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P46971

- PMT4_YEAST

UniProt

P46971 - PMT4_YEAST

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Protein
Dolichyl-phosphate-mannose--protein mannosyltransferase 4
Gene
PMT4, YJR143C, J2176
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Transfers mannose from Dol-P-mannose to Ser or Thr residues on proteins. Seems to be active on GAS1.

Catalytic activityi

Dolichyl phosphate D-mannose + protein = dolichyl phosphate + O-D-mannosylprotein.

GO - Molecular functioni

  1. dolichyl-phosphate-mannose-protein mannosyltransferase activity Source: SGD
  2. identical protein binding Source: IntAct
Complete GO annotation...

GO - Biological processi

  1. protein O-linked glycosylation Source: SGD
  2. protein O-linked mannosylation Source: SGD
  3. regulation of endoplasmic reticulum unfolded protein response Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciYEAST:YJR143C-MONOMER.
BRENDAi2.4.1.109. 984.

Protein family/group databases

CAZyiGT39. Glycosyltransferase Family 39.

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl-phosphate-mannose--protein mannosyltransferase 4 (EC:2.4.1.109)
Gene namesi
Name:PMT4
Ordered Locus Names:YJR143C
ORF Names:J2176
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

CYGDiYJR143c.
SGDiS000003904. PMT4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5353Lumenal Reviewed prediction
Add
BLAST
Transmembranei54 – 7421Helical; Reviewed prediction
Add
BLAST
Topological domaini75 – 13662Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei137 – 15721Helical; Reviewed prediction
Add
BLAST
Topological domaini158 – 1669Lumenal Reviewed prediction
Transmembranei167 – 18721Helical; Reviewed prediction
Add
BLAST
Topological domaini188 – 1892Cytoplasmic Reviewed prediction
Transmembranei190 – 21021Helical; Reviewed prediction
Add
BLAST
Topological domaini211 – 2177Lumenal Reviewed prediction
Transmembranei218 – 23821Helical; Reviewed prediction
Add
BLAST
Topological domaini239 – 2424Cytoplasmic Reviewed prediction
Transmembranei243 – 26321Helical; Reviewed prediction
Add
BLAST
Topological domaini264 – 28320Lumenal Reviewed prediction
Add
BLAST
Transmembranei284 – 30421Helical; Reviewed prediction
Add
BLAST
Topological domaini305 – 593289Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei594 – 61421Helical; Reviewed prediction
Add
BLAST
Topological domaini615 – 63521Lumenal Reviewed prediction
Add
BLAST
Transmembranei636 – 65621Helical; Reviewed prediction
Add
BLAST
Topological domaini657 – 71660Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei717 – 73721Helical; Reviewed prediction
Add
BLAST
Topological domaini738 – 76225Lumenal Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. dolichyl-phosphate-mannose-protein mannosyltransferase Pmt4p homodimer complex Source: SGD
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 762762Dolichyl-phosphate-mannose--protein mannosyltransferase 4
PRO_0000121494Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi759 – 7591N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP46971.
PaxDbiP46971.
PeptideAtlasiP46971.

Expressioni

Gene expression databases

GenevestigatoriP46971.

Interactioni

Subunit structurei

Interacts with RCR1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-13585,EBI-13585

Protein-protein interaction databases

BioGridi33899. 70 interactions.
DIPiDIP-2549N.
IntActiP46971. 5 interactions.
MINTiMINT-425890.
STRINGi4932.YJR143C.

Structurei

3D structure databases

ProteinModelPortaliP46971.
SMRiP46971. Positions 330-436.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini331 – 39161MIR 1
Add
BLAST
Domaini399 – 45860MIR 2
Add
BLAST
Domaini464 – 52158MIR 3
Add
BLAST

Sequence similaritiesi

Contains 3 MIR domains.

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1928.
GeneTreeiENSGT00740000116239.
HOGENOMiHOG000157526.
KOiK00728.
OMAiYLERSYF.
OrthoDBiEOG7BP89X.

Family and domain databases

InterProiIPR027005. GlyclTrfase_39_like.
IPR003342. Glyco_trans_39.
IPR016093. MIR_motif.
[Graphical view]
PANTHERiPTHR10050. PTHR10050. 1 hit.
PfamiPF02815. MIR. 1 hit.
PF02366. PMT. 1 hit.
[Graphical view]
SMARTiSM00472. MIR. 3 hits.
[Graphical view]
SUPFAMiSSF82109. SSF82109. 1 hit.
PROSITEiPS50919. MIR. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P46971-1 [UniParc]FASTAAdd to Basket

