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P46975 (STT3_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3
Short name=Oligosaccharyl transferase subunit STT3
Short name=STT3
EC=2.4.99.18
Gene names
ORF Names:T12A2.2
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length757 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in protein glycosylation. Either required for the assembly of the oligosaccharyl transferase (OTase) complex or required in substoichiometric amounts for OTase activity By similarity.

Catalytic activity

Dolichyl diphosphooligosaccharide + [protein]-L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.

Pathway

Protein modification; protein glycosylation.

Subunit structure

Component of the oligosaccharyltransferase (OST) complex By similarity.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.

Sequence similarities

Belongs to the STT3 family.

Ontologies

Keywords
   Cellular componentEndoplasmic reticulum
Membrane
   DomainTransmembrane
Transmembrane helix
   Molecular functionGlycosyltransferase
Transferase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processprotein glycosylation

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentendoplasmic reticulum membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionoligosaccharyl transferase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 757757Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3
PRO_0000072289

Regions

Topological domain1 – 1313Cytoplasmic Potential
Transmembrane14 – 3421Helical; Potential
Topological domain35 – 11682Lumenal Potential
Transmembrane117 – 13721Helical; Potential
Topological domain138 – 17134Cytoplasmic Potential
Transmembrane172 – 19221Helical; Potential
Topological domain193 – 21018Lumenal Potential
Transmembrane211 – 23121Helical; Potential
Topological domain232 – 2387Cytoplasmic Potential
Transmembrane239 – 25921Helical; Potential
Topological domain260 – 2678Lumenal Potential
Transmembrane268 – 28821Helical; Potential
Topological domain289 – 29911Cytoplasmic Potential
Transmembrane300 – 32021Helical; Potential
Topological domain321 – 35838Lumenal Potential
Transmembrane359 – 37921Helical; Potential
Topological domain380 – 3889Cytoplasmic Potential
Transmembrane389 – 40921Helical; Potential
Topological domain410 – 4112Lumenal Potential
Transmembrane412 – 43221Helical; Potential
Topological domain433 – 47947Cytoplasmic Potential
Transmembrane480 – 50021Helical; Potential
Topological domain501 – 757257Lumenal Potential

Amino acid modifications

Glycosylation5591N-linked (GlcNAc...) Ref.2 Ref.3
Glycosylation5661N-linked (GlcNAc...) Potential
Glycosylation5701N-linked (GlcNAc...) Ref.2 Ref.3
Glycosylation5841N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P46975 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: A6BF0BB019B6C4FF

FASTA75785,122
        10         20         30         40         50         60 
MTSTTAARTA SSRVGATTLL TIVVLALAWF VGFASRLFAI VRFESIIHEF DPWFNYRATH 

        70         80         90        100        110        120 
HMVQHGFYKF LNWFDERAWY PLGRIVGGTV YPGLMVTSGL IHWILDSLNF HVHIREVCVF 

       130        140        150        160        170        180 
LAPTFSGLTA IATYLLTKEL WSPGAGLFAA CFIAISPGYT SRSVAGSYDN EGIAIFALQF 

       190        200        210        220        230        240 
TYYLWVKSLK TGSIMWASLC ALSYFYMVSA WGGYVFIINL IPLHALALII MGRYSSRLFV 

       250        260        270        280        290        300 
SYTSFYCLAT ILSMQVPFVG FQPVRTSEHM PAFGVFGLLQ IVALMHYARN RITRQQFMTL 

       310        320        330        340        350        360 
FVGGLTILGA LSVVVYFALV WGGYVAPFSG RFYSLWDTGY AKIHIPIIAS VSEHQPTTWV 

       370        380        390        400        410        420 
SFFFDLHITA AVFPVGLWYC IKKVNDERVF IILYAVSAVY FAGVMVRLML TLTPAVCVLA 

       430        440        450        460        470        480 
GIGFSYTFEK YLKDEETKER SSSQSGTTKD EKLYDKAAKN VKSRNANDGD ESGVSSNVRT 

       490        500        510        520        530        540 
IISIILVIFL LMFVVHATYV TSNAYSHPSV VLQSSTNNGD RIIMDDFREA YHWLRENTAD 

       550        560        570        580        590        600 
DARVMSWWDY GYQIAGMANR TTLVDNNTWN NSHIALVGKA MSSNESAAYE IMTELDVDYI 

       610        620        630        640        650        660 
LVIFGGVIGY SGDDINKFLW MVRIAQGEHP KDIREENYFT STGEYSTGAG ASETMLNCLM 

       670        680        690        700        710        720 
YKMSYYRFGE TRVGYNQAGG FDRTRGYVIG KKDITLEYIE EAYTTENWLV RIYKRKKLPN 

       730        740        750 
RPTVKSEEAT IPIKGKKATQ GKNKKGVIRP APTASKA

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References

[1]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
[2]"Identification of the hydrophobic glycoproteins of Caenorhabditis elegans."
Fan X., She Y.-M., Bagshaw R.D., Callahan J.W., Schachter H., Mahuran D.J.
Glycobiology 15:952-964(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-559 AND ASN-570, MASS SPECTROMETRY.
[3]"Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins."
Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T.
Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-559 AND ASN-570, MASS SPECTROMETRY.
Strain: Bristol N2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		FO080619 Genomic DNA. Translation: CCD65209.1.
PIRT34351.
RefSeqNP_498362.1. NM_065961.3.

3D structure databases

ProteinModelPortalP46975.
SMRP46975. Positions 527-599.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-24951N.
MINTMINT-1069505.
STRING6239.T12A2.2.1.

Protein family/group databases

CAZyGT66. Glycosyltransferase Family 66.

Proteomic databases

PaxDbP46975.
PRIDEP46975.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaT12A2.2.1; T12A2.2.1; T12A2.2.
T12A2.2.2; T12A2.2.2; T12A2.2.
T12A2.2.3; T12A2.2.3; T12A2.2.
GeneID175886.
KEGGcel:CELE_T12A2.2.
UCSCT12A2.2.2. c. elegans.

Organism-specific databases

CTD175886.
WormBaseT12A2.2; CE01395; WBGene00020437.

Phylogenomic databases

eggNOGCOG1287.
GeneTreeENSGT00390000015238.
HOGENOMHOG000157471.
InParanoidP46975.
KOK07151.
OMAMWASLCA.

Enzyme and pathway databases

UniPathwayUPA00378.

Family and domain databases

InterProIPR003674. Oligo_trans_STT3.
[Graphical view]
PfamPF02516. STT3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio890152.

Entry information

Entry nameSTT3_CAEEL
AccessionPrimary (citable) accession number: P46975
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 1, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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