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P47006 (RPA34_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA-directed RNA polymerase I subunit RPA34

Short name=A34
Alternative name(s):
DNA-directed DNA-dependent RNA polymerase 34.5 kDa polypeptide
Short name=A34.5
Gene names
Name:RPA34
Ordered Locus Names:YJL148W
ORF Names:J0637
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length233 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

DNA-dependent RNA polymerases catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I (Pol I) which synthesizes ribosomal RNA precursors. Besides, RNA polymerase I has intrinsic RNA cleavage activity. The heterodimer formed by RPA34 and RPA49 stimulates transcript elongation by Pol I. Ref.4 Ref.9 Ref.15 Ref.16 Ref.17

Subunit structure

Component of the RNA polymerase I (Pol I) complex consisting of 14 subunits: RPA135, RPA190, RPC40, RPA14, RPB5, RPO26, RPA43, RPB8, RPA12, RPB10, RPC19, RPC10, RPA49 and RPA34. The complex is composed of a horseshoe-shaped core containing ten subunits (RPA135, RPA190, RPB5, RPO26, RPB8, RPB10, RPC10, RPA12, RPC19 and RPC40) where RPA135 and RPA190 form the DNA-binding cleft. Outside of the core, RPA14 and RPA43 form the stalk that mediates interactions with transcription initiation factors and newly synthesized RNA. Forms a TFIIF-like heterodimer with RPA49; the heterodimer formed by RPA34 and RPA49 can be dissociated from the Pol I core giving rise to a 12 subunit form A* of Pol I (formerly called pol A) that shows impaired transcript elongation activity and increased sensitivity to alpha-amanitin. The heterodimer formed by RPA34 and RPA49 stabilizes subunit RPA12 and stimulates RPA12-dependent RNA cleavage. Ref.4 Ref.5 Ref.9 Ref.11 Ref.12 Ref.15 Ref.16 Ref.17

Subcellular location

Nucleusnucleolus Ref.13.

Miscellaneous

Present with 3360 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the eukaryotic RPA34 RNA polymerase subunit family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 233233DNA-directed RNA polymerase I subunit RPA34
PRO_0000203031

Regions

Compositional bias184 – 23350Glu/Lys-rich
Compositional bias208 – 2158Poly-Lys
Compositional bias221 – 23010Poly-Lys

Amino acid modifications

Modified residue101Phosphoserine Ref.6 Ref.8
Modified residue121Phosphoserine Ref.6 Ref.8
Modified residue141Phosphoserine Ref.6 Ref.7 Ref.8
Modified residue601Phosphoserine Ref.7

Secondary structure

............................... 233
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P47006 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: DCD42DFEC574EDB6

FASTA23326,875
        10         20         30         40         50         60 
MSKLSKDYVS DSDSDDEVIS NEFSIPDGFK KCKHLKNFPL NGDNKKKAKQ QQVWLIKFPS 

        70         80         90        100        110        120 
NVDISKLKSL PVDFESSTTM TIDKHDYKIM DDTDIESSLT QDNLSNMTLL VPSESKESLK 

       130        140        150        160        170        180 
IASTAKDNAP LQFDKVFSVS ETAKIPAIDY SKVRVPRKDV PKVEGLKLEH FATGYDAEDF 

