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P46971

- PMT4_YEAST

UniProt

P46971 - PMT4_YEAST

Protein

Dolichyl-phosphate-mannose--protein mannosyltransferase 4

Gene

PMT4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 127 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Transfers mannose from Dol-P-mannose to Ser or Thr residues on proteins. Seems to be active on GAS1.

    Catalytic activityi

    Dolichyl phosphate D-mannose + protein = dolichyl phosphate + O-D-mannosylprotein.

    GO - Molecular functioni

    1. dolichyl-phosphate-mannose-protein mannosyltransferase activity Source: SGD
    2. identical protein binding Source: IntAct

    GO - Biological processi

    1. protein O-linked glycosylation Source: SGD
    2. protein O-linked mannosylation Source: SGD
    3. regulation of endoplasmic reticulum unfolded protein response Source: SGD

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciYEAST:YJR143C-MONOMER.
    BRENDAi2.4.1.109. 984.

    Protein family/group databases

    CAZyiGT39. Glycosyltransferase Family 39.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dolichyl-phosphate-mannose--protein mannosyltransferase 4 (EC:2.4.1.109)
    Gene namesi
    Name:PMT4
    Ordered Locus Names:YJR143C
    ORF Names:J2176
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome X

    Organism-specific databases

    CYGDiYJR143c.
    SGDiS000003904. PMT4.

    Subcellular locationi

    GO - Cellular componenti

    1. dolichyl-phosphate-mannose-protein mannosyltransferase Pmt4p homodimer complex Source: SGD
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 762762Dolichyl-phosphate-mannose--protein mannosyltransferase 4PRO_0000121494Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi759 – 7591N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiP46971.
    PaxDbiP46971.
    PeptideAtlasiP46971.

    Expressioni

    Gene expression databases

    GenevestigatoriP46971.

    Interactioni

    Subunit structurei

    Interacts with RCR1.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself2EBI-13585,EBI-13585

    Protein-protein interaction databases

    BioGridi33899. 70 interactions.
    DIPiDIP-2549N.
    IntActiP46971. 5 interactions.
    MINTiMINT-425890.
    STRINGi4932.YJR143C.

    Structurei

    3D structure databases

    ProteinModelPortaliP46971.
    SMRiP46971. Positions 330-436.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 5353LumenalSequence AnalysisAdd
    BLAST
    Topological domaini75 – 13662CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini158 – 1669LumenalSequence Analysis
    Topological domaini188 – 1892CytoplasmicSequence Analysis
    Topological domaini211 – 2177LumenalSequence Analysis
    Topological domaini239 – 2424CytoplasmicSequence Analysis
    Topological domaini264 – 28320LumenalSequence AnalysisAdd
    BLAST
    Topological domaini305 – 593289CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini615 – 63521LumenalSequence AnalysisAdd
    BLAST
    Topological domaini657 – 71660CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini738 – 76225LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei54 – 7421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei137 – 15721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei167 – 18721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei190 – 21021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei218 – 23821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei243 – 26321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei284 – 30421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei594 – 61421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei636 – 65621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei717 – 73721HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini331 – 39161MIR 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini399 – 45860MIR 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini464 – 52158MIR 3PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the glycosyltransferase 39 family.Curated
    Contains 3 MIR domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1928.
    GeneTreeiENSGT00740000116239.
    HOGENOMiHOG000157526.
    KOiK00728.
    OMAiYLERSYF.
    OrthoDBiEOG7BP89X.

    Family and domain databases

    InterProiIPR027005. GlyclTrfase_39_like.
    IPR003342. Glyco_trans_39.
    IPR016093. MIR_motif.
    [Graphical view]
    PANTHERiPTHR10050. PTHR10050. 1 hit.
    PfamiPF02815. MIR. 1 hit.
    PF02366. PMT. 1 hit.
    [Graphical view]
    SMARTiSM00472. MIR. 3 hits.
    [Graphical view]
    SUPFAMiSSF82109. SSF82109. 1 hit.
    PROSITEiPS50919. MIR. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P46971-1 [UniParc]FASTAAdd to Basket

