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P46970

- NMD5_YEAST

UniProt

P46970 - NMD5_YEAST

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Protein

Nonsense-mediated mRNA decay protein 5

Gene

NMD5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Active in protein import into the nucleus. Its major import substrate is transcription elongation factor TFIIS.1 Publication

GO - Molecular functioni

  1. protein transporter activity Source: SGD

GO - Biological processi

  1. protein import into nucleus Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31750-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nonsense-mediated mRNA decay protein 5
Alternative name(s):
Karyopherin-119
Gene namesi
Name:NMD5
Synonyms:KAP119
Ordered Locus Names:YJR132W
ORF Names:J2112
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

CYGDiYJR132w.
SGDiS000003893. NMD5.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: SGD
  2. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10481048Nonsense-mediated mRNA decay protein 5PRO_0000096880Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei977 – 9771Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP46970.
PaxDbiP46970.
PeptideAtlasiP46970.

Expressioni

Gene expression databases

GenevestigatoriP46970.

Interactioni

Subunit structurei

GTP-bound Ran dissociates the isolated NMD5/TFIIS complex.1 Publication

Protein-protein interaction databases

BioGridi33887. 73 interactions.
DIPiDIP-2326N.
IntActiP46970. 36 interactions.
MINTiMINT-561722.
STRINGi4932.YJR132W.

Structurei

3D structure databases

ProteinModelPortaliP46970.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini24 – 10481Importin N-terminalPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 importin N-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5656.
GeneTreeiENSGT00550000074736.
HOGENOMiHOG000248078.
InParanoidiP46970.
OMAiYKLFKSD.
OrthoDBiEOG7QK0MD.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR001494. Importin-beta_N.
[Graphical view]
PfamiPF03810. IBN_N. 1 hit.
[Graphical view]
SMARTiSM00913. IBN_N. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50166. IMPORTIN_B_NT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P46970 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDITELLQCF ACTLDHNAAV RTNAETHLKN ASKVPGFLGA CLDIIAADEV
60 70 80 90 100
PENIKLSASL YFKNKITYGW SAGARQGSNE LLDSHVDPDE KPVVKDMLIK
110 120 130 140 150
TMVSVSKTSP RCIRVLKSAL TVIISEDYPS KKWGNLLPNS LELLANEDIT
160 170 180 190 200
VTYVGLLCLA EIFRTYRWKN NDERQDLEEL ILNYFPALLN YGANVLFQDG
210 220 230 240 250
KYMNNEQIGE LVKLIIKIYK FVSYHDLPFT LQRSESFTPW ACFFVSIIQQ
260 270 280 290 300
PLPQEVLAIS DIEVRSKNPW VKCKKWALAN LYRLFQRYAS TSLTRKFQYD
310 320 330 340 350
EFKQMYCEEF LTQFLQVVFD QIEKWGTGQL WLSDECLYYI LNFVEQCVVQ
360 370 380 390 400
KTTWKLVGPH YNVILQHVIF PLLKPTAETL EAFDNDPQEY INRNMDFWDV
410 420 430 440 450
GYSPDLAALA LLTTCVTKRG KTTLQPTLEF MVSTLQSAVG DYNNIMLDNA
460 470 480 490 500
LQIESCLRIF SSIIDRLITK DSPFASEMEK FILTYVLPFF KSQYGFLQSR
510 520 530 540 550
VCDICSKLGS MDFKDPVITS TIYEGVMNCL NNSSNSLPVE LTAALALQTF
560 570 580 590 600
ISDDQFNMKL SEHVVPTMQK LLSLSNDFES DVISGVMQDF VEQFAEQLQP
610 620 630 640 650
FGVELMNTLV QQFLKLAIDL HETSNLDPDS FTNVDSIPDE SDKQMAALGI
660 670 680 690 700
LSTTISILLS FENSPEILKN LEQSFYPAAE FILKNDIEDF YRECCEFVEN
710 720 730 740 750
STFLLRDITP ISWKILELIG ECNRKPDSMV SYYLSDFMLA LNNILIYGRN
760 770 780 790 800
ELKKNEFYTK IIFEIYQKAV TAEDNSLDDL RVVFDLSQEL VLALDDSLPQ
810 820 830 840 850
QYRERLLADV VGSILTQKNE LKTNVVFSVT AFNVVISNMI TEPLITLQYL
860 870 880 890 900
KQQGCLEIFF QTWITDYIPN YKRCYDIKLS VLALLKIILK LESNDYSVLN
910 920 930 940 950
LENLVPQLGS IVTQLASRLP TALRQLANQR KEFSSSGFEE DTKWDENFLD
960 970 980 990 1000
VGDDDENDDE GDLTEKYLEL IKNRADSLDF VDGYDAKETF DDLEEDPLTG
1010 1020 1030 1040
SILDTVDVYK VFKESIANLQ HVDSNRYQGI LRHLTPADQE LFMGIMNA
Length:1,048
Mass (Da):119,967
Last modified:September 21, 2011 - v2
Checksum:i90312C1AAE515949
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti258 – 2581A → S in CAA89663. (PubMed:8641269)Curated
Sequence conflicti517 – 5171V → I in AAA74490. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U31375 Genomic DNA. Translation: AAA74490.1.
Z49632 Genomic DNA. Translation: CAA89663.1.
BK006943 Genomic DNA. Translation: DAA08916.2.
PIRiS57155.
RefSeqiNP_012666.2. NM_001181790.2.

