P46982 (MNN5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-1,2-mannosyltransferase MNN5
EC=2.4.1.-

Gene names

Name:	MNN5
Ordered Locus Names:	YJL186W
ORF Names:	J0409

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	586 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Responsible for addition of first and second mannose residues to the outer chain of core N-linked polysaccharides and to O-linked mannotriose. Implicated in late Golgi modifications. Ref.3 Ref.4 Ref.5
Pathway	Protein modification; protein glycosylation.
Subunit structure	Interacts with SVP26. Ref.7
Subcellular location	Golgi apparatus › cis-Golgi network. Note: Golgi localization depends on SVP26. Ref.5 Ref.6 Ref.7
Post-translational modification	Glycosylated. Ref.5
Miscellaneous	Present with 11400 molecules/cell in log phase SD medium. Ref.8
Sequence similarities	Belongs to the MNN1/MNT family.

Ontologies

Keywords
Cellular component	Golgi apparatus
Domain	Signal
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein glycosylation Inferred from mutant phenotype Ref.5. Source: SGD
Cellular component	Golgi apparatus Inferred from direct assay Ref.5. Source: SGD
Molecular function	alpha-1,2-mannosyltransferase activity Inferred from mutant phenotype Ref.5. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29	Potential
	Chain	30 – 586	557	Alpha-1,2-mannosyltransferase MNN5		PRO_0000203019
Amino acid modifications
	Glycosylation	113	1	N-linked (GlcNAc...) Potential
	Glycosylation	136	1	N-linked (GlcNAc...) Potential
	Glycosylation	259	1	N-linked (GlcNAc...) Potential
	Glycosylation	264	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	395	1	V → F in CAA89481. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P46982 [UniParc]. Last modified September 21, 2011. Version 2. Checksum: 4AE911B415E354E6	FASTA	586	67,204
	10 20 30 40 50 60 MLIRLKKRKI LQVIVSAVVL ILFFCSVHND VSSSWLYGKK LRLPVLTRSN LKNNFYTTLV 70 80 90 100 110 120 QAIVENKPAD SSPDLSKLHG AEGCSFANNV AAHDSGHDSD LSYESLSKCY NLNKTVQESL 130 140 150 160 170 180 REVHSKFTDT LSGKLNFSIP QREALFSGSE GIVTIGGGKY SVLAYTMIKK LRDTGTTLPI 190 200 210 220 230 240 EVIIPPQDEG EDDFCKNWLP KFNGKCIYFS DIVPSKPLSD LKLTHFQLKV FGLIISSFKR 250 260 270 280 290 300 IIFLDADNYA VKNLDLAFNT TSFNDTGLIL WPDFWRRVTP PAFYNIIGSS INIGKRVRFV 310 320 330 340 350 360 SDDISPVSRY DPFVSNSNDY TPKERQEHFL KHVPLHDLDG TMPDLSSESG QMVIDKIRHF 370 380 390 400 410 420 NTLLLALYYN VYGPTWYYKM ISQGTAGEGD KDTFVAAAHA LNMPYYQVRT NFEFDGFFYQ 430 440 450 460 470 480 KDDYKGLALL QHDFEQDYKQ YQKAQQKVKA NIEEFSKLDP DYTLDNGFLK TLMVNDDGSD 490 500 510 520 530 540 LDIMFIHASF YKADPWTLYH ENRFIGPNGE QVRGFRKPHR YGMDFELFLF NDMRGSFCTT 550 560 570 580 PKSQVIKFKY FTDKVNTPEW DAMCEYLTNH VNYLESTHKE AMGEKN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 395. Strain: ATCC 204508 / S288c.
[3]	"Saccharomyces cerevisiae mannoprotein mutants. Isolation of the mnn5 mutant and comparison with the mnn3 strain." Cohen R.E., Ballou L., Ballou C.E. J. Biol. Chem. 255:7700-7707(1980) [PubMed: 6995454] [Abstract] Cited for: FUNCTION.
[4]	"Effects of mannoprotein mutations on Saccharomyces cerevisiae core oligosaccharide structure." Cohen R.E., Zhang W., Ballou C.E. J. Biol. Chem. 257:5730-5737(1982) [PubMed: 6802821] [Abstract] Cited for: FUNCTION.
[5]	"Identification of the MNN2 and MNN5 mannosyltransferases required for forming and extending the mannose branches of the outer chain mannans of Saccharomyces cerevisiae." Rayner J.C., Munro S. J. Biol. Chem. 273:26836-26843(1998) [PubMed: 9756928] [Abstract] Cited for: FUNCTION, GLYCOSYLATION, SUBCELLULAR LOCATION.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Svp26 facilitates endoplasmic reticulum to Golgi transport of a set of mannosyltransferases in Saccharomyces cerevisiae." Noda Y., Yoda K. J. Biol. Chem. 285:15420-15429(2010) [PubMed: 20236934] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH SVP26.
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z49461 Genomic DNA. Translation: CAA89481.1. BK006943 Genomic DNA. Translation: DAA08620.2.
PIR	S56969.
RefSeq	NP_012349.2. NM_001181619.2.
3D structure databases
ProteinModelPortal	P46982.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2868N.
IntAct	P46982. 1 interaction.
STRING	P46982.
Protein family/group databases
CAZy	GT71. Glycosyltransferase Family 71.
Proteomic databases
PeptideAtlas	P46982.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	853253.
KEGG	sce:YJL186W.
NMPDR	fig|4932.3.peg.3313.
Organism-specific databases
CYGD	YJL186w.
SGD	S000003722. MNN5.
Phylogenomic databases
eggNOG	fuNOG05581.
GeneTree	EFGT00050000003822.
HOGENOM	HBG204123.
OMA	PYYQVKT.
OrthoDB	EOG4R273T.
Gene expression databases
ArrayExpress	P46982.
Genevestigator	P46982.
GermOnline	YJL186W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR022751. Alpha_mannosyltransferase. [Graphical view]
KO	K05536.
Pfam	PF11051. Mannosyl_trans3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	973499.

Entry information

Entry name

MNN5_YEAST

Accession

Primary (citable) accession number: P46982
Secondary accession number(s): D6VW04

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	September 21, 2011
Last modified:	December 14, 2011
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program