ID   PIR5_YEAST              Reviewed;         287 AA.
AC   P46999; D6VW27; Q86ZS0;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   16-MAY-2006, sequence version 2.
DT   24-JAN-2024, entry version 130.
DE   RecName: Full=Cell wall protein PIR5;
DE   AltName: Full=Protein with internal repeats 5;
DE   Flags: Precursor;
GN   Name=PIR5; OrderedLocusNames=YJL160C; ORFNames=J0555;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=8641269; DOI=10.1002/j.1460-2075.1996.tb00557.x;
RA   Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C.,
RA   Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D.,
RA   Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J.,
RA   Heumann K., Hilger F., Hollenberg C.P., Huang M.-E., Jacq C.,
RA   Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E.,
RA   Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T.,
RA   Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B.,
RA   Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R.,
RA   Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N.,
RA   To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H.,
RA   von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.;
RT   "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.";
RL   EMBO J. 15:2031-2049(1996).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 144-223.
RC   STRAIN=ATCC 204511 / S288c / AB972;
RX   PubMed=12844361; DOI=10.1186/gb-2003-4-7-r45;
RA   Brachat S., Dietrich F.S., Voegeli S., Zhang Z., Stuart L., Lerch A.,
RA   Gates K., Gaffney T.D., Philippsen P.;
RT   "Reinvestigation of the Saccharomyces cerevisiae genome annotation by
RT   comparison to the genome of a related fungus: Ashbya gossypii.";
RL   Genome Biol. 4:R45.1-R45.13(2003).
RN   [4]
RP   FUNCTION.
RX   PubMed=12586695; DOI=10.1093/genetics/163.1.47;
RA   Enyenihi A.H., Saunders W.S.;
RT   "Large-scale functional genomic analysis of sporulation and meiosis in
RT   Saccharomyces cerevisiae.";
RL   Genetics 163:47-54(2003).
RN   [5]
RP   IDENTIFICATION OF FRAMESHIFT.
RX   PubMed=12748633; DOI=10.1038/nature01644;
RA   Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.;
RT   "Sequencing and comparison of yeast species to identify genes and
RT   regulatory elements.";
RL   Nature 423:241-254(2003).
RN   [6]
RP   INDUCTION.
RX   PubMed=16278933; DOI=10.1002/yea.1302;
RA   de Lichtenberg U., Wernersson R., Jensen T.S., Nielsen H.B., Fausboell A.,
RA   Schmidt P., Hansen F.B., Knudsen S., Brunak S.;
RT   "New weakly expressed cell cycle-regulated genes in yeast.";
RL   Yeast 22:1191-1201(2005).
CC   -!- FUNCTION: Component of the outer cell wall layer (By similarity). May
CC       be involved in meiosis and sporulation. {ECO:0000250,
CC       ECO:0000269|PubMed:12586695}.
CC   -!- SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000250}.
CC       Note=Covalently attached to the cell wall. {ECO:0000250}.
CC   -!- INDUCTION: Cell cycle-regulated. Expression peaks during mitosis.
CC       {ECO:0000269|PubMed:16278933}.
CC   -!- DOMAIN: The PIR1/2/3 repeats are required for the covalent linkage to
CC       the cell wall (By similarity). Their number varies among different
CC       strains of S.cerevisiae. {ECO:0000250}.
CC   -!- PTM: Covalently linked to beta-1,3-glucan of the inner cell wall layer
CC       via an alkali-sensitive ester linkage between the gamma-carboxyl group
CC       of glutamic acids, arising from specific glutamines within the PIR1/2/3
CC       repeats, and hydroxyl groups of glucoses of beta-1,3-glucan chains.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the PIR protein family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA89455.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; Z49435; CAA89455.1; ALT_FRAME; Genomic_DNA.
DR   EMBL; AY260893; AAP21761.1; -; Genomic_DNA.
DR   EMBL; BK006943; DAA08643.1; -; Genomic_DNA.
DR   PIR; S56943; S56943.
DR   RefSeq; NP_012375.2; NM_001181593.1.
DR   AlphaFoldDB; P46999; -.
DR   BioGRID; 33600; 311.
DR   DIP; DIP-1909N; -.
