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Protein

DNA-directed RNA polymerase I subunit RPA34

Gene

RPA34

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerases catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I (Pol I) which synthesizes ribosomal RNA precursors. Besides, RNA polymerase I has intrinsic RNA cleavage activity. The heterodimer formed by RPA34 and RPA49 stimulates transcript elongation by Pol I.5 Publications

GO - Molecular functioni

  • RNA polymerase I activity Source: UniProtKB

GO - Biological processi

  • ribosome biogenesis Source: UniProtKB-KW
  • transcription elongation from RNA polymerase I promoter Source: SGD
  • transcription from RNA polymerase I promoter Source: UniProtKB
  • transcription of nuclear large rRNA transcript from RNA polymerase I promoter Source: SGD
Complete GO annotation...

Keywords - Biological processi

Ribosome biogenesis, Transcription

Enzyme and pathway databases

BioCyciYEAST:G3O-31592-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase I subunit RPA34
Short name:
A34
Alternative name(s):
DNA-directed DNA-dependent RNA polymerase 34.5 kDa polypeptide
Short name:
A34.5
Gene namesi
Name:RPA34
Ordered Locus Names:YJL148W
ORF Names:J0637
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJL148W.
SGDiS000003684. RPA34.

Subcellular locationi

GO - Cellular componenti

  • DNA-directed RNA polymerase I complex Source: UniProtKB
  • nucleolus Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002030311 – 233DNA-directed RNA polymerase I subunit RPA34Add BLAST233

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei10PhosphoserineCombined sources1
Modified residuei12PhosphoserineCombined sources1
Modified residuei14PhosphoserineCombined sources1
Modified residuei60PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP47006.
PRIDEiP47006.

PTM databases

iPTMnetiP47006.

Interactioni

Subunit structurei

Component of the RNA polymerase I (Pol I) complex consisting of 14 subunits: RPA135, RPA190, RPC40, RPA14, RPB5, RPO26, RPA43, RPB8, RPA12, RPB10, RPC19, RPC10, RPA49 and RPA34. The complex is composed of a horseshoe-shaped core containing ten subunits (RPA135, RPA190, RPB5, RPO26, RPB8, RPB10, RPC10, RPA12, RPC19 and RPC40) where RPA135 and RPA190 form the DNA-binding cleft. Outside of the core, RPA14 and RPA43 form the stalk that mediates interactions with transcription initiation factors and newly synthesized RNA. Forms a TFIIF-like heterodimer with RPA49; the heterodimer formed by RPA34 and RPA49 can be dissociated from the Pol I core giving rise to a 12 subunit form A* of Pol I (formerly called pol A) that shows impaired transcript elongation activity and increased sensitivity to alpha-amanitin. The heterodimer formed by RPA34 and RPA49 stabilizes subunit RPA12 and stimulates RPA12-dependent RNA cleavage.8 Publications

Protein-protein interaction databases

BioGridi33610. 327 interactors.
DIPiDIP-7973N.
IntActiP47006. 29 interactors.
MINTiMINT-2732801.

Structurei

Secondary structure

1233
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi29 – 31Combined sources3
Beta strandi52 – 59Combined sources8
Turni64 – 66Combined sources3
Beta strandi68 – 71Combined sources4
Beta strandi74 – 76Combined sources3
Beta strandi79 – 82Combined sources4
Beta strandi85 – 91Combined sources7
Helixi93 – 96Combined sources4
Beta strandi99 – 101Combined sources3
Beta strandi106 – 112Combined sources7
Beta strandi116 – 121Combined sources6
Beta strandi125 – 127Combined sources3
Beta strandi134 – 141Combined sources8
Helixi150 – 153Combined sources4
Helixi177 – 179Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4C2MX-ray2.803/N1-233[»]
4C3HX-ray3.27N1-233[»]
4C3IX-ray3.0N1-233[»]
4C3JX-ray3.35N1-233[»]
4YM7X-ray5.50AN/BN/CN/DN/EN/FN1-233[»]
5G5Lelectron microscopy4.80N1-233[»]
ProteinModelPortaliP47006.
SMRiP47006.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi184 – 233Glu/Lys-richAdd BLAST50
Compositional biasi208 – 215Poly-Lys8
Compositional biasi221 – 230Poly-Lys10

Sequence similaritiesi

Phylogenomic databases

InParanoidiP47006.
KOiK03003.
OMAiHFPTGYG.
OrthoDBiEOG092C54OM.

Family and domain databases

InterProiIPR013240. DNA-dir_RNA_pol1_su_RPA34.
[Graphical view]
PfamiPF08208. RNA_polI_A34. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47006-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKLSKDYVS DSDSDDEVIS NEFSIPDGFK KCKHLKNFPL NGDNKKKAKQ
60 70 80 90 100
QQVWLIKFPS NVDISKLKSL PVDFESSTTM TIDKHDYKIM DDTDIESSLT
110 120 130 140 150
QDNLSNMTLL VPSESKESLK IASTAKDNAP LQFDKVFSVS ETAKIPAIDY
160 170 180 190 200
SKVRVPRKDV PKVEGLKLEH FATGYDAEDF HVAEEVKENK KEPKKRSHHD
210 220 230
DEEESSEKKK KKKEKREKRE KKDKKDKKKK HRD
Length:233
Mass (Da):26,875
Last modified:February 1, 1996 - v1
Checksum:iDCD42DFEC574EDB6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49423 Genomic DNA. Translation: CAA89443.1.
X87371 Genomic DNA. Translation: CAA60807.1.
BK006943 Genomic DNA. Translation: DAA08653.1.
PIRiS55165.
RefSeqiNP_012387.1. NM_001181581.1.

Genome annotation databases

EnsemblFungiiYJL148W; YJL148W; YJL148W.
GeneIDi853293.
KEGGisce:YJL148W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49423 Genomic DNA. Translation: CAA89443.1.
X87371 Genomic DNA. Translation: CAA60807.1.
BK006943 Genomic DNA. Translation: DAA08653.1.
PIRiS55165.
RefSeqiNP_012387.1. NM_001181581.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4C2MX-ray2.803/N1-233[»]
4C3HX-ray3.27N1-233[»]
4C3IX-ray3.0N1-233[»]
4C3JX-ray3.35N1-233[»]
4YM7X-ray5.50AN/BN/CN/DN/EN/FN1-233[»]
5G5Lelectron microscopy4.80N1-233[»]
ProteinModelPortaliP47006.
SMRiP47006.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33610. 327 interactors.
DIPiDIP-7973N.
IntActiP47006. 29 interactors.
MINTiMINT-2732801.

PTM databases

iPTMnetiP47006.

Proteomic databases

MaxQBiP47006.
PRIDEiP47006.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJL148W; YJL148W; YJL148W.
GeneIDi853293.
KEGGisce:YJL148W.

Organism-specific databases

EuPathDBiFungiDB:YJL148W.
SGDiS000003684. RPA34.

Phylogenomic databases

InParanoidiP47006.
KOiK03003.
OMAiHFPTGYG.
OrthoDBiEOG092C54OM.

Enzyme and pathway databases

BioCyciYEAST:G3O-31592-MONOMER.

Miscellaneous databases

PROiP47006.

Family and domain databases

InterProiIPR013240. DNA-dir_RNA_pol1_su_RPA34.
[Graphical view]
PfamiPF08208. RNA_polI_A34. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRPA34_YEAST
AccessioniPrimary (citable) accession number: P47006
Secondary accession number(s): D6VW37
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 2, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3360 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.