Dolichyl-phosphate-mannose--protein mannosyltransferase 4 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P46971 (PMT4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 116. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Dolichyl-phosphate-mannose--protein mannosyltransferase 4
EC=2.4.1.109

Gene names

Name:	PMT4
Ordered Locus Names:	YJR143C
ORF Names:	J2176

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	762 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transfers mannose from Dol-P-mannose to Ser or Thr residues on proteins. Seems to be active on GAS1.
Catalytic activity	Dolichyl phosphate D-mannose + protein = dolichyl phosphate + O-D-mannosylprotein.
Subunit structure	Interacts with RCR1. Ref.7
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Miscellaneous	Present with 1270 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the glycosyltransferase 39 family. Contains 3 MIR domains.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Repeat Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein O-linked mannosylation Inferred from mutant phenotype PubMed 18182384 PubMed 21231968. Source: SGD regulation of endoplasmic reticulum unfolded protein response Inferred from genetic interaction PubMed 21231968. Source: SGD
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD
Molecular_function	dolichyl-phosphate-mannose-protein mannosyltransferase activity Inferred from mutant phenotype PubMed 21956107 Ref.1. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 762

762

Dolichyl-phosphate-mannose--protein mannosyltransferase 4

PRO_0000121494

Regions

Topological domain

1 – 53

Lumenal Potential

Transmembrane

54 – 74

Helical; Potential

Topological domain

75 – 136

Cytoplasmic Potential

Transmembrane

137 – 157

Helical; Potential

Topological domain

158 – 166

Lumenal Potential

Transmembrane

167 – 187

Helical; Potential

Topological domain

188 – 189

Cytoplasmic Potential

Transmembrane

190 – 210

Helical; Potential

Topological domain

211 – 217

Lumenal Potential

Transmembrane

218 – 238

Helical; Potential

Topological domain

239 – 242

Cytoplasmic Potential

Transmembrane

243 – 263

Helical; Potential

Topological domain

264 – 283

Lumenal Potential

Transmembrane

284 – 304

Helical; Potential

Topological domain

305 – 593

289

Cytoplasmic Potential

Transmembrane

594 – 614

Helical; Potential

Topological domain

615 – 635

Lumenal Potential

Transmembrane

636 – 656

Helical; Potential

Topological domain

657 – 716

Cytoplasmic Potential

Transmembrane

717 – 737

Helical; Potential

Topological domain

738 – 762

Lumenal Potential

Domain

331 – 391

MIR 1

Domain

399 – 458

MIR 2

Domain

464 – 521

MIR 3

Amino acid modifications

Glycosylation

759

N-linked (GlcNAc...) Potential

Sequences

Sequence

Length

Mass (Da)

Tools

P46971 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 0FBBEB283BBC0CBA
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FASTA

762

87,966

        10         20         30         40         50         60 
MSVPKKRNHG KLPPSTKDVD DPSLKYTKAA PKCEQVAEHW LLQPLPEPES RYSFWVTIVT 

        70         80         90        100        110        120 
LLAFAARFYK IWYPKEVVFD EVHFGKFASY YLERSYFFDV HPPFAKMMIA FIGWLCGYDG 

       130        140        150        160        170        180 
SFKFDEIGYS YETHPAPYIA YRSFNAILGT LTVPIMFNTL KELNFRAITC AFASLLVAID 

       190        200        210        220        230        240 
TAHVTETRLI LLDAILIISI AATMYCYVRF YKCQLRQPFT WSWYIWLHAT GLSLSFVIST 

       250        260        270        280        290        300 
KYVGVMTYSA IGFAAVVNLW QLLDIKAGLS LRQFMRHFSK RLNGLVLIPF VIYLFWFWVH 

       310        320        330        340        350        360 
FTVLNTSGPG DAFMSAEFQE TLKDSPLSVD SKTVNYFDII TIKHQDTDAF LHSHLARYPQ 

       370        380        390        400        410        420 
RYEDGRISSA GQQVTGYTHP DFNNQWEVLP PHGSDVGKGQ AVLLNQHIRL RHVATDTYLL 

       430        440        450        460        470        480 
AHDVASPFYP TNEEITTVTL EEGDGELYPE TLFAFQPLKK SDEGHVLKSK TVSFRLFHVD 

       490        500        510        520        530        540 
TSVALWTHND ELLPDWGFQQ QEINGNKKVI DPSNNWVVDE IVNLDEVRKV YIPKVVKPLP 

       550        560        570        580        590        600 
FLKKWIETQK SMFEHNNKLS SEHPFASEPY SWPGSLSGVS FWTNGDEKKQ IYFIGNIIGW 

       610        620        630        640        650        660 
WFQVISLAVF VGIIVADLIT RHRGYYALNK MTREKLYGPL MFFFVSWCCH YFPFFLMARQ 

       670        680        690        700        710        720 
KFLHHYLPAH LIACLFSGAL WEVIFSDCKS LDLEKDEDIS GASYERNPKV YVKPYTVFLV 

       730        740        750        760 
CVSCAVAWFF VYFSPLVYGD VSLSPSEVVS REWFDIELNF SK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"PMT3 and PMT4, two new members of the protein-O-mannosyltransferase gene family of Saccharomyces cerevisiae." Immervoll T., Gentzsch M., Tanner W. Yeast 11:1345-1351(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"The PMT gene family: protein O-glycosylation in Saccharomyces cerevisiae is vital." Gaentzsch M., Tanner W. EMBO J. 15:5752-5759(1996) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
[7]	"Peculiar protein-protein interactions of the novel endoplasmic reticulum membrane protein Rcr1 and ubiquitin ligase Rsp5." Imai K., Noda Y., Adachi H., Yoda K. Biosci. Biotechnol. Biochem. 71:249-252(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH RCR1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X83798 Genomic DNA. Translation: CAA58729.1. Z49643 Genomic DNA. Translation: CAA89676.1. BK006943 Genomic DNA. Translation: DAA08928.1.
PIR	S60415.
RefSeq	NP_012677.1. NM_001181801.1.
3D structure databases
ProteinModelPortal	P46971.
SMR	P46971. Positions 330-436.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2549N.
IntAct	P46971. 4 interactions.
MINT	MINT-425890.
STRING	4932.YJR143C.
Protein family/group databases
CAZy	GT39. Glycosyltransferase Family 39.
Proteomic databases
PaxDb	P46971.
PeptideAtlas	P46971.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJR143C; YJR143C; YJR143C.
GeneID	853608.
KEGG	sce:YJR143C.
Organism-specific databases
CYGD	YJR143c.
SGD	S000003904. PMT4.
Phylogenomic databases
eggNOG	COG1928.
GeneTree	ENSGT00700000104567.
HOGENOM	HOG000157526.
KO	K00728.
OMA	GAYVEDI.
OrthoDB	EOG4ZCXCQ.
Enzyme and pathway databases
BioCyc	YEAST:YJR143C-MONOMER.
BRENDA	2.4.1.109. 984.
Gene expression databases
Genevestigator	P46971.
GermOnline	YJR143C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR027005. GlyclTrfase_39_like. IPR003342. Glyco_trans_39. IPR016093. MIR_motif. [Graphical view]
PANTHER	PTHR10050. PTHR10050. 1 hit.
Pfam	PF02815. MIR. 1 hit. PF02366. PMT. 1 hit. [Graphical view]
SMART	SM00472. MIR. 3 hits. [Graphical view]
SUPFAM	SSF82109. MIR. 1 hit.
PROSITE	PS50919. MIR. 3 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	974448.

Entry information

Entry name

PMT4_YEAST

Accession

Primary (citable) accession number: P46971
Secondary accession number(s): D6VWW2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 29, 2013
This is version 116 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents