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P46989

- ATG27_YEAST

UniProt

P46989 - ATG27_YEAST

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Protein

Autophagy-related protein 27

Gene

ATG27

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Effector of VPS34 phosphatidylinositol 3-phosphate kinase signaling. Regulates the cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a role in ATG protein retrieval from the pre-autophagosomal structure (PAS) and is especially required for autophagy-dependent cycling of ATG9.4 Publications

GO - Molecular functioni

  1. phosphatidylinositol-3-phosphate binding Source: SGD

GO - Biological processi

  1. autophagic vacuole assembly Source: SGD
  2. CVT pathway Source: SGD
  3. peroxisome degradation Source: SGD
  4. protein localization to pre-autophagosomal structure Source: SGD
  5. vesicle organization Source: SGD
Complete GO annotation...

Keywords - Biological processi

Autophagy, Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31613-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Autophagy-related protein 27
Alternative name(s):
Enhancer of VPS34 missorting protein 1
Gene namesi
Name:ATG27
Synonyms:ETF1
Ordered Locus Names:YJL178C
ORF Names:J0490
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

CYGDiYJL178c.
SGDiS000003714. ATG27.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini20 – 199180Lumenal1 PublicationAdd
BLAST
Transmembranei200 – 22021HelicalSequence AnalysisAdd
BLAST
Topological domaini221 – 27151Cytoplasmic1 PublicationAdd
BLAST

GO - Cellular componenti

  1. cytoplasmic vesicle Source: UniProtKB-KW
  2. integral component of membrane Source: UniProtKB-KW
  3. membrane Source: SGD
  4. mitochondrion Source: SGD
  5. pre-autophagosomal structure Source: SGD
  6. trans-Golgi network Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Golgi apparatus, Membrane, Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi188 – 1936KKPAKK → AAPAAA: Abolishes the binding of phosphatidylinositol 3-phosphate and the cytoplasm to vacuole transport. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence AnalysisAdd
BLAST
Chaini20 – 271252Autophagy-related protein 27PRO_0000001780Add
BLAST

Proteomic databases

MaxQBiP46989.
PaxDbiP46989.
PeptideAtlasiP46989.

Expressioni

Gene expression databases

GenevestigatoriP46989.

Interactioni

Subunit structurei

Forms a complex with ATG9 and ATG23.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself1EBI-26164,EBI-26164
AGP1P253761EBI-26164,EBI-2357
AKR1P390101EBI-26164,EBI-2421
COT1P327981EBI-26164,EBI-5006
CTR2P388651EBI-26164,EBI-5268
EMP47P435551EBI-26164,EBI-6439
FMP42Q049911EBI-26164,EBI-27478
FTH1P383101EBI-26164,EBI-20959
OST1P415431EBI-26164,EBI-12651
PEP1P323191EBI-26164,EBI-13123
PMC1P389291EBI-26164,EBI-3097
PMP2P409751EBI-26164,EBI-2043041
PMP3P872841EBI-26164,EBI-13555
RBD2Q122701EBI-26164,EBI-31471
SAC1P323681EBI-26164,EBI-16210
SFT2P381661EBI-26164,EBI-17046
SNX3Q088261EBI-26164,EBI-17603
SPF1P399861EBI-26164,EBI-3128
TMS1Q121161EBI-26164,EBI-19306
TVP15Q038601EBI-26164,EBI-37537
VPS16Q033081EBI-26164,EBI-20355
VPS17P329131EBI-26164,EBI-20366
VPS5Q923311EBI-26164,EBI-20483
VPS68Q120161EBI-26164,EBI-29309
YML018CQ037301EBI-26164,EBI-27739

Protein-protein interaction databases

BioGridi33583. 59 interactions.
DIPiDIP-1606N.
IntActiP46989. 21 interactions.
MINTiMINT-400196.
STRINGi4932.YJL178C.

Structurei

3D structure databases

ProteinModelPortaliP46989.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATG27 family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG40555.
HOGENOMiHOG000034146.
InParanoidiP46989.
OMAiGAWFQYN.
OrthoDBiEOG7KH9W8.

