Reviewed,
UniProtKB/Swiss-Prot P47002 (SSY5_YEAST)
Last modified
November 24, 2009.
Version 61.
History...
Clusters with 100%,
90%,
50% identity |
Documents (4) |
Third-party data |
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Names and origin
| Protein names | Recommended name: SPS-sensor serine protease component SSY5 Alternative name(s): Endoprotease SSY5 | ||||||||
| Gene names |
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| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||||||
| Taxonomic identifier | 4932 [NCBI] | ||||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 699 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Protease component of the SPS-sensor system, which regulates the expression of several amino acid-metabolizing enzymes and amino acid- and peptide-permeases in response to extracellular amino acid levels by controlling the activity of two transcription factors, STP1 and STP2. Catalyzes the activation of these transcription factors, which are synthesized as latent cytoplasmic precursors, by proteolytic removal of an N-terminal inhibitory domain containing cytoplasmic retention motifs. SSY5 binds as an inactive protease complex to STP1. In response to extracellular amino acids and dependent on the other SPS-sensor components, the inhibitory propeptide is induced to dissociate, and thereby enables the catalytic domain to process STP1. Ref.2 Ref.4 Ref.5 Ref.6 Ref.8 Ref.9 |
| Subunit structure | Component of the plasma membrane SPS (SSY1-PTR3-SSY5) amino acid sensor complex. |
| Subcellular location | Cell membrane; Peripheral membrane protein; Cytoplasmic side. |
| Induction | Down-regulated after extracellular amino-acid addition. |
| Post-translational modification | The propeptide is autoproteolytically cleaved from the catalytic domain but remains associated, forming an inactive protease complex. This processing occurs even in the absence of signaling. Ref.7 |
| Sequence similarities | Belongs to the peptidase S64 family. |
| Sequence caution | The sequence CAA89451.1 differs from that shown. Reason: Frameshift at position 685. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cell membrane Membrane |
| Molecular function | Hydrolase Protease |
| PTM | Zymogen |
| Technical term | Complete proteome Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | protein processing Ref.2 Ref.9 Inferred from direct assay. Source: SGD response to amino acid stimulus Ref.4Inferred from genetic interaction. Source: SGD |
| Cellular component | extrinsic to plasma membrane Ref.4 Inferred from direct assay. Source: SGD |
| Molecular function | serine-type endopeptidase activity Ref.2 Ref.9 Inferred from direct assay. Source: SGD |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Propeptide | 1 – 381 | 381 | PRO_0000377374 | ||||||
| Chain | 382 – 699 | 318 | SPS-sensor serine protease component SSY5 | PRO_0000072232 | |||||
Regions | |||||||||
| Region | 459 – 699 | 241 | Serine protease | ||||||
Sites | |||||||||
| Active site | 465 | 1 | Charge relay system Potential | ||||||
| Active site | 545 | 1 | Charge relay system Potential | ||||||
| Active site | 640 | 1 | Charge relay system Potential | ||||||
Experimental info | |||||||||
| Mutagenesis | 131 | 1 | E → K in SSY5-13; constitutively active, confers 9.3% increased STP1 processing in the absence of amino-acids. Ref.2 | ||||||
| Mutagenesis | 420 – 424 | 5 | Missing: Prevents maturation and amino-acid-induced STP1 cleavage. | ||||||
| Mutagenesis | 512 | 1 | E → K in SSY5-6; constitutively active, confers 30% increased STP1 processing in the absence of amino-acids. Ref.8 | ||||||
| Mutagenesis | 575 | 1 | F → V in SSY5-14; constitutively active, confers 30% increased STP1 processing in the absence of amino-acids. Ref.2 | ||||||
| Mutagenesis | 576 | 1 | Q → P in SSY5-15; constitutively active, confers 30% increased STP1 processing in the absence of amino-acids. Ref.2 | ||||||
| Mutagenesis | 581 | 1 | K → N in SSY5-18; constitutively active, confers 15.5% increased STP1 processing in the absence of amino-acids; when associated with H-632. Ref.2 | ||||||