« Hide

MSVPKKRNHG KLPPSTKDVD DPSLKYTKAA PKCEQVAEHW LLQPLPEPES    50
RYSFWVTIVT LLAFAARFYK IWYPKEVVFD EVHFGKFASY YLERSYFFDV 100
HPPFAKMMIA FIGWLCGYDG SFKFDEIGYS YETHPAPYIA YRSFNAILGT 150
LTVPIMFNTL KELNFRAITC AFASLLVAID TAHVTETRLI LLDAILIISI 200
AATMYCYVRF YKCQLRQPFT WSWYIWLHAT GLSLSFVIST KYVGVMTYSA 250
IGFAAVVNLW QLLDIKAGLS LRQFMRHFSK RLNGLVLIPF VIYLFWFWVH 300
FTVLNTSGPG DAFMSAEFQE TLKDSPLSVD SKTVNYFDII TIKHQDTDAF 350
LHSHLARYPQ RYEDGRISSA GQQVTGYTHP DFNNQWEVLP PHGSDVGKGQ 400
AVLLNQHIRL RHVATDTYLL AHDVASPFYP TNEEITTVTL EEGDGELYPE 450
TLFAFQPLKK SDEGHVLKSK TVSFRLFHVD TSVALWTHND ELLPDWGFQQ 500
QEINGNKKVI DPSNNWVVDE IVNLDEVRKV YIPKVVKPLP FLKKWIETQK 550
SMFEHNNKLS SEHPFASEPY SWPGSLSGVS FWTNGDEKKQ IYFIGNIIGW 600
WFQVISLAVF VGIIVADLIT RHRGYYALNK MTREKLYGPL MFFFVSWCCH 650
YFPFFLMARQ KFLHHYLPAH LIACLFSGAL WEVIFSDCKS LDLEKDEDIS 700
GASYERNPKV YVKPYTVFLV CVSCAVAWFF VYFSPLVYGD VSLSPSEVVS 750
REWFDIELNF SK 762
Length:762
Mass (Da):87,966
Last modified:November 1, 1995 - v1
Checksum:i0FBBEB283BBC0CBA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X83798 Genomic DNA. Translation: CAA58729.1.
Z49643 Genomic DNA. Translation: CAA89676.1.
BK006943 Genomic DNA. Translation: DAA08928.1.
PIRiS60415.
RefSeqiNP_012677.1. NM_001181801.1.

Genome annotation databases

EnsemblFungiiYJR143C; YJR143C; YJR143C.
GeneIDi853608.
KEGGisce:YJR143C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X83798 Genomic DNA. Translation: CAA58729.1 .
Z49643 Genomic DNA. Translation: CAA89676.1 .
BK006943 Genomic DNA. Translation: DAA08928.1 .
PIRi S60415.
RefSeqi NP_012677.1. NM_001181801.1.

3D structure databases

ProteinModelPortali P46971.
SMRi P46971. Positions 330-436.
ModBasei Search...

Protein-protein interaction databases

BioGridi 33899. 70 interactions.
DIPi DIP-2549N.
IntActi P46971. 5 interactions.
MINTi MINT-425890.
STRINGi 4932.YJR143C.

Protein family/group databases

CAZyi GT39. Glycosyltransferase Family 39.

Proteomic databases

MaxQBi P46971.
PaxDbi P46971.
PeptideAtlasi P46971.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YJR143C ; YJR143C ; YJR143C .
GeneIDi 853608.
KEGGi sce:YJR143C.

Organism-specific databases

CYGDi YJR143c.
SGDi S000003904. PMT4.

Phylogenomic databases

eggNOGi COG1928.
GeneTreei ENSGT00740000116239.
HOGENOMi HOG000157526.
KOi K00728.
OMAi YLERSYF.
OrthoDBi EOG7BP89X.

Enzyme and pathway databases

BioCyci YEAST:YJR143C-MONOMER.
BRENDAi 2.4.1.109. 984.

Miscellaneous databases

NextBioi 974448.

Gene expression databases

Genevestigatori P46971.

Family and domain databases

InterProi IPR027005. GlyclTrfase_39_like.
IPR003342. Glyco_trans_39.
IPR016093. MIR_motif.
[Graphical view ]
PANTHERi PTHR10050. PTHR10050. 1 hit.
Pfami PF02815. MIR. 1 hit.
PF02366. PMT. 1 hit.
[Graphical view ]
SMARTi SM00472. MIR. 3 hits.
[Graphical view ]
SUPFAMi SSF82109. SSF82109. 1 hit.
PROSITEi PS50919. MIR. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "PMT3 and PMT4, two new members of the protein-O-mannosyltransferase gene family of Saccharomyces cerevisiae."
    Immervoll T., Gentzsch M., Tanner W.
    Yeast 11:1345-1351(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The PMT gene family: protein O-glycosylation in Saccharomyces cerevisiae is vital."
    Gaentzsch M., Tanner W.
    EMBO J. 15:5752-5759(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  7. "Peculiar protein-protein interactions of the novel endoplasmic reticulum membrane protein Rcr1 and ubiquitin ligase Rsp5."
    Imai K., Noda Y., Adachi H., Yoda K.
    Biosci. Biotechnol. Biochem. 71:249-252(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RCR1.

Entry informationi

Entry nameiPMT4_YEAST
AccessioniPrimary (citable) accession number: P46971
Secondary accession number(s): D6VWW2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 11, 2014
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1270 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3

Similar proteinsi