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P47008 (SFH5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphatidylinositol transfer protein SFH5

Short name=PITP SFH5
Alternative name(s):
SEC14 homolog 5
Gene names
Name:SFH5
Ordered Locus Names:YJL145W
ORF Names:J0644
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length294 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Non-classical phosphatidylinositol (PtdIns) transfer protein (PITP), which exhibits PtdIns-binding/transfer activity in the absence of detectable PtdCho-binding/transfer activity. Regulates PtdIns(4,5)P2 homeostasis at the plasma membrane. Ref.5 Ref.6 Ref.9

Subcellular location

Cytoplasm. Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein Ref.5 Ref.6 Ref.7 Ref.9.

Miscellaneous

Present with 6540 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the SFH5 family.

Contains 1 CRAL-TRIO domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 294294Phosphatidylinositol transfer protein SFH5
PRO_0000203033

Regions

Domain100 – 266167CRAL-TRIO

Experimental info

Sequence conflict701F → S in AAS56909. Ref.4

Sequences

Sequence LengthMass (Da)Tools
P47008 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: E75B80F3AB56EF70

FASTA29434,388
        10         20         30         40         50         60 
MKFDNDSEKQ VFDKLKKAIP GIIKEKCAGY DELYGYKLNP EGLTQEEVDK YYDEKIADRL 

        70         80         90        100        110        120 
TYKLCKAYQF EYSTIVQNLI DILNWRREFN PLSCAYKEVH NTELQNVGIL TFDANGDANK 

       130        140        150        160        170        180 
KAVTWNLYGQ LVKKKELFQN VDKFVRYRIG LMEKGLSLLD FTSSDNNYMT QVHDYKGVSV 

       190        200        210        220        230        240 
WRMDSDIKNC SKTVIGIFQK YYPELLYAKY FVNVPTVFGW VYDLIKKFVD ETTRKKFVVL 

       250        260        270        280        290 
TDGSKLGQYL KDCPYEGYGG KDKKNNLTKQ NVTNVHPTEY GLYILQKQII EDVE 

« Hide

References

« Hide 'large scale' references
[1]"Sequence analysis of a 40.7 kb segment from the left arm of yeast chromosome X reveals 14 known genes and 13 new open reading frames including homologues of genes clustered on the right arm of chromosome XI."
Katsoulou C., Tzermia M., Tavernarakis N., Alexandraki D.
Yeast 12:787-797(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[2]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Identification of a novel family of nonclassic yeast phosphatidylinositol transfer proteins whose function modulates phospholipase D activity and Sec14p-independent cell growth."
Li X., Routt S.M., Xie Z., Cui X., Fang M., Kearns M.A., Bard M., Kirsch D.R., Bankaitis V.A.
Mol. Biol. Cell 11:1989-2005(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, PHOSPHATIDYLINOSITOL-BINDING, SUBCELLULAR LOCATION.
[6]"Subcellular localization of yeast Sec14 homologues and their involvement in regulation of phospholipid turnover."
Schnabl M., Oskolkova O.V., Holic R., Brezna B., Pichler H., Zagorsek M., Kohlwein S.D., Paltauf F., Daum G., Griac P.
Eur. J. Biochem. 270:3133-3145(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[7]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"Sec14p-like proteins regulate phosphoinositide homoeostasis and intracellular protein and lipid trafficking in yeast."
Mousley C.J., Tyeryar K.R., Ryan M.M., Bankaitis V.A.
Biochem. Soc. Trans. 34:346-350(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X87371 Genomic DNA. Translation: CAA60810.1.
Z49420 Genomic DNA. Translation: CAA89440.1.
AY558583 Genomic DNA. Translation: AAS56909.1.
BK006943 Genomic DNA. Translation: DAA08656.1.
PIRS55168.
RefSeqNP_012390.1. NM_001181578.1.

3D structure databases

ProteinModelPortalP47008.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33613. 19 interactions.
IntActP47008. 2 interactions.
MINTMINT-4491120.
STRING4932.YJL145W.

Proteomic databases

PaxDbP47008.
PeptideAtlasP47008.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJL145W; YJL145W; YJL145W.
GeneID853296.
KEGGsce:YJL145W.

Organism-specific databases

CYGDYJL145w.
SGDS000003681. SFH5.

Phylogenomic databases

eggNOGNOG270325.
HOGENOMHOG000048479.
OMAQVHDYNN.
OrthoDBEOG7VQJQ2.

Enzyme and pathway databases

BioCycYEAST:G3O-31589-MONOMER.

Gene expression databases

GenevestigatorP47008.

Family and domain databases

Gene3D3.40.525.10. 1 hit.
InterProIPR001251. CRAL-TRIO_dom.
IPR011074. CRAL/TRIO_N_dom.
[Graphical view]
PfamPF00650. CRAL_TRIO. 1 hit.
PF03765. CRAL_TRIO_N. 1 hit.
[Graphical view]
SMARTSM00516. SEC14. 1 hit.
[Graphical view]
SUPFAMSSF52087. SSF52087. 1 hit.
PROSITEPS50191. CRAL_TRIO. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio973609.

Entry information

Entry nameSFH5_YEAST
AccessionPrimary (citable) accession number: P47008
Secondary accession number(s): D6VW40, Q6Q535
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: April 16, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families