P46999 (PIR5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cell wall protein PIR5

Alternative name(s):
Protein with internal repeats 5

Gene names

Name:	PIR5
Ordered Locus Names:	YJL160C
ORF Names:	J0555

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	287 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Component of the outer cell wall layer By similarity. May be involved in meiosis and sporulation. Ref.4
Subcellular location	Secreted › cell wall By similarity. Note: Covalently attached to the cell wall By similarity.
Induction	Cell cycle-regulated. Expression peaks during mitosis. Ref.6
Domain	The PIR1/2/3 repeats are required for the covalent linkage to the cell wall By similarity. Their number varies among different strains of S.cerevisiae.
Post-translational modification	Covalently linked to beta-1,3-glucan of the inner cell wall layer via an alkali-sensitive ester linkage between the gamma-carboxyl group of glutamic acids, arising from specific glutamines within the PIR1/2/3 repeats, and hydroxyl groups of glucoses of beta-1,3-glucan chains By similarity.
Sequence similarities	Belongs to the PIR protein family. Contains 4 PIR1/2/3 repeats.
Sequence caution	The sequence CAA89455.1 differs from that shown. Reason: Frameshift at position 174.

Ontologies

Keywords
Cellular component	Cell wall Secreted
Domain	Repeat Signal
PTM	Cleavage on pair of basic residues
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	structural constituent of cell wall Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Propeptide	22 – 62	41	By similarity		PRO_0000377623
	Chain	63 – 287	225	Cell wall protein PIR5		PRO_0000014333
Regions
	Repeat	62 – 80	19	PIR1/2/3 1
	Repeat	81 – 99	19	PIR1/2/3 2
	Repeat	104 – 122	19	PIR1/2/3 3
	Repeat	144 – 162	19	PIR1/2/3 4
Sites
	Site	62 – 63	2	Cleavage; by KEX2 Potential
	Site	72	1	Covalent attachment to cell wall glycan By similarity
	Site	91	1	Covalent attachment to cell wall glycan By similarity
	Site	114	1	Covalent attachment to cell wall glycan By similarity
	Site	154	1	Covalent attachment to cell wall glycan By similarity

Sequences

	Sequence		Length	Mass (Da)
	P46999 [UniParc]. Last modified May 16, 2006. Version 2. Checksum: 3E02845EA0DD7F33	FASTA	287	30,215
	10 20 30 40 50 60 MHYKKAFLAS LLSSIALTAY APPEPWATLT PSSKMDGGTT EYRTSFGLAV IPFTVTESKV 70 80 90 100 110 120 KRNVISQIND GQVQVTTQKL PHPVSQIGDG QIQVTTQKVP PVVSHIVSQI GDGQLQITTA 130 140 150 160 170 180 KNVVTKSTIA VPSKTVTATA TSTATAVSQI HDGQVQVTIS SASSSSVLSK SKLEPTKKPN 190 200 210 220 230 240 NEKVIKVQAC KSSGTLAITL QGGVLIDSSG RIGSIVANRQ FQFDGPPPQA GAIYAGGWSI 250 260 270 280 TKHGTLAIGD NDVFYQCLSG TFYNLYDQSI GGQCNPVHLQ TVGLVDC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Reinvestigation of the Saccharomyces cerevisiae genome annotation by comparison to the genome of a related fungus: Ashbya gossypii." Brachat S., Dietrich F.S., Voegeli S., Zhang Z., Stuart L., Lerch A., Gates K., Gaffney T.D., Philippsen P. Genome Biol. 4:R45.1-R45.13(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 144-223. Strain: ATCC 204511 / S288c / AB972.
[4]	"Large-scale functional genomic analysis of sporulation and meiosis in Saccharomyces cerevisiae." Enyenihi A.H., Saunders W.S. Genetics 163:47-54(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"Sequencing and comparison of yeast species to identify genes and regulatory elements." Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S. Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION OF FRAMESHIFT.
[6]	"New weakly expressed cell cycle-regulated genes in yeast." de Lichtenberg U., Wernersson R., Jensen T.S., Nielsen H.B., Fausboell A., Schmidt P., Hansen F.B., Knudsen S., Brunak S. Yeast 22:1191-1201(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z49435 Genomic DNA. Translation: CAA89455.1. Frameshift. AY260893 Genomic DNA. Translation: AAP21761.1. BK006943 Genomic DNA. Translation: DAA08643.1.
PIR	S56943.
RefSeq	NP_012375.2. NM_001181593.1.
3D structure databases
ProteinModelPortal	P46999.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1909N.
IntAct	P46999. 2 interactions.
MINT	MINT-395257.
STRING	4932.YJL160C.
Proteomic databases
PaxDb	P46999.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJL160C; YJL160C; YJL160C.
GeneID	853280.
KEGG	sce:YJL160C.
Organism-specific databases
CYGD	YJL160c.
SGD	S000003696. YJL160C.
Phylogenomic databases
eggNOG	NOG38801.
GeneTree	ENSGT00390000008137.
HOGENOM	HOG000248193.
OrthoDB	EOG4GMZ6K.
Gene expression databases
Genevestigator	P46999.
GermOnline	YJL160C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000420. Yeast_PIR. [Graphical view]
Pfam	PF00399. PIR. 4 hits. [Graphical view]
PROSITE	PS00929. PIR_REPEAT_1. 4 hits. PS50256. PIR_REPEAT_2. 4 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	973570.
PMAP-CutDB	P46999.

Entry information

Entry name

PIR5_YEAST

Accession

Primary (citable) accession number: P46999
Secondary accession number(s): D6VW27, Q86ZS0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	May 16, 2006
Last modified:	April 3, 2013
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program