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P46995

- SET2_YEAST

UniProt

P46995 - SET2_YEAST

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Protein
Histone-lysine N-methyltransferase, H3 lysine-36 specific
Gene
SET2, EZL1, KMT3, YJL168C, J0520
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Histone methyltransferase that methylates histone H3 to form H3K36me. Involved in transcription elongation as well as in transcription repression. The methyltransferase activity requires the recruitment to the RNA polymerase II, which is CTK1 dependent.7 Publications

Catalytic activityi

S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].

GO - Molecular functioni

  1. histone methyltransferase activity Source: SGD
  2. histone methyltransferase activity (H3-K36 specific) Source: SGD
Complete GO annotation...

GO - Biological processi

  1. DNA-templated transcription, elongation Source: SGD
  2. DNA-templated transcription, termination Source: SGD
  3. ascospore formation Source: SGD
  4. histone deacetylation Source: SGD
  5. histone methylation Source: SGD
  6. negative regulation of antisense RNA transcription Source: SGD
  7. negative regulation of histone H3-K14 acetylation Source: SGD
  8. negative regulation of histone H3-K9 acetylation Source: SGD
  9. negative regulation of reciprocal meiotic recombination Source: SGD
  10. positive regulation of histone acetylation Source: SGD
  11. regulation of DNA-dependent DNA replication initiation Source: SGD
  12. regulation of histone exchange Source: SGD
  13. regulation of transcription, DNA-templated Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Repressor, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciYEAST:G3O-31606-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Histone-lysine N-methyltransferase, H3 lysine-36 specific (EC:2.1.1.43)
Alternative name(s):
Lysine N-methyltransferase 3
SET domain-containing protein 2
Gene namesi
Name:SET2
Synonyms:EZL1, KMT3
Ordered Locus Names:YJL168C
ORF Names:J0520
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

CYGDiYJL168c.
SGDiS000003704. SET2.

Subcellular locationi

GO - Cellular componenti

  1. chromosome Source: UniProtKB-SubCell
  2. cytosol Source: SGD
  3. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi195 – 1951R → G: Reduces dramatically histone methyltransferase activity toward nucleosomes. 1 Publication
Mutagenesisi201 – 2011C → A: Reduces dramatically histone methyltransferase activity toward nucleosomes. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 733733Histone-lysine N-methyltransferase, H3 lysine-36 specific
PRO_0000186087Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei10 – 101Phosphoserine1 Publication
Modified residuei522 – 5221Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP46995.
PaxDbiP46995.
PeptideAtlasiP46995.

Expressioni

Gene expression databases

GenevestigatoriP46995.

Interactioni

Subunit structurei

Interacts with the RNA polymerase II hyperphosphorylated CTD. Interacts with CYC8.7 Publications

Protein-protein interaction databases

BioGridi33591. 392 interactions.
DIPiDIP-2150N.
IntActiP46995. 62 interactions.
MINTiMINT-500810.
STRINGi4932.YJL168C.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi481 – 49414
Turni495 – 4984
Beta strandi499 – 5035
Helixi624 – 64522
Turni648 – 6525
Helixi655 – 67622
Helixi688 – 71225

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1E0NNMR-A479-505[»]
2C5ZNMR-A620-719[»]
ProteinModelPortaliP46995.
SMRiP46995. Positions 66-261, 479-505, 622-703.

Miscellaneous databases

EvolutionaryTraceiP46995.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini63 – 11856AWS
Add
BLAST
Domaini120 – 237118SET
Add
BLAST
Domaini244 – 26017Post-SET
Add
BLAST
Domaini475 – 50733WW
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni619 – 718100Binding to RNA polymerase II CTD
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili548 – 63083 Reviewed prediction
Add
BLAST

Domaini

The AWS and SET domains are necessary for transcription repression.2 Publications

Sequence similaritiesi

Contains 1 AWS domain.
Contains 1 post-SET domain.
Contains 1 SET domain.
Contains 1 WW domain.

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG2940.
GeneTreeiENSGT00750000117355.
HOGENOMiHOG000248214.
KOiK11423.
OMAiSCNPNAY.
OrthoDBiEOG7P8PH3.

