P46989 (ATG27_YEAST) Reviewed, UniProtKB/Swiss-Prot
Last modified
December 14, 2011.
Version 86.
History...
Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Autophagy-related protein 27 Alternative name(s): Enhancer of VPS34 missorting protein 1 | ||||||||
| Gene names |
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| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | ||||||||
| Taxonomic identifier | 559292 [NCBI] | ||||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 271 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Effector of VPS34 phosphatidylinositol 3-phosphate kinase signaling. Regulates the cytoplasm to vacuole transport (Cvt) vesicle formation. Required for autophagy-dependent cycling of ATG9. Ref.5 Ref.9 |
| Subcellular location | Cytoplasmic vesicle membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Mitochondrion membrane; Single-pass membrane protein. Note: Cycles among the pre-autophagosomal structure (PAS), mitochondria and Golgi. Ref.5 Ref.7 Ref.9 |
| Miscellaneous | Present with 8970 molecules/cell in log phase SD medium. Ref.8 |
| Sequence similarities | Belongs to the ATG27 family. |
| Sequence caution | The sequence AAS56577.1 differs from that shown. Reason: Frameshift at position 68. The sequence CAA89473.1 differs from that shown. Reason: Frameshift at position 68. |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| itself | 1 | EBI-26164,EBI-26164 | ||
| AGP1 | P25376 | 1 | EBI-26164,EBI-2357 | |
| AKR1 | P39010 | 1 | EBI-26164,EBI-2421 | |
| COT1 | P32798 | 1 | EBI-26164,EBI-5006 | |
| CTR2 | P38865 | 1 | EBI-26164,EBI-5268 | |
| EMP47 | P43555 | 1 | EBI-26164,EBI-6439 | |
| FMP42 | Q04991 | 1 | EBI-26164,EBI-27478 | |
| FTH1 | P38310 | 1 | EBI-26164,EBI-20959 | |
| OST1 | P41543 | 1 | EBI-26164,EBI-12651 | |
| PEP1 | P32319 | 1 | EBI-26164,EBI-13123 | |
| PMC1 | P38929 | 1 | EBI-26164,EBI-3097 | |
| PMP2 | P40975 | 1 | EBI-26164,EBI-2043041 | |
| PMP3 | P87284 | 1 | EBI-26164,EBI-13555 | |
| RBD2 | Q12270 | 1 | EBI-26164,EBI-31471 | |
| SAC1 | P32368 | 1 | EBI-26164,EBI-16210 | |
| SFT2 | P38166 | 1 | EBI-26164,EBI-17046 | |
| SNX3 | Q08826 | 1 | EBI-26164,EBI-17603 | |
| SPF1 | P39986 | 1 | EBI-26164,EBI-3128 | |
| TMS1 | Q12116 | 1 | EBI-26164,EBI-19306 | |
| TVP15 | Q03860 | 1 | EBI-26164,EBI-37537 | |
| VPS16 | Q03308 | 1 | EBI-26164,EBI-20355 | |
| VPS17 | P32913 | 1 | EBI-26164,EBI-20366 | |
| VPS5 | Q92331 | 1 | EBI-26164,EBI-20483 | |
| VPS68 | Q12016 | 1 | EBI-26164,EBI-29309 | |
| YML018C | Q03730 | 1 | EBI-26164,EBI-27739 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 19 | 19 | Potential | ||||||
| Chain | 20 – 271 | 252 | Autophagy-related protein 27 | PRO_0000001780 | |||||
Regions | |||||||||
| Topological domain | 20 – 199 | 180 | Lumenal Ref.9 | ||||||
| Transmembrane | 200 – 220 | 21 | Helical; Potential | ||||||
| Topological domain | 221 – 271 | 51 | Cytoplasmic Ref.9 | ||||||
Experimental info | |||||||||
| Mutagenesis | 188 – 193 | 6 | KKPAKK → AAPAAA: Abolishes the binding of phosphatidylinositol 3-phosphate and the cytoplasm to vacuole transport. Ref.5 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. Karpfinger-Hartl L.EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679. |
| [2] | Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c. |
| [3] | "Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J.Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [4] | "Reinvestigation of the Saccharomyces cerevisiae genome annotation by comparison to the genome of a related fungus: Ashbya gossypii." Brachat S., Dietrich F.S., Voegeli S., Zhang Z., Stuart L., Lerch A., Gates K., Gaffney T.D., Philippsen P. Genome Biol. 4:R45.1-R45.13(2003) [PubMed: 12844361] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 4-103. Strain: ATCC 204511 / S288c / AB972. |
| [5] | "Novel PtdIns(3)P-binding protein Etf1 functions as an effector of the Vps34 PtdIns 3-kinase in autophagy." Wurmser A.E., Emr S.D. J. Cell Biol. 158:761-772(2002) [PubMed: 12186856] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF 188-LYS--LYS-193. |
| [6] | "A unified nomenclature for yeast autophagy-related genes." Klionsky D.J., Cregg J.M., Dunn W.A. Jr., Emr S.D., Sakai Y., Sandoval I.V., Sibirny A., Subramani S., Thumm M., Veenhuis M., Ohsumi Y. Dev. Cell 5:539-545(2003) [PubMed: 14536056] [Abstract] Cited for: NOMENCLATURE. |
| [7] | "Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. |
| [8] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| [9] | "Atg27 is required for autophagy-dependent cycling of Atg9." Yen W.-L., Legakis J.E., Nair U., Klionsky D.J. Mol. Biol. Cell 18:581-593(2007) [PubMed: 17135291] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | Z49453 Genomic DNA. Translation: CAA89473.1. Frameshift. AY558251 Genomic DNA. Translation: AAS56577.1. Frameshift. AY260894 Genomic DNA. Translation: AAP21762.1. BK006943 Genomic DNA. Translation: DAA08627.1. |
| PIR | S56961. |
| RefSeq | NP_012357.2. NM_001181611.1. |
3D structure databases | |
| ProteinModelPortal | P46989. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP-1606N. |
| IntAct | P46989. 27 interactions. |
| MINT | MINT-400196. |
| STRING | P46989. |
Proteomic databases | |
| PeptideAtlas | P46989. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblFungi | YJL178C; YJL178C; YJL178C. |
| GeneID | 853261. |
| KEGG | sce:YJL178C. |
| NMPDR | fig|4932.3.peg.3321. |
Organism-specific databases | |
| CYGD | YJL178c. |
| SGD | S000003714. ATG27. |
Phylogenomic databases | |
| eggNOG | fuNOG11027. |
| HOGENOM | HBG202279. |
| OMA | WFTWIFI. |
| OrthoDB | EOG4T7CCT. |
Gene expression databases | |
| ArrayExpress | P46989. |
| Genevestigator | P46989. |
| GermOnline | YJL178C. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR018939. Autophagy-rel_prot_27. [Graphical view] |
| Pfam | PF09451. ATG27. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 973520. |
Entry information
| Entry name | ATG27_YEAST | ||||||||
| Accession | Primary (citable) accession number: P46989 Secondary accession number(s): D6VW11, Q86ZR9 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Fungal Protein Annotation Program | ||||||||
Relevant documents
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome X Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names |
| SIMILARITY comments Index of protein domains and families |

Clusters with