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Protein

Autophagy-related protein 27

Gene

ATG27

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Effector of VPS34 phosphatidylinositol 3-phosphate kinase signaling. Regulates the cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a role in ATG protein retrieval from the pre-autophagosomal structure (PAS) and is especially required for autophagy-dependent cycling of ATG9.4 Publications

Miscellaneous

Present with 8970 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • phosphatidylinositol-3-phosphate binding Source: SGD

GO - Biological processi

  • autophagosome assembly Source: SGD
  • autophagy of peroxisome Source: SGD
  • macroautophagy Source: SGD
  • protein localization by the Cvt pathway Source: SGD
  • protein localization to phagophore assembly site Source: SGD
  • protein transport Source: UniProtKB-KW
  • vesicle organization Source: SGD

Keywordsi

Biological processAutophagy, Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31613-MONOMER

Protein family/group databases

TCDBi9.A.15.1.1 the autophagy-related phagophore-formation transporter (apt) family

Names & Taxonomyi

Protein namesi
Recommended name:
Autophagy-related protein 27
Alternative name(s):
Enhancer of VPS34 missorting protein 1
Gene namesi
Name:ATG27
Synonyms:ETF1
Ordered Locus Names:YJL178C
ORF Names:J0490
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJL178C
SGDiS000003714 ATG27

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini20 – 199Lumenal1 PublicationAdd BLAST180
Transmembranei200 – 220HelicalSequence analysisAdd BLAST21
Topological domaini221 – 271Cytoplasmic1 PublicationAdd BLAST51

Keywords - Cellular componenti

Cytoplasmic vesicle, Golgi apparatus, Membrane, Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi188 – 193KKPAKK → AAPAAA: Abolishes the binding of phosphatidylinositol 3-phosphate and the cytoplasm to vacuole transport. 1 Publication6

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000000178020 – 271Autophagy-related protein 27Add BLAST252

Proteomic databases

MaxQBiP46989
PaxDbiP46989
PRIDEiP46989
TopDownProteomicsiP46989

Interactioni

Subunit structurei

Forms a complex with ATG9 and ATG23.1 Publication

Protein-protein interaction databases

BioGridi33583, 163 interactors
DIPiDIP-1606N
IntActiP46989, 21 interactors
MINTiP46989
STRINGi4932.YJL178C

Structurei

3D structure databases

ProteinModelPortaliP46989
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATG27 family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000034146
InParanoidiP46989
KOiK21141
OMAiSWGWFTW
OrthoDBiEOG092C478W

Family and domain databases

Gene3Di2.70.130.10, 1 hit
InterProiView protein in InterPro
IPR018939 Autophagy-rel_prot_27
IPR009011 Man6P_isomerase_rcpt-bd_dom_sf
PfamiView protein in Pfam
PF09451 ATG27, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P46989-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSKTWICGF ISIITVVQAL SCEKHDVLKK YQVGKFSSLT STERDTPPST
60 70 80 90 100
TIEKWWINVC EEHNVEPPEE CKKNDMLCGL TDVILPGKDA ITTQIIDFDK
110 120 130 140 150
NIGFNVEETE SALTLTLKGA TWGANSFDAK LEFQCNDNMK QDELTSHTWA
160 170 180 190 200
DKSIQLTLKG PSGCLKSKDD DKKNGDGDNG KDGDSEGKKP AKKAGGTSWF
210 220 230 240 250
TWLFLYALLF TLIYLMVVSF LNTRGGSFQD FRAEFIQRST QFLTSLPEFC
260 270
KEVVSRILGR STAQRGGYSA V
Length:271
Mass (Da):30,201
Last modified:March 15, 2005 - v2
Checksum:iBD2F3BF4C5904ED2
GO

Sequence cautioni

The sequence AAS56577 differs from that shown. Reason: Frameshift at position 68.Curated
The sequence CAA89473 differs from that shown. Reason: Frameshift at position 68.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49453 Genomic DNA Translation: CAA89473.1 Frameshift.
AY558251 Genomic DNA Translation: AAS56577.1 Frameshift.
AY260894 Genomic DNA Translation: AAP21762.1
BK006943 Genomic DNA Translation: DAA08627.1
PIRiS56961
RefSeqiNP_012357.2, NM_001181611.1

Genome annotation databases

EnsemblFungiiYJL178C; YJL178C; YJL178C
GeneIDi853261
KEGGisce:YJL178C

Similar proteinsi

Entry informationi

Entry nameiATG27_YEAST
AccessioniPrimary (citable) accession number: P46989
Secondary accession number(s): D6VW11, Q86ZR9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: March 15, 2005
Last modified: March 28, 2018
This is version 129 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health