| Mutagenesis | 632 | 1 | P → H in SSY5-18; constitutively active, confers 15.5% increased STP1 processing in the absence of amino-acids; when associated with N-581. Ref.2 | ||||||
| Mutagenesis | 640 | 1 | S → A: Impairs maturation and amino-acid-induced STP1 cleavage. Ref.7 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. Karpfinger-Hartl L.EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679. |
| [2] | "Mapping of an internal protease cleavage site in the Ssy5p component of the amino acid sensor of Saccharomyces cerevisiae and functional characterization of the resulting pro- and protease domains by gain-of-function genetics." Poulsen P., Lo Leggio L., Kielland-Brandt M.C. Eukaryot. Cell 5:601-608(2006) [PubMed: 16524914] [Abstract] Cited for: PROTEIN SEQUENCE OF 382-399, FUNCTION, MUTAGENESIS OF GLU-131; PHE-575; GLN-576; LYS-581 AND PRO-632. |
| [3] | "Mutations in five loci affecting GAP1-independent uptake of neutral amino acids in yeast." Joergensen M.U., Bruun M.B., Didion T., Kielland-Brandt M.C. Yeast 14:103-114(1998) [PubMed: 9483800] [Abstract] Cited for: IDENTIFICATION. |
| [4] | "Genetic and biochemical analysis of the yeast plasma membrane Ssy1p-Ptr3p-Ssy5p sensor of extracellular amino acids." Forsberg H., Ljungdahl P.O. Mol. Cell. Biol. 21:814-826(2001) [PubMed: 11154269] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION. |
| [5] | "Genetic analysis of the signalling pathway activated by external amino acids in Saccharomyces cerevisiae." Bernard F., Andre B. Mol. Microbiol. 41:489-502(2001) [PubMed: 11489133] [Abstract] Cited for: FUNCTION, INTERACTION WITH PTR3. |
| [6] | "Receptor-mediated endoproteolytic activation of two transcription factors in yeast." Andreasson C., Ljungdahl P.O. Genes Dev. 16:3158-3172(2002) [PubMed: 12502738] [Abstract] Cited for: FUNCTION. |
| [7] | "Amino acid signaling in yeast: casein kinase I and the Ssy5 endoprotease are key determinants of endoproteolytic activation of the membrane-bound Stp1 transcription factor." Abdel-Sater F., El Bakkoury M., Urrestarazu A., Vissers S., Andre B. Mol. Cell. Biol. 24:9771-9785(2004) [PubMed: 15509782] [Abstract] Cited for: AUTOPROTEOLYTIC MATURATION, CLEAVAGE OF STP1, MUTAGENESIS OF 420-PRO--SER-424 AND SER-640. |
| [8] | "Constitutive signal transduction by mutant Ssy5p and Ptr3p components of the SPS amino acid sensor system in Saccharomyces cerevisiae." Poulsen P., Wu B., Gaber R.F., Kielland-Brandt M.C. Eukaryot. Cell 4:1116-1124(2005) [PubMed: 15947203] [Abstract] Cited for: FUNCTION, IDENTIFICATION OF FRAMESHIFT, MUTAGENESIS OF GLU-512. |
| [9] | "Regulation of transcription factor latency by receptor-activated proteolysis." Andreasson C., Heessen S., Ljungdahl P.O. Genes Dev. 20:1563-1568(2006) [PubMed: 16778074] [Abstract] Cited for: FUNCTION, INTERACTION WITH STP1. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| Z49431 Genomic DNA. Translation: CAA89451.1. Frameshift. | |
| PIR | S56939. |
| RefSeq | NP_012379.1. |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP:6591N. |
| STRING | P47002. |
Protein family/group databases | |
| MEROPS | S64.001. |
Genome annotation databases | |
| Ensembl | YJL156C; YJL156C; YJL156C; Saccharomyces cerevisiae. [Genome view] |
| GeneID | 853285. |
| KEGG | sce:YJL156C. |
| NMPDR | fig|4932.3.peg.3344. |
Organism-specific databases | |
| CYGD | YJL156c. |
| SGD | S000003692. SSY5. |
Phylogenomic databases | |
| HOGENOM | P47002. |
| OrthoDB | EOG9SN34W |
Gene expression databases | |
| ArrayExpress | P47002. |
| Genevestigator | P47002. |
| GermOnline | YJL156C. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR009003. Ser/Cys_Pept_Trypsin-like. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 973582. |
Entry information
| Entry name | SSY5_YEAST | ||||||||
| Accession | Primary (citable) accession number: P47002 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| Peptidase families Classification of peptidase families and list of entries |
| SIMILARITY comments Index of protein domains and families |
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome X Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names |

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