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P46989 (ATG27_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Autophagy-related protein 27
Alternative name(s):
Enhancer of VPS34 missorting protein 1
Gene names
Name:ATG27
Synonyms:ETF1
Ordered Locus Names:YJL178C
ORF Names:J0490
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length271 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Effector of VPS34 phosphatidylinositol 3-phosphate kinase signaling. Regulates the cytoplasm to vacuole transport (Cvt) vesicle formation. Required for autophagy-dependent cycling of ATG9. Ref.5 Ref.9

Subcellular location

Cytoplasmic vesicle membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Mitochondrion membrane; Single-pass membrane protein. Note: Cycles among the pre-autophagosomal structure (PAS), mitochondria and Golgi. Ref.5 Ref.7 Ref.9

Miscellaneous

Present with 8970 molecules/cell in log phase SD medium. Ref.8

Sequence similarities

Belongs to the ATG27 family.

Sequence caution

The sequence AAS56577.1 differs from that shown. Reason: Frameshift at position 68.

The sequence CAA89473.1 differs from that shown. Reason: Frameshift at position 68.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Chain20 – 271252Autophagy-related protein 27
PRO_0000001780

Regions

Topological domain20 – 199180Lumenal Ref.9
Transmembrane200 – 22021Helical; Potential
Topological domain221 – 27151Cytoplasmic Ref.9

Experimental info

Mutagenesis188 – 1936KKPAKK → AAPAAA: Abolishes the binding of phosphatidylinositol 3-phosphate and the cytoplasm to vacuole transport. Ref.5

Sequences

Sequence LengthMass (Da)Tools
P46989 [UniParc].

Last modified March 15, 2005. Version 2.
Checksum: BD2F3BF4C5904ED2

FASTA27130,201
        10         20         30         40         50         60 
MVSKTWICGF ISIITVVQAL SCEKHDVLKK YQVGKFSSLT STERDTPPST TIEKWWINVC 

        70         80         90        100        110        120 
EEHNVEPPEE CKKNDMLCGL TDVILPGKDA ITTQIIDFDK NIGFNVEETE SALTLTLKGA 

       130        140        150        160        170        180 
TWGANSFDAK LEFQCNDNMK QDELTSHTWA DKSIQLTLKG PSGCLKSKDD DKKNGDGDNG 

       190        200        210        220        230        240 
KDGDSEGKKP AKKAGGTSWF TWLFLYALLF TLIYLMVVSF LNTRGGSFQD FRAEFIQRST 

       250        260        270 
QFLTSLPEFC KEVVSRILGR STAQRGGYSA V

« Hide

References

« Hide 'large scale' references
[1]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Reinvestigation of the Saccharomyces cerevisiae genome annotation by comparison to the genome of a related fungus: Ashbya gossypii."
Brachat S., Dietrich F.S., Voegeli S., Zhang Z., Stuart L., Lerch A., Gates K., Gaffney T.D., Philippsen P.
Genome Biol. 4:R45.1-R45.13(2003) [PubMed: 12844361] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 4-103.
Strain: ATCC 204511 / S288c / AB972.
[5]"Novel PtdIns(3)P-binding protein Etf1 functions as an effector of the Vps34 PtdIns 3-kinase in autophagy."
Wurmser A.E., Emr S.D.
J. Cell Biol. 158:761-772(2002) [PubMed: 12186856] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF 188-LYS--LYS-193.
[6]"A unified nomenclature for yeast autophagy-related genes."
Klionsky D.J., Cregg J.M., Dunn W.A. Jr., Emr S.D., Sakai Y., Sandoval I.V., Sibirny A., Subramani S., Thumm M., Veenhuis M., Ohsumi Y.
Dev. Cell 5:539-545(2003) [PubMed: 14536056] [Abstract]
Cited for: NOMENCLATURE.
[7]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed: 14562095] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"Atg27 is required for autophagy-dependent cycling of Atg9."
Yen W.-L., Legakis J.E., Nair U., Klionsky D.J.
Mol. Biol. Cell 18:581-593(2007) [PubMed: 17135291] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		Z49453 Genomic DNA. Translation: CAA89473.1. Frameshift.
AY558251 Genomic DNA. Translation: AAS56577.1. Frameshift.
AY260894 Genomic DNA. Translation: AAP21762.1.
BK006943 Genomic DNA. Translation: DAA08627.1.
PIRS56961.
RefSeqNP_012357.2. NM_001181611.1.

3D structure databases

ProteinModelPortalP46989.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-1606N.
IntActP46989. 27 interactions.
MINTMINT-400196.
STRINGP46989.

Proteomic databases

PeptideAtlasP46989.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJL178C; YJL178C; YJL178C.
GeneID853261.
KEGGsce:YJL178C.
NMPDRfig|4932.3.peg.3321.

Organism-specific databases

CYGDYJL178c.
SGDS000003714. ATG27.

Phylogenomic databases

eggNOGfuNOG11027.
HOGENOMHBG202279.
OMAWFTWIFI.
OrthoDBEOG4T7CCT.

Gene expression databases

ArrayExpressP46989.
GenevestigatorP46989.
GermOnlineYJL178C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR018939. Autophagy-rel_prot_27.
[Graphical view]
PfamPF09451. ATG27. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio973520.

Entry information

Entry nameATG27_YEAST
AccessionPrimary (citable) accession number: P46989
Secondary accession number(s): D6VW11, Q86ZR9
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: March 15, 2005
Last modified: December 14, 2011
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents