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P46971 (PMT4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 126. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dolichyl-phosphate-mannose--protein mannosyltransferase 4

EC=2.4.1.109
Gene names
Name:PMT4
Ordered Locus Names:YJR143C
ORF Names:J2176
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length762 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transfers mannose from Dol-P-mannose to Ser or Thr residues on proteins. Seems to be active on GAS1.

Catalytic activity

Dolichyl phosphate D-mannose + protein = dolichyl phosphate + O-D-mannosylprotein.

Subunit structure

Interacts with RCR1. Ref.7

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.

Miscellaneous

Present with 1270 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the glycosyltransferase 39 family.

Contains 3 MIR domains.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself2EBI-13585,EBI-13585

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 762762Dolichyl-phosphate-mannose--protein mannosyltransferase 4
PRO_0000121494

Regions

Topological domain1 – 5353Lumenal Potential
Transmembrane54 – 7421Helical; Potential
Topological domain75 – 13662Cytoplasmic Potential
Transmembrane137 – 15721Helical; Potential
Topological domain158 – 1669Lumenal Potential
Transmembrane167 – 18721Helical; Potential
Topological domain188 – 1892Cytoplasmic Potential
Transmembrane190 – 21021Helical; Potential
Topological domain211 – 2177Lumenal Potential
Transmembrane218 – 23821Helical; Potential
Topological domain239 – 2424Cytoplasmic Potential
Transmembrane243 – 26321Helical; Potential
Topological domain264 – 28320Lumenal Potential
Transmembrane284 – 30421Helical; Potential
Topological domain305 – 593289Cytoplasmic Potential
Transmembrane594 – 61421Helical; Potential
Topological domain615 – 63521Lumenal Potential
Transmembrane636 – 65621Helical; Potential
Topological domain657 – 71660Cytoplasmic Potential
Transmembrane717 – 73721Helical; Potential
Topological domain738 – 76225Lumenal Potential
Domain331 – 39161MIR 1
Domain399 – 45860MIR 2
Domain464 – 52158MIR 3

Amino acid modifications

Glycosylation7591N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P46971 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 0FBBEB283BBC0CBA

FASTA76287,966
        10         20         30         40         50         60 
MSVPKKRNHG KLPPSTKDVD DPSLKYTKAA PKCEQVAEHW LLQPLPEPES RYSFWVTIVT 

        70         80         90        100        110        120 
LLAFAARFYK IWYPKEVVFD EVHFGKFASY YLERSYFFDV HPPFAKMMIA FIGWLCGYDG 

       130        140        150        160        170        180 
SFKFDEIGYS YETHPAPYIA YRSFNAILGT LTVPIMFNTL KELNFRAITC AFASLLVAID 

       190        200        210        220        230        240 
TAHVTETRLI LLDAILIISI AATMYCYVRF YKCQLRQPFT WSWYIWLHAT GLSLSFVIST 

       250        260        270        280        290        300 
KYVGVMTYSA IGFAAVVNLW QLLDIKAGLS LRQFMRHFSK RLNGLVLIPF VIYLFWFWVH 

       310        320        330        340        350        360 
FTVLNTSGPG DAFMSAEFQE TLKDSPLSVD SKTVNYFDII TIKHQDTDAF LHSHLARYPQ 

       370        380        390        400        410        420 
RYEDGRISSA GQQVTGYTHP DFNNQWEVLP PHGSDVGKGQ AVLLNQHIRL RHVATDTYLL 

       430        440        450        460        470        480 
AHDVASPFYP TNEEITTVTL EEGDGELYPE TLFAFQPLKK SDEGHVLKSK TVSFRLFHVD 

       490        500        510        520        530        540 
TSVALWTHND ELLPDWGFQQ QEINGNKKVI DPSNNWVVDE IVNLDEVRKV YIPKVVKPLP 

       550        560        570        580        590        600 
FLKKWIETQK SMFEHNNKLS SEHPFASEPY SWPGSLSGVS FWTNGDEKKQ IYFIGNIIGW 

       610        620        630        640        650        660 
WFQVISLAVF VGIIVADLIT RHRGYYALNK MTREKLYGPL MFFFVSWCCH YFPFFLMARQ 

       670        680        690        700        710        720 
KFLHHYLPAH LIACLFSGAL WEVIFSDCKS LDLEKDEDIS GASYERNPKV YVKPYTVFLV 

       730        740        750        760 
CVSCAVAWFF VYFSPLVYGD VSLSPSEVVS REWFDIELNF SK 

« Hide

References

« Hide 'large scale' references
[1]"PMT3 and PMT4, two new members of the protein-O-mannosyltransferase gene family of Saccharomyces cerevisiae."
Immervoll T., Gentzsch M., Tanner W.
Yeast 11:1345-1351(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"The PMT gene family: protein O-glycosylation in Saccharomyces cerevisiae is vital."
Gaentzsch M., Tanner W.
EMBO J. 15:5752-5759(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
[7]"Peculiar protein-protein interactions of the novel endoplasmic reticulum membrane protein Rcr1 and ubiquitin ligase Rsp5."
Imai K., Noda Y., Adachi H., Yoda K.
Biosci. Biotechnol. Biochem. 71:249-252(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RCR1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X83798 Genomic DNA. Translation: CAA58729.1.
Z49643 Genomic DNA. Translation: CAA89676.1.
BK006943 Genomic DNA. Translation: DAA08928.1.
PIRS60415.
RefSeqNP_012677.1. NM_001181801.1.

3D structure databases

ProteinModelPortalP46971.
SMRP46971. Positions 330-436.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33899. 70 interactions.
DIPDIP-2549N.
IntActP46971. 5 interactions.
MINTMINT-425890.
STRING4932.YJR143C.

Protein family/group databases

CAZyGT39. Glycosyltransferase Family 39.

Proteomic databases

MaxQBP46971.
PaxDbP46971.
PeptideAtlasP46971.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJR143C; YJR143C; YJR143C.
GeneID853608.
KEGGsce:YJR143C.

Organism-specific databases

CYGDYJR143c.
SGDS000003904. PMT4.

Phylogenomic databases

eggNOGCOG1928.
GeneTreeENSGT00740000116239.
HOGENOMHOG000157526.
KOK00728.
OMAYLERSYF.
OrthoDBEOG7BP89X.

Enzyme and pathway databases

BioCycYEAST:YJR143C-MONOMER.
BRENDA2.4.1.109. 984.

Gene expression databases

GenevestigatorP46971.

Family and domain databases

InterProIPR027005. GlyclTrfase_39_like.
IPR003342. Glyco_trans_39.
IPR016093. MIR_motif.
[Graphical view]
PANTHERPTHR10050. PTHR10050. 1 hit.
PfamPF02815. MIR. 1 hit.
PF02366. PMT. 1 hit.
[Graphical view]
SMARTSM00472. MIR. 3 hits.
[Graphical view]
SUPFAMSSF82109. SSF82109. 1 hit.
PROSITEPS50919. MIR. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio974448.

Entry information

Entry namePMT4_YEAST
AccessionPrimary (citable) accession number: P46971
Secondary accession number(s): D6VWW2
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 11, 2014
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families