ID   HIT1_YEAST              Reviewed;         164 AA.
AC   P46973; D6VWM6;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   24-JAN-2024, entry version 148.
DE   RecName: Full=Protein HIT1;
GN   Name=HIT1; OrderedLocusNames=YJR055W; ORFNames=J1705;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1325386; DOI=10.1093/genetics/131.4.821;
RA   Kawakami K., Shafer B.K., Garfinkel D.J., Strathern J.N., Nakamura Y.;
RT   "Ty element-induced temperature-sensitive mutations of Saccharomyces
RT   cerevisiae.";
RL   Genetics 131:821-832(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=8840504;
RX   DOI=10.1002/(sici)1097-0061(199607)12:9<869::aid-yea964>3.0.co;2-1;
RA   Huang M.-E., Manus V., Chuat J.-C., Galibert F.;
RT   "Analysis of a 62 kb DNA sequence of chromosome X reveals 36 open reading
RT   frames and a gene cluster with a counterpart on chromosome XI.";
RL   Yeast 12:869-875(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=8641269; DOI=10.1002/j.1460-2075.1996.tb00557.x;
RA   Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C.,
RA   Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D.,
RA   Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J.,
RA   Heumann K., Hilger F., Hollenberg C.P., Huang M.-E., Jacq C.,
RA   Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E.,
RA   Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T.,
RA   Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B.,
RA   Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R.,
RA   Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N.,
RA   To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H.,
RA   von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.;
RT   "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.";
RL   EMBO J. 15:2031-2049(1996).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [6]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
CC   -!- INTERACTION:
CC       P46973; Q08932: RSA1; NbExp=8; IntAct=EBI-8359, EBI-16186;
CC   -!- MISCELLANEOUS: Present with 2730 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; D11103; BAA01878.1; -; Genomic_DNA.
DR   EMBL; Z49555; CAA89583.1; -; Genomic_DNA.
DR   EMBL; L47993; AAB39281.1; -; Genomic_DNA.
DR   EMBL; AY558097; AAS56423.1; -; Genomic_DNA.
DR   EMBL; BK006943; DAA08842.1; -; Genomic_DNA.
DR   PIR; S57074; S57074.
DR   RefSeq; NP_012589.1; NM_001181713.1.
DR   PDB; 2MJF; NMR; -; B=70-164.
DR   PDB; 2N95; NMR; -; A=1-46.
DR   PDBsum; 2MJF; -.
DR   PDBsum; 2N95; -.
DR   AlphaFoldDB; P46973; -.
DR   BMRB; P46973; -.
DR   SMR; P46973; -.
DR   BioGRID; 33810; 71.
DR   DIP; DIP-4241N; -.
DR   IntAct; P46973; 5.
DR   STRING; 4932.YJR055W; -.
DR   MaxQB; P46973; -.
DR   PaxDb; 4932-YJR055W; -.
DR   PeptideAtlas; P46973; -.
DR   EnsemblFungi; YJR055W_mRNA; YJR055W; YJR055W.
DR   GeneID; 853516; -.
DR   KEGG; sce:YJR055W; -.
DR   AGR; SGD:S000003816; -.
DR   SGD; S000003816; HIT1.
DR   VEuPathDB; FungiDB:YJR055W; -.
DR   eggNOG; ENOG502S27I; Eukaryota.
DR   HOGENOM; CLU_1619339_0_0_1; -.
DR   InParanoid; P46973; -.
DR   OMA; QYNTVKF; -.
DR   OrthoDB; 5487128at2759; -.
DR   BioCyc; YEAST:G3O-31689-MONOMER; -.
DR   BioGRID-ORCS; 853516; 7 hits in 10 CRISPR screens.
DR   PRO; PR:P46973; -.
DR   Proteomes; UP000002311; Chromosome X.
DR   RNAct; P46973; Protein.
DR   GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR   GO; GO:0005634; C:nucleus; HDA:SGD.
DR   GO; GO:0070761; C:pre-snoRNP complex; IBA:GO_Central.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0000492; P:box C/D snoRNP assembly; IMP:SGD.
DR   GO; GO:0000463; P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:SGD.
DR   GO; GO:0048254; P:snoRNA localization; IBA:GO_Central.
DR   Gene3D; 1.20.1440.260; -; 1.
DR   Gene3D; 3.30.60.190; -; 1.
DR   InterPro; IPR040722; Hit1_C.
DR   InterPro; IPR007529; Znf_HIT.
DR   Pfam; PF18268; Hit1_C; 1.
DR   Pfam; PF04438; zf-HIT; 1.
DR   SUPFAM; SSF144232; HIT/MYND zinc finger-like; 1.
DR   PROSITE; PS51083; ZF_HIT; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Metal-binding; Reference proteome; Zinc; Zinc-finger.
FT   CHAIN           1..164
FT                   /note="Protein HIT1"
FT                   /id="PRO_0000173556"
FT   ZN_FING         8..49
FT                   /note="HIT-type; degenerate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00453"
FT   BINDING         8
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00453"
FT   BINDING         11
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00453"
FT   BINDING         28
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00453"
FT   BINDING         32
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00453"
FT   CONFLICT        36..37
FT                   /note="AA -> RR (in Ref. 1; BAA01878)"
FT                   /evidence="ECO:0000305"
FT   TURN            9..11
FT                   /evidence="ECO:0007829|PDB:2N95"
FT   STRAND          13..15
FT                   /evidence="ECO:0007829|PDB:2N95"
FT   TURN            21..23
FT                   /evidence="ECO:0007829|PDB:2N95"
FT   STRAND          26..29
FT                   /evidence="ECO:0007829|PDB:2N95"
FT   HELIX           30..33
FT                   /evidence="ECO:0007829|PDB:2N95"
FT   STRAND          36..39
FT                   /evidence="ECO:0007829|PDB:2N95"
FT   HELIX           77..84
FT                   /evidence="ECO:0007829|PDB:2MJF"
FT   HELIX           87..92
FT                   /evidence="ECO:0007829|PDB:2MJF"
FT   HELIX           96..110
FT                   /evidence="ECO:0007829|PDB:2MJF"
FT   STRAND          119..122
FT                   /evidence="ECO:0007829|PDB:2MJF"
FT   HELIX           123..134
FT                   /evidence="ECO:0007829|PDB:2MJF"
FT   TURN            135..137
FT                   /evidence="ECO:0007829|PDB:2MJF"
FT   HELIX           146..160
FT                   /evidence="ECO:0007829|PDB:2MJF"
SQ   SEQUENCE   164 AA;  18376 MW;  EAC6ABB5457F87AA CRC64;
     MVSSAVKCGI CRGVDGKYKC PKCGVRYCSL KCYKDAAKHV HKESEQPRAG TEANVEVVNN
     DKIINSSLAM NKTLKTKAFD DIYQNSAELQ ELLKYNTVKF HLAKVYRILS STVNDGSSGK
     MNSDLQKELA VNYLNTLRYG GIHYNEAIEE FCQILLDKLN AVKK
//