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P46999 (PIR5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cell wall protein PIR5
Alternative name(s):
Protein with internal repeats 5
Gene names
Name:PIR5
Ordered Locus Names:YJL160C
ORF Names:J0555
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length287 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Component of the outer cell wall layer By similarity. May be involved in meiosis and sporulation. Ref.4

Subcellular location

Secretedcell wall By similarity. Note: Covalently attached to the cell wall By similarity.

Induction

Cell cycle-regulated. Expression peaks during mitosis. Ref.6

Domain

The PIR1/2/3 repeats are required for the covalent linkage to the cell wall By similarity. Their number varies among different strains of S.cerevisiae.

Post-translational modification

Covalently linked to beta-1,3-glucan of the inner cell wall layer via an alkali-sensitive ester linkage between the gamma-carboxyl group of glutamic acids, arising from specific glutamines within the PIR1/2/3 repeats, and hydroxyl groups of glucoses of beta-1,3-glucan chains By similarity.

Sequence similarities

Belongs to the PIR protein family.

Contains 4 PIR1/2/3 repeats.

Sequence caution

The sequence CAA89455.1 differs from that shown. Reason: Frameshift at position 174.

Ontologies

Keywords
   Cellular componentCell wall
Secreted
   DomainRepeat
Signal
   PTMCleavage on pair of basic residues
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcell wall

Inferred from electronic annotation. Source: UniProtKB-SubCell

extracellular region

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionstructural constituent of cell wall

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Propeptide22 – 6241 By similarity
PRO_0000377623
Chain63 – 287225Cell wall protein PIR5
PRO_0000014333

Regions

Repeat62 – 8019PIR1/2/3 1
Repeat81 – 9919PIR1/2/3 2
Repeat104 – 12219PIR1/2/3 3
Repeat144 – 16219PIR1/2/3 4

Sites

Site62 – 632Cleavage; by KEX2 Potential
Site721Covalent attachment to cell wall glycan By similarity
Site911Covalent attachment to cell wall glycan By similarity
Site1141Covalent attachment to cell wall glycan By similarity
Site1541Covalent attachment to cell wall glycan By similarity

Sequences

Sequence LengthMass (Da)Tools
P46999 [UniParc].

Last modified May 16, 2006. Version 2.
Checksum: 3E02845EA0DD7F33

FASTA28730,215
        10         20         30         40         50         60 
MHYKKAFLAS LLSSIALTAY APPEPWATLT PSSKMDGGTT EYRTSFGLAV IPFTVTESKV 

        70         80         90        100        110        120 
KRNVISQIND GQVQVTTQKL PHPVSQIGDG QIQVTTQKVP PVVSHIVSQI GDGQLQITTA 

       130        140        150        160        170        180 
KNVVTKSTIA VPSKTVTATA TSTATAVSQI HDGQVQVTIS SASSSSVLSK SKLEPTKKPN 

       190        200        210        220        230        240 
NEKVIKVQAC KSSGTLAITL QGGVLIDSSG RIGSIVANRQ FQFDGPPPQA GAIYAGGWSI 

       250        260        270        280 
TKHGTLAIGD NDVFYQCLSG TFYNLYDQSI GGQCNPVHLQ TVGLVDC 

« Hide

References

« Hide 'large scale' references
[1]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Reinvestigation of the Saccharomyces cerevisiae genome annotation by comparison to the genome of a related fungus: Ashbya gossypii."
Brachat S., Dietrich F.S., Voegeli S., Zhang Z., Stuart L., Lerch A., Gates K., Gaffney T.D., Philippsen P.
Genome Biol. 4:R45.1-R45.13(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 144-223.
Strain: ATCC 204511 / S288c / AB972.
[4]"Large-scale functional genomic analysis of sporulation and meiosis in Saccharomyces cerevisiae."
Enyenihi A.H., Saunders W.S.
Genetics 163:47-54(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"Sequencing and comparison of yeast species to identify genes and regulatory elements."
Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.
Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION OF FRAMESHIFT.
[6]"New weakly expressed cell cycle-regulated genes in yeast."
de Lichtenberg U., Wernersson R., Jensen T.S., Nielsen H.B., Fausboell A., Schmidt P., Hansen F.B., Knudsen S., Brunak S.
Yeast 22:1191-1201(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z49435 Genomic DNA. Translation: CAA89455.1. Frameshift.
AY260893 Genomic DNA. Translation: AAP21761.1.
BK006943 Genomic DNA. Translation: DAA08643.1.
PIRS56943.
RefSeqNP_012375.2. NM_001181593.1.

3D structure databases

ProteinModelPortalP46999.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33600. 106 interactions.
DIPDIP-1909N.
IntActP46999. 2 interactions.
MINTMINT-395257.
STRING4932.YJL160C.

Proteomic databases

PaxDbP46999.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJL160C; YJL160C; YJL160C.
GeneID853280.
KEGGsce:YJL160C.

Organism-specific databases

CYGDYJL160c.
SGDS000003696. YJL160C.

Phylogenomic databases

eggNOGNOG38801.
GeneTreeENSGT00390000008137.
HOGENOMHOG000248193.
OrthoDBEOG7FNCJZ.

Enzyme and pathway databases

BioCycYEAST:G3O-31600-MONOMER.

Gene expression databases

GenevestigatorP46999.

Family and domain databases

InterProIPR000420. Yeast_PIR.
[Graphical view]
PfamPF00399. PIR. 4 hits.
[Graphical view]
PROSITEPS00929. PIR_REPEAT_1. 4 hits.
PS50256. PIR_REPEAT_2. 4 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio973570.
PMAP-CutDBP46999.

Entry information

Entry namePIR5_YEAST
AccessionPrimary (citable) accession number: P46999
Secondary accession number(s): D6VW27, Q86ZS0
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: May 16, 2006
Last modified: July 9, 2014
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families