Autophagy-related protein 27 precursor - Saccharomyces cerevisiae (Baker's yeast)

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Reviewed, UniProtKB/Swiss-Prot P46989 (ATG27_YEAST)

Last modified November 24, 2009. Version 69. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
Autophagy-related protein 27
Alternative name(s):
Enhancer of VPS34 missorting protein 1

Gene names

Name:	ATG27
Synonyms:	ETF1
Ordered Locus Names:	YJL178C
ORF Names:	J0490

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length	271 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Effector of VPS34 phosphatidylinositol 3-phosphate kinase signaling. Regulates the cytoplasm to vacuole transport (Cvt) vesicle formation. Required for autophagy-dependent cycling of ATG9. Ref.4 Ref.8
Subcellular location	Cytoplasmic vesicle membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Mitochondrion membrane; Single-pass membrane protein. Note: Cycles among the pre-autophagosomal structure (PAS), mitochondria and Golgi. Ref.4 Ref.8 Ref.6
Miscellaneous	Present with 8970 molecules/cell in log phase SD medium. Ref.7
Sequence similarities	Belongs to the ATG27 family.
Sequence caution	The sequence AAS56577.1 differs from that shown. Reason: Frameshift at position 68. The sequence CAA89473.1 differs from that shown. Reason: Frameshift at position 68.

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Ontologies

Keywords
Biological process	Autophagy Protein transport Transport
Cellular component	Cytoplasmic vesicle Golgi apparatus Membrane Mitochondrion
Domain	Signal Transmembrane
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	CVT pathway Ref.8 Inferred from mutant phenotype. Source: SGD autophagic vacuole formation Ref.8 Inferred from mutant phenotype. Source: SGD peroxisome degradation Ref.8 Inferred from mutant phenotype. Source: SGD protein localization to pre-autophagosomal structure Ref.8 Inferred from mutant phenotype. Source: SGD
Cellular component	cytoplasmic vesicle Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrion Ref.8 Inferred from direct assay. Source: SGD pre-autophagosomal structure Ref.8 Inferred from direct assay. Source: SGD trans-Golgi network Inferred from direct assay. Source: SGD
Molecular function	identical protein binding Inferred from physical interaction. Source: IntAct phosphatidylinositol 3-phosphate binding Ref.4 Inferred from direct assay. Source: SGD
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct
itself		1	EBI-26164,EBI-26164
	Q03730	1	EBI-26164,EBI-27739
AGP1	P25376	1	EBI-26164,EBI-2357
AKR1	P39010	1	EBI-26164,EBI-2421
COT1	P32798	1	EBI-26164,EBI-5006
CTR2	P38865	1	EBI-26164,EBI-5268
EMP47	P43555	1	EBI-26164,EBI-6439
FMP42	Q04991	1	EBI-26164,EBI-27478
FTH1	P38310	1	EBI-26164,EBI-20959
OST1	P41543	1	EBI-26164,EBI-12651
PEP1	P32319	1	EBI-26164,EBI-13123
PMC1	P38929	1	EBI-26164,EBI-3097
PMP2	P40975	1	EBI-26164,EBI-2043041
PMP3	P87284	1	EBI-26164,EBI-13555
RBD2	Q12270	1	EBI-26164,EBI-31471
SAC1	P32368	1	EBI-26164,EBI-16210
SFT2	P38166	1	EBI-26164,EBI-17046
SNX3	Q08826	1	EBI-26164,EBI-17603
SPF1	P39986	1	EBI-26164,EBI-3128
TMS1	Q12116	1	EBI-26164,EBI-19306
TVP15	Q03860	1	EBI-26164,EBI-37537
VPS16	Q03308	1	EBI-26164,EBI-20355
VPS17	P32913	1	EBI-26164,EBI-20366
VPS5	Q92331	1	EBI-26164,EBI-20483
VPS68	Q12016	1	EBI-26164,EBI-29309

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 19

Potential

Chain

20 – 271

252

Autophagy-related protein 27

PRO_0000001780

Regions

Topological domain

20 – 199

180

Lumenal Ref.8

Transmembrane

200 – 220

Potential

Topological domain

221 – 271

Cytoplasmic Ref.8

Experimental info

Mutagenesis

188 – 193

KKPAKK → AAPAAA: Abolishes the binding of phosphatidylinositol 3-phosphate and the cytoplasm to vacuole transport. Ref.4

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Sequences

Sequence

Length

Mass (Da)

Tools

P46989-1 [UniParc].

