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P47006

- RPA34_YEAST

UniProt

P47006 - RPA34_YEAST

Protein

DNA-directed RNA polymerase I subunit RPA34

Gene

RPA34

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    DNA-dependent RNA polymerases catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I (Pol I) which synthesizes ribosomal RNA precursors. Besides, RNA polymerase I has intrinsic RNA cleavage activity. The heterodimer formed by RPA34 and RPA49 stimulates transcript elongation by Pol I.5 Publications

    GO - Molecular functioni

    1. RNA polymerase I activity Source: UniProtKB

    GO - Biological processi

    1. ribosome biogenesis Source: UniProtKB-KW
    2. transcription elongation from RNA polymerase I promoter Source: SGD
    3. transcription from RNA polymerase I promoter Source: UniProtKB
    4. transcription of nuclear large rRNA transcript from RNA polymerase I promoter Source: SGD

    Keywords - Biological processi

    Ribosome biogenesis, Transcription

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31592-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA-directed RNA polymerase I subunit RPA34
    Short name:
    A34
    Alternative name(s):
    DNA-directed DNA-dependent RNA polymerase 34.5 kDa polypeptide
    Short name:
    A34.5
    Gene namesi
    Name:RPA34
    Ordered Locus Names:YJL148W
    ORF Names:J0637
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome X

    Organism-specific databases

    CYGDiYJL148w.
    SGDiS000003684. RPA34.

    Subcellular locationi

    Nucleusnucleolus 1 Publication

    GO - Cellular componenti

    1. DNA-directed RNA polymerase I complex Source: UniProtKB
    2. nucleolus Source: SGD
    3. nucleus Source: SGD

    Keywords - Cellular componenti

    DNA-directed RNA polymerase, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 233233DNA-directed RNA polymerase I subunit RPA34PRO_0000203031Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei10 – 101Phosphoserine3 Publications
    Modified residuei12 – 121Phosphoserine3 Publications
    Modified residuei14 – 141Phosphoserine4 Publications
    Modified residuei60 – 601Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP47006.
    PaxDbiP47006.
    PeptideAtlasiP47006.

    Expressioni

    Gene expression databases

    GenevestigatoriP47006.

    Interactioni

    Subunit structurei

    Component of the RNA polymerase I (Pol I) complex consisting of 14 subunits: RPA135, RPA190, RPC40, RPA14, RPB5, RPO26, RPA43, RPB8, RPA12, RPB10, RPC19, RPC10, RPA49 and RPA34. The complex is composed of a horseshoe-shaped core containing ten subunits (RPA135, RPA190, RPB5, RPO26, RPB8, RPB10, RPC10, RPA12, RPC19 and RPC40) where RPA135 and RPA190 form the DNA-binding cleft. Outside of the core, RPA14 and RPA43 form the stalk that mediates interactions with transcription initiation factors and newly synthesized RNA. Forms a TFIIF-like heterodimer with RPA49; the heterodimer formed by RPA34 and RPA49 can be dissociated from the Pol I core giving rise to a 12 subunit form A* of Pol I (formerly called pol A) that shows impaired transcript elongation activity and increased sensitivity to alpha-amanitin. The heterodimer formed by RPA34 and RPA49 stabilizes subunit RPA12 and stimulates RPA12-dependent RNA cleavage.8 Publications

    Protein-protein interaction databases

    BioGridi33610. 322 interactions.
    DIPiDIP-7973N.
    IntActiP47006. 27 interactions.
    MINTiMINT-2732801.
    STRINGi4932.YJL148W.

    Structurei

    Secondary structure

    1
    233
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi29 – 313
    Beta strandi52 – 598
    Turni64 – 663
    Beta strandi68 – 714
    Beta strandi74 – 763
    Beta strandi79 – 824
    Beta strandi85 – 917
    Helixi93 – 964
    Beta strandi99 – 1013
    Beta strandi106 – 1127
    Beta strandi116 – 1216
    Beta strandi125 – 1273
    Beta strandi134 – 1418
    Helixi150 – 1534
    Helixi177 – 1793

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4C2MX-ray2.803/N1-233[»]
    4C3HX-ray3.27N1-233[»]
    4C3IX-ray3.0N1-233[»]
    4C3JX-ray3.35N1-233[»]
    ProteinModelPortaliP47006.
    SMRiP47006. Positions 25-181.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi184 – 23350Glu/Lys-richAdd
    BLAST
    Compositional biasi208 – 2158Poly-Lys
    Compositional biasi221 – 23010Poly-Lys

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiNOG39958.
    KOiK03003.
    OMAiRCAINIC.
    OrthoDBiEOG7CK3K9.

    Family and domain databases

    InterProiIPR013240. DNA-dir_RNA_pol1_su_RPA34.
    [Graphical view]
    PfamiPF08208. RNA_polI_A34. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P47006-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSKLSKDYVS DSDSDDEVIS NEFSIPDGFK KCKHLKNFPL NGDNKKKAKQ    50
    QQVWLIKFPS NVDISKLKSL PVDFESSTTM TIDKHDYKIM DDTDIESSLT 100
    QDNLSNMTLL VPSESKESLK IASTAKDNAP LQFDKVFSVS ETAKIPAIDY 150
    SKVRVPRKDV PKVEGLKLEH FATGYDAEDF HVAEEVKENK KEPKKRSHHD 200
    DEEESSEKKK KKKEKREKRE KKDKKDKKKK HRD 233
    Length:233
    Mass (Da):26,875
    Last modified:February 1, 1996 - v1
    Checksum:iDCD42DFEC574EDB6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49423 Genomic DNA. Translation: CAA89443.1.
    X87371 Genomic DNA. Translation: CAA60807.1.
    BK006943 Genomic DNA. Translation: DAA08653.1.
    PIRiS55165.
    RefSeqiNP_012387.1. NM_001181581.1.

