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P46970

- NMD5_YEAST

UniProt

P46970 - NMD5_YEAST

Protein

Nonsense-mediated mRNA decay protein 5

Gene

NMD5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 2 (21 Sep 2011)
      Previous versions | rss
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    Functioni

    Active in protein import into the nucleus. Its major import substrate is transcription elongation factor TFIIS.1 Publication

    GO - Molecular functioni

    1. protein transporter activity Source: SGD

    GO - Biological processi

    1. protein import into nucleus Source: SGD

    Keywords - Biological processi

    Protein transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31750-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nonsense-mediated mRNA decay protein 5
    Alternative name(s):
    Karyopherin-119
    Gene namesi
    Name:NMD5
    Synonyms:KAP119
    Ordered Locus Names:YJR132W
    ORF Names:J2112
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome X

    Organism-specific databases

    CYGDiYJR132w.
    SGDiS000003893. NMD5.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: SGD
    2. nucleus Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10481048Nonsense-mediated mRNA decay protein 5PRO_0000096880Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei977 – 9771Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP46970.
    PaxDbiP46970.
    PeptideAtlasiP46970.

    Expressioni

    Gene expression databases

    GenevestigatoriP46970.

    Interactioni

    Subunit structurei

    GTP-bound Ran dissociates the isolated NMD5/TFIIS complex.1 Publication

    Protein-protein interaction databases

    BioGridi33887. 73 interactions.
    DIPiDIP-2326N.
    IntActiP46970. 36 interactions.
    MINTiMINT-561722.
    STRINGi4932.YJR132W.

    Structurei

    3D structure databases

    ProteinModelPortaliP46970.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini24 – 10481Importin N-terminalPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 importin N-terminal domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5656.
    GeneTreeiENSGT00550000074736.
    HOGENOMiHOG000248078.
    OMAiYKLFKSD.
    OrthoDBiEOG7QK0MD.

    Family and domain databases

    Gene3Di1.25.10.10. 2 hits.
    InterProiIPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR001494. Importin-beta_N.
    [Graphical view]
    PfamiPF03810. IBN_N. 1 hit.
    [Graphical view]
    SMARTiSM00913. IBN_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF48371. SSF48371. 1 hit.
    PROSITEiPS50166. IMPORTIN_B_NT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P46970-1 [UniParc]FASTAAdd to Basket

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    MDITELLQCF ACTLDHNAAV RTNAETHLKN ASKVPGFLGA CLDIIAADEV     50
    PENIKLSASL YFKNKITYGW SAGARQGSNE LLDSHVDPDE KPVVKDMLIK 100
    TMVSVSKTSP RCIRVLKSAL TVIISEDYPS KKWGNLLPNS LELLANEDIT 150
    VTYVGLLCLA EIFRTYRWKN NDERQDLEEL ILNYFPALLN YGANVLFQDG 200
    KYMNNEQIGE LVKLIIKIYK FVSYHDLPFT LQRSESFTPW ACFFVSIIQQ 250
    PLPQEVLAIS DIEVRSKNPW VKCKKWALAN LYRLFQRYAS TSLTRKFQYD 300
    EFKQMYCEEF LTQFLQVVFD QIEKWGTGQL WLSDECLYYI LNFVEQCVVQ 350
    KTTWKLVGPH YNVILQHVIF PLLKPTAETL EAFDNDPQEY INRNMDFWDV 400
    GYSPDLAALA LLTTCVTKRG KTTLQPTLEF MVSTLQSAVG DYNNIMLDNA 450
    LQIESCLRIF SSIIDRLITK DSPFASEMEK FILTYVLPFF KSQYGFLQSR 500
    VCDICSKLGS MDFKDPVITS TIYEGVMNCL NNSSNSLPVE LTAALALQTF 550
    ISDDQFNMKL SEHVVPTMQK LLSLSNDFES DVISGVMQDF VEQFAEQLQP 600
    FGVELMNTLV QQFLKLAIDL HETSNLDPDS FTNVDSIPDE SDKQMAALGI 650
    LSTTISILLS FENSPEILKN LEQSFYPAAE FILKNDIEDF YRECCEFVEN 700
    STFLLRDITP ISWKILELIG ECNRKPDSMV SYYLSDFMLA LNNILIYGRN 750
    ELKKNEFYTK IIFEIYQKAV TAEDNSLDDL RVVFDLSQEL VLALDDSLPQ 800
    QYRERLLADV VGSILTQKNE LKTNVVFSVT AFNVVISNMI TEPLITLQYL 850
    KQQGCLEIFF QTWITDYIPN YKRCYDIKLS VLALLKIILK LESNDYSVLN 900
    LENLVPQLGS IVTQLASRLP TALRQLANQR KEFSSSGFEE DTKWDENFLD 950
    VGDDDENDDE GDLTEKYLEL IKNRADSLDF VDGYDAKETF DDLEEDPLTG 1000
    SILDTVDVYK VFKESIANLQ HVDSNRYQGI LRHLTPADQE LFMGIMNA 1048
    Length:1,048
    Mass (Da):119,967
    Last modified:September 21, 2011 - v2
    Checksum:i90312C1AAE515949
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti258 – 2581A → S in CAA89663. (PubMed:8641269)Curated
    Sequence conflicti517 – 5171V → I in AAA74490. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U31375 Genomic DNA. Translation: AAA74490.1.
    Z49632 Genomic DNA. Translation: CAA89663.1.
    BK006943 Genomic DNA. Translation: DAA08916.2.
    PIRiS57155.
    RefSeqiNP_012666.2. NM_001181790.2.

    Genome annotation databases

    EnsemblFungiiYJR132W; YJR132W; YJR132W.
    GeneIDi853596.
    KEGGisce:YJR132W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U31375 Genomic DNA. Translation: AAA74490.1 .
    Z49632 Genomic DNA. Translation: CAA89663.1 .
    BK006943 Genomic DNA. Translation: DAA08916.2 .
    PIRi S57155.
    RefSeqi NP_012666.2. NM_001181790.2.

    3D structure databases

    ProteinModelPortali P46970.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33887. 73 interactions.
    DIPi DIP-2326N.
    IntActi P46970. 36 interactions.
    MINTi MINT-561722.
    STRINGi 4932.YJR132W.

    Proteomic databases

    MaxQBi P46970.
    PaxDbi P46970.
    PeptideAtlasi P46970.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YJR132W ; YJR132W ; YJR132W .
    GeneIDi 853596.
    KEGGi sce:YJR132W.

    Organism-specific databases

    CYGDi YJR132w.
    SGDi S000003893. NMD5.

    Phylogenomic databases

    eggNOGi COG5656.
    GeneTreei ENSGT00550000074736.
    HOGENOMi HOG000248078.
    OMAi YKLFKSD.
    OrthoDBi EOG7QK0MD.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31750-MONOMER.

    Miscellaneous databases

    NextBioi 974412.

    Gene expression databases

    Genevestigatori P46970.

    Family and domain databases

    Gene3Di 1.25.10.10. 2 hits.
    InterProi IPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR001494. Importin-beta_N.
    [Graphical view ]
    Pfami PF03810. IBN_N. 1 hit.
    [Graphical view ]
    SMARTi SM00913. IBN_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48371. SSF48371. 1 hit.
    PROSITEi PS50166. IMPORTIN_B_NT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. He F., Jacobson A.
      Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 258.
      Strain: ATCC 204508 / S288c.
    4. "A novel nuclear import pathway for the transcription factor TFIIS."
      Albertini M., Pemberton L.F., Rosenblum J.S., Blobel G.
      J. Cell Biol. 143:1447-1455(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, SUBUNIT.
    5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-977, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-977, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiNMD5_YEAST
    AccessioniPrimary (citable) accession number: P46970
    Secondary accession number(s): D6VWV0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: September 21, 2011
    Last modified: October 1, 2014
    This is version 112 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 13500 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3