Last modified March 15, 2005. Version 2.
Checksum: BD2F3BF4C5904ED2
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FASTA

271

30,201

        10         20         30         40         50         60 
MVSKTWICGF ISIITVVQAL SCEKHDVLKK YQVGKFSSLT STERDTPPST TIEKWWINVC 

        70         80         90        100        110        120 
EEHNVEPPEE CKKNDMLCGL TDVILPGKDA ITTQIIDFDK NIGFNVEETE SALTLTLKGA 

       130        140        150        160        170        180 
TWGANSFDAK LEFQCNDNMK QDELTSHTWA DKSIQLTLKG PSGCLKSKDD DKKNGDGDNG 

       190        200        210        220        230        240 
KDGDSEGKKP AKKAGGTSWF TWLFLYALLF TLIYLMVVSF LNTRGGSFQD FRAEFIQRST 

       250        260        270 
QFLTSLPEFC KEVVSRILGR STAQRGGYSA V

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Reinvestigation of the Saccharomyces cerevisiae genome annotation by comparison to the genome of a related fungus: Ashbya gossypii." Brachat S., Dietrich F.S., Voegeli S., Zhang Z., Stuart L., Lerch A., Gates K., Gaffney T.D., Philippsen P. Genome Biol. 4:R45.1-R45.13(2003) [PubMed: 12844361] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 4-103. Strain: ATCC 204511 / S288c / AB972.
[4]	"Novel PtdIns(3)P-binding protein Etf1 functions as an effector of the Vps34 PtdIns 3-kinase in autophagy." Wurmser A.E., Emr S.D. J. Cell Biol. 158:761-772(2002) [PubMed: 12186856] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF 188-LYS--LYS-193.
[5]	"A unified nomenclature for yeast autophagy-related genes." Klionsky D.J., Cregg J.M., Dunn W.A. Jr., Emr S.D., Sakai Y., Sandoval I.V., Sibirny A., Subramani S., Thumm M., Veenhuis M., Ohsumi Y. Dev. Cell 5:539-545(2003) [PubMed: 14536056] [Abstract] Cited for: NOMENCLATURE.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Atg27 is required for autophagy-dependent cycling of Atg9." Yen W.-L., Legakis J.E., Nair U., Klionsky D.J. Mol. Biol. Cell 18:581-593(2007) [PubMed: 17135291] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	Z49453 Genomic DNA. Translation: CAA89473.1. Frameshift. AY558251 Genomic DNA. Translation: AAS56577.1. Frameshift. AY260894 Genomic DNA. Translation: AAP21762.1.
PIR	S56961.
RefSeq	NP_012357.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1606N.
IntAct	P46989. 34 interactions.
STRING	P46989.
Proteomic databases
PeptideAtlas	P46989.
Genome annotation databases
Ensembl	YJL178C; YJL178C; YJL178C; Saccharomyces cerevisiae. [Genome view]
GeneID	853261.
KEGG	sce:YJL178C.
NMPDR	fig\|4932.3.peg.3321.
Organism-specific databases
CYGD	YJL178c.
SGD	S000003714. ATG27.
Phylogenomic databases
HOGENOM	P46989.
OMA	WFTWIFI
OrthoDB	EOG9F4TTF
Gene expression databases
ArrayExpress	P46989.
Genevestigator	P46989.
GermOnline	YJL178C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR018939. Autophagy-rel_prot_27. [Graphical view]
Pfam	PF09451. ATG27. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	973520.

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Entry information

Entry name

ATG27_YEAST

Accession

Primary (citable) accession number: P46989
Secondary accession number(s): Q86ZR9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	March 15, 2005
Last modified:	November 24, 2009
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents