UniProtKB - P46977 (STT3A_HUMAN)
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Protein
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A
Gene
STT3A
Organism
Homo sapiens (Human)
Status
Functioni
Catalytic subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). SST3A seems to be involved in complex substrate specificity. STT3A is present in the majority of OST complexes and mediates cotranslational N-glycosylation of most sites on target proteins, while STT3B-containing complexes are required for efficient post-translational glycosylation and mediate glycosylation of sites that have been skipped by STT3A.By similarityCurated1 Publication
Catalytic activityi
Dolichyl diphosphooligosaccharide + [protein]-L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
GO - Molecular functioni
- dolichyl-diphosphooligosaccharide-protein glycotransferase activity Source: UniProtKB
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- co-translational protein modification Source: UniProtKB
- post-translational protein modification Source: GO_Central
- protein N-linked glycosylation via asparagine Source: UniProtKB
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
Reactomei | R-HSA-446203 Asparagine N-linked glycosylation |
SignaLinki | P46977 |
UniPathwayi | UPA00378 |
Protein family/group databases
CAZyi | GT66 Glycosyltransferase Family 66 |
TCDBi | 9.B.142.3.4 the integral membrane glycosyltransferase family 39 (gt39) family |
Names & Taxonomyi
Protein namesi | Recommended name: Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A (EC:2.4.99.18)Short name: Oligosaccharyl transferase subunit STT3A Short name: STT3-A Alternative name(s): B5 Integral membrane protein 1 Transmembrane protein TMC |
Gene namesi | Name:STT3A Synonyms:ITM1, TMC |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000134910.12 |
HGNCi | HGNC:6172 STT3A |
MIMi | 601134 gene |
neXtProti | NX_P46977 |
Subcellular locationi
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 17 | CytoplasmicSequence analysisAdd BLAST | 17 | |
Transmembranei | 18 – 38 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 39 – 114 | LumenalSequence analysisAdd BLAST | 76 | |
Transmembranei | 115 – 135 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 136 – 169 | CytoplasmicSequence analysisAdd BLAST | 34 | |
Transmembranei | 170 – 190 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 191 – 208 | LumenalSequence analysisAdd BLAST | 18 | |
Transmembranei | 209 – 229 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 230 – 235 | CytoplasmicSequence analysis | 6 | |
Transmembranei | 236 – 259 | HelicalSequence analysisAdd BLAST | 24 | |
Topological domaini | 260 – 265 | LumenalSequence analysis | 6 | |
Transmembranei | 266 – 286 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 287 – 300 | CytoplasmicSequence analysisAdd BLAST | 14 | |
Transmembranei | 301 – 321 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 322 – 359 | LumenalSequence analysisAdd BLAST | 38 | |
Transmembranei | 360 – 380 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 381 – 383 | CytoplasmicSequence analysis | 3 | |
Transmembranei | 384 – 404 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 405 | LumenalSequence analysis | 1 | |
Transmembranei | 406 – 426 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 427 – 453 | CytoplasmicSequence analysisAdd BLAST | 27 | |
Transmembranei | 454 – 474 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 475 – 705 | LumenalSequence analysisAdd BLAST | 231 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePathology & Biotechi
Involvement in diseasei
Congenital disorder of glycosylation 1W (CDG1W)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA form of congenital disorder of glycosylation, a multisystem disorder caused by a defect in glycoprotein biosynthesis and characterized by under-glycosylated serum glycoproteins. Congenital disorders of glycosylation result in a wide variety of clinical features, such as defects in the nervous system development, psychomotor retardation, dysmorphic features, hypotonia, coagulation disorders, and immunodeficiency. The broad spectrum of features reflects the critical role of N-glycoproteins during embryonic development, differentiation, and maintenance of cell functions.
See also OMIM:615596Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_070944 | 626 | V → A in CDG1W; affects activity resulting in hypoglycosylation of STT3A-specific substrates. 1 PublicationCorresponds to variant dbSNP:rs587777216Ensembl. | 1 |
Keywords - Diseasei
Congenital disorder of glycosylation, Disease mutationOrganism-specific databases
DisGeNETi | 3703 |
MalaCardsi | STT3A |
MIMi | 615596 phenotype |
OpenTargetsi | ENSG00000134910 |
Orphaneti | 370921 STT3A-CDG |
PharmGKBi | PA29969 |
Polymorphism and mutation databases
DMDMi | 182676409 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000072290 | 1 – 705 | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3AAdd BLAST | 705 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 537 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 544 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 548 | N-linked (GlcNAc...) asparagine1 Publication | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
EPDi | P46977 |
MaxQBi | P46977 |
PaxDbi | P46977 |
PeptideAtlasi | P46977 |
PRIDEi | P46977 |
PTM databases
iPTMneti | P46977 |
PhosphoSitePlusi | P46977 |
SwissPalmi | P46977 |
Expressioni
Tissue specificityi
Expressed at high levels in placenta, liver, muscle and pancreas, and at very low levels in brain, lung and kidney. Expressed in skin fibroblasts (at protein level).1 Publication
Gene expression databases
Bgeei | ENSG00000134910 |
CleanExi | HS_STT3A |
ExpressionAtlasi | P46977 baseline and differential |
Genevisiblei | P46977 HS |
Organism-specific databases
HPAi | HPA030735 |
Interactioni
Subunit structurei
Component of the oligosaccharyltransferase (OST) complex. OST exists in two different complex forms which contain common core subunits RPN1, RPN2, OST48, OST4, DAD1 and TMEM258, either STT3A or STT3B as catalytic subunits, and form-specific accessory subunits. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes.By similarityCurated2 Publications
Binary interactionsi
Protein-protein interaction databases
BioGridi | 10990825 interactors. |
CORUMi | P46977 |
IntActi | P46977 40 interactors. |
MINTi | P46977 |
STRINGi | 9606.ENSP00000376472 |
Family & Domainsi
Sequence similaritiesi
Belongs to the STT3 family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2292 Eukaryota COG1287 LUCA |
GeneTreei | ENSGT00390000015238 |
HOGENOMi | HOG000157471 |
HOVERGENi | HBG010606 |
InParanoidi | P46977 |
KOi | K07151 |
OMAi | SWSSFYF |
OrthoDBi | EOG091G02DB |
PhylomeDBi | P46977 |
TreeFami | TF300822 |
Family and domain databases
InterProi | View protein in InterPro IPR003674 Oligo_trans_STT3 |
Pfami | View protein in Pfam PF02516 STT3, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basket
Isoform 1 (identifier: P46977-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
10 20 30 40 50
MTKFGFLRLS YEKQDTLLKL LILSMAAVLS FSTRLFAVLR FESVIHEFDP
60 70 80 90 100
YFNYRTTRFL AEEGFYKFHN WFDDRAWYPL GRIIGGTIYP GLMITSAAIY
110 120 130 140 150
HVLHFFHITI DIRNVCVFLA PLFSSFTTIV TYHLTKELKD AGAGLLAAAM
160 170 180 190 200
IAVVPGYISR SVAGSYDNEG IAIFCMLLTY YMWIKAVKTG SICWAAKCAL
210 220 230 240 250
AYFYMVSSWG GYVFLINLIP LHVLVLMLTG RFSHRIYVAY CTVYCLGTIL
260 270 280 290 300
SMQISFVGFQ PVLSSEHMAA FGVFGLCQIH AFVDYLRSKL NPQQFEVLFR
310 320 330 340 350
SVISLVGFVL LTVGALLMLT GKISPWTGRF YSLLDPSYAK NNIPIIASVS
360 370 380 390 400
EHQPTTWSSY YFDLQLLVFM FPVGLYYCFS NLSDARIFII MYGVTSMYFS
410 420 430 440 450
AVMVRLMLVL APVMCILSGI GVSQVLSTYM KNLDISRPDK KSKKQQDSTY
460 470 480 490 500
PIKNEVASGM ILVMAFFLIT YTFHSTWVTS EAYSSPSIVL SARGGDGSRI
510 520 530 540 550
IFDDFREAYY WLRHNTPEDA KVMSWWDYGY QITAMANRTI LVDNNTWNNT
560 570 580 590 600
HISRVGQAMA STEEKAYEIM RELDVSYVLV IFGGLTGYSS DDINKFLWMV
610 620 630 640 650
RIGGSTDTGK HIKENDYYTP TGEFRVDREG SPVLLNCLMY KMCYYRFGQV
660 670 680 690 700
YTEAKRPPGF DRVRNAEIGN KDFELDVLEE AYTTEHWLVR IYKVKDLDNR
GLSRT
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 61 | A → S in AAL77539 (PubMed:8634329).Curated | 1 | |
Sequence conflicti | 117 | V → M in BAG58686 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 128 | T → S in AAB05994 (PubMed:8838310).Curated | 1 | |
Sequence conflicti | 133 | H → L in AAB05994 (PubMed:8838310).Curated | 1 | |
Sequence conflicti | 252 | M → R in AAB05994 (PubMed:8838310).Curated | 1 | |
Sequence conflicti | 270 | A → G in AAB05994 (PubMed:8838310).Curated | 1 | |
Sequence conflicti | 415 | C → S in AAB05994 (PubMed:8838310).Curated | 1 | |
Sequence conflicti | 454 | N → I in AAB05994 (PubMed:8838310).Curated | 1 | |
Sequence conflicti | 494 | G → D in AAL77539 (PubMed:8634329).Curated | 1 | |
Sequence conflicti | 681 | A → G in AAB05994 (PubMed:8838310).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_070944 | 626 | V → A in CDG1W; affects activity resulting in hypoglycosylation of STT3A-specific substrates. 1 PublicationCorresponds to variant dbSNP:rs587777216Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_055106 | 1 – 92 | Missing in isoform 2. 1 PublicationAdd BLAST | 92 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L38961 mRNA Translation: AAB05994.1 L47337 mRNA Translation: AAL77539.1 AK290040 mRNA Translation: BAF82729.1 AK290657 mRNA Translation: BAF83346.1 AK295892 mRNA Translation: BAG58686.1 BT007100 mRNA Translation: AAP35764.1 AP001132 Genomic DNA No translation available. AP001494 Genomic DNA No translation available. CH471065 Genomic DNA Translation: EAW67647.1 BC020965 mRNA Translation: AAH20965.1 BC048348 mRNA Translation: AAH48348.2 |
CCDSi | CCDS60998.1 [P46977-2] CCDS8458.1 [P46977-1] |
PIRi | S70029 |
RefSeqi | NP_001265432.1, NM_001278503.1 [P46977-1] NP_001265433.1, NM_001278504.1 [P46977-2] NP_689926.1, NM_152713.4 [P46977-1] XP_011541109.1, XM_011542807.2 [P46977-1] |
UniGenei | Hs.504237 Hs.659244 |
Genome annotation databases
Ensembli | ENST00000392708; ENSP00000376472; ENSG00000134910 [P46977-1] ENST00000529196; ENSP00000436962; ENSG00000134910 [P46977-1] ENST00000531491; ENSP00000432820; ENSG00000134910 [P46977-2] |
GeneIDi | 3703 |
KEGGi | hsa:3703 |
UCSCi | uc001qcd.4 human [P46977-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Entry informationi
Entry namei | STT3A_HUMAN | |
Accessioni | P46977Primary (citable) accession number: P46977 Secondary accession number(s): B4DJ24 Q8WUB4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | April 8, 2008 | |
Last modified: | March 28, 2018 | |
This is version 151 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |