Reviewed, UniProtKB/Swiss-Prot P47006 (RPA34_YEAST)

Names and origin

Protein names

Recommended name:
    DNA-directed RNA polymerase I subunit RPA34
      Short name=A34
Alternative name(s):
    DNA-directed DNA-dependent RNA polymerase 34.5 kDa polypeptide
      Short name=A34.5

Gene names

Name:	RPA34
Ordered Locus Names:	YJL148W
ORF Names:	J0637

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	233 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Ref.3
Subunit structure	Component of the RNA polymerase I (Pol I) complex consisiting of 14 subunits.
Subcellular location	Nucleus › nucleolus.
Miscellaneous	Present with 3360 molecules/cell in log phase SD medium. Ref.10
Sequence similarities	Belongs to the eukaryotic RPA34 RNA polymerase subunit family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	DNA-directed RNA polymerase I complex Traceable author statement. Source: SGD
Molecular function	DNA-directed RNA polymerase activity Traceable author statement. Source: SGD identical protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-26109,EBI-26109
DBP3	P20447	1	EBI-26109,EBI-5608
HMF1	P40037	1	EBI-26109,EBI-8389
KRE27	P40540	1	EBI-26109,EBI-24977
NHP2	P32495	1	EBI-26109,EBI-12014
NOP10	Q6Q547	1	EBI-26109,EBI-2052475
PRP43	P53131	1	EBI-26109,EBI-505
TMA23	Q03525	1	EBI-26109,EBI-27597

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 233	233	DNA-directed RNA polymerase I subunit RPA34		PRO_0000203031
Regions
	Compositional bias	184 – 233	50	Glu/Lys-rich
	Compositional bias	208 – 215	8	Poly-Lys
	Compositional bias	221 – 230	10	Poly-Lys
Amino acid modifications
	Modified residue	10	1	Phosphoserine Ref.11
	Modified residue	12	1	Phosphoserine Ref.11
	Modified residue	14	1	Phosphoserine Ref.11 Ref.5
	Modified residue	60	1	Phosphoserine Ref.5
	Modified residue	115	1	Phosphoserine Ref.5
	Modified residue	118	1	Phosphoserine Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P47006-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: DCD42DFEC574EDB6	FASTA	233	26,875
	10 20 30 40 50 60 MSKLSKDYVS DSDSDDEVIS NEFSIPDGFK KCKHLKNFPL NGDNKKKAKQ QQVWLIKFPS 70 80 90 100 110 120 NVDISKLKSL PVDFESSTTM TIDKHDYKIM DDTDIESSLT QDNLSNMTLL VPSESKESLK 130 140 150 160 170 180 IASTAKDNAP LQFDKVFSVS ETAKIPAIDY SKVRVPRKDV PKVEGLKLEH FATGYDAEDF 190 200 210 220 230 HVAEEVKENK KEPKKRSHHD DEEESSEKKK KKKEKREKRE KKDKKDKKKK HRD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of a 40.7 kb segment from the left arm of yeast chromosome X reveals 14 known genes and 13 new open reading frames including homologues of genes clustered on the right arm of chromosome XI." Katsoulou C., Tzermia M., Tavernarakis N., Alexandraki D. Yeast 12:787-797(1996) [PubMed: 8813765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"A34.5, a nonessential component of yeast RNA polymerase I, cooperates with subunit A14 and DNA topoisomerase I to produce a functional rRNA synthesis machine." Gadal O., Mariotte-Labarre S., Chedin S., Quemeneur E., Carles C., Sentenac A., Thuriaux P. Mol. Cell. Biol. 17:1787-1795(1997) [PubMed: 9121426] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN THE RNA POL I COMPLEX, PHOSPHORYLATION.
[4]	"Differential roles of phosphorylation in the formation of transcriptional active RNA polymerase I." Fath S., Milkereit P., Peyroche G., Riva M., Carles C., Tschochner H. Proc. Natl. Acad. Sci. U.S.A. 98:14334-14339(2001) [PubMed: 11717393] [Abstract] Cited for: IDENTIFICATION IN THE RNA POL I COMPLEX.
[5]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-14; SER-60; SER-115 AND SER-118, MASS SPECTROMETRY.
[6]	"Localization of the yeast RNA polymerase I-specific subunits." Bischler N., Brino L., Carles C., Riva M., Tschochner H., Mallouh V., Schultz P. EMBO J. 21:4136-4144(2002) [PubMed: 12145213] [Abstract] Cited for: ELECTRON MICROSCOPY OF THE RNA POLYMERASE I COMPLEX.
[7]	"Rpa12p, a conserved RNA polymerase I subunit with two functional domains." Van Mullem V., Landrieux E., Vandenhaute J., Thuriaux P. Mol. Microbiol. 43:1105-1113(2002) [PubMed: 11918799] [Abstract] Cited for: IDENTIFICATION IN THE RNA POL I COMPLEX.
[8]	"The A14-A43 heterodimer subunit in yeast RNA pol I and their relationship to Rpb4-Rpb7 pol II subunits." Peyroche G., Levillain E., Siaut M., Callebaut I., Schultz P., Sentenac A., Riva M., Carles C. Proc. Natl. Acad. Sci. U.S.A. 99:14670-14675(2002) [PubMed: 12407181] [Abstract] Cited for: IDENTIFICATION IN THE RNA POL I COMPLEX.
[9]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[10]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[11]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-10; SER-12 AND SER-14, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z49423 Genomic DNA. Translation: CAA89443.1. X87371 Genomic DNA. Translation: CAA60807.1.
PIR	S55165.
RefSeq	NP_012387.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P47006. 29 interactions.
STRING	P47006.
Proteomic databases
PeptideAtlas	P47006.
PRIDE	P47006.
Genome annotation databases
Ensembl	YJL148W; YJL148W; YJL148W; Saccharomyces cerevisiae. [Genome view]
GeneID	853293.
KEGG	sce:YJL148W.
NMPDR	fig|4932.3.peg.3352.
Organism-specific databases
CYGD	YJL148w.
SGD	S000003684. RPA34.
Phylogenomic databases
HOGENOM	P47006.
OMA	MRHFPTG
OrthoDB	EOG99PCFH
Gene expression databases
ArrayExpress	P47006.
Genevestigator	P47006.
GermOnline	YJL148W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013240. RNA_pol1_su_RPA34. [Graphical view]
Pfam	PF08208. RNA_polI_A34. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	973600.

Entry information

Entry name

RPA34_YEAST

Accession

Primary (citable) accession number: P47006

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	November 24, 2009
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)