Genome annotation databases

EnsemblFungiiYJR132W; YJR132W; YJR132W.
GeneIDi853596.
KEGGisce:YJR132W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U31375 Genomic DNA. Translation: AAA74490.1 .
Z49632 Genomic DNA. Translation: CAA89663.1 .
BK006943 Genomic DNA. Translation: DAA08916.2 .
PIRi S57155.
RefSeqi NP_012666.2. NM_001181790.2.

3D structure databases

ProteinModelPortali P46970.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33887. 73 interactions.
DIPi DIP-2326N.
IntActi P46970. 36 interactions.
MINTi MINT-561722.
STRINGi 4932.YJR132W.

Proteomic databases

MaxQBi P46970.
PaxDbi P46970.
PeptideAtlasi P46970.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YJR132W ; YJR132W ; YJR132W .
GeneIDi 853596.
KEGGi sce:YJR132W.

Organism-specific databases

CYGDi YJR132w.
SGDi S000003893. NMD5.

Phylogenomic databases

eggNOGi COG5656.
GeneTreei ENSGT00550000074736.
HOGENOMi HOG000248078.
InParanoidi P46970.
OMAi YKLFKSD.
OrthoDBi EOG7QK0MD.

Enzyme and pathway databases

BioCyci YEAST:G3O-31750-MONOMER.

Miscellaneous databases

NextBioi 974412.

Gene expression databases

Genevestigatori P46970.

Family and domain databases

Gene3Di 1.25.10.10. 2 hits.
InterProi IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR001494. Importin-beta_N.
[Graphical view ]
Pfami PF03810. IBN_N. 1 hit.
[Graphical view ]
SMARTi SM00913. IBN_N. 1 hit.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 1 hit.
PROSITEi PS50166. IMPORTIN_B_NT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. He F., Jacobson A.
    Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 258.
    Strain: ATCC 204508 / S288c.
  4. "A novel nuclear import pathway for the transcription factor TFIIS."
    Albertini M., Pemberton L.F., Rosenblum J.S., Blobel G.
    J. Cell Biol. 143:1447-1455(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, SUBUNIT.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-977, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-977, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNMD5_YEAST
AccessioniPrimary (citable) accession number: P46970
Secondary accession number(s): D6VWV0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: September 21, 2011
Last modified: October 29, 2014
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 13500 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3