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P46999

- PIR5_YEAST

UniProt

P46999 - PIR5_YEAST

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Protein
Cell wall protein PIR5
Gene
PIR5, YJL160C, J0555
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Component of the outer cell wall layer By similarity. May be involved in meiosis and sporulation.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei62 – 632Cleavage; by KEX2 Reviewed prediction
Sitei72 – 721Covalent attachment to cell wall glycan By similarity
Sitei91 – 911Covalent attachment to cell wall glycan By similarity
Sitei114 – 1141Covalent attachment to cell wall glycan By similarity
Sitei154 – 1541Covalent attachment to cell wall glycan By similarity

GO - Molecular functioni

  1. structural constituent of cell wall Source: InterPro
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-31600-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cell wall protein PIR5
Alternative name(s):
Protein with internal repeats 5
Gene namesi
Name:PIR5
Ordered Locus Names:YJL160C
ORF Names:J0555
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

CYGDiYJL160c.
SGDiS000003696. YJL160C.

Subcellular locationi

Secretedcell wall By similarity
Note: Covalently attached to the cell wall By similarity.

GO - Cellular componenti

  1. cell wall Source: UniProtKB-SubCell
  2. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121 Reviewed prediction
Add
BLAST
Propeptidei22 – 6241 By similarity
PRO_0000377623Add
BLAST
Chaini63 – 287225Cell wall protein PIR5
PRO_0000014333Add
BLAST

Post-translational modificationi

Covalently linked to beta-1,3-glucan of the inner cell wall layer via an alkali-sensitive ester linkage between the gamma-carboxyl group of glutamic acids, arising from specific glutamines within the PIR1/2/3 repeats, and hydroxyl groups of glucoses of beta-1,3-glucan chains By similarity.

Keywords - PTMi

Cleavage on pair of basic residues

Proteomic databases

PaxDbiP46999.

Miscellaneous databases

PMAP-CutDBP46999.

Expressioni

Inductioni

Cell cycle-regulated. Expression peaks during mitosis.1 Publication

Gene expression databases

GenevestigatoriP46999.

Interactioni

Protein-protein interaction databases

BioGridi33600. 106 interactions.
DIPiDIP-1909N.
IntActiP46999. 2 interactions.
MINTiMINT-395257.
STRINGi4932.YJL160C.

Structurei

3D structure databases

ProteinModelPortaliP46999.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati62 – 8019PIR1/2/3 1
Add
BLAST
Repeati81 – 9919PIR1/2/3 2
Add
BLAST
Repeati104 – 12219PIR1/2/3 3
Add
BLAST
Repeati144 – 16219PIR1/2/3 4
Add
BLAST

Domaini

The PIR1/2/3 repeats are required for the covalent linkage to the cell wall By similarity. Their number varies among different strains of S.cerevisiae.

Sequence similaritiesi

Belongs to the PIR protein family.
Contains 4 PIR1/2/3 repeats.

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG38801.
GeneTreeiENSGT00390000008137.
HOGENOMiHOG000248193.
OrthoDBiEOG7FNCJZ.

Family and domain databases

InterProiIPR000420. Yeast_PIR.
[Graphical view]
PfamiPF00399. PIR. 4 hits.
[Graphical view]
PROSITEiPS00929. PIR_REPEAT_1. 4 hits.
PS50256. PIR_REPEAT_2. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P46999-1 [UniParc]FASTAAdd to Basket

« Hide

MHYKKAFLAS LLSSIALTAY APPEPWATLT PSSKMDGGTT EYRTSFGLAV    50
IPFTVTESKV KRNVISQIND GQVQVTTQKL PHPVSQIGDG QIQVTTQKVP 100
PVVSHIVSQI GDGQLQITTA KNVVTKSTIA VPSKTVTATA TSTATAVSQI 150
HDGQVQVTIS SASSSSVLSK SKLEPTKKPN NEKVIKVQAC KSSGTLAITL 200
QGGVLIDSSG RIGSIVANRQ FQFDGPPPQA GAIYAGGWSI TKHGTLAIGD 250
NDVFYQCLSG TFYNLYDQSI GGQCNPVHLQ TVGLVDC 287
Length:287
Mass (Da):30,215
Last modified:May 16, 2006 - v2
Checksum:i3E02845EA0DD7F33
GO

Sequence cautioni

The sequence CAA89455.1 differs from that shown. Reason: Frameshift at position 174.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z49435 Genomic DNA. Translation: CAA89455.1. Frameshift.
AY260893 Genomic DNA. Translation: AAP21761.1.
BK006943 Genomic DNA. Translation: DAA08643.1.
PIRiS56943.
RefSeqiNP_012375.2. NM_001181593.1.

Genome annotation databases

EnsemblFungiiYJL160C; YJL160C; YJL160C.
GeneIDi853280.
KEGGisce:YJL160C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z49435 Genomic DNA. Translation: CAA89455.1 . Frameshift.
AY260893 Genomic DNA. Translation: AAP21761.1 .
BK006943 Genomic DNA. Translation: DAA08643.1 .
PIRi S56943.
RefSeqi NP_012375.2. NM_001181593.1.

3D structure databases

ProteinModelPortali P46999.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33600. 106 interactions.
DIPi DIP-1909N.
IntActi P46999. 2 interactions.
MINTi MINT-395257.
STRINGi 4932.YJL160C.

Proteomic databases

PaxDbi P46999.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YJL160C ; YJL160C ; YJL160C .
GeneIDi 853280.
KEGGi sce:YJL160C.

Organism-specific databases

CYGDi YJL160c.
SGDi S000003696. YJL160C.

Phylogenomic databases

eggNOGi NOG38801.
GeneTreei ENSGT00390000008137.
HOGENOMi HOG000248193.
OrthoDBi EOG7FNCJZ.

Enzyme and pathway databases

BioCyci YEAST:G3O-31600-MONOMER.

Miscellaneous databases

NextBioi 973570.
PMAP-CutDB P46999.

Gene expression databases

Genevestigatori P46999.

Family and domain databases

InterProi IPR000420. Yeast_PIR.
[Graphical view ]
Pfami PF00399. PIR. 4 hits.
[Graphical view ]
PROSITEi PS00929. PIR_REPEAT_1. 4 hits.
PS50256. PIR_REPEAT_2. 4 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Reinvestigation of the Saccharomyces cerevisiae genome annotation by comparison to the genome of a related fungus: Ashbya gossypii."
    Brachat S., Dietrich F.S., Voegeli S., Zhang Z., Stuart L., Lerch A., Gates K., Gaffney T.D., Philippsen P.
    Genome Biol. 4:R45.1-R45.13(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 144-223.
    Strain: ATCC 204511 / S288c / AB972.
  4. "Large-scale functional genomic analysis of sporulation and meiosis in Saccharomyces cerevisiae."
    Enyenihi A.H., Saunders W.S.
    Genetics 163:47-54(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Sequencing and comparison of yeast species to identify genes and regulatory elements."
    Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.
    Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF FRAMESHIFT.
  6. Cited for: INDUCTION.

Entry informationi

Entry nameiPIR5_YEAST
AccessioniPrimary (citable) accession number: P46999
Secondary accession number(s): D6VW27, Q86ZS0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: May 16, 2006
Last modified: July 9, 2014
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3

Similar proteinsi