteomic databases

MaxQBiP47008.
PaxDbiP47008.
PeptideAtlasiP47008.

Interactioni

Protein-protein interaction databases

BioGridi33613. 19 interactions.
IntActiP47008. 2 interactions.
MINTiMINT-4491120.

Structurei

3D structure databases

ProteinModelPortaliP47008.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini100 – 266167CRAL-TRIOPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the SFH5 family.Curated
Contains 1 CRAL-TRIO domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG270325.
HOGENOMiHOG000048479.
InParanoidiP47008.
OMAiQVHDYNN.
OrthoDBiEOG7VQJQ2.

Family and domain databases

Gene3Di3.40.525.10. 1 hit.
InterProiIPR001251. CRAL-TRIO_dom.
IPR011074. CRAL/TRIO_N_dom.
[Graphical view]
PfamiPF00650. CRAL_TRIO. 1 hit.
PF03765. CRAL_TRIO_N. 1 hit.
[Graphical view]
SMARTiSM00516. SEC14. 1 hit.
[Graphical view]
SUPFAMiSSF52087. SSF52087. 1 hit.
PROSITEiPS50191. CRAL_TRIO. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47008-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFDNDSEKQ VFDKLKKAIP GIIKEKCAGY DELYGYKLNP EGLTQEEVDK
60 70 80 90 100
YYDEKIADRL TYKLCKAYQF EYSTIVQNLI DILNWRREFN PLSCAYKEVH
110 120 130 140 150
NTELQNVGIL TFDANGDANK KAVTWNLYGQ LVKKKELFQN VDKFVRYRIG
160 170 180 190 200
LMEKGLSLLD FTSSDNNYMT QVHDYKGVSV WRMDSDIKNC SKTVIGIFQK
210 220 230 240 250
YYPELLYAKY FVNVPTVFGW VYDLIKKFVD ETTRKKFVVL TDGSKLGQYL
260 270 280 290
KDCPYEGYGG KDKKNNLTKQ NVTNVHPTEY GLYILQKQII EDVE
Length:294
Mass (Da):34,388
Last modified:February 1, 1996 - v1
Checksum:iE75B80F3AB56EF70
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti70 – 701F → S in AAS56909 (PubMed:17322287).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87371 Genomic DNA. Translation: CAA60810.1.
Z49420 Genomic DNA. Translation: CAA89440.1.
AY558583 Genomic DNA. Translation: AAS56909.1.
BK006943 Genomic DNA. Translation: DAA08656.1.
PIRiS55168.
RefSeqiNP_012390.1. NM_001181578.1.

Genome annotation databases

EnsemblFungiiYJL145W; YJL145W; YJL145W.
GeneIDi853296.
KEGGisce:YJL145W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87371 Genomic DNA. Translation: CAA60810.1.
Z49420 Genomic DNA. Translation: CAA89440.1.
AY558583 Genomic DNA. Translation: AAS56909.1.
BK006943 Genomic DNA. Translation: DAA08656.1.
PIRiS55168.
RefSeqiNP_012390.1. NM_001181578.1.

3D structure databases

ProteinModelPortaliP47008.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33613. 19 interactions.
IntActiP47008. 2 interactions.
MINTiMINT-4491120.

Proteomic databases

MaxQBiP47008.
PaxDbiP47008.
PeptideAtlasiP47008.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJL145W; YJL145W; YJL145W.
GeneIDi853296.
KEGGisce:YJL145W.

Organism-specific databases

CYGDiYJL145w.
EuPathDBiFungiDB:YJL145W.
SGDiS000003681. SFH5.

Phylogenomic databases

eggNOGiNOG270325.
HOGENOMiHOG000048479.
InParanoidiP47008.
OMAiQVHDYNN.
OrthoDBiEOG7VQJQ2.

Enzyme and pathway databases

BioCyciYEAST:G3O-31589-MONOMER.

Miscellaneous databases

NextBioi973609.
PROiP47008.

Family and domain databases

Gene3Di3.40.525.10. 1 hit.
InterProiIPR001251. CRAL-TRIO_dom.
IPR011074. CRAL/TRIO_N_dom.
[Graphical view]
PfamiPF00650. CRAL_TRIO. 1 hit.
PF03765. CRAL_TRIO_N. 1 hit.
[Graphical view]
SMARTiSM00516. SEC14. 1 hit.
[Graphical view]
SUPFAMiSSF52087. SSF52087. 1 hit.
PROSITEiPS50191. CRAL_TRIO. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of a 40.7 kb segment from the left arm of yeast chromosome X reveals 14 known genes and 13 new open reading frames including homologues of genes clustered on the right arm of chromosome XI."
    Katsoulou C., Tzermia M., Tavernarakis N., Alexandraki D.
    Yeast 12:787-797(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Identification of a novel family of nonclassic yeast phosphatidylinositol transfer proteins whose function modulates phospholipase D activity and Sec14p-independent cell growth."
    Li X., Routt S.M., Xie Z., Cui X., Fang M., Kearns M.A., Bard M., Kirsch D.R., Bankaitis V.A.
    Mol. Biol. Cell 11:1989-2005(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHATIDYLINOSITOL-BINDING, SUBCELLULAR LOCATION.
  6. "Subcellular localization of yeast Sec14 homologues and their involvement in regulation of phospholipid turnover."
    Schnabl M., Oskolkova O.V., Holic R., Brezna B., Pichler H., Zagorsek M., Kohlwein S.D., Paltauf F., Daum G., Griac P.
    Eur. J. Biochem. 270:3133-3145(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Sec14p-like proteins regulate phosphoinositide homoeostasis and intracellular protein and lipid trafficking in yeast."
    Mousley C.J., Tyeryar K.R., Ryan M.M., Bankaitis V.A.
    Biochem. Soc. Trans. 34:346-350(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiSFH5_YEAST
AccessioniPrimary (citable) accession number: P47008
Secondary accession number(s): D6VW40, Q6Q535
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: July 22, 2015
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6540 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.