DR   IntAct; P46999; 2.
DR   MINT; P46999; -.
DR   STRING; 4932.YJL160C; -.
DR   PaxDb; 4932-YJL160C; -.
DR   PeptideAtlas; P46999; -.
DR   EnsemblFungi; YJL160C_mRNA; YJL160C; YJL160C.
DR   GeneID; 853280; -.
DR   KEGG; sce:YJL160C; -.
DR   AGR; SGD:S000003696; -.
DR   SGD; S000003696; PIR5.
DR   VEuPathDB; FungiDB:YJL160C; -.
DR   eggNOG; ENOG502QQD8; Eukaryota.
DR   GeneTree; ENSGT00940000176741; -.
DR   HOGENOM; CLU_039662_0_0_1; -.
DR   InParanoid; P46999; -.
DR   OMA; YEGTLEM; -.
DR   OrthoDB; 2106239at2759; -.
DR   BioCyc; YEAST:G3O-31600-MONOMER; -.
DR   BioGRID-ORCS; 853280; 2 hits in 10 CRISPR screens.
DR   PRO; PR:P46999; -.
DR   Proteomes; UP000002311; Chromosome X.
DR   RNAct; P46999; Protein.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR   GO; GO:0009277; C:fungal-type cell wall; IBA:GO_Central.
DR   GO; GO:0000324; C:fungal-type vacuole; HDA:SGD.
DR   GO; GO:0005199; F:structural constituent of cell wall; IBA:GO_Central.
DR   GO; GO:0031505; P:fungal-type cell wall organization; IBA:GO_Central.
DR   InterPro; IPR000420; Yeast_PIR.
DR   PANTHER; PTHR47254; CELL WALL MANNOPROTEIN CIS3-RELATED; 1.
DR   PANTHER; PTHR47254:SF1; CELL WALL MANNOPROTEIN CIS3-RELATED; 1.
DR   Pfam; PF00399; PIR; 3.
DR   PROSITE; PS00929; PIR_REPEAT_1; 4.
DR   PROSITE; PS50256; PIR_REPEAT_2; 4.
PE   2: Evidence at transcript level;
KW   Cell wall; Cleavage on pair of basic residues; Reference proteome; Repeat;
KW   Secreted; Signal.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   PROPEP          22..62
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000377623"
FT   CHAIN           63..287
FT                   /note="Cell wall protein PIR5"
FT                   /id="PRO_0000014333"
FT   REPEAT          62..80
FT                   /note="PIR1/2/3 1"
FT   REPEAT          81..99
FT                   /note="PIR1/2/3 2"
FT   REPEAT          104..122
FT                   /note="PIR1/2/3 3"
FT   REPEAT          144..162
FT                   /note="PIR1/2/3 4"
FT   SITE            62..63
FT                   /note="Cleavage; by KEX2"
FT                   /evidence="ECO:0000255"
FT   SITE            72
FT                   /note="Covalent attachment to cell wall glycan"
FT                   /evidence="ECO:0000250"
FT   SITE            91
FT                   /note="Covalent attachment to cell wall glycan"
FT                   /evidence="ECO:0000250"
FT   SITE            114
FT                   /note="Covalent attachment to cell wall glycan"
FT                   /evidence="ECO:0000250"
FT   SITE            154
FT                   /note="Covalent attachment to cell wall glycan"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   287 AA;  30215 MW;  3E02845EA0DD7F33 CRC64;
     MHYKKAFLAS LLSSIALTAY APPEPWATLT PSSKMDGGTT EYRTSFGLAV IPFTVTESKV
     KRNVISQIND GQVQVTTQKL PHPVSQIGDG QIQVTTQKVP PVVSHIVSQI GDGQLQITTA
     KNVVTKSTIA VPSKTVTATA TSTATAVSQI HDGQVQVTIS SASSSSVLSK SKLEPTKKPN
     NEKVIKVQAC KSSGTLAITL QGGVLIDSSG RIGSIVANRQ FQFDGPPPQA GAIYAGGWSI
     TKHGTLAIGD NDVFYQCLSG TFYNLYDQSI GGQCNPVHLQ TVGLVDC
//