P46985 (MNN11_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable alpha-1,6-mannosyltransferase MNN11
EC=2.4.1.-

Alternative name(s):
Mannan polymerase II complex MNN11 subunit
Short name=M-Pol II subunit MNN11

Gene names

Name:	MNN11
Ordered Locus Names:	YJL183W
ORF Names:	J0425

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	422 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for synthesis of full length mannan chains. Ref.5 The M-Pol II complex possesses alpha-1,6-mannosyltransferase activity and is probably involved in the elongation of the mannan backbone of N-linked glycans on cell wall and periplasmic proteins. Ref.5
Subunit structure	Component of the M-Pol II complex composed of ANP1, MNN9, MNN10, MNN11 and HOC1. Ref.4 Ref.5
Subcellular location	Golgi apparatus › cis-Golgi network membrane; Single-pass type II membrane protein Ref.4 Ref.6.
Miscellaneous	Present with 3480 molecules/cell in log phase SD medium. Ref.7
Sequence similarities	Belongs to the glycosyltransferase 34 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	cell wall mannoprotein biosynthetic process Inferred from direct assay Ref.5. Source: UniProtKB protein glycosylation Inferred from mutant phenotype. Source: SGD
Cellular component	alpha-1,6-mannosyltransferase complex Inferred from direct assay Ref.5. Source: UniProtKB integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alpha-1,6-mannosyltransferase activity Inferred from direct assay Ref.4. Source: UniProtKB protein binding Inferred from physical interaction Ref.4. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ANP1	P32629	3	EBI-11052,EBI-2595
HOC1	P47124	2	EBI-11052,EBI-8430
MNN10	P50108	5	EBI-11052,EBI-11043

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 422	422	Probable alpha-1,6-mannosyltransferase MNN11		PRO_0000215165
Regions
	Topological domain	1 – 31	31	Cytoplasmic Potential
	Transmembrane	32 – 52	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	53 – 422	370	Lumenal Potential
Experimental info
	Sequence conflict	298	1	D → G in CAA89478. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P46985 [UniParc]. Last modified September 21, 2011. Version 2. Checksum: B1764ADBC19F9E58	FASTA	422	47,859
	10 20 30 40 50 60 MAIKPRTKGK TYSSRSVGSQ WFNRLGFKQN KYGTCKFLSI ITAFVFILYF FSNRFYPISR 70 80 90 100 110 120 SAGASYSPSH GLYINEIPAS SRLIYPHVEH VPVLKQMTVR GLYITRLEVD GSKRLILKPE 130 140 150 160 170 180 ENALTDEEKK KTTDQILLVK HSFLDHGKLV YRKSNDAPEV VVVTLIDFEN YELETIIQIV 190 200 210 220 230 240 QNRVDYAQKH QYGVYIRWIQ EFLPVLENQN LAESYEFIKP LVIRAAMHAF PTAKYIHFVD 250 260 270 280 290 300 QDALLMNLDL SLQKYLLDPK IMDLALLKNV PVVANSNIKT YNHFEYSSAK IIIPHDADGN 310 320 330 340 350 360 IDASSFVIAN DFYGKALIDY LNDPLLRNFP WDNTGDKLSA AIGHILQWHP TLLGKTAIVI 370 380 390 400 410 420 PKVLASQYDA SLDQEGESGN GASNGDVYHY NEGDLAASFK GCRSRGTCAS EIGHMYQKIK KS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 298. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Multi-protein complexes in the cis Golgi of Saccharomyces cerevisiae with alpha-1,6-mannosyltransferase activity." Jungmann J., Munro S. EMBO J. 17:423-434(1998) [PubMed: 9430634] [Abstract] Cited for: PROTEIN SEQUENCE OF N-TERMINUS, SUBUNIT, SUBCELLULAR LOCATION, ACTIVITY OF M-POL II COMPLEX.
[5]	"The Saccharomyces cerevisiae protein Mnn10p/Bed1p is a subunit of a Golgi mannosyltransferase complex." Jungmann J., Rayner J.C., Munro S. J. Biol. Chem. 274:6579-6585(1999) [PubMed: 10037752] [Abstract] Cited for: FUNCTION, SUBUNIT.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z49458 Genomic DNA. Translation: CAA89478.1. AY692909 Genomic DNA. Translation: AAT92928.1. BK006943 Genomic DNA. Translation: DAA08623.2.
PIR	S56966.
RefSeq	NP_012352.2. NM_001181616.2.
3D structure databases
ProteinModelPortal	P46985.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-914N.
IntAct	P46985. 22 interactions.
STRING	P46985.
Protein family/group databases
CAZy	GT34. Glycosyltransferase Family 34.
Proteomic databases
PeptideAtlas	P46985.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	853256.
KEGG	sce:YJL183W.
NMPDR	fig|4932.3.peg.3316.
Organism-specific databases
CYGD	YJL183w.
SGD	S000003719. MNN11.
Phylogenomic databases
eggNOG	fuNOG08674.
HOGENOM	HBG202276.
OMA	PDSALTH.
OrthoDB	EOG46T69N.
Gene expression databases
ArrayExpress	P46985.
Genevestigator	P46985.
GermOnline	YJL183W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR008630. Glyco_trans_34. [Graphical view]
KO	K05532.
Pfam	PF05637. Glyco_transf_34. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	973508.

Entry information

Entry name

MNN11_YEAST

Accession

Primary (citable) accession number: P46985
Secondary accession number(s): D6VW07, E9P8Z0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	September 21, 2011
Last modified:	January 25, 2012
This is version 88 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program