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Protein

Dolichyl-phosphate-mannose--protein mannosyltransferase 4

Gene

PMT4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein O-mannosyltransferase involved in O-glycosylation which is essential for cell wall rigidity. Forms a homodimeric complex to transfer mannose from Dol-P-mannose to Ser or Thr residues on proteins. Specifically acts on secretory proteins with an ER-luminally oriented Ser/Thr-rich region flanked by a membrane anchor such as FUS1, AXL2, GAS1, KEX2, MID2, WSC1, WSC2, OPY2, PRM5, RAX2, or YNL176.4 Publications

Catalytic activityi

Dolichyl D-mannosyl phosphate + protein = dolichyl phosphate + O-D-mannosylprotein.1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.Curated
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

  • dolichyl-phosphate-mannose-protein mannosyltransferase activity Source: SGD

GO - Biological processi

  • ER-associated misfolded protein catabolic process Source: GO_Central
  • fungal-type cell wall organization Source: GO_Central
  • protein O-linked glycosylation Source: SGD
  • protein O-linked mannosylation Source: SGD
  • regulation of endoplasmic reticulum unfolded protein response Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciYEAST:YJR143C-MONOMER.
BRENDAi2.4.1.109. 984.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT39. Glycosyltransferase Family 39.

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl-phosphate-mannose--protein mannosyltransferase 4 (EC:2.4.1.1091 Publication)
Gene namesi
Name:PMT41 Publication
Ordered Locus Names:YJR143CImported
ORF Names:J2176
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJR143C.
SGDiS000003904. PMT4.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 53LumenalSequence analysisAdd BLAST53
Transmembranei54 – 74HelicalSequence analysisAdd BLAST21
Topological domaini75 – 136CytoplasmicSequence analysisAdd BLAST62
Transmembranei137 – 157HelicalSequence analysisAdd BLAST21
Topological domaini158 – 166LumenalSequence analysis9
Transmembranei167 – 187HelicalSequence analysisAdd BLAST21
Topological domaini188 – 189CytoplasmicSequence analysis2
Transmembranei190 – 210HelicalSequence analysisAdd BLAST21
Topological domaini211 – 217LumenalSequence analysis7
Transmembranei218 – 238HelicalSequence analysisAdd BLAST21
Topological domaini239 – 242CytoplasmicSequence analysis4
Transmembranei243 – 263HelicalSequence analysisAdd BLAST21
Topological domaini264 – 283LumenalSequence analysisAdd BLAST20
Transmembranei284 – 304HelicalSequence analysisAdd BLAST21
Topological domaini305 – 593CytoplasmicSequence analysisAdd BLAST289
Transmembranei594 – 614HelicalSequence analysisAdd BLAST21
Topological domaini615 – 635LumenalSequence analysisAdd BLAST21
Transmembranei636 – 656HelicalSequence analysisAdd BLAST21
Topological domaini657 – 716CytoplasmicSequence analysisAdd BLAST60
Transmembranei717 – 737HelicalSequence analysisAdd BLAST21
Topological domaini738 – 762LumenalSequence analysisAdd BLAST25

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Leads to diminished MID2 O-mannosylation.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001214941 – 762Dolichyl-phosphate-mannose--protein mannosyltransferase 4Add BLAST762

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi759N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP46971.
PRIDEiP46971.

Interactioni

Subunit structurei

Forms a functional homodimer and may form a heterodimer with PMT6. Interacts with RCR1.1 Publication2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-13585,EBI-13585

Protein-protein interaction databases

BioGridi33899. 69 interactors.
DIPiDIP-2549N.
IntActiP46971. 5 interactors.
MINTiMINT-425890.

Structurei

3D structure databases

ProteinModelPortaliP46971.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini331 – 391MIR 1PROSITE-ProRule annotationAdd BLAST61
Domaini399 – 458MIR 2PROSITE-ProRule annotationAdd BLAST60
Domaini464 – 521MIR 3PROSITE-ProRule annotationAdd BLAST58

Sequence similaritiesi

Belongs to the glycosyltransferase 39 family.Curated
Contains 3 MIR domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00860000133866.
HOGENOMiHOG000157526.
InParanoidiP46971.
KOiK00728.
OMAiYLERSYF.
OrthoDBiEOG092C0JF4.

