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Protein

Cell wall mannoprotein CIS3

Gene

CIS3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the outer cell wall layer. Required for stability of the cell wall and for optimal growth. Required for resistance against several antifungal and cell wall-perturbing agents.2 Publications

Miscellaneous

Present with 12500 molecules/cell in log phase SD medium.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei74Covalent attachment to cell wall glycan1

GO - Molecular functioni

  • structural constituent of cell wall Source: SGD

GO - Biological processi

  • fungal-type cell wall organization Source: SGD

Keywordsi

Biological processCell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciYEAST:G3O-31598-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cell wall mannoprotein CIS3
Alternative name(s):
Covalently-linked cell wall protein 5/11
Protein with internal repeats 4
Soluble cell wall protein 8
Gene namesi
Name:CIS3
Synonyms:CCW11, CCW5, PIR4, SCW8
Ordered Locus Names:YJL158C
ORF Names:J0561
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJL158C.
SGDiS000003694. CIS3.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell wall, Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi65D → N: Does not affect cell wall incorporation. 1 Publication1
Mutagenesisi68S → A: Does not affect cell wall incorporation. 1 Publication1
Mutagenesisi69Q → A: Results in complete loss of cell wall incorporation. 1 Publication1
Mutagenesisi72D → N: Results in complete loss of cell wall incorporation. 1 Publication1
Mutagenesisi74Q → A: Results in complete loss of cell wall incorporation. 1 Publication1
Mutagenesisi76Q → A: Results in complete loss of cell wall incorporation. 1 Publication1
Mutagenesisi78T → A: Does not affect cell wall incorporation. 1 Publication1
Mutagenesisi79S → A: Does not affect cell wall incorporation. 1 Publication1
Mutagenesisi82T → A: Does not affect cell wall incorporation. 1 Publication1
Mutagenesisi130C → S: Does not affect cell wall incorporation. 1 Publication1
Mutagenesisi197C → S: Results in the incorporation of KEX2-unprocessed precursor protein into the cell wall. 1 Publication1
Mutagenesisi208 – 227QNVAE…SLVDC → LDC in PIR4t18; destabilizes the protein. 1 PublicationAdd BLAST20
Mutagenesisi227Missing : Does not affect cell wall incorporation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 211 PublicationAdd BLAST21
PropeptideiPRO_000003325822 – 646 PublicationsAdd BLAST43
ChainiPRO_000003325965 – 227Cell wall mannoprotein CIS3Add BLAST163

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi68O-linked (Man) serine1 Publication1
Glycosylationi78O-linked (Man) threonine1 Publication1
Glycosylationi105O-linked (Man) serine1 Publication1
Glycosylationi106O-linked (Man) serine1 Publication1
Glycosylationi107O-linked (Man) serine1 Publication1
Glycosylationi109O-linked (Man) serine1 Publication1
Glycosylationi111O-linked (Man) threonine1 Publication1
Glycosylationi112O-linked (Man) serine1 Publication1
Glycosylationi113O-linked (Man) threonine1 Publication1
Glycosylationi114N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi116O-linked (Man) threonine1 Publication1
Glycosylationi117O-linked (Man) serine1 Publication1
Glycosylationi118O-linked (Man) serine1 Publication1

Post-translational modificationi

Covalently linked to beta-1,3-glucan of the inner cell wall layer via an alkali-sensitive ester linkage between the gamma-carboxyl group of glutamic acid, arising from Gln-74 within the PIR1/2/3 repeat, and hydroxyl groups of glucoses of beta-1,3-glucan chains.
Extensively O-mannosylated. Also N-glycosylated.4 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei64 – 65Cleavage; by KEX22

Keywords - PTMi

Cleavage on pair of basic residues, Glycoprotein

Proteomic databases

MaxQBiP47001.
PRIDEiP47001.

PTM databases

iPTMnetiP47001.

Miscellaneous databases

PMAP-CutDBiP47001.

Expressioni

Inductioni

Positively regulated by signaling through MPK1 in response to cell wall perturbation.3 Publications

Interactioni

Protein-protein interaction databases

BioGridi33602. 83 interactors.
DIPiDIP-4788N.
ELMiP47001.
IntActiP47001. 3 interactors.
MINTiMINT-507442.
STRINGi4932.YJL158C.

Structurei

3D structure databases

ProteinModelPortaliP47001.
SMRiP47001.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati65 – 78PIR1/2/3Add BLAST14

Domaini

The PIR1/2/3 repeat is required for the covalent linkage to the cell wall.

Sequence similaritiesi

Belongs to the PIR protein family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

GeneTreeiENSGT00390000008137.
HOGENOMiHOG000248193.
InParanoidiP47001.
OMAiQFKNVAL.
OrthoDBiEOG092C3AY6.

Family and domain databases

InterProiView protein in InterPro
IPR000420. Yeast_PIR.
PfamiView protein in Pfam
PF00399. PIR. 1 hit.
PROSITEiView protein in PROSITE
PS00929. PIR_REPEAT_1. 1 hit.
PS50256. PIR_REPEAT_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P47001-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQFKNVALAA SVAALSATAS AEGYTPGEPW STLTPTGSIS CGAAEYTTTF
60 70 80 90 100
GIAVQAITSS KAKRDVISQI GDGQVQATSA ATAQATDSQA QATTTATPTS
110 120 130 140 150
SEKISSSASK TSTNATSSSC ATPSLKDSSC KNSGTLELTL KDGVLTDAKG
160 170 180 190 200
RIGSIVANRQ FQFDGPPPQA GAIYAAGWSI TEDGYLALGD SDVFYQCLSG
210 220
NFYNLYDQNV AEQCSAIHLE AVSLVDC
Length:227
Mass (Da):23,242
Last modified:February 1, 1996 - v1
Checksum:i9777D644CFF81880
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti43 – 44AA → SS AA sequence (PubMed:10438739).Curated2
Sequence conflicti68S → T AA sequence (PubMed:9301021).Curated1
Sequence conflicti88S → E AA sequence (PubMed:10438739).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49433 Genomic DNA. Translation: CAA89453.1.
AY693027 Genomic DNA. Translation: AAT93046.1.
BK006943 Genomic DNA. Translation: DAA08645.1.
PIRiS56941.
RefSeqiNP_012377.1. NM_001181591.1.

Genome annotation databases

EnsemblFungiiYJL158C; YJL158C; YJL158C.
GeneIDi853282.
KEGGisce:YJL158C.

Similar proteinsi

Entry informationi

Entry nameiCIS3_YEAST
AccessioniPrimary (citable) accession number: P47001
Secondary accession number(s): D6VW29
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: September 27, 2017
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names