Reviewed, UniProtKB/Swiss-Prot P46997 (JJJ2_YEAST)

Names and origin

Protein names

Recommended name:
    J protein JJJ2

Gene names

Name:	JJJ2
Ordered Locus Names:	YJL162C
ORF Names:	J0549

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	583 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subcellular location	Cytoplasm. Nucleus.
Miscellaneous	Present with 184 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Contains 1 J domain.
Sequence caution	The sequence CAA89457.1 differs from that shown. Reason: Erroneous termination at position 483. Translated as Leu.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Molecular function	Chaperone
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein folding Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Ref.4 Inferred from direct assay. Source: SGD nucleus Ref.4 Inferred from direct assay. Source: SGD
Molecular function	heat shock protein binding Inferred from electronic annotation. Source: InterPro unfolded protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 583	583	J protein JJJ2		PRO_0000071142
Regions
	Domain	11 – 79	69	J
	Compositional bias	232 – 236	5	Poly-Glu
	Compositional bias	240 – 252	13	Poly-Gln
Amino acid modifications
	Modified residue	105	1	Phosphoserine Ref.7
	Modified residue	229	1	Phosphoserine Ref.8

Sequences

	Sequence		Length	Mass (Da)
	P46997-1 [UniParc]. Last modified May 20, 2008. Version 2. Checksum: 6CBB8ED37F5F340B	FASTA	583	67,389
	10 20 30 40 50 60 MSQVIEPQLD RTTYYSILGL TSNATSSEVH KSYLKLARLL HPDKTKSDKS EELFKAVVHA 70 80 90 100 110 120 HSILTDEDQK LRYDRDLKIK GLHTYQPKKN CHIFKTKAKE SQGASPTLGQ SEAYHRQNKP 130 140 150 160 170 180 YEQQPYGFGV GKKMTSSSKS KVPIFKSFNL KSYQRNHYYS SKKERKHGSP DIDSLFHETN 190 200 210 220 230 240 GASKVRMTDA GKMDTNSQFQ EIWEILGKNA YTHKSYSEDP NSCLGSALSD HEEEEEAGKQ 250 260 270 280 290 300 QQQQQQQQQQ QQHYGMTSKS SSPDEEKKNN KEPKRESRVS PEENGEEETG HKQFKLPKTS 310 320 330 340 350 360 TFSSGSHDSN LQSPFYNHEY RHYARSKFEC KNQFRKSVSP IKEIPATTSA NEGWNILRDI 370 380 390 400 410 420 IEKLNISNVD DRNKDLLFRR DEIGDKNHSD SIDIENLSIK EPKGMKRRKK DDISLEELFQ 430 440 450 460 470 480 SLPREKDYFM MDAINDSLES INLFKKPKTT QSHEQGGTFA QAESNRAKFK PLLEQCGITP 490 500 510 520 530 540 EILDLEIPEI PEFDAVADLE TLKLNVQLFN NQCNKLKETI HQVSLQRLRA DTQFSDMLTQ 550 560 570 580 KQSIMVWKTY LEFDKSLMDK LNILQERQMQ VIKIFSERCD GKV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database (SGD) Unpublished observations (JAN-2006) Cited for: SEQUENCE REVISION TO 433 AND 483.
[3]	"Sequencing and comparison of yeast species to identify genes and regulatory elements." Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S. Nature 423:241-254(2003) [PubMed: 12748633] [Abstract] Cited for: IDENTIFICATION OF PROBABLE SEQUENCE ERRORS.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"The J-protein family: modulating protein assembly, disassembly and translocation." Walsh P., Bursac D., Law Y.C., Cyr D., Lithgow T. EMBO Rep. 5:567-571(2004) [PubMed: 15170475] [Abstract] Cited for: GENE NAME.
[7]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-105, MASS SPECTROMETRY.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-229, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z49437 Genomic DNA. Translation: CAA89457.1. Sequence problems.
PIR	S56945.
RefSeq	NP_012373.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4033N.
IntAct	P46997. 2 interactions.
STRING	P46997.
Genome annotation databases
Ensembl	YJL162C; YJL162C; YJL162C; Saccharomyces cerevisiae. [Genome view]
GeneID	853277.
KEGG	sce:YJL162C.
NMPDR	fig|4932.3.peg.3337.
Organism-specific databases
CYGD	YJL162c.
SGD	S000003698. JJJ2.
Phylogenomic databases
OrthoDB	EOG9HQG25
Gene expression databases
ArrayExpress	P46997.
Genevestigator	P46997.
GermOnline	YJL162C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001623. DnaJ_N. IPR018253. Heat_shock_DnaJ_CS. IPR015609. Hsp40/DnaJ_Rel. IPR003095. Hsp_DnaJ. [Graphical view]
Gene3D	G3DSA:1.10.287.110. DnaJ_N. 1 hit.
PANTHER	PTHR11821. Hsp40/DnaJ_Rel. 1 hit.
Pfam	PF00226. DnaJ. 1 hit. [Graphical view]
PRINTS	PR00625. DNAJPROTEIN.
SMART	SM00271. DnaJ. 1 hit. [Graphical view]
PROSITE	PS00636. DNAJ_1. 1 hit. PS50076. DNAJ_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	973562.

Entry information

Entry name

JJJ2_YEAST

Accession

Primary (citable) accession number: P46997

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	May 20, 2008
Last modified:	November 24, 2009
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)