Family and domain databases

InterProiIPR027005. GlyclTrfase_39-like.
IPR003342. Glyco_trans_39/83.
IPR016093. MIR_motif.
IPR032421. PMT_4TMC.
[Graphical view]
PANTHERiPTHR10050. PTHR10050. 1 hit.
PfamiPF02815. MIR. 1 hit.
PF02366. PMT. 1 hit.
PF16192. PMT_4TMC. 1 hit.
[Graphical view]
SMARTiSM00472. MIR. 3 hits.
[Graphical view]
SUPFAMiSSF82109. SSF82109. 1 hit.
PROSITEiPS50919. MIR. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P46971-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVPKKRNHG KLPPSTKDVD DPSLKYTKAA PKCEQVAEHW LLQPLPEPES
60 70 80 90 100
RYSFWVTIVT LLAFAARFYK IWYPKEVVFD EVHFGKFASY YLERSYFFDV
110 120 130 140 150
HPPFAKMMIA FIGWLCGYDG SFKFDEIGYS YETHPAPYIA YRSFNAILGT
160 170 180 190 200
LTVPIMFNTL KELNFRAITC AFASLLVAID TAHVTETRLI LLDAILIISI
210 220 230 240 250
AATMYCYVRF YKCQLRQPFT WSWYIWLHAT GLSLSFVIST KYVGVMTYSA
260 270 280 290 300
IGFAAVVNLW QLLDIKAGLS LRQFMRHFSK RLNGLVLIPF VIYLFWFWVH
310 320 330 340 350
FTVLNTSGPG DAFMSAEFQE TLKDSPLSVD SKTVNYFDII TIKHQDTDAF
360 370 380 390 400
LHSHLARYPQ RYEDGRISSA GQQVTGYTHP DFNNQWEVLP PHGSDVGKGQ
410 420 430 440 450
AVLLNQHIRL RHVATDTYLL AHDVASPFYP TNEEITTVTL EEGDGELYPE
460 470 480 490 500
TLFAFQPLKK SDEGHVLKSK TVSFRLFHVD TSVALWTHND ELLPDWGFQQ
510 520 530 540 550
QEINGNKKVI DPSNNWVVDE IVNLDEVRKV YIPKVVKPLP FLKKWIETQK
560 570 580 590 600
SMFEHNNKLS SEHPFASEPY SWPGSLSGVS FWTNGDEKKQ IYFIGNIIGW
610 620 630 640 650
WFQVISLAVF VGIIVADLIT RHRGYYALNK MTREKLYGPL MFFFVSWCCH
660 670 680 690 700
YFPFFLMARQ KFLHHYLPAH LIACLFSGAL WEVIFSDCKS LDLEKDEDIS
710 720 730 740 750
GASYERNPKV YVKPYTVFLV CVSCAVAWFF VYFSPLVYGD VSLSPSEVVS
760
REWFDIELNF SK
Length:762
Mass (Da):87,966
Last modified:November 1, 1995 - v1
Checksum:i0FBBEB283BBC0CBA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83798 Genomic DNA. Translation: CAA58729.1.
Z49643 Genomic DNA. Translation: CAA89676.1.
BK006943 Genomic DNA. Translation: DAA08928.1.
PIRiS60415.
RefSeqiNP_012677.1. NM_001181801.1.

Genome annotation databases

EnsemblFungiiYJR143C; YJR143C; YJR143C.
GeneIDi853608.
KEGGisce:YJR143C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83798 Genomic DNA. Translation: CAA58729.1.
Z49643 Genomic DNA. Translation: CAA89676.1.
BK006943 Genomic DNA. Translation: DAA08928.1.
PIRiS60415.
RefSeqiNP_012677.1. NM_001181801.1.

3D structure databases

ProteinModelPortaliP46971.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33899. 69 interactors.
DIPiDIP-2549N.
IntActiP46971. 5 interactors.
MINTiMINT-425890.

Protein family/group databases

CAZyiGT39. Glycosyltransferase Family 39.

Proteomic databases

MaxQBiP46971.
PRIDEiP46971.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJR143C; YJR143C; YJR143C.
GeneIDi853608.
KEGGisce:YJR143C.

Organism-specific databases

EuPathDBiFungiDB:YJR143C.
SGDiS000003904. PMT4.

Phylogenomic databases

GeneTreeiENSGT00860000133866.
HOGENOMiHOG000157526.
InParanoidiP46971.
KOiK00728.
OMAiYLERSYF.
OrthoDBiEOG092C0JF4.

Enzyme and pathway databases

UniPathwayiUPA00378.
BioCyciYEAST:YJR143C-MONOMER.
BRENDAi2.4.1.109. 984.

Miscellaneous databases

PROiP46971.

Family and domain databases

InterProiIPR027005. GlyclTrfase_39-like.
IPR003342. Glyco_trans_39/83.
IPR016093. MIR_motif.
IPR032421. PMT_4TMC.
[Graphical view]
PANTHERiPTHR10050. PTHR10050. 1 hit.
PfamiPF02815. MIR. 1 hit.
PF02366. PMT. 1 hit.
PF16192. PMT_4TMC. 1 hit.
[Graphical view]
SMARTiSM00472. MIR. 3 hits.
[Graphical view]
SUPFAMiSSF82109. SSF82109. 1 hit.
PROSITEiPS50919. MIR. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPMT4_YEAST
AccessioniPrimary (citable) accession number: P46971
Secondary accession number(s): D6VWW2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 30, 2016
This is version 146 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1270 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.