    Genome annotation databases

    EnsemblFungiiYJL148W; YJL148W; YJL148W.
    GeneIDi853293.
    KEGGisce:YJL148W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49423 Genomic DNA. Translation: CAA89443.1 .
    X87371 Genomic DNA. Translation: CAA60807.1 .
    BK006943 Genomic DNA. Translation: DAA08653.1 .
    PIRi S55165.
    RefSeqi NP_012387.1. NM_001181581.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4C2M X-ray 2.80 3/N 1-233 [» ]
    4C3H X-ray 3.27 N 1-233 [» ]
    4C3I X-ray 3.0 N 1-233 [» ]
    4C3J X-ray 3.35 N 1-233 [» ]
    ProteinModelPortali P47006.
    SMRi P47006. Positions 25-181.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33610. 322 interactions.
    DIPi DIP-7973N.
    IntActi P47006. 27 interactions.
    MINTi MINT-2732801.
    STRINGi 4932.YJL148W.

    Proteomic databases

    MaxQBi P47006.
    PaxDbi P47006.
    PeptideAtlasi P47006.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YJL148W ; YJL148W ; YJL148W .
    GeneIDi 853293.
    KEGGi sce:YJL148W.

    Organism-specific databases

    CYGDi YJL148w.
    SGDi S000003684. RPA34.

    Phylogenomic databases

    eggNOGi NOG39958.
    KOi K03003.
    OMAi RCAINIC.
    OrthoDBi EOG7CK3K9.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31592-MONOMER.

    Miscellaneous databases

    NextBioi 973600.

    Gene expression databases

    Genevestigatori P47006.

    Family and domain databases

    InterProi IPR013240. DNA-dir_RNA_pol1_su_RPA34.
    [Graphical view ]
    Pfami PF08208. RNA_polI_A34. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of a 40.7 kb segment from the left arm of yeast chromosome X reveals 14 known genes and 13 new open reading frames including homologues of genes clustered on the right arm of chromosome XI."
      Katsoulou C., Tzermia M., Tavernarakis N., Alexandraki D.
      Yeast 12:787-797(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "A34.5, a nonessential component of yeast RNA polymerase I, cooperates with subunit A14 and DNA topoisomerase I to produce a functional rRNA synthesis machine."
      Gadal O., Mariotte-Labarre S., Chedin S., Quemeneur E., Carles C., Sentenac A., Thuriaux P.
      Mol. Cell. Biol. 17:1787-1795(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION IN THE RNA POL I COMPLEX, PHOSPHORYLATION.
    5. "Differential roles of phosphorylation in the formation of transcriptional active RNA polymerase I."
      Fath S., Milkereit P., Peyroche G., Riva M., Carles C., Tschochner H.
      Proc. Natl. Acad. Sci. U.S.A. 98:14334-14339(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE RNA POL I COMPLEX.
    6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-10; SER-12 AND SER-14, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-14 AND SER-60, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-10; SER-12 AND SER-14, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "RNA polymerase I contains a TFIIF-related DNA-binding subcomplex."
      Geiger S.R., Lorenzen K., Schreieck A., Hanecker P., Kostrewa D., Heck A.J., Cramer P.
      Mol. Cell 39:583-594(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH RPA49, IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT.
    10. "Localization of the yeast RNA polymerase I-specific subunits."
      Bischler N., Brino L., Carles C., Riva M., Tschochner H., Mallouh V., Schultz P.
      EMBO J. 21:4136-4144(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: ELECTRON MICROSCOPY OF THE RNA POLYMERASE I COMPLEX.
    11. "Rpa12p, a conserved RNA polymerase I subunit with two functional domains."
      Van Mullem V., Landrieux E., Vandenhaute J., Thuriaux P.
      Mol. Microbiol. 43:1105-1113(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE RNA POL I COMPLEX.
    12. "The A14-A43 heterodimer subunit in yeast RNA pol I and their relationship to Rpb4-Rpb7 pol II subunits."
      Peyroche G., Levillain E., Siaut M., Callebaut I., Schultz P., Sentenac A., Riva M., Carles C.
      Proc. Natl. Acad. Sci. U.S.A. 99:14670-14675(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE RNA POL I COMPLEX.
    13. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    14. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    15. Cited for: STRUCTURE BY ELECTRON MICROSCOPY (12.00 ANGSTROMS) OF THE POL I COMPLEX, FUNCTION, SUBUNIT.
    16. Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF THE POL I COMPLEX, FUNCTION, SUBUNIT.
    17. "RNA polymerase I structure and transcription regulation."
      Engel C., Sainsbury S., Cheung A.C., Kostrewa D., Cramer P.
      Nature 502:650-655(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF THE POL I COMPLEX, FUNCTION, SUBUNIT.

    Entry informationi

    Entry nameiRPA34_YEAST
    AccessioniPrimary (citable) accession number: P47006
    Secondary accession number(s): D6VW37
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 113 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 3360 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3