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    MSVPKKRNHG KLPPSTKDVD DPSLKYTKAA PKCEQVAEHW LLQPLPEPES    50
    RYSFWVTIVT LLAFAARFYK IWYPKEVVFD EVHFGKFASY YLERSYFFDV 100
    HPPFAKMMIA FIGWLCGYDG SFKFDEIGYS YETHPAPYIA YRSFNAILGT 150
    LTVPIMFNTL KELNFRAITC AFASLLVAID TAHVTETRLI LLDAILIISI 200
    AATMYCYVRF YKCQLRQPFT WSWYIWLHAT GLSLSFVIST KYVGVMTYSA 250
    IGFAAVVNLW QLLDIKAGLS LRQFMRHFSK RLNGLVLIPF VIYLFWFWVH 300
    FTVLNTSGPG DAFMSAEFQE TLKDSPLSVD SKTVNYFDII TIKHQDTDAF 350
    LHSHLARYPQ RYEDGRISSA GQQVTGYTHP DFNNQWEVLP PHGSDVGKGQ 400
    AVLLNQHIRL RHVATDTYLL AHDVASPFYP TNEEITTVTL EEGDGELYPE 450
    TLFAFQPLKK SDEGHVLKSK TVSFRLFHVD TSVALWTHND ELLPDWGFQQ 500
    QEINGNKKVI DPSNNWVVDE IVNLDEVRKV YIPKVVKPLP FLKKWIETQK 550
    SMFEHNNKLS SEHPFASEPY SWPGSLSGVS FWTNGDEKKQ IYFIGNIIGW 600
    WFQVISLAVF VGIIVADLIT RHRGYYALNK MTREKLYGPL MFFFVSWCCH 650
    YFPFFLMARQ KFLHHYLPAH LIACLFSGAL WEVIFSDCKS LDLEKDEDIS 700
    GASYERNPKV YVKPYTVFLV CVSCAVAWFF VYFSPLVYGD VSLSPSEVVS 750
    REWFDIELNF SK 762
    Length:762
    Mass (Da):87,966
    Last modified:November 1, 1995 - v1
    Checksum:i0FBBEB283BBC0CBA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X83798 Genomic DNA. Translation: CAA58729.1.
    Z49643 Genomic DNA. Translation: CAA89676.1.
    BK006943 Genomic DNA. Translation: DAA08928.1.
    PIRiS60415.
    RefSeqiNP_012677.1. NM_001181801.1.

    Genome annotation databases

    EnsemblFungiiYJR143C; YJR143C; YJR143C.
    GeneIDi853608.
    KEGGisce:YJR143C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X83798 Genomic DNA. Translation: CAA58729.1 .
    Z49643 Genomic DNA. Translation: CAA89676.1 .
    BK006943 Genomic DNA. Translation: DAA08928.1 .
    PIRi S60415.
    RefSeqi NP_012677.1. NM_001181801.1.

    3D structure databases

    ProteinModelPortali P46971.
    SMRi P46971. Positions 330-436.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33899. 70 interactions.
    DIPi DIP-2549N.
    IntActi P46971. 5 interactions.
    MINTi MINT-425890.
    STRINGi 4932.YJR143C.

    Protein family/group databases

    CAZyi GT39. Glycosyltransferase Family 39.

    Proteomic databases

    MaxQBi P46971.
    PaxDbi P46971.
    PeptideAtlasi P46971.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YJR143C ; YJR143C ; YJR143C .
    GeneIDi 853608.
    KEGGi sce:YJR143C.

    Organism-specific databases

    CYGDi YJR143c.
    SGDi S000003904. PMT4.

    Phylogenomic databases

    eggNOGi COG1928.
    GeneTreei ENSGT00740000116239.
    HOGENOMi HOG000157526.
    KOi K00728.
    OMAi YLERSYF.
    OrthoDBi EOG7BP89X.

    Enzyme and pathway databases

    BioCyci YEAST:YJR143C-MONOMER.
    BRENDAi 2.4.1.109. 984.

    Miscellaneous databases

    NextBioi 974448.

    Gene expression databases

    Genevestigatori P46971.

    Family and domain databases

    InterProi IPR027005. GlyclTrfase_39_like.
    IPR003342. Glyco_trans_39.
    IPR016093. MIR_motif.
    [Graphical view ]
    PANTHERi PTHR10050. PTHR10050. 1 hit.
    Pfami PF02815. MIR. 1 hit.
    PF02366. PMT. 1 hit.
    [Graphical view ]
    SMARTi SM00472. MIR. 3 hits.
    [Graphical view ]
    SUPFAMi SSF82109. SSF82109. 1 hit.
    PROSITEi PS50919. MIR. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "PMT3 and PMT4, two new members of the protein-O-mannosyltransferase gene family of Saccharomyces cerevisiae."
      Immervoll T., Gentzsch M., Tanner W.
      Yeast 11:1345-1351(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "The PMT gene family: protein O-glycosylation in Saccharomyces cerevisiae is vital."
      Gaentzsch M., Tanner W.
      EMBO J. 15:5752-5759(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
      Kim H., Melen K., Oesterberg M., von Heijne G.
      Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: ATCC 208353 / W303-1A.
    7. "Peculiar protein-protein interactions of the novel endoplasmic reticulum membrane protein Rcr1 and ubiquitin ligase Rsp5."
      Imai K., Noda Y., Adachi H., Yoda K.
      Biosci. Biotechnol. Biochem. 71:249-252(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RCR1.

    Entry informationi

    Entry nameiPMT4_YEAST
    AccessioniPrimary (citable) accession number: P46971
    Secondary accession number(s): D6VWW2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 127 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1270 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3