Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Autophagy-related protein 27

Gene

ATG27

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Effector of VPS34 phosphatidylinositol 3-phosphate kinase signaling. Regulates the cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a role in ATG protein retrieval from the pre-autophagosomal structure (PAS) and is especially required for autophagy-dependent cycling of ATG9.4 Publications

GO - Molecular functioni

  • phosphatidylinositol-3-phosphate binding Source: SGD

GO - Biological processi

  • autophagosome assembly Source: SGD
  • CVT pathway Source: SGD
  • pexophagy Source: SGD
  • protein localization to pre-autophagosomal structure Source: SGD
  • vesicle organization Source: SGD
Complete GO annotation...

Keywords - Biological processi

Autophagy, Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31613-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Autophagy-related protein 27
Alternative name(s):
Enhancer of VPS34 missorting protein 1
Gene namesi
Name:ATG27
Synonyms:ETF1
Ordered Locus Names:YJL178C
ORF Names:J0490
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJL178C.
SGDiS000003714. ATG27.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini20 – 199180Lumenal1 PublicationAdd
BLAST
Transmembranei200 – 22021HelicalSequence analysisAdd
BLAST
Topological domaini221 – 27151Cytoplasmic1 PublicationAdd
BLAST

GO - Cellular componenti

  • cytoplasmic vesicle membrane Source: UniProtKB-SubCell
  • Golgi membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: SGD
  • mitochondrial membrane Source: UniProtKB-SubCell
  • mitochondrion Source: SGD
  • pre-autophagosomal structure Source: SGD
  • pre-autophagosomal structure membrane Source: UniProtKB-SubCell
  • trans-Golgi network Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Golgi apparatus, Membrane, Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi188 – 1936KKPAKK → AAPAAA: Abolishes the binding of phosphatidylinositol 3-phosphate and the cytoplasm to vacuole transport. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence analysisAdd
BLAST
Chaini20 – 271252Autophagy-related protein 27PRO_0000001780Add
BLAST

Proteomic databases

MaxQBiP46989.
TopDownProteomicsiP46989.

Interactioni

Subunit structurei

Forms a complex with ATG9 and ATG23.1 Publication

Protein-protein interaction databases

BioGridi33583. 105 interactions.
DIPiDIP-1606N.
IntActiP46989. 21 interactions.
MINTiMINT-400196.

Structurei

3D structure databases

ProteinModelPortaliP46989.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATG27 family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000034146.
InParanoidiP46989.
OMAiWFTWIFI.
OrthoDBiEOG092C478W.

Family and domain databases

InterProiIPR018939. Autophagy-rel_prot_27.
[Graphical view]
PfamiPF09451. ATG27. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P46989-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSKTWICGF ISIITVVQAL SCEKHDVLKK YQVGKFSSLT STERDTPPST
60 70 80 90 100
TIEKWWINVC EEHNVEPPEE CKKNDMLCGL TDVILPGKDA ITTQIIDFDK
110 120 130 140 150
NIGFNVEETE SALTLTLKGA TWGANSFDAK LEFQCNDNMK QDELTSHTWA
160 170 180 190 200
DKSIQLTLKG PSGCLKSKDD DKKNGDGDNG KDGDSEGKKP AKKAGGTSWF
210 220 230 240 250
TWLFLYALLF TLIYLMVVSF LNTRGGSFQD FRAEFIQRST QFLTSLPEFC
260 270
KEVVSRILGR STAQRGGYSA V
Length:271
Mass (Da):30,201
Last modified:March 15, 2005 - v2
Checksum:iBD2F3BF4C5904ED2
GO

Sequence cautioni

The sequence AAS56577 differs from that shown. Reason: Frameshift at position 68. Curated
The sequence CAA89473 differs from that shown. Reason: Frameshift at position 68. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49453 Genomic DNA. Translation: CAA89473.1. Frameshift.
AY558251 Genomic DNA. Translation: AAS56577.1. Frameshift.
AY260894 Genomic DNA. Translation: AAP21762.1.
BK006943 Genomic DNA. Translation: DAA08627.1.
PIRiS56961.
RefSeqiNP_012357.2. NM_001181611.1.

Genome annotation databases

EnsemblFungiiYJL178C; YJL178C; YJL178C.
GeneIDi853261.
KEGGisce:YJL178C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49453 Genomic DNA. Translation: CAA89473.1. Frameshift.
AY558251 Genomic DNA. Translation: AAS56577.1. Frameshift.
AY260894 Genomic DNA. Translation: AAP21762.1.
BK006943 Genomic DNA. Translation: DAA08627.1.
PIRiS56961.
RefSeqiNP_012357.2. NM_001181611.1.

3D structure databases

ProteinModelPortaliP46989.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33583. 105 interactions.
DIPiDIP-1606N.
IntActiP46989. 21 interactions.
MINTiMINT-400196.

Proteomic databases

MaxQBiP46989.
TopDownProteomicsiP46989.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJL178C; YJL178C; YJL178C.
GeneIDi853261.
KEGGisce:YJL178C.

Organism-specific databases

EuPathDBiFungiDB:YJL178C.
SGDiS000003714. ATG27.

Phylogenomic databases

HOGENOMiHOG000034146.
InParanoidiP46989.
OMAiWFTWIFI.
OrthoDBiEOG092C478W.

Enzyme and pathway databases

BioCyciYEAST:G3O-31613-MONOMER.

Miscellaneous databases

PROiP46989.

Family and domain databases

InterProiIPR018939. Autophagy-rel_prot_27.
[Graphical view]
PfamiPF09451. ATG27. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiATG27_YEAST
AccessioniPrimary (citable) accession number: P46989
Secondary accession number(s): D6VW11, Q86ZR9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: March 15, 2005
Last modified: September 7, 2016
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 8970 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.