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Protein

F-box protein DAS1

Gene

DAS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-31593-MONOMER.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
F-box protein DAS1
Alternative name(s):
DST1-delta 6-azauracil sensitivity protein 1
Gene namesi
Name:DAS1
Ordered Locus Names:YJL149W
ORF Names:J0634
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJL149W.
SGDiS000003685. DAS1.

Subcellular locationi

GO - Cellular componenti

  • SCF ubiquitin ligase complex Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 663663F-box protein DAS1PRO_0000119971Add
BLAST

Proteomic databases

MaxQBiP47005.
PRIDEiP47005.
TopDownProteomicsiP47005.

Interactioni

Subunit structurei

Interacts with SKP1. Component of the probable SCF(DAS1) complex containing CDC53, SKP1, RBX1 and DAS1.4 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi33609. 19 interactions.
DIPiDIP-6404N.
IntActiP47005. 4 interactions.
MINTiMINT-699003.

Structurei

3D structure databases

ProteinModelPortaliP47005.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini46 – 9146F-boxPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi533 – 5397Poly-Asp
Compositional biasi647 – 6526Poly-Asn

Sequence similaritiesi

Contains 1 F-box domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00530000066426.
InParanoidiP47005.
OMAiLTIISWN.
OrthoDBiEOG7TQV8F.

Family and domain databases

InterProiIPR001810. F-box_dom.
[Graphical view]
PfamiPF12937. F-box-like. 1 hit.
[Graphical view]
SMARTiSM00256. FBOX. 1 hit.
[Graphical view]
SUPFAMiSSF81383. SSF81383. 1 hit.
PROSITEiPS50181. FBOX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47005-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPFQDYFQKK KAAFINRNNK SNADASALRD INDININFAA KSKNYVFPLT
60 70 80 90 100
KLPDELMQEV FSHLPQPDRL QLCLVNKRLN KIATKLLYRR IYLNDSNVVK
110 120 130 140 150
SDFMHLAINW TLLNLPSSLK EEESRDIANC KLKKLIETLQ NNIHITEVIQ
160 170 180 190 200
WIRINWDLDS TLQRSILSIL CNQGKSLQRL ENVTDPACND IISNGHFSRS
210 220 230 240 250
NVSSFDMAPP NSLPEMVVPE NYIPNLTKYL SQRISSRLSH MTLFIDPLKL
260 270 280 290 300
FNYLYPLDIK LQIIDLKLHW RREFYNNDYF VKKIRPGNPL TKLSEVFDKR
310 320 330 340 350
TLKILTIISW NDTLLKRETE MLKDFKEFEN LEDLSLISIK QDVHILVDLF
360 370 380 390 400
SSLTNLKRLK MDFLEEYVPE PTNPHIFLSI LLACSKLQFI DLRYDGLIPQ
410 420 430 440 450
IINIQENKFQ LNQQCNCTNC QIVFSDILKG KIFMFPEDYY IHDLQDIAAK
460 470 480 490 500
DIFKMMKYLS LLPYSKACDA YPSVRTQPMN LTNFVTKMNR NLLEYRNSKS
510 520 530 540 550
QLVPKIVNNP HQHSTVTSTS TAHMSEPEMI IIDDDDDDDE INAAIPPSSD
560 570 580 590 600
DTAATISTDL ELPHESLTKR DIIMCYHALI HHFKSIYVTF LKSFPHLRFL
610 620 630 640 650
MLNDIPTIVM EENNERIFEP VFYHYDYKSN LYGWSKESNK NLENDSNNNN
660
NNSDTIARIA TVM
Length:663
Mass (Da):77,367
Last modified:February 1, 1996 - v1
Checksum:iC48B6CAB40920027
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49425 Genomic DNA. Translation: CAA89445.1.
X87371 Genomic DNA. Translation: CAA60806.1.
BK006943 Genomic DNA. Translation: DAA08652.1.
PIRiS55164.
RefSeqiNP_012386.1. NM_001181582.1.

Genome annotation databases

EnsemblFungiiYJL149W; YJL149W; YJL149W.
GeneIDi853292.
KEGGisce:YJL149W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49425 Genomic DNA. Translation: CAA89445.1.
X87371 Genomic DNA. Translation: CAA60806.1.
BK006943 Genomic DNA. Translation: DAA08652.1.
PIRiS55164.
RefSeqiNP_012386.1. NM_001181582.1.

3D structure databases

ProteinModelPortaliP47005.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33609. 19 interactions.
DIPiDIP-6404N.
IntActiP47005. 4 interactions.
MINTiMINT-699003.

Proteomic databases

MaxQBiP47005.
PRIDEiP47005.
TopDownProteomicsiP47005.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJL149W; YJL149W; YJL149W.
GeneIDi853292.
KEGGisce:YJL149W.

Organism-specific databases

EuPathDBiFungiDB:YJL149W.
SGDiS000003685. DAS1.

Phylogenomic databases

GeneTreeiENSGT00530000066426.
InParanoidiP47005.
OMAiLTIISWN.
OrthoDBiEOG7TQV8F.

Enzyme and pathway databases

UniPathwayiUPA00143.
BioCyciYEAST:G3O-31593-MONOMER.

Miscellaneous databases

PROiP47005.

Family and domain databases

InterProiIPR001810. F-box_dom.
[Graphical view]
PfamiPF12937. F-box-like. 1 hit.
[Graphical view]
SMARTiSM00256. FBOX. 1 hit.
[Graphical view]
SUPFAMiSSF81383. SSF81383. 1 hit.
PROSITEiPS50181. FBOX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of a 40.7 kb segment from the left arm of yeast chromosome X reveals 14 known genes and 13 new open reading frames including homologues of genes clustered on the right arm of chromosome XI."
    Katsoulou C., Tzermia M., Tavernarakis N., Alexandraki D.
    Yeast 12:787-797(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Cdc53/cullin and the essential Hrt1 RING-H2 subunit of SCF define a ubiquitin ligase module that activates the E2 enzyme Cdc34."
    Seol J.H., Feldman R.M.R., Zachariae W., Shevchenko A., Correll C.C., Lyapina S., Chi Y., Galova M., Claypool J., Sandmeyer S., Nasmyth K., Shevchenko A., Deshaies R.J.
    Genes Dev. 13:1614-1626(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN SCF COMPLEX.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN SCF COMPLEX.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Functional interaction of 13 yeast SCF complexes with a set of yeast E2 enzymes in vitro."
    Kus B.M., Caldon C.E., Andorn-Broza R., Edwards A.M.
    Proteins 54:455-467(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SKP1, RECONSTITUTION OF THE SCF(DAS1) COMPLEX.
  8. "Remodeling of the SCF complex-mediated ubiquitination system by compositional alteration of incorporated F-box proteins."
    Kato M., Kito K., Ota K., Ito T.
    Proteomics 10:115-123(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE SCF(DAS1) COMPLEX.

Entry informationi

Entry nameiDAS1_YEAST
AccessioniPrimary (citable) accession number: P47005
Secondary accession number(s): D6VW36
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: June 8, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 195 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.