Family and domain databases

InterProiIPR018939. Autophagy-rel_prot_27.
[Graphical view]
PfamiPF09451. ATG27. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P46989-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVSKTWICGF ISIITVVQAL SCEKHDVLKK YQVGKFSSLT STERDTPPST
60 70 80 90 100
TIEKWWINVC EEHNVEPPEE CKKNDMLCGL TDVILPGKDA ITTQIIDFDK
110 120 130 140 150
NIGFNVEETE SALTLTLKGA TWGANSFDAK LEFQCNDNMK QDELTSHTWA
160 170 180 190 200
DKSIQLTLKG PSGCLKSKDD DKKNGDGDNG KDGDSEGKKP AKKAGGTSWF
210 220 230 240 250
TWLFLYALLF TLIYLMVVSF LNTRGGSFQD FRAEFIQRST QFLTSLPEFC
260 270
KEVVSRILGR STAQRGGYSA V
Length:271
Mass (Da):30,201
Last modified:March 15, 2005 - v2
Checksum:iBD2F3BF4C5904ED2
GO

Sequence cautioni

The sequence AAS56577.1 differs from that shown. Reason: Frameshift at position 68. Curated
The sequence CAA89473.1 differs from that shown. Reason: Frameshift at position 68. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49453 Genomic DNA. Translation: CAA89473.1. Frameshift.
AY558251 Genomic DNA. Translation: AAS56577.1. Frameshift.
AY260894 Genomic DNA. Translation: AAP21762.1.
BK006943 Genomic DNA. Translation: DAA08627.1.
PIRiS56961.
RefSeqiNP_012357.2. NM_001181611.1.

Genome annotation databases

EnsemblFungiiYJL178C; YJL178C; YJL178C.
GeneIDi853261.
KEGGisce:YJL178C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49453 Genomic DNA. Translation: CAA89473.1 . Frameshift.
AY558251 Genomic DNA. Translation: AAS56577.1 . Frameshift.
AY260894 Genomic DNA. Translation: AAP21762.1 .
BK006943 Genomic DNA. Translation: DAA08627.1 .
PIRi S56961.
RefSeqi NP_012357.2. NM_001181611.1.

3D structure databases

ProteinModelPortali P46989.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33583. 59 interactions.
DIPi DIP-1606N.
IntActi P46989. 21 interactions.
MINTi MINT-400196.
STRINGi 4932.YJL178C.

Proteomic databases

MaxQBi P46989.
PaxDbi P46989.
PeptideAtlasi P46989.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YJL178C ; YJL178C ; YJL178C .
GeneIDi 853261.
KEGGi sce:YJL178C.

Organism-specific databases

CYGDi YJL178c.
SGDi S000003714. ATG27.

Phylogenomic databases

eggNOGi NOG40555.
HOGENOMi HOG000034146.
InParanoidi P46989.
OMAi GAWFQYN.
OrthoDBi EOG7KH9W8.

Enzyme and pathway databases

BioCyci YEAST:G3O-31613-MONOMER.

Miscellaneous databases

NextBioi 973520.

Gene expression databases

Genevestigatori P46989.

Family and domain databases

InterProi IPR018939. Autophagy-rel_prot_27.
[Graphical view ]
Pfami PF09451. ATG27. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Reinvestigation of the Saccharomyces cerevisiae genome annotation by comparison to the genome of a related fungus: Ashbya gossypii."
    Brachat S., Dietrich F.S., Voegeli S., Zhang Z., Stuart L., Lerch A., Gates K., Gaffney T.D., Philippsen P.
    Genome Biol. 4:R45.1-R45.13(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 4-103.
    Strain: ATCC 204511 / S288c / AB972.
  5. "Novel PtdIns(3)P-binding protein Etf1 functions as an effector of the Vps34 PtdIns 3-kinase in autophagy."
    Wurmser A.E., Emr S.D.
    J. Cell Biol. 158:761-772(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF 188-LYS--LYS-193.
  6. Cited for: NOMENCLATURE.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "A cycling protein complex required for selective autophagy."
    Legakis J.E., Yen W.L., Klionsky D.J.
    Autophagy 3:422-432(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, IDENTIFICATION IN THE ATG9-ATG23-ATG27 COMPLEX, FUNCTION.
  10. "Atg27 is required for autophagy-dependent cycling of Atg9."
    Yen W.-L., Legakis J.E., Nair U., Klionsky D.J.
    Mol. Biol. Cell 18:581-593(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY.
  11. "Atg9 vesicles recruit vesicle-tethering proteins Trs85 and Ypt1 to the autophagosome formation site."
    Kakuta S., Yamamoto H., Negishi L., Kondo-Kakuta C., Hayashi N., Ohsumi Y.
    J. Biol. Chem. 287:44261-44269(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  12. "Atg9 vesicles are an important membrane source during early steps of autophagosome formation."
    Yamamoto H., Kakuta S., Watanabe T.M., Kitamura A., Sekito T., Kondo-Kakuta C., Ichikawa R., Kinjo M., Ohsumi Y.
    J. Cell Biol. 198:219-233(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiATG27_YEAST
AccessioniPrimary (citable) accession number: P46989
Secondary accession number(s): D6VW11, Q86ZR9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: March 15, 2005
Last modified: October 29, 2014
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 8970 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3