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Protein

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A

Gene

Stt3a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). SST3A seems to be involved in complex substrate specificity. STT3A is present in the majority of OST complexes and mediates cotranslational N-glycosylation of most sites on target proteins, while STT3B-containing complexes are required for efficient cotranslational glycosylation and mediate glycosylation of sites that have been skipped by STT3A (By similarity).By similarity

Catalytic activityi

Dolichyl diphosphooligosaccharide + [protein]-L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT66. Glycosyltransferase Family 66.

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A (EC:2.4.99.18)
Short name:
Oligosaccharyl transferase subunit STT3A
Short name:
STT3-A
Alternative name(s):
B5
Integral membrane protein 1
Gene namesi
Name:Stt3a
Synonyms:Itm1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:105124. Stt3a.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1717CytoplasmicSequence analysisAdd
BLAST
Transmembranei18 – 3821HelicalSequence analysisAdd
BLAST
Topological domaini39 – 11476LumenalSequence analysisAdd
BLAST
Transmembranei115 – 13521HelicalSequence analysisAdd
BLAST
Topological domaini136 – 16934CytoplasmicSequence analysisAdd
BLAST
Transmembranei170 – 19021HelicalSequence analysisAdd
BLAST
Topological domaini191 – 20818LumenalSequence analysisAdd
BLAST
Transmembranei209 – 22921HelicalSequence analysisAdd
BLAST
Topological domaini230 – 2356CytoplasmicSequence analysis
Transmembranei236 – 25924HelicalSequence analysisAdd
BLAST
Topological domaini260 – 2656LumenalSequence analysis
Transmembranei266 – 28621HelicalSequence analysisAdd
BLAST
Topological domaini287 – 30014CytoplasmicSequence analysisAdd
BLAST
Transmembranei301 – 32121HelicalSequence analysisAdd
BLAST
Topological domaini322 – 35938LumenalSequence analysisAdd
BLAST
Transmembranei360 – 38021HelicalSequence analysisAdd
BLAST
Topological domaini381 – 3833CytoplasmicSequence analysis
Transmembranei384 – 40421HelicalSequence analysisAdd
BLAST
Topological domaini405 – 4051LumenalSequence analysis
Transmembranei406 – 42621HelicalSequence analysisAdd
BLAST
Topological domaini427 – 45327CytoplasmicSequence analysisAdd
BLAST
Transmembranei454 – 47421HelicalSequence analysisAdd
BLAST
Topological domaini475 – 705231LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: MGI
  • oligosaccharyltransferase complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 705705Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3APRO_0000072291Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi537 – 5371N-linked (GlcNAc...)Sequence analysis
Glycosylationi544 – 5441N-linked (GlcNAc...)Sequence analysis
Glycosylationi548 – 5481N-linked (GlcNAc...)1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiP46978.
PaxDbiP46978.
PeptideAtlasiP46978.
PRIDEiP46978.

PTM databases

iPTMnetiP46978.
PhosphoSiteiP46978.
SwissPalmiP46978.

Expressioni

Gene expression databases

BgeeiP46978.
ExpressionAtlasiP46978. baseline and differential.
GenevisibleiP46978. MM.

Interactioni

Subunit structurei

Component of the oligosaccharyltransferase (OST) complex. OST seems to exist in different forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes. Also identified as part of a complex which includes CANX, DERL1, DERL2, DDOST/OST48, RPN1, RPN2, SELK, VIMP, STT3A AND VCP. This contains known members of the OST complex and may be a form of this complex (By similarity).By similarity

Protein-protein interaction databases

IntActiP46978. 2 interactions.
MINTiMINT-4135975.
STRINGi10090.ENSMUSP00000113116.

Structurei

3D structure databases

ProteinModelPortaliP46978.
SMRiP46978. Positions 20-183, 474-701.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the STT3 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2292. Eukaryota.
COG1287. LUCA.
HOGENOMiHOG000157471.
HOVERGENiHBG010606.
InParanoidiP46978.
KOiK07151.
OMAiPIDVKMT.
OrthoDBiEOG7VHSWP.
PhylomeDBiP46978.
TreeFamiTF300822.