Family and domain databases

InterProiIPR006560. AWS.
IPR025788. Hist-Lys_N-MeTrfase_SET2_fun.
IPR003616. Post-SET_dom.
IPR001214. SET_dom.
IPR013257. SRI.
IPR001202. WW_dom.
[Graphical view]
PfamiPF00856. SET. 1 hit.
PF08236. SRI. 1 hit.
[Graphical view]
SMARTiSM00570. AWS. 1 hit.
SM00508. PostSET. 1 hit.
SM00317. SET. 1 hit.
SM00456. WW. 1 hit.
[Graphical view]
SUPFAMiSSF51045. SSF51045. 1 hit.
PROSITEiPS51215. AWS. 1 hit.
PS50868. POST_SET. 1 hit.
PS51568. SAM_MT43_SET2_1. 1 hit.
PS50280. SET. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P46995-1 [UniParc]FASTAAdd to Basket

« Hide

MSKNQSVSAS EDEKEILNNN AEGHKPQRLF DQEPDLTEEA LTKFENLDDC    50
IYANKRIGTF KNNDFMECDC YEEFSDGVNH ACDEDSDCIN RLTLIECVND 100
LCSSCGNDCQ NQRFQKKQYA PIAIFKTKHK GYGVRAEQDI EANQFIYEYK 150
GEVIEEMEFR DRLIDYDQRH FKHFYFMMLQ NGEFIDATIK GSLARFCNHS 200
CSPNAYVNKW VVKDKLRMGI FAQRKILKGE EITFDYNVDR YGAQAQKCYC 250
EEPNCIGFLG GKTQTDAASL LPQNIADALG VTVSMEKKWL KLKKLSGEPI 300
IKNENENINI EFLQSLEVQP IDSPVDVTKI MSVLLQQDNK IIASKLLKRL 350
FTIDDDSLRH QAIKLHGYTC FSKMLKLFIT EQPQVDGKGN ETEEDDIKFI 400
KGILDFLLEL PKTTRNGIES SQIDNVVKTL PAKFPFLKPN CDELLEKWSK 450
FETYKRITKK DINVAASKMI DLRRVRLPPG WEIIHENGRP LYYNAEQKTK 500
LHYPPSGSSK VFSSRSNTQV NSPSSSGIPK TPGALDSKKH KLSDEEYERK 550
KQKRLEYERI ALERAKQEEL ESLKQKLKLE NERKSVLEDI IAEANKQKEL 600
QKEEAKKLVE AKEAKRLKRK TVSQSQRLEH NWNKFFASFV PNLIKKNPQS 650
KQFDHENIKQ CAKDIVKILT TKELKKDSSR APPDDLTKGK RHKVKEFINS 700
YMDKIILKKK QKKALALSSA STRMSSPPPS TSS 733
Length:733
Mass (Da):84,461
Last modified:September 21, 2011 - v2
Checksum:i05436B181E88EFF5
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti594 – 5941A → F in CAA89464. 1 Publication
Sequence conflicti605 – 6051A → S in CAA89464. 1 Publication
Sequence conflicti716 – 7161A → G in CAA89464. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z49444 Genomic DNA. Translation: CAA89464.1.
BK006943 Genomic DNA. Translation: DAA08635.2.
PIRiS56951.
RefSeqiNP_012367.2. NM_001181601.2.

Genome annotation databases

EnsemblFungiiYJL168C; YJL168C; YJL168C.
GeneIDi853271.
KEGGisce:YJL168C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z49444 Genomic DNA. Translation: CAA89464.1 .
BK006943 Genomic DNA. Translation: DAA08635.2 .
PIRi S56951.
RefSeqi NP_012367.2. NM_001181601.2.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1E0N NMR - A 479-505 [» ]
2C5Z NMR - A 620-719 [» ]
ProteinModelPortali P46995.
SMRi P46995. Positions 66-261, 479-505, 622-703.
ModBasei Search...

Protein-protein interaction databases

BioGridi 33591. 392 interactions.
DIPi DIP-2150N.
IntActi P46995. 62 interactions.
MINTi MINT-500810.
STRINGi 4932.YJL168C.

Proteomic databases

MaxQBi