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P47005 (DAS1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
F-box protein DAS1
Alternative name(s):
DST1-delta 6-azauracil sensitivity protein 1
Gene names
Name:DAS1
Ordered Locus Names:YJL149W
ORF Names:J0634
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length663 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins By similarity.

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Interacts with SKP1. Component of the probable SCF(DAS1) complex containing CDC53, SKP1, RBX1 and DAS1. Ref.7

Miscellaneous

Present with 195 molecules/cell in log phase SD medium. Ref.6

Sequence similarities

Contains 1 F-box domain.

Ontologies

Keywords
   Biological processUbl conjugation pathway
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular componentSCF ubiquitin ligase complex

Inferred from direct assay Ref.7. Source: SGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 663663F-box protein DAS1
PRO_0000119971

Regions

Domain46 – 9146F-box
Compositional bias533 – 5397Poly-Asp
Compositional bias647 – 6526Poly-Asn

Sequences

Sequence LengthMass (Da)Tools
P47005 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: C48B6CAB40920027

FASTA66377,367
        10         20         30         40         50         60 
MPFQDYFQKK KAAFINRNNK SNADASALRD INDININFAA KSKNYVFPLT KLPDELMQEV 

        70         80         90        100        110        120 
FSHLPQPDRL QLCLVNKRLN KIATKLLYRR IYLNDSNVVK SDFMHLAINW TLLNLPSSLK 

       130        140        150        160        170        180 
EEESRDIANC KLKKLIETLQ NNIHITEVIQ WIRINWDLDS TLQRSILSIL CNQGKSLQRL 

       190        200        210        220        230        240 
ENVTDPACND IISNGHFSRS NVSSFDMAPP NSLPEMVVPE NYIPNLTKYL SQRISSRLSH 

       250        260        270        280        290        300 
MTLFIDPLKL FNYLYPLDIK LQIIDLKLHW RREFYNNDYF VKKIRPGNPL TKLSEVFDKR 

       310        320        330        340        350        360 
TLKILTIISW NDTLLKRETE MLKDFKEFEN LEDLSLISIK QDVHILVDLF SSLTNLKRLK 

       370        380        390        400        410        420 
MDFLEEYVPE PTNPHIFLSI LLACSKLQFI DLRYDGLIPQ IINIQENKFQ LNQQCNCTNC 

       430        440        450        460        470        480 
QIVFSDILKG KIFMFPEDYY IHDLQDIAAK DIFKMMKYLS LLPYSKACDA YPSVRTQPMN 

       490        500        510        520        530        540 
LTNFVTKMNR NLLEYRNSKS QLVPKIVNNP HQHSTVTSTS TAHMSEPEMI IIDDDDDDDE 

       550        560        570        580        590        600 
INAAIPPSSD DTAATISTDL ELPHESLTKR DIIMCYHALI HHFKSIYVTF LKSFPHLRFL 

       610        620        630        640        650        660 
MLNDIPTIVM EENNERIFEP VFYHYDYKSN LYGWSKESNK NLENDSNNNN NNSDTIARIA 


TVM 

« Hide

References

« Hide 'large scale' references
[1]"Sequence analysis of a 40.7 kb segment from the left arm of yeast chromosome X reveals 14 known genes and 13 new open reading frames including homologues of genes clustered on the right arm of chromosome XI."
Katsoulou C., Tzermia M., Tavernarakis N., Alexandraki D.
Yeast 12:787-797(1996) [PubMed: 8813765] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[2]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Cdc53/cullin and the essential Hrt1 RING-H2 subunit of SCF define a ubiquitin ligase module that activates the E2 enzyme Cdc34."
Seol J.H., Feldman R.M.R., Zachariae W., Shevchenko A., Correll C.C., Lyapina S., Chi Y., Galova M., Claypool J., Sandmeyer S., Nasmyth K., Shevchenko A., Deshaies R.J.
Genes Dev. 13:1614-1626(1999) [PubMed: 10385629] [Abstract]
Cited for: IDENTIFICATION IN SCF COMPLEX.
[5]"SCF ubiquitin protein ligases and phosphorylation-dependent proteolysis."
Willems A.R., Goh T., Taylor L., Chernushevich I., Shevchenko A., Tyers M.
Philos. Trans. R. Soc. Lond., B, Biol. Sci. 354:1533-1550(1999) [PubMed: 10582239] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN SCF COMPLEX.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Functional interaction of 13 yeast SCF complexes with a set of yeast E2 enzymes in vitro."
Kus B.M., Caldon C.E., Andorn-Broza R., Edwards A.M.
Proteins 54:455-467(2004) [PubMed: 14747994] [Abstract]
Cited for: INTERACTION WITH SKP1, RECONSTITUTION OF THE SCF(DAS1) COMPLEX.
[8]"Remodeling of the SCF complex-mediated ubiquitination system by compositional alteration of incorporated F-box proteins."
Kato M., Kito K., Ota K., Ito T.
Proteomics 10:115-123(2010) [PubMed: 19882662] [Abstract]
Cited for: IDENTIFICATION IN THE SCF(DAS1) COMPLEX.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z49425 Genomic DNA. Translation: CAA89445.1.
X87371 Genomic DNA. Translation: CAA60806.1.
BK006943 Genomic DNA. Translation: DAA08652.1.
PIRS55164.
RefSeqNP_012386.1. NM_001181582.1.

3D structure databases

ProteinModelPortalP47005.
SMRP47005. Positions 47-94.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-6404N.
IntActP47005. 3 interactions.
MINTMINT-699003.
STRINGP47005.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJL149W; YJL149W; YJL149W.
GeneID853292.
KEGGsce:YJL149W.
NMPDRfig|4932.3.peg.3351.

Organism-specific databases

CYGDYJL149w.
SGDS000003685. DAS1.

Phylogenomic databases

eggNOGfuNOG04564.
GeneTreeEFGT00050000008602.
HOGENOMHBG202288.
OMAIYLNDSN.
OrthoDBEOG415KNV.

Gene expression databases

ArrayExpressP47005.
GenevestigatorP47005.
GermOnlineYJL149W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR001810. F-box_dom_cyclin-like.
IPR022364. F-box_dom_Skp2-like.
[Graphical view]
PfamPF00646. F-box. 1 hit.
[Graphical view]
SMARTSM00256. FBOX. 1 hit.
[Graphical view]
SUPFAMSSF81383. F-box_dom_Skp2-like. 1 hit.
PROSITEPS50181. FBOX. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio973597.

Entry information

Entry nameDAS1_YEAST
AccessionPrimary (citable) accession number: P47005
Secondary accession number(s): D6VW36
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: December 14, 2011
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families