Family and domain databases

InterProiIPR003674. Oligo_trans_STT3.
[Graphical view]
PfamiPF02516. STT3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P46978-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKLGFLRLS YEKQDTLLKL LILSMAAVLS FSTRLFAVLR FESVIHEFDP
60 70 80 90 100
YFNYRTTRFL AEEGFYKFHN WFDDRAWYPL GRIIGGTIYP GLMITSAAIY
110 120 130 140 150
HVLHFFHITI DIRNVCVFLA PLFSSFTTIV TYHLTKELKD AGAGLLAAAM
160 170 180 190 200
IAVVPGYISR SVAGSYDNEG IAIFCMLLTY YMWIKAVKTG SIYWAAKCAL
210 220 230 240 250
AYFYMVSSWG GYVFLINLIP LHVLVLMLTG RFSHRIYVAY CTVYCLGTIL
260 270 280 290 300
SMQISFVGFQ PVLSSEHMAA FGVFGLCQIH AFVDYLRSKL NPQQFEVLFR
310 320 330 340 350
SVISLVGFVL LTVGALLMLT GKISPWTGRF YSLLDPSYAK NNIPIIASVS
360 370 380 390 400
EHQPTTWSSY YFDLQLLVFM FPVGLYYCFS NLSDARIFII MYGVTSMYFS
410 420 430 440 450
AVMVRLMLVL APVMCILSGI GVSQVLSTYM KNLDISRPDK KSKKQQDSTY
460 470 480 490 500
PIKNEVASGM ILVMAFFLIT YTFHSTWVTS EAYSSPSIVL SARGGDGSRI
510 520 530 540 550
IFDDFREAYY WLRHNTPEDA KVMSWWDYGY QITAMANRTI LVDNNTWNNT
560 570 580 590 600
HISRVGQAMA STEEKAYEIM RELDVSYVLV IFGGLTGYSS DDINKFLWMV
610 620 630 640 650
RIGGSTETGR HIKENDYYTP TGEFRVDREG SPVLLNCLMY KMCYYRFGQV
660 670 680 690 700
YTEAKRPPGF DRVRNAEIGN KDFELDVLEE AYTTEHWLVR IYKVKDLDNR

GLSRT
Length:705
Mass (Da):80,598
Last modified:November 1, 1995 - v1
Checksum:iCD1D796D0479F321
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L34260 mRNA. Translation: AAB47775.1.
BC037612 mRNA. Translation: AAH37612.1.
CCDSiCCDS52768.1.
RefSeqiNP_032434.3. NM_008408.4.
UniGeneiMm.2863.

Genome annotation databases

EnsembliENSMUST00000120381; ENSMUSP00000113116; ENSMUSG00000032116.
GeneIDi16430.
KEGGimmu:16430.
UCSCiuc009oty.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L34260 mRNA. Translation: AAB47775.1.
BC037612 mRNA. Translation: AAH37612.1.
CCDSiCCDS52768.1.
RefSeqiNP_032434.3. NM_008408.4.
UniGeneiMm.2863.

3D structure databases

ProteinModelPortaliP46978.
SMRiP46978. Positions 20-183, 474-701.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP46978. 2 interactions.
MINTiMINT-4135975.
STRINGi10090.ENSMUSP00000113116.

Protein family/group databases

CAZyiGT66. Glycosyltransferase Family 66.

PTM databases

iPTMnetiP46978.
PhosphoSiteiP46978.
SwissPalmiP46978.

Proteomic databases

EPDiP46978.
PaxDbiP46978.
PeptideAtlasiP46978.
PRIDEiP46978.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000120381; ENSMUSP00000113116; ENSMUSG00000032116.
GeneIDi16430.
KEGGimmu:16430.
UCSCiuc009oty.1. mouse.

Organism-specific databases

CTDi3703.
MGIiMGI:105124. Stt3a.

Phylogenomic databases

eggNOGiKOG2292. Eukaryota.
COG1287. LUCA.
HOGENOMiHOG000157471.
HOVERGENiHBG010606.
InParanoidiP46978.
KOiK07151.
OMAiPIDVKMT.
OrthoDBiEOG7VHSWP.
PhylomeDBiP46978.
TreeFamiTF300822.

Enzyme and pathway databases

UniPathwayiUPA00378.

Miscellaneous databases

ChiTaRSiStt3a. mouse.
PROiP46978.
SOURCEiSearch...

Gene expression databases

BgeeiP46978.
ExpressionAtlasiP46978. baseline and differential.
GenevisibleiP46978. MM.

Family and domain databases

InterProiIPR003674. Oligo_trans_STT3.
[Graphical view]
PfamiPF02516. STT3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of a highly conserved mouse and human integral membrane protein (Itm1) and genetic mapping to mouse chromosome 9."
    Hong G., Deleersnjider W., Kozak C.A., van Marck E., Tylzanowski P., Merregaert J.
    Genomics 31:295-300(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/cJ.
    Tissue: Mandibular condyle.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary gland.
  3. Lubec G., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 696-704, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: OF1.
    Tissue: Hippocampus.
  4. "Oligosaccharyltransferase isoforms that contain different catalytic STT3 subunits have distinct enzymatic properties."
    Kelleher D.J., Karaoglu D., Mandon E.C., Gilmore R.
    Mol. Cell 12:101-111(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  5. "Membrane topology of the STT3 subunit of the oligosaccharyl transferase complex."
    Kim H., von Heijne G., Nilsson I.
    J. Biol. Chem. 280:20261-20267(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY.
  6. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
    Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
    Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-548.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brown adipose tissue, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiSTT3A_MOUSE
AccessioniPrimary (citable) accession number: P46978
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 6, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.