Reviewed, UniProtKB/Swiss-Prot P46993 (ASG7_YEAST)

Names and origin

Protein names

Recommended name:
    Protein ASG7
Alternative name(s):
    A-specific gene 7 protein

Gene names

Name:	ASG7
Ordered Locus Names:	YJL170C
ORF Names:	J0514

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	209 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required for receptor inhibition of inappropriately expressed a-factor receptor (STE3) in MAT a cells. Inhibits signaling by relocalizing the G protein beta-gamma (STE4-STE18) subunit to intracellular mebranes. May also be a mechanism for the down-regulation of the mating pheromone response after the zygotic fusion event, promoting the transition of the new diploid cell to vegetative growth. Ref.3 Ref.4 Ref.5
Subcellular location	Endomembrane system; Multi-pass membrane protein.
Induction	Induced by alpha-pheromone. Repressed by the ALPHA2-MCM1 repressor. Ref.4 Ref.2
Sequence caution	The sequence CAA89465.1 differs from that shown. Reason: Frameshift at position 16.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	conjugation with cellular fusion Ref.2 Inferred from expression pattern. Source: SGD
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Ref.4 Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 209	209	Protein ASG7		PRO_0000203025
Regions
	Topological domain	1 – 49	49	Lumenal Potential
	Transmembrane	50 – 70	21	Potential
	Topological domain	71 – 184	114	Cytoplasmic Potential
	Transmembrane	185 – 205	21	Potential
	Topological domain	206 – 209	4	Lumenal Potential
Amino acid modifications
	Modified residue	121	1	Phosphoserine Ref.8
	Modified residue	123	1	Phosphoserine Ref.8
	Modified residue	125	1	Phosphoserine Ref.8
	Modified residue	153	1	Phosphothreonine Ref.8

Sequences

	Sequence		Length	Mass (Da)
	P46993-1 [UniParc]. Last modified April 29, 2008. Version 2. Checksum: 504DFEDD9063F6AF	FASTA	209	24,946
	10 20 30 40 50 60 MTTLASSIEH KTKHLAAPFE NDENPWMKKY CCQCKSCKMS VPVQPWLPRF FVFGILCPVF 70 80 90 100 110 120 WLVNLLAWWF LQYWQPHELE FHDLQEDEYP GFYEYEAITK RTVIPIKEEV LQEIRVMQNF 130 140 150 160 170 180 SDSNSEEYYE SKDGMPSSFL NVNTEQVEDE NDTLKKYRYA FLKKVAHDVL ESHDLLRKTF 190 200 RDWNLRSLLG LLIDSILIIF VVLLCKKSR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"Identification of target sites of the alpha2-Mcm1 repressor complex in the yeast genome." Zhong H., McCord R., Vershon A.K. Genome Res. 9:1040-1047(1999) [PubMed: 10568744] [Abstract] Cited for: INDUCTION.
[3]	"Asg7p-Ste3p inhibition of pheromone signaling: regulation of the zygotic transition to vegetative growth." Roth A.F., Nelson B., Boone C., Davis N.G. Mol. Cell. Biol. 20:8815-8825(2000) [PubMed: 11073982] [Abstract] Cited for: FUNCTION.
[4]	"Localization and signaling of G(beta) subunit Ste4p are controlled by a-factor receptor and the a-specific protein Asg7p." Kim J., Bortz E., Zhong H., Leeuw T., Leberer E., Vershon A.K., Hirsch J.P. Mol. Cell. Biol. 20:8826-8835(2000) [PubMed: 11073983] [Abstract] Cited for: FUNCTION, INDUCTION BY PHEROMONE, SUBCELLULAR LOCATION.
[5]	"Autocrine activation of the pheromone response pathway in matalpha2-cells is attenuated by SST2- and ASG7-dependent mechanisms." Rivers D.M., Sprague G.F. Jr. Mol. Genet. Genomics 270:225-233(2003) [PubMed: 13680367] [Abstract] Cited for: FUNCTION.
[6]	"Sequencing and comparison of yeast species to identify genes and regulatory elements." Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S. Nature 423:241-254(2003) [PubMed: 12748633] [Abstract] Cited for: IDENTIFICATION OF FRAMESHIFT.
[7]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
[8]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-121; SER-123; SER-125 AND THR-153, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z49445 Genomic DNA. Translation: CAA89465.1. Frameshift.
PIR	S56953.
RefSeq	NP_012365.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:5416N.
IntAct	P46993. 3 interactions.
STRING	P46993.
Genome annotation databases
Ensembl	YJL170C; YJL170C; YJL170C; Saccharomyces cerevisiae. [Genome view]
GeneID	853269.
KEGG	sce:YJL170C.
NMPDR	fig|4932.3.peg.3329.
Organism-specific databases
CYGD	YJL170c.
SGD	S000003706. ASG7.
Phylogenomic databases
OrthoDB	EOG925794
Gene expression databases
ArrayExpress	P46993.
Genevestigator	P46993.
GermOnline	YJL170C. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	973541.

Entry information

Entry name

ASG7_YEAST

Accession

Primary (citable) accession number: P46993

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	April 29, 2008
Last modified:	November 24, 2009
This is version 54 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)