P47005 (DAS1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
F-box protein DAS1

Alternative name(s):
DST1-delta 6-azauracil sensitivity protein 1

Gene names

Name:	DAS1
Ordered Locus Names:	YJL149W
ORF Names:	J0634

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	663 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins By similarity.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with SKP1. Component of the probable SCF(DAS1) complex containing CDC53, SKP1, RBX1 and DAS1. Ref.7
Miscellaneous	Present with 195 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Contains 1 F-box domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	SCF ubiquitin ligase complex Inferred from direct assay Ref.7. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 663	663	F-box protein DAS1		PRO_0000119971
Regions
	Domain	46 – 91	46	F-box
	Compositional bias	533 – 539	7	Poly-Asp
	Compositional bias	647 – 652	6	Poly-Asn

Sequences

	Sequence		Length	Mass (Da)
	P47005 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: C48B6CAB40920027	FASTA	663	77,367
	10 20 30 40 50 60 MPFQDYFQKK KAAFINRNNK SNADASALRD INDININFAA KSKNYVFPLT KLPDELMQEV 70 80 90 100 110 120 FSHLPQPDRL QLCLVNKRLN KIATKLLYRR IYLNDSNVVK SDFMHLAINW TLLNLPSSLK 130 140 150 160 170 180 EEESRDIANC KLKKLIETLQ NNIHITEVIQ WIRINWDLDS TLQRSILSIL CNQGKSLQRL 190 200 210 220 230 240 ENVTDPACND IISNGHFSRS NVSSFDMAPP NSLPEMVVPE NYIPNLTKYL SQRISSRLSH 250 260 270 280 290 300 MTLFIDPLKL FNYLYPLDIK LQIIDLKLHW RREFYNNDYF VKKIRPGNPL TKLSEVFDKR 310 320 330 340 350 360 TLKILTIISW NDTLLKRETE MLKDFKEFEN LEDLSLISIK QDVHILVDLF SSLTNLKRLK 370 380 390 400 410 420 MDFLEEYVPE PTNPHIFLSI LLACSKLQFI DLRYDGLIPQ IINIQENKFQ LNQQCNCTNC 430 440 450 460 470 480 QIVFSDILKG KIFMFPEDYY IHDLQDIAAK DIFKMMKYLS LLPYSKACDA YPSVRTQPMN 490 500 510 520 530 540 LTNFVTKMNR NLLEYRNSKS QLVPKIVNNP HQHSTVTSTS TAHMSEPEMI IIDDDDDDDE 550 560 570 580 590 600 INAAIPPSSD DTAATISTDL ELPHESLTKR DIIMCYHALI HHFKSIYVTF LKSFPHLRFL 610 620 630 640 650 660 MLNDIPTIVM EENNERIFEP VFYHYDYKSN LYGWSKESNK NLENDSNNNN NNSDTIARIA TVM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of a 40.7 kb segment from the left arm of yeast chromosome X reveals 14 known genes and 13 new open reading frames including homologues of genes clustered on the right arm of chromosome XI." Katsoulou C., Tzermia M., Tavernarakis N., Alexandraki D. Yeast 12:787-797(1996) [PubMed: 8813765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Cdc53/cullin and the essential Hrt1 RING-H2 subunit of SCF define a ubiquitin ligase module that activates the E2 enzyme Cdc34." Seol J.H., Feldman R.M.R., Zachariae W., Shevchenko A., Correll C.C., Lyapina S., Chi Y., Galova M., Claypool J., Sandmeyer S., Nasmyth K., Shevchenko A., Deshaies R.J. Genes Dev. 13:1614-1626(1999) [PubMed: 10385629] [Abstract] Cited for: IDENTIFICATION IN SCF COMPLEX.
[5]	"SCF ubiquitin protein ligases and phosphorylation-dependent proteolysis." Willems A.R., Goh T., Taylor L., Chernushevich I., Shevchenko A., Tyers M. Philos. Trans. R. Soc. Lond., B, Biol. Sci. 354:1533-1550(1999) [PubMed: 10582239] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN SCF COMPLEX.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Functional interaction of 13 yeast SCF complexes with a set of yeast E2 enzymes in vitro." Kus B.M., Caldon C.E., Andorn-Broza R., Edwards A.M. Proteins 54:455-467(2004) [PubMed: 14747994] [Abstract] Cited for: INTERACTION WITH SKP1, RECONSTITUTION OF THE SCF(DAS1) COMPLEX.
[8]	"Remodeling of the SCF complex-mediated ubiquitination system by compositional alteration of incorporated F-box proteins." Kato M., Kito K., Ota K., Ito T. Proteomics 10:115-123(2010) [PubMed: 19882662] [Abstract] Cited for: IDENTIFICATION IN THE SCF(DAS1) COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z49425 Genomic DNA. Translation: CAA89445.1. X87371 Genomic DNA. Translation: CAA60806.1. BK006943 Genomic DNA. Translation: DAA08652.1.
PIR	S55164.
RefSeq	NP_012386.1. NM_001181582.1.
3D structure databases
ProteinModelPortal	P47005.
SMR	P47005. Positions 47-94.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6404N.
IntAct	P47005. 3 interactions.
MINT	MINT-699003.
STRING	P47005.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJL149W; YJL149W; YJL149W.
GeneID	853292.
KEGG	sce:YJL149W.
NMPDR	fig|4932.3.peg.3351.
Organism-specific databases
CYGD	YJL149w.
SGD	S000003685. DAS1.
Phylogenomic databases
eggNOG	fuNOG04564.
GeneTree	EFGT00050000008602.
HOGENOM	HBG202288.
OMA	IYLNDSN.
OrthoDB	EOG415KNV.
Gene expression databases
ArrayExpress	P47005.
Genevestigator	P47005.
GermOnline	YJL149W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001810. F-box_dom_cyclin-like. IPR022364. F-box_dom_Skp2-like. [Graphical view]
Pfam	PF00646. F-box. 1 hit. [Graphical view]
SMART	SM00256. FBOX. 1 hit. [Graphical view]
SUPFAM	SSF81383. F-box_dom_Skp2-like. 1 hit.
PROSITE	PS50181. FBOX. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	973597.

Entry information

Entry name

DAS1_YEAST

Accession

Primary (citable) accession number: P47005
Secondary accession number(s): D6VW36

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	December 14, 2011
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program