P46993 (ASG7_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein ASG7

Alternative name(s):
A-specific gene 7 protein

Gene names

Name:	ASG7
Ordered Locus Names:	YJL170C
ORF Names:	J0514

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	209 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for receptor inhibition of inappropriately expressed a-factor receptor (STE3) in MAT a cells. Inhibits signaling by relocalizing the G protein beta-gamma (STE4-STE18) subunit to intracellular mebranes. May also be a mechanism for the down-regulation of the mating pheromone response after the zygotic fusion event, promoting the transition of the new diploid cell to vegetative growth. Ref.4 Ref.5 Ref.6
Subcellular location	Endomembrane system; Multi-pass membrane protein Ref.5.
Induction	Induced by alpha-pheromone. Repressed by the ALPHA2-MCM1 repressor. Ref.3 Ref.5
Sequence caution	The sequence CAA89465.1 differs from that shown. Reason: Frameshift at position 16.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	conjugation with cellular fusion Inferred from expression pattern Ref.3. Source: SGD
Cellular_component	endomembrane system Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay Ref.5. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 209	209	Protein ASG7		PRO_0000203025
Regions
	Topological domain	1 – 49	49	Lumenal Potential
	Transmembrane	50 – 70	21	Helical; Potential
	Topological domain	71 – 184	114	Cytoplasmic Potential
	Transmembrane	185 – 205	21	Helical; Potential
	Topological domain	206 – 209	4	Lumenal Potential
Amino acid modifications
	Modified residue	121	1	Phosphoserine Ref.9
	Modified residue	123	1	Phosphoserine Ref.9
	Modified residue	125	1	Phosphoserine Ref.9
	Modified residue	153	1	Phosphothreonine Ref.9

Sequences

	Sequence		Length	Mass (Da)
	P46993 [UniParc]. Last modified April 29, 2008. Version 2. Checksum: 504DFEDD9063F6AF	FASTA	209	24,946
	10 20 30 40 50 60 MTTLASSIEH KTKHLAAPFE NDENPWMKKY CCQCKSCKMS VPVQPWLPRF FVFGILCPVF 70 80 90 100 110 120 WLVNLLAWWF LQYWQPHELE FHDLQEDEYP GFYEYEAITK RTVIPIKEEV LQEIRVMQNF 130 140 150 160 170 180 SDSNSEEYYE SKDGMPSSFL NVNTEQVEDE NDTLKKYRYA FLKKVAHDVL ESHDLLRKTF 190 200 RDWNLRSLLG LLIDSILIIF VVLLCKKSR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Identification of target sites of the alpha2-Mcm1 repressor complex in the yeast genome." Zhong H., McCord R., Vershon A.K. Genome Res. 9:1040-1047(1999) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[4]	"Asg7p-Ste3p inhibition of pheromone signaling: regulation of the zygotic transition to vegetative growth." Roth A.F., Nelson B., Boone C., Davis N.G. Mol. Cell. Biol. 20:8815-8825(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"Localization and signaling of G(beta) subunit Ste4p are controlled by a-factor receptor and the a-specific protein Asg7p." Kim J., Bortz E., Zhong H., Leeuw T., Leberer E., Vershon A.K., Hirsch J.P. Mol. Cell. Biol. 20:8826-8835(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INDUCTION BY PHEROMONE, SUBCELLULAR LOCATION.
[6]	"Autocrine activation of the pheromone response pathway in matalpha2-cells is attenuated by SST2- and ASG7-dependent mechanisms." Rivers D.M., Sprague G.F. Jr. Mol. Genet. Genomics 270:225-233(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"Sequencing and comparison of yeast species to identify genes and regulatory elements." Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S. Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION OF FRAMESHIFT.
[8]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
[9]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-121; SER-123; SER-125 AND THR-153, MASS SPECTROMETRY. Strain: ADR376.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z49445 Genomic DNA. Translation: CAA89465.1. Frameshift. BK006943 Genomic DNA. Translation: DAA08634.1.
PIR	S56953.
RefSeq	NP_012365.2. NM_001181603.1.
3D structure databases
ProteinModelPortal	P46993.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5416N.
IntAct	P46993. 1 interaction.
MINT	MINT-529434.
STRING	4932.YJL170C.
Proteomic databases
PaxDb	P46993.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJL170C; YJL170C; YJL170C.
GeneID	853269.
KEGG	sce:YJL170C.
Organism-specific databases
SGD	S000003706. ASG7.
Phylogenomic databases
eggNOG	NOG74535.
OMA	APFENDE.
OrthoDB	EOG4GF6R1.
Gene expression databases
Genevestigator	P46993.
GermOnline	YJL170C. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	973541.

Entry information

Entry name

ASG7_YEAST

Accession

Primary (citable) accession number: P46993
Secondary accession number(s): D6VW18

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	April 29, 2008
Last modified:	April 3, 2013
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program