       190        200        210        220        230 
HVAEEVKENK KEPKKRSHHD DEEESSEKKK KKKEKREKRE KKDKKDKKKK HRD 

« Hide

References

« Hide 'large scale' references
[1]"Sequence analysis of a 40.7 kb segment from the left arm of yeast chromosome X reveals 14 known genes and 13 new open reading frames including homologues of genes clustered on the right arm of chromosome XI."
Katsoulou C., Tzermia M., Tavernarakis N., Alexandraki D.
Yeast 12:787-797(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[2]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"A34.5, a nonessential component of yeast RNA polymerase I, cooperates with subunit A14 and DNA topoisomerase I to produce a functional rRNA synthesis machine."
Gadal O., Mariotte-Labarre S., Chedin S., Quemeneur E., Carles C., Sentenac A., Thuriaux P.
Mol. Cell. Biol. 17:1787-1795(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, IDENTIFICATION IN THE RNA POL I COMPLEX, PHOSPHORYLATION.
[5]"Differential roles of phosphorylation in the formation of transcriptional active RNA polymerase I."
Fath S., Milkereit P., Peyroche G., Riva M., Carles C., Tschochner H.
Proc. Natl. Acad. Sci. U.S.A. 98:14334-14339(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE RNA POL I COMPLEX.
[6]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-10; SER-12 AND SER-14, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-14 AND SER-60, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-10; SER-12 AND SER-14, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"RNA polymerase I contains a TFIIF-related DNA-binding subcomplex."
Geiger S.R., Lorenzen K., Schreieck A., Hanecker P., Kostrewa D., Heck A.J., Cramer P.
Mol. Cell 39:583-594(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH RPA49, IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT.
[10]"Localization of the yeast RNA polymerase I-specific subunits."
Bischler N., Brino L., Carles C., Riva M., Tschochner H., Mallouh V., Schultz P.
EMBO J. 21:4136-4144(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: ELECTRON MICROSCOPY OF THE RNA POLYMERASE I COMPLEX.
[11]"Rpa12p, a conserved RNA polymerase I subunit with two functional domains."
Van Mullem V., Landrieux E., Vandenhaute J., Thuriaux P.
Mol. Microbiol. 43:1105-1113(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE RNA POL I COMPLEX.
[12]"The A14-A43 heterodimer subunit in yeast RNA pol I and their relationship to Rpb4-Rpb7 pol II subunits."
Peyroche G., Levillain E., Siaut M., Callebaut I., Schultz P., Sentenac A., Riva M., Carles C.
Proc. Natl. Acad. Sci. U.S.A. 99:14670-14675(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE RNA POL I COMPLEX.
[13]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[14]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[15]"Functional architecture of RNA polymerase I."
Kuhn C.D., Geiger S.R., Baumli S., Gartmann M., Gerber J., Jennebach S., Mielke T., Tschochner H., Beckmann R., Cramer P.
Cell 131:1260-1272(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY ELECTRON MICROSCOPY (12.00 ANGSTROMS) OF THE POL I COMPLEX, FUNCTION, SUBUNIT.
[16]"Crystal structure of the 14-subunit RNA polymerase I."
Fernandez-Tornero C., Moreno-Morcillo M., Rashid U.J., Taylor N.M., Ruiz F.M., Gruene T., Legrand P., Steuerwald U., Muller C.W.
Nature 502:644-649(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF THE POL I COMPLEX, FUNCTION, SUBUNIT.
[17]"RNA polymerase I structure and transcription regulation."
Engel C., Sainsbury S., Cheung A.C., Kostrewa D., Cramer P.
Nature 502:650-655(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF THE POL I COMPLEX, FUNCTION, SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z49423 Genomic DNA. Translation: CAA89443.1.
X87371 Genomic DNA. Translation: CAA60807.1.
BK006943 Genomic DNA. Translation: DAA08653.1.
PIRS55165.
RefSeqNP_012387.1. NM_001181581.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4C2MX-ray2.803/N1-233[»]
4C3HX-ray3.27N1-233[»]
4C3IX-ray3.0N1-233[»]
4C3JX-ray3.35N1-233[»]
ProteinModelPortalP47006.
SMRP47006. Positions 25-181.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33610. 322 interactions.
DIPDIP-7973N.
IntActP47006. 27 interactions.
MINTMINT-2732801.
STRING4932.YJL148W.

Proteomic databases

PaxDbP47006.
PeptideAtlasP47006.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJL148W; YJL148W; YJL148W.
GeneID853293.
KEGGsce:YJL148W.

Organism-specific databases

CYGDYJL148w.
SGDS000003684. RPA34.

Phylogenomic databases

eggNOGNOG39958.
KOK03003.
OMAHFPTGYG.
OrthoDBEOG7CK3K9.

Enzyme and pathway databases

BioCycYEAST:G3O-31592-MONOMER.

Gene expression databases

GenevestigatorP47006.

Family and domain databases

InterProIPR013240. DNA-dir_RNA_pol1_su_RPA34.
[Graphical view]
PfamPF08208. RNA_polI_A34. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio973600.

Entry information

Entry nameRPA34_YEAST
AccessionPrimary (citable) accession number: P47006
Secondary accession number(s): D6VW37
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: April 16, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references