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P46993 (ASG7_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein ASG7
Alternative name(s):
A-specific gene 7 protein
Gene names
Name:ASG7
Ordered Locus Names:YJL170C
ORF Names:J0514
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length209 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for receptor inhibition of inappropriately expressed a-factor receptor (STE3) in MAT a cells. Inhibits signaling by relocalizing the G protein beta-gamma (STE4-STE18) subunit to intracellular mebranes. May also be a mechanism for the down-regulation of the mating pheromone response after the zygotic fusion event, promoting the transition of the new diploid cell to vegetative growth. Ref.4 Ref.5 Ref.6

Subcellular location

Endomembrane system; Multi-pass membrane protein Ref.5.

Induction

Induced by alpha-pheromone. Repressed by the ALPHA2-MCM1 repressor. Ref.3 Ref.5

Sequence caution

The sequence CAA89465.1 differs from that shown. Reason: Frameshift at position 16.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 209209Protein ASG7
PRO_0000203025

Regions

Topological domain1 – 4949Lumenal Potential
Transmembrane50 – 7021Helical; Potential
Topological domain71 – 184114Cytoplasmic Potential
Transmembrane185 – 20521Helical; Potential
Topological domain206 – 2094Lumenal Potential

Amino acid modifications

Modified residue1211Phosphoserine Ref.9
Modified residue1231Phosphoserine Ref.9
Modified residue1251Phosphoserine Ref.9
Modified residue1531Phosphothreonine Ref.9

Sequences

Sequence LengthMass (Da)Tools
P46993 [UniParc].

Last modified April 29, 2008. Version 2.
Checksum: 504DFEDD9063F6AF

FASTA20924,946
        10         20         30         40         50         60 
MTTLASSIEH KTKHLAAPFE NDENPWMKKY CCQCKSCKMS VPVQPWLPRF FVFGILCPVF 

        70         80         90        100        110        120 
WLVNLLAWWF LQYWQPHELE FHDLQEDEYP GFYEYEAITK RTVIPIKEEV LQEIRVMQNF 

       130        140        150        160        170        180 
SDSNSEEYYE SKDGMPSSFL NVNTEQVEDE NDTLKKYRYA FLKKVAHDVL ESHDLLRKTF 

       190        200 
RDWNLRSLLG LLIDSILIIF VVLLCKKSR 

« Hide

References

« Hide 'large scale' references
[1]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Identification of target sites of the alpha2-Mcm1 repressor complex in the yeast genome."
Zhong H., McCord R., Vershon A.K.
Genome Res. 9:1040-1047(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[4]"Asg7p-Ste3p inhibition of pheromone signaling: regulation of the zygotic transition to vegetative growth."
Roth A.F., Nelson B., Boone C., Davis N.G.
Mol. Cell. Biol. 20:8815-8825(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"Localization and signaling of G(beta) subunit Ste4p are controlled by a-factor receptor and the a-specific protein Asg7p."
Kim J., Bortz E., Zhong H., Leeuw T., Leberer E., Vershon A.K., Hirsch J.P.
Mol. Cell. Biol. 20:8826-8835(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INDUCTION BY PHEROMONE, SUBCELLULAR LOCATION.
[6]"Autocrine activation of the pheromone response pathway in matalpha2-cells is attenuated by SST2- and ASG7-dependent mechanisms."
Rivers D.M., Sprague G.F. Jr.
Mol. Genet. Genomics 270:225-233(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Sequencing and comparison of yeast species to identify genes and regulatory elements."
Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.
Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION OF FRAMESHIFT.
[8]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
[9]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-121; SER-123; SER-125 AND THR-153, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z49445 Genomic DNA. Translation: CAA89465.1. Frameshift.
BK006943 Genomic DNA. Translation: DAA08634.1.
PIRS56953.
RefSeqNP_012365.2. NM_001181603.1.

3D structure databases

ProteinModelPortalP46993.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33590. 12 interactions.
DIPDIP-5416N.
IntActP46993. 1 interaction.
MINTMINT-529434.
STRING4932.YJL170C.

Proteomic databases

PaxDbP46993.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJL170C; YJL170C; YJL170C.
GeneID853269.
KEGGsce:YJL170C.

Organism-specific databases

SGDS000003706. ASG7.

Phylogenomic databases

eggNOGNOG74535.
OMAANHHIVE.
OrthoDBEOG7J18CN.

Enzyme and pathway databases

BioCycYEAST:G3O-31607-MONOMER.

Gene expression databases

GenevestigatorP46993.

Family and domain databases

ProtoNetSearch...

Other

NextBio973541.

Entry information

Entry nameASG7_YEAST
AccessionPrimary (citable) accession number: P46993
Secondary accession number(s): D6VW18
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: April 29, 2008
Last modified: May 14, 2014
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD