Reviewed, UniProtKB/Swiss-Prot P46990 (RL17B_YEAST)

Names and origin

Protein names

Recommended name:
    60S ribosomal protein L17-B
Alternative name(s):
    L20
    YL17

Gene names

Name:	RPL17B
Synonyms:	RPL20B
Ordered Locus Names:	YJL177W
ORF Names:	J0493

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	184 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subunit structure	Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.3
Subcellular location	Cytoplasm.
Miscellaneous	Present with 21100 molecules/cell in log phase SD medium. Ref.5 There are 2 genes for L17 in yeast.
Sequence similarities	Belongs to the ribosomal protein L22P family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	translation Inferred from direct assay. Source: SGD
Cellular component	cytosolic large ribosomal subunit Inferred from direct assay. Source: SGD
Molecular function	structural constituent of ribosome Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.2
	Chain	2 – 184	183	60S ribosomal protein L17-B		PRO_0000125348
Amino acid modifications
	Modified residue	20	1	Phosphoserine Ref.7
	Modified residue	25	1	Phosphoserine Ref.7
	Modified residue	65	1	Phosphoserine Ref.7 Ref.6 Ref.8
	Modified residue	113	1	Phosphotyrosine Ref.8
	Modified residue	170	1	Phosphoserine Ref.7

Sequences

	Sequence		Length	Mass (Da)
	P46990-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 26F908D90715221D	FASTA	184	20,551
	10 20 30 40 50 60 MARYGATSTN PAKSASARGS YLRVSFKNTR ETAQAINGWE LTKAQKYLDQ VLDHQRAIPF 70 80 90 100 110 120 RRFNSSIGRT AQGKEFGVTK ARWPAKSVKF VQGLLQNAAA NAEAKGLDAT KLYVSHIQVN 130 140 150 160 170 180 QAPKQRRRTY RAHGRINKYE SSPSHIELVV TEKEEAVAKA AEKKVVRLTS RQRGRIAAQK RISA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"Yeast ribosomal proteins. VIII. Isolation of two proteins and sequence characterization of twenty-four proteins from cytoplasmic ribosomes." Otaka E., Higo K., Itoh T. Mol. Gen. Genet. 195:544-546(1984) Cited for: PRELIMINARY PROTEIN SEQUENCE OF 2-41.
[3]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed: 9559554] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-65, MASS SPECTROMETRY.
[7]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-20; SER-25; SER-65 AND SER-170, MASS SPECTROMETRY.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-65 AND TYR-113, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z49452 Genomic DNA. Translation: CAA89472.1.
PIR	S56960.
RefSeq	NP_012358.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P46990.
Proteomic databases
PeptideAtlas	P46990.
PRIDE	P46990.
Genome annotation databases
Ensembl	YJL177W; YJL177W; YJL177W; Saccharomyces cerevisiae. [Genome view]
GeneID	853262.
KEGG	sce:YJL177W.
NMPDR	fig|4932.3.peg.3322.
Organism-specific databases
CYGD	YJL177w.
SGD	S000003713. RPL17B.
Phylogenomic databases
HOGENOM	P46990.
OMA	KECVPFR
OrthoDB	EOG9Q2FZ3
Gene expression databases
ArrayExpress	P46990.
Genevestigator	P46990.
GermOnline	YJL177W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001063. Ribosomal_L22. IPR018260. Ribosomal_L22/L17_CS. IPR005721. Ribosomal_L22/L17_euk/arc. [Graphical view]
PANTHER	PTHR11593. Ribosomal_L22/17. 1 hit.
Pfam	PF00237. Ribosomal_L22. 1 hit. [Graphical view]
TIGRFAMs	TIGR01038. L22_arch. 1 hit.
PROSITE	PS00464. RIBOSOMAL_L22. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	973523.

Entry information

Entry name

RL17B_YEAST

Accession

Primary (citable) accession number: P46990

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	January 23, 2007
Last modified:	